MS009536 (gene) Bitter gourd (TR) v1

Overview
NameMS009536
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein CHUP1, chloroplastic isoform X3
Locationscaffold813: 2000195 .. 2002661 (+)
RNA-Seq ExpressionMS009536
SyntenyMS009536
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAGAAGAGGAATTTAAAGCCTATATTATTAAAATTTGGGGTGGTTCTGGCTATTTCCTTTGCTGGTTTTCTCTATTCCCGCTTTAGAATCAGGAAGAAAAGACCTCGTCTGCCTCCTCCCTCGTCAAGTTCTTCAGGTTTTACAGTTAATCTTGTGTGCTTATTTGATTTCTTAAAATTTATTCTTAATTTCATCAATTCCATTTTAGCAGATCAGGGCAATAAAGTTGACTTGAGTAGAGGAAGAGGACCTAAACTTGATAATCAAGCAATAAAGGTAGCAACATCTGCCTCCTTTAATGTTATTCTTTTTGCAGCTGATGCCTATTTAAGTTTTCATAGGATAGATTTTACAGACTTGTCCCAGCTAAATTATGTTTGTACATTTTGGTAATATGAATAAGAACTTAGAATGTTCGAAAAGTTTAGACACCAGGTAGGTTTTATGAATTTGGCTGTGCAAATTTTGTGTAAATGCTAATACCTTGTGATGAAAGTATCTTATTAATGAAAAAACCGTTGGGACTGTTGACTGTTTTCCCTCAGCCTATGTCCTTGTGAATACAAAACCGATATGCGCAATGATTTGTTTTGAATATTTGTTGGTCTCTTAGGCTACTGTGGAGTTTAAATGACATAAGGTGCATGTTATGTATTACAGTCATTTCTAAGCCCATGTCGTCAATTGTCAGTTTGATTTCTTTCTCAGGAAGAGATGTATATACCAAAAGTTAATGTTGATGATTCAAATGTTGGTCTCTGCCCTAGCAGTAAGCGTAGTGTAGATAAAGATGGGTTGTTTCTCCCTGAGCTTCAGGAACTTGTCAAGGAATCTGATTTTCCTGCAGCGAATGCTGGGTTATCTCATGAGAAGAACGTTGAAGCATTGAGGTCGGGTCTTCAAACTCCGAAAGCATATAACAATTTTGAGACGGATGACTACGAACAAGAGATCAGGCACCTCAAAAGTAAGGTGAAAATGCTTCGAGAGAGGGAGAGGAACCTTGAGGTTCAGCTACTTGAGTATTATGGCCTGAAAGAGCAGGAAACTGCTGTCATGGAGCTCCAAAATAGGTTGAAGATTAACAATATGGAAGCCAAGCTTTTCACCCTCAAGATCGAGTCCCTTCAGGCGGATAATCGAAGATTAGAATCACAAGTTTCTGATCATGCAAAATCAGTGTCTGACCTTGAGGCTGCAAGAGCAAAAATTAAGTTTCTCAAGAAAAAACTCAGATATGAAGCAGAACAGAACAGGGGACAGATCTTAAATCTTCAGCAAAGAGTTGCTAAGCTGCACGATCAAGAATATAAGACAAATGAAAGTAATAAGGATGCCCGAATTAAGCTGAAAAGGATTGAAGATTTGGAGAAAGAGGTAGAGGACTTAAGAAACTCGAATTTGAGATTACAAATAGAAAATTCTGATCTGGCTCGGAGATTAGATGCTACCCAAGTTCTTGCAAATTCTATTTTGGAAGACCCAGAAGTAAGCCCTTCTTGCTGTAAATCTATCTTACTTTTTTTTTTCCCCAATCGTAAGGTGATGAAAAATTTATTGCGTTATCTATCTCATTCTCATCTTATTTGTGTAGAAAGAATCCCTGAAAGAAGAAAGGGAGCGTCTAGGACAAGAAAATGAAAATTTGATGAAGGAGATTGAGCAACTTCAAGCTCACCGGTGTGCAGATGTTGAAGAGCTAGTCTATCTTCGCTGGATTAATGCTTGCTTAAGATATGAGCTGCGAAATTATCAGCCCCGACCAGGGAAAACAGCGGCAAGAGATCTAAGCAAAACGTTAAGCCCCAAATCTGAGGAGAAAGCAAAGAAGCTCATACTCGAATATGCAAATACAGAAGGAATTGAAGGGAAGGGCATCAACATTATGGATTTTGATTCAGATCAATGGTCATCCTCCCAAGCTTCCTCTCTTACAGATCAGGATGATTCGTATGTTGATTTTCAAGCAACAACAAAACCAAGTTCAAACAAAATCAAATTCATAAGTAAACTCAGGAAACTCTTGAAGGGAAAAGATAGTCAACAAAACCAGGCTCTGTCAGCAGAAAAATCTGCTGCATCCATAGAAGATAGTGATTCTCCACGTTACAGTTCGAGTAATTCTACCGGCACCAATGCTACGAGAGCCGAGGGACAGGGTATCGGATCTGCAAATTCATCTCAGAGTTCGTCGAGACATTCAATGGATTTTCGCAGATTGAGTAGCCAAGAGTATGGAAAACCTGAAGACTCTGTTAGAAGGAACAGTGATGGTGGATACACTAACAAGAGACTTGTTTTAGGTAGCAACCGTATGAGCAACTCGCCATTTAAAACTCCTAGTCCGGATACAGAATCTTCTGAAAAGTCTGAGTTGATGAAATATGCTGAAGTTTTGAAAGACTCTCCGGGAGCAAAGAACCGGTCGCATAGGAAGGGTGCTGCATCCATTGATTCGTAC

mRNA sequence

ATGGAAGAGAAGAGGAATTTAAAGCCTATATTATTAAAATTTGGGGTGGTTCTGGCTATTTCCTTTGCTGGTTTTCTCTATTCCCGCTTTAGAATCAGGAAGAAAAGACCTCGTCTGCCTCCTCCCTCGTCAAGTTCTTCAGGTTTTACAGTTAATCTTGTGTGCTTATTTGATTTCTTAAAATTTATTCTTAATTTCATCAATTCCATTTTAGCAGATCAGGGCAATAAAGTTGACTTGAGTAGAGGAAGAGGACCTAAACTTGATAATCAAGCAATAAAGGTAGCAACATCTGCCTCCTTTAATGTTATTCTTTTTGCAGCTGATGCCTATTTAAAGATGTATATACCAAAAGTTAATGTTGATGATTCAAATGTTGGTCTCTGCCCTAGCAGTAAGCGTAGTGTAGATAAAGATGGGTTGTTTCTCCCTGAGCTTCAGGAACTTGTCAAGGAATCTGATTTTCCTGCAGCGAATGCTGGGTTATCTCATGAGAAGAACGTTGAAGCATTGAGGTCGGGTCTTCAAACTCCGAAAGCATATAACAATTTTGAGACGGATGACTACGAACAAGAGATCAGGCACCTCAAAAGTAAGGTGAAAATGCTTCGAGAGAGGGAGAGGAACCTTGAGGTTCAGCTACTTGAGTATTATGGCCTGAAAGAGCAGGAAACTGCTGTCATGGAGCTCCAAAATAGGTTGAAGATTAACAATATGGAAGCCAAGCTTTTCACCCTCAAGATCGAGTCCCTTCAGGCGGATAATCGAAGATTAGAATCACAAGTTTCTGATCATGCAAAATCAGTGTCTGACCTTGAGGCTGCAAGAGCAAAAATTAAGTTTCTCAAGAAAAAACTCAGATATGAAGCAGAACAGAACAGGGGACAGATCTTAAATCTTCAGCAAAGAGTTGCTAAGCTGCACGATCAAGAATATAAGACAAATGAAAGTAATAAGGATGCCCGAATTAAGCTGAAAAGGATTGAAGATTTGGAGAAAGAGGTAGAGGACTTAAGAAACTCGAATTTGAGATTACAAATAGAAAATTCTGATCTGGCTCGGAGATTAGATGCTACCCAAGTTCTTGCAAATTCTATTTTGGAAGACCCAGAAAAAGAATCCCTGAAAGAAGAAAGGGAGCGTCTAGGACAAGAAAATGAAAATTTGATGAAGGAGATTGAGCAACTTCAAGCTCACCGGTGTGCAGATGTTGAAGAGCTAGTCTATCTTCGCTGGATTAATGCTTGCTTAAGATATGAGCTGCGAAATTATCAGCCCCGACCAGGGAAAACAGCGGCAAGAGATCTAAGCAAAACGTTAAGCCCCAAATCTGAGGAGAAAGCAAAGAAGCTCATACTCGAATATGCAAATACAGAAGGAATTGAAGGGAAGGGCATCAACATTATGGATTTTGATTCAGATCAATGGTCATCCTCCCAAGCTTCCTCTCTTACAGATCAGGATGATTCGTATGTTGATTTTCAAGCAACAACAAAACCAAGTTCAAACAAAATCAAATTCATAAGTAAACTCAGGAAACTCTTGAAGGGAAAAGATAGTCAACAAAACCAGGCTCTGTCAGCAGAAAAATCTGCTGCATCCATAGAAGATAGTGATTCTCCACGTTACAGTTCGAGTAATTCTACCGGCACCAATGCTACGAGAGCCGAGGGACAGGGTATCGGATCTGCAAATTCATCTCAGAGTTCGTCGAGACATTCAATGGATTTTCGCAGATTGAGTAGCCAAGAGTATGGAAAACCTGAAGACTCTGTTAGAAGGAACAGTGATGGTGGATACACTAACAAGAGACTTGTTTTAGGTAGCAACCGTATGAGCAACTCGCCATTTAAAACTCCTAGTCCGGATACAGAATCTTCTGAAAAGTCTGAGTTGATGAAATATGCTGAAGTTTTGAAAGACTCTCCGGGAGCAAAGAACCGGTCGCATAGGAAGGGTGCTGCATCCATTGATTCGTAC

Coding sequence (CDS)

ATGGAAGAGAAGAGGAATTTAAAGCCTATATTATTAAAATTTGGGGTGGTTCTGGCTATTTCCTTTGCTGGTTTTCTCTATTCCCGCTTTAGAATCAGGAAGAAAAGACCTCGTCTGCCTCCTCCCTCGTCAAGTTCTTCAGGTTTTACAGTTAATCTTGTGTGCTTATTTGATTTCTTAAAATTTATTCTTAATTTCATCAATTCCATTTTAGCAGATCAGGGCAATAAAGTTGACTTGAGTAGAGGAAGAGGACCTAAACTTGATAATCAAGCAATAAAGGTAGCAACATCTGCCTCCTTTAATGTTATTCTTTTTGCAGCTGATGCCTATTTAAAGATGTATATACCAAAAGTTAATGTTGATGATTCAAATGTTGGTCTCTGCCCTAGCAGTAAGCGTAGTGTAGATAAAGATGGGTTGTTTCTCCCTGAGCTTCAGGAACTTGTCAAGGAATCTGATTTTCCTGCAGCGAATGCTGGGTTATCTCATGAGAAGAACGTTGAAGCATTGAGGTCGGGTCTTCAAACTCCGAAAGCATATAACAATTTTGAGACGGATGACTACGAACAAGAGATCAGGCACCTCAAAAGTAAGGTGAAAATGCTTCGAGAGAGGGAGAGGAACCTTGAGGTTCAGCTACTTGAGTATTATGGCCTGAAAGAGCAGGAAACTGCTGTCATGGAGCTCCAAAATAGGTTGAAGATTAACAATATGGAAGCCAAGCTTTTCACCCTCAAGATCGAGTCCCTTCAGGCGGATAATCGAAGATTAGAATCACAAGTTTCTGATCATGCAAAATCAGTGTCTGACCTTGAGGCTGCAAGAGCAAAAATTAAGTTTCTCAAGAAAAAACTCAGATATGAAGCAGAACAGAACAGGGGACAGATCTTAAATCTTCAGCAAAGAGTTGCTAAGCTGCACGATCAAGAATATAAGACAAATGAAAGTAATAAGGATGCCCGAATTAAGCTGAAAAGGATTGAAGATTTGGAGAAAGAGGTAGAGGACTTAAGAAACTCGAATTTGAGATTACAAATAGAAAATTCTGATCTGGCTCGGAGATTAGATGCTACCCAAGTTCTTGCAAATTCTATTTTGGAAGACCCAGAAAAAGAATCCCTGAAAGAAGAAAGGGAGCGTCTAGGACAAGAAAATGAAAATTTGATGAAGGAGATTGAGCAACTTCAAGCTCACCGGTGTGCAGATGTTGAAGAGCTAGTCTATCTTCGCTGGATTAATGCTTGCTTAAGATATGAGCTGCGAAATTATCAGCCCCGACCAGGGAAAACAGCGGCAAGAGATCTAAGCAAAACGTTAAGCCCCAAATCTGAGGAGAAAGCAAAGAAGCTCATACTCGAATATGCAAATACAGAAGGAATTGAAGGGAAGGGCATCAACATTATGGATTTTGATTCAGATCAATGGTCATCCTCCCAAGCTTCCTCTCTTACAGATCAGGATGATTCGTATGTTGATTTTCAAGCAACAACAAAACCAAGTTCAAACAAAATCAAATTCATAAGTAAACTCAGGAAACTCTTGAAGGGAAAAGATAGTCAACAAAACCAGGCTCTGTCAGCAGAAAAATCTGCTGCATCCATAGAAGATAGTGATTCTCCACGTTACAGTTCGAGTAATTCTACCGGCACCAATGCTACGAGAGCCGAGGGACAGGGTATCGGATCTGCAAATTCATCTCAGAGTTCGTCGAGACATTCAATGGATTTTCGCAGATTGAGTAGCCAAGAGTATGGAAAACCTGAAGACTCTGTTAGAAGGAACAGTGATGGTGGATACACTAACAAGAGACTTGTTTTAGGTAGCAACCGTATGAGCAACTCGCCATTTAAAACTCCTAGTCCGGATACAGAATCTTCTGAAAAGTCTGAGTTGATGAAATATGCTGAAGTTTTGAAAGACTCTCCGGGAGCAAAGAACCGGTCGCATAGGAAGGGTGCTGCATCCATTGATTCGTAC

Protein sequence

MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDFLKFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKVNVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKAYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNLQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQVLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIEDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAASIDSY
Homology
BLAST of MS009536 vs. NCBI nr
Match: XP_022147425.1 (protein CHUP1, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 616/660 (93.33%), Postives = 618/660 (93.64%), Query Frame = 0

Query: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDFL 60
           MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS             
Sbjct: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS------------- 60

Query: 61  KFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKVN 120
                      ADQGNKVDLSRGRGPKLDNQAIK                  +MYIPKVN
Sbjct: 61  -----------ADQGNKVDLSRGRGPKLDNQAIKE-----------------EMYIPKVN 120

Query: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180
           VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA
Sbjct: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180

Query: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240
           YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME
Sbjct: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240

Query: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300
           AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL
Sbjct: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300

Query: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360
           QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ
Sbjct: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360

Query: 361 VLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYE 420
           VLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYE
Sbjct: 361 VLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYE 420

Query: 421 LRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQ 480
           LRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQ
Sbjct: 421 LRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQ 480

Query: 481 ASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIEDSDS 540
           ASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAAS+EDSDS
Sbjct: 481 ASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASMEDSDS 540

Query: 541 PRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRNSDGGY 600
           PRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRNSDGGY
Sbjct: 541 PRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRNSDGGY 600

Query: 601 TNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAASIDSY 660
           TNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAASIDSY
Sbjct: 601 TNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAASIDSY 619

BLAST of MS009536 vs. NCBI nr
Match: XP_022147409.1 (protein CHUP1, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 616/665 (92.63%), Postives = 618/665 (92.93%), Query Frame = 0

Query: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDFL 60
           MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS             
Sbjct: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS------------- 60

Query: 61  KFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKVN 120
                      ADQGNKVDLSRGRGPKLDNQAIK                  +MYIPKVN
Sbjct: 61  -----------ADQGNKVDLSRGRGPKLDNQAIKE-----------------EMYIPKVN 120

Query: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180
           VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA
Sbjct: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180

Query: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240
           YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME
Sbjct: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240

Query: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300
           AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL
Sbjct: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300

Query: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360
           QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ
Sbjct: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360

Query: 361 VLANSILEDPE-----KESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA 420
           VLANSILEDPE     KESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA
Sbjct: 361 VLANSILEDPEVTLLAKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA 420

Query: 421 CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ 480
           CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ
Sbjct: 421 CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ 480

Query: 481 WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASI 540
           WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAAS+
Sbjct: 481 WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASM 540

Query: 541 EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN 600
           EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN
Sbjct: 541 EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN 600

Query: 601 SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA 660
           SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA
Sbjct: 601 SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA 624

BLAST of MS009536 vs. NCBI nr
Match: XP_022147417.1 (protein CHUP1, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 615/665 (92.48%), Postives = 617/665 (92.78%), Query Frame = 0

Query: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDFL 60
           MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS             
Sbjct: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS------------- 60

Query: 61  KFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKVN 120
                       DQGNKVDLSRGRGPKLDNQAIK                  +MYIPKVN
Sbjct: 61  ------------DQGNKVDLSRGRGPKLDNQAIKE-----------------EMYIPKVN 120

Query: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180
           VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA
Sbjct: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180

Query: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240
           YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME
Sbjct: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240

Query: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300
           AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL
Sbjct: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300

Query: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360
           QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ
Sbjct: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360

Query: 361 VLANSILEDPE-----KESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA 420
           VLANSILEDPE     KESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA
Sbjct: 361 VLANSILEDPEVTLLAKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA 420

Query: 421 CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ 480
           CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ
Sbjct: 421 CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ 480

Query: 481 WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASI 540
           WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAAS+
Sbjct: 481 WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASM 540

Query: 541 EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN 600
           EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN
Sbjct: 541 EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN 600

Query: 601 SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA 660
           SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA
Sbjct: 601 SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA 623

BLAST of MS009536 vs. NCBI nr
Match: XP_022147434.1 (protein CHUP1, chloroplastic isoform X4 [Momordica charantia])

HSP 1 Score: 1006.9 bits (2602), Expect = 8.0e-290
Identity = 546/552 (98.91%), Postives = 547/552 (99.09%), Query Frame = 0

Query: 114 MYIPKVNVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRS 173
           MYIPKVNVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRS
Sbjct: 1   MYIPKVNVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRS 60

Query: 174 GLQTPKAYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNR 233
           GLQTPKAYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNR
Sbjct: 61  GLQTPKAYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNR 120

Query: 234 LKINNMEAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQN 293
           LKINNMEAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQN
Sbjct: 121 LKINNMEAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQN 180

Query: 294 RGQILNLQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLA 353
           RGQILNLQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLA
Sbjct: 181 RGQILNLQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLA 240

Query: 354 RRLDATQVLANSILEDPE-----KESLKEERERLGQENENLMKEIEQLQAHRCADVEELV 413
           RRLDATQVLANSILEDPE     KESLKEERERLGQENENLMKEIEQLQAHRCADVEELV
Sbjct: 241 RRLDATQVLANSILEDPEVTLLAKESLKEERERLGQENENLMKEIEQLQAHRCADVEELV 300

Query: 414 YLRWINACLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINI 473
           YLRWINACLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINI
Sbjct: 301 YLRWINACLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINI 360

Query: 474 MDFDSDQWSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSA 533
           MDFDSDQWSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSA
Sbjct: 361 MDFDSDQWSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSA 420

Query: 534 EKSAASIEDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKP 593
           EKSAAS+EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKP
Sbjct: 421 EKSAASMEDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKP 480

Query: 594 EDSVRRNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNR 653
           EDSVRRNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNR
Sbjct: 481 EDSVRRNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNR 540

Query: 654 SHRKGAASIDSY 661
           SHRKGAASIDSY
Sbjct: 541 SHRKGAASIDSY 552

BLAST of MS009536 vs. NCBI nr
Match: XP_038898688.1 (protein CHUP1, chloroplastic [Benincasa hispida] >XP_038898689.1 protein CHUP1, chloroplastic [Benincasa hispida] >XP_038898690.1 protein CHUP1, chloroplastic [Benincasa hispida])

HSP 1 Score: 892.1 bits (2304), Expect = 2.9e-255
Identity = 508/667 (76.16%), Postives = 557/667 (83.51%), Query Frame = 0

Query: 1   MEEKRNL-KPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDF 60
           M++KR+L KPIL KFG  LAISFAGFL S+FR+R KRP L PPSSSSS            
Sbjct: 1   MDDKRDLMKPILFKFGFALAISFAGFLCSQFRLRNKRPPLLPPSSSSSD----------- 60

Query: 61  LKFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKV 120
                        DQ +KVDL RGRGP+LDNQ +K AT+AS NV+ FA DAY K  IPKV
Sbjct: 61  -------------DQSSKVDLGRGRGPRLDNQGLKAATAASSNVVHFAVDAYEKKCIPKV 120

Query: 121 NVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPK 180
           N DDSN+GL PS+K  VDKDGL LPE QELVKE DF AANAGL  +KNVEA RSGL+TPK
Sbjct: 121 NFDDSNIGLRPSNKHGVDKDGL-LPEFQELVKEFDFSAANAGLPPKKNVEAPRSGLETPK 180

Query: 181 AYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNM 240
           AY   E D+YEQEIRHLKSKVK LRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNM
Sbjct: 181 AYKTVEDDEYEQEIRHLKSKVKTLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNM 240

Query: 241 EAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILN 300
           EAKLFTLKIESLQADNRRLESQV DHAKSVSDLEAA+AKIKFLKKK+RYEAEQNRGQILN
Sbjct: 241 EAKLFTLKIESLQADNRRLESQVCDHAKSVSDLEAAKAKIKFLKKKIRYEAEQNRGQILN 300

Query: 301 LQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDAT 360
           LQQRV KL DQE+KTNESNKDA+I+L++IE+LEKE+EDLR SNL+LQIENSDL+RRLDAT
Sbjct: 301 LQQRVVKLQDQEHKTNESNKDAQIRLQKIEELEKEIEDLRKSNLKLQIENSDLSRRLDAT 360

Query: 361 QVLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRY 420
           Q LANS+LED EKESLKEE ERL +ENE L KEIEQLQAHRCAD+EELVYLRWINACLRY
Sbjct: 361 QFLANSLLEDQEKESLKEEMERLSRENEALTKEIEQLQAHRCADIEELVYLRWINACLRY 420

Query: 421 ELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSS 480
           ELRN+QP  GKTAARDLSKTLSPKSEEKAKKLIL+YANTEGIEGK INI DFDSDQWSSS
Sbjct: 421 ELRNFQPPAGKTAARDLSKTLSPKSEEKAKKLILDYANTEGIEGKSINITDFDSDQWSSS 480

Query: 481 QASSLT---DQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIE 540
           QASS T   D DDS VDF +T K SSNK+KFISKLRKLL+GK SQQN  L AEKSAAS+E
Sbjct: 481 QASSHTDPGDPDDSAVDFPSTAKTSSNKVKFISKLRKLLRGKGSQQNLTLLAEKSAASVE 540

Query: 541 DSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQ--EYGKPEDSV-R 600
           DS SPRYSSSNS GTNATRAEGQGIG    S++SSRHSMDF RL+SQ  + GK EDS+ R
Sbjct: 541 DSGSPRYSSSNSPGTNATRAEGQGIGYTTPSRNSSRHSMDFHRLNSQKEDDGKTEDSIRR 600

Query: 601 RNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKG 660
           RNSD GY NK+ VLGS+  SNS +++ S DTES+EKSELMKYAEVLKD+ GAKN+S RK 
Sbjct: 601 RNSDVGYVNKKFVLGSDESSNSSYRSQSQDTESTEKSELMKYAEVLKDTRGAKNQSQRK- 641

BLAST of MS009536 vs. ExPASy Swiss-Prot
Match: Q9LI74 (Protein CHUP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHUP1 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 3.0e-53
Identity = 167/456 (36.62%), Postives = 258/456 (56.58%), Query Frame = 0

Query: 139 DGLFLPELQELVK-ESDFPAANAGLSHEKNVEALRSGLQTPKAYNNFETDDYEQEIRHLK 198
           D   LPE ++L+  E ++P  +   + EK  +  +   +   AYN       + E+  LK
Sbjct: 85  DDDILPEFEDLLSGEIEYPLPDDDNNLEKAEKERK--YEVEMAYN-------DGELERLK 144

Query: 199 SKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRR 258
             VK L ERE  LE +LLEYYGLKEQE+ ++ELQ +LKI  +E  +  + I SLQA+ ++
Sbjct: 145 QLVKELEEREVKLEGELLEYYGLKEQESDIVELQRQLKIKTVEIDMLNITINSLQAERKK 204

Query: 259 LESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNLQQRVAKLHDQEYKTNES 318
           L+ ++S +     +LE AR KIK L+++++ +A Q +GQ+L L+Q V+ L  +E +    
Sbjct: 205 LQEELSQNGIVRKELEVARNKIKELQRQIQLDANQTKGQLLLLKQHVSSLQMKEEEAMNK 264

Query: 319 NKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ---VLANSILEDPEKES 378
           + +   KLK ++DLE +V +L+  N  LQ E  +L+ +LD+ +      +++ E  +   
Sbjct: 265 DTEVERKLKAVQDLEVQVMELKRKNRELQHEKRELSIKLDSAEARIATLSNMTESDKVAK 324

Query: 379 LKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYELRNYQPRPGKTAAR 438
           ++EE   L   NE+L+K++E LQ +R ++VEELVYLRW+NACLRYELRNYQ   GK +AR
Sbjct: 325 VREEVNNLKHNNEDLLKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYQTPAGKISAR 384

Query: 439 DLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQASSLTDQDDSYVDF 498
           DLSK LSPKS+ KAK+L+LEYA +E    +G    D +S+ +S   +    D D++ +D 
Sbjct: 385 DLSKNLSPKSQAKAKRLMLEYAGSE----RGQGDTDLESN-YSQPSSPGSDDFDNASMDS 444

Query: 499 QATTKPS-SNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIEDSDSPRYSSSNSTGTNA 558
             +   S S K   I KL+K  K KD    Q+      + S       R SSS     N 
Sbjct: 445 STSRFSSFSKKPGLIQKLKKWGKSKDDSSVQS----SPSRSFYGGSPGRLSSS----MNK 504

Query: 559 TRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPE 590
            R   + +   N+ +S +     F ++  +  G PE
Sbjct: 505 QRGPLESLMIRNAGESVA--ITTFGQVDQESPGTPE 516

BLAST of MS009536 vs. ExPASy Swiss-Prot
Match: Q3V6T2 (Girdin OS=Homo sapiens OX=9606 GN=CCDC88A PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 1.5e-04
Identity = 58/217 (26.73%), Postives = 117/217 (53.92%), Query Frame = 0

Query: 187 DDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNR-LKINNMEAKLFT 246
           ++ E E+ HL+ + ++L+++  NL++   +   L EQE + +E +NR LK      K  T
Sbjct: 628 EELENELHHLEKENELLQKKITNLKITCEKIEAL-EQENSELERENRKLKKTLDSFKNLT 687

Query: 247 LKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLK---KKLRYEAEQNRGQILNLQQ 306
            ++ESL+ +N +L+ +  +  ++V  L+ A  K+  L+   K+L  E EQ +  +  L+ 
Sbjct: 688 FQLESLEKENSQLDEENLELRRNVESLKCASMKMAQLQLENKELESEKEQLKKGLELLKA 747

Query: 307 RVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQVL 366
              K    E      + + +   K +E+  K+++ L +    L++EN  L + L+  ++ 
Sbjct: 748 SFKKTERLEVSYQGLDIENQRLQKTLENSNKKIQQLESELQDLEMENQTLQKNLEELKI- 807

Query: 367 ANSILEDPEKE--SLKEERERLGQENENLMKEIEQLQ 398
           ++  LE  EKE  SL++E  +L ++ + L KE ++L+
Sbjct: 808 SSKRLEQLEKENKSLEQETSQLEKDKKQLEKENKRLR 842

BLAST of MS009536 vs. ExPASy Swiss-Prot
Match: Q5SNZ0 (Girdin OS=Mus musculus OX=10090 GN=Ccdc88a PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 2.6e-04
Identity = 56/217 (25.81%), Postives = 116/217 (53.46%), Query Frame = 0

Query: 187 DDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEA-KLFT 246
           ++ E E+ HL  + ++L+++  NL++   E     EQE + +E +NR     +++ K  T
Sbjct: 628 EELENELNHLGKENELLQKKITNLKI-TCEKLETLEQENSELERENRKFKKTLDSFKNLT 687

Query: 247 LKIESLQADNRRLESQVSDHAKSVSDLEAA---RAKIKFLKKKLRYEAEQNRGQILNLQQ 306
            ++ESL+ +N +L+ +  +  +SV  L+ A    A+++   K+L  E EQ R  +  ++ 
Sbjct: 688 FQLESLEKENSQLDEENLELRRSVESLKCASMRMAQLQLENKELESEKEQLRKGLELMRA 747

Query: 307 RVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQVL 366
              K    E      + + +   K +E+  K+++ L +    L++EN  L + L+  ++ 
Sbjct: 748 SFKKTERLEVSYQGLDTENQRLQKALENSNKKIQQLESELQDLEMENQTLQKSLEELKI- 807

Query: 367 ANSILEDPEKE--SLKEERERLGQENENLMKEIEQLQ 398
           ++  LE  EKE  SL++E  +L ++ + L KE ++L+
Sbjct: 808 SSKRLEQLEKENKSLEQETSQLEKDKKQLEKENKRLR 842

BLAST of MS009536 vs. ExPASy TrEMBL
Match: A0A6J1D049 (protein CHUP1, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016343 PE=4 SV=1)

HSP 1 Score: 1126.7 bits (2913), Expect = 0.0e+00
Identity = 616/660 (93.33%), Postives = 618/660 (93.64%), Query Frame = 0

Query: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDFL 60
           MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS             
Sbjct: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS------------- 60

Query: 61  KFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKVN 120
                      ADQGNKVDLSRGRGPKLDNQAIK                  +MYIPKVN
Sbjct: 61  -----------ADQGNKVDLSRGRGPKLDNQAIKE-----------------EMYIPKVN 120

Query: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180
           VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA
Sbjct: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180

Query: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240
           YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME
Sbjct: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240

Query: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300
           AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL
Sbjct: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300

Query: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360
           QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ
Sbjct: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360

Query: 361 VLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYE 420
           VLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYE
Sbjct: 361 VLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYE 420

Query: 421 LRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQ 480
           LRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQ
Sbjct: 421 LRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQ 480

Query: 481 ASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIEDSDS 540
           ASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAAS+EDSDS
Sbjct: 481 ASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASMEDSDS 540

Query: 541 PRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRNSDGGY 600
           PRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRNSDGGY
Sbjct: 541 PRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRNSDGGY 600

Query: 601 TNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAASIDSY 660
           TNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAASIDSY
Sbjct: 601 TNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAASIDSY 619

BLAST of MS009536 vs. ExPASy TrEMBL
Match: A0A6J1D0X5 (protein CHUP1, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016343 PE=4 SV=1)

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 616/665 (92.63%), Postives = 618/665 (92.93%), Query Frame = 0

Query: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDFL 60
           MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS             
Sbjct: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS------------- 60

Query: 61  KFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKVN 120
                      ADQGNKVDLSRGRGPKLDNQAIK                  +MYIPKVN
Sbjct: 61  -----------ADQGNKVDLSRGRGPKLDNQAIKE-----------------EMYIPKVN 120

Query: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180
           VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA
Sbjct: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180

Query: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240
           YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME
Sbjct: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240

Query: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300
           AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL
Sbjct: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300

Query: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360
           QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ
Sbjct: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360

Query: 361 VLANSILEDPE-----KESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA 420
           VLANSILEDPE     KESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA
Sbjct: 361 VLANSILEDPEVTLLAKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA 420

Query: 421 CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ 480
           CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ
Sbjct: 421 CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ 480

Query: 481 WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASI 540
           WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAAS+
Sbjct: 481 WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASM 540

Query: 541 EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN 600
           EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN
Sbjct: 541 EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN 600

Query: 601 SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA 660
           SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA
Sbjct: 601 SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA 624

BLAST of MS009536 vs. ExPASy TrEMBL
Match: A0A6J1D2B1 (protein CHUP1, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016343 PE=4 SV=1)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 615/665 (92.48%), Postives = 617/665 (92.78%), Query Frame = 0

Query: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDFL 60
           MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS             
Sbjct: 1   MEEKRNLKPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSS------------- 60

Query: 61  KFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKVN 120
                       DQGNKVDLSRGRGPKLDNQAIK                  +MYIPKVN
Sbjct: 61  ------------DQGNKVDLSRGRGPKLDNQAIKE-----------------EMYIPKVN 120

Query: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180
           VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA
Sbjct: 121 VDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPKA 180

Query: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240
           YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME
Sbjct: 181 YNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNME 240

Query: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300
           AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL
Sbjct: 241 AKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNL 300

Query: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360
           QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ
Sbjct: 301 QQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ 360

Query: 361 VLANSILEDPE-----KESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA 420
           VLANSILEDPE     KESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA
Sbjct: 361 VLANSILEDPEVTLLAKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINA 420

Query: 421 CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ 480
           CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ
Sbjct: 421 CLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQ 480

Query: 481 WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASI 540
           WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAAS+
Sbjct: 481 WSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASM 540

Query: 541 EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN 600
           EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN
Sbjct: 541 EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDSVRRN 600

Query: 601 SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA 660
           SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA
Sbjct: 601 SDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGAA 623

BLAST of MS009536 vs. ExPASy TrEMBL
Match: A0A6J1D100 (protein CHUP1, chloroplastic isoform X4 OS=Momordica charantia OX=3673 GN=LOC111016343 PE=4 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.9e-290
Identity = 546/552 (98.91%), Postives = 547/552 (99.09%), Query Frame = 0

Query: 114 MYIPKVNVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRS 173
           MYIPKVNVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRS
Sbjct: 1   MYIPKVNVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRS 60

Query: 174 GLQTPKAYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNR 233
           GLQTPKAYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNR
Sbjct: 61  GLQTPKAYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNR 120

Query: 234 LKINNMEAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQN 293
           LKINNMEAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQN
Sbjct: 121 LKINNMEAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQN 180

Query: 294 RGQILNLQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLA 353
           RGQILNLQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLA
Sbjct: 181 RGQILNLQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLA 240

Query: 354 RRLDATQVLANSILEDPE-----KESLKEERERLGQENENLMKEIEQLQAHRCADVEELV 413
           RRLDATQVLANSILEDPE     KESLKEERERLGQENENLMKEIEQLQAHRCADVEELV
Sbjct: 241 RRLDATQVLANSILEDPEVTLLAKESLKEERERLGQENENLMKEIEQLQAHRCADVEELV 300

Query: 414 YLRWINACLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINI 473
           YLRWINACLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINI
Sbjct: 301 YLRWINACLRYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINI 360

Query: 474 MDFDSDQWSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSA 533
           MDFDSDQWSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSA
Sbjct: 361 MDFDSDQWSSSQASSLTDQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSA 420

Query: 534 EKSAASIEDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKP 593
           EKSAAS+EDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKP
Sbjct: 421 EKSAASMEDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKP 480

Query: 594 EDSVRRNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNR 653
           EDSVRRNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNR
Sbjct: 481 EDSVRRNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNR 540

Query: 654 SHRKGAASIDSY 661
           SHRKGAASIDSY
Sbjct: 541 SHRKGAASIDSY 552

BLAST of MS009536 vs. ExPASy TrEMBL
Match: A0A5A7V182 (Protein CHUP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00460 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 4.5e-254
Identity = 505/666 (75.83%), Postives = 554/666 (83.18%), Query Frame = 0

Query: 1   MEEKRNL-KPILLKFGVVLAISFAGFLYSRFRIRKKRPRLPPPSSSSSGFTVNLVCLFDF 60
           ME+K NL KP+LLKFGVVLAISFA FLYSRFR++ KRP LPPP SSSS            
Sbjct: 1   MEDKGNLMKPLLLKFGVVLAISFASFLYSRFRLKNKRPPLPPPLSSSSD----------- 60

Query: 61  LKFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKV 120
                        DQGNKV+L RGRGP+LDNQ +K AT+AS NV+LFA DAY +M IPKV
Sbjct: 61  -------------DQGNKVNLGRGRGPRLDNQGMKAATAASSNVVLFAVDAYEEMCIPKV 120

Query: 121 NVDDSNVGLCPSSKRSVDKDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTPK 180
           NVDDSN+GLCPS+K  VDKDGL LPE QE VKE D  AANA  S +KNVEA RSGL+TPK
Sbjct: 121 NVDDSNLGLCPSNKHGVDKDGLLLPEFQEHVKEFDLSAANAEFSPKKNVEAPRSGLETPK 180

Query: 181 AYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNM 240
           AY   E D+YEQEIRHLKSKVKMLRERERNLE QLLEYYGLKEQETAVMELQNRLKINNM
Sbjct: 181 AYKTVEDDEYEQEIRHLKSKVKMLRERERNLEFQLLEYYGLKEQETAVMELQNRLKINNM 240

Query: 241 EAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILN 300
           EAKLFT KIESL+ADNRRLESQV +HAK+VSDLEAARAKIKFLKKKLR+EAEQNR QILN
Sbjct: 241 EAKLFTFKIESLEADNRRLESQVCNHAKTVSDLEAARAKIKFLKKKLRHEAEQNRRQILN 300

Query: 301 LQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDAT 360
           LQQ+V KL DQE+KTNESNKDA+IKL++IEDLEKE+E+LR  N RLQIENSDL RRLDAT
Sbjct: 301 LQQKVLKLQDQEHKTNESNKDAQIKLQKIEDLEKEIEELRKLNSRLQIENSDLGRRLDAT 360

Query: 361 QVLANSILEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRY 420
           Q LANS+LED EKESLKEE ERL QENE L KEIEQLQAHR ADVEELVYLRWINACLRY
Sbjct: 361 QFLANSLLEDQEKESLKEETERLTQENEALTKEIEQLQAHRLADVEELVYLRWINACLRY 420

Query: 421 ELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSS 480
           ELRN+QP  GKTAARDLSKTLSPKSEEKAKKLIL+YANTEG EGKGI++ DFDSDQWSSS
Sbjct: 421 ELRNFQPPAGKTAARDLSKTLSPKSEEKAKKLILDYANTEGNEGKGISVTDFDSDQWSSS 480

Query: 481 QASSLT---DQDDSYVDFQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIE 540
           QASS T   D DDS  +F +T K SSNKIKFI KL+KLL+GK SQQN  L AEKSAASIE
Sbjct: 481 QASSHTDPGDPDDSAAEFPSTAKTSSNKIKFIGKLKKLLRGKGSQQNLTLLAEKSAASIE 540

Query: 541 DSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYG--KPEDSVRR 600
           DSDSP YSSSNSTGTNATRAEGQ IG A SS++SSR+S+DF+RL SQ+    K EDS RR
Sbjct: 541 DSDSPCYSSSNSTGTNATRAEGQAIGYATSSRNSSRYSIDFQRLHSQKEDEVKTEDSARR 600

Query: 601 NSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHRKGA 660
           NSD GY NKR VLGS++ SNS  ++ S DTES+EKSELMKYAEVLKD+ GAKN+SHRK A
Sbjct: 601 NSDVGYVNKRFVLGSDQSSNSSDRSQSQDTESTEKSELMKYAEVLKDTRGAKNQSHRK-A 641

BLAST of MS009536 vs. TAIR 10
Match: AT1G52080.1 (actin binding protein family )

HSP 1 Score: 336.3 bits (861), Expect = 5.7e-92
Identity = 255/664 (38.40%), Postives = 370/664 (55.72%), Query Frame = 0

Query: 4   KRNLKPILLKFGVVLAISFAGFLYSRFRIRKKR--PRLP--PPSSSSSGFTVNLVCLFDF 63
           KR++  ++L+ G  LA+SFAGFL++RFR   KR  P LP  PP SS +G+          
Sbjct: 7   KRDINLLVLQLGAALAVSFAGFLFARFRKNTKRIGPTLPPLPPHSSDNGY---------- 66

Query: 64  LKFILNFINSILADQGNKVDLSRGRGPKLDNQAIKVATSASFNVILFAADAYLKMYIPKV 123
                        D  NK    R  G +  +                             
Sbjct: 67  ------------RDYSNKSIDRRDEGTEKTD----------------------------- 126

Query: 124 NVDDSNVGLCPSSKRSVD-KDGLFLPELQELVKESDFPAANAGLSHEKNVEALRSGLQTP 183
             +++ +G+ P  +  +D KD   LPE +E  K+ D    +       + E  RS +  P
Sbjct: 127 --EETLIGVSPRRECDLDEKDVFLLPEFEEEAKKLDLLVCD-------DCETPRSDITAP 186

Query: 184 KAYNNFETDDYEQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINN 243
            A+ + E  D+E EI  L++ V+ LRERER LE +LLEYY LKEQ+   MEL++RLK+N 
Sbjct: 187 LAFPSEEEADHENEINRLRNTVRALRERERCLEDKLLEYYSLKEQQKIAMELRSRLKLNQ 246

Query: 244 MEAKLFTLKIESLQADNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQIL 303
           ME K+F  KI+ LQA+N +L+++  +H+K + +L+ A+++++ LKKKL    +Q+  QIL
Sbjct: 247 METKVFNFKIKKLQAENEKLKAECFEHSKVLLELDMAKSQVQVLKKKLNINTQQHVAQIL 306

Query: 304 NLQQRVAKLHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDA 363
           +L+QRVA+L ++E K    + +A   ++R+ DLE E+ +L ++N RLQ EN +L+ +L++
Sbjct: 307 SLKQRVARLQEEEIKAVLPDLEADKMMQRLRDLESEINELTDTNTRLQFENFELSEKLES 366

Query: 364 TQVLANSILEDPEK-ESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACL 423
            Q++ANS LE+PE+ E+L+E+  RL  ENE L K++EQLQ  RC D+E+LVYLRWINACL
Sbjct: 367 VQIIANSKLEEPEEIETLREDCNRLRSENEELKKDVEQLQGDRCTDLEQLVYLRWINACL 426

Query: 424 RYELRNYQPRPGKTAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWS 483
           RYELR YQP  GKT ARDLS TLSP SEEKAK+LILEYA++E          + D D+WS
Sbjct: 427 RYELRTYQPPAGKTVARDLSTTLSPTSEEKAKQLILEYAHSED---------NTDYDRWS 486

Query: 484 SSQ--ASSLTDQ---DDSYVD-FQATTKPSSNKIKFISKLRKLLKGKDSQQNQALSAEKS 543
           SSQ  +S +TD    DDS VD   AT    + K K + KL K+L GKD++      ++K 
Sbjct: 487 SSQEESSMITDSMFLDDSSVDTLFATKTKKTGKKKLMHKLMKILHGKDTK-----DSKKR 546

Query: 544 AASIEDSDSPRYSSSNSTGTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPEDS 603
           A S E        SS++TG ++T             Q  S HSMDF+ L     GK E+ 
Sbjct: 547 AGSSE-------PSSSNTGVHST-----------PRQLRSTHSMDFQMLMR---GKDEEE 570

Query: 604 VRRNSDGGYTNKRLVLGSNRMSNSPFKTPSPDTESSEKSELMKYAEVLKDSPGAKNRSHR 656
             +N       K    GS+       +    +T+ + K EL+K A+ L  S   K + H+
Sbjct: 607 DFKNHIVMLRRKSEAAGSSTYG----EEHCLETDQNGKKELIKLADALTKSRSTK-KLHK 570

BLAST of MS009536 vs. TAIR 10
Match: AT3G25690.1 (Hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 211.5 bits (537), Expect = 2.1e-54
Identity = 167/456 (36.62%), Postives = 258/456 (56.58%), Query Frame = 0

Query: 139 DGLFLPELQELVK-ESDFPAANAGLSHEKNVEALRSGLQTPKAYNNFETDDYEQEIRHLK 198
           D   LPE ++L+  E ++P  +   + EK  +  +   +   AYN       + E+  LK
Sbjct: 85  DDDILPEFEDLLSGEIEYPLPDDDNNLEKAEKERK--YEVEMAYN-------DGELERLK 144

Query: 199 SKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRR 258
             VK L ERE  LE +LLEYYGLKEQE+ ++ELQ +LKI  +E  +  + I SLQA+ ++
Sbjct: 145 QLVKELEEREVKLEGELLEYYGLKEQESDIVELQRQLKIKTVEIDMLNITINSLQAERKK 204

Query: 259 LESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNLQQRVAKLHDQEYKTNES 318
           L+ ++S +     +LE AR KIK L+++++ +A Q +GQ+L L+Q V+ L  +E +    
Sbjct: 205 LQEELSQNGIVRKELEVARNKIKELQRQIQLDANQTKGQLLLLKQHVSSLQMKEEEAMNK 264

Query: 319 NKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ---VLANSILEDPEKES 378
           + +   KLK ++DLE +V +L+  N  LQ E  +L+ +LD+ +      +++ E  +   
Sbjct: 265 DTEVERKLKAVQDLEVQVMELKRKNRELQHEKRELSIKLDSAEARIATLSNMTESDKVAK 324

Query: 379 LKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYELRNYQPRPGKTAAR 438
           ++EE   L   NE+L+K++E LQ +R ++VEELVYLRW+NACLRYELRNYQ   GK +AR
Sbjct: 325 VREEVNNLKHNNEDLLKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYQTPAGKISAR 384

Query: 439 DLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQASSLTDQDDSYVDF 498
           DLSK LSPKS+ KAK+L+LEYA +E    +G    D +S+ +S   +    D D++ +D 
Sbjct: 385 DLSKNLSPKSQAKAKRLMLEYAGSE----RGQGDTDLESN-YSQPSSPGSDDFDNASMDS 444

Query: 499 QATTKPS-SNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIEDSDSPRYSSSNSTGTNA 558
             +   S S K   I KL+K  K KD    Q+      + S       R SSS     N 
Sbjct: 445 STSRFSSFSKKPGLIQKLKKWGKSKDDSSVQS----SPSRSFYGGSPGRLSSS----MNK 504

Query: 559 TRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPE 590
            R   + +   N+ +S +     F ++  +  G PE
Sbjct: 505 QRGPLESLMIRNAGESVA--ITTFGQVDQESPGTPE 516

BLAST of MS009536 vs. TAIR 10
Match: AT3G25690.2 (Hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 211.5 bits (537), Expect = 2.1e-54
Identity = 167/456 (36.62%), Postives = 258/456 (56.58%), Query Frame = 0

Query: 139 DGLFLPELQELVK-ESDFPAANAGLSHEKNVEALRSGLQTPKAYNNFETDDYEQEIRHLK 198
           D   LPE ++L+  E ++P  +   + EK  +  +   +   AYN       + E+  LK
Sbjct: 85  DDDILPEFEDLLSGEIEYPLPDDDNNLEKAEKERK--YEVEMAYN-------DGELERLK 144

Query: 199 SKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIESLQADNRR 258
             VK L ERE  LE +LLEYYGLKEQE+ ++ELQ +LKI  +E  +  + I SLQA+ ++
Sbjct: 145 QLVKELEEREVKLEGELLEYYGLKEQESDIVELQRQLKIKTVEIDMLNITINSLQAERKK 204

Query: 259 LESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNLQQRVAKLHDQEYKTNES 318
           L+ ++S +     +LE AR KIK L+++++ +A Q +GQ+L L+Q V+ L  +E +    
Sbjct: 205 LQEELSQNGIVRKELEVARNKIKELQRQIQLDANQTKGQLLLLKQHVSSLQMKEEEAMNK 264

Query: 319 NKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ---VLANSILEDPEKES 378
           + +   KLK ++DLE +V +L+  N  LQ E  +L+ +LD+ +      +++ E  +   
Sbjct: 265 DTEVERKLKAVQDLEVQVMELKRKNRELQHEKRELSIKLDSAEARIATLSNMTESDKVAK 324

Query: 379 LKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYELRNYQPRPGKTAAR 438
           ++EE   L   NE+L+K++E LQ +R ++VEELVYLRW+NACLRYELRNYQ   GK +AR
Sbjct: 325 VREEVNNLKHNNEDLLKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYQTPAGKISAR 384

Query: 439 DLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQASSLTDQDDSYVDF 498
           DLSK LSPKS+ KAK+L+LEYA +E    +G    D +S+ +S   +    D D++ +D 
Sbjct: 385 DLSKNLSPKSQAKAKRLMLEYAGSE----RGQGDTDLESN-YSQPSSPGSDDFDNASMDS 444

Query: 499 QATTKPS-SNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIEDSDSPRYSSSNSTGTNA 558
             +   S S K   I KL+K  K KD    Q+      + S       R SSS     N 
Sbjct: 445 STSRFSSFSKKPGLIQKLKKWGKSKDDSSVQS----SPSRSFYGGSPGRLSSS----MNK 504

Query: 559 TRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPE 590
            R   + +   N+ +S +     F ++  +  G PE
Sbjct: 505 QRGPLESLMIRNAGESVA--ITTFGQVDQESPGTPE 516

BLAST of MS009536 vs. TAIR 10
Match: AT3G25690.3 (Hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 149.8 bits (377), Expect = 7.5e-36
Identity = 121/340 (35.59%), Postives = 192/340 (56.47%), Query Frame = 0

Query: 254 DNRRLESQVSDHAKSVSDLEAARAKIKFLKKKLRYEAEQNRGQILNLQQRVAKLHDQEYK 313
           +++ L+ ++S +     +LE AR KIK L+++++ +A Q +GQ+L L+Q V+ L  +E +
Sbjct: 51  NDKNLQEELSQNGIVRKELEVARNKIKELQRQIQLDANQTKGQLLLLKQHVSSLQMKEEE 110

Query: 314 TNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQ---VLANSILEDP 373
               + +   KLK ++DLE +V +L+  N  LQ E  +L+ +LD+ +      +++ E  
Sbjct: 111 AMNKDTEVERKLKAVQDLEVQVMELKRKNRELQHEKRELSIKLDSAEARIATLSNMTESD 170

Query: 374 EKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYELRNYQPRPGK 433
           +   ++EE   L   NE+L+K++E LQ +R ++VEELVYLRW+NACLRYELRNYQ   GK
Sbjct: 171 KVAKVREEVNNLKHNNEDLLKQVEGLQMNRFSEVEELVYLRWVNACLRYELRNYQTPAGK 230

Query: 434 TAARDLSKTLSPKSEEKAKKLILEYANTEGIEGKGINIMDFDSDQWSSSQASSLTDQDDS 493
            +ARDLSK LSPKS+ KAK+L+LEYA +E    +G    D +S+ +S   +    D D++
Sbjct: 231 ISARDLSKNLSPKSQAKAKRLMLEYAGSE----RGQGDTDLESN-YSQPSSPGSDDFDNA 290

Query: 494 YVDFQATTKPS-SNKIKFISKLRKLLKGKDSQQNQALSAEKSAASIEDSDSPRYSSSNST 553
            +D   +   S S K   I KL+K  K KD    Q+      + S       R SSS   
Sbjct: 291 SMDSSTSRFSSFSKKPGLIQKLKKWGKSKDDSSVQS----SPSRSFYGGSPGRLSSS--- 350

Query: 554 GTNATRAEGQGIGSANSSQSSSRHSMDFRRLSSQEYGKPE 590
             N  R   + +   N+ +S +     F ++  +  G PE
Sbjct: 351 -MNKQRGPLESLMIRNAGESVA--ITTFGQVDQESPGTPE 375

BLAST of MS009536 vs. TAIR 10
Match: AT2G36650.1 (unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 50.4 bits (119), Expect = 6.2e-06
Identity = 56/235 (23.83%), Postives = 121/235 (51.49%), Query Frame = 0

Query: 190 EQEIRHLKSKVKMLRERERNLEVQLLEYYGLKEQETAVMELQNRLKINNMEAKLFTLKIE 249
           +QEI  LKS+ + L+ +E  +E+    +  LK+QE  ++E ++ L +   +   F  ++ 
Sbjct: 75  KQEILSLKSRFEELQRKEYEMELHFERFCNLKDQEVMLIEHKSILSLEKAQLDFFRKEVL 134

Query: 250 SLQADNRRLESQVSDHAKSVSDLEAARAKIKFLK---KKLRYEAEQNRGQILNLQQRVAK 309
           +++ +++R ++ V  + K V +++  R++   L+   KKLR +++Q   +++N  +++  
Sbjct: 135 AMEEEHKRGQALVIVYLKLVGEIQELRSENGLLEGKAKKLRRKSKQ-LYRVVNESRKIIG 194

Query: 310 LHDQEYKTNESNKDARIKLKRIEDLEKEVEDLRNSNLRLQIENSDLARRLDATQVLANSI 369
           +  +  K  +   +   K   +++LE +V+D+      LQ E  +L            S 
Sbjct: 195 VEKEFLKCVD---ELETKNNIVKELEGKVKDMEAYVDVLQEEKEEL---------FMKSS 254

Query: 370 LEDPEKESLKEERERLGQENENLMKEIEQLQAHRCADVEELVYLRWINACLRYEL 422
               E  S+++ R         +++E E+L+      V+E++ LRW NACLR+E+
Sbjct: 255 NSTSEMVSVEDYR--------RIVEEYEELKKDYANGVKEVINLRWSNACLRHEV 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147425.10.0e+0093.33protein CHUP1, chloroplastic isoform X3 [Momordica charantia][more]
XP_022147409.10.0e+0092.63protein CHUP1, chloroplastic isoform X1 [Momordica charantia][more]
XP_022147417.10.0e+0092.48protein CHUP1, chloroplastic isoform X2 [Momordica charantia][more]
XP_022147434.18.0e-29098.91protein CHUP1, chloroplastic isoform X4 [Momordica charantia][more]
XP_038898688.12.9e-25576.16protein CHUP1, chloroplastic [Benincasa hispida] >XP_038898689.1 protein CHUP1, ... [more]
Match NameE-valueIdentityDescription
Q9LI743.0e-5336.62Protein CHUP1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHUP1 PE=1 SV=1[more]
Q3V6T21.5e-0426.73Girdin OS=Homo sapiens OX=9606 GN=CCDC88A PE=1 SV=2[more]
Q5SNZ02.6e-0425.81Girdin OS=Mus musculus OX=10090 GN=Ccdc88a PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1D0490.0e+0093.33protein CHUP1, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1D0X50.0e+0092.63protein CHUP1, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1D2B10.0e+0092.48protein CHUP1, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1D1003.9e-29098.91protein CHUP1, chloroplastic isoform X4 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A5A7V1824.5e-25475.83Protein CHUP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00460 ... [more]
Match NameE-valueIdentityDescription
AT1G52080.15.7e-9238.40actin binding protein family [more]
AT3G25690.12.1e-5436.62Hydroxyproline-rich glycoprotein family protein [more]
AT3G25690.22.1e-5436.62Hydroxyproline-rich glycoprotein family protein [more]
AT3G25690.37.5e-3635.59Hydroxyproline-rich glycoprotein family protein [more]
AT2G36650.16.2e-0623.83unknown protein; Has 35333 Blast hits to 34131 proteins in 2444 species: Archae ... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 186..213
NoneNo IPR availableCOILSCoilCoilcoord: 220..310
NoneNo IPR availableCOILSCoilCoilcoord: 318..355
NoneNo IPR availableCOILSCoilCoilcoord: 372..399
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 577..597
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 602..623
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 518..660
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 624..660
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 518..576
NoneNo IPR availablePANTHERPTHR31342:SF4ACTIN BINDING PROTEIN FAMILYcoord: 73..654
coord: 2..47
IPR040265Protein CHUP1-likePANTHERPTHR31342PROTEIN CHUP1, CHLOROPLASTICcoord: 2..47
IPR040265Protein CHUP1-likePANTHERPTHR31342PROTEIN CHUP1, CHLOROPLASTICcoord: 73..654

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009536.1MS009536.1mRNA