MS009501 (gene) Bitter gourd (TR) v1

Overview


Name	MS009501
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Location	scaffold813: 1767018 .. 1768205 (-)
RNA-Seq Expression	MS009501
Synteny	MS009501

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGGCTGGAAACCCTAATTGGTGGGCCATATTTCCACCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCTTTTTCCCCCTCAGTTTCCTTCTAATTTCTCTGATCACCTTCACCCTCACTCATGGAGCCAACTCCTCTTGTAAGTGTGCAATTAATCAATTTTAATTTGCATGTTTTTTTTCTTCTCTTTCTTATAATTATTACCCTTTTTTCAGAGGTGATGAAGATAGGGTTATTAATAACCAAACCCAGATGGGTCATTATCAGCCAAAAAAGTTGGAGAATTGGGAGGACCAAATATTGAATAATAACCATAATCAAGCCCCTAATTTTGTTGATATTGTTAAGCAAGAAGTCTCCAACAACAGGGATCAGTCTCTAATTCTTCAGCCTGACTCTACTTTAACTTCTTCTTCTTCTCACTCCAACAAATTCCTAAACTTTTCTTACAATAATTCCAACCCAGATCACAACATCAATCAATCTGCCTCACAACACTCCCCAGAGGTGAGAATTTTAATATTACTGCCAAAATTATATTTCCTGCCTTCTCTCCTTCTAACTCTCTCTCTCTGAATAGTCTAACCGGTCCGCCGCCTCCGGTGGAGCATGTAAGAAGGCTAGGATTCACCCGCCGTCTTCCAGCCAGCCACCTCTGAAGGTACACCCGACTAGTCTAAGCATAATCTAAGCATAGTTCAGTTGTTAATATATATTTAAAGGGTATCACTTAAACATGTGGGAATAAACATTTTTGCATGAAAGTTTGCATCTTTTTCTTATGATTGTATTTTATGGTGATCCTAAGTTTGAATATGGCATAGCAGGTGAGGAAGGAGAAACTTGTGGATCGAATAACCGCTCTTCACCAAATTGTTTCCCCATTTGGAAAGGTAAAGTTTATTTTTGGTACTGTTTAACACTAAAAAACGAAAACGATTCGTAATTCAACTGGTTAAGACATATATTTTTGCATTTAGTTAAACCCTCTAATTTGTTTTTTCAAACAATCAAAAGGATTGCAGGCTTTAAATGCAAGCAGCTGGTTTTGATGATTTTTTTTTCTTTTTTTTTTTTGTATCCTTTTCTCTACCCTTTTGCAGACTGATACTGCCTCTGTGTTGTCAGAAGCTATTGGGTATATCAGATTTCTTCAGGGACAAATTGAGGTGATGCCAATTTTA

mRNA sequence

ATGATGGCTGGAAACCCTAATTGGTGGGCCATATTTCCACCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCTTTTTCCCCCTCAGTTTCCTTCTAATTTCTCTGATCACCTTCACCCTCACTCATGGAGCCAACTCCTCTTAGGTGATGAAGATAGGGTTATTAATAACCAAACCCAGATGGGTCATTATCAGCCAAAAAAGTTGGAGAATTGGGAGGACCAAATATTGAATAATAACCATAATCAAGCCCCTAATTTTGTTGATATTGTTAAGCAAGAAGTCTCCAACAACAGGGATCAGTCTCTAATTCTTCAGCCTGACTCTACTTTAACTTCTTCTTCTTCTCACTCCAACAAATTCCTAAACTTTTCTTACAATAATTCCAACCCAGATCACAACATCAATCAATCTGCCTCACAACACTCCCCAGAGTCTAACCGGTCCGCCGCCTCCGGTGGAGCATGTAAGAAGGCTAGGATTCACCCGCCGTCTTCCAGCCAGCCACCTCTGAAGGTGAGGAAGGAGAAACTTGTGGATCGAATAACCGCTCTTCACCAAATTGTTTCCCCATTTGGAAAGACTGATACTGCCTCTGTGTTGTCAGAAGCTATTGGGTATATCAGATTTCTTCAGGGACAAATTGAGGTGATGCCAATTTTA

Coding sequence (CDS)

ATGATGGCTGGAAACCCTAATTGGTGGGCCATATTTCCACCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCTTTTTCCCCCTCAGTTTCCTTCTAATTTCTCTGATCACCTTCACCCTCACTCATGGAGCCAACTCCTCTTAGGTGATGAAGATAGGGTTATTAATAACCAAACCCAGATGGGTCATTATCAGCCAAAAAAGTTGGAGAATTGGGAGGACCAAATATTGAATAATAACCATAATCAAGCCCCTAATTTTGTTGATATTGTTAAGCAAGAAGTCTCCAACAACAGGGATCAGTCTCTAATTCTTCAGCCTGACTCTACTTTAACTTCTTCTTCTTCTCACTCCAACAAATTCCTAAACTTTTCTTACAATAATTCCAACCCAGATCACAACATCAATCAATCTGCCTCACAACACTCCCCAGAGTCTAACCGGTCCGCCGCCTCCGGTGGAGCATGTAAGAAGGCTAGGATTCACCCGCCGTCTTCCAGCCAGCCACCTCTGAAGGTGAGGAAGGAGAAACTTGTGGATCGAATAACCGCTCTTCACCAAATTGTTTCCCCATTTGGAAAGACTGATACTGCCTCTGTGTTGTCAGAAGCTATTGGGTATATCAGATTTCTTCAGGGACAAATTGAGGTGATGCCAATTTTA

Protein sequence

MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQMGHYQPKKLENWEDQILNNNHNQAPNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNKFLNFSYNNSNPDHNINQSASQHSPESNRSAASGGACKKARIHPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVMPIL

Homology

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BLAST of MS009501 vs. NCBI nr
Match: XP_022146101.1 (transcription factor bHLH68-like [Momordica charantia])

HSP 1 Score: 412.5 bits (1059), Expect = 2.2e-111
Identity = 210/218 (96.33%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWWAIFPP  SSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ
Sbjct: 1   MMAGNPNWWAIFPP--SSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNNHNQAPNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNK 120
           MGHYQPK LENWEDQILNNNHNQ+PNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNK
Sbjct: 61  MGHYQPKNLENWEDQILNNNHNQSPNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNK 120

Query: 121 FLNFSYNNSNPDHNINQSASQHSPESNRSAASGGACKKARIHPPSSSQPPLKVRKEKLVD 180
           FLNF+YNNSNPDHNINQSASQHSPESNRSAASGGACKKAR+HPPSSSQPPLKVRKEKLVD
Sbjct: 121 FLNFTYNNSNPDHNINQSASQHSPESNRSAASGGACKKARVHPPSSSQPPLKVRKEKLVD 180

Query: 181 RITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVM 219
           RITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE +
Sbjct: 181 RITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEAL 216

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BLAST of MS009501 vs. NCBI nr
Match: XP_038897153.1 (LOW QUALITY PROTEIN: transcription factor bHLH133-like [Benincasa hispida])

HSP 1 Score: 259.2 bits (661), Expect = 3.2e-65
Identity = 157/235 (66.81%), Postives = 172/235 (73.19%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWW +FPPS +S               F+D   P SWSQLLLGDEDRV+ NQTQ
Sbjct: 1   MMAGNPNWWPMFPPSFNS---------------FAD--QPQSWSQLLLGDEDRVL-NQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNNHNQAP------NFVDIVKQEVSNN-RDQSL-ILQPDSTLT 120
           M HYQPKKLENWEDQILNNN+  +P      NFVDIVKQEVSNN RD SL IL P     
Sbjct: 61  MSHYQPKKLENWEDQILNNNNINSPSQVEASNFVDIVKQEVSNNIRDHSLNILHP---TL 120

Query: 121 SSSSHSNKFLNFSYNNSNPDHNINQSA-----SQHSPE----SNRSAASGGACKKARIHP 180
           ++S H+NKFL+FS N +N DH  NQS      S HS E    S  + A GGACKKA++HP
Sbjct: 121 NTSHHTNKFLDFSCNTNNQDHTTNQSTPRGADSDHSSELKNHSGPAGAVGGACKKAKVHP 180

Query: 181 PSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVM 219
           PSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE +
Sbjct: 181 PSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEAL 214

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BLAST of MS009501 vs. NCBI nr
Match: TYK04014.1 (transcription factor bHLH68-like [Cucumis melo var. makuwa])

HSP 1 Score: 248.8 bits (634), Expect = 4.3e-62
Identity = 152/235 (64.68%), Postives = 172/235 (73.19%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWWA+FPPSS +S              F+DH  P SWSQLLLGDEDRV+ NQTQ
Sbjct: 1   MMAGNPNWWAMFPPSSFNS--------------FADH-QPQSWSQLLLGDEDRVL-NQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNNHN-----QAPNFVD--IVKQEVSNNRDQSLILQPDSTLTS 120
           M HYQPKKLENWEDQILNNN+      +A NFVD  IVKQ++ +  +   IL P S    
Sbjct: 61  MSHYQPKKLENWEDQILNNNNTSSSQFEASNFVDNIIVKQQLHSTNNN--ILHPTSI--- 120

Query: 121 SSSHSNKFLNF-SYNNSNPDHNINQSA-------SQHSPESNRS----AASGGACKKARI 180
           SS H+NKFL+F S+ N+N DHN N  A         HS ES+++     ++GGACKKAR+
Sbjct: 121 SSHHTNKFLDFSSHTNNNQDHNTNHLALRAAGPDPDHSSESSKNHSVGGSTGGACKKARV 180

Query: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE 217
           HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE
Sbjct: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE 214

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BLAST of MS009501 vs. NCBI nr
Match: XP_004141839.1 (transcription factor bHLH68 [Cucumis sativus] >KGN45389.1 hypothetical protein Csa_016279 [Cucumis sativus])

HSP 1 Score: 245.0 bits (624), Expect = 6.2e-61
Identity = 151/237 (63.71%), Postives = 172/237 (72.57%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWWA+FPPSS SS              F+DH  P SWSQLLLGDEDRV+ NQTQ
Sbjct: 1   MMAGNPNWWAMFPPSSFSS--------------FADH-QPQSWSQLLLGDEDRVL-NQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNN-----HNQAPNFVD--IVKQEVSNNRDQSLILQPDSTLTS 120
           M HYQPKKLENWEDQILNNN       +A  FVD  IVKQ+  ++ +   IL P++ +  
Sbjct: 61  MSHYQPKKLENWEDQILNNNITSSSQFEASTFVDNIIVKQQQLHSNNN--ILHPNTII-- 120

Query: 121 SSSHSNKFLNFS--YNNSNPDHNINQSA-------SQHSPESNRS---AASGGACKKARI 180
           SS H+NKFL+FS   NN+N DHN N  A         H  ES+++    ++GGACKKAR+
Sbjct: 121 SSHHTNKFLDFSSNTNNNNQDHNTNHLALRPAAPDPDHLSESSKNQSIGSTGGACKKARV 180

Query: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVM 219
           HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE +
Sbjct: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEAL 217

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BLAST of MS009501 vs. NCBI nr
Match: XP_008465633.2 (PREDICTED: transcription factor bHLH68-like, partial [Cucumis melo])

HSP 1 Score: 241.1 bits (614), Expect = 8.9e-60
Identity = 148/231 (64.07%), Postives = 168/231 (72.73%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWWA+FPPSS +S              F+DH  P SWSQLLLGDEDRV+ NQTQ
Sbjct: 1   MMAGNPNWWAMFPPSSFNS--------------FADH-QPQSWSQLLLGDEDRVL-NQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNNHN-----QAPNFVD--IVKQEVSNNRDQSLILQPDSTLTS 120
           M HYQPKKLENWEDQILNNN+      +A NFVD  IVKQ++ +  +   IL P S    
Sbjct: 61  MSHYQPKKLENWEDQILNNNNTSSSQFEASNFVDNIIVKQQLHSTNNN--ILHPTSI--- 120

Query: 121 SSSHSNKFLNF-SYNNSNPDHNINQSA-------SQHSPESNRS----AASGGACKKARI 180
           SS H+NKFL+F S+ N+N DHN N  A         HS ES+++     ++GGACKKAR+
Sbjct: 121 SSHHTNKFLDFSSHTNNNQDHNTNHLALRAAGPDPDHSSESSKNHSVGGSTGGACKKARV 180

Query: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQ 213
           HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQ
Sbjct: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQ 210

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BLAST of MS009501 vs. ExPASy Swiss-Prot
Match: Q8S3D1 (Transcription factor bHLH68 OS=Arabidopsis thaliana OX=3702 GN=BHLH68 PE=1 SV=2)

HSP 1 Score: 124.4 bits (311), Expect = 1.6e-27
Identity = 116/300 (38.67%), Postives = 154/300 (51.33%), Query Frame = 0


Query: 1   MMAGNPNWWAI-------------------------------------FP-----PSSSS 60
           M AGNPNWW +                                     FP     P++SS
Sbjct: 15  MAAGNPNWWNVSGGMRPPPPLMGHQQAPLPPHMTPNNNYLRPRMMPTPFPHFLPSPATSS 74

Query: 61  SSSSSSLFPPQFPSN-------FSDHLHPHSWS-------QLLLGDEDRV-----INNQT 120
           SSSSSS   P  P+N        S+ L P SWS        L++G+E+R+      N+  
Sbjct: 75  SSSSSS---PSLPNNPNLSSWLESNDLPPESWSLSQLLLGGLMMGEEERLEMMNHHNHHD 134

Query: 121 QMGH--YQPK-KLENWEDQILNNNHNQAPNFVDIVKQEVS-NNRDQSLILQPDS------ 180
           +  H  +Q K +LENWE+Q+L  +H QA      +KQE + NN +  +I  P+S      
Sbjct: 135 EQQHHGFQGKIRLENWEEQVL--SHQQASMVAVDIKQEGNINNNNGYVISSPNSPPNKSC 194

Query: 181 -TLTSSSSHSNKFLNFSYNNS----NPDHN-INQSASQHSP-----ESNRSAASGGACKK 219
            T T+++S ++   N + NN+    + +HN ++ S  +H+P     E N     G   KK
Sbjct: 195 VTTTTTTSLNSNDDNINNNNNMLDFSSNHNGLHLSEGRHTPPDRSSECNSLEIGGSTNKK 254

[Close]

BLAST of MS009501 vs. ExPASy Swiss-Prot
Match: Q7XHI5 (Transcription factor bHLH133 OS=Arabidopsis thaliana OX=3702 GN=BHLH133 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-26
Identity = 105/268 (39.18%), Postives = 137/268 (51.12%), Query Frame = 0


Query: 3   AGNPNWW-----AIFPPS-------------------------SSSSSSSSSLFPPQFPS 62
           AGNPNWW      + PP+                         +SSSSSS S  PP    
Sbjct: 17  AGNPNWWNNVSRGLRPPTPLMSHEPPSTTAFIPSLLPNFFSSPTSSSSSSPSFPPPNSNP 76

Query: 63  NFSDHL--------HPHSWSQLLL-----GDEDRV-----INNQTQMGHYQPKKLENWED 122
           NFS  L         P S SQLLL     G+E+++      ++Q Q   YQ K+++NWE+
Sbjct: 77  NFSSWLEMSDLPLDQPWSLSQLLLGGLMMGEEEKMEMMNHHHHQNQHQSYQAKRIQNWEE 136

Query: 123 QILNNNHNQAPNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNKFLNFSYNNSNPDHN 182
           Q+L +  +        +KQE SNN    ++  P+S    S +        +  N+N D+N
Sbjct: 137 QVLRHQAS--------MKQESSNNNSYGIMSSPNSPPNKSCA--------TIINTNEDNN 196

Query: 183 INQSASQHSPESNRSAASGG--ACKKARIHPPSSSQPPLKVRKEKLVDRITALHQIVSPF 221
            N  +  +  E N S   G   A KK ++  P SSQ  LKVRKEKL  RI +LHQ+VSPF
Sbjct: 197 NNIHSGLNLSECNSSEMIGSSFANKKPKLQVP-SSQSTLKVRKEKLGGRIASLHQLVSPF 256

[Close]

BLAST of MS009501 vs. ExPASy Swiss-Prot
Match: Q9SFZ3 (Transcription factor bHLH110 OS=Arabidopsis thaliana OX=3702 GN=BHLH110 PE=2 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.0e-14
Identity = 52/100 (52.00%), Postives = 65/100 (65.00%), Query Frame = 0


Query: 128 NSNPDHNINQSASQ-HSPESNR------SAASGGACKKARIHPPSSSQPPLKVRKEKLVD 187
           +S+P H +   +++  + E  R      + A   A KK R+   SS  PP KVRKEKL D
Sbjct: 282 SSSPTHQMEMFSNEPQTSEGKRHNFLMATKAGENASKKPRVESRSSC-PPFKVRKEKLGD 341

Query: 188 RITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVMPI 221
           RI AL Q+VSPFGKTDTASVL EAIGYI+FLQ QIE + +
Sbjct: 342 RIAALQQLVSPFGKTDTASVLMEAIGYIKFLQSQIETLSV 380

[Close]

BLAST of MS009501 vs. ExPASy Swiss-Prot
Match: Q8VZ22 (Transcription factor bHLH103 OS=Arabidopsis thaliana OX=3702 GN=BHLH103 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 41/59 (69.49%), Postives = 46/59 (77.97%), Query Frame = 0


Query: 157 KKARIHPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQI 216
           K+ R+  PS   P  KVRKEKL DRITAL Q+VSPFGKTDTASVL +AI YI+FLQ QI
Sbjct: 176 KRPRLETPSHF-PSFKVRKEKLGDRITALQQLVSPFGKTDTASVLHDAIDYIKFLQEQI 233

[Close]

BLAST of MS009501 vs. ExPASy Swiss-Prot
Match: Q9LT67 (Transcription factor bHLH113 OS=Arabidopsis thaliana OX=3702 GN=BHLH113 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.0e-11
Identity = 51/130 (39.23%), Postives = 72/130 (55.38%), Query Frame = 0


Query: 98  NRDQSLILQPDSTLTSSSSHSNKFLNFSYNNSNPDHNINQSASQ---------HSPESNR 157
           N D+SL L+P    +  S      +     NSN    +++S            +  ES++
Sbjct: 74  NDDESLFLEPSVPTSGVSDLDPSCIKIDCRNSNDACTVDKSTKSSTKKRTGTGNGQESDQ 133

Query: 158 SAASGGACKKARIHPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYI 217
           +   G   KK + +   SS    KVRKE+L +RI AL Q+VSP+GKTD ASVL EA+GYI
Sbjct: 134 NRKPG---KKGKRNQEKSSVGIAKVRKERLGERIAALQQLVSPYGKTDAASVLHEAMGYI 193

Query: 218 RFLQGQIEVM 219
           +FLQ QI+V+
Sbjct: 194 KFLQDQIQVL 200

[Close]

BLAST of MS009501 vs. ExPASy TrEMBL
Match: A0A6J1CYC1 (transcription factor bHLH68-like OS=Momordica charantia OX=3673 GN=LOC111015393 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.1e-111
Identity = 210/218 (96.33%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWWAIFPP  SSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ
Sbjct: 1   MMAGNPNWWAIFPP--SSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNNHNQAPNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNK 120
           MGHYQPK LENWEDQILNNNHNQ+PNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNK
Sbjct: 61  MGHYQPKNLENWEDQILNNNHNQSPNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNK 120

Query: 121 FLNFSYNNSNPDHNINQSASQHSPESNRSAASGGACKKARIHPPSSSQPPLKVRKEKLVD 180
           FLNF+YNNSNPDHNINQSASQHSPESNRSAASGGACKKAR+HPPSSSQPPLKVRKEKLVD
Sbjct: 121 FLNFTYNNSNPDHNINQSASQHSPESNRSAASGGACKKARVHPPSSSQPPLKVRKEKLVD 180

Query: 181 RITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVM 219
           RITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE +
Sbjct: 181 RITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEAL 216

[Close]

BLAST of MS009501 vs. ExPASy TrEMBL
Match: A0A5D3BYE2 (Transcription factor bHLH68-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002120 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.1e-62
Identity = 152/235 (64.68%), Postives = 172/235 (73.19%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWWA+FPPSS +S              F+DH  P SWSQLLLGDEDRV+ NQTQ
Sbjct: 1   MMAGNPNWWAMFPPSSFNS--------------FADH-QPQSWSQLLLGDEDRVL-NQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNNHN-----QAPNFVD--IVKQEVSNNRDQSLILQPDSTLTS 120
           M HYQPKKLENWEDQILNNN+      +A NFVD  IVKQ++ +  +   IL P S    
Sbjct: 61  MSHYQPKKLENWEDQILNNNNTSSSQFEASNFVDNIIVKQQLHSTNNN--ILHPTSI--- 120

Query: 121 SSSHSNKFLNF-SYNNSNPDHNINQSA-------SQHSPESNRS----AASGGACKKARI 180
           SS H+NKFL+F S+ N+N DHN N  A         HS ES+++     ++GGACKKAR+
Sbjct: 121 SSHHTNKFLDFSSHTNNNQDHNTNHLALRAAGPDPDHSSESSKNHSVGGSTGGACKKARV 180

Query: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE 217
           HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE
Sbjct: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE 214

[Close]

BLAST of MS009501 vs. ExPASy TrEMBL
Match: A0A0A0K957 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447040 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 3.0e-61
Identity = 151/237 (63.71%), Postives = 172/237 (72.57%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWWA+FPPSS SS              F+DH  P SWSQLLLGDEDRV+ NQTQ
Sbjct: 1   MMAGNPNWWAMFPPSSFSS--------------FADH-QPQSWSQLLLGDEDRVL-NQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNN-----HNQAPNFVD--IVKQEVSNNRDQSLILQPDSTLTS 120
           M HYQPKKLENWEDQILNNN       +A  FVD  IVKQ+  ++ +   IL P++ +  
Sbjct: 61  MSHYQPKKLENWEDQILNNNITSSSQFEASTFVDNIIVKQQQLHSNNN--ILHPNTII-- 120

Query: 121 SSSHSNKFLNFS--YNNSNPDHNINQSA-------SQHSPESNRS---AASGGACKKARI 180
           SS H+NKFL+FS   NN+N DHN N  A         H  ES+++    ++GGACKKAR+
Sbjct: 121 SSHHTNKFLDFSSNTNNNNQDHNTNHLALRPAAPDPDHLSESSKNQSIGSTGGACKKARV 180

Query: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVM 219
           HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE +
Sbjct: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEAL 217

[Close]

BLAST of MS009501 vs. ExPASy TrEMBL
Match: A0A1S3CQT4 (transcription factor bHLH68-like OS=Cucumis melo OX=3656 GN=LOC103503272 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 4.3e-60
Identity = 148/231 (64.07%), Postives = 168/231 (72.73%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWWA+FPPSS +S              F+DH  P SWSQLLLGDEDRV+ NQTQ
Sbjct: 1   MMAGNPNWWAMFPPSSFNS--------------FADH-QPQSWSQLLLGDEDRVL-NQTQ 60

Query: 61  MGHYQPKKLENWEDQILNNNHN-----QAPNFVD--IVKQEVSNNRDQSLILQPDSTLTS 120
           M HYQPKKLENWEDQILNNN+      +A NFVD  IVKQ++ +  +   IL P S    
Sbjct: 61  MSHYQPKKLENWEDQILNNNNTSSSQFEASNFVDNIIVKQQLHSTNNN--ILHPTSI--- 120

Query: 121 SSSHSNKFLNF-SYNNSNPDHNINQSA-------SQHSPESNRS----AASGGACKKARI 180
           SS H+NKFL+F S+ N+N DHN N  A         HS ES+++     ++GGACKKAR+
Sbjct: 121 SSHHTNKFLDFSSHTNNNQDHNTNHLALRAAGPDPDHSSESSKNHSVGGSTGGACKKARV 180

Query: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQ 213
           HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQ
Sbjct: 181 HPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQ 210

[Close]

BLAST of MS009501 vs. ExPASy TrEMBL
Match: A0A6J1HGP4 (transcription factor bHLH68-like OS=Cucurbita moschata OX=3662 GN=LOC111464167 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 3.2e-55
Identity = 141/238 (59.24%), Postives = 155/238 (65.13%), Query Frame = 0


Query: 1   MMAGNPNWWAIFPPSSSSSSSSSSLFPPQFPSNFSDHLHPHSWSQLLLGDEDRVINNQTQ 60
           MMAGNPNWW +F PS SS +                   P SWSQLLLG+E+RV+ NQTQ
Sbjct: 1   MMAGNPNWWGMFSPSFSSFAE-----------------QPQSWSQLLLGEEERVL-NQTQ 60

Query: 61  MGHYQPKKLENWEDQIL------NNNHNQAPNFVDIVKQEVSNNRDQSLILQPDSTLTSS 120
           MGHYQPKKLENWE QIL      N +    PNFVDIVKQE SN                 
Sbjct: 61  MGHYQPKKLENWEHQILDHTTTTNPSPPPPPNFVDIVKQEFSN----------------- 120

Query: 121 SSHSNKFLNFSYNNSN-PD--HNINQSASQHSPE-----------SNRSAASGGACKKAR 180
              +NKFLNFS NN+N PD  HN   +A+ H PE           ++ +  SG ACKKAR
Sbjct: 121 ---TNKFLNFSSNNTNFPDLTHNQPAAAADHLPEVRFSCKSFEQLNHIAPVSGSACKKAR 180

Query: 181 IHPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVM 219
           IHPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIE +
Sbjct: 181 IHPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEAL 200

[Close]

BLAST of MS009501 vs. TAIR 10
Match: AT4G29100.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-28
Identity = 116/300 (38.67%), Postives = 154/300 (51.33%), Query Frame = 0


Query: 1   MMAGNPNWWAI-------------------------------------FP-----PSSSS 60
           M AGNPNWW +                                     FP     P++SS
Sbjct: 15  MAAGNPNWWNVSGGMRPPPPLMGHQQAPLPPHMTPNNNYLRPRMMPTPFPHFLPSPATSS 74

Query: 61  SSSSSSLFPPQFPSN-------FSDHLHPHSWS-------QLLLGDEDRV-----INNQT 120
           SSSSSS   P  P+N        S+ L P SWS        L++G+E+R+      N+  
Sbjct: 75  SSSSSS---PSLPNNPNLSSWLESNDLPPESWSLSQLLLGGLMMGEEERLEMMNHHNHHD 134

Query: 121 QMGH--YQPK-KLENWEDQILNNNHNQAPNFVDIVKQEVS-NNRDQSLILQPDS------ 180
           +  H  +Q K +LENWE+Q+L  +H QA      +KQE + NN +  +I  P+S      
Sbjct: 135 EQQHHGFQGKIRLENWEEQVL--SHQQASMVAVDIKQEGNINNNNGYVISSPNSPPNKSC 194

Query: 181 -TLTSSSSHSNKFLNFSYNNS----NPDHN-INQSASQHSP-----ESNRSAASGGACKK 219
            T T+++S ++   N + NN+    + +HN ++ S  +H+P     E N     G   KK
Sbjct: 195 VTTTTTTSLNSNDDNINNNNNMLDFSSNHNGLHLSEGRHTPPDRSSECNSLEIGGSTNKK 254

[Close]

BLAST of MS009501 vs. TAIR 10
Match: AT2G20100.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 9.6e-28
Identity = 105/268 (39.18%), Postives = 137/268 (51.12%), Query Frame = 0


Query: 3   AGNPNWW-----AIFPPS-------------------------SSSSSSSSSLFPPQFPS 62
           AGNPNWW      + PP+                         +SSSSSS S  PP    
Sbjct: 17  AGNPNWWNNVSRGLRPPTPLMSHEPPSTTAFIPSLLPNFFSSPTSSSSSSPSFPPPNSNP 76

Query: 63  NFSDHL--------HPHSWSQLLL-----GDEDRV-----INNQTQMGHYQPKKLENWED 122
           NFS  L         P S SQLLL     G+E+++      ++Q Q   YQ K+++NWE+
Sbjct: 77  NFSSWLEMSDLPLDQPWSLSQLLLGGLMMGEEEKMEMMNHHHHQNQHQSYQAKRIQNWEE 136

Query: 123 QILNNNHNQAPNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNKFLNFSYNNSNPDHN 182
           Q+L +  +        +KQE SNN    ++  P+S    S +        +  N+N D+N
Sbjct: 137 QVLRHQAS--------MKQESSNNNSYGIMSSPNSPPNKSCA--------TIINTNEDNN 196

Query: 183 INQSASQHSPESNRSAASGG--ACKKARIHPPSSSQPPLKVRKEKLVDRITALHQIVSPF 221
            N  +  +  E N S   G   A KK ++  P SSQ  LKVRKEKL  RI +LHQ+VSPF
Sbjct: 197 NNIHSGLNLSECNSSEMIGSSFANKKPKLQVP-SSQSTLKVRKEKLGGRIASLHQLVSPF 256

[Close]

BLAST of MS009501 vs. TAIR 10
Match: AT2G20100.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 9.6e-28
Identity = 105/268 (39.18%), Postives = 137/268 (51.12%), Query Frame = 0


Query: 3   AGNPNWW-----AIFPPS-------------------------SSSSSSSSSLFPPQFPS 62
           AGNPNWW      + PP+                         +SSSSSS S  PP    
Sbjct: 17  AGNPNWWNNVSRGLRPPTPLMSHEPPSTTAFIPSLLPNFFSSPTSSSSSSPSFPPPNSNP 76

Query: 63  NFSDHL--------HPHSWSQLLL-----GDEDRV-----INNQTQMGHYQPKKLENWED 122
           NFS  L         P S SQLLL     G+E+++      ++Q Q   YQ K+++NWE+
Sbjct: 77  NFSSWLEMSDLPLDQPWSLSQLLLGGLMMGEEEKMEMMNHHHHQNQHQSYQAKRIQNWEE 136

Query: 123 QILNNNHNQAPNFVDIVKQEVSNNRDQSLILQPDSTLTSSSSHSNKFLNFSYNNSNPDHN 182
           Q+L +  +        +KQE SNN    ++  P+S    S +        +  N+N D+N
Sbjct: 137 QVLRHQAS--------MKQESSNNNSYGIMSSPNSPPNKSCA--------TIINTNEDNN 196

Query: 183 INQSASQHSPESNRSAASGG--ACKKARIHPPSSSQPPLKVRKEKLVDRITALHQIVSPF 221
            N  +  +  E N S   G   A KK ++  P SSQ  LKVRKEKL  RI +LHQ+VSPF
Sbjct: 197 NNIHSGLNLSECNSSEMIGSSFANKKPKLQVP-SSQSTLKVRKEKLGGRIASLHQLVSPF 256

[Close]

BLAST of MS009501 vs. TAIR 10
Match: AT1G27660.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.4e-15
Identity = 52/100 (52.00%), Postives = 65/100 (65.00%), Query Frame = 0


Query: 128 NSNPDHNINQSASQ-HSPESNR------SAASGGACKKARIHPPSSSQPPLKVRKEKLVD 187
           +S+P H +   +++  + E  R      + A   A KK R+   SS  PP KVRKEKL D
Sbjct: 282 SSSPTHQMEMFSNEPQTSEGKRHNFLMATKAGENASKKPRVESRSSC-PPFKVRKEKLGD 341

Query: 188 RITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQIEVMPI 221
           RI AL Q+VSPFGKTDTASVL EAIGYI+FLQ QIE + +
Sbjct: 342 RIAALQQLVSPFGKTDTASVLMEAIGYIKFLQSQIETLSV 380

[Close]

BLAST of MS009501 vs. TAIR 10
Match: AT4G21340.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 7.9e-14
Identity = 41/59 (69.49%), Postives = 46/59 (77.97%), Query Frame = 0


Query: 157 KKARIHPPSSSQPPLKVRKEKLVDRITALHQIVSPFGKTDTASVLSEAIGYIRFLQGQI 216
           K+ R+  PS   P  KVRKEKL DRITAL Q+VSPFGKTDTASVL +AI YI+FLQ QI
Sbjct: 176 KRPRLETPSHF-PSFKVRKEKLGDRITALQQLVSPFGKTDTASVLHDAIDYIKFLQEQI 233

The following BLAST results are available for this feature:

BLAST of MS009501 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146101.1	2.2e-111	96.33	transcription factor bHLH68-like [Momordica charantia]	[more]
XP_038897153.1	3.2e-65	66.81	LOW QUALITY PROTEIN: transcription factor bHLH133-like [Benincasa hispida]	[more]
TYK04014.1	4.3e-62	64.68	transcription factor bHLH68-like [Cucumis melo var. makuwa]	[more]
XP_004141839.1	6.2e-61	63.71	transcription factor bHLH68 [Cucumis sativus] >KGN45389.1 hypothetical protein C...	[more]
XP_008465633.2	8.9e-60	64.07	PREDICTED: transcription factor bHLH68-like, partial [Cucumis melo]	[more]

BLAST of MS009501 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S3D1	1.6e-27	38.67	Transcription factor bHLH68 OS=Arabidopsis thaliana OX=3702 GN=BHLH68 PE=1 SV=2	[more]
Q7XHI5	1.4e-26	39.18	Transcription factor bHLH133 OS=Arabidopsis thaliana OX=3702 GN=BHLH133 PE=1 SV=...	[more]
Q9SFZ3	2.0e-14	52.00	Transcription factor bHLH110 OS=Arabidopsis thaliana OX=3702 GN=BHLH110 PE=2 SV=...	[more]
Q8VZ22	1.1e-12	69.49	Transcription factor bHLH103 OS=Arabidopsis thaliana OX=3702 GN=BHLH103 PE=1 SV=...	[more]
Q9LT67	8.0e-11	39.23	Transcription factor bHLH113 OS=Arabidopsis thaliana OX=3702 GN=BHLH113 PE=2 SV=...	[more]

BLAST of MS009501 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CYC1	1.1e-111	96.33	transcription factor bHLH68-like OS=Momordica charantia OX=3673 GN=LOC111015393 ...	[more]
A0A5D3BYE2	2.1e-62	64.68	Transcription factor bHLH68-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A0A0K957	3.0e-61	63.71	BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447040 PE=4 S...	[more]
A0A1S3CQT4	4.3e-60	64.07	transcription factor bHLH68-like OS=Cucumis melo OX=3656 GN=LOC103503272 PE=4 SV...	[more]
A0A6J1HGP4	3.2e-55	59.24	transcription factor bHLH68-like OS=Cucurbita moschata OX=3662 GN=LOC111464167 P...	[more]

BLAST of MS009501 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29100.1	1.1e-28	38.67	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G20100.1	9.6e-28	39.18	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G20100.2	9.6e-28	39.18	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G27660.1	1.4e-15	52.00	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT4G21340.1	7.9e-14	69.49	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 172..219 e-value: 1.4E-5 score: 27.1
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 172..218
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 122..150
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 122..173
None	No IPR available	PANTHER	PTHR16223	TRANSCRIPTION FACTOR BHLH83-RELATED	coord: 8..219
None	No IPR available	PANTHER	PTHR16223:SF53	BHLH TRANSCRIPTION FACTOR-LIKE PROTEIN	coord: 8..219
None	No IPR available	CDD	cd11393	bHLH_AtbHLH_like	coord: 172..218 e-value: 1.23415E-20 score: 79.5327
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 162..211 score: 10.867585

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009501.1	MS009501.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046983	protein dimerization activity