MS009499 (gene) Bitter gourd (TR) v1

Overview


Name	MS009499
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB
Location	scaffold813: 1760270 .. 1761593 (-)
RNA-Seq Expression	MS009499
Synteny	MS009499

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAATCAGTGGAACCACACTGCTCTTCTCGTCATTGACATGCAGGTTTTCCTATTTTCTACTAATTTTTCTTTCCCTTCCTTCCTGCTCACGATCAATTTCTTCTTATTTCAGAGAGATTTCTTTGACGAACGGTCTGTCATAGCTATACCAGGAGCCTATGCTATAGTTCCGAGCGTGGTAGATGCAGTCGAAATTGCTAGGAAGCGGAGAATGCTCATTGTTTGGGTCAGTTTCTTCCTCTATTTTCGGTTCTTTTCTCCTTGGAATTATACCCGCTGTCAAAATCCCCCCGAAGAGTAGTCTCTGATCGTTTTTACTTCACAGAATGAATTAGGTTTTCTAAATTGTGAAACATTAGGGTTACAGATTAGTCTGATATGTATAACAAAATGAACCCAGGTGCCCTGTTTTAAGTGTTTGGTAGCTGAGAAAATGTCTGAATGAAAACTATTGGAAAGAAAGATAACCTCTTTTGTGTTTCTGTTCCTTTGTTTATCTGATTGCTGCGCATGTTAATTATGCTTATTTCAAGATGCAGCATCCATAAATTTATTTCATTGACTTTGTTAGTTAACTCTTCCAACTCTTGAATTCAACTGATTGCTATTCAATTAGCCTAATTGAAGAATAATGGGGGTGATTTTTTCTTTATCATAGGTTGTACGGGAGCACGACCCTCAAGGACGAGATGTCGAACGCTTTCGTCGCCATTTCTATGGCAGTGGGAAGCAGAATCCGGTTGCGAAGGGAAGCAGAGGGGCAGAGTTAGTAGATGGCCTTGAAATCAAAGAAGGAGAATACAAGTTGGTTAAAACCAGGTTCAGTGCCTTCTTTAACACTAACTTGCATTCACTTCTGCAGAGGGAGGGTATTAACAATCTGGTTGTCGCGGGTAAGTTTCGACATATACATATCTGACGTTACCATATTTCGAATATGAATTTGTAGAGTGGAATGTTAATGAATGGTTGATGCATATTCTAGGTGTTCAAACCCCGAACTGTATTAGGCAGACTGTTTTTGATGCTGTAGCGTTGGATTACCATTCTATCACCCTTCTTCATGATGCAACAGCAGCCGCTACGCCTAAAACTCATCATGGTATGTCATCTAAATCCTTTCCCGTTCTAAGAACAATACATAGATGGCTATGATTTTGCTACTGGTGAAGAACATTGCTGACAAAGTTTGGGAGATGAAATCAAATGAATGATGAGGGAATTTCTGAATTTTTTTTTCTGTATCAGACAATATTGCTGACATGGAAAATGTGGGAGTTGTGGCCAAACGAGTCGACCAATGGGGCGAGTCTGATGAT

mRNA sequence

ATGGCGAATCAGTGGAACCACACTGCTCTTCTCGTCATTGACATGCAGGTTTTCCTATTTTCTACTAATTTTTCTTTCCCTTCCTTCCTGCTCACGATCAATTTCTTCTTATTTCAGAGAGATTTCTTTGACGAACGGTCTGTCATAGCTATACCAGGAGCCTATGCTATAGTTCCGAGCGTGGTAGATGCAGTCGAAATTGCTAGGAAGCGGAGAATGCTCATTGTTTGGGTTGTACGGGAGCACGACCCTCAAGGACGAGATGTCGAACGCTTTCGTCGCCATTTCTATGGCAGTGGGAAGCAGAATCCGGTTGCGAAGGGAAGCAGAGGGGCAGAGTTAGTAGATGGCCTTGAAATCAAAGAAGGAGAATACAAGTTGGTTAAAACCAGGTTCAGTGCCTTCTTTAACACTAACTTGCATTCACTTCTGCAGAGGGAGGGTATTAACAATCTGGTTGTCGCGGGTGTTCAAACCCCGAACTGTATTAGGCAGACTGTTTTTGATGCTGTAGCGTTGGATTACCATTCTATCACCCTTCTTCATGATGCAACAGCAGCCGCTACGCCTAAAACTCATCATGACAATATTGCTGACATGGAAAATGTGGGAGTTGTGGCCAAACGAGTCGACCAATGGGGCGAGTCTGATGAT

Coding sequence (CDS)

ATGGCGAATCAGTGGAACCACACTGCTCTTCTCGTCATTGACATGCAGGTTTTCCTATTTTCTACTAATTTTTCTTTCCCTTCCTTCCTGCTCACGATCAATTTCTTCTTATTTCAGAGAGATTTCTTTGACGAACGGTCTGTCATAGCTATACCAGGAGCCTATGCTATAGTTCCGAGCGTGGTAGATGCAGTCGAAATTGCTAGGAAGCGGAGAATGCTCATTGTTTGGGTTGTACGGGAGCACGACCCTCAAGGACGAGATGTCGAACGCTTTCGTCGCCATTTCTATGGCAGTGGGAAGCAGAATCCGGTTGCGAAGGGAAGCAGAGGGGCAGAGTTAGTAGATGGCCTTGAAATCAAAGAAGGAGAATACAAGTTGGTTAAAACCAGGTTCAGTGCCTTCTTTAACACTAACTTGCATTCACTTCTGCAGAGGGAGGGTATTAACAATCTGGTTGTCGCGGGTGTTCAAACCCCGAACTGTATTAGGCAGACTGTTTTTGATGCTGTAGCGTTGGATTACCATTCTATCACCCTTCTTCATGATGCAACAGCAGCCGCTACGCCTAAAACTCATCATGACAATATTGCTGACATGGAAAATGTGGGAGTTGTGGCCAAACGAGTCGACCAATGGGGCGAGTCTGATGAT

Protein sequence

MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPSVVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEIKEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITLLHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESDD

Homology

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BLAST of MS009499 vs. NCBI nr
Match: XP_022154038.1 (probable inactive nicotinamidase At3g16190 [Momordica charantia])

HSP 1 Score: 378.6 bits (971), Expect = 3.5e-101
Identity = 194/218 (88.99%), Postives = 195/218 (89.45%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MANQWNHTALLVIDM                       QRDFFDERSVIAIPGAYAIVPS
Sbjct: 1   MANQWNHTALLVIDM-----------------------QRDFFDERSVIAIPGAYAIVPS 60

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           VVDAVEIARKRRMLIVWVVREHDP+GRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI
Sbjct: 61  VVDAVEIARKRRMLIVWVVREHDPEGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120

Query: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180
           KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL
Sbjct: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180

Query: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESDD 219
           LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESDD
Sbjct: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESDD 195

[Close]

BLAST of MS009499 vs. NCBI nr
Match: TYK04015.1 (putative inactive nicotinamidase [Cucumis melo var. makuwa])

HSP 1 Score: 319.3 bits (817), Expect = 2.5e-83
Identity = 165/222 (74.32%), Postives = 181/222 (81.53%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLF-----STNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAY 60
           MA+QW  TALLVIDMQV  F     S  FS P+ +L  NFF  QRDF DE SV A+PGA 
Sbjct: 1   MADQWKRTALLVIDMQVXPFYFLPNSIPFSTPTIIL-FNFFQLQRDFIDEHSVFAVPGAS 60

Query: 61  AIVPSVVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELV 120
            I+PSV  AVEIAR R + I+WVVREHD +GRDVERFRRH+YG+GK NP+ KGS+GAELV
Sbjct: 61  TIIPSVSTAVEIARNRGLFIIWVVREHDGEGRDVERFRRHYYGNGKPNPLVKGSKGAELV 120

Query: 121 DGLEIKEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDY 180
            GLEIKEGEYKLVKTRFSAFFNTNL SLLQ  GI +LVV GVQTPNCIRQTV+DAVALDY
Sbjct: 121 QGLEIKEGEYKLVKTRFSAFFNTNLRSLLQGAGITDLVVCGVQTPNCIRQTVYDAVALDY 180

Query: 181 HSITLLHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESD 218
           HSITLL+DATAAATPK HHDNI DM NVGV  KRVD+WGESD
Sbjct: 181 HSITLLYDATAAATPKVHHDNITDMVNVGVEVKRVDEWGESD 221

[Close]

BLAST of MS009499 vs. NCBI nr
Match: XP_023000390.1 (probable inactive nicotinamidase At3g16190 [Cucurbita maxima])

HSP 1 Score: 313.2 bits (801), Expect = 1.8e-81
Identity = 155/216 (71.76%), Postives = 174/216 (80.56%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MAN+W+HTALL+ID                       FQRDFFDERSV A+PGAYAI+PS
Sbjct: 3   MANEWSHTALLIID-----------------------FQRDFFDERSVFAVPGAYAILPS 62

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           V DA+E ARKR M +VWVVREHDP+GRDVERFRRH YGSGKQNPV+KGS GAEL++G EI
Sbjct: 63  VYDALECARKRGMFVVWVVREHDPEGRDVERFRRHLYGSGKQNPVSKGSIGAELIEGFEI 122

Query: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180
           KEGEYKLVKTRFSAFFNTNLHSLLQ  GI +LV+ GVQTPNCIRQTVFDA++LDYHSIT+
Sbjct: 123 KEGEYKLVKTRFSAFFNTNLHSLLQASGITDLVICGVQTPNCIRQTVFDAISLDYHSITV 182

Query: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGES 217
           L+DATAAA+ + HHDNI DMENVGVV  RVDQW  S
Sbjct: 183 LYDATAAASVQIHHDNITDMENVGVVVVRVDQWDGS 195

[Close]

BLAST of MS009499 vs. NCBI nr
Match: KAG6593471.1 (putative inactive nicotinamidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025814.1 putative inactive nicotinamidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 309.7 bits (792), Expect = 2.0e-80
Identity = 154/216 (71.30%), Postives = 173/216 (80.09%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MAN+W+ TALL+ID                       FQRDFFDERSV A+PGAYAI+PS
Sbjct: 3   MANEWSRTALLIID-----------------------FQRDFFDERSVFAVPGAYAILPS 62

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           V DA+E ARKR M +VWVVREHDP+GRDVERFRRH YGSGKQNPV+KGS GAEL++G EI
Sbjct: 63  VYDALECARKRGMFVVWVVREHDPEGRDVERFRRHLYGSGKQNPVSKGSIGAELIEGFEI 122

Query: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180
           KEGEYKLVKTRFSAFFNTNLHSLLQ  GI +LV+ GVQTPNCIRQTVFDA++LDYHSIT+
Sbjct: 123 KEGEYKLVKTRFSAFFNTNLHSLLQATGITDLVICGVQTPNCIRQTVFDAISLDYHSITV 182

Query: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGES 217
           L+DATAAA+ + HHDNI DMENVGVV  RVDQW  S
Sbjct: 183 LYDATAAASVQIHHDNITDMENVGVVVVRVDQWDGS 195

[Close]

BLAST of MS009499 vs. NCBI nr
Match: XP_022964605.1 (probable inactive nicotinamidase At3g16190 [Cucurbita moschata])

HSP 1 Score: 309.7 bits (792), Expect = 2.0e-80
Identity = 154/216 (71.30%), Postives = 173/216 (80.09%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MAN+W+ TALL+ID                       FQRDFFDERSV A+PGAYAI+PS
Sbjct: 3   MANEWSRTALLIID-----------------------FQRDFFDERSVFAVPGAYAILPS 62

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           V DA+E ARKR M +VWVVREHDP+GRDVERFRRH YGSGKQNPV+KGS GAEL++G EI
Sbjct: 63  VYDALECARKRGMFVVWVVREHDPEGRDVERFRRHLYGSGKQNPVSKGSIGAELIEGFEI 122

Query: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180
           KEGEYKLVKTRFSAFFNTNLHSLLQ  GI +LV+ GVQTPNCIRQTVFDA++LDYHSIT+
Sbjct: 123 KEGEYKLVKTRFSAFFNTNLHSLLQATGITDLVICGVQTPNCIRQTVFDAISLDYHSITV 182

Query: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGES 217
           L+DATAAA+ + HHDNI DMENVGVV  RVDQW  S
Sbjct: 183 LYDATAAASVQIHHDNITDMENVGVVVVRVDQWDGS 195

[Close]

BLAST of MS009499 vs. ExPASy Swiss-Prot
Match: Q93Z51 (Probable inactive nicotinamidase At3g16190 OS=Arabidopsis thaliana OX=3702 GN=At3g16190 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.4e-60
Identity = 122/216 (56.48%), Postives = 151/216 (69.91%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MA +W +TALLVIDM                       Q DF +E +V  + G  +IVP+
Sbjct: 1   MAERWRNTALLVIDM-----------------------QNDFIEEGAVTQVKGGKSIVPN 60

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           V+  VE+AR+R +L++WVVREHD QGRDVE FRRH Y S K  PV KG+ GAELVDGL I
Sbjct: 61  VIRVVELARQRGILVIWVVREHDRQGRDVELFRRHNYSSEKVGPVIKGTVGAELVDGLMI 120

Query: 121 -KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSIT 180
            +E +YK+VKTRFSAFF+TNLHS LQ  G+  LV+AGVQTPNCIRQTVFDAVALDY ++T
Sbjct: 121 NEEDDYKIVKTRFSAFFSTNLHSFLQTSGVTKLVIAGVQTPNCIRQTVFDAVALDYPNVT 180

Query: 181 LLHDATAAATPKTHHDNIADMENVGVVAKRVDQWGE 216
           ++ DATAAATP+ H  NI DM+N+GV    + +W E
Sbjct: 181 VITDATAAATPEIHTANILDMKNIGVKTPTLHEWSE 193

[Close]

BLAST of MS009499 vs. ExPASy Swiss-Prot
Match: C4ZQD9 (Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain K12 / MC4100 / BW2952) OX=595496 GN=rutB PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.4e-12
Identity = 56/174 (32.18%), Postives = 80/174 (45.98%), Query Frame = 0


Query: 44  DERSVIAIPGAY------------AIVPSVVDAVEIARKRRMLIVWVVREHDPQ------ 103
           D ++  A PG Y             ++ ++  AV  AR   MLI+W     D Q      
Sbjct: 24  DMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGG 83

Query: 104 --------GRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEIKEGEYKLVKTRFSAFFN 163
                      ++  R+     GK   +AKGS   +LVD L  + G+  L K R+S FFN
Sbjct: 84  PGSPNFHKSNALKTMRKQPQLQGKL--LAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFN 143

Query: 164 TNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITLLHDATAAATPK 192
           T L S+L+  GI +LV  G+ T  C+  T+ D   L+Y  + +L DAT  A PK
Sbjct: 144 TPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGV-VLEDATHQAGPK 194

[Close]

BLAST of MS009499 vs. ExPASy Swiss-Prot
Match: C9QZ65 (Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain ATCC 33849 / DSM 4235 / NCIMB 12045 / K12 / DH1) OX=536056 GN=rutB PE=3 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 2.4e-12
Identity = 56/174 (32.18%), Postives = 80/174 (45.98%), Query Frame = 0


Query: 44  DERSVIAIPGAY------------AIVPSVVDAVEIARKRRMLIVWVVREHDPQ------ 103
           D ++  A PG Y             ++ ++  AV  AR   MLI+W     D Q      
Sbjct: 24  DMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGG 83

Query: 104 --------GRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEIKEGEYKLVKTRFSAFFN 163
                      ++  R+     GK   +AKGS   +LVD L  + G+  L K R+S FFN
Sbjct: 84  PGSPNFHKSNALKTMRKQPQLQGKL--LAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFN 143

Query: 164 TNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITLLHDATAAATPK 192
           T L S+L+  GI +LV  G+ T  C+  T+ D   L+Y  + +L DAT  A PK
Sbjct: 144 TPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGV-VLEDATHQAGPK 194

[Close]

BLAST of MS009499 vs. ExPASy Swiss-Prot
Match: B1X9D2 (Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain K12 / DH10B) OX=316385 GN=rutB PE=3 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.4e-12
Identity = 56/174 (32.18%), Postives = 80/174 (45.98%), Query Frame = 0


Query: 44  DERSVIAIPGAY------------AIVPSVVDAVEIARKRRMLIVWVVREHDPQ------ 103
           D ++  A PG Y             ++ ++  AV  AR   MLI+W     D Q      
Sbjct: 24  DMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGG 83

Query: 104 --------GRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEIKEGEYKLVKTRFSAFFN 163
                      ++  R+     GK   +AKGS   +LVD L  + G+  L K R+S FFN
Sbjct: 84  PGSPNFHKSNALKTMRKQPQLQGKL--LAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFN 143

Query: 164 TNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITLLHDATAAATPK 192
           T L S+L+  GI +LV  G+ T  C+  T+ D   L+Y  + +L DAT  A PK
Sbjct: 144 TPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGV-VLEDATHQAGPK 194

[Close]

BLAST of MS009499 vs. ExPASy Swiss-Prot
Match: P75897 (Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain K12) OX=83333 GN=rutB PE=1 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 2.4e-12
Identity = 56/174 (32.18%), Postives = 80/174 (45.98%), Query Frame = 0


Query: 44  DERSVIAIPGAY------------AIVPSVVDAVEIARKRRMLIVWVVREHDPQ------ 103
           D ++  A PG Y             ++ ++  AV  AR   MLI+W     D Q      
Sbjct: 24  DMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGG 83

Query: 104 --------GRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEIKEGEYKLVKTRFSAFFN 163
                      ++  R+     GK   +AKGS   +LVD L  + G+  L K R+S FFN
Sbjct: 84  PGSPNFHKSNALKTMRKQPQLQGKL--LAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFN 143

Query: 164 TNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITLLHDATAAATPK 192
           T L S+L+  GI +LV  G+ T  C+  T+ D   L+Y  + +L DAT  A PK
Sbjct: 144 TPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYFGV-VLEDATHQAGPK 194

[Close]

BLAST of MS009499 vs. ExPASy TrEMBL
Match: A0A6J1DJ62 (probable inactive nicotinamidase At3g16190 OS=Momordica charantia OX=3673 GN=LOC111021389 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.7e-101
Identity = 194/218 (88.99%), Postives = 195/218 (89.45%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MANQWNHTALLVIDM                       QRDFFDERSVIAIPGAYAIVPS
Sbjct: 1   MANQWNHTALLVIDM-----------------------QRDFFDERSVIAIPGAYAIVPS 60

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           VVDAVEIARKRRMLIVWVVREHDP+GRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI
Sbjct: 61  VVDAVEIARKRRMLIVWVVREHDPEGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120

Query: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180
           KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL
Sbjct: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180

Query: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESDD 219
           LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESDD
Sbjct: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESDD 195

[Close]

BLAST of MS009499 vs. ExPASy TrEMBL
Match: A0A5D3BXX3 (Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002130 PE=3 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 1.2e-83
Identity = 165/222 (74.32%), Postives = 181/222 (81.53%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLF-----STNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAY 60
           MA+QW  TALLVIDMQV  F     S  FS P+ +L  NFF  QRDF DE SV A+PGA 
Sbjct: 1   MADQWKRTALLVIDMQVXPFYFLPNSIPFSTPTIIL-FNFFQLQRDFIDEHSVFAVPGAS 60

Query: 61  AIVPSVVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELV 120
            I+PSV  AVEIAR R + I+WVVREHD +GRDVERFRRH+YG+GK NP+ KGS+GAELV
Sbjct: 61  TIIPSVSTAVEIARNRGLFIIWVVREHDGEGRDVERFRRHYYGNGKPNPLVKGSKGAELV 120

Query: 121 DGLEIKEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDY 180
            GLEIKEGEYKLVKTRFSAFFNTNL SLLQ  GI +LVV GVQTPNCIRQTV+DAVALDY
Sbjct: 121 QGLEIKEGEYKLVKTRFSAFFNTNLRSLLQGAGITDLVVCGVQTPNCIRQTVYDAVALDY 180

Query: 181 HSITLLHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESD 218
           HSITLL+DATAAATPK HHDNI DM NVGV  KRVD+WGESD
Sbjct: 181 HSITLLYDATAAATPKVHHDNITDMVNVGVEVKRVDEWGESD 221

[Close]

BLAST of MS009499 vs. ExPASy TrEMBL
Match: A0A6J1KI74 (probable inactive nicotinamidase At3g16190 OS=Cucurbita maxima OX=3661 GN=LOC111494647 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 8.8e-82
Identity = 155/216 (71.76%), Postives = 174/216 (80.56%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MAN+W+HTALL+ID                       FQRDFFDERSV A+PGAYAI+PS
Sbjct: 3   MANEWSHTALLIID-----------------------FQRDFFDERSVFAVPGAYAILPS 62

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           V DA+E ARKR M +VWVVREHDP+GRDVERFRRH YGSGKQNPV+KGS GAEL++G EI
Sbjct: 63  VYDALECARKRGMFVVWVVREHDPEGRDVERFRRHLYGSGKQNPVSKGSIGAELIEGFEI 122

Query: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180
           KEGEYKLVKTRFSAFFNTNLHSLLQ  GI +LV+ GVQTPNCIRQTVFDA++LDYHSIT+
Sbjct: 123 KEGEYKLVKTRFSAFFNTNLHSLLQASGITDLVICGVQTPNCIRQTVFDAISLDYHSITV 182

Query: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGES 217
           L+DATAAA+ + HHDNI DMENVGVV  RVDQW  S
Sbjct: 183 LYDATAAASVQIHHDNITDMENVGVVVVRVDQWDGS 195

[Close]

BLAST of MS009499 vs. ExPASy TrEMBL
Match: A0A6J1HLA4 (probable inactive nicotinamidase At3g16190 OS=Cucurbita moschata OX=3662 GN=LOC111464568 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 9.8e-81
Identity = 154/216 (71.30%), Postives = 173/216 (80.09%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MAN+W+ TALL+ID                       FQRDFFDERSV A+PGAYAI+PS
Sbjct: 3   MANEWSRTALLIID-----------------------FQRDFFDERSVFAVPGAYAILPS 62

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           V DA+E ARKR M +VWVVREHDP+GRDVERFRRH YGSGKQNPV+KGS GAEL++G EI
Sbjct: 63  VYDALECARKRGMFVVWVVREHDPEGRDVERFRRHLYGSGKQNPVSKGSIGAELIEGFEI 122

Query: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180
           KEGEYKLVKTRFSAFFNTNLHSLLQ  GI +LV+ GVQTPNCIRQTVFDA++LDYHSIT+
Sbjct: 123 KEGEYKLVKTRFSAFFNTNLHSLLQATGITDLVICGVQTPNCIRQTVFDAISLDYHSITV 182

Query: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGES 217
           L+DATAAA+ + HHDNI DMENVGVV  RVDQW  S
Sbjct: 183 LYDATAAASVQIHHDNITDMENVGVVVVRVDQWDGS 195

[Close]

BLAST of MS009499 vs. ExPASy TrEMBL
Match: A0A6J1GPU8 (probable inactive nicotinamidase At3g16190 OS=Cucurbita moschata OX=3662 GN=LOC111456377 PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 3.1e-79
Identity = 155/217 (71.43%), Postives = 172/217 (79.26%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MA+QWNHTALLVIDM                       Q+DF DE S  AIPGA AI+PS
Sbjct: 1   MADQWNHTALLVIDM-----------------------QKDFIDEGSPFAIPGARAILPS 60

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           V  AVEIARKR + ++WVVREHDP+GRDVERFRRH+YG+GK  P AKGSRGAELV+GLE+
Sbjct: 61  VYRAVEIARKRGVFVIWVVREHDPEGRDVERFRRHYYGNGKLKPAAKGSRGAELVEGLEM 120

Query: 121 KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSITL 180
           KEGEYKLVKTRFSAFF+TNLHSLLQ  GI +LVVAGVQTPNCIRQTVFD V+LDYHSITL
Sbjct: 121 KEGEYKLVKTRFSAFFDTNLHSLLQGMGITHLVVAGVQTPNCIRQTVFDGVSLDYHSITL 180

Query: 181 LHDATAAATPKTHHDNIADMENVGVVAKRVDQWGESD 218
           L DATAAATPK H DNI DM+NVGVVA +VD+WG SD
Sbjct: 181 LSDATAAATPKIHQDNITDMKNVGVVASKVDEWGHSD 194

[Close]

BLAST of MS009499 vs. TAIR 10
Match: AT3G16190.1 (Isochorismatase family protein )

HSP 1 Score: 234.2 bits (596), Expect = 1.0e-61
Identity = 122/216 (56.48%), Postives = 151/216 (69.91%), Query Frame = 0


Query: 1   MANQWNHTALLVIDMQVFLFSTNFSFPSFLLTINFFLFQRDFFDERSVIAIPGAYAIVPS 60
           MA +W +TALLVIDM                       Q DF +E +V  + G  +IVP+
Sbjct: 1   MAERWRNTALLVIDM-----------------------QNDFIEEGAVTQVKGGKSIVPN 60

Query: 61  VVDAVEIARKRRMLIVWVVREHDPQGRDVERFRRHFYGSGKQNPVAKGSRGAELVDGLEI 120
           V+  VE+AR+R +L++WVVREHD QGRDVE FRRH Y S K  PV KG+ GAELVDGL I
Sbjct: 61  VIRVVELARQRGILVIWVVREHDRQGRDVELFRRHNYSSEKVGPVIKGTVGAELVDGLMI 120

Query: 121 -KEGEYKLVKTRFSAFFNTNLHSLLQREGINNLVVAGVQTPNCIRQTVFDAVALDYHSIT 180
            +E +YK+VKTRFSAFF+TNLHS LQ  G+  LV+AGVQTPNCIRQTVFDAVALDY ++T
Sbjct: 121 NEEDDYKIVKTRFSAFFSTNLHSFLQTSGVTKLVIAGVQTPNCIRQTVFDAVALDYPNVT 180

Query: 181 LLHDATAAATPKTHHDNIADMENVGVVAKRVDQWGE 216
           ++ DATAAATP+ H  NI DM+N+GV    + +W E
Sbjct: 181 VITDATAAATPEIHTANILDMKNIGVKTPTLHEWSE 193

The following BLAST results are available for this feature:

BLAST of MS009499 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154038.1	3.5e-101	88.99	probable inactive nicotinamidase At3g16190 [Momordica charantia]	[more]
TYK04015.1	2.5e-83	74.32	putative inactive nicotinamidase [Cucumis melo var. makuwa]	[more]
XP_023000390.1	1.8e-81	71.76	probable inactive nicotinamidase At3g16190 [Cucurbita maxima]	[more]
KAG6593471.1	2.0e-80	71.30	putative inactive nicotinamidase, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022964605.1	2.0e-80	71.30	probable inactive nicotinamidase At3g16190 [Cucurbita moschata]	[more]

BLAST of MS009499 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z51	1.4e-60	56.48	Probable inactive nicotinamidase At3g16190 OS=Arabidopsis thaliana OX=3702 GN=At...	[more]
C4ZQD9	2.4e-12	32.18	Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain K12 / MC4100 / BW...	[more]
C9QZ65	2.4e-12	32.18	Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain ATCC 33849 / DSM ...	[more]
B1X9D2	2.4e-12	32.18	Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain K12 / DH10B) OX=3...	[more]
P75897	2.4e-12	32.18	Ureidoacrylate amidohydrolase RutB OS=Escherichia coli (strain K12) OX=83333 GN=...	[more]

BLAST of MS009499 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DJ62	1.7e-101	88.99	probable inactive nicotinamidase At3g16190 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A5D3BXX3	1.2e-83	74.32	Putative inactive nicotinamidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1KI74	8.8e-82	71.76	probable inactive nicotinamidase At3g16190 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1HLA4	9.8e-81	71.30	probable inactive nicotinamidase At3g16190 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1GPU8	3.1e-79	71.43	probable inactive nicotinamidase At3g16190 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of MS009499 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G16190.1	1.0e-61	56.48	Isochorismatase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000868	Isochorismatase-like	PFAM	PF00857	Isochorismatase	coord: 8..205 e-value: 6.5E-35 score: 120.9
IPR036380	Isochorismatase-like superfamily	GENE3D	3.40.50.850		coord: 38..216 e-value: 1.2E-44 score: 154.6
IPR036380	Isochorismatase-like superfamily	SUPERFAMILY	52499	Isochorismatase-like hydrolases	coord: 38..213
None	No IPR available	PANTHER	PTHR47044	OS02G0276400 PROTEIN	coord: 1..215
None	No IPR available	CDD	cd00431	cysteine_hydrolases	coord: 9..201 e-value: 5.6466E-39 score: 130.081

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009499.1	MS009499.1	mRNA