Homology
BLAST of MS009601 vs. NCBI nr
Match:
XP_038897980.1 (subtilisin-like protease SBT1.4 [Benincasa hispida])
HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 682/771 (88.46%), Postives = 728/771 (94.42%), Query Frame = 0
Query: 1 MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
MA H+CS L F F+LCF PVIFSR P++Q+TFIVHVSKSEKP+LFSSHHHWHS+ILESL
Sbjct: 1 MAKSHLCSSLFFLFILCFAPVIFSRSPENQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60
Query: 61 SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
SPS HPTKLLYNYERAVNGFSARLTAAQA +LR VPGV+SVIPDRIRQLHTTRTPHFLGL
Sbjct: 61 SPSPHPTKLLYNYERAVNGFSARLTAAQAAQLRHVPGVISVIPDRIRQLHTTRTPHFLGL 120
Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+SWKG C+TGEGF AS CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASFCN 180
Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
RKIIGARAYFYGYAS+ G+SF SSD +S RDTEGHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAYFYGYASNFGRSFNGSSDFKSARDTEGHGTHTASTAAGSFVKNASFFQYARG 240
Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
EARGMASRARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
GVSLYSGDPLGDSK+PLVYGGDCGSRYCY GSLN SKVAGKIVVCDRGGNARV KGGAVK
Sbjct: 361 GVSLYSGDPLGDSKVPLVYGGDCGSRYCYSGSLNPSKVAGKIVVCDRGGNARVAKGGAVK 420
Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
AGG GM+LANTEENGEELLAD+HLIPGTMVGEIAGNKLRDY+HSDPNPTA +VFRGTVI
Sbjct: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHSDPNPTAIIVFRGTVI 480
Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILA W+G+SSPTGL+IDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILASWSGYSSPTGLNIDPRRVEFNI 540
Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
ISGTSMSCPHVSGVAALLRKAFP WSPAAIKSALITTSYS+D+SGSPIKDL+TSE+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSEESNPF 600
Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
VHGAGHIDPN+ALNPGLIYD++ QDYVSFLCSIGYD +QIAVFVKDSSYSQLC HK +NP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDISPQDYVSFLCSIGYDSKQIAVFVKDSSYSQLCEHKLSNP 660
Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
GNLNYPSFAVVF + E+VVKYTRTVTNVG E+D VYEVKVE+PQGV ISV+PNKL FNA
Sbjct: 661 GNLNYPSFAVVF--NGEDVVKYTRTVTNVGDESDVVYEVKVEAPQGVEISVVPNKLSFNA 720
Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
+KTT SYEITFTKI+GF ++ASFGSI+WSDGSH VRSPIAVSFN+GS+ASM
Sbjct: 721 EKTTDSYEITFTKISGFVEAASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769
BLAST of MS009601 vs. NCBI nr
Match:
XP_022992130.1 (subtilisin-like protease SBT1.4 [Cucurbita maxima])
HSP 1 Score: 1368.2 bits (3540), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 719/771 (93.26%), Query Frame = 0
Query: 1 MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
MANFH CS + FFF LCF+PVIFSR PD Q+TFIVHVSKSEKP+LFSSH+ WHS+IL+SL
Sbjct: 1 MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60
Query: 61 SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
SPS P KLLYNYERAVNGF+AR+TA QA ELRRVPGVV VIPDR+RQLHTTRTPHFLGL
Sbjct: 61 SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120
Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
ADNLGLWPDTNYADDVIIGVLDTGIWPE PSFSDEGLSPVP+SWKG C+ GEGF A+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180
Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
RKIIGARA+F+GY S++G+ SD +S RD++GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG AVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420
Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480
Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540
Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
ISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSGSPIKDL+TS DSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600
Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
VHG GHIDPN+ALNPGLIYDLN DYVSFLCSIGYD RQIAVFVKDSSYS LC HK +NP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660
Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
GNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720
Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
KKTT+SYEITFTKI+GFK SASFGSI+WSDGSH+VRSPIAVSFN+ S+ASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
BLAST of MS009601 vs. NCBI nr
Match:
KAG6575439.1 (Subtilisin-like protease 1.4, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1364.4 bits (3530), Expect = 0.0e+00
Identity = 671/773 (86.80%), Postives = 718/773 (92.88%), Query Frame = 0
Query: 1 MANFHVCS--FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILE 60
MANFH CS F FFF LCF+PVIFSR PD Q+TFIVHVSK EKP+LFSSH+ WHS+IL+
Sbjct: 1 MANFHRCSSVFFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILD 60
Query: 61 SLSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFL 120
SLSPS P KLLYNYERAVNGF+AR+T AQA ELRRVPGVVSVIPDR+RQLHTTRTPHFL
Sbjct: 61 SLSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFL 120
Query: 121 GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASA 180
GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+SWKG C+ GEGF A+
Sbjct: 121 GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATT 180
Query: 181 CNRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYA 240
CNRKIIGARA+F+GY S++G+ SD +S RD++GHGTHTASTAAGSFVKNASF +YA
Sbjct: 181 CNRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYA 240
Query: 241 RGEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD 300
RGEARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD
Sbjct: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD 300
Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
SIAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV
Sbjct: 301 SIAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
Query: 361 FGGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGA 420
F GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG A
Sbjct: 361 FSGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSA 420
Query: 421 VKLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGT 480
VK AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGT
Sbjct: 421 VKSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGT 480
Query: 481 VIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEF 540
VI DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEF
Sbjct: 481 VISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEF 540
Query: 541 NIISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSN 600
NIISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSG PIKDL+TS DSN
Sbjct: 541 NIISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSN 600
Query: 601 PFVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFT 660
PFVHG GHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDSSYS LC HK +
Sbjct: 601 PFVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLS 660
Query: 661 NPGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEF 720
NPGNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF
Sbjct: 661 NPGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEF 720
Query: 721 NAKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
+AKKTTQSYEITFTKI+GFK SASFGSI+WSDGSH VRSPIAVSFN+ S+ASM
Sbjct: 721 SAKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHTVRSPIAVSFNTASIASM 773
BLAST of MS009601 vs. NCBI nr
Match:
XP_022964269.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])
HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 716/771 (92.87%), Query Frame = 0
Query: 1 MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
MAN H+CS FF +LCFVPVIFSR +Q+TFIVHVSKSEKPALFSSHHHWHS+ILESL
Sbjct: 1 MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
Query: 61 SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
SPS HPTKLLY+YE A NGFSAR+TAAQA ELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61 SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVP++WKG CQTG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
RKIIGARAYF GY S + +S + SSD +S RDT+GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
GVSLYSGDPLGDSKLPLVY GDCG RYCY G+L+SSKVAGKIVVCDRGGNARV KG AVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
AGG GMILANTEENGEELLAD+HLIPGTM+GEIAGN L+DY+HSDP+PTAT+VFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G+SSPT LSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
ISGTSMSCPHVSGVAALLRKAFP WSPAAIKSAL+TTSYS+D+SG PI DL+TS+ SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
VHGAGHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDS Y+QLC HK T+P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
GNLNYPSFAVVFDSD +EVVKYTRTVTNVG E DAVYEVKVESPQGV I+VLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
K TQSYEITFTKING K SASFGSI+WSDGSH+VRSP+AVSFNSG +ASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
BLAST of MS009601 vs. NCBI nr
Match:
XP_022953749.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])
HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 669/772 (86.66%), Postives = 718/772 (93.01%), Query Frame = 0
Query: 1 MANFHVC-SFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILES 60
MANFH C S FFF LCF+PVIFSR PD Q+TFIVHVSK EKP+LFSSH+ WHS+IL+S
Sbjct: 1 MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60
Query: 61 LSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLG 120
LSPS P KLLYNYERAVNGF+AR+T AQA ELRRVPGVVSVIPDR+RQLHTTRTPHFLG
Sbjct: 61 LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120
Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASAC 180
LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+SWKG C+ GEGF A+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180
Query: 181 NRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYAR 240
NRKIIGARA+F+GY S++G+ SD +S RD++GHGTHTASTAAGSFVKNASF +YAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240
Query: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
GEARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
Query: 361 GGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 420
GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420
Query: 421 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTV 480
K AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480
Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFN 540
I DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540
Query: 541 IISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNP 600
IISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSG PIKDL+TS DSNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600
Query: 601 FVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTN 660
FVHG GHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDSSYS LC HK +N
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660
Query: 661 PGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFN 720
PGNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF+
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720
Query: 721 AKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
AKKTTQSYEITFTKI+GFK SASFGSI+WSDGSH+VRSPIAVSFN+ S+ASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772
BLAST of MS009601 vs. ExPASy Swiss-Prot
Match:
Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)
HSP 1 Score: 998.8 bits (2581), Expect = 3.4e-290
Identity = 501/769 (65.15%), Postives = 596/769 (77.50%), Query Frame = 0
Query: 9 FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTK 68
F +F LLCF S D +++IVHV +S KP+LFSSH++WH ++L SL S P
Sbjct: 10 FFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPAT 69
Query: 69 LLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWP 128
LLY+Y RAV+GFSARL+ Q LRR P V+SVIPD+ R++HTT TP FLG + N GLW
Sbjct: 70 LLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWS 129
Query: 129 DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARA 188
++NY +DVI+GVLDTGIWPE PSFSD GL P+PS+WKG C+ G FPAS+CNRK+IGARA
Sbjct: 130 NSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARA 189
Query: 189 YFYGYASHL-GKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMAS 248
++ GY + G + + RSPRDTEGHGTHTASTAAGS V NAS +YARG A GMAS
Sbjct: 190 FYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMAS 249
Query: 249 RARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 308
+ARIAAYKICW GCYDSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGAFGA
Sbjct: 250 KARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGA 309
Query: 309 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSG 368
+HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+VF G SLY+G
Sbjct: 310 TRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAG 369
Query: 369 DPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGM 428
+ L DS+L LVY GDCGSR CY G LNSS V GKIV+CDRGGNARVEKG AVKLAGG GM
Sbjct: 370 ESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGM 429
Query: 429 ILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAP 488
ILANT E+GEEL AD+HL+P TMVG AG+++RDY+ + +PTA + F GT+IG SPP+P
Sbjct: 430 ILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSP 489
Query: 489 RVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMS 548
RVAAFSSRGPN+ T ILKPDVIAPGVNILAGWTG PT L IDPRRV+FNIISGTSMS
Sbjct: 490 RVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMS 549
Query: 549 CPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHI 608
CPHVSG+AALLRKA P WSPAAIKSAL+TT+Y V+ SG PI+DLAT + SN F+HGAGH+
Sbjct: 550 CPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHV 609
Query: 609 DPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLC-SHKFTNPGNLNYP 668
DPN+ALNPGL+YD+ ++YV+FLC++GY+ I VF++D + C + K G+LNYP
Sbjct: 610 DPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYP 669
Query: 669 SFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQS 728
SF+VVF S EVVKY R V NVGS DAVYEV V+SP V I V P+KL F+ +K+
Sbjct: 670 SFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLE 729
Query: 729 YEITFTKI-----NGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVAS 771
YE+TF + G FGSIEW+DG HVV+SP+AV + GSV S
Sbjct: 730 YEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776
BLAST of MS009601 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 789.6 bits (2038), Expect = 3.1e-227
Identity = 424/761 (55.72%), Postives = 527/761 (69.25%), Query Frame = 0
Query: 12 FFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLY 71
FF LLC S DQ T+IVH++KS+ P+ F H +W+ + L S+S S +LLY
Sbjct: 11 FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---AELLY 70
Query: 72 NYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLG-LWPDT 131
YE A++GFS RLT +A L PGV+SV+P+ +LHTTRTP FLGL ++ L+P+
Sbjct: 71 TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 130
Query: 132 NYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYF 191
DV++GVLDTG+WPE S+SDEG P+PSSWKG C+ G F AS CNRK+IGAR +
Sbjct: 131 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 190
Query: 192 YGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRAR 251
GY S +G ++S + RSPRD +GHGTHT+STAAGS V+ AS L YA G ARGMA RAR
Sbjct: 191 RGYESTMG-PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRAR 250
Query: 252 IAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQH 311
+A YK+CW GC+ SDILAA+D+AIAD V+++S+S+G G YYRD +AIGAF AM+
Sbjct: 251 VAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAAMER 310
Query: 312 GVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPL 371
G++VSCSAGN+GP + N+APWI TVGA T+DR+F A ILG+G+ F GVSL+ G+ L
Sbjct: 311 GILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL 370
Query: 372 GDSKLPLVYGGDCGS----RYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRG 431
D LP +Y G+ + C G+L KV GKIV+CDRG NARV+KG VK AGG G
Sbjct: 371 PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVG 430
Query: 432 MILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPA 491
MILANT NGEEL+ADAHL+P T VGE AG+ +R YV +DPNPTA++ GTV+G P+
Sbjct: 431 MILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG-VKPS 490
Query: 492 PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSM 551
P VAAFSSRGPN T ILKPD+IAPGVNILA WTG + PTGL+ D RRVEFNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550
Query: 552 SCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGH 611
SCPHVSG+AALL+ P WSPAAI+SAL+TT+Y G P+ D+AT + S PF HGAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610
Query: 612 IDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCS-HKFTNPGNLNY 671
+ P A NPGLIYDL ++DY+ FLC++ Y QI S + C K + +LNY
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVADLNY 670
Query: 672 PSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVES-PQGVLISVLPNKLEFNAKKTT 731
PSFAV + D KYTRTVT+VG Y VKV S GV ISV P L F
Sbjct: 671 PSFAV--NVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKEANEK 730
Query: 732 QSYEITFTKINGFKKSA--SFGSIEWSDGSHVVRSPIAVSF 764
+SY +TFT ++ K S SFGSIEWSDG HVV SP+A+S+
Sbjct: 731 KSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756
BLAST of MS009601 vs. ExPASy Swiss-Prot
Match:
Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)
HSP 1 Score: 760.0 bits (1961), Expect = 2.6e-218
Identity = 398/740 (53.78%), Postives = 512/740 (69.19%), Query Frame = 0
Query: 30 QQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLYNYERAVNGFSARLTAAQA 89
++T+I+ V+ S+KP F +HH W+++ L S S LLY Y + +GFSA L + +A
Sbjct: 27 KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86
Query: 90 GE-LRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWPDTNYADDVIIGVLDTGIWPE 149
L ++ + D + LHTTRTP FLGL G+ + ++ VIIGVLDTG+WPE
Sbjct: 87 DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146
Query: 150 RPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYFYGYASHLGKSFEDSSDLR 209
SF D + +PS WKG C++G F + CN+K+IGAR++ G+ G F +
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206
Query: 210 SPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRARIAAYKICWELGCYDSDIL 269
SPRD +GHGTHT++TAAGS V+NASFL YA G ARGMA+RAR+A YK+CW GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266
Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
AAMD+AI DGV ++SLS+G G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP +
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326
Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPLGDSKLPLVY--GGDCGSR 389
N+APW++TVGA T+DR+F A LG+G+ GVSLYSG +G L LVY G S
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386
Query: 390 YCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGMILANTEENGEELLADAHLI 449
C GSL+SS V GKIVVCDRG NARVEKG V+ AGG GMI+ANT +GEEL+AD+HL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446
Query: 450 PGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILK 509
P VG+ G+ LR+YV SD PTA LVF+GTV+ D P+P VAAFSSRGPN T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506
Query: 510 PDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPAWS 569
PDVI PGVNILAGW+ PTGL D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566
Query: 570 PAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDY 629
P+AIKSAL+TT+Y +D + +P+ D A + SNP+ HG+GH+DP +AL+PGL+YD+++++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626
Query: 630 VSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNPGNLNYPSFAVVFDSDAEEVVKYTRTV 689
+ FLCS+ Y I VK S + CS KF++PG LNYPSF+V+F + VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686
Query: 690 TNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQSYEITFTKING--FKKSASFG 749
TNVG+ A +VY+V V V ISV P+KL F + + Y +TF G A FG
Sbjct: 687 TNVGA-ASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746
Query: 750 SIEWSDGSHVVRSPIAVSFN 765
SI WS+ H VRSP+A S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752
BLAST of MS009601 vs. ExPASy Swiss-Prot
Match:
Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)
HSP 1 Score: 728.4 bits (1879), Expect = 8.4e-209
Identity = 389/778 (50.00%), Postives = 515/778 (66.20%), Query Frame = 0
Query: 9 FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTK 68
F FFFLL S + T+IVHV KP++F +H HW+++ L SL+ S P
Sbjct: 4 FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPS 63
Query: 69 LLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL--ADNLGL 128
+++ Y+ +GFSARLT+ A +L P V+SVIP+++R LHTTR+P FLGL D GL
Sbjct: 64 IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGL 123
Query: 129 WPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGA 188
++++ D++IGV+DTG+WPERPSF D GL PVP WKG C + FP SACNRK++GA
Sbjct: 124 LEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGA 183
Query: 189 RAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMA 248
R + GY + GK ++++ RSPRD++GHGTHTAS +AG +V AS L YA G A GMA
Sbjct: 184 RFFCGGYEATNGK-MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 243
Query: 249 SRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFG 308
+AR+AAYK+CW GCYDSDILAA D A+ADGV +ISLSVG G YY D+IAIGAFG
Sbjct: 244 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFG 303
Query: 309 AMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYS 368
A+ G+ VS SAGN GPG T N+APW+ TVGA TIDR+F A+V LG+G++ GVS+Y
Sbjct: 304 AIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYG 363
Query: 369 GDPLGDSKL-PLVYGGDC------GSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 428
G L ++ PLVYGG S C GSL+ + V GKIV+CDRG N+R KG V
Sbjct: 364 GPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIV 423
Query: 429 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYV------HSDPNPTATL 488
+ GG GMI+AN +GE L+AD H++P T VG G+++R Y+ S +PTAT+
Sbjct: 424 RKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATI 483
Query: 489 VFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDP 548
VF+GT +G PAP VA+FS+RGPN T EILKPDVIAPG+NILA W P+G++ D
Sbjct: 484 VFKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 543
Query: 549 RRVEFNIISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLAT 608
RR EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y+VD SG P+ D +T
Sbjct: 544 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDEST 603
Query: 609 SEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVK---DSSYS 668
S+ +G+GH+ P +A++PGL+YD+ S DY++FLC+ Y I + D +
Sbjct: 604 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 663
Query: 669 QLCSHKFTNPGNLNYPSFAVVFDSDAEEVVK--YTRTVTNVGSEADAVYEVKVESPQGVL 728
+ H GNLNYPSF+VVF E + + RTVTNVG ++D+VYE+K+ P+G
Sbjct: 664 RRAGH----VGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVG-DSDSVYEIKIRPPRGTT 723
Query: 729 ISVLPNKLEFNAKKTTQSYEI----TFTKINGFKKSASFGSIEWSDGSHVVRSPIAVS 763
++V P KL F S+ + T K++ + G I WSDG V SP+ V+
Sbjct: 724 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770
BLAST of MS009601 vs. ExPASy Swiss-Prot
Match:
O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)
HSP 1 Score: 707.2 bits (1824), Expect = 2.0e-202
Identity = 377/769 (49.02%), Postives = 496/769 (64.50%), Query Frame = 0
Query: 6 VCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLH 65
+ S I L P I +TFI + P++F +H+HW+ST S
Sbjct: 1 MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES---- 60
Query: 66 PTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLG 125
++++ Y +GFSA +T +A LR P V++V DR R+LHTTR+P FLGL + G
Sbjct: 61 --RIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG 120
Query: 126 LWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIG 185
LW +++Y DVIIGV DTGIWPER SFSD L P+P W+G+C++G F CNRKIIG
Sbjct: 121 LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIG 180
Query: 186 ARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGM 245
AR + G + + + + SPRD +GHGTHT+STAAG AS YA G A+G+
Sbjct: 181 ARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGV 240
Query: 246 ASRARIAAYKICW-ELGCYDSDILAAMDQAIADGVHIISLSV-GSSGRAPAYYRDSIAIG 305
A +ARIAAYK+CW + GC DSDILAA D A+ DGV +IS+S+ G G YY D IAIG
Sbjct: 241 APKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIG 300
Query: 306 AFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVS 365
++GA G+ VS SAGN GP + N+APW+ TVGASTIDR F AD ILGDG GVS
Sbjct: 301 SYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVS 360
Query: 366 LYSGDPLGDSKLPLVYGGDCG---SRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 425
LY+G PL P+VY G G + C +L+ +V GKIV+CDRG + RV KG VK
Sbjct: 361 LYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 420
Query: 426 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 485
AGG GMILAN NGE L+ DAHLIP VG G++++ Y S PNP A++ FRGT++
Sbjct: 421 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 480
Query: 486 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 545
G PAP +A+FS RGPN + EILKPD+IAPGVNILA WT PTGL DPR+ EFNI
Sbjct: 481 G-IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNI 540
Query: 546 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 605
+SGTSM+CPHVSG AALL+ A P WSPA I+SA++TT+ VD S + D +T + + P+
Sbjct: 541 LSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPY 600
Query: 606 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 665
+G+GH++ RA+NPGL+YD+ + DY++FLCSIGY P+ I V + + + + + +P
Sbjct: 601 DYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPSP 660
Query: 666 GNLNYPSFAVVFDSDAEEVVKYT--RTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEF 725
GNLNYPS VF ++ +V T RT TNVG +A+AVY ++ESP+GV ++V P +L F
Sbjct: 661 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLVF 720
Query: 726 NAKKTTQSYEITFT----KINGFKKSASFGSIEWSD-GSHVVRSPIAVS 763
+ +SY +T T + + A FGS+ W D G HVVRSPI V+
Sbjct: 721 TSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759
BLAST of MS009601 vs. ExPASy TrEMBL
Match:
A0A6J1JUV1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3 SV=1)
HSP 1 Score: 1368.2 bits (3540), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 719/771 (93.26%), Query Frame = 0
Query: 1 MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
MANFH CS + FFF LCF+PVIFSR PD Q+TFIVHVSKSEKP+LFSSH+ WHS+IL+SL
Sbjct: 1 MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60
Query: 61 SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
SPS P KLLYNYERAVNGF+AR+TA QA ELRRVPGVV VIPDR+RQLHTTRTPHFLGL
Sbjct: 61 SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120
Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
ADNLGLWPDTNYADDVIIGVLDTGIWPE PSFSDEGLSPVP+SWKG C+ GEGF A+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180
Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
RKIIGARA+F+GY S++G+ SD +S RD++GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240
Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360
Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG AVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420
Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480
Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540
Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
ISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSGSPIKDL+TS DSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600
Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
VHG GHIDPN+ALNPGLIYDLN DYVSFLCSIGYD RQIAVFVKDSSYS LC HK +NP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660
Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
GNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720
Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
KKTT+SYEITFTKI+GFK SASFGSI+WSDGSH+VRSPIAVSFN+ S+ASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771
BLAST of MS009601 vs. ExPASy TrEMBL
Match:
A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)
HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 716/771 (92.87%), Query Frame = 0
Query: 1 MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
MAN H+CS FF +LCFVPVIFSR +Q+TFIVHVSKSEKPALFSSHHHWHS+ILESL
Sbjct: 1 MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
Query: 61 SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
SPS HPTKLLY+YE A NGFSAR+TAAQA ELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61 SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVP++WKG CQTG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
RKIIGARAYF GY S + +S + SSD +S RDT+GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
GVSLYSGDPLGDSKLPLVY GDCG RYCY G+L+SSKVAGKIVVCDRGGNARV KG AVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
AGG GMILANTEENGEELLAD+HLIPGTM+GEIAGN L+DY+HSDP+PTAT+VFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G+SSPT LSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
ISGTSMSCPHVSGVAALLRKAFP WSPAAIKSAL+TTSYS+D+SG PI DL+TS+ SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
VHGAGHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDS Y+QLC HK T+P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
GNLNYPSFAVVFDSD +EVVKYTRTVTNVG E DAVYEVKVESPQGV I+VLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
K TQSYEITFTKING K SASFGSI+WSDGSH+VRSP+AVSFNSG +ASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
BLAST of MS009601 vs. ExPASy TrEMBL
Match:
A0A6J1GQK5 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE=3 SV=1)
HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 669/772 (86.66%), Postives = 718/772 (93.01%), Query Frame = 0
Query: 1 MANFHVC-SFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILES 60
MANFH C S FFF LCF+PVIFSR PD Q+TFIVHVSK EKP+LFSSH+ WHS+IL+S
Sbjct: 1 MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60
Query: 61 LSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLG 120
LSPS P KLLYNYERAVNGF+AR+T AQA ELRRVPGVVSVIPDR+RQLHTTRTPHFLG
Sbjct: 61 LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120
Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASAC 180
LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+SWKG C+ GEGF A+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180
Query: 181 NRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYAR 240
NRKIIGARA+F+GY S++G+ SD +S RD++GHGTHTASTAAGSFVKNASF +YAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240
Query: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
GEARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
Query: 361 GGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 420
GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420
Query: 421 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTV 480
K AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480
Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFN 540
I DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540
Query: 541 IISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNP 600
IISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSG PIKDL+TS DSNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600
Query: 601 FVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTN 660
FVHG GHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDSSYS LC HK +N
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660
Query: 661 PGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFN 720
PGNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF+
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720
Query: 721 AKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
AKKTTQSYEITFTKI+GFK SASFGSI+WSDGSH+VRSPIAVSFN+ S+ASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772
BLAST of MS009601 vs. ExPASy TrEMBL
Match:
A0A6J1KIR1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111494328 PE=3 SV=1)
HSP 1 Score: 1360.9 bits (3521), Expect = 0.0e+00
Identity = 665/771 (86.25%), Postives = 717/771 (93.00%), Query Frame = 0
Query: 1 MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
MAN H+CS + FF +LCFVPVIFSR +Q+TFIVHVSKSEKPALFSSHHHWHS+IL+SL
Sbjct: 1 MANLHLCSSVFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDSL 60
Query: 61 SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
SPS HPTKLLYNYE A NGFSAR+TAAQA ELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61 SPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVP++WKG CQTG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
RKIIGARAYF GY S + +S + SSD +S RDT+GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
EARGMA+RARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
GVSLYSGDPLGDSKLPLVY GDCG+RYCY G+L+SSKVAGKIVVCDRGGNARV KG VK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTVK 420
Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
AGG GMILANTEENGEELLAD+HLIPGTM+GEIAGN L+DY+HSDP+PTAT+VFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
GDSPPAPRVAAFSSRGPN+RTAEILKPDVIAPGVNILAGW+G+SSPT LSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
ISGTSMSCPHVSGVAALLRKAFP WSPAAIKSAL+TTSYS+D+SG PI DL+TS+ SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
VHGAGHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDS Y++LC HK T+P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTSP 660
Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
GNLNYPSFAVVFDSD +EVVKYTRTVTNVG E DAVYEVKVESP+GV I+VLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFSA 720
Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
K TQSYEITFTKING K SASFGSI+WSDGSH+VRSP+AVSFNSG +ASM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
BLAST of MS009601 vs. ExPASy TrEMBL
Match:
A0A0A0K6Z1 (Subtilase family protein OS=Cucumis sativus OX=3659 GN=Csa_7G448910 PE=3 SV=1)
HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 667/773 (86.29%), Postives = 712/773 (92.11%), Query Frame = 0
Query: 1 MANFHV-CSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILES 60
MANFH+ S FFFL CF P+IFSR ++Q+T+IVHVSKSEKP+LFSSHHHWHS+ILES
Sbjct: 1 MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60
Query: 61 LSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLG 120
LS S HPTKLLYNYERA NGFSAR+T QA ELRRVPG++SVIPD+IRQLHTTRTPHFLG
Sbjct: 61 LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120
Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASAC 180
LADNLGLW DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+ WKG C TGEG A AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180
Query: 181 NRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYAR 240
NRKIIGARAYFYGY S+L S + SSD +S RDTEGHGTHTASTAAGSFV NASF +YAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240
Query: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
GEARGMASRARIAAYKICWE GCYDSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300
Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
Query: 361 GGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 420
GVSLYSGDPLGDSKL LVYGGDCGSRYCY GSL+SSKVAGKIVVCDRGGNARV KGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420
Query: 421 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTV 480
K AGG GM+LANTEENGEELLAD+HLIPGTMVG IAGNKLRDY+H+DPNPTAT+VFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480
Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFN 540
IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G+SSPTGL+IDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540
Query: 541 IISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNP 600
IISGTSMSCPHVSGVAALLRKAFP WSPAAIKSALITTSYS+D+SG PIKDL+TSE+SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600
Query: 601 FVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTN 660
FVHGAGHI+PN+ALNPGLIYDL QDYVSFLCSIGYD +QIAVFVK SSY QLC HK TN
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660
Query: 661 PGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFN 720
PGNLNYPSF+VVFD EEVVKYTRTVTNVG E + VYEVKVE+PQGV+ISV+PNKLEFN
Sbjct: 661 PGNLNYPSFSVVFDE--EEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFN 720
Query: 721 AKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNS-GSVASM 772
+KTTQSYEITFTKINGFK+SASFGSI+W DG H VRSPIAVSF + GS+ASM
Sbjct: 721 KEKTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771
BLAST of MS009601 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 998.8 bits (2581), Expect = 2.4e-291
Identity = 501/769 (65.15%), Postives = 596/769 (77.50%), Query Frame = 0
Query: 9 FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTK 68
F +F LLCF S D +++IVHV +S KP+LFSSH++WH ++L SL S P
Sbjct: 10 FFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPAT 69
Query: 69 LLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWP 128
LLY+Y RAV+GFSARL+ Q LRR P V+SVIPD+ R++HTT TP FLG + N GLW
Sbjct: 70 LLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWS 129
Query: 129 DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARA 188
++NY +DVI+GVLDTGIWPE PSFSD GL P+PS+WKG C+ G FPAS+CNRK+IGARA
Sbjct: 130 NSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARA 189
Query: 189 YFYGYASHL-GKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMAS 248
++ GY + G + + RSPRDTEGHGTHTASTAAGS V NAS +YARG A GMAS
Sbjct: 190 FYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMAS 249
Query: 249 RARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 308
+ARIAAYKICW GCYDSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGAFGA
Sbjct: 250 KARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGA 309
Query: 309 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSG 368
+HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+VF G SLY+G
Sbjct: 310 TRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAG 369
Query: 369 DPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGM 428
+ L DS+L LVY GDCGSR CY G LNSS V GKIV+CDRGGNARVEKG AVKLAGG GM
Sbjct: 370 ESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGM 429
Query: 429 ILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAP 488
ILANT E+GEEL AD+HL+P TMVG AG+++RDY+ + +PTA + F GT+IG SPP+P
Sbjct: 430 ILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSP 489
Query: 489 RVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMS 548
RVAAFSSRGPN+ T ILKPDVIAPGVNILAGWTG PT L IDPRRV+FNIISGTSMS
Sbjct: 490 RVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMS 549
Query: 549 CPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHI 608
CPHVSG+AALLRKA P WSPAAIKSAL+TT+Y V+ SG PI+DLAT + SN F+HGAGH+
Sbjct: 550 CPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHV 609
Query: 609 DPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLC-SHKFTNPGNLNYP 668
DPN+ALNPGL+YD+ ++YV+FLC++GY+ I VF++D + C + K G+LNYP
Sbjct: 610 DPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYP 669
Query: 669 SFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQS 728
SF+VVF S EVVKY R V NVGS DAVYEV V+SP V I V P+KL F+ +K+
Sbjct: 670 SFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLE 729
Query: 729 YEITFTKI-----NGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVAS 771
YE+TF + G FGSIEW+DG HVV+SP+AV + GSV S
Sbjct: 730 YEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776
BLAST of MS009601 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 789.6 bits (2038), Expect = 2.2e-228
Identity = 424/761 (55.72%), Postives = 527/761 (69.25%), Query Frame = 0
Query: 12 FFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLY 71
FF LLC S DQ T+IVH++KS+ P+ F H +W+ + L S+S S +LLY
Sbjct: 11 FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---AELLY 70
Query: 72 NYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLG-LWPDT 131
YE A++GFS RLT +A L PGV+SV+P+ +LHTTRTP FLGL ++ L+P+
Sbjct: 71 TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 130
Query: 132 NYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYF 191
DV++GVLDTG+WPE S+SDEG P+PSSWKG C+ G F AS CNRK+IGAR +
Sbjct: 131 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 190
Query: 192 YGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRAR 251
GY S +G ++S + RSPRD +GHGTHT+STAAGS V+ AS L YA G ARGMA RAR
Sbjct: 191 RGYESTMG-PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRAR 250
Query: 252 IAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQH 311
+A YK+CW GC+ SDILAA+D+AIAD V+++S+S+G G YYRD +AIGAF AM+
Sbjct: 251 VAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAAMER 310
Query: 312 GVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPL 371
G++VSCSAGN+GP + N+APWI TVGA T+DR+F A ILG+G+ F GVSL+ G+ L
Sbjct: 311 GILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL 370
Query: 372 GDSKLPLVYGGDCGS----RYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRG 431
D LP +Y G+ + C G+L KV GKIV+CDRG NARV+KG VK AGG G
Sbjct: 371 PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVG 430
Query: 432 MILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPA 491
MILANT NGEEL+ADAHL+P T VGE AG+ +R YV +DPNPTA++ GTV+G P+
Sbjct: 431 MILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG-VKPS 490
Query: 492 PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSM 551
P VAAFSSRGPN T ILKPD+IAPGVNILA WTG + PTGL+ D RRVEFNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550
Query: 552 SCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGH 611
SCPHVSG+AALL+ P WSPAAI+SAL+TT+Y G P+ D+AT + S PF HGAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610
Query: 612 IDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCS-HKFTNPGNLNY 671
+ P A NPGLIYDL ++DY+ FLC++ Y QI S + C K + +LNY
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVADLNY 670
Query: 672 PSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVES-PQGVLISVLPNKLEFNAKKTT 731
PSFAV + D KYTRTVT+VG Y VKV S GV ISV P L F
Sbjct: 671 PSFAV--NVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKEANEK 730
Query: 732 QSYEITFTKINGFKKSA--SFGSIEWSDGSHVVRSPIAVSF 764
+SY +TFT ++ K S SFGSIEWSDG HVV SP+A+S+
Sbjct: 731 KSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756
BLAST of MS009601 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 760.0 bits (1961), Expect = 1.9e-219
Identity = 398/740 (53.78%), Postives = 512/740 (69.19%), Query Frame = 0
Query: 30 QQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLYNYERAVNGFSARLTAAQA 89
++T+I+ V+ S+KP F +HH W+++ L S S LLY Y + +GFSA L + +A
Sbjct: 27 KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86
Query: 90 GE-LRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWPDTNYADDVIIGVLDTGIWPE 149
L ++ + D + LHTTRTP FLGL G+ + ++ VIIGVLDTG+WPE
Sbjct: 87 DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146
Query: 150 RPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYFYGYASHLGKSFEDSSDLR 209
SF D + +PS WKG C++G F + CN+K+IGAR++ G+ G F +
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206
Query: 210 SPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRARIAAYKICWELGCYDSDIL 269
SPRD +GHGTHT++TAAGS V+NASFL YA G ARGMA+RAR+A YK+CW GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266
Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
AAMD+AI DGV ++SLS+G G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP +
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326
Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPLGDSKLPLVY--GGDCGSR 389
N+APW++TVGA T+DR+F A LG+G+ GVSLYSG +G L LVY G S
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386
Query: 390 YCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGMILANTEENGEELLADAHLI 449
C GSL+SS V GKIVVCDRG NARVEKG V+ AGG GMI+ANT +GEEL+AD+HL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446
Query: 450 PGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILK 509
P VG+ G+ LR+YV SD PTA LVF+GTV+ D P+P VAAFSSRGPN T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506
Query: 510 PDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPAWS 569
PDVI PGVNILAGW+ PTGL D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566
Query: 570 PAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDY 629
P+AIKSAL+TT+Y +D + +P+ D A + SNP+ HG+GH+DP +AL+PGL+YD+++++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626
Query: 630 VSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNPGNLNYPSFAVVFDSDAEEVVKYTRTV 689
+ FLCS+ Y I VK S + CS KF++PG LNYPSF+V+F + VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686
Query: 690 TNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQSYEITFTKING--FKKSASFG 749
TNVG+ A +VY+V V V ISV P+KL F + + Y +TF G A FG
Sbjct: 687 TNVGA-ASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746
Query: 750 SIEWSDGSHVVRSPIAVSFN 765
SI WS+ H VRSP+A S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752
BLAST of MS009601 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 728.4 bits (1879), Expect = 6.0e-210
Identity = 389/778 (50.00%), Postives = 515/778 (66.20%), Query Frame = 0
Query: 9 FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTK 68
F FFFLL S + T+IVHV KP++F +H HW+++ L SL+ S P
Sbjct: 4 FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPS 63
Query: 69 LLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL--ADNLGL 128
+++ Y+ +GFSARLT+ A +L P V+SVIP+++R LHTTR+P FLGL D GL
Sbjct: 64 IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGL 123
Query: 129 WPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGA 188
++++ D++IGV+DTG+WPERPSF D GL PVP WKG C + FP SACNRK++GA
Sbjct: 124 LEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGA 183
Query: 189 RAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMA 248
R + GY + GK ++++ RSPRD++GHGTHTAS +AG +V AS L YA G A GMA
Sbjct: 184 RFFCGGYEATNGK-MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 243
Query: 249 SRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFG 308
+AR+AAYK+CW GCYDSDILAA D A+ADGV +ISLSVG G YY D+IAIGAFG
Sbjct: 244 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFG 303
Query: 309 AMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYS 368
A+ G+ VS SAGN GPG T N+APW+ TVGA TIDR+F A+V LG+G++ GVS+Y
Sbjct: 304 AIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYG 363
Query: 369 GDPLGDSKL-PLVYGGDC------GSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 428
G L ++ PLVYGG S C GSL+ + V GKIV+CDRG N+R KG V
Sbjct: 364 GPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIV 423
Query: 429 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYV------HSDPNPTATL 488
+ GG GMI+AN +GE L+AD H++P T VG G+++R Y+ S +PTAT+
Sbjct: 424 RKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATI 483
Query: 489 VFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDP 548
VF+GT +G PAP VA+FS+RGPN T EILKPDVIAPG+NILA W P+G++ D
Sbjct: 484 VFKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 543
Query: 549 RRVEFNIISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLAT 608
RR EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y+VD SG P+ D +T
Sbjct: 544 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDEST 603
Query: 609 SEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVK---DSSYS 668
S+ +G+GH+ P +A++PGL+YD+ S DY++FLC+ Y I + D +
Sbjct: 604 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 663
Query: 669 QLCSHKFTNPGNLNYPSFAVVFDSDAEEVVK--YTRTVTNVGSEADAVYEVKVESPQGVL 728
+ H GNLNYPSF+VVF E + + RTVTNVG ++D+VYE+K+ P+G
Sbjct: 664 RRAGH----VGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVG-DSDSVYEIKIRPPRGTT 723
Query: 729 ISVLPNKLEFNAKKTTQSYEI----TFTKINGFKKSASFGSIEWSDGSHVVRSPIAVS 763
++V P KL F S+ + T K++ + G I WSDG V SP+ V+
Sbjct: 724 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770
BLAST of MS009601 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 707.2 bits (1824), Expect = 1.4e-203
Identity = 377/769 (49.02%), Postives = 496/769 (64.50%), Query Frame = 0
Query: 6 VCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLH 65
+ S I L P I +TFI + P++F +H+HW+ST S
Sbjct: 1 MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES---- 60
Query: 66 PTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLG 125
++++ Y +GFSA +T +A LR P V++V DR R+LHTTR+P FLGL + G
Sbjct: 61 --RIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG 120
Query: 126 LWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIG 185
LW +++Y DVIIGV DTGIWPER SFSD L P+P W+G+C++G F CNRKIIG
Sbjct: 121 LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIG 180
Query: 186 ARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGM 245
AR + G + + + + SPRD +GHGTHT+STAAG AS YA G A+G+
Sbjct: 181 ARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGV 240
Query: 246 ASRARIAAYKICW-ELGCYDSDILAAMDQAIADGVHIISLSV-GSSGRAPAYYRDSIAIG 305
A +ARIAAYK+CW + GC DSDILAA D A+ DGV +IS+S+ G G YY D IAIG
Sbjct: 241 APKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIG 300
Query: 306 AFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVS 365
++GA G+ VS SAGN GP + N+APW+ TVGASTIDR F AD ILGDG GVS
Sbjct: 301 SYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVS 360
Query: 366 LYSGDPLGDSKLPLVYGGDCG---SRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 425
LY+G PL P+VY G G + C +L+ +V GKIV+CDRG + RV KG VK
Sbjct: 361 LYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 420
Query: 426 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 485
AGG GMILAN NGE L+ DAHLIP VG G++++ Y S PNP A++ FRGT++
Sbjct: 421 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 480
Query: 486 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 545
G PAP +A+FS RGPN + EILKPD+IAPGVNILA WT PTGL DPR+ EFNI
Sbjct: 481 G-IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNI 540
Query: 546 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 605
+SGTSM+CPHVSG AALL+ A P WSPA I+SA++TT+ VD S + D +T + + P+
Sbjct: 541 LSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPY 600
Query: 606 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 665
+G+GH++ RA+NPGL+YD+ + DY++FLCSIGY P+ I V + + + + + +P
Sbjct: 601 DYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPSP 660
Query: 666 GNLNYPSFAVVFDSDAEEVVKYT--RTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEF 725
GNLNYPS VF ++ +V T RT TNVG +A+AVY ++ESP+GV ++V P +L F
Sbjct: 661 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLVF 720
Query: 726 NAKKTTQSYEITFT----KINGFKKSASFGSIEWSD-GSHVVRSPIAVS 763
+ +SY +T T + + A FGS+ W D G HVVRSPI V+
Sbjct: 721 TSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9LVJ1 | 3.4e-290 | 65.15 | Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... | [more] |
O65351 | 3.1e-227 | 55.72 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
Q9ZUF6 | 2.6e-218 | 53.78 | Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... | [more] |
Q9LUM3 | 8.4e-209 | 50.00 | Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... | [more] |
O49607 | 2.0e-202 | 49.02 | Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1JUV1 | 0.0e+00 | 86.90 | subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3... | [more] |
A0A6J1HMN7 | 0.0e+00 | 86.90 | subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE... | [more] |
A0A6J1GQK5 | 0.0e+00 | 86.66 | subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE... | [more] |
A0A6J1KIR1 | 0.0e+00 | 86.25 | subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111494328 PE=3... | [more] |
A0A0A0K6Z1 | 0.0e+00 | 86.29 | Subtilase family protein OS=Cucumis sativus OX=3659 GN=Csa_7G448910 PE=3 SV=1 | [more] |