MS009488 (gene) Bitter gourd (TR) v1

Overview
NameMS009488
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionhydroxyphenylpyruvate reductase-like
Locationscaffold813: 1678189 .. 1679537 (-)
RNA-Seq ExpressionMS009488
SyntenyMS009488
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGATAGGAGTCGCAATGACGACCCCCATGTTCGATTACCTTGAACAACAGCTTGAACAGCGTTTCAAGCTCTTCAAACTCTGGAACCATCCCCTGAACTCCGATTACGTTCGAGAAAACGCGCATTCCATCAGAGCAGTAGTCGGCGGCACCAAGTCCGGCGCCGACGCTAATTTGATACACTCCTTGCCTGCGTTGGAAATCGTTGCGAGCTTCAGCGTTGGTCTGGACAAGATTGATTTGCCAAAATGCCAGGAGAAGGGGATAAGGGTTACCAATACGCCCGACGTATTGACTGACGACGTCGCAGATGCCGCGATTGGACTTGCCATGGCGGTTTCGAGGAGGATTTGTGAGTCTGATCGGTTTGTCCGAAGTGGATCGTGGAAGGAAGGCGGTTTCCGATTGGCGACGAAGGTATGCTGCTTTCATTGCATTACTTTGTGCATTCCGCGCTATCTCTTACCTTAATTTGACTATGGTGAATCCTTGATGATATTGCTTGTTACGATTAAGCTCGACTGCGGGGGGAGGAACCTTAGATACTAGATTGCAGAATGTTTCTGTTAGATTCCATAGATATTAGATTGCAGAATGTTTCTGTTAGATTCCAGATATTTTCATTCATTCGATCTTGATGAACCGCCAAGATTACACTCTATCGCGATCCGACTTCATATCATTTTTCTCATCCTACTCTCTAAACCTTGGGACCAGAAGTATAGTCGAGTTTGAAGATTATAGAATTTTCCCTTACTATGAACTATGGCGAATCTATATTTCTTGGTTTGGTTGATTGACGCAGTAGTTGTTTCTTTCAGTTCAGTGGTAAATCAGTTGGAATTTTGGGGTTAGGAAGGATTGGTTCAGCCATTGCCAAGAGAGTTGAAGCATTTGGTTGCCCCATCAGTTATTGCTCTAGGAATGAAAAGCATCACAGTGGTTACAATTACAAATACTTTTCCAATGCCATAGACTTGGCTGCAAATTCCCAGATTTTGTTTGTGGCATGCACTTTGACTGAAGAAACTAAGCACATTGTCAATCGAGAAGTAATTGATGCACTGGGGCCAAATGGAATTGTTATCAACGTTGGACGAGGTGCTCATGTCAATGAATCTGAGCTTGTCTCAGCATTACTTGAAGGTCGTTTGGCAGGGGCAGGACTTGATGTATTTGAAAACGAGCCCCATGTACCTGAACAGTTGTTTGAGCTTGAAAACGTAGTTCTTCTGCCTCACGTGGGAACTGATACCATGGAGACCAGCACCGCAATGGCAGACCTTGTAATTAGAAACTTGGAGGCACATTTCAGGAATGAGCCTCTGATTACTCCTGTGATT

mRNA sequence

ATGGAGAAGATAGGAGTCGCAATGACGACCCCCATGTTCGATTACCTTGAACAACAGCTTGAACAGCGTTTCAAGCTCTTCAAACTCTGGAACCATCCCCTGAACTCCGATTACGTTCGAGAAAACGCGCATTCCATCAGAGCAGTAGTCGGCGGCACCAAGTCCGGCGCCGACGCTAATTTGATACACTCCTTGCCTGCGTTGGAAATCGTTGCGAGCTTCAGCGTTGGTCTGGACAAGATTGATTTGCCAAAATGCCAGGAGAAGGGGATAAGGGTTACCAATACGCCCGACGTATTGACTGACGACGTCGCAGATGCCGCGATTGGACTTGCCATGGCGGTTTCGAGGAGGATTTGTGAGTCTGATCGGTTTGTCCGAAGTGGATCGTGGAAGGAAGGCGGTTTCCGATTGGCGACGAAGTTCAGTGGTAAATCAGTTGGAATTTTGGGGTTAGGAAGGATTGGTTCAGCCATTGCCAAGAGAGTTGAAGCATTTGGTTGCCCCATCAGTTATTGCTCTAGGAATGAAAAGCATCACAGTGGTTACAATTACAAATACTTTTCCAATGCCATAGACTTGGCTGCAAATTCCCAGATTTTGTTTGTGGCATGCACTTTGACTGAAGAAACTAAGCACATTGTCAATCGAGAAGTAATTGATGCACTGGGGCCAAATGGAATTGTTATCAACGTTGGACGAGGTGCTCATGTCAATGAATCTGAGCTTGTCTCAGCATTACTTGAAGGTCGTTTGGCAGGGGCAGGACTTGATGTATTTGAAAACGAGCCCCATGTACCTGAACAGTTGTTTGAGCTTGAAAACGTAGTTCTTCTGCCTCACGTGGGAACTGATACCATGGAGACCAGCACCGCAATGGCAGACCTTGTAATTAGAAACTTGGAGGCACATTTCAGGAATGAGCCTCTGATTACTCCTGTGATT

Coding sequence (CDS)

ATGGAGAAGATAGGAGTCGCAATGACGACCCCCATGTTCGATTACCTTGAACAACAGCTTGAACAGCGTTTCAAGCTCTTCAAACTCTGGAACCATCCCCTGAACTCCGATTACGTTCGAGAAAACGCGCATTCCATCAGAGCAGTAGTCGGCGGCACCAAGTCCGGCGCCGACGCTAATTTGATACACTCCTTGCCTGCGTTGGAAATCGTTGCGAGCTTCAGCGTTGGTCTGGACAAGATTGATTTGCCAAAATGCCAGGAGAAGGGGATAAGGGTTACCAATACGCCCGACGTATTGACTGACGACGTCGCAGATGCCGCGATTGGACTTGCCATGGCGGTTTCGAGGAGGATTTGTGAGTCTGATCGGTTTGTCCGAAGTGGATCGTGGAAGGAAGGCGGTTTCCGATTGGCGACGAAGTTCAGTGGTAAATCAGTTGGAATTTTGGGGTTAGGAAGGATTGGTTCAGCCATTGCCAAGAGAGTTGAAGCATTTGGTTGCCCCATCAGTTATTGCTCTAGGAATGAAAAGCATCACAGTGGTTACAATTACAAATACTTTTCCAATGCCATAGACTTGGCTGCAAATTCCCAGATTTTGTTTGTGGCATGCACTTTGACTGAAGAAACTAAGCACATTGTCAATCGAGAAGTAATTGATGCACTGGGGCCAAATGGAATTGTTATCAACGTTGGACGAGGTGCTCATGTCAATGAATCTGAGCTTGTCTCAGCATTACTTGAAGGTCGTTTGGCAGGGGCAGGACTTGATGTATTTGAAAACGAGCCCCATGTACCTGAACAGTTGTTTGAGCTTGAAAACGTAGTTCTTCTGCCTCACGTGGGAACTGATACCATGGAGACCAGCACCGCAATGGCAGACCTTGTAATTAGAAACTTGGAGGCACATTTCAGGAATGAGCCTCTGATTACTCCTGTGATT

Protein sequence

MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADANLIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHSGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRNLEAHFRNEPLITPVI
Homology
BLAST of MS009488 vs. NCBI nr
Match: XP_022155087.1 (uncharacterized protein LOC111022216 [Momordica charantia])

HSP 1 Score: 624.8 bits (1610), Expect = 4.1e-175
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN
Sbjct: 334 MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 393

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC
Sbjct: 394 LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 453

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH
Sbjct: 454 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 513

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE
Sbjct: 514 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 573

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSALLEGRLAGAGLDVFENEPHVPEQLFELE VVLLPHVGTDTMETSTAMADLVIRN
Sbjct: 574 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 633

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 634 LEAHFRNEPLITPVI 648

BLAST of MS009488 vs. NCBI nr
Match: XP_038897872.1 (glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR-like [Benincasa hispida])

HSP 1 Score: 539.7 bits (1389), Expect = 1.7e-149
Identity = 272/315 (86.35%), Postives = 290/315 (92.06%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGVAMTTPMF YLEQQLE+RF LFKLWNHPL+SD+++ENA SIRAVVG TKSGADAN
Sbjct: 1   MESIGVAMTTPMFSYLEQQLERRFNLFKLWNHPLDSDFLQENAQSIRAVVGCTKSGADAN 60

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI SLPALEIVA+FSVGLDKIDLPKC EKGIRV NTPDVLTDDVADAAIGLAMAVSRRIC
Sbjct: 61  LIDSLPALEIVATFSVGLDKIDLPKCMEKGIRVVNTPDVLTDDVADAAIGLAMAVSRRIC 120

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFVRSGSW + GFRLAT+F+GKSVGILGLGRIGSAIAKR EAFGCPI Y +R + H 
Sbjct: 121 ESDRFVRSGSWTKDGFRLATRFNGKSVGILGLGRIGSAIAKRAEAFGCPIGYFTRRKMHQ 180

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SG  YKYF + +DLAANSQILFV+CTLTEETKHIVNREVID LGPNGI+INVGRGAH++E
Sbjct: 181 SG--YKYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDTLGPNGILINVGRGAHIDE 240

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSALLEGRL GAGLDVFENEPHVPEQL ELENVVLLPHVGTDT+ETS AMADLVI N
Sbjct: 241 SELVSALLEGRLGGAGLDVFENEPHVPEQLLELENVVLLPHVGTDTIETSIAMADLVISN 300

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 301 LEAHFRNEPLITPVI 313

BLAST of MS009488 vs. NCBI nr
Match: KAG6593464.1 (Hydroxyphenylpyruvate reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 537.7 bits (1384), Expect = 6.6e-149
Identity = 269/315 (85.40%), Postives = 287/315 (91.11%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           MEKIGVAMTTPMF YLE+QLEQRF LFKLW HPLNSDY+++NA SIRAVVG TK GADAN
Sbjct: 17  MEKIGVAMTTPMFSYLEKQLEQRFNLFKLWIHPLNSDYLQQNAKSIRAVVGCTKIGADAN 76

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI + PALEIVASFSVGLDKIDLPKC+EKGIRVTNTPDVLTDDVADAAIGLA+AVSRRIC
Sbjct: 77  LIDTFPALEIVASFSVGLDKIDLPKCREKGIRVTNTPDVLTDDVADAAIGLAIAVSRRIC 136

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFV+SGSW +GG  L TKF+GKSVGI+GLGRIGSAIAKR EAFGC ISYCSRNEKHH
Sbjct: 137 ESDRFVKSGSWTKGGLGLGTKFNGKSVGIVGLGRIGSAIAKRAEAFGCSISYCSRNEKHH 196

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SG  YKYF + +DLAANSQ LFVAC LT+ETKHI+NREVID LGPNGI+INVGRG HVNE
Sbjct: 197 SG--YKYFPSVVDLAANSQFLFVACNLTQETKHIINREVIDGLGPNGILINVGRGVHVNE 256

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSALLEGRL G GLDVFENEPHVPEQL ELEN VLLPHVGTDT+ET+ AMADLVI N
Sbjct: 257 SELVSALLEGRLGGVGLDVFENEPHVPEQLLELENAVLLPHVGTDTIETTIAMADLVIGN 316

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 317 LEAHFRNEPLITPVI 329

BLAST of MS009488 vs. NCBI nr
Match: XP_023514517.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111778775 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 537.0 bits (1382), Expect = 1.1e-148
Identity = 269/315 (85.40%), Postives = 288/315 (91.43%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           MEKIGVAMTTPMF YLE+QLEQRF LFKLW HPLNSDY+++NA SIRAVVG TK GADAN
Sbjct: 328 MEKIGVAMTTPMFSYLEEQLEQRFNLFKLWIHPLNSDYLQQNAKSIRAVVGCTKIGADAN 387

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI + PALEIVASFSVGLDKIDLPKC+EKGIRVTNTPDVLTDDVADAAIGLA+AVSRRIC
Sbjct: 388 LIDTFPALEIVASFSVGLDKIDLPKCREKGIRVTNTPDVLTDDVADAAIGLAIAVSRRIC 447

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFV+SGSW +GG  L TKF+GKSVGI+GLGRIGSAIAKR EAFGC ISYCSRNEKHH
Sbjct: 448 ESDRFVKSGSWTKGGLGLGTKFNGKSVGIVGLGRIGSAIAKRAEAFGCSISYCSRNEKHH 507

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SG  YKYF + +DLAANSQ LFVAC LT+ETKHI+NREVIDALGPNGI+INVGRGAHVNE
Sbjct: 508 SG--YKYFPSVVDLAANSQFLFVACNLTQETKHIINREVIDALGPNGILINVGRGAHVNE 567

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSAL +GRL G GLDVFENEPHVPEQL ELEN VLLPHVGTDT+ET+ AMADLVI N
Sbjct: 568 SELVSALHKGRLGGVGLDVFENEPHVPEQLLELENAVLLPHVGTDTIETTIAMADLVIGN 627

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 628 LEAHFRNEPLITPVI 640

BLAST of MS009488 vs. NCBI nr
Match: XP_023000640.1 (uncharacterized protein LOC111494888 [Cucurbita maxima])

HSP 1 Score: 535.4 bits (1378), Expect = 3.3e-148
Identity = 268/315 (85.08%), Postives = 288/315 (91.43%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           MEKIGVAMTTPMF YLE+QLEQRF LFKLW HPLNSDY+++NA SIRAVVG TK GADAN
Sbjct: 350 MEKIGVAMTTPMFTYLEKQLEQRFNLFKLWIHPLNSDYLQQNAKSIRAVVGCTKIGADAN 409

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI + PALEIVASFSVG+DKIDLPKC+ KGIRVTNTP+VLTDDVADAAIGLA+AVSRRIC
Sbjct: 410 LIDTFPALEIVASFSVGIDKIDLPKCRVKGIRVTNTPEVLTDDVADAAIGLAIAVSRRIC 469

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFV+SGSW +GG  L TKF+GKSVGI+GLGRIGSAIAKR EAFGC ISYCSRNEKHH
Sbjct: 470 ESDRFVKSGSWTKGGLGLGTKFNGKSVGIVGLGRIGSAIAKRAEAFGCSISYCSRNEKHH 529

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SG  YKYF + +DLAANSQ LFVAC LT+ETKHI+NREVIDALGPNGI+INVGRGAHVNE
Sbjct: 530 SG--YKYFPSVVDLAANSQFLFVACNLTQETKHIINREVIDALGPNGILINVGRGAHVNE 589

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSALLEGRL G GLDVFENEPHVPEQL ELEN VLLPHVGTDT+ET+ AMADLVI N
Sbjct: 590 SELVSALLEGRLGGVGLDVFENEPHVPEQLLELENAVLLPHVGTDTIETTIAMADLVIGN 649

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 650 LEAHFRNEPLITPVI 662

BLAST of MS009488 vs. ExPASy Swiss-Prot
Match: A5CAL1 (Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN=2KGR PE=1 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.4e-122
Identity = 216/315 (68.57%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGV +T PM  YLEQ+L++RFKLF+ W+ P  +D  RE+++SIRAVVG +  GADA 
Sbjct: 1   MESIGVLLTCPMNPYLEQELDKRFKLFRFWDFPSANDLFREHSNSIRAVVGNSFIGADAQ 60

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           +I +LP +EIV+SFSVGLDKIDL +C+EKGIRVTNTPDVLT+DVAD A+ L +A  RRIC
Sbjct: 61  MIEALPKMEIVSSFSVGLDKIDLVRCKEKGIRVTNTPDVLTEDVADLALALILATLRRIC 120

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDR+VRSGSWK+G F+L TKF+GKSVGI+GLGRIGSAIAKR E F CPISY SR EK  
Sbjct: 121 ESDRYVRSGSWKKGDFKLTTKFTGKSVGIIGLGRIGSAIAKRAEGFSCPISYHSRTEK-- 180

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
            G NYKY+ + ++LA+N QIL VAC LT ET+HI+NREVI+ALGP G+VIN+GRG HV+E
Sbjct: 181 PGTNYKYYPSVVELASNCQILVVACALTPETRHIINREVINALGPKGVVINIGRGLHVDE 240

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
            ELVSAL+EGRL GAGLDVFENEP+VPE+L  ++NVVLLPHVG+ T+ET   MADLV+ N
Sbjct: 241 PELVSALVEGRLGGAGLDVFENEPNVPEELLAMDNVVLLPHVGSGTVETRKDMADLVLGN 300

Query: 301 LEAHFRNEPLITPVI 316
           LEAHF N+PL+TPV+
Sbjct: 301 LEAHFLNKPLLTPVV 313

BLAST of MS009488 vs. ExPASy Swiss-Prot
Match: Q65CJ7 (Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR PE=1 SV=2)

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-119
Identity = 212/315 (67.30%), Postives = 255/315 (80.95%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGV M  PM  YLEQ+L++RFKLF+ W  P   D++   A SIRAVVG + +GADA 
Sbjct: 1   MEAIGVLMMCPMSTYLEQELDKRFKLFRYWTQPAQRDFLALQAESIRAVVGNSNAGADAE 60

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI +LP LEIV+SFSVGLDK+DL KC+EKG+RVTNTPDVLTDDVAD AIGL +AV RRIC
Sbjct: 61  LIDALPKLEIVSSFSVGLDKVDLIKCEEKGVRVTNTPDVLTDDVADLAIGLILAVLRRIC 120

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           E D++VR G+WK G F+L TKFSGK VGI+GLGRIG A+A+R EAF CPISY SR++K +
Sbjct: 121 ECDKYVRRGAWKFGDFKLTTKFSGKRVGIIGLGRIGLAVAERAEAFDCPISYFSRSKKPN 180

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           +  NY Y+ + ++LA+NS IL VAC LT ET HI+NREVIDALGP G++IN+GRG HV+E
Sbjct: 181 T--NYTYYGSVVELASNSDILVVACPLTPETTHIINREVIDALGPKGVLINIGRGPHVDE 240

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
            ELVSAL+EGRL GAGLDVFE EP VPE+LF LENVVLLPHVG+ T+ET   MADLV+ N
Sbjct: 241 PELVSALVEGRLGGAGLDVFEREPEVPEKLFGLENVVLLPHVGSGTVETRKVMADLVVGN 300

Query: 301 LEAHFRNEPLITPVI 316
           LEAHF  +PL+TPV+
Sbjct: 301 LEAHFSGKPLLTPVV 313

BLAST of MS009488 vs. ExPASy Swiss-Prot
Match: Q9CA90 (Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=HPR2 PE=1 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 3.8e-115
Identity = 208/315 (66.03%), Postives = 246/315 (78.10%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGV M  PM  YLE +LE+RF L + W  P  S  +  + +SIRAVVG   +GADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI  LP LEIV+SFSVGLDKIDL KC+EKGIRVTNTPDVLT+DVAD AIGL +A+ RR+C
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           E DR+VRSG WK+G F+L TKFSGKSVGI+GLGRIG+AIAKR EAF CPI+Y SR  K  
Sbjct: 121 ECDRYVRSGKWKQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIK-- 180

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
               YKY+   +DLA NS IL VAC LTE+T+HIV+R+V+DALG  G++IN+GRG HV+E
Sbjct: 181 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 240

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
            EL+ AL EGRL GA LDVFE EPHVPE+LF LENVVLLPHVG+ T+ET  AMADLV+ N
Sbjct: 241 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 300

Query: 301 LEAHFRNEPLITPVI 316
           LEAHF  + L+TPV+
Sbjct: 301 LEAHFSGKSLLTPVV 313

BLAST of MS009488 vs. ExPASy Swiss-Prot
Match: Q9LE33 (Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR3 PE=2 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 8.8e-72
Identity = 143/307 (46.58%), Postives = 205/307 (66.78%), Query Frame = 0

Query: 11  PMFDYLEQQLEQRFKLFKLWNHPLNS--DYVRENAHSIRAVVGGTKSGADANLIHSLPAL 70
           P   ++++ L + F+          S   +   +A S RA V   +      L+  LP+L
Sbjct: 16  PSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVISGRLPVTDELLSHLPSL 75

Query: 71  EIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRS 130
           +I+   SVG+D IDL  C+ +GI +TN  +  +DDVAD A+GL ++V RRI  +DR+VRS
Sbjct: 76  QILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRRIPAADRYVRS 135

Query: 131 GSW-KEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHSGYNYKY 190
           G+W K G F+L +K SGK VGI+GLG IGS +AKR+E+FGC ISY SR++K  S   Y+Y
Sbjct: 136 GNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQKQSS--PYRY 195

Query: 191 FSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSAL 250
           +S+ + LA N+ +L + C+LT+ET HIVNREV++ LG +G+VINVGRG  ++E E+V  L
Sbjct: 196 YSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLIDEKEMVKCL 255

Query: 251 LEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRNLEAHFRN 310
           ++G + GAGLDVFENEP VP++LF L+NVVL PH    T  +   +A + + NL+A F N
Sbjct: 256 VDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALANLKAFFSN 315

Query: 311 EPLITPV 315
            PL++PV
Sbjct: 316 RPLLSPV 320

BLAST of MS009488 vs. ExPASy Swiss-Prot
Match: Q9C4M5 (Glyoxylate reductase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) OX=523849 GN=gyaR PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 9.8e-55
Identity = 126/299 (42.14%), Postives = 174/299 (58.19%), Query Frame = 0

Query: 22  QRFKLFKLWNHPL--NSDYVRENAHSIRAVVGGTKSGADANLIHSLPALEIVASFSVGLD 81
           ++F   +LW  P       + E    + A+V       D  L+ + P L+I+A ++VG D
Sbjct: 20  EKFYEIELWKDPKAPPRGVLLEKVREVDALVTLVTDKVDKELLENAPKLKIIAQYAVGYD 79

Query: 82  KIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRSGSWK--EGGFR 141
            ID+ +  ++GI VTNTP VLTD  AD A  L +AV+RRI E+D FVRSG WK  E G+ 
Sbjct: 80  NIDIEEATKRGIYVTNTPGVLTDATADLAFALLLAVARRIVEADAFVRSGEWKKSEVGWH 139

Query: 142 ----LATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHS----GYNYKYFS 201
               L     GK++GI+G GRIG A+AKR + FG  I Y SR  K  +    G  Y  F 
Sbjct: 140 PLMFLGYGLKGKTLGIVGFGRIGQALAKRAKGFGMKIIYYSRTRKPEAEEEIGAEYVDFE 199

Query: 202 NAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSALLE 261
               L   S  + +   LT+ET H++  + +  + PN I+IN  RGA V+ + L+ AL E
Sbjct: 200 T---LLKESDFISLHVPLTKETYHMIGEKELKLMKPNAILINTSRGAVVDTNALIKALKE 259

Query: 262 GRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRNLEAHFRNE 309
           G +AGAGLDVFE EP+  E+LF+L+NVVL PH+G+ T E    MA+LV +NL A  + E
Sbjct: 260 GWIAGAGLDVFEEEPYYNEELFKLKNVVLAPHIGSATHEAREGMAELVAKNLIAFAKGE 315

BLAST of MS009488 vs. ExPASy TrEMBL
Match: A0A6J1DNF0 (uncharacterized protein LOC111022216 OS=Momordica charantia OX=3673 GN=LOC111022216 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.0e-175
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN
Sbjct: 334 MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 393

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC
Sbjct: 394 LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 453

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH
Sbjct: 454 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 513

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE
Sbjct: 514 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 573

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSALLEGRLAGAGLDVFENEPHVPEQLFELE VVLLPHVGTDTMETSTAMADLVIRN
Sbjct: 574 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELEKVVLLPHVGTDTMETSTAMADLVIRN 633

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 634 LEAHFRNEPLITPVI 648

BLAST of MS009488 vs. ExPASy TrEMBL
Match: A0A6J1KIX6 (uncharacterized protein LOC111494888 OS=Cucurbita maxima OX=3661 GN=LOC111494888 PE=4 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.6e-148
Identity = 268/315 (85.08%), Postives = 288/315 (91.43%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           MEKIGVAMTTPMF YLE+QLEQRF LFKLW HPLNSDY+++NA SIRAVVG TK GADAN
Sbjct: 350 MEKIGVAMTTPMFTYLEKQLEQRFNLFKLWIHPLNSDYLQQNAKSIRAVVGCTKIGADAN 409

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI + PALEIVASFSVG+DKIDLPKC+ KGIRVTNTP+VLTDDVADAAIGLA+AVSRRIC
Sbjct: 410 LIDTFPALEIVASFSVGIDKIDLPKCRVKGIRVTNTPEVLTDDVADAAIGLAIAVSRRIC 469

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFV+SGSW +GG  L TKF+GKSVGI+GLGRIGSAIAKR EAFGC ISYCSRNEKHH
Sbjct: 470 ESDRFVKSGSWTKGGLGLGTKFNGKSVGIVGLGRIGSAIAKRAEAFGCSISYCSRNEKHH 529

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SG  YKYF + +DLAANSQ LFVAC LT+ETKHI+NREVIDALGPNGI+INVGRGAHVNE
Sbjct: 530 SG--YKYFPSVVDLAANSQFLFVACNLTQETKHIINREVIDALGPNGILINVGRGAHVNE 589

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSALLEGRL G GLDVFENEPHVPEQL ELEN VLLPHVGTDT+ET+ AMADLVI N
Sbjct: 590 SELVSALLEGRLGGVGLDVFENEPHVPEQLLELENAVLLPHVGTDTIETTIAMADLVIGN 649

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 650 LEAHFRNEPLITPVI 662

BLAST of MS009488 vs. ExPASy TrEMBL
Match: A0A5D3BWA0 (Hydroxyphenylpyruvate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002220 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 3.4e-143
Identity = 265/315 (84.13%), Postives = 282/315 (89.52%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGVAMTTPMF YLEQ LE+RF LFKLWNHPL+SD+++ NA SIRAVVG T SGADA+
Sbjct: 1   MESIGVAMTTPMFGYLEQNLERRFNLFKLWNHPLDSDFLQHNAQSIRAVVGCTTSGADAH 60

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI + P LEIVA+FSVGLDKIDLPKC EKGIRV NTPDVLTDDVADAAIGLAMAV RRI 
Sbjct: 61  LIDTFPNLEIVATFSVGLDKIDLPKCLEKGIRVVNTPDVLTDDVADAAIGLAMAVLRRIS 120

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFVRSGSW +  F LAT+F+GKSVGILGLGRIGSAIAKR EAFGC I Y SR EKHH
Sbjct: 121 ESDRFVRSGSWMKDEFGLATRFNGKSVGILGLGRIGSAIAKRAEAFGCNIGYFSRTEKHH 180

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SG  YKYF + +DLAANSQILFV+CTLTEETKHIVNREVIDALGPNGI+INVGRGAHVNE
Sbjct: 181 SG--YKYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDALGPNGILINVGRGAHVNE 240

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSALLE RL GAGLDVFENEPHVPE+L EL NVVLLPHVGTDT+ETS AMADLVIRN
Sbjct: 241 SELVSALLERRLGGAGLDVFENEPHVPEKLLELNNVVLLPHVGTDTIETSIAMADLVIRN 300

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 301 LEAHFRNEPLITPVI 313

BLAST of MS009488 vs. ExPASy TrEMBL
Match: A0A1S3CG96 (hydroxyphenylpyruvate reductase-like OS=Cucumis melo OX=3656 GN=LOC103500587 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 3.4e-143
Identity = 265/315 (84.13%), Postives = 282/315 (89.52%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGVAMTTPMF YLEQ LE+RF LFKLWNHPL+SD+++ NA SIRAVVG T SGADA+
Sbjct: 23  MESIGVAMTTPMFGYLEQNLERRFNLFKLWNHPLDSDFLQHNAQSIRAVVGCTTSGADAH 82

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI + P LEIVA+FSVGLDKIDLPKC EKGIRV NTPDVLTDDVADAAIGLAMAV RRI 
Sbjct: 83  LIDTFPNLEIVATFSVGLDKIDLPKCLEKGIRVVNTPDVLTDDVADAAIGLAMAVLRRIS 142

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFVRSGSW +  F LAT+F+GKSVGILGLGRIGSAIAKR EAFGC I Y SR EKHH
Sbjct: 143 ESDRFVRSGSWMKDEFGLATRFNGKSVGILGLGRIGSAIAKRAEAFGCNIGYFSRTEKHH 202

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
           SG  YKYF + +DLAANSQILFV+CTLTEETKHIVNREVIDALGPNGI+INVGRGAHVNE
Sbjct: 203 SG--YKYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDALGPNGILINVGRGAHVNE 262

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           SELVSALLE RL GAGLDVFENEPHVPE+L EL NVVLLPHVGTDT+ETS AMADLVIRN
Sbjct: 263 SELVSALLERRLGGAGLDVFENEPHVPEKLLELNNVVLLPHVGTDTIETSIAMADLVIRN 322

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 323 LEAHFRNEPLITPVI 335

BLAST of MS009488 vs. ExPASy TrEMBL
Match: A0A0A0K6M4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446950 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 2.4e-141
Identity = 261/315 (82.86%), Postives = 278/315 (88.25%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGVAMTT MF YLEQ LE RF LFKLWNHPL+SD+++ NA S+RAVVG TKSGADA 
Sbjct: 1   MESIGVAMTTQMFTYLEQNLEGRFNLFKLWNHPLDSDFLQLNAQSVRAVVGCTKSGADAR 60

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI + P LEIVA+FSVGLDKIDLPKC EKGIRV NTPDVLTDDVADAAIGLAMAV RRI 
Sbjct: 61  LIDTFPNLEIVATFSVGLDKIDLPKCLEKGIRVVNTPDVLTDDVADAAIGLAMAVLRRIS 120

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           ESDRFVRSGSW +  F L T+F+GKSVGI+GLGRIGSAIAKR  AFGCPI Y SR EKHH
Sbjct: 121 ESDRFVRSGSWMKNDFGLGTRFNGKSVGIIGLGRIGSAIAKRAVAFGCPIGYFSRTEKHH 180

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
            G  YKYF + +DLAANSQILFV+CTLTEETKHIVNREVIDALGPNGI+INVGRGAHVNE
Sbjct: 181 RG--YKYFPSVLDLAANSQILFVSCTLTEETKHIVNREVIDALGPNGILINVGRGAHVNE 240

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
           +ELVSALLE RL GAGLDVFENEPHVPEQL EL NVVLLPHVGTDT+ETS AMADLVIRN
Sbjct: 241 TELVSALLERRLGGAGLDVFENEPHVPEQLLELNNVVLLPHVGTDTIETSIAMADLVIRN 300

Query: 301 LEAHFRNEPLITPVI 316
           LEAHFRNEPLITPVI
Sbjct: 301 LEAHFRNEPLITPVI 313

BLAST of MS009488 vs. TAIR 10
Match: AT1G79870.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 416.0 bits (1068), Expect = 2.7e-116
Identity = 208/315 (66.03%), Postives = 246/315 (78.10%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGV M  PM  YLE +LE+RF L + W  P  S  +  + +SIRAVVG   +GADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI  LP LEIV+SFSVGLDKIDL KC+EKGIRVTNTPDVLT+DVAD AIGL +A+ RR+C
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           E DR+VRSG WK+G F+L TKFSGKSVGI+GLGRIG+AIAKR EAF CPI+Y SR  K  
Sbjct: 121 ECDRYVRSGKWKQGEFQLTTKFSGKSVGIIGLGRIGTAIAKRAEAFSCPINYYSRTIK-- 180

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
               YKY+   +DLA NS IL VAC LTE+T+HIV+R+V+DALG  G++IN+GRG HV+E
Sbjct: 181 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 240

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
            EL+ AL EGRL GA LDVFE EPHVPE+LF LENVVLLPHVG+ T+ET  AMADLV+ N
Sbjct: 241 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 300

Query: 301 LEAHFRNEPLITPVI 316
           LEAHF  + L+TPV+
Sbjct: 301 LEAHFSGKSLLTPVV 313

BLAST of MS009488 vs. TAIR 10
Match: AT1G79870.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 375.9 bits (964), Expect = 3.1e-104
Identity = 193/315 (61.27%), Postives = 229/315 (72.70%), Query Frame = 0

Query: 1   MEKIGVAMTTPMFDYLEQQLEQRFKLFKLWNHPLNSDYVRENAHSIRAVVGGTKSGADAN 60
           ME IGV M  PM  YLE +LE+RF L + W  P  S  +  + +SIRAVVG   +GADA 
Sbjct: 1   MESIGVLMMCPMSSYLENELEKRFNLLRFWTSPEKSVLLETHRNSIRAVVGNASAGADAQ 60

Query: 61  LIHSLPALEIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRIC 120
           LI  LP LEIV+SFSVGLDKIDL KC+EKGIRVTNTPDVLT+DVAD AIGL +A+ RR+C
Sbjct: 61  LISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADLAIGLILALLRRLC 120

Query: 121 ESDRFVRSGSWKEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHH 180
           E DR+VRSG WK+                   GRIG+AIAKR EAF CPI+Y SR  K  
Sbjct: 121 ECDRYVRSGKWKQ-------------------GRIGTAIAKRAEAFSCPINYYSRTIK-- 180

Query: 181 SGYNYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNE 240
               YKY+   +DLA NS IL VAC LTE+T+HIV+R+V+DALG  G++IN+GRG HV+E
Sbjct: 181 PDVAYKYYPTVVDLAQNSDILVVACPLTEQTRHIVDRQVMDALGAKGVLINIGRGPHVDE 240

Query: 241 SELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRN 300
            EL+ AL EGRL GA LDVFE EPHVPE+LF LENVVLLPHVG+ T+ET  AMADLV+ N
Sbjct: 241 QELIKALTEGRLGGAALDVFEQEPHVPEELFGLENVVLLPHVGSGTVETRNAMADLVVGN 294

Query: 301 LEAHFRNEPLITPVI 316
           LEAHF  + L+TPV+
Sbjct: 301 LEAHFSGKSLLTPVV 294

BLAST of MS009488 vs. TAIR 10
Match: AT1G12550.1 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 271.9 bits (694), Expect = 6.3e-73
Identity = 143/307 (46.58%), Postives = 205/307 (66.78%), Query Frame = 0

Query: 11  PMFDYLEQQLEQRFKLFKLWNHPLNS--DYVRENAHSIRAVVGGTKSGADANLIHSLPAL 70
           P   ++++ L + F+          S   +   +A S RA V   +      L+  LP+L
Sbjct: 16  PSLTFMDEILTREFRTLITDTSSSESLPSFFPRHASSARAFVISGRLPVTDELLSHLPSL 75

Query: 71  EIVASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRS 130
           +I+   SVG+D IDL  C+ +GI +TN  +  +DDVAD A+GL ++V RRI  +DR+VRS
Sbjct: 76  QILVCTSVGIDHIDLAACKRRGIVITNAGNAFSDDVADCAVGLLISVLRRIPAADRYVRS 135

Query: 131 GSW-KEGGFRLATKFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHSGYNYKY 190
           G+W K G F+L +K SGK VGI+GLG IGS +AKR+E+FGC ISY SR++K  S   Y+Y
Sbjct: 136 GNWAKFGDFQLGSKVSGKRVGIVGLGSIGSFVAKRLESFGCVISYNSRSQKQSS--PYRY 195

Query: 191 FSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSAL 250
           +S+ + LA N+ +L + C+LT+ET HIVNREV++ LG +G+VINVGRG  ++E E+V  L
Sbjct: 196 YSDILSLAENNDVLVLCCSLTDETHHIVNREVMELLGKDGVVINVGRGKLIDEKEMVKCL 255

Query: 251 LEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRNLEAHFRN 310
           ++G + GAGLDVFENEP VP++LF L+NVVL PH    T  +   +A + + NL+A F N
Sbjct: 256 VDGVIGGAGLDVFENEPAVPQELFGLDNVVLSPHFAVATPGSLDNVAQIALANLKAFFSN 315

Query: 311 EPLITPV 315
            PL++PV
Sbjct: 316 RPLLSPV 320

BLAST of MS009488 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 252.3 bits (643), Expect = 5.2e-67
Identity = 126/295 (42.71%), Postives = 193/295 (65.42%), Query Frame = 0

Query: 23  RFKLFKLWNHPLN-SDYVRENAHSIRAVVGGTKSGADANLIHSLPALEIVASFSVGLDKI 82
           +F++ K +  PL   +++  ++ SI A++    +   A+LI  LP L +V + S G+D +
Sbjct: 45  KFEILKAFESPLPLPEFLAYHSDSISAIIAPVAAPVTADLIRILPNLRLVVTTSAGVDHV 104

Query: 83  DLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRSGSWK-EGGFRLAT 142
           DL +C+ +GI V N     ++DVAD A+GL + V RRI  ++RFV+   W  +G + L +
Sbjct: 105 DLVECRRRGISVANAGSSFSEDVADTAVGLLIDVFRRISAANRFVKQRFWPLKGDYPLGS 164

Query: 143 KFSGKSVGILGLGRIGSAIAKRVEAFGCPISYCSRNEKHHSGYNYKYFSNAIDLAANSQI 202
           K   K +GI+GLG IGS +A R++AFGC ISY SRN K +    Y Y+ +  ++AANS  
Sbjct: 165 KLGRKRIGIVGLGSIGSKVATRLDAFGCQISYSSRNRKPYD-VPYHYYMDIEEMAANSDA 224

Query: 203 LFVACTLTEETKHIVNREVIDALGPNGIVINVGRGAHVNESELVSALLEGRLAGAGLDVF 262
           L + C L E+T  ++N++V+ ALG  G+++NV RGA ++E E+V  L EG + GAGLDVF
Sbjct: 225 LIICCELNEKTLRLINKDVLSALGKRGVIVNVARGAIIDEEEMVRCLREGEIGGAGLDVF 284

Query: 263 ENEPHVPEQLFELENVVLLPHVGTDTMETSTAMADLVIRNLEAHFRNEPLITPVI 316
           E+EP+VP++LFEL+NVV  PH    T+E    +  +V+ N+EA F N+PL+TPV+
Sbjct: 285 EDEPNVPKELFELDNVVFSPHSAFMTLEGLEELGKVVVGNIEAFFSNKPLLTPVL 338

BLAST of MS009488 vs. TAIR 10
Match: AT1G68010.1 (hydroxypyruvate reductase )

HSP 1 Score: 124.8 bits (312), Expect = 1.2e-28
Identity = 80/260 (30.77%), Postives = 134/260 (51.54%), Query Frame = 0

Query: 72  ASFSVGLDKIDLPKCQEKGIRVTNTPDVLTDDVADAAIGLAMAVSRRICESDRFVRSG-- 131
           ++ +VG + +D+    + GI V NTP VLT+  A+ A  L++A +RRI E+D F+R G  
Sbjct: 91  SNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGGLY 150

Query: 132 -SWKEGGFRLATKFSGKSVGILGLGRIGSAIAK-RVEAFGCPISY-----CSRNEKHHSG 191
             W    F +     G++VG++G GRIGSA A+  VE F   + Y      +R EK  + 
Sbjct: 151 EGWLPHLF-VGNLLKGQTVGVIGAGRIGSAYARMMVEGFKMNLIYFDLYQSTRLEKFVTA 210

Query: 192 Y------------NYKYFSNAIDLAANSQILFVACTLTEETKHIVNREVIDALGPNGIVI 251
           Y             +K  S+  ++   + ++ +   L + T H+VN+E +  +    I++
Sbjct: 211 YGQFLKANGEQPVTWKRASSMEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKEAILV 270

Query: 252 NVGRGAHVNESELVSALLEGRLAGAGLDVFENEPHVPEQLFELENVVLLPHVGTDTMETS 311
           N  RG  ++E+ LV  L E  +   GLDVFE EP +   L + +N +++PH+ + +  T 
Sbjct: 271 NCSRGPVIDEAALVEHLKENPMFRVGLDVFEEEPFMKPGLADTKNAIVVPHIASASKWTR 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155087.14.1e-17599.68uncharacterized protein LOC111022216 [Momordica charantia][more]
XP_038897872.11.7e-14986.35glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR-like [Benincasa hispida][more]
KAG6593464.16.6e-14985.40Hydroxyphenylpyruvate reductase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023514517.11.1e-14885.40LOW QUALITY PROTEIN: uncharacterized protein LOC111778775 [Cucurbita pepo subsp.... [more]
XP_023000640.13.3e-14885.08uncharacterized protein LOC111494888 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A5CAL11.4e-12268.57Glyoxylate/hydroxypyruvate/pyruvate reductase 2KGR OS=Vitis vinifera OX=29760 GN... [more]
Q65CJ71.5e-11967.30Hydroxyphenylpyruvate reductase OS=Plectranthus scutellarioides OX=4142 GN=HPPR ... [more]
Q9CA903.8e-11566.03Glyoxylate/hydroxypyruvate reductase A HPR2 OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Q9LE338.8e-7246.58Glyoxylate/hydroxypyruvate reductase HPR3 OS=Arabidopsis thaliana OX=3702 GN=HPR... [more]
Q9C4M59.8e-5542.14Glyoxylate reductase OS=Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / J... [more]
Match NameE-valueIdentityDescription
A0A6J1DNF02.0e-17599.68uncharacterized protein LOC111022216 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1KIX61.6e-14885.08uncharacterized protein LOC111494888 OS=Cucurbita maxima OX=3661 GN=LOC111494888... [more]
A0A5D3BWA03.4e-14384.13Hydroxyphenylpyruvate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CG963.4e-14384.13hydroxyphenylpyruvate reductase-like OS=Cucumis melo OX=3656 GN=LOC103500587 PE=... [more]
A0A0A0K6M42.4e-14182.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446950 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G79870.12.7e-11666.03D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G79870.23.1e-10461.27D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G12550.16.3e-7346.58D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT2G45630.25.2e-6742.71D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G68010.11.2e-2830.77hydroxypyruvate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 109..283
e-value: 3.8E-50
score: 169.7
NoneNo IPR availableGENE3D3.40.50.720coord: 101..286
e-value: 2.3E-123
score: 412.6
NoneNo IPR availableGENE3D3.40.50.720coord: 17..309
e-value: 2.3E-123
score: 412.6
NoneNo IPR availablePANTHERPTHR10996:SF235D-GLYCERATE DEHYDROGENASE/HYDROXYPYRUVATE REDUCTASEcoord: 1..315
NoneNo IPR availablePANTHERPTHR109962-HYDROXYACID DEHYDROGENASE-RELATEDcoord: 1..315
NoneNo IPR availableCDDcd12156HPPRcoord: 4..306
e-value: 5.64222E-162
score: 451.538
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 4..136
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 14..314
e-value: 3.0E-21
score: 75.8
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 101..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009488.1MS009488.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor