MS009564 (gene) Bitter gourd (TR) v1

Overview
NameMS009564
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionGlucose-methanol-choline (Gmc) oxidoreductase
Locationscaffold813: 2320999 .. 2324849 (+)
RNA-Seq ExpressionMS009564
SyntenyMS009564
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATACCCGTTCATCAAGCGCGCAAGCTCGTTCTCGCGCAGCGGCCATGAGAGAGATAGAGCGAGAGAGAAAGGCTTCGATTACATAATCGTGGGCGGCGGCACCGCCGGATGTCCATTGGCGGCGACGCTGTCGCAGAAGTTCAGGGTTCTGGTGCTGGAAAGAGGCGGCGTTCCTTTCACGAATGCGAACGTGTCTTTGCTTCGGAACTTTCACATCGGCCTCGCCGATACTTCTCCGAGCTCGGCTTCTCAGGCCTTCGTCTCGACGGACGGAGTTATCAATGCGAGGGCCAGGGTCTTGGGTGGTGGTTCCGCCATTAATGCTGGCTTCTATACCAGAGCAAGTTCCAGGTAGTTCTATTTATTTCCCACCCGTTATAATTCTCTGATTTGATAAAAAAAAAAAAGAAAAAAAAAATTCTCTTTTTTTATTTTCTTTTGCAAAGGAAAAATTTTCTCTCTCACTTTTGTATTTTGTTCCCGATGTCTTAATTATATAATTTTATTAGAAATAATTGTGGAGACTAATAAGATGTGGTTTGATATCCATTTGGTTTTACTTTTTTGCTTTTAAAAATTTTGCTTGTTTTTATATAGAATTTTTAAAAATATATTGAAATTTTTAGCCAAATTTTAAATAAAAAACAAATTTTTAAGAATTATATTTTTTATTTTTTAAATTTAATGTTATATAAATTATTTAACGAATTGGTGAAAGTAATATTTTTAAGCTTAATTTTTAAAATGAAATAATTAAAAACGAAATAGTTATCTGACTAGGTCTAAATTATTTGAAATAATTTATCTTTTTTTTTTCTTAATTTATACTTTTTTTAGTACTATTTTTAACCTATAATTAGTATTAATATAATTGTTTTTTTTTCTTATTATAAGATTTTTAATCCAATAAGGTGAGGATTGAAATCTTCGACCTGTCATAATTAGTTAAACTATGTTCGAGTGAATGATTATGAGACTATCGNTCTTTTTTTTTTCTTAATCTATACTTTTTTTAGTACTATTTTTAACCTATAATTAGTATTAATATAATTGTTTTTTTTTCTTATTATAAGATTTTTAATCCAATAAGGTGAGGATTGAAATCTTCGACCTGTCATAATTAGTTAAACTATGTTCGAGTGAATGATTATGAGACTATCGTCAAACTACGAAAACCCTAAAAATAATAGTGTAAATTTATCCACTACTAATGATAATTAATTAGTAAAAGATTAGTTGGTAGCAATAATTCATCACTGGGCCTATATTAGAAAAGACAGTTCATGGCATAGAGTCTATTCAAATAATTCACTGCTAAATATGAAATTTTGAAAATCGCCTAGAAAACGACTTTTATACAATACTTTTTTAGTTGTTTTTATTCTCAAATTTATTTGTAATTATTATATTAAGTAGTGGTAAAAATAAATGTTGCTCTGCATTGGATTGTTGTTAACTTAACATATGATTTTGAGATAAAAGACAAAATATAGAAAACCAAGGATTAAATTTTAGAAAATAATTGTGTGAACATATTTGTTAATTTCATGACATCATTTATAATTTTTTCATTTCCCATTTGAATTATTTTGATTTTTTATTAAAAAACAATAATTGTGAGGTAGGTATAATGTGACCTATTTAAAGGGAAATTTAAGAACAAAATTCACTTATCTCAATTTCCACCCGCCTGTTAGCTCGAGCGAGTATAGTTCAAAATTGAAAATTTAAATGTAAATTTATTTTGTTAATAAAAACAGAAAATGGTAGTTTGTTTTTTTTTCTTTTAATTTTAGTATATAATATACATATAATTGATTGAGTGGAGACATATACATGTATATAAATATATCTACGTGACCACGTAAAATAATTATTTTCGTCTTTTTAAAATTTTATCCTTTGATAAATGAAAAATGAAAAATGAAAAATTAATCTATTTTATTTTCAAATTTGTTAATTGATGTATTATTAAATTATTATTTTATTAAGTCCAACTTCAAGTACATGTCAGTTACGGTTGATCAATATTCACGTTGTCATTTAAACATGTAGATATACAAATTAAAAGAAATTAATTTTATTGATAGACCAGTTAAATTTCTTGAATTGTCAAATATAGTTTCAATATAATGATCATTTTTGTAACAATTTTTTACTTTGATTCTGTTTAATGAACAACCTAATTAGAAATTAAAATTAAAGACGAAATTGTAACTAACAGTGCAGATTTATTTTCTCAAAAAAAAAAATTGCAGATTTATAAAGAAAGTGGGGTGGGACGCGAAGCTGGTGAACGAATCATACTCATGGGTGGAGAGGCAAATTGTTCACCGGCCGGAGCTCGCCGGCTGGCAGAAGGCTTTCACCGACAGTCTCTTAGACGTCGGAATCTCGCCCTTCAATGGCTTCACCTACGATCATCTCTACGGCACCAAAGTCGGAGGCACTATTTTCGACAGGTTCGGTCGCCGTCACACCACCGCCGAGCTCTTAGCTTCAGGGAATCCCGATAACCTTTCCGTTCTCGTTCACGCCACGGTTCAGAGACTCATTTTTCACTCCGCTCATGGTTCGTTTCCTTTGCTTCTTCTCTTTTGTTTTTGTTTTTCGCCATTCCGATTCGTTCCCTGAATTTAAAATGTGGGGTTCTTGGGTTAGGGTTTTCTCTGTTCGTCTCTTAGCCTAATCGGTTATCGATTTGCCCACTGCAGGTAAGAAACCGAAGGCGATTGGAGTTGTTTTCAAGGATGAAACTGGCAATCAGAACGAAGTCTTTCTCTCGAGTAAGCGGCAGAGTGAAGTTATTTTATCCAGTGGAGCAATTGCGACTCCGCAAATGCTGCTGCTCAGTGGAATTGGGCCGAGGGCTGATCTTGAGAAGTGGAACATCTCTGTGGTGCTTGACAATGAGTTTGTTGGGAAAGGCATGGCTGATAACCCCTTGAACTCCATTTTTGTTCCTAGCAACAGACCAGTTGAGCAGTCCCTTATTCAAGCAGTTGGAATCACCAAGCTTGGTGTTTATATTGAATCTAGCAGTGGATTTGGGCAGTCCGAGGAAAGTATTCACTGTCACCATGGTTTGATGTCAGCTGAGGTAAATTTAACACCTCCCCTTTTTTTTTACCAATCTTAAATCTGATTTTATGATGTTGGTTCTTTAGATAAGAACTTATGAGTTAAACTGTCTCCGCAAATGAGTGAATTAGATTGCAAGTATGTATGTAGAAAATGTTGATTCCGTGCGATGCTTAATTTTGTGGGTTTAACCAGATTGGGCAGCTATCCACCATTCCTCCAAAGCAAAGAACGCCAGAAGCCATTCAAGCTTACATAAAAAACAAACAAGACTTACCCCGTGAGGCATTTAAAGGAGGTTTTGTTCTAGAAAAAATAGCAAATCCCATCTCCAAAGGACAATTAAGCTTGATCAACACTAATGTTGACGACAATCCTGCCGTGACCTTCAACTACTTTGGTCATCCTAATGATCTTCACCGTTGCGTTGAAGGGGTTCGTATGGTTACAAAGATTGTACATTCTAAATACTTCACAAACTACACACAGTGCAATGAGGAAACATTGGACAAGCTGCTTAATATTAGTGTCAAAGCCAACATCAACCTCATACCTAAGCATACCAATGATACCAAGTCCTTGGAGCAGTTCTGCAGAGACACTGTGATCACAATCTGGCACTACCATGGAGGGTGCCTAGTGGGCAAGGTCGTGAACCATGACCGGAAGGTTCTCGGTGTAACTAGGCTGCGCATTGTCGATGGTTCAACATTCAGCGAGTCTCCTGGCACCAATCCTCAAGCTACTGTTATGATGATGGGAAGGTAT

mRNA sequence

CGATACCCGTTCATCAAGCGCGCAAGCTCGTTCTCGCGCAGCGGCCATGAGAGAGATAGAGCGAGAGAGAAAGGCTTCGATTACATAATCGTGGGCGGCGGCACCGCCGGATGTCCATTGGCGGCGACGCTGTCGCAGAAGTTCAGGGTTCTGGTGCTGGAAAGAGGCGGCGTTCCTTTCACGAATGCGAACGTGTCTTTGCTTCGGAACTTTCACATCGGCCTCGCCGATACTTCTCCGAGCTCGGCTTCTCAGGCCTTCGTCTCGACGGACGGAGTTATCAATGCGAGGGCCAGGGTCTTGGGTGGTGGTTCCGCCATTAATGCTGGCTTCTATACCAGAGCAAGTTCCAGATTTATAAAGAAAGTGGGGTGGGACGCGAAGCTGGTGAACGAATCATACTCATGGGTGGAGAGGCAAATTGTTCACCGGCCGGAGCTCGCCGGCTGGCAGAAGGCTTTCACCGACAGTCTCTTAGACGTCGGAATCTCGCCCTTCAATGGCTTCACCTACGATCATCTCTACGGCACCAAAGTCGGAGGCACTATTTTCGACAGGTTCGGTCGCCGTCACACCACCGCCGAGCTCTTAGCTTCAGGGAATCCCGATAACCTTTCCGTTCTCGTTCACGCCACGGTTCAGAGACTCATTTTTCACTCCGCTCATGGTAAGAAACCGAAGGCGATTGGAGTTGTTTTCAAGGATGAAACTGGCAATCAGAACGAAGTCTTTCTCTCGAGTAAGCGGCAGAGTGAAGTTATTTTATCCAGTGGAGCAATTGCGACTCCGCAAATGCTGCTGCTCAGTGGAATTGGGCCGAGGGCTGATCTTGAGAAGTGGAACATCTCTGTGGTGCTTGACAATGAGTTTGTTGGGAAAGGCATGGCTGATAACCCCTTGAACTCCATTTTTGTTCCTAGCAACAGACCAGTTGAGCAGTCCCTTATTCAAGCAGTTGGAATCACCAAGCTTGGTGTTTATATTGAATCTAGCAGTGGATTTGGGCAGTCCGAGGAAAGTATTCACTGTCACCATGGTTTGATGTCAGCTGAGATTGGGCAGCTATCCACCATTCCTCCAAAGCAAAGAACGCCAGAAGCCATTCAAGCTTACATAAAAAACAAACAAGACTTACCCCGTGAGGCATTTAAAGGAGGTTTTGTTCTAGAAAAAATAGCAAATCCCATCTCCAAAGGACAATTAAGCTTGATCAACACTAATGTTGACGACAATCCTGCCGTGACCTTCAACTACTTTGGTCATCCTAATGATCTTCACCGTTGCGTTGAAGGGGTTCGTATGGTTACAAAGATTGTACATTCTAAATACTTCACAAACTACACACAGTGCAATGAGGAAACATTGGACAAGCTGCTTAATATTAGTGTCAAAGCCAACATCAACCTCATACCTAAGCATACCAATGATACCAAGTCCTTGGAGCAGTTCTGCAGAGACACTGTGATCACAATCTGGCACTACCATGGAGGGTGCCTAGTGGGCAAGGTCGTGAACCATGACCGGAAGGTTCTCGGTGTAACTAGGCTGCGCATTGTCGATGGTTCAACATTCAGCGAGTCTCCTGGCACCAATCCTCAAGCTACTGTTATGATGATGGGAAGGTAT

Coding sequence (CDS)

CGATACCCGTTCATCAAGCGCGCAAGCTCGTTCTCGCGCAGCGGCCATGAGAGAGATAGAGCGAGAGAGAAAGGCTTCGATTACATAATCGTGGGCGGCGGCACCGCCGGATGTCCATTGGCGGCGACGCTGTCGCAGAAGTTCAGGGTTCTGGTGCTGGAAAGAGGCGGCGTTCCTTTCACGAATGCGAACGTGTCTTTGCTTCGGAACTTTCACATCGGCCTCGCCGATACTTCTCCGAGCTCGGCTTCTCAGGCCTTCGTCTCGACGGACGGAGTTATCAATGCGAGGGCCAGGGTCTTGGGTGGTGGTTCCGCCATTAATGCTGGCTTCTATACCAGAGCAAGTTCCAGATTTATAAAGAAAGTGGGGTGGGACGCGAAGCTGGTGAACGAATCATACTCATGGGTGGAGAGGCAAATTGTTCACCGGCCGGAGCTCGCCGGCTGGCAGAAGGCTTTCACCGACAGTCTCTTAGACGTCGGAATCTCGCCCTTCAATGGCTTCACCTACGATCATCTCTACGGCACCAAAGTCGGAGGCACTATTTTCGACAGGTTCGGTCGCCGTCACACCACCGCCGAGCTCTTAGCTTCAGGGAATCCCGATAACCTTTCCGTTCTCGTTCACGCCACGGTTCAGAGACTCATTTTTCACTCCGCTCATGGTAAGAAACCGAAGGCGATTGGAGTTGTTTTCAAGGATGAAACTGGCAATCAGAACGAAGTCTTTCTCTCGAGTAAGCGGCAGAGTGAAGTTATTTTATCCAGTGGAGCAATTGCGACTCCGCAAATGCTGCTGCTCAGTGGAATTGGGCCGAGGGCTGATCTTGAGAAGTGGAACATCTCTGTGGTGCTTGACAATGAGTTTGTTGGGAAAGGCATGGCTGATAACCCCTTGAACTCCATTTTTGTTCCTAGCAACAGACCAGTTGAGCAGTCCCTTATTCAAGCAGTTGGAATCACCAAGCTTGGTGTTTATATTGAATCTAGCAGTGGATTTGGGCAGTCCGAGGAAAGTATTCACTGTCACCATGGTTTGATGTCAGCTGAGATTGGGCAGCTATCCACCATTCCTCCAAAGCAAAGAACGCCAGAAGCCATTCAAGCTTACATAAAAAACAAACAAGACTTACCCCGTGAGGCATTTAAAGGAGGTTTTGTTCTAGAAAAAATAGCAAATCCCATCTCCAAAGGACAATTAAGCTTGATCAACACTAATGTTGACGACAATCCTGCCGTGACCTTCAACTACTTTGGTCATCCTAATGATCTTCACCGTTGCGTTGAAGGGGTTCGTATGGTTACAAAGATTGTACATTCTAAATACTTCACAAACTACACACAGTGCAATGAGGAAACATTGGACAAGCTGCTTAATATTAGTGTCAAAGCCAACATCAACCTCATACCTAAGCATACCAATGATACCAAGTCCTTGGAGCAGTTCTGCAGAGACACTGTGATCACAATCTGGCACTACCATGGAGGGTGCCTAGTGGGCAAGGTCGTGAACCATGACCGGAAGGTTCTCGGTGTAACTAGGCTGCGCATTGTCGATGGTTCAACATTCAGCGAGTCTCCTGGCACCAATCCTCAAGCTACTGTTATGATGATGGGAAGGTAT

Protein sequence

RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPFTNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFIKKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQNEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPLNSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFGHPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSLEQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMGRY
Homology
BLAST of MS009564 vs. NCBI nr
Match: XP_022154219.1 (protein HOTHEAD-like [Momordica charantia])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 541/542 (99.82%), Postives = 541/542 (99.82%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF
Sbjct: 31  RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 90

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI
Sbjct: 91  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 150

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 151 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 210

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHG KPKAIGVVFKDETGNQ
Sbjct: 211 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGMKPKAIGVVFKDETGNQ 270

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL
Sbjct: 271 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 330

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP
Sbjct: 331 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 390

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG
Sbjct: 391 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 450

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 451 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 510

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG
Sbjct: 511 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 570

Query: 541 RY 543
           RY
Sbjct: 571 RY 572

BLAST of MS009564 vs. NCBI nr
Match: XP_022953252.1 (protein HOTHEAD-like [Cucurbita moschata])

HSP 1 Score: 990.3 bits (2559), Expect = 6.4e-285
Identity = 484/542 (89.30%), Postives = 512/542 (94.46%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           +YPFIKRASSF R GHE    REKG+DYIIVGGGTAGCPLAATLSQ F VL+LERGGVPF
Sbjct: 35  QYPFIKRASSFYRDGHE----REKGYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPF 94

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVSLLRNFHIGLADTSP+SASQAF STDGVINARARVLGGGSAINAGFYTRASSRFI
Sbjct: 95  TNANVSLLRNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASSRFI 154

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWD KLVNESYSWVE++IVHRP+L  WQK FTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 155 KKVGWDEKLVNESYSWVEKRIVHRPKLTDWQKTFTDSLLDVGISPFNGFTYDHLYGTKVG 214

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELL SGNPDNL+VLVHATVQRLIF +AHGKKPKAIGVVFKDETGNQ
Sbjct: 215 GTIFDRFGRRHTTAELLPSGNPDNLTVLVHATVQRLIFDTAHGKKPKAIGVVFKDETGNQ 274

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           ++VFLSS+RQSEVILSSGAIATPQMLLLSGIGPR DLEKWNIS+VL NEFVGK MADNPL
Sbjct: 275 HKVFLSSRRQSEVILSSGAIATPQMLLLSGIGPRDDLEKWNISMVLHNEFVGKDMADNPL 334

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPV+QSLIQAVGITK GVYIESSSGFGQS ESIHCHHGL+SAEIGQLSTIPP
Sbjct: 335 NSIFVPSNRPVQQSLIQAVGITKRGVYIESSSGFGQSGESIHCHHGLLSAEIGQLSTIPP 394

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQ YIK+K+DLP EAFKGGF+LEK+ANP SKG LSLINTN+DDNPAVTFNYF 
Sbjct: 395 KQRTPEAIQNYIKSKRDLPHEAFKGGFILEKVANPFSKGHLSLINTNIDDNPAVTFNYFH 454

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HP DLHRCVEGVRM+TKI  S+YFTN+TQC++ETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 455 HPYDLHRCVEGVRMLTKIADSEYFTNFTQCDKETLDKLLNISVKANINLIPKHTNDTKSL 514

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFC+DTVITIWHYHGGCLV KVV+HD KVLGVTRLRI+DGSTFSESPGTNPQATVMMMG
Sbjct: 515 EQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLRIIDGSTFSESPGTNPQATVMMMG 572

Query: 541 RY 543
           RY
Sbjct: 575 RY 572

BLAST of MS009564 vs. NCBI nr
Match: XP_008462353.1 (PREDICTED: protein HOTHEAD-like [Cucumis melo])

HSP 1 Score: 989.2 bits (2556), Expect = 1.4e-284
Identity = 487/542 (89.85%), Postives = 511/542 (94.28%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           RYPFIKRASSF R  HE    R+ G+DYIIVGGGTAGCPLAATLSQ F VL+LERGGVPF
Sbjct: 35  RYPFIKRASSFYRDSHE----RKGGYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPF 94

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVS LRNFHIGLADTSP+SASQAF STDGVINARARVLGGGSAINAGFYTRAS+RFI
Sbjct: 95  TNANVSFLRNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASTRFI 154

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWD KLVNESYSWVE +IVHRP+LA WQKAFTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 155 KKVGWDEKLVNESYSWVENRIVHRPKLADWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 214

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELLASGNPD L+VLVHATVQRLIF +  GKKPKAIGVVFKD+ GNQ
Sbjct: 215 GTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLIFDTTDGKKPKAIGVVFKDDIGNQ 274

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           +EVFLSS RQSEVI+SSGAI TPQMLLLSGIGPRADLEKWNIS+VLDN+FVGK MADNPL
Sbjct: 275 HEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADLEKWNISMVLDNDFVGKDMADNPL 334

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPV+QSLIQAVGITK GVYIESSSGFGQS ESIHCHHGLMSAEIGQLSTIPP
Sbjct: 335 NSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQSGESIHCHHGLMSAEIGQLSTIPP 394

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQAYIK+K+DLP EAFKGGFVLEKIANPIS+GQLSLINTNVDDNPAVTFNYFG
Sbjct: 395 KQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPISRGQLSLINTNVDDNPAVTFNYFG 454

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HP DLHRCVEG+RMVTKIV SK FTN+TQC++ETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 455 HPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDKLLNISVKANINLIPKHTNDTKSL 514

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFC+DTVITIWHYHGGCLV KVV+HD KVLGVTRLRIVDGSTFSESPGTNPQATVMMMG
Sbjct: 515 EQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 572

Query: 541 RY 543
           RY
Sbjct: 575 RY 572

BLAST of MS009564 vs. NCBI nr
Match: XP_038899986.1 (protein HOTHEAD-like [Benincasa hispida])

HSP 1 Score: 988.8 bits (2555), Expect = 1.9e-284
Identity = 487/542 (89.85%), Postives = 508/542 (93.73%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           RYPFIKRASSF R  HE    RE G+DYIIVGGGTAGCPLAATLSQ F VL+LERGGVPF
Sbjct: 35  RYPFIKRASSFYRDSHE----RENGYDYIIVGGGTAGCPLAATLSQNFTVLLLERGGVPF 94

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVS L+NFHIGLADTSP+SASQAF STDGVINARARVLGGGSAINAGFYTRAS+RFI
Sbjct: 95  TNANVSFLQNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASTRFI 154

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
            KVGWD KLVNESYSWVE QIVHRP+LA WQKAFTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 155 NKVGWDEKLVNESYSWVENQIVHRPKLADWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 214

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHT AELLASGNPD L+VLVHATVQRLIF +  GKKPKAIGVVF D+TGNQ
Sbjct: 215 GTIFDRFGRRHTAAELLASGNPDKLTVLVHATVQRLIFDTTEGKKPKAIGVVFMDDTGNQ 274

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           +EVFLSS RQSEVILS+GAI TPQMLLLSGIGPRADLEKWNIS+VLDNEFVGK MADNPL
Sbjct: 275 HEVFLSSSRQSEVILSNGAIGTPQMLLLSGIGPRADLEKWNISMVLDNEFVGKDMADNPL 334

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPV+QSLIQAVGITKLGVYIESSSGFGQS ESIHCHHGLMSAEIGQLSTIPP
Sbjct: 335 NSIFVPSNRPVKQSLIQAVGITKLGVYIESSSGFGQSGESIHCHHGLMSAEIGQLSTIPP 394

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQ YIK+K+DLP EAFKGGFVLEKIANPIS+G LSLINTNVDDNPAVTFNYFG
Sbjct: 395 KQRTPEAIQDYIKSKRDLPHEAFKGGFVLEKIANPISRGHLSLINTNVDDNPAVTFNYFG 454

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HP DLHRCVEGVRM+TKIV SKYFTN+TQC+EETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 455 HPYDLHRCVEGVRMLTKIVESKYFTNFTQCDEETLDKLLNISVKANINLIPKHTNDTKSL 514

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFC+DTVITIWHYHGGCLV KVV+HD KVLGVTRLRIVDGST SESPGTNPQATVMMMG
Sbjct: 515 EQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLRIVDGSTLSESPGTNPQATVMMMG 572

Query: 541 RY 543
           RY
Sbjct: 575 RY 572

BLAST of MS009564 vs. NCBI nr
Match: KAA0059432.1 (protein HOTHEAD-like [Cucumis melo var. makuwa] >TYK03893.1 protein HOTHEAD-like [Cucumis melo var. makuwa])

HSP 1 Score: 988.0 bits (2553), Expect = 3.2e-284
Identity = 486/542 (89.67%), Postives = 511/542 (94.28%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           RYPFIKRASSF R  HE    ++ G+DYIIVGGGTAGCPLAATLSQ F VL+LERGGVPF
Sbjct: 35  RYPFIKRASSFYRDSHE----KKGGYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPF 94

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVS LRNFHIGLADTSP+SASQAF STDGVINARARVLGGGSAINAGFYTRAS+RFI
Sbjct: 95  TNANVSFLRNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASTRFI 154

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWD KLVNESYSWVE +IVHRP+LA WQKAFTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 155 KKVGWDEKLVNESYSWVENRIVHRPKLADWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 214

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELLASGNPD L+VLVHATVQRLIF +  GKKPKAIGVVFKD+ GNQ
Sbjct: 215 GTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLIFDTTDGKKPKAIGVVFKDDIGNQ 274

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           +EVFLSS RQSEVI+SSGAI TPQMLLLSGIGPRADLEKWNIS+VLDN+FVGK MADNPL
Sbjct: 275 HEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADLEKWNISMVLDNDFVGKDMADNPL 334

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPV+QSLIQAVGITK GVYIESSSGFGQS ESIHCHHGLMSAEIGQLSTIPP
Sbjct: 335 NSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQSGESIHCHHGLMSAEIGQLSTIPP 394

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQAYIK+K+DLP EAFKGGFVLEKIANPIS+GQLSLINTNVDDNPAVTFNYFG
Sbjct: 395 KQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPISRGQLSLINTNVDDNPAVTFNYFG 454

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HP DLHRCVEG+RMVTKIV SK FTN+TQC++ETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 455 HPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDKLLNISVKANINLIPKHTNDTKSL 514

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFC+DTVITIWHYHGGCLV KVV+HD KVLGVTRLRIVDGSTFSESPGTNPQATVMMMG
Sbjct: 515 EQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 572

Query: 541 RY 543
           RY
Sbjct: 575 RY 572

BLAST of MS009564 vs. ExPASy Swiss-Prot
Match: Q9S746 (Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 5.9e-225
Identity = 373/544 (68.57%), Postives = 448/544 (82.35%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHE--RDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGV 60
           RY FI +AS+FS S         ++  +DYI++GGGTAGCPLAATLSQ F VLVLERGGV
Sbjct: 36  RYTFIDKASTFSSSSSSSFSSNGQDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGV 95

Query: 61  PFTNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSR 120
           PFTNANVS LRNFHIGLAD S SSASQAFVSTDGV NARARVLGGGS INAGFY+RA + 
Sbjct: 96  PFTNANVSFLRNFHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAA 155

Query: 121 FIKKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTK 180
           F+K+ GWD KLV ESY WVER+IVH+P+L  WQKA  DSLL+VG+ PFNGFTYDH+ GTK
Sbjct: 156 FVKRAGWDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTK 215

Query: 181 VGGTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETG 240
           +GGTIFDRFGRRHT AELLA  NP  L VL++ATVQ+++F ++ G +P+  GV+FKDE G
Sbjct: 216 IGGTIFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTS-GTRPRVTGVIFKDEKG 275

Query: 241 NQNEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADN 300
           NQ++  LS+++ SEVILSSGAI +PQML+LSGIGP+ +L++  I VVL+NE VGKGMADN
Sbjct: 276 NQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADN 335

Query: 301 PLNSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTI 360
           P+N+I VPS  P+EQSLIQ VGITK+GVY+E+S+GFGQS ESIH H+G+MS +    STI
Sbjct: 336 PMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTI 395

Query: 361 PPKQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNY 420
           P KQR PEA QAYI   +    EAF G F+LEK+A PIS+G LSL+NTNVDDNP+VTFNY
Sbjct: 396 PAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNY 455

Query: 421 FGHPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTK 480
           F HP DL RCVE +R+V+K+V S  F NYTQC+++ + K+L++SVKANINL PK  NDTK
Sbjct: 456 FKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTK 515

Query: 481 SLEQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMM 540
           S+ QFC+DTV+TIWHYHGGCLVGKVV+ +RKVLGV RLR++DGSTF ESPGTNPQAT+MM
Sbjct: 516 SMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMM 575

Query: 541 MGRY 543
           MGRY
Sbjct: 576 MGRY 578

BLAST of MS009564 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.5e-124
Identity = 253/547 (46.25%), Postives = 330/547 (60.33%), Query Frame = 0

Query: 3   PFIKRASSFSR-SGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPFT 62
           P + R   F R   +  D A E  +DYIIVGGGTAGCPLAATLSQ FRVL+LERGGVP+ 
Sbjct: 30  PRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYN 89

Query: 63  NANVSLLRNFHIGLADTSP-SSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 122
             NV     F   L D +   S +Q+F+S +GV NAR RVLGG SAINAGFY+RA  +F 
Sbjct: 90  RPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFF 149

Query: 123 KKVG--WDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTK 182
           +  G  WD   VN+SY WVER IV RP+L  WQ A  D+LL+VG+ PFNGFT +H  GTK
Sbjct: 150 ENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTK 209

Query: 183 VGGTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSA---HGKKPKAIGVVFKD 242
           +GG+ FDR GRRH++A+LL      N+ V V+ATV+R++  S+    G    AIGVV++D
Sbjct: 210 IGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRD 269

Query: 243 ETGNQNEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGM 302
           + G  +   +  + + EVILS+GA+ +PQ+L LSGIGPR+ L  W I V LD   VG  +
Sbjct: 270 QLGRFHHALI--RDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFV 329

Query: 303 ADNPLNSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQL 362
            DNP N I +    P+E SLIQ VG+T+ G ++E++S        +H      S  I   
Sbjct: 330 YDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLH------SVFI--- 389

Query: 363 STIPPKQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVT 422
                  R P A   Y+               ++EKI  P+S G L L +T+V  NP V 
Sbjct: 390 -------RAP-ASPLYVPVTT-----------IMEKILGPVSIGLLRLASTDVRINPVVR 449

Query: 423 FNYFGHPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTN 482
           FNYF  P DL RCV G R + +I+ S+   ++        +   N   +     +P   +
Sbjct: 450 FNYFSDPQDLERCVNGTRKIGEILRSRAMQDFM-----IREWFGNRRFRFVGAPLPVDQS 509

Query: 483 DTKSLEQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQAT 542
           +   +  FCR TV TIWHYHGG +VGKVV+ D KV+GV  LR+VDGSTF+ SPGTNPQAT
Sbjct: 510 NDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQAT 541

BLAST of MS009564 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 4.1e-101
Identity = 207/528 (39.20%), Postives = 294/528 (55.68%), Query Frame = 0

Query: 19  DRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPFTNANVSLLRNFHIGL-AD 78
           D   E  +DY+IVGGGT+GCPLAATLS+K++VLVLERG +P    NV     F   L  +
Sbjct: 47  DLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQE 106

Query: 79  TSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFIKKVG--WDAKLVNESYS 138
               +  + FVS DG+ N R RVLGG S INAG Y RA++      G  WD  LVN++Y 
Sbjct: 107 DDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYE 166

Query: 139 WVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAE 198
           WVE  IV +P    WQ     + L+ G+ P +GF+ DH  GT++ G+ FD  G RH   E
Sbjct: 167 WVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADE 226

Query: 199 LLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQNEVFLSSKRQSEVIL 258
           LL  GN +NL V VHA+V+++IF +A G    A GV+++D  G  +  F+ SK   EVI+
Sbjct: 227 LLNKGNSNNLRVGVHASVEKIIFSNAPGL--TATGVIYRDSNGTPHRAFVRSK--GEVIV 286

Query: 259 SSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPLNSIFVPSNRPVEQSL 318
           S+G I TPQ+LLLSG+GP + L   NI VVL + +VG+ + DNP N I +    P+E ++
Sbjct: 287 SAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTI 346

Query: 319 IQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKNK 378
           +  +GI               S +   C    +        T PP    P          
Sbjct: 347 VTVLGI---------------SNDFYQCSFSSLP------FTTPPFSFFP-------STS 406

Query: 379 QDLPREAFKGGFVLEKIANPISKGQLSL-INTNVDDNPAVTFNYFGHPNDLHRCVEGVRM 438
             LP   F       K+A P+S G L+L  ++NV  +P V FNY+ +P DL  CV G++ 
Sbjct: 407 YPLPNSTF--AHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKK 466

Query: 439 VTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSLEQFCRDTVITIWHY 498
           + +++ +     Y   +   ++    + +      +PK   D  + E FCR++V + WHY
Sbjct: 467 IGELLSTDALKPYKVEDLPGIEGFNILGIP-----LPKDQTDDAAFETFCRESVASYWHY 526

Query: 499 HGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMGRY 543
           HGGCLVGKV++ D +V G+  LR+VDGSTF  +P ++PQ   +M+GRY
Sbjct: 527 HGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRY 535

BLAST of MS009564 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 5.4e-101
Identity = 209/532 (39.29%), Postives = 297/532 (55.83%), Query Frame = 0

Query: 19  DRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPFTNANVSLLRNFHIGL-AD 78
           D   E  +DY+IVGGGT+GCPLAATLS+K++VLVLERG +P    NV     F   L  +
Sbjct: 47  DLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQE 106

Query: 79  TSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFIKKVG--WDAKLVNESYS 138
               +  + FVS DG+ N R RVLGG S INAG Y RA++      G  WD  LVN++Y 
Sbjct: 107 DDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYE 166

Query: 139 WVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAE 198
           WVE  IV++P    WQ     + L+ G+ P +GF+ DH  GT++ G+ FD  G RH   E
Sbjct: 167 WVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADE 226

Query: 199 LLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQNEVFLSSKRQSEVIL 258
           LL  GN +NL V VHA+V+++IF +A G    A GV+++D  G  ++ F+ SK   EVI+
Sbjct: 227 LLNKGNSNNLRVGVHASVEKIIFSNAPGL--TATGVIYRDSNGTPHQAFVRSK--GEVIV 286

Query: 259 SSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPLNSIFVPSNRPVEQSL 318
           S+G I TPQ+LLLSG+GP + L   NI VVL + +VG+ + DNP N I +    P+E ++
Sbjct: 287 SAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTI 346

Query: 319 IQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKNK 378
           +  +GI               S +   C    +        T PP    P A        
Sbjct: 347 VTVLGI---------------SNDFYQCSFSSLP------FTTPPFGFFPSA-------S 406

Query: 379 QDLPREAFKGGFVLEKIANPISKGQLSL-INTNVDDNPAVTFNYFGHPNDLHRCVEGVRM 438
             LP   F       K+A P+S G L+L  ++NV  +P V FNY+ +  DL  CV G++ 
Sbjct: 407 YPLPNSTF--AHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKK 466

Query: 439 VTKIVHSKYFTNYTQCNEETLDKLLNISVKANINL----IPKHTNDTKSLEQFCRDTVIT 498
           + +++ +     Y         K+ ++      N+    +PK   D  + E FCR++V +
Sbjct: 467 IGELLSTDALKPY---------KVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVAS 526

Query: 499 IWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMGRY 543
            WHYHGGCLVGKV++ D +V G+  LR+VDGSTF  +P ++PQ   +M+GRY
Sbjct: 527 YWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRY 535

BLAST of MS009564 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 5.0e-99
Identity = 205/528 (38.83%), Postives = 296/528 (56.06%), Query Frame = 0

Query: 19  DRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPFTNANVSLLRNFHIGL-AD 78
           D   E  +DYIIVGGGTAGCPLAATLS  + VLVLERG +P    N+ +   F   L  +
Sbjct: 47  DPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQE 106

Query: 79  TSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFIKKVG--WDAKLVNESYS 138
               +  + FVS DG+ N R RVLGG S INAG Y RA++ F  + G  WD  LVN++Y 
Sbjct: 107 DDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYE 166

Query: 139 WVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAE 198
           WVE  IV  P+   WQ     + L+ GI P NGF+ DHL GT++ G+ FD  G RH + E
Sbjct: 167 WVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDE 226

Query: 199 LLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQNEVFLSSKRQSEVIL 258
           LL  G+P+NL V V A V+++IF S++     AIGV++ D  G  ++ F+  + + EVIL
Sbjct: 227 LLNKGDPNNLRVAVQAAVEKIIF-SSNTSGVTAIGVIYTDSNGTTHQAFV--RGEGEVIL 286

Query: 259 SSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPLNSIFVPSNRPVEQSL 318
           S+G I +PQ+LLLSG+GP + L   NISVV  + +VG+ + DNP N I +    P+E S 
Sbjct: 287 SAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEAST 346

Query: 319 IQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKNK 378
           +  +GIT         S F Q      C    +  +    S  P                
Sbjct: 347 VTVLGIT---------SDFYQ------CSISSLPFDTPPFSFFP-------------TTS 406

Query: 379 QDLPREAFKGGFVLEKIANPISKGQLSL-INTNVDDNPAVTFNYFGHPNDLHRCVEGVRM 438
             LP + F    ++ K+  P+S G ++L  +++V   P V FNY+ +  DL  CV G++ 
Sbjct: 407 YPLPNQTF--AHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKK 466

Query: 439 VTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSLEQFCRDTVITIWHY 498
           + +++ +     Y   +   +D    + +      +P++  D  + E FCR++V + WHY
Sbjct: 467 LGEVLSTDALEPYKVEDLPGIDGFNILGIP-----LPENQTDDAAFETFCRESVASYWHY 526

Query: 499 HGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMGRY 543
           HGGCLVGKV++   +V G+  LR+VDGSTF  +P ++PQ   +M+GRY
Sbjct: 527 HGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRY 536

BLAST of MS009564 vs. ExPASy TrEMBL
Match: A0A6J1DLE3 (protein HOTHEAD-like OS=Momordica charantia OX=3673 GN=LOC111021526 PE=3 SV=1)

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 541/542 (99.82%), Postives = 541/542 (99.82%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF
Sbjct: 31  RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 90

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI
Sbjct: 91  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 150

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 151 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 210

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHG KPKAIGVVFKDETGNQ
Sbjct: 211 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGMKPKAIGVVFKDETGNQ 270

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL
Sbjct: 271 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 330

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP
Sbjct: 331 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 390

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG
Sbjct: 391 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 450

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 451 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 510

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG
Sbjct: 511 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 570

Query: 541 RY 543
           RY
Sbjct: 571 RY 572

BLAST of MS009564 vs. ExPASy TrEMBL
Match: A0A6J1GMR0 (protein HOTHEAD-like OS=Cucurbita moschata OX=3662 GN=LOC111455858 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.1e-285
Identity = 484/542 (89.30%), Postives = 512/542 (94.46%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           +YPFIKRASSF R GHE    REKG+DYIIVGGGTAGCPLAATLSQ F VL+LERGGVPF
Sbjct: 35  QYPFIKRASSFYRDGHE----REKGYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPF 94

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVSLLRNFHIGLADTSP+SASQAF STDGVINARARVLGGGSAINAGFYTRASSRFI
Sbjct: 95  TNANVSLLRNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASSRFI 154

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWD KLVNESYSWVE++IVHRP+L  WQK FTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 155 KKVGWDEKLVNESYSWVEKRIVHRPKLTDWQKTFTDSLLDVGISPFNGFTYDHLYGTKVG 214

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELL SGNPDNL+VLVHATVQRLIF +AHGKKPKAIGVVFKDETGNQ
Sbjct: 215 GTIFDRFGRRHTTAELLPSGNPDNLTVLVHATVQRLIFDTAHGKKPKAIGVVFKDETGNQ 274

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           ++VFLSS+RQSEVILSSGAIATPQMLLLSGIGPR DLEKWNIS+VL NEFVGK MADNPL
Sbjct: 275 HKVFLSSRRQSEVILSSGAIATPQMLLLSGIGPRDDLEKWNISMVLHNEFVGKDMADNPL 334

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPV+QSLIQAVGITK GVYIESSSGFGQS ESIHCHHGL+SAEIGQLSTIPP
Sbjct: 335 NSIFVPSNRPVQQSLIQAVGITKRGVYIESSSGFGQSGESIHCHHGLLSAEIGQLSTIPP 394

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQ YIK+K+DLP EAFKGGF+LEK+ANP SKG LSLINTN+DDNPAVTFNYF 
Sbjct: 395 KQRTPEAIQNYIKSKRDLPHEAFKGGFILEKVANPFSKGHLSLINTNIDDNPAVTFNYFH 454

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HP DLHRCVEGVRM+TKI  S+YFTN+TQC++ETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 455 HPYDLHRCVEGVRMLTKIADSEYFTNFTQCDKETLDKLLNISVKANINLIPKHTNDTKSL 514

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFC+DTVITIWHYHGGCLV KVV+HD KVLGVTRLRI+DGSTFSESPGTNPQATVMMMG
Sbjct: 515 EQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLRIIDGSTFSESPGTNPQATVMMMG 572

Query: 541 RY 543
           RY
Sbjct: 575 RY 572

BLAST of MS009564 vs. ExPASy TrEMBL
Match: A0A1S3CIA9 (protein HOTHEAD-like OS=Cucumis melo OX=3656 GN=LOC103500726 PE=3 SV=1)

HSP 1 Score: 989.2 bits (2556), Expect = 6.9e-285
Identity = 487/542 (89.85%), Postives = 511/542 (94.28%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           RYPFIKRASSF R  HE    R+ G+DYIIVGGGTAGCPLAATLSQ F VL+LERGGVPF
Sbjct: 35  RYPFIKRASSFYRDSHE----RKGGYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPF 94

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVS LRNFHIGLADTSP+SASQAF STDGVINARARVLGGGSAINAGFYTRAS+RFI
Sbjct: 95  TNANVSFLRNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASTRFI 154

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWD KLVNESYSWVE +IVHRP+LA WQKAFTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 155 KKVGWDEKLVNESYSWVENRIVHRPKLADWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 214

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELLASGNPD L+VLVHATVQRLIF +  GKKPKAIGVVFKD+ GNQ
Sbjct: 215 GTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLIFDTTDGKKPKAIGVVFKDDIGNQ 274

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           +EVFLSS RQSEVI+SSGAI TPQMLLLSGIGPRADLEKWNIS+VLDN+FVGK MADNPL
Sbjct: 275 HEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADLEKWNISMVLDNDFVGKDMADNPL 334

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPV+QSLIQAVGITK GVYIESSSGFGQS ESIHCHHGLMSAEIGQLSTIPP
Sbjct: 335 NSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQSGESIHCHHGLMSAEIGQLSTIPP 394

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQAYIK+K+DLP EAFKGGFVLEKIANPIS+GQLSLINTNVDDNPAVTFNYFG
Sbjct: 395 KQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPISRGQLSLINTNVDDNPAVTFNYFG 454

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HP DLHRCVEG+RMVTKIV SK FTN+TQC++ETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 455 HPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDKLLNISVKANINLIPKHTNDTKSL 514

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFC+DTVITIWHYHGGCLV KVV+HD KVLGVTRLRIVDGSTFSESPGTNPQATVMMMG
Sbjct: 515 EQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 572

Query: 541 RY 543
           RY
Sbjct: 575 RY 572

BLAST of MS009564 vs. ExPASy TrEMBL
Match: A0A5A7UU55 (Protein HOTHEAD-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00880 PE=3 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 1.5e-284
Identity = 486/542 (89.67%), Postives = 511/542 (94.28%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           RYPFIKRASSF R  HE    ++ G+DYIIVGGGTAGCPLAATLSQ F VL+LERGGVPF
Sbjct: 35  RYPFIKRASSFYRDSHE----KKGGYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPF 94

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVS LRNFHIGLADTSP+SASQAF STDGVINARARVLGGGSAINAGFYTRAS+RFI
Sbjct: 95  TNANVSFLRNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASTRFI 154

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWD KLVNESYSWVE +IVHRP+LA WQKAFTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 155 KKVGWDEKLVNESYSWVENRIVHRPKLADWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 214

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELLASGNPD L+VLVHATVQRLIF +  GKKPKAIGVVFKD+ GNQ
Sbjct: 215 GTIFDRFGRRHTTAELLASGNPDKLTVLVHATVQRLIFDTTDGKKPKAIGVVFKDDIGNQ 274

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           +EVFLSS RQSEVI+SSGAI TPQMLLLSGIGPRADLEKWNIS+VLDN+FVGK MADNPL
Sbjct: 275 HEVFLSSNRQSEVIMSSGAIGTPQMLLLSGIGPRADLEKWNISMVLDNDFVGKDMADNPL 334

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPV+QSLIQAVGITK GVYIESSSGFGQS ESIHCHHGLMSAEIGQLSTIPP
Sbjct: 335 NSIFVPSNRPVKQSLIQAVGITKRGVYIESSSGFGQSGESIHCHHGLMSAEIGQLSTIPP 394

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQAYIK+K+DLP EAFKGGFVLEKIANPIS+GQLSLINTNVDDNPAVTFNYFG
Sbjct: 395 KQRTPEAIQAYIKSKRDLPHEAFKGGFVLEKIANPISRGQLSLINTNVDDNPAVTFNYFG 454

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HP DLHRCVEG+RMVTKIV SK FTN+TQC++ETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 455 HPYDLHRCVEGIRMVTKIVESKCFTNFTQCDKETLDKLLNISVKANINLIPKHTNDTKSL 514

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFC+DTVITIWHYHGGCLV KVV+HD KVLGVTRLRIVDGSTFSESPGTNPQATVMMMG
Sbjct: 515 EQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 572

Query: 541 RY 543
           RY
Sbjct: 575 RY 572

BLAST of MS009564 vs. ExPASy TrEMBL
Match: A0A6J1JWY5 (protein HOTHEAD-like OS=Cucurbita maxima OX=3661 GN=LOC111488603 PE=3 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 5.8e-284
Identity = 481/542 (88.75%), Postives = 509/542 (93.91%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHERDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPF 60
           +YPFIKRASSF R GHE    REKG+DYIIVGGGTAGCPLAATLSQ F VL+LERGGVPF
Sbjct: 35  QYPFIKRASSFYRDGHE----REKGYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPF 94

Query: 61  TNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSRFI 120
           TNANVSLLRNFHIGLADTSP+SASQAF STDGVINARARVLGGGSAINAGFYTRASSRFI
Sbjct: 95  TNANVSLLRNFHIGLADTSPTSASQAFASTDGVINARARVLGGGSAINAGFYTRASSRFI 154

Query: 121 KKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVG 180
           KKVGWD KLVNESYSWVE++IVHRP+L  WQK FTDSLLDVGISPFNGFTYDHLYGTKVG
Sbjct: 155 KKVGWDEKLVNESYSWVEKRIVHRPKLTDWQKTFTDSLLDVGISPFNGFTYDHLYGTKVG 214

Query: 181 GTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQ 240
           GTIFDRFGRRHTTAELL SGNPDNL VLVHATVQRLIF + HGKKPKAIGVVFKDETGN 
Sbjct: 215 GTIFDRFGRRHTTAELLPSGNPDNLMVLVHATVQRLIFDTTHGKKPKAIGVVFKDETGNP 274

Query: 241 NEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPL 300
           ++VFLSS+RQSEVILSSGAIATPQMLLLSGIGPR DLEKWNIS+VL NEFVGK MADNPL
Sbjct: 275 HKVFLSSRRQSEVILSSGAIATPQMLLLSGIGPRDDLEKWNISMVLHNEFVGKDMADNPL 334

Query: 301 NSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPP 360
           NSIFVPSNRPV+QSLIQAVGITK GVYIESSSGFGQS ESIHCHHGL+SAEIGQLSTIPP
Sbjct: 335 NSIFVPSNRPVQQSLIQAVGITKHGVYIESSSGFGQSRESIHCHHGLLSAEIGQLSTIPP 394

Query: 361 KQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFG 420
           KQRTPEAIQ YIK+K+D+P EAFKGGF+LEK+ANP SKG LSLINTN+DDNPAVTFNYF 
Sbjct: 395 KQRTPEAIQTYIKSKRDIPHEAFKGGFILEKVANPFSKGHLSLINTNIDDNPAVTFNYFH 454

Query: 421 HPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL 480
           HP DLHRCVEGVRM+TKI  S+YFTN+TQC++ETLDKLLNISVKANINLIPKHTNDTKSL
Sbjct: 455 HPYDLHRCVEGVRMLTKIADSEYFTNFTQCDKETLDKLLNISVKANINLIPKHTNDTKSL 514

Query: 481 EQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMG 540
           EQFC+DTVITIWHYHGGCLV KVV+HD KVLGVTRLRI+DGSTFSESPGTNPQATVMMMG
Sbjct: 515 EQFCKDTVITIWHYHGGCLVDKVVSHDLKVLGVTRLRIIDGSTFSESPGTNPQATVMMMG 572

Query: 541 RY 543
           RY
Sbjct: 575 RY 572

BLAST of MS009564 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 781.6 bits (2017), Expect = 4.2e-226
Identity = 373/544 (68.57%), Postives = 448/544 (82.35%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHE--RDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGV 60
           RY FI +AS+FS S         ++  +DYI++GGGTAGCPLAATLSQ F VLVLERGGV
Sbjct: 36  RYTFIDKASTFSSSSSSSFSSNGQDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGV 95

Query: 61  PFTNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSR 120
           PFTNANVS LRNFHIGLAD S SSASQAFVSTDGV NARARVLGGGS INAGFY+RA + 
Sbjct: 96  PFTNANVSFLRNFHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAA 155

Query: 121 FIKKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTK 180
           F+K+ GWD KLV ESY WVER+IVH+P+L  WQKA  DSLL+VG+ PFNGFTYDH+ GTK
Sbjct: 156 FVKRAGWDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTK 215

Query: 181 VGGTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETG 240
           +GGTIFDRFGRRHT AELLA  NP  L VL++ATVQ+++F ++ G +P+  GV+FKDE G
Sbjct: 216 IGGTIFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTS-GTRPRVTGVIFKDEKG 275

Query: 241 NQNEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADN 300
           NQ++  LS+++ SEVILSSGAI +PQML+LSGIGP+ +L++  I VVL+NE VGKGMADN
Sbjct: 276 NQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADN 335

Query: 301 PLNSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTI 360
           P+N+I VPS  P+EQSLIQ VGITK+GVY+E+S+GFGQS ESIH H+G+MS +    STI
Sbjct: 336 PMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTI 395

Query: 361 PPKQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNY 420
           P KQR PEA QAYI   +    EAF G F+LEK+A PIS+G LSL+NTNVDDNP+VTFNY
Sbjct: 396 PAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNY 455

Query: 421 FGHPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTK 480
           F HP DL RCVE +R+V+K+V S  F NYTQC+++ + K+L++SVKANINL PK  NDTK
Sbjct: 456 FKHPVDLQRCVEAIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTK 515

Query: 481 SLEQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMM 540
           S+ QFC+DTV+TIWHYHGGCLVGKVV+ +RKVLGV RLR++DGSTF ESPGTNPQAT+MM
Sbjct: 516 SMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMM 575

Query: 541 MGRY 543
           MGRY
Sbjct: 576 MGRY 578

BLAST of MS009564 vs. TAIR 10
Match: AT1G72970.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 733.4 bits (1892), Expect = 1.3e-211
Identity = 357/544 (65.62%), Postives = 428/544 (78.68%), Query Frame = 0

Query: 1   RYPFIKRASSFSRSGHE--RDRAREKGFDYIIVGGGTAGCPLAATLSQKFRVLVLERGGV 60
           RY FI +AS+FS S         ++  +DYI++GGGTAGCPLAATLSQ F VLVLERGGV
Sbjct: 36  RYTFIDKASTFSSSSSSSFSSNGQDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGV 95

Query: 61  PFTNANVSLLRNFHIGLADTSPSSASQAFVSTDGVINARARVLGGGSAINAGFYTRASSR 120
           PFTNANVS LRNFHIGLAD S SSASQAFVSTDGV NARARVLGGGS INAGFY+RA + 
Sbjct: 96  PFTNANVSFLRNFHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAA 155

Query: 121 FIKKVGWDAKLVNESYSWVERQIVHRPELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTK 180
           F+K+ GWD KLV ESY WVER+IVH+P+L  WQKA  DSLL+VG+ PFNGFTYDH+ GTK
Sbjct: 156 FVKRAGWDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTK 215

Query: 181 VGGTIFDRFGRRHTTAELLASGNPDNLSVLVHATVQRLIFHSAHGKKPKAIGVVFKDETG 240
           +GGTIFDRFGRRHT AELLA  NP  L VL++ATVQ+++F ++ G +P+  GV+FKDE G
Sbjct: 216 IGGTIFDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTS-GTRPRVTGVIFKDEKG 275

Query: 241 NQNEVFLSSKRQSEVILSSGAIATPQMLLLSGIGPRADLEKWNISVVLDNEFVGKGMADN 300
           NQ++  LS+++ SEVILSSGAI +PQML+LSGIGP+ +L++  I VVL+NE VGKGMADN
Sbjct: 276 NQHQALLSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADN 335

Query: 301 PLNSIFVPSNRPVEQSLIQAVGITKLGVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTI 360
           P+N+I VPS  P+EQSLIQ VGITK+GVY+E+S+GFGQS ESIH H+G+MS +    STI
Sbjct: 336 PMNTILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTI 395

Query: 361 PPKQRTPEAIQAYIKNKQDLPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNY 420
           P KQR PEA QAYI   +    EAF G F+LEK+A PIS+G LSL+NTNVDDNP+VTFNY
Sbjct: 396 PAKQRRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNY 455

Query: 421 FGHPNDLHRCVEGVRMVTKIVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTK 480
           F HP                           C+++ + K+L++SVKANINL PK  NDTK
Sbjct: 456 FKHP---------------------------CDKQNVHKMLSLSVKANINLRPKQLNDTK 515

Query: 481 SLEQFCRDTVITIWHYHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMM 540
           S+ QFC+DTV+TIWHYHGGCLVGKVV+ +RKVLGV RLR++DGSTF ESPGTNPQAT+MM
Sbjct: 516 SMAQFCKDTVVTIWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMM 551

Query: 541 MGRY 543
           MGRY
Sbjct: 576 MGRY 551

BLAST of MS009564 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 532.7 bits (1371), Expect = 3.4e-151
Identity = 266/529 (50.28%), Postives = 351/529 (66.35%), Query Frame = 0

Query: 26  FDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPFTNANVSLLRNFHIGLADTSPSSASQ 85
           +DYII+GGGTAGCPLAATLSQ   VL+LERG  P+ N N++ L  F   L+D S SS SQ
Sbjct: 46  YDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNITRLSAFGAALSDLSESSPSQ 105

Query: 86  AFVSTDGVINARARVLGGGSAINAGFYTRASSRFIKKVGWDAKLVNESYSWVERQIVHRP 145
            FVS DGVINARARVLGGGSA+NAGFYTRA +++++ +GWD  L NESY WVE ++  +P
Sbjct: 106 RFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRNMGWDGALANESYQWVEAKVAFQP 165

Query: 146 ELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDNL 205
            +  WQ A  D LL+ GI P NGFTYDH+ GTK GGTIFDR G RHT A+LL   +P  +
Sbjct: 166 PMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGI 225

Query: 206 SVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQNEVFLSSKRQSEVILSSGAIATPQM 265
           +VL+HATV R++F +    KP A GVV++D TG  +  +L     SE+ILS+G + +PQ+
Sbjct: 226 TVLLHATVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQL 285

Query: 266 LLLSGIGPRADLEKWNISVVLDNEFVGKGMADNPLNSIFVPSNRPVEQSLIQAVGITKLG 325
           L+LSG+GP A L+  NI+VV+D   VG+GM DNP+N++FVPS  PVE SLI+ VGIT  G
Sbjct: 286 LMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEG 345

Query: 326 VYIESSSG--FG------QSEESIHCHHGLMSAEIGQLSTIPPKQRTPEAIQAYIKNKQD 385
            Y+E++ G  FG          S   ++ + S      +T+       +   A       
Sbjct: 346 TYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSPR----ATLLESNSMTKLSSA------- 405

Query: 386 LPREAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFGHPNDLHRCVEGVRMVTK 445
              + F+GGF+LEK+  P+S G L L   N  DNP VTFNYF HP+DL RCV G++ + +
Sbjct: 406 ---QPFQGGFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIER 465

Query: 446 IVHSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSL----EQFCRDTVITIWH 505
           +V SK F+ Y    + + + LLN++    +NL P  +    SL    E+FC+ TV TIWH
Sbjct: 466 VVQSKAFSRYKYA-DVSFEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWH 525

Query: 506 YHGGCLVGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMGRY 543
           YHGGC+VG+VV+ D KV+G+ RLR++D ST    PGTNPQATVMM+GRY
Sbjct: 526 YHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGRY 559

BLAST of MS009564 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 529.6 bits (1363), Expect = 2.9e-150
Identity = 262/523 (50.10%), Postives = 347/523 (66.35%), Query Frame = 0

Query: 26  FDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPFTNANVSLLRNFHIGLADTSPSSASQ 85
           FDYII+GGGT+GC LAATLSQ   VLVLERGG P+ N   + + NF   L++TSP S SQ
Sbjct: 46  FDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFATTLSNTSPKSWSQ 105

Query: 86  AFVSTDGVINARARVLGGGSAINAGFYTRASSRFIKKVGWDAKLVNESYSWVERQIVHRP 145
            F+S DGV N RARVLGGGS +NAGFYTRA   ++K+  W    V  +Y WVE+++  +P
Sbjct: 106 LFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKETEWKTDEVEAAYEWVEKKVAFQP 165

Query: 146 ELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDNL 205
            + GWQ AF D LL+ G  P+NGFTYDH+YGTK+GGTIFDR G RHT A+LL   NP N+
Sbjct: 166 PVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNI 225

Query: 206 SVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQNEVFLSSKRQSEVILSSGAIATPQM 265
            V +HA+V +++F +    +PKA GV+F+D  G  ++  L     +EVILS+GAI +PQ+
Sbjct: 226 VVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQL 285

Query: 266 LLLSGIGPRADLEKWNIS-VVLDNEFVGKGMADNPLNSIFVPSNRPVEQSLIQAVGITKL 325
           L+LSGIGP A L    I  +VLD+  VG+GM DNP+N+IF+PS  PVE SLIQ VGITK 
Sbjct: 286 LMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKF 345

Query: 326 GVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPPKQRT-----PEAIQAYIKNKQDLP 385
             YIE +SG   S          +   + ++ T      T      ++I  +      L 
Sbjct: 346 ESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLL 405

Query: 386 REAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFGHPNDLHRCVEGVRMVTKIV 445
               + G +L+KIA PIS+G L L NTN DDNP+V FNY+  P DL  CVEG+  + K++
Sbjct: 406 NATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVI 465

Query: 446 HSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSLEQFCRDTVITIWHYHGGCL 505
           +SK F+ + +  + T+  LL++ +    NL P+H     +L QFC DTV+TIWHYHGGC 
Sbjct: 466 NSKAFSKF-KYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQ 525

Query: 506 VGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMGRY 543
           VG+VV+ + +VLG+  LR++DGSTF +SPGTNPQATVMM+GRY
Sbjct: 526 VGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGRY 567

BLAST of MS009564 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 529.6 bits (1363), Expect = 2.9e-150
Identity = 262/523 (50.10%), Postives = 347/523 (66.35%), Query Frame = 0

Query: 26  FDYIIVGGGTAGCPLAATLSQKFRVLVLERGGVPFTNANVSLLRNFHIGLADTSPSSASQ 85
           FDYII+GGGT+GC LAATLSQ   VLVLERGG P+ N   + + NF   L++TSP S SQ
Sbjct: 13  FDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFATTLSNTSPKSWSQ 72

Query: 86  AFVSTDGVINARARVLGGGSAINAGFYTRASSRFIKKVGWDAKLVNESYSWVERQIVHRP 145
            F+S DGV N RARVLGGGS +NAGFYTRA   ++K+  W    V  +Y WVE+++  +P
Sbjct: 73  LFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKETEWKTDEVEAAYEWVEKKVAFQP 132

Query: 146 ELAGWQKAFTDSLLDVGISPFNGFTYDHLYGTKVGGTIFDRFGRRHTTAELLASGNPDNL 205
            + GWQ AF D LL+ G  P+NGFTYDH+YGTK+GGTIFDR G RHT A+LL   NP N+
Sbjct: 133 PVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNI 192

Query: 206 SVLVHATVQRLIFHSAHGKKPKAIGVVFKDETGNQNEVFLSSKRQSEVILSSGAIATPQM 265
            V +HA+V +++F +    +PKA GV+F+D  G  ++  L     +EVILS+GAI +PQ+
Sbjct: 193 VVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQL 252

Query: 266 LLLSGIGPRADLEKWNIS-VVLDNEFVGKGMADNPLNSIFVPSNRPVEQSLIQAVGITKL 325
           L+LSGIGP A L    I  +VLD+  VG+GM DNP+N+IF+PS  PVE SLIQ VGITK 
Sbjct: 253 LMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKF 312

Query: 326 GVYIESSSGFGQSEESIHCHHGLMSAEIGQLSTIPPKQRT-----PEAIQAYIKNKQDLP 385
             YIE +SG   S          +   + ++ T      T      ++I  +      L 
Sbjct: 313 ESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLL 372

Query: 386 REAFKGGFVLEKIANPISKGQLSLINTNVDDNPAVTFNYFGHPNDLHRCVEGVRMVTKIV 445
               + G +L+KIA PIS+G L L NTN DDNP+V FNY+  P DL  CVEG+  + K++
Sbjct: 373 NATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVI 432

Query: 446 HSKYFTNYTQCNEETLDKLLNISVKANINLIPKHTNDTKSLEQFCRDTVITIWHYHGGCL 505
           +SK F+ + +  + T+  LL++ +    NL P+H     +L QFC DTV+TIWHYHGGC 
Sbjct: 433 NSKAFSKF-KYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQ 492

Query: 506 VGKVVNHDRKVLGVTRLRIVDGSTFSESPGTNPQATVMMMGRY 543
           VG+VV+ + +VLG+  LR++DGSTF +SPGTNPQATVMM+GRY
Sbjct: 493 VGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGRY 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154219.10.0e+0099.82protein HOTHEAD-like [Momordica charantia][more]
XP_022953252.16.4e-28589.30protein HOTHEAD-like [Cucurbita moschata][more]
XP_008462353.11.4e-28489.85PREDICTED: protein HOTHEAD-like [Cucumis melo][more]
XP_038899986.11.9e-28489.85protein HOTHEAD-like [Benincasa hispida][more]
KAA0059432.13.2e-28489.67protein HOTHEAD-like [Cucumis melo var. makuwa] >TYK03893.1 protein HOTHEAD-like... [more]
Match NameE-valueIdentityDescription
Q9S7465.9e-22568.57Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1[more]
Q9SSM21.5e-12446.25(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
P527064.1e-10139.20(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K25.4e-10139.29(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527075.0e-9938.83(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DLE30.0e+0099.82protein HOTHEAD-like OS=Momordica charantia OX=3673 GN=LOC111021526 PE=3 SV=1[more]
A0A6J1GMR03.1e-28589.30protein HOTHEAD-like OS=Cucurbita moschata OX=3662 GN=LOC111455858 PE=3 SV=1[more]
A0A1S3CIA96.9e-28589.85protein HOTHEAD-like OS=Cucumis melo OX=3656 GN=LOC103500726 PE=3 SV=1[more]
A0A5A7UU551.5e-28489.67Protein HOTHEAD-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347... [more]
A0A6J1JWY55.8e-28488.75protein HOTHEAD-like OS=Cucurbita maxima OX=3661 GN=LOC111488603 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G72970.14.2e-22668.57Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.21.3e-21165.63Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.13.4e-15150.28Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.12.9e-15050.10Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.22.9e-15050.10Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 26..540
e-value: 9.2E-153
score: 511.7
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 24..541
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 395..540
e-value: 1.4E-26
score: 93.7
NoneNo IPR availableGENE3D3.30.410.40coord: 143..490
e-value: 9.2E-153
score: 511.7
NoneNo IPR availablePIRSRPIRSR000137-2PIRSR000137-2coord: 23..542
e-value: 4.3E-121
score: 403.3
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 1..542
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 295..497
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 26..299
e-value: 8.9E-30
score: 104.1
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 258..272
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 5..542
e-value: 3.6E-106
score: 354.2
IPR031206Protein Hothead-likePANTHERPTHR45968:SF5PROTEIN HOTHEADcoord: 1..542

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009564.1MS009564.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors