MS009601 (gene) Bitter gourd (TR) v1

Overview
NameMS009601
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionsubtilisin-like protease SBT1.4
Locationscaffold813: 2610459 .. 2612771 (-)
RNA-Seq ExpressionMS009601
SyntenyMS009601
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAACTTCCATGTCTGTTCTTTCCTTATCTTCTTCTTCCTCCTCTGTTTCGTTCCGGTGATTTTCTCCCGTTTGCCGGACGATCAGCAGACCTTCATCGTTCACGTTTCTAAATCGGAGAAGCCTGCTCTCTTCTCCTCTCATCATCATTGGCACTCCACCATTCTCGAATCGCTCTCTCCTTCCCTTCATCCAACGAAGCTTCTTTACAACTACGAACGCGCCGTCAACGGCTTCTCCGCCCGCCTCACGGCTGCTCAGGCGGGGGAGCTGCGTCGCGTTCCTGGTGTTGTTTCGGTGATTCCTGATCGGATCCGTCAGCTTCATACGACTCGAACGCCTCATTTCTTAGGTCTCGCTGATAATCTAGGGCTCTGGCCCGACACGAACTATGCGGATGATGTGATTATTGGCGTTCTTGACACCGGAATTTGGCCCGAACGGCCCAGTTTCTCGGACGAGGGCCTTTCTCCGGTTCCGTCAAGCTGGAAAGGCATCTGCCAGACCGGGGAGGGGTTTCCGGCTTCTGCCTGTAATCGGAAGATTATCGGCGCGAGGGCGTACTTCTATGGATACGCGTCTCATTTAGGAAAATCGTTTGAAGATTCTTCGGATTTGAGATCTCCTAGGGATACGGAGGGCCATGGAACTCACACAGCTTCTACGGCCGCAGGGTCGTTTGTTAAGAACGCGAGTTTTCTTGAATACGCTCGTGGAGAAGCCAGAGGCATGGCGAGCAGAGCTCGAATCGCCGCCTACAAGATCTGCTGGGAACTTGGTTGTTACGACTCCGACATACTCGCCGCTATGGATCAGGCGATTGCCGACGGGGTCCACATCATCTCTCTCTCCGTCGGATCCTCCGGCCGTGCCCCTGCGTACTATCGAGATTCCATCGCAATCGGAGCGTTCGGCGCAATGCAGCACGGCGTTGTCGTCTCTTGCTCAGCCGGAAACTCCGGTCCCGGTCCCTACACGGCGGTGAACATCGCTCCATGGATCCTCACCGTCGGCGCCTCCACAATCGACAGAGAGTTTCTCGCAGATGTGATTCTTGGAGACGGTAGGGTTTTCGGCGGCGTATCCCTCTATTCCGGCGATCCTCTCGGCGATTCCAAGCTTCCGTTGGTCTACGGTGGAGATTGCGGAAGCAGGTATTGTTATTACGGAAGCCTCAATTCATCGAAAGTCGCCGGAAAGATCGTAGTATGCGATCGAGGAGGGAACGCCAGAGTCGAAAAAGGAGGCGCCGTGAAATTAGCCGGCGGTCGTGGTATGATTCTCGCCAACACGGAAGAAAACGGCGAAGAACTCTTGGCGGATGCTCATCTTATACCGGGAACAATGGTGGGCGAAATCGCCGGTAACAAACTCAGAGACTACGTCCATTCAGATCCGAATCCAACTGCAACCCTCGTTTTCCGCGGAACCGTGATCGGCGATTCACCACCGGCGCCCAGAGTCGCCGCCTTCTCCAGCCGCGGTCCGAACTATCGAACGGCAGAGATATTGAAGCCGGACGTGATAGCGCCGGGGGTTAACATTCTCGCCGGCTGGACCGGGTTCAGCAGCCCGACCGGTCTGAGCATCGATCCTCGAAGAGTGGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCACGTCAGCGGCGTCGCCGCCTTGCTCCGAAAGGCTTTTCCGGCATGGTCGCCGGCGGCGATCAAATCGGCTCTGATCACCACTTCTTACTCTGTGGACACTTCCGGCAGCCCAATCAAAGATCTCGCCACGAGTGAAGATTCAAATCCGTTCGTCCACGGTGCCGGCCATATCGATCCCAACAGAGCTCTGAATCCCGGTTTGATTTACGACCTCAATTCTCAGGATTACGTATCATTTCTCTGCTCCATTGGCTACGATCCTCGACAAATAGCTGTTTTCGTGAAGGATTCTTCATACTCGCAACTTTGTTCGCACAAATTCACCAATCCCGGAAATCTGAACTACCCATCATTCGCCGTCGTGTTCGACTCCGACGCCGAGGAGGTGGTGAAATACACAAGAACTGTCACGAACGTCGGAAGCGAAGCCGATGCAGTTTATGAAGTGAAAGTAGAATCCCCACAAGGGGTACTAATCAGCGTTCTTCCAAACAAACTGGAGTTCAACGCGAAGAAGACGACACAGTCTTACGAGATTACATTCACCAAAATCAATGGATTCAAGAAATCGGCGAGCTTCGGATCAATTGAATGGAGCGATGGAAGTCACGTGGTGAGGAGTCCGATTGCTGTGTCGTTCAACAGCGGATCTGTAGCTTCGATG

mRNA sequence

ATGGCGAACTTCCATGTCTGTTCTTTCCTTATCTTCTTCTTCCTCCTCTGTTTCGTTCCGGTGATTTTCTCCCGTTTGCCGGACGATCAGCAGACCTTCATCGTTCACGTTTCTAAATCGGAGAAGCCTGCTCTCTTCTCCTCTCATCATCATTGGCACTCCACCATTCTCGAATCGCTCTCTCCTTCCCTTCATCCAACGAAGCTTCTTTACAACTACGAACGCGCCGTCAACGGCTTCTCCGCCCGCCTCACGGCTGCTCAGGCGGGGGAGCTGCGTCGCGTTCCTGGTGTTGTTTCGGTGATTCCTGATCGGATCCGTCAGCTTCATACGACTCGAACGCCTCATTTCTTAGGTCTCGCTGATAATCTAGGGCTCTGGCCCGACACGAACTATGCGGATGATGTGATTATTGGCGTTCTTGACACCGGAATTTGGCCCGAACGGCCCAGTTTCTCGGACGAGGGCCTTTCTCCGGTTCCGTCAAGCTGGAAAGGCATCTGCCAGACCGGGGAGGGGTTTCCGGCTTCTGCCTGTAATCGGAAGATTATCGGCGCGAGGGCGTACTTCTATGGATACGCGTCTCATTTAGGAAAATCGTTTGAAGATTCTTCGGATTTGAGATCTCCTAGGGATACGGAGGGCCATGGAACTCACACAGCTTCTACGGCCGCAGGGTCGTTTGTTAAGAACGCGAGTTTTCTTGAATACGCTCGTGGAGAAGCCAGAGGCATGGCGAGCAGAGCTCGAATCGCCGCCTACAAGATCTGCTGGGAACTTGGTTGTTACGACTCCGACATACTCGCCGCTATGGATCAGGCGATTGCCGACGGGGTCCACATCATCTCTCTCTCCGTCGGATCCTCCGGCCGTGCCCCTGCGTACTATCGAGATTCCATCGCAATCGGAGCGTTCGGCGCAATGCAGCACGGCGTTGTCGTCTCTTGCTCAGCCGGAAACTCCGGTCCCGGTCCCTACACGGCGGTGAACATCGCTCCATGGATCCTCACCGTCGGCGCCTCCACAATCGACAGAGAGTTTCTCGCAGATGTGATTCTTGGAGACGGTAGGGTTTTCGGCGGCGTATCCCTCTATTCCGGCGATCCTCTCGGCGATTCCAAGCTTCCGTTGGTCTACGGTGGAGATTGCGGAAGCAGGTATTGTTATTACGGAAGCCTCAATTCATCGAAAGTCGCCGGAAAGATCGTAGTATGCGATCGAGGAGGGAACGCCAGAGTCGAAAAAGGAGGCGCCGTGAAATTAGCCGGCGGTCGTGGTATGATTCTCGCCAACACGGAAGAAAACGGCGAAGAACTCTTGGCGGATGCTCATCTTATACCGGGAACAATGGTGGGCGAAATCGCCGGTAACAAACTCAGAGACTACGTCCATTCAGATCCGAATCCAACTGCAACCCTCGTTTTCCGCGGAACCGTGATCGGCGATTCACCACCGGCGCCCAGAGTCGCCGCCTTCTCCAGCCGCGGTCCGAACTATCGAACGGCAGAGATATTGAAGCCGGACGTGATAGCGCCGGGGGTTAACATTCTCGCCGGCTGGACCGGGTTCAGCAGCCCGACCGGTCTGAGCATCGATCCTCGAAGAGTGGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCACGTCAGCGGCGTCGCCGCCTTGCTCCGAAAGGCTTTTCCGGCATGGTCGCCGGCGGCGATCAAATCGGCTCTGATCACCACTTCTTACTCTGTGGACACTTCCGGCAGCCCAATCAAAGATCTCGCCACGAGTGAAGATTCAAATCCGTTCGTCCACGGTGCCGGCCATATCGATCCCAACAGAGCTCTGAATCCCGGTTTGATTTACGACCTCAATTCTCAGGATTACGTATCATTTCTCTGCTCCATTGGCTACGATCCTCGACAAATAGCTGTTTTCGTGAAGGATTCTTCATACTCGCAACTTTGTTCGCACAAATTCACCAATCCCGGAAATCTGAACTACCCATCATTCGCCGTCGTGTTCGACTCCGACGCCGAGGAGGTGGTGAAATACACAAGAACTGTCACGAACGTCGGAAGCGAAGCCGATGCAGTTTATGAAGTGAAAGTAGAATCCCCACAAGGGGTACTAATCAGCGTTCTTCCAAACAAACTGGAGTTCAACGCGAAGAAGACGACACAGTCTTACGAGATTACATTCACCAAAATCAATGGATTCAAGAAATCGGCGAGCTTCGGATCAATTGAATGGAGCGATGGAAGTCACGTGGTGAGGAGTCCGATTGCTGTGTCGTTCAACAGCGGATCTGTAGCTTCGATG

Coding sequence (CDS)

ATGGCGAACTTCCATGTCTGTTCTTTCCTTATCTTCTTCTTCCTCCTCTGTTTCGTTCCGGTGATTTTCTCCCGTTTGCCGGACGATCAGCAGACCTTCATCGTTCACGTTTCTAAATCGGAGAAGCCTGCTCTCTTCTCCTCTCATCATCATTGGCACTCCACCATTCTCGAATCGCTCTCTCCTTCCCTTCATCCAACGAAGCTTCTTTACAACTACGAACGCGCCGTCAACGGCTTCTCCGCCCGCCTCACGGCTGCTCAGGCGGGGGAGCTGCGTCGCGTTCCTGGTGTTGTTTCGGTGATTCCTGATCGGATCCGTCAGCTTCATACGACTCGAACGCCTCATTTCTTAGGTCTCGCTGATAATCTAGGGCTCTGGCCCGACACGAACTATGCGGATGATGTGATTATTGGCGTTCTTGACACCGGAATTTGGCCCGAACGGCCCAGTTTCTCGGACGAGGGCCTTTCTCCGGTTCCGTCAAGCTGGAAAGGCATCTGCCAGACCGGGGAGGGGTTTCCGGCTTCTGCCTGTAATCGGAAGATTATCGGCGCGAGGGCGTACTTCTATGGATACGCGTCTCATTTAGGAAAATCGTTTGAAGATTCTTCGGATTTGAGATCTCCTAGGGATACGGAGGGCCATGGAACTCACACAGCTTCTACGGCCGCAGGGTCGTTTGTTAAGAACGCGAGTTTTCTTGAATACGCTCGTGGAGAAGCCAGAGGCATGGCGAGCAGAGCTCGAATCGCCGCCTACAAGATCTGCTGGGAACTTGGTTGTTACGACTCCGACATACTCGCCGCTATGGATCAGGCGATTGCCGACGGGGTCCACATCATCTCTCTCTCCGTCGGATCCTCCGGCCGTGCCCCTGCGTACTATCGAGATTCCATCGCAATCGGAGCGTTCGGCGCAATGCAGCACGGCGTTGTCGTCTCTTGCTCAGCCGGAAACTCCGGTCCCGGTCCCTACACGGCGGTGAACATCGCTCCATGGATCCTCACCGTCGGCGCCTCCACAATCGACAGAGAGTTTCTCGCAGATGTGATTCTTGGAGACGGTAGGGTTTTCGGCGGCGTATCCCTCTATTCCGGCGATCCTCTCGGCGATTCCAAGCTTCCGTTGGTCTACGGTGGAGATTGCGGAAGCAGGTATTGTTATTACGGAAGCCTCAATTCATCGAAAGTCGCCGGAAAGATCGTAGTATGCGATCGAGGAGGGAACGCCAGAGTCGAAAAAGGAGGCGCCGTGAAATTAGCCGGCGGTCGTGGTATGATTCTCGCCAACACGGAAGAAAACGGCGAAGAACTCTTGGCGGATGCTCATCTTATACCGGGAACAATGGTGGGCGAAATCGCCGGTAACAAACTCAGAGACTACGTCCATTCAGATCCGAATCCAACTGCAACCCTCGTTTTCCGCGGAACCGTGATCGGCGATTCACCACCGGCGCCCAGAGTCGCCGCCTTCTCCAGCCGCGGTCCGAACTATCGAACGGCAGAGATATTGAAGCCGGACGTGATAGCGCCGGGGGTTAACATTCTCGCCGGCTGGACCGGGTTCAGCAGCCCGACCGGTCTGAGCATCGATCCTCGAAGAGTGGAGTTCAATATCATCTCCGGAACTTCAATGTCTTGCCCTCACGTCAGCGGCGTCGCCGCCTTGCTCCGAAAGGCTTTTCCGGCATGGTCGCCGGCGGCGATCAAATCGGCTCTGATCACCACTTCTTACTCTGTGGACACTTCCGGCAGCCCAATCAAAGATCTCGCCACGAGTGAAGATTCAAATCCGTTCGTCCACGGTGCCGGCCATATCGATCCCAACAGAGCTCTGAATCCCGGTTTGATTTACGACCTCAATTCTCAGGATTACGTATCATTTCTCTGCTCCATTGGCTACGATCCTCGACAAATAGCTGTTTTCGTGAAGGATTCTTCATACTCGCAACTTTGTTCGCACAAATTCACCAATCCCGGAAATCTGAACTACCCATCATTCGCCGTCGTGTTCGACTCCGACGCCGAGGAGGTGGTGAAATACACAAGAACTGTCACGAACGTCGGAAGCGAAGCCGATGCAGTTTATGAAGTGAAAGTAGAATCCCCACAAGGGGTACTAATCAGCGTTCTTCCAAACAAACTGGAGTTCAACGCGAAGAAGACGACACAGTCTTACGAGATTACATTCACCAAAATCAATGGATTCAAGAAATCGGCGAGCTTCGGATCAATTGAATGGAGCGATGGAAGTCACGTGGTGAGGAGTCCGATTGCTGTGTCGTTCAACAGCGGATCTGTAGCTTCGATG

Protein sequence

MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNPGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM
Homology
BLAST of MS009601 vs. NCBI nr
Match: XP_038897980.1 (subtilisin-like protease SBT1.4 [Benincasa hispida])

HSP 1 Score: 1389.4 bits (3595), Expect = 0.0e+00
Identity = 682/771 (88.46%), Postives = 728/771 (94.42%), Query Frame = 0

Query: 1   MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
           MA  H+CS L F F+LCF PVIFSR P++Q+TFIVHVSKSEKP+LFSSHHHWHS+ILESL
Sbjct: 1   MAKSHLCSSLFFLFILCFAPVIFSRSPENQETFIVHVSKSEKPSLFSSHHHWHSSILESL 60

Query: 61  SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
           SPS HPTKLLYNYERAVNGFSARLTAAQA +LR VPGV+SVIPDRIRQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYERAVNGFSARLTAAQAAQLRHVPGVISVIPDRIRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
           ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+SWKG C+TGEGF AS CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCETGEGFSASFCN 180

Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
           RKIIGARAYFYGYAS+ G+SF  SSD +S RDTEGHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAYFYGYASNFGRSFNGSSDFKSARDTEGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GC+DSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCFDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
           GVSLYSGDPLGDSK+PLVYGGDCGSRYCY GSLN SKVAGKIVVCDRGGNARV KGGAVK
Sbjct: 361 GVSLYSGDPLGDSKVPLVYGGDCGSRYCYSGSLNPSKVAGKIVVCDRGGNARVAKGGAVK 420

Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
            AGG GM+LANTEENGEELLAD+HLIPGTMVGEIAGNKLRDY+HSDPNPTA +VFRGTVI
Sbjct: 421 SAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHSDPNPTAIIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILA W+G+SSPTGL+IDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILASWSGYSSPTGLNIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFP WSPAAIKSALITTSYS+D+SGSPIKDL+TSE+SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLSTSEESNPF 600

Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
           VHGAGHIDPN+ALNPGLIYD++ QDYVSFLCSIGYD +QIAVFVKDSSYSQLC HK +NP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDISPQDYVSFLCSIGYDSKQIAVFVKDSSYSQLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
           GNLNYPSFAVVF  + E+VVKYTRTVTNVG E+D VYEVKVE+PQGV ISV+PNKL FNA
Sbjct: 661 GNLNYPSFAVVF--NGEDVVKYTRTVTNVGDESDVVYEVKVEAPQGVEISVVPNKLSFNA 720

Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           +KTT SYEITFTKI+GF ++ASFGSI+WSDGSH VRSPIAVSFN+GS+ASM
Sbjct: 721 EKTTDSYEITFTKISGFVEAASFGSIQWSDGSHNVRSPIAVSFNTGSIASM 769

BLAST of MS009601 vs. NCBI nr
Match: XP_022992130.1 (subtilisin-like protease SBT1.4 [Cucurbita maxima])

HSP 1 Score: 1368.2 bits (3540), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 719/771 (93.26%), Query Frame = 0

Query: 1   MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
           MANFH CS + FFF LCF+PVIFSR PD Q+TFIVHVSKSEKP+LFSSH+ WHS+IL+SL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
           SPS  P KLLYNYERAVNGF+AR+TA QA ELRRVPGVV VIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
           ADNLGLWPDTNYADDVIIGVLDTGIWPE PSFSDEGLSPVP+SWKG C+ GEGF A+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
           RKIIGARA+F+GY S++G+     SD +S RD++GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
           GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG AVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
            AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSGSPIKDL+TS DSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600

Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
           VHG GHIDPN+ALNPGLIYDLN  DYVSFLCSIGYD RQIAVFVKDSSYS LC HK +NP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
           GNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           KKTT+SYEITFTKI+GFK SASFGSI+WSDGSH+VRSPIAVSFN+ S+ASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of MS009601 vs. NCBI nr
Match: KAG6575439.1 (Subtilisin-like protease 1.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1364.4 bits (3530), Expect = 0.0e+00
Identity = 671/773 (86.80%), Postives = 718/773 (92.88%), Query Frame = 0

Query: 1   MANFHVCS--FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILE 60
           MANFH CS  F  FFF LCF+PVIFSR PD Q+TFIVHVSK EKP+LFSSH+ WHS+IL+
Sbjct: 1   MANFHRCSSVFFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILD 60

Query: 61  SLSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFL 120
           SLSPS  P KLLYNYERAVNGF+AR+T AQA ELRRVPGVVSVIPDR+RQLHTTRTPHFL
Sbjct: 61  SLSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFL 120

Query: 121 GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASA 180
           GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+SWKG C+ GEGF A+ 
Sbjct: 121 GLADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATT 180

Query: 181 CNRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYA 240
           CNRKIIGARA+F+GY S++G+     SD +S RD++GHGTHTASTAAGSFVKNASF +YA
Sbjct: 181 CNRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYA 240

Query: 241 RGEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD 300
           RGEARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD
Sbjct: 241 RGEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360
           SIAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV
Sbjct: 301 SIAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRV 360

Query: 361 FGGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGA 420
           F GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG A
Sbjct: 361 FSGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSA 420

Query: 421 VKLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGT 480
           VK AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGT
Sbjct: 421 VKSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGT 480

Query: 481 VIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEF 540
           VI DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEF
Sbjct: 481 VISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSN 600
           NIISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSG PIKDL+TS DSN
Sbjct: 541 NIISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSN 600

Query: 601 PFVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFT 660
           PFVHG GHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDSSYS LC HK +
Sbjct: 601 PFVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLS 660

Query: 661 NPGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEF 720
           NPGNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF
Sbjct: 661 NPGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEF 720

Query: 721 NAKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           +AKKTTQSYEITFTKI+GFK SASFGSI+WSDGSH VRSPIAVSFN+ S+ASM
Sbjct: 721 SAKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHTVRSPIAVSFNTASIASM 773

BLAST of MS009601 vs. NCBI nr
Match: XP_022964269.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])

HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 716/771 (92.87%), Query Frame = 0

Query: 1   MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
           MAN H+CS   FF +LCFVPVIFSR   +Q+TFIVHVSKSEKPALFSSHHHWHS+ILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
           SPS HPTKLLY+YE A NGFSAR+TAAQA ELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
           AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVP++WKG CQTG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
           RKIIGARAYF GY S + +S + SSD +S RDT+GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF 
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
           GVSLYSGDPLGDSKLPLVY GDCG RYCY G+L+SSKVAGKIVVCDRGGNARV KG AVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
            AGG GMILANTEENGEELLAD+HLIPGTM+GEIAGN L+DY+HSDP+PTAT+VFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G+SSPT LSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFP WSPAAIKSAL+TTSYS+D+SG PI DL+TS+ SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
           VHGAGHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDS Y+QLC HK T+P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
           GNLNYPSFAVVFDSD +EVVKYTRTVTNVG E DAVYEVKVESPQGV I+VLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           K  TQSYEITFTKING K SASFGSI+WSDGSH+VRSP+AVSFNSG +ASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of MS009601 vs. NCBI nr
Match: XP_022953749.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 669/772 (86.66%), Postives = 718/772 (93.01%), Query Frame = 0

Query: 1   MANFHVC-SFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILES 60
           MANFH C S   FFF LCF+PVIFSR PD Q+TFIVHVSK EKP+LFSSH+ WHS+IL+S
Sbjct: 1   MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLG 120
           LSPS  P KLLYNYERAVNGF+AR+T AQA ELRRVPGVVSVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASAC 180
           LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+SWKG C+ GEGF A+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYAR 240
           NRKIIGARA+F+GY S++G+     SD +S RD++GHGTHTASTAAGSFVKNASF +YAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 GGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 420
            GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTV 480
           K AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFN 540
           I DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNP 600
           IISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSG PIKDL+TS DSNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600

Query: 601 FVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTN 660
           FVHG GHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDSSYS LC HK +N
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFN 720
           PGNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF+
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 AKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           AKKTTQSYEITFTKI+GFK SASFGSI+WSDGSH+VRSPIAVSFN+ S+ASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772

BLAST of MS009601 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 3.4e-290
Identity = 501/769 (65.15%), Postives = 596/769 (77.50%), Query Frame = 0

Query: 9   FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTK 68
           F +F  LLCF     S   D  +++IVHV +S KP+LFSSH++WH ++L SL  S  P  
Sbjct: 10  FFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPAT 69

Query: 69  LLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWP 128
           LLY+Y RAV+GFSARL+  Q   LRR P V+SVIPD+ R++HTT TP FLG + N GLW 
Sbjct: 70  LLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWS 129

Query: 129 DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARA 188
           ++NY +DVI+GVLDTGIWPE PSFSD GL P+PS+WKG C+ G  FPAS+CNRK+IGARA
Sbjct: 130 NSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARA 189

Query: 189 YFYGYASHL-GKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMAS 248
           ++ GY +   G     + + RSPRDTEGHGTHTASTAAGS V NAS  +YARG A GMAS
Sbjct: 190 FYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMAS 249

Query: 249 RARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 308
           +ARIAAYKICW  GCYDSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGAFGA
Sbjct: 250 KARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGA 309

Query: 309 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSG 368
            +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+VF G SLY+G
Sbjct: 310 TRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAG 369

Query: 369 DPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGM 428
           + L DS+L LVY GDCGSR CY G LNSS V GKIV+CDRGGNARVEKG AVKLAGG GM
Sbjct: 370 ESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGM 429

Query: 429 ILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAP 488
           ILANT E+GEEL AD+HL+P TMVG  AG+++RDY+ +  +PTA + F GT+IG SPP+P
Sbjct: 430 ILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSP 489

Query: 489 RVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMS 548
           RVAAFSSRGPN+ T  ILKPDVIAPGVNILAGWTG   PT L IDPRRV+FNIISGTSMS
Sbjct: 490 RVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMS 549

Query: 549 CPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHI 608
           CPHVSG+AALLRKA P WSPAAIKSAL+TT+Y V+ SG PI+DLAT + SN F+HGAGH+
Sbjct: 550 CPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHV 609

Query: 609 DPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLC-SHKFTNPGNLNYP 668
           DPN+ALNPGL+YD+  ++YV+FLC++GY+   I VF++D +    C + K    G+LNYP
Sbjct: 610 DPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYP 669

Query: 669 SFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQS 728
           SF+VVF S   EVVKY R V NVGS  DAVYEV V+SP  V I V P+KL F+ +K+   
Sbjct: 670 SFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLE 729

Query: 729 YEITFTKI-----NGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVAS 771
           YE+TF  +      G      FGSIEW+DG HVV+SP+AV +  GSV S
Sbjct: 730 YEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of MS009601 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 3.1e-227
Identity = 424/761 (55.72%), Postives = 527/761 (69.25%), Query Frame = 0

Query: 12  FFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLY 71
           FF LLC      S    DQ T+IVH++KS+ P+ F  H +W+ + L S+S S    +LLY
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---AELLY 70

Query: 72  NYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLG-LWPDT 131
            YE A++GFS RLT  +A  L   PGV+SV+P+   +LHTTRTP FLGL ++   L+P+ 
Sbjct: 71  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 130

Query: 132 NYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYF 191
               DV++GVLDTG+WPE  S+SDEG  P+PSSWKG C+ G  F AS CNRK+IGAR + 
Sbjct: 131 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 190

Query: 192 YGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRAR 251
            GY S +G   ++S + RSPRD +GHGTHT+STAAGS V+ AS L YA G ARGMA RAR
Sbjct: 191 RGYESTMG-PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRAR 250

Query: 252 IAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQH 311
           +A YK+CW  GC+ SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF AM+ 
Sbjct: 251 VAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAAMER 310

Query: 312 GVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPL 371
           G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F GVSL+ G+ L
Sbjct: 311 GILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL 370

Query: 372 GDSKLPLVYGGDCGS----RYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRG 431
            D  LP +Y G+  +      C  G+L   KV GKIV+CDRG NARV+KG  VK AGG G
Sbjct: 371 PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVG 430

Query: 432 MILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPA 491
           MILANT  NGEEL+ADAHL+P T VGE AG+ +R YV +DPNPTA++   GTV+G   P+
Sbjct: 431 MILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG-VKPS 490

Query: 492 PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSM 551
           P VAAFSSRGPN  T  ILKPD+IAPGVNILA WTG + PTGL+ D RRVEFNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550

Query: 552 SCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGH 611
           SCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D+AT + S PF HGAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610

Query: 612 IDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCS-HKFTNPGNLNY 671
           + P  A NPGLIYDL ++DY+ FLC++ Y   QI      S  +  C   K  +  +LNY
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVADLNY 670

Query: 672 PSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVES-PQGVLISVLPNKLEFNAKKTT 731
           PSFAV  + D     KYTRTVT+VG      Y VKV S   GV ISV P  L F      
Sbjct: 671 PSFAV--NVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKEANEK 730

Query: 732 QSYEITFTKINGFKKSA--SFGSIEWSDGSHVVRSPIAVSF 764
           +SY +TFT ++  K S   SFGSIEWSDG HVV SP+A+S+
Sbjct: 731 KSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of MS009601 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 2.6e-218
Identity = 398/740 (53.78%), Postives = 512/740 (69.19%), Query Frame = 0

Query: 30  QQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLYNYERAVNGFSARLTAAQA 89
           ++T+I+ V+ S+KP  F +HH W+++ L S S       LLY Y  + +GFSA L + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  GE-LRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWPDTNYADDVIIGVLDTGIWPE 149
              L     ++ +  D +  LHTTRTP FLGL    G+    + ++ VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYFYGYASHLGKSFEDSSDLR 209
             SF D  +  +PS WKG C++G  F +  CN+K+IGAR++  G+    G  F    +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRARIAAYKICWELGCYDSDIL 269
           SPRD +GHGTHT++TAAGS V+NASFL YA G ARGMA+RAR+A YK+CW  GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPLGDSKLPLVY--GGDCGSR 389
            N+APW++TVGA T+DR+F A   LG+G+   GVSLYSG  +G   L LVY  G    S 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGMILANTEENGEELLADAHLI 449
            C  GSL+SS V GKIVVCDRG NARVEKG  V+ AGG GMI+ANT  +GEEL+AD+HL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILK 509
           P   VG+  G+ LR+YV SD  PTA LVF+GTV+ D  P+P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPAWS 569
           PDVI PGVNILAGW+    PTGL  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDY 629
           P+AIKSAL+TT+Y +D + +P+ D A +  SNP+ HG+GH+DP +AL+PGL+YD+++++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNPGNLNYPSFAVVFDSDAEEVVKYTRTV 689
           + FLCS+ Y    I   VK  S +  CS KF++PG LNYPSF+V+F    + VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686

Query: 690 TNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQSYEITFTKING--FKKSASFG 749
           TNVG+ A +VY+V V     V ISV P+KL F +    + Y +TF    G      A FG
Sbjct: 687 TNVGA-ASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 750 SIEWSDGSHVVRSPIAVSFN 765
           SI WS+  H VRSP+A S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of MS009601 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 8.4e-209
Identity = 389/778 (50.00%), Postives = 515/778 (66.20%), Query Frame = 0

Query: 9   FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTK 68
           F  FFFLL       S    +  T+IVHV    KP++F +H HW+++ L SL+ S  P  
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPS 63

Query: 69  LLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL--ADNLGL 128
           +++ Y+   +GFSARLT+  A +L   P V+SVIP+++R LHTTR+P FLGL   D  GL
Sbjct: 64  IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGL 123

Query: 129 WPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGA 188
             ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C   + FP SACNRK++GA
Sbjct: 124 LEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGA 183

Query: 189 RAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMA 248
           R +  GY +  GK   ++++ RSPRD++GHGTHTAS +AG +V  AS L YA G A GMA
Sbjct: 184 RFFCGGYEATNGK-MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 243

Query: 249 SRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFG 308
            +AR+AAYK+CW  GCYDSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFG
Sbjct: 244 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFG 303

Query: 309 AMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYS 368
           A+  G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++  GVS+Y 
Sbjct: 304 AIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYG 363

Query: 369 GDPLGDSKL-PLVYGGDC------GSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 428
           G  L   ++ PLVYGG         S  C  GSL+ + V GKIV+CDRG N+R  KG  V
Sbjct: 364 GPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIV 423

Query: 429 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYV------HSDPNPTATL 488
           +  GG GMI+AN   +GE L+AD H++P T VG   G+++R Y+       S  +PTAT+
Sbjct: 424 RKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATI 483

Query: 489 VFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDP 548
           VF+GT +G   PAP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+G++ D 
Sbjct: 484 VFKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 543

Query: 549 RRVEFNIISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLAT 608
           RR EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y+VD SG P+ D +T
Sbjct: 544 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDEST 603

Query: 609 SEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVK---DSSYS 668
              S+   +G+GH+ P +A++PGL+YD+ S DY++FLC+  Y    I    +   D   +
Sbjct: 604 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 663

Query: 669 QLCSHKFTNPGNLNYPSFAVVFDSDAEEVVK--YTRTVTNVGSEADAVYEVKVESPQGVL 728
           +   H     GNLNYPSF+VVF    E  +   + RTVTNVG ++D+VYE+K+  P+G  
Sbjct: 664 RRAGH----VGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVG-DSDSVYEIKIRPPRGTT 723

Query: 729 ISVLPNKLEFNAKKTTQSYEI----TFTKINGFKKSASFGSIEWSDGSHVVRSPIAVS 763
           ++V P KL F       S+ +    T  K++    +   G I WSDG   V SP+ V+
Sbjct: 724 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of MS009601 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 2.0e-202
Identity = 377/769 (49.02%), Postives = 496/769 (64.50%), Query Frame = 0

Query: 6   VCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLH 65
           + S  I   L    P I        +TFI  +     P++F +H+HW+ST     S    
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES---- 60

Query: 66  PTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLG 125
             ++++ Y    +GFSA +T  +A  LR  P V++V  DR R+LHTTR+P FLGL +  G
Sbjct: 61  --RIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG 120

Query: 126 LWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIG 185
           LW +++Y  DVIIGV DTGIWPER SFSD  L P+P  W+G+C++G  F    CNRKIIG
Sbjct: 121 LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIG 180

Query: 186 ARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGM 245
           AR +  G  + +      + +  SPRD +GHGTHT+STAAG     AS   YA G A+G+
Sbjct: 181 ARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGV 240

Query: 246 ASRARIAAYKICW-ELGCYDSDILAAMDQAIADGVHIISLSV-GSSGRAPAYYRDSIAIG 305
           A +ARIAAYK+CW + GC DSDILAA D A+ DGV +IS+S+ G  G    YY D IAIG
Sbjct: 241 APKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIG 300

Query: 306 AFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVS 365
           ++GA   G+ VS SAGN GP   +  N+APW+ TVGASTIDR F AD ILGDG    GVS
Sbjct: 301 SYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVS 360

Query: 366 LYSGDPLGDSKLPLVYGGDCG---SRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 425
           LY+G PL     P+VY G  G   +  C   +L+  +V GKIV+CDRG + RV KG  VK
Sbjct: 361 LYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 420

Query: 426 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 485
            AGG GMILAN   NGE L+ DAHLIP   VG   G++++ Y  S PNP A++ FRGT++
Sbjct: 421 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 480

Query: 486 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 545
           G   PAP +A+FS RGPN  + EILKPD+IAPGVNILA WT    PTGL  DPR+ EFNI
Sbjct: 481 G-IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNI 540

Query: 546 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 605
           +SGTSM+CPHVSG AALL+ A P WSPA I+SA++TT+  VD S   + D +T + + P+
Sbjct: 541 LSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPY 600

Query: 606 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 665
            +G+GH++  RA+NPGL+YD+ + DY++FLCSIGY P+ I V  +  +  +  + +  +P
Sbjct: 601 DYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPSP 660

Query: 666 GNLNYPSFAVVFDSDAEEVVKYT--RTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEF 725
           GNLNYPS   VF ++   +V  T  RT TNVG +A+AVY  ++ESP+GV ++V P +L F
Sbjct: 661 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLVF 720

Query: 726 NAKKTTQSYEITFT----KINGFKKSASFGSIEWSD-GSHVVRSPIAVS 763
            +    +SY +T T     +   +  A FGS+ W D G HVVRSPI V+
Sbjct: 721 TSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

BLAST of MS009601 vs. ExPASy TrEMBL
Match: A0A6J1JUV1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3 SV=1)

HSP 1 Score: 1368.2 bits (3540), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 719/771 (93.26%), Query Frame = 0

Query: 1   MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
           MANFH CS + FFF LCF+PVIFSR PD Q+TFIVHVSKSEKP+LFSSH+ WHS+IL+SL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
           SPS  P KLLYNYERAVNGF+AR+TA QA ELRRVPGVV VIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
           ADNLGLWPDTNYADDVIIGVLDTGIWPE PSFSDEGLSPVP+SWKG C+ GEGF A+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
           RKIIGARA+F+GY S++G+     SD +S RD++GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
           GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG AVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
            AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSGSPIKDL+TS DSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600

Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
           VHG GHIDPN+ALNPGLIYDLN  DYVSFLCSIGYD RQIAVFVKDSSYS LC HK +NP
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
           GNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           KKTT+SYEITFTKI+GFK SASFGSI+WSDGSH+VRSPIAVSFN+ S+ASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of MS009601 vs. ExPASy TrEMBL
Match: A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)

HSP 1 Score: 1364.0 bits (3529), Expect = 0.0e+00
Identity = 670/771 (86.90%), Postives = 716/771 (92.87%), Query Frame = 0

Query: 1   MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
           MAN H+CS   FF +LCFVPVIFSR   +Q+TFIVHVSKSEKPALFSSHHHWHS+ILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
           SPS HPTKLLY+YE A NGFSAR+TAAQA ELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
           AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVP++WKG CQTG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
           RKIIGARAYF GY S + +S + SSD +S RDT+GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF 
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
           GVSLYSGDPLGDSKLPLVY GDCG RYCY G+L+SSKVAGKIVVCDRGGNARV KG AVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
            AGG GMILANTEENGEELLAD+HLIPGTM+GEIAGN L+DY+HSDP+PTAT+VFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G+SSPT LSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFP WSPAAIKSAL+TTSYS+D+SG PI DL+TS+ SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
           VHGAGHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDS Y+QLC HK T+P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
           GNLNYPSFAVVFDSD +EVVKYTRTVTNVG E DAVYEVKVESPQGV I+VLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           K  TQSYEITFTKING K SASFGSI+WSDGSH+VRSP+AVSFNSG +ASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of MS009601 vs. ExPASy TrEMBL
Match: A0A6J1GQK5 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE=3 SV=1)

HSP 1 Score: 1362.8 bits (3526), Expect = 0.0e+00
Identity = 669/772 (86.66%), Postives = 718/772 (93.01%), Query Frame = 0

Query: 1   MANFHVC-SFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILES 60
           MANFH C S   FFF LCF+PVIFSR PD Q+TFIVHVSK EKP+LFSSH+ WHS+IL+S
Sbjct: 1   MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLG 120
           LSPS  P KLLYNYERAVNGF+AR+T AQA ELRRVPGVVSVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASAC 180
           LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+SWKG C+ GEGF A+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYAR 240
           NRKIIGARA+F+GY S++G+     SD +S RD++GHGTHTASTAAGSFVKNASF +YAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 GGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 420
            GVSLYSGDPLGDSKLPLVYGGDCG+RYCY GSL+ SKVAGKIVVCDRGGNARV KG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTV 480
           K AGG G+ILANTEENGEELLAD+HLIPGTM+GEIAGNKLRDY+HSDPNP+AT+VFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFN 540
           I DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G++SP+ LSID RRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNP 600
           IISGTSMSCPHVSGVAALLRKAFP W+PAAIKSALITTSYS+DTSG PIKDL+TS DSNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600

Query: 601 FVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTN 660
           FVHG GHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDSSYS LC HK +N
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFN 720
           PGNLNYPSFAVVF S+ EEVVKYTRTVTNVG EADAVYEVKVE+PQGV ISVLPNKLEF+
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 AKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           AKKTTQSYEITFTKI+GFK SASFGSI+WSDGSH+VRSPIAVSFN+ S+ASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772

BLAST of MS009601 vs. ExPASy TrEMBL
Match: A0A6J1KIR1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111494328 PE=3 SV=1)

HSP 1 Score: 1360.9 bits (3521), Expect = 0.0e+00
Identity = 665/771 (86.25%), Postives = 717/771 (93.00%), Query Frame = 0

Query: 1   MANFHVCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESL 60
           MAN H+CS + FF +LCFVPVIFSR   +Q+TFIVHVSKSEKPALFSSHHHWHS+IL+SL
Sbjct: 1   MANLHLCSSVFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDSL 60

Query: 61  SPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL 120
           SPS HPTKLLYNYE A NGFSAR+TAAQA ELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACN 180
           AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVP++WKG CQTG GF ASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARG 240
           RKIIGARAYF GY S + +S + SSD +S RDT+GHGTHTASTAAGSFVKNASF +YARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300
           EARGMA+RARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFG 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF 
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 420
           GVSLYSGDPLGDSKLPLVY GDCG+RYCY G+L+SSKVAGKIVVCDRGGNARV KG  VK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTVK 420

Query: 421 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 480
            AGG GMILANTEENGEELLAD+HLIPGTM+GEIAGN L+DY+HSDP+PTAT+VFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPN+RTAEILKPDVIAPGVNILAGW+G+SSPT LSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 600
           ISGTSMSCPHVSGVAALLRKAFP WSPAAIKSAL+TTSYS+D+SG PI DL+TS+ SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 660
           VHGAGHIDPN+ALNPGLIYDLN QDYVSFLCSIGYD RQIAVFVKDS Y++LC HK T+P
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNA 720
           GNLNYPSFAVVFDSD +EVVKYTRTVTNVG E DAVYEVKVESP+GV I+VLPNKLEF+A
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFSA 720

Query: 721 KKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVASM 772
           K  TQSYEITFTKING K SASFGSI+WSDGSH+VRSP+AVSFNSG +ASM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of MS009601 vs. ExPASy TrEMBL
Match: A0A0A0K6Z1 (Subtilase family protein OS=Cucumis sativus OX=3659 GN=Csa_7G448910 PE=3 SV=1)

HSP 1 Score: 1341.6 bits (3471), Expect = 0.0e+00
Identity = 667/773 (86.29%), Postives = 712/773 (92.11%), Query Frame = 0

Query: 1   MANFHV-CSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILES 60
           MANFH+  S   FFFL CF P+IFSR  ++Q+T+IVHVSKSEKP+LFSSHHHWHS+ILES
Sbjct: 1   MANFHLPSSSFFFFFLFCFFPLIFSRSLENQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSLHPTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLG 120
           LS S HPTKLLYNYERA NGFSAR+T  QA ELRRVPG++SVIPD+IRQLHTTRTPHFLG
Sbjct: 61  LSSSPHPTKLLYNYERAANGFSARITTVQAEELRRVPGIISVIPDQIRQLHTTRTPHFLG 120

Query: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASAC 180
           LADNLGLW DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVP+ WKG C TGEG  A AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPARWKGTCDTGEGVSAFAC 180

Query: 181 NRKIIGARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYAR 240
           NRKIIGARAYFYGY S+L  S + SSD +S RDTEGHGTHTASTAAGSFV NASF +YAR
Sbjct: 181 NRKIIGARAYFYGYESNLRGSLKVSSDFKSARDTEGHGTHTASTAAGSFVNNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300
           GEARGMASRARIAAYKICWE GCYDSDILAAMDQAI+DGV +ISLSVGSSGRAPAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 GGVSLYSGDPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 420
            GVSLYSGDPLGDSKL LVYGGDCGSRYCY GSL+SSKVAGKIVVCDRGGNARV KGGAV
Sbjct: 361 SGVSLYSGDPLGDSKLQLVYGGDCGSRYCYSGSLDSSKVAGKIVVCDRGGNARVAKGGAV 420

Query: 421 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTV 480
           K AGG GM+LANTEENGEELLAD+HLIPGTMVG IAGNKLRDY+H+DPNPTAT+VFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGAIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFN 540
           IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGW+G+SSPTGL+IDPRRVEFN
Sbjct: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNP 600
           IISGTSMSCPHVSGVAALLRKAFP WSPAAIKSALITTSYS+D+SG PIKDL+TSE+SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGKPIKDLSTSEESNP 600

Query: 601 FVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTN 660
           FVHGAGHI+PN+ALNPGLIYDL  QDYVSFLCSIGYD +QIAVFVK SSY QLC HK TN
Sbjct: 601 FVHGAGHINPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYFQLCEHKLTN 660

Query: 661 PGNLNYPSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFN 720
           PGNLNYPSF+VVFD   EEVVKYTRTVTNVG E + VYEVKVE+PQGV+ISV+PNKLEFN
Sbjct: 661 PGNLNYPSFSVVFDE--EEVVKYTRTVTNVGDETEVVYEVKVEAPQGVVISVVPNKLEFN 720

Query: 721 AKKTTQSYEITFTKINGFKKSASFGSIEWSDGSHVVRSPIAVSFNS-GSVASM 772
            +KTTQSYEITFTKINGFK+SASFGSI+W DG H VRSPIAVSF + GS+ASM
Sbjct: 721 KEKTTQSYEITFTKINGFKESASFGSIQWGDGIHSVRSPIAVSFKTGGSIASM 771

BLAST of MS009601 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 998.8 bits (2581), Expect = 2.4e-291
Identity = 501/769 (65.15%), Postives = 596/769 (77.50%), Query Frame = 0

Query: 9   FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTK 68
           F +F  LLCF     S   D  +++IVHV +S KP+LFSSH++WH ++L SL  S  P  
Sbjct: 10  FFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPAT 69

Query: 69  LLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWP 128
           LLY+Y RAV+GFSARL+  Q   LRR P V+SVIPD+ R++HTT TP FLG + N GLW 
Sbjct: 70  LLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWS 129

Query: 129 DTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARA 188
           ++NY +DVI+GVLDTGIWPE PSFSD GL P+PS+WKG C+ G  FPAS+CNRK+IGARA
Sbjct: 130 NSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARA 189

Query: 189 YFYGYASHL-GKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMAS 248
           ++ GY +   G     + + RSPRDTEGHGTHTASTAAGS V NAS  +YARG A GMAS
Sbjct: 190 FYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMAS 249

Query: 249 RARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGA 308
           +ARIAAYKICW  GCYDSDILAAMDQA+ADGVH+ISLSVG+SG AP Y+ DSIAIGAFGA
Sbjct: 250 KARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGA 309

Query: 309 MQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSG 368
            +HG+VVSCSAGNSGP P TA NIAPWILTVGAST+DREF A+ I GDG+VF G SLY+G
Sbjct: 310 TRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAG 369

Query: 369 DPLGDSKLPLVYGGDCGSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGM 428
           + L DS+L LVY GDCGSR CY G LNSS V GKIV+CDRGGNARVEKG AVKLAGG GM
Sbjct: 370 ESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGM 429

Query: 429 ILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAP 488
           ILANT E+GEEL AD+HL+P TMVG  AG+++RDY+ +  +PTA + F GT+IG SPP+P
Sbjct: 430 ILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSP 489

Query: 489 RVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMS 548
           RVAAFSSRGPN+ T  ILKPDVIAPGVNILAGWTG   PT L IDPRRV+FNIISGTSMS
Sbjct: 490 RVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMS 549

Query: 549 CPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHI 608
           CPHVSG+AALLRKA P WSPAAIKSAL+TT+Y V+ SG PI+DLAT + SN F+HGAGH+
Sbjct: 550 CPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHV 609

Query: 609 DPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLC-SHKFTNPGNLNYP 668
           DPN+ALNPGL+YD+  ++YV+FLC++GY+   I VF++D +    C + K    G+LNYP
Sbjct: 610 DPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYP 669

Query: 669 SFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQS 728
           SF+VVF S   EVVKY R V NVGS  DAVYEV V+SP  V I V P+KL F+ +K+   
Sbjct: 670 SFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLE 729

Query: 729 YEITFTKI-----NGFKKSASFGSIEWSDGSHVVRSPIAVSFNSGSVAS 771
           YE+TF  +      G      FGSIEW+DG HVV+SP+AV +  GSV S
Sbjct: 730 YEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of MS009601 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 789.6 bits (2038), Expect = 2.2e-228
Identity = 424/761 (55.72%), Postives = 527/761 (69.25%), Query Frame = 0

Query: 12  FFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLY 71
           FF LLC      S    DQ T+IVH++KS+ P+ F  H +W+ + L S+S S    +LLY
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---AELLY 70

Query: 72  NYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLG-LWPDT 131
            YE A++GFS RLT  +A  L   PGV+SV+P+   +LHTTRTP FLGL ++   L+P+ 
Sbjct: 71  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 130

Query: 132 NYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYF 191
               DV++GVLDTG+WPE  S+SDEG  P+PSSWKG C+ G  F AS CNRK+IGAR + 
Sbjct: 131 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 190

Query: 192 YGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRAR 251
            GY S +G   ++S + RSPRD +GHGTHT+STAAGS V+ AS L YA G ARGMA RAR
Sbjct: 191 RGYESTMG-PIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRAR 250

Query: 252 IAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQH 311
           +A YK+CW  GC+ SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF AM+ 
Sbjct: 251 VAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFAAMER 310

Query: 312 GVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPL 371
           G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F GVSL+ G+ L
Sbjct: 311 GILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL 370

Query: 372 GDSKLPLVYGGDCGS----RYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRG 431
            D  LP +Y G+  +      C  G+L   KV GKIV+CDRG NARV+KG  VK AGG G
Sbjct: 371 PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVG 430

Query: 432 MILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPA 491
           MILANT  NGEEL+ADAHL+P T VGE AG+ +R YV +DPNPTA++   GTV+G   P+
Sbjct: 431 MILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG-VKPS 490

Query: 492 PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSM 551
           P VAAFSSRGPN  T  ILKPD+IAPGVNILA WTG + PTGL+ D RRVEFNIISGTSM
Sbjct: 491 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 550

Query: 552 SCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGH 611
           SCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G P+ D+AT + S PF HGAGH
Sbjct: 551 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 610

Query: 612 IDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCS-HKFTNPGNLNY 671
           + P  A NPGLIYDL ++DY+ FLC++ Y   QI      S  +  C   K  +  +LNY
Sbjct: 611 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIR---SVSRRNYTCDPSKSYSVADLNY 670

Query: 672 PSFAVVFDSDAEEVVKYTRTVTNVGSEADAVYEVKVES-PQGVLISVLPNKLEFNAKKTT 731
           PSFAV  + D     KYTRTVT+VG      Y VKV S   GV ISV P  L F      
Sbjct: 671 PSFAV--NVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKEANEK 730

Query: 732 QSYEITFTKINGFKKSA--SFGSIEWSDGSHVVRSPIAVSF 764
           +SY +TFT ++  K S   SFGSIEWSDG HVV SP+A+S+
Sbjct: 731 KSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of MS009601 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 760.0 bits (1961), Expect = 1.9e-219
Identity = 398/740 (53.78%), Postives = 512/740 (69.19%), Query Frame = 0

Query: 30  QQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTKLLYNYERAVNGFSARLTAAQA 89
           ++T+I+ V+ S+KP  F +HH W+++ L S S       LLY Y  + +GFSA L + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  GE-LRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLGLWPDTNYADDVIIGVLDTGIWPE 149
              L     ++ +  D +  LHTTRTP FLGL    G+    + ++ VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGARAYFYGYASHLGKSFEDSSDLR 209
             SF D  +  +PS WKG C++G  F +  CN+K+IGAR++  G+    G  F    +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMASRARIAAYKICWELGCYDSDIL 269
           SPRD +GHGTHT++TAAGS V+NASFL YA G ARGMA+RAR+A YK+CW  GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G  G AP YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLG-GGSAP-YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYSGDPLGDSKLPLVY--GGDCGSR 389
            N+APW++TVGA T+DR+F A   LG+G+   GVSLYSG  +G   L LVY  G    S 
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVKLAGGRGMILANTEENGEELLADAHLI 449
            C  GSL+SS V GKIVVCDRG NARVEKG  V+ AGG GMI+ANT  +GEEL+AD+HL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILK 509
           P   VG+  G+ LR+YV SD  PTA LVF+GTV+ D  P+P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPAWS 569
           PDVI PGVNILAGW+    PTGL  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALITTSYSVDTSGSPIKDLATSEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDY 629
           P+AIKSAL+TT+Y +D + +P+ D A +  SNP+ HG+GH+DP +AL+PGL+YD+++++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNPGNLNYPSFAVVFDSDAEEVVKYTRTV 689
           + FLCS+ Y    I   VK  S +  CS KF++PG LNYPSF+V+F    + VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRPSVN--CSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686

Query: 690 TNVGSEADAVYEVKVESPQGVLISVLPNKLEFNAKKTTQSYEITFTKING--FKKSASFG 749
           TNVG+ A +VY+V V     V ISV P+KL F +    + Y +TF    G      A FG
Sbjct: 687 TNVGA-ASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 750 SIEWSDGSHVVRSPIAVSFN 765
           SI WS+  H VRSP+A S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of MS009601 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 728.4 bits (1879), Expect = 6.0e-210
Identity = 389/778 (50.00%), Postives = 515/778 (66.20%), Query Frame = 0

Query: 9   FLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLHPTK 68
           F  FFFLL       S    +  T+IVHV    KP++F +H HW+++ L SL+ S  P  
Sbjct: 4   FFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPS 63

Query: 69  LLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGL--ADNLGL 128
           +++ Y+   +GFSARLT+  A +L   P V+SVIP+++R LHTTR+P FLGL   D  GL
Sbjct: 64  IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGL 123

Query: 129 WPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIGA 188
             ++++  D++IGV+DTG+WPERPSF D GL PVP  WKG C   + FP SACNRK++GA
Sbjct: 124 LEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGA 183

Query: 189 RAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGMA 248
           R +  GY +  GK   ++++ RSPRD++GHGTHTAS +AG +V  AS L YA G A GMA
Sbjct: 184 RFFCGGYEATNGK-MNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 243

Query: 249 SRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSIAIGAFG 308
            +AR+AAYK+CW  GCYDSDILAA D A+ADGV +ISLSVG  G    YY D+IAIGAFG
Sbjct: 244 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVG--GVVVPYYLDAIAIGAFG 303

Query: 309 AMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVSLYS 368
           A+  G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++  GVS+Y 
Sbjct: 304 AIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYG 363

Query: 369 GDPLGDSKL-PLVYGGDC------GSRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAV 428
           G  L   ++ PLVYGG         S  C  GSL+ + V GKIV+CDRG N+R  KG  V
Sbjct: 364 GPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIV 423

Query: 429 KLAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYV------HSDPNPTATL 488
           +  GG GMI+AN   +GE L+AD H++P T VG   G+++R Y+       S  +PTAT+
Sbjct: 424 RKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATI 483

Query: 489 VFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDP 548
           VF+GT +G   PAP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+G++ D 
Sbjct: 484 VFKGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 543

Query: 549 RRVEFNIISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLAT 608
           RR EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SALITT+Y+VD SG P+ D +T
Sbjct: 544 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDEST 603

Query: 609 SEDSNPFVHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVK---DSSYS 668
              S+   +G+GH+ P +A++PGL+YD+ S DY++FLC+  Y    I    +   D   +
Sbjct: 604 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 663

Query: 669 QLCSHKFTNPGNLNYPSFAVVFDSDAEEVVK--YTRTVTNVGSEADAVYEVKVESPQGVL 728
           +   H     GNLNYPSF+VVF    E  +   + RTVTNVG ++D+VYE+K+  P+G  
Sbjct: 664 RRAGH----VGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVG-DSDSVYEIKIRPPRGTT 723

Query: 729 ISVLPNKLEFNAKKTTQSYEI----TFTKINGFKKSASFGSIEWSDGSHVVRSPIAVS 763
           ++V P KL F       S+ +    T  K++    +   G I WSDG   V SP+ V+
Sbjct: 724 VTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of MS009601 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 707.2 bits (1824), Expect = 1.4e-203
Identity = 377/769 (49.02%), Postives = 496/769 (64.50%), Query Frame = 0

Query: 6   VCSFLIFFFLLCFVPVIFSRLPDDQQTFIVHVSKSEKPALFSSHHHWHSTILESLSPSLH 65
           + S  I   L    P I        +TFI  +     P++F +H+HW+ST     S    
Sbjct: 1   MASSTIVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES---- 60

Query: 66  PTKLLYNYERAVNGFSARLTAAQAGELRRVPGVVSVIPDRIRQLHTTRTPHFLGLADNLG 125
             ++++ Y    +GFSA +T  +A  LR  P V++V  DR R+LHTTR+P FLGL +  G
Sbjct: 61  --RIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG 120

Query: 126 LWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPSSWKGICQTGEGFPASACNRKIIG 185
           LW +++Y  DVIIGV DTGIWPER SFSD  L P+P  W+G+C++G  F    CNRKIIG
Sbjct: 121 LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIG 180

Query: 186 ARAYFYGYASHLGKSFEDSSDLRSPRDTEGHGTHTASTAAGSFVKNASFLEYARGEARGM 245
           AR +  G  + +      + +  SPRD +GHGTHT+STAAG     AS   YA G A+G+
Sbjct: 181 ARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGV 240

Query: 246 ASRARIAAYKICW-ELGCYDSDILAAMDQAIADGVHIISLSV-GSSGRAPAYYRDSIAIG 305
           A +ARIAAYK+CW + GC DSDILAA D A+ DGV +IS+S+ G  G    YY D IAIG
Sbjct: 241 APKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIG 300

Query: 306 AFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFGGVS 365
           ++GA   G+ VS SAGN GP   +  N+APW+ TVGASTIDR F AD ILGDG    GVS
Sbjct: 301 SYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVS 360

Query: 366 LYSGDPLGDSKLPLVYGGDCG---SRYCYYGSLNSSKVAGKIVVCDRGGNARVEKGGAVK 425
           LY+G PL     P+VY G  G   +  C   +L+  +V GKIV+CDRG + RV KG  VK
Sbjct: 361 LYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVK 420

Query: 426 LAGGRGMILANTEENGEELLADAHLIPGTMVGEIAGNKLRDYVHSDPNPTATLVFRGTVI 485
            AGG GMILAN   NGE L+ DAHLIP   VG   G++++ Y  S PNP A++ FRGT++
Sbjct: 421 KAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIV 480

Query: 486 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWTGFSSPTGLSIDPRRVEFNI 545
           G   PAP +A+FS RGPN  + EILKPD+IAPGVNILA WT    PTGL  DPR+ EFNI
Sbjct: 481 G-IKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNI 540

Query: 546 ISGTSMSCPHVSGVAALLRKAFPAWSPAAIKSALITTSYSVDTSGSPIKDLATSEDSNPF 605
           +SGTSM+CPHVSG AALL+ A P WSPA I+SA++TT+  VD S   + D +T + + P+
Sbjct: 541 LSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPY 600

Query: 606 VHGAGHIDPNRALNPGLIYDLNSQDYVSFLCSIGYDPRQIAVFVKDSSYSQLCSHKFTNP 665
            +G+GH++  RA+NPGL+YD+ + DY++FLCSIGY P+ I V  +  +  +  + +  +P
Sbjct: 601 DYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITR--TPVRCPTTRKPSP 660

Query: 666 GNLNYPSFAVVFDSDAEEVVKYT--RTVTNVGSEADAVYEVKVESPQGVLISVLPNKLEF 725
           GNLNYPS   VF ++   +V  T  RT TNVG +A+AVY  ++ESP+GV ++V P +L F
Sbjct: 661 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVG-QAEAVYRARIESPRGVTVTVKPPRLVF 720

Query: 726 NAKKTTQSYEITFT----KINGFKKSASFGSIEWSD-GSHVVRSPIAVS 763
            +    +SY +T T     +   +  A FGS+ W D G HVVRSPI V+
Sbjct: 721 TSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVVT 759

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038897980.10.0e+0088.46subtilisin-like protease SBT1.4 [Benincasa hispida][more]
XP_022992130.10.0e+0086.90subtilisin-like protease SBT1.4 [Cucurbita maxima][more]
KAG6575439.10.0e+0086.80Subtilisin-like protease 1.4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022964269.10.0e+0086.90subtilisin-like protease SBT1.4 [Cucurbita moschata][more]
XP_022953749.10.0e+0086.66subtilisin-like protease SBT1.4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LVJ13.4e-29065.15Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... [more]
O653513.1e-22755.72Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9ZUF62.6e-21853.78Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Q9LUM38.4e-20950.00Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
O496072.0e-20249.02Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1JUV10.0e+0086.90subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3... [more]
A0A6J1HMN70.0e+0086.90subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE... [more]
A0A6J1GQK50.0e+0086.66subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE... [more]
A0A6J1KIR10.0e+0086.25subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111494328 PE=3... [more]
A0A0A0K6Z10.0e+0086.29Subtilase family protein OS=Cucumis sativus OX=3659 GN=Csa_7G448910 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14067.12.4e-29165.15Subtilase family protein [more]
AT5G67360.12.2e-22855.72Subtilase family protein [more]
AT2G05920.11.9e-21953.78Subtilase family protein [more]
AT3G14240.16.0e-21050.00Subtilase family protein [more]
AT4G34980.11.4e-20349.02subtilisin-like serine protease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 542..558
score: 59.45
coord: 212..225
score: 49.57
coord: 133..152
score: 31.98
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 32..110
e-value: 5.5E-17
score: 62.2
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 135..626
e-value: 1.5E-180
score: 602.9
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 109..617
NoneNo IPR availableGENE3D2.60.40.2310coord: 630..764
e-value: 8.1E-40
score: 137.6
NoneNo IPR availableGENE3D3.50.30.30coord: 344..481
e-value: 1.5E-180
score: 602.9
NoneNo IPR availablePANTHERPTHR10795:SF680SUBTILISIN-LIKE PROTEASE SBT1.6coord: 20..765
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 108..613
score: 27.07642
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 350..473
e-value: 6.3596E-37
score: 132.923
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 364..465
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 24..110
e-value: 1.0E-21
score: 79.2
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 134..582
e-value: 8.0E-51
score: 173.4
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 662..761
e-value: 4.6E-31
score: 106.9
IPR003137PA domainPFAMPF02225PAcoord: 375..459
e-value: 2.2E-10
score: 40.5
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 20..765
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 543..553
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 108..579
e-value: 9.15665E-145
score: 425.088

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009601.1MS009601.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity