MS009455 (gene) Bitter gourd (TR) v1

Overview
NameMS009455
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAcyl-[acyl-carrier-protein] hydrolase
Locationscaffold813: 1412738 .. 1415140 (-)
RNA-Seq ExpressionMS009455
SyntenyMS009455
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATATGGCAGTGATGTCTTCATCAAATATTTGTAATTTTGGTAAGGAAATAATCACAATTTCGAAGAGAGAACAAAGTTTTGGACATGCAATAAGAGGCAATTTGCAGCTCAAAGGCCATGCCCAATATTCCTCTCTACGCCTCAAAACTAACATTGCTCAAATCACTGCATGTAAGATGATGATGATGAAAAATCCTGCTTTCTCCAACTTTTTTTGGGGTTTTTTGAGAGATGATTTATTGATTTTTTTGTTGTTTTTGGTGGCAGGTGGGTTTAAAAATATTGATAGTCGTAACCCGAAAAAACTTGACATGACTATTGACGACTCGAGCATGGGAGAGCTATTAAACAATGGTCAGATTTATCAACATCATTTCTTGATCAAAGCATACGAGTCGGGAGTTGATAAAAATATGGCCATGTTTTTCATTATCAACCAATTACAGGTTTGGATCCAACATTAAACTTATAAGAATAATTATTAATGTATCTTAACCCGTCAAATTATATTTCGCTTGACATAAAAATTTTGTGAGTTTTTTCTTATTAGGAGTCAAAACTAAACCACTTGAAAAGTTGTGGGTTGGTGAATGGAGGATTGGGAACAACTATAGAAATGGATAAAAGGGACCTCTTATGGGTGGTTCATAAAATGCAAGTGGTAGTCGACCACTATCCTTCTTGGTAATTATAATTTTTCTTTTTAAATTCCATAATTTAAAAAAACAATAGCCATATTGAATAATTTATCGATTCATTTTCATCTTGGGTAGGAAAATGTATACAAGTATATGTAAATGTGTGTTTGGTTGTATTTTTTTTAGGAAGGATGTTGTTCAACTAAGCACTTGGAGTTGTATTCATGGGAAGCATGGTTATCGTCGAGATTGGATCATTCGAGATCATAAAACTGGCGAAGTTTTGATTCGTGCAATCTGGTCTGTTCTTTTCTTCTCTTTCTACTTTAAACATAAGAAAAACGAAAATAAATTCGACGTTAATTTATGTTTGCATATATTGCAGTTTCTATGCTGTGATGAACAGGAAAACAAGAAAATTATCAAAGTTTTGTGATGAAATGAAGACCGAAATAACTCCCTACTTTTTGGATTGTGATCCGATTATCGAAGAGGAGAAAATTATTTCAAATAAACTAGATGTTAATTCTGATGGTTCCCACATAACATGTTTAGTTGTAAGTACTTTTATTTAAAAAACGTATGATTCTATTTAATTAAGGTTATAGTTTTTATCTTTTAAACTTAGTAATAATTATCAACCGTTTTTTTTCCCTTAATTCTCAATTTTTCAAAATATTTTTGAAACTTTGGTGAAAAAAATTTTGCTTTTGTTCTTAAAAATTGATTCTTTAAAAAGTTAAAAAAACAAAACAAATAATTTTGAGCAAATTACAAGGTTAAAGGGTATGCCAATAATGATTCTTTAGGNNNNNNNNNNNNNNNNNNNAGGTTTTTGGGGATAAAAAAAAAAAAAAACTCATTTCTCATGGCATTTTGATTATTAGGTATTTGTTTCTTTAACGTTATATGTGTCAAATGGTTTAAATTTCATAATACTATTTTTTAAAATTTTATTATATTCTTAAACTTTCACGTTTATTTTTATTTACTCAGTAAGTCAAGTATTAGAAATTTCCAAATAGATTTAAATTCTTATTTAATAATGAATTCAGAATTAACAACACATACTAAAATAGTTTTTTTTTTTTTTTAACTCATAGACAAGGCCGAGTGATCTAGATATGAATCTACACGTTAACAATGCTAGTTATATCAACTTCGTCCTTGAGGTATGTTATCTCTTTTATCCTCTTTTTACCTTATTTTTTTATGGTAGTGGTTGAGCACAACAATCTTCTATATGTGTTATTACCCCTTTAATGGGTCAATTTTGATGCTAAATTAAAGTGTGAATTCTACCCAATAAATGATTTTTTTTAAAGAAGTTTATAGTAAAAAAATCAAGACCTACTCAAATTCAAATTAAAAAAATTATTCATGACCGGTGGAAGATCCAGTTATAGAAATACAAAAACCTTGCTAAAAAGATATTTCAACATAGTTTAAACTTTAAGGTTAAATTTGACTCAGTCCATTCAGATGGAACTTAAAAAAAAAAGTAAATTCTCTTAAAAATAGTAAATTTTGTTTCAGAGTACTCCTCGATGGTTGTTTGAGAATTATCAGCTGTCAACCGTAACAATGAAGTACCTCAGAGAATGTGGAATGGACAAGCAATTGCAGTCTCTCACTGTGCCCATGAAAAACGACATTAATGGCGATGACCACAACAAAAGAAAAGACATCAAGTTCCACCACTCCATTCTTGCAGATGGGATTGAACTTGTTCGAGGAAGAACCATTTGGACCCCTAAACCTACA

mRNA sequence

AATATGGCAGTGATGTCTTCATCAAATATTTGTAATTTTGGTAAGGAAATAATCACAATTTCGAAGAGAGAACAAAGTTTTGGACATGCAATAAGAGGCAATTTGCAGCTCAAAGGCCATGCCCAATATTCCTCTCTACGCCTCAAAACTAACATTGCTCAAATCACTGCATGTAAGATGATGATGATGAAAAATCCTGCTTTCTCCAACTTTTTTTGGGGTTTTTTGAGAGATGATTTATTGATTTTTTTGTTGTTTTTGGTGGCAGGTGGGTTTAAAAATATTGATAGTCGTAACCCGAAAAAACTTGACATGACTATTGACGACTCGAGCATGGGAGAGCTATTAAACAATGGTCAGATTTATCAACATCATTTCTTGATCAAAGCATACGAGTCGGGAGTTGATAAAAATATGGCCATGTTTTTCATTATCAACCAATTACAGACCGAAATAACTCCCTACTTTTTGGATTGTGATCCGATTATCGAAGAGGAGAAAATTATTTCAAATAAACTAGATGTTAATTCTGATGGTTCCCACATAACATGTTTAGTTACAAGGCCGAGTGATCTAGATATGAATCTACACGTTAACAATGCTAGTTATATCAACTTCGTCCTTGAGAGTACTCCTCGATGGTTGTTTGAGAATTATCAGCTGTCAACCGTAACAATGAAGTACCTCAGAGAATGTGGAATGGACAAGCAATTGCAGTCTCTCACTGTGCCCATGAAAAACGACATTAATGGCGATGACCACAACAAAAGAAAAGACATCAAGTTCCACCACTCCATTCTTGCAGATGGGATTGAACTTGTTCGAGGAAGAACCATTTGGACCCCTAAACCTACA

Coding sequence (CDS)

AATATGGCAGTGATGTCTTCATCAAATATTTGTAATTTTGGTAAGGAAATAATCACAATTTCGAAGAGAGAACAAAGTTTTGGACATGCAATAAGAGGCAATTTGCAGCTCAAAGGCCATGCCCAATATTCCTCTCTACGCCTCAAAACTAACATTGCTCAAATCACTGCATGTAAGATGATGATGATGAAAAATCCTGCTTTCTCCAACTTTTTTTGGGGTTTTTTGAGAGATGATTTATTGATTTTTTTGTTGTTTTTGGTGGCAGGTGGGTTTAAAAATATTGATAGTCGTAACCCGAAAAAACTTGACATGACTATTGACGACTCGAGCATGGGAGAGCTATTAAACAATGGTCAGATTTATCAACATCATTTCTTGATCAAAGCATACGAGTCGGGAGTTGATAAAAATATGGCCATGTTTTTCATTATCAACCAATTACAGACCGAAATAACTCCCTACTTTTTGGATTGTGATCCGATTATCGAAGAGGAGAAAATTATTTCAAATAAACTAGATGTTAATTCTGATGGTTCCCACATAACATGTTTAGTTACAAGGCCGAGTGATCTAGATATGAATCTACACGTTAACAATGCTAGTTATATCAACTTCGTCCTTGAGAGTACTCCTCGATGGTTGTTTGAGAATTATCAGCTGTCAACCGTAACAATGAAGTACCTCAGAGAATGTGGAATGGACAAGCAATTGCAGTCTCTCACTGTGCCCATGAAAAACGACATTAATGGCGATGACCACAACAAAAGAAAAGACATCAAGTTCCACCACTCCATTCTTGCAGATGGGATTGAACTTGTTCGAGGAAGAACCATTTGGACCCCTAAACCTACA

Protein sequence

NMAVMSSSNICNFGKEIITISKREQSFGHAIRGNLQLKGHAQYSSLRLKTNIAQITACKMMMMKNPAFSNFFWGFLRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQIYQHHFLIKAYESGVDKNMAMFFIINQLQTEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINFVLESTPRWLFENYQLSTVTMKYLRECGMDKQLQSLTVPMKNDINGDDHNKRKDIKFHHSILADGIELVRGRTIWTPKPT
Homology
BLAST of MS009455 vs. NCBI nr
Match: KAA0058801.1 (palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10596.1 palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa])

HSP 1 Score: 224.9 bits (572), Expect = 8.6e-55
Identity = 146/386 (37.82%), Postives = 188/386 (48.70%), Query Frame = 0

Query: 2   MAVMSSSNICNFGKEIITISKREQSFGHAIRGNLQLKGHAQYSSLRLKTNIAQITACKMM 61
           MA +SSS  CN GK+  +  K+EQ FG +  GNLQLK H +     L+T           
Sbjct: 1   MAAVSSSYCCNLGKKFNSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRT----------- 60

Query: 62  MMKNPAFSNFFWGFLRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQI 121
                  SN           I     +A   + I+S   + LD+T  + +MGELLN+G +
Sbjct: 61  -------SN-----------IIAKSSIASEVRKINSYESRNLDITHGNLTMGELLNDGLV 120

Query: 122 YQHHFLIKAYESGVDKNMAMFFIINQL--------------------------------- 181
           YQ HFL+K+YESG DK M MFFIINQL                                 
Sbjct: 121 YQRHFLVKSYESGPDKKMTMFFIINQLHESKLNHLKSCGLVTTGLGTTPEMTKRGLIWVV 180

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 181 HKLQVVVDQYPSWNDIVQLQTWSCFYGKNSYRRDWIVRNYKTGQVLIRASCFYVLMNRNT 240

Query: 242 ----------QTEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHV 283
                     + E+ P+FL  DPIIEEE IIS KLDV++ G + T LVTR SDLDMN HV
Sbjct: 241 RKLSKFYDEMKEEMNPHFLKSDPIIEEENIISTKLDVDAKGCNCTSLVTRLSDLDMNHHV 300

BLAST of MS009455 vs. NCBI nr
Match: KAG6593401.1 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 221.1 bits (562), Expect = 1.2e-53
Identity = 141/391 (36.06%), Postives = 188/391 (48.08%), Query Frame = 0

Query: 2   MAVMSSSNICNFGKEIITISKREQSFGHAIRGNLQLKGHAQYSSLRLKTNIAQITACKMM 61
           MA++ SSN  +FG E+++IS RE +FGHA   +L LKG  + +SL LK++          
Sbjct: 10  MALICSSNGSHFGMELVSISTREPTFGHATTPSLHLKGRPKSASLTLKSS---------- 69

Query: 62  MMKNPAFSNFFWGFLRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQI 121
            +  P                     +AGGFK+ID    +K ++T ++  + E LN+G +
Sbjct: 70  HISQP---------------------IAGGFKSIDDYMSRKPEITRENLMVEEFLNDGLV 129

Query: 122 YQHHFLIKAYESGVDKNMAMFFIINQLQ-------------------------------- 181
           YQ HFL+++YESG D  M MFF+INQLQ                                
Sbjct: 130 YQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRD 189

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 190 LIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVL 249

Query: 242 ----------------TEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLD 284
                            E+ P+FLD  PI+EE+ I+S KL  +        LVTR SDLD
Sbjct: 250 MNKRTRKLSRFCEELKEEMNPFFLDSHPILEEDNIVSQKLHFHQHYCTHASLVTRLSDLD 309

BLAST of MS009455 vs. NCBI nr
Match: KAG7025748.1 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 221.1 bits (562), Expect = 1.2e-53
Identity = 126/283 (44.52%), Postives = 166/283 (58.66%), Query Frame = 0

Query: 2   MAVMSSSNICNFGKEIITISKREQSFGHAIRGNLQLKGHAQYSSLRLKTNIAQITACKMM 61
           MA++ SSN  +FG E+++IS RE +FGHA   +L LKG  + +SL LK++          
Sbjct: 1   MALICSSNGSHFGMELVSISTREPTFGHATTPSLHLKGRPKSASLTLKSS---------- 60

Query: 62  MMKNPAFSNFFWGFLRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQI 121
            +  P                     +AGGFK ID    +K ++T ++  + E LN+G +
Sbjct: 61  HISQP---------------------IAGGFKRIDDYMSRKPEITRENLMVEEFLNDGLV 120

Query: 122 YQHHFLIKAYESGVDKNMAMFFIINQLQTEITPYFLDCDPIIEEEKIISNKLDVNSDGSH 181
           YQ HFL+++YESG D  M MFF+INQLQ                                
Sbjct: 121 YQCHFLVRSYESGPDNKMTMFFVINQLQ-------------------------------- 180

Query: 182 ITCLVTRPSDLDMNLHVNNASYINFVLESTPRWLFENYQLSTVTMKYLRECGMDKQLQSL 241
                TR SDLDMN H+NNASYINFVLE TPR + ENYQLS +T+KYL+EC +D+QLQSL
Sbjct: 181 -----TRLSDLDMNQHINNASYINFVLEGTPRAVLENYQLSGITIKYLKECSVDQQLQSL 215

Query: 242 TVPMKND-INGDDHNKRKDIKFHHSILADGIELVRGRTIWTPK 284
           +VP++ND +NG    K KDIK HHSIL+DGIELVR +T+WTPK
Sbjct: 241 SVPLENDVVNGTPLIKDKDIKLHHSILSDGIELVRAKTVWTPK 215

BLAST of MS009455 vs. NCBI nr
Match: XP_023514527.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 220.7 bits (561), Expect = 1.6e-53
Identity = 140/391 (35.81%), Postives = 188/391 (48.08%), Query Frame = 0

Query: 2   MAVMSSSNICNFGKEIITISKREQSFGHAIRGNLQLKGHAQYSSLRLKTNIAQITACKMM 61
           MA++ SSN  +FG E+++IS RE +FGHA   +L LK   + +SL LK++          
Sbjct: 1   MALICSSNGSHFGMELVSISMREPTFGHATTPSLHLKARPKTASLTLKSS---------- 60

Query: 62  MMKNPAFSNFFWGFLRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQI 121
            +  P                     +AGGFK+ID    +K ++T ++  + E LN+G +
Sbjct: 61  HISQP---------------------IAGGFKSIDDYVSRKPEITRENLMVEEFLNDGLV 120

Query: 122 YQHHFLIKAYESGVDKNMAMFFIINQLQ-------------------------------- 181
           YQ HFL+++YESG D  M MFF+INQLQ                                
Sbjct: 121 YQCHFLVRSYESGPDNKMTMFFVINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRD 180

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 181 LIWVVHKLQVVVDQFPSWNDVVELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVL 240

Query: 242 ----------------TEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLD 284
                            E+ P+FLD  PI+EE+ I+S KL ++        LVTR SDLD
Sbjct: 241 MNKRTRKLSKFCEELKEEMNPFFLDSHPILEEDNIVSQKLHLHQHDCTHASLVTRLSDLD 300

BLAST of MS009455 vs. NCBI nr
Match: XP_022960310.1 (palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-48
Identity = 132/378 (34.92%), Postives = 178/378 (47.09%), Query Frame = 0

Query: 16  EIITISKREQSFGHAIRGNLQLKGHAQYSSLRLK-TNIAQITACKMMMMKNPAFSNFFWG 75
           E+++IS RE +FGHA   +L LKG  + +SL LK ++I+Q T                  
Sbjct: 2   ELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPT------------------ 61

Query: 76  FLRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQIYQHHFLIKAYESG 135
                         AGGF+++D    +K ++T ++  + E LN+G +YQ HFL+++YESG
Sbjct: 62  --------------AGGFESMDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESG 121

Query: 136 VDKNMAMFFIINQLQ--------------------------------------------- 195
            D  M MFF+INQLQ                                             
Sbjct: 122 PDNKMTMFFVINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVD 181

Query: 196 ------------------------------------------------------------ 255
                                                                       
Sbjct: 182 QFPSWNDVVELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCE 241

Query: 256 ---TEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINF 284
               E+ P+FLD  PI+EE+ I+S KL  +        LVTR SDLDMN H+NNASYINF
Sbjct: 242 ELKEEMNPFFLDSHPILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINF 301

BLAST of MS009455 vs. ExPASy Swiss-Prot
Match: G3ESV0 (Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea viscosissima OX=857185 GN=FATB2 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 2.0e-22
Identity = 67/187 (35.83%), Postives = 101/187 (54.01%), Query Frame = 0

Query: 98  RNPKKLDMTIDDSSMGELLNNGQIYQHHFLIKAYESGVDKNMAMFFIINQLQTEITPYFL 157
           +N  + D  I D + GE+L              +     K   +  I ++++ EI P+F+
Sbjct: 222 KNGMRRDWLISDCNTGEILTRAS--------SVWVMMNQKTRKLSKIPDEVRREIEPHFV 281

Query: 158 DCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINFVLESTPRWLFE 217
           D  P+IE++     KLD  S  S    L  R +DLD+N HVNNA YI ++LESTP  + E
Sbjct: 282 DSAPVIEDDDRKLPKLDEKSADSIRKGLTPRWNDLDVNQHVNNAKYIGWILESTPPEVLE 341

Query: 218 NYQLSTVTMKYLRECGMDKQLQSLTVPMKNDINGDDHNKRKDIKFHHSI-LADGIELVRG 277
             +L ++T++Y RECG +  L+SLT     D +G+ +      +F H + L DG E+V+G
Sbjct: 342 TQELCSLTLEYRRECGRESVLESLTAV---DPSGEGYGS----QFQHLLRLEDGGEIVKG 393

Query: 278 RTIWTPK 284
           RT W PK
Sbjct: 402 RTEWRPK 393

BLAST of MS009455 vs. ExPASy Swiss-Prot
Match: Q39513 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana OX=36775 GN=FATB1 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.2e-21
Identity = 65/187 (34.76%), Postives = 99/187 (52.94%), Query Frame = 0

Query: 98  RNPKKLDMTIDDSSMGELLNNGQIYQHHFLIKAYESGVDKNMAMFFIINQLQTEITPYFL 157
           +N  + D  I D + GE+L              +     K   +  I ++++ EI P+F+
Sbjct: 225 KNGMRRDWLISDCNTGEILTRAS--------SVWVMMNQKTRRLSKIPDEVRNEIEPHFV 284

Query: 158 DCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINFVLESTPRWLFE 217
           D  P+IE++     KLD  +  S    L  R +DLD+N HVNN  YI ++LESTP  + E
Sbjct: 285 DSPPVIEDDDRKLPKLDEKTADSIRKGLTPRWNDLDVNQHVNNVKYIGWILESTPPEVLE 344

Query: 218 NYQLSTVTMKYLRECGMDKQLQSLTVPMKNDINGDDHNKRKDIKFHHSI-LADGIELVRG 277
             +L ++T++Y RECG +  L+SLT     D +G  +      +F H + L DG E+V+G
Sbjct: 345 TQELCSLTLEYRRECGRESVLESLTA---MDPSGGGYGS----QFQHLLRLEDGGEIVKG 396

Query: 278 RTIWTPK 284
           RT W PK
Sbjct: 405 RTEWRPK 396

BLAST of MS009455 vs. ExPASy Swiss-Prot
Match: Q9SQI3 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum OX=3635 GN=FATB1 PE=1 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 8.4e-21
Identity = 59/142 (41.55%), Postives = 87/142 (61.27%), Query Frame = 0

Query: 144 IINQLQTEITPYFLDCDPIIEEEKIISNKLDVNSDGSHI-TCLVTRPSDLDMNLHVNNAS 203
           I  +++ EI P+F++ DP++ E+     KLD +S   H+   L  + SDLD+N HVNN  
Sbjct: 259 IPEEVRGEIEPFFMNSDPVLAEDSQKLVKLD-DSTAEHVCKGLTPKWSDLDVNQHVNNVK 318

Query: 204 YINFVLESTPRWLFENYQLSTVTMKYLRECGMDKQLQSLTVPMKNDINGDDHNKRKDIKF 263
           YI ++LES P  + E+++LS +T++Y RECG D  LQSLT    +D N ++     +   
Sbjct: 319 YIGWILESAPLPILESHELSALTLEYRRECGRDSVLQSLTT--VSDSNTENAVNVGEFNC 378

Query: 264 HHSI-LADGIELVRGRTIWTPK 284
            H + L DG E+VRGRT W PK
Sbjct: 379 QHLLRLDDGAEIVRGRTRWRPK 397

BLAST of MS009455 vs. ExPASy Swiss-Prot
Match: Q9SJE2 (Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FATB PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 2.4e-20
Identity = 57/141 (40.43%), Postives = 83/141 (58.87%), Query Frame = 0

Query: 144 IINQLQTEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASY 203
           I  +++ EI PYF++ DP++ E+     K+D  +     + L  R SDLD+N HVNN  Y
Sbjct: 264 IPEEVRGEIEPYFVNSDPVLAEDSRKLTKIDDKTADYVRSGLTPRWSDLDVNQHVNNVKY 323

Query: 204 INFVLESTPRWLFENYQLSTVTMKYLRECGMDKQLQSLTVPMKNDINGDDHNKRKDIKFH 263
           I ++LES P  + E  +L ++T++Y RECG D  LQSLT     DI   +     D++  
Sbjct: 324 IGWILESAPVGIMERQKLKSMTLEYRRECGRDSVLQSLTAVTGCDIG--NLATAGDVECQ 383

Query: 264 HSI-LADGIELVRGRTIWTPK 284
           H + L DG E+VRGRT W+ K
Sbjct: 384 HLLRLQDGAEVVRGRTEWSSK 402

BLAST of MS009455 vs. ExPASy Swiss-Prot
Match: G3ESV1 (Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissima OX=857185 GN=FATB3 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.3e-18
Identity = 61/186 (32.80%), Postives = 94/186 (50.54%), Query Frame = 0

Query: 98  RNPKKLDMTIDDSSMGELLNNGQIYQHHFLIKAYESGVDKNMAMFFIINQLQTEITPYFL 157
           +N    D  I D   GE+L              +     K   +  I ++++ EI PYF+
Sbjct: 222 KNGMSRDWLISDCHSGEILIRA--------TSVWAMMNQKTRRLSKIPDEVRQEIVPYFV 281

Query: 158 DCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINFVLESTPRWLFE 217
           D  P+IE+++ + +KLDV +  S    L  R +D D+N HVNN  YI ++L+S P  +FE
Sbjct: 282 DSAPVIEDDRKL-HKLDVKTGDSIRNGLTPRWNDFDVNQHVNNVKYIAWLLKSVPTEVFE 341

Query: 218 NYQLSTVTMKYLRECGMDKQLQSLTVPMKNDINGDDHNKRKDIKFHHSILADGIELVRGR 277
             +L  +T++Y REC  D  L+S+T  M     GD     + +  H   L +G ++  GR
Sbjct: 342 TQELCGLTLEYRRECRRDSVLESVTA-MDPSKEGD-----RSLYQHLLRLENGADIALGR 392

Query: 278 TIWTPK 284
           T W PK
Sbjct: 402 TEWRPK 392

BLAST of MS009455 vs. ExPASy TrEMBL
Match: A0A5A7UZ85 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002510 PE=3 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 4.1e-55
Identity = 146/386 (37.82%), Postives = 188/386 (48.70%), Query Frame = 0

Query: 2   MAVMSSSNICNFGKEIITISKREQSFGHAIRGNLQLKGHAQYSSLRLKTNIAQITACKMM 61
           MA +SSS  CN GK+  +  K+EQ FG +  GNLQLK H +     L+T           
Sbjct: 1   MAAVSSSYCCNLGKKFNSNLKKEQRFGDSNTGNLQLKNHPKLFLEGLRT----------- 60

Query: 62  MMKNPAFSNFFWGFLRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQI 121
                  SN           I     +A   + I+S   + LD+T  + +MGELLN+G +
Sbjct: 61  -------SN-----------IIAKSSIASEVRKINSYESRNLDITHGNLTMGELLNDGLV 120

Query: 122 YQHHFLIKAYESGVDKNMAMFFIINQL--------------------------------- 181
           YQ HFL+K+YESG DK M MFFIINQL                                 
Sbjct: 121 YQRHFLVKSYESGPDKKMTMFFIINQLHESKLNHLKSCGLVTTGLGTTPEMTKRGLIWVV 180

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 181 HKLQVVVDQYPSWNDIVQLQTWSCFYGKNSYRRDWIVRNYKTGQVLIRASCFYVLMNRNT 240

Query: 242 ----------QTEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHV 283
                     + E+ P+FL  DPIIEEE IIS KLDV++ G + T LVTR SDLDMN HV
Sbjct: 241 RKLSKFYDEMKEEMNPHFLKSDPIIEEENIISTKLDVDAKGCNCTSLVTRLSDLDMNHHV 300

BLAST of MS009455 vs. ExPASy TrEMBL
Match: A0A6J1H8Q2 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461080 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 5.8e-49
Identity = 132/378 (34.92%), Postives = 178/378 (47.09%), Query Frame = 0

Query: 16  EIITISKREQSFGHAIRGNLQLKGHAQYSSLRLK-TNIAQITACKMMMMKNPAFSNFFWG 75
           E+++IS RE +FGHA   +L LKG  + +SL LK ++I+Q T                  
Sbjct: 2   ELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSSHISQPT------------------ 61

Query: 76  FLRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQIYQHHFLIKAYESG 135
                         AGGF+++D    +K ++T ++  + E LN+G +YQ HFL+++YESG
Sbjct: 62  --------------AGGFESMDDYMSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESG 121

Query: 136 VDKNMAMFFIINQLQ--------------------------------------------- 195
            D  M MFF+INQLQ                                             
Sbjct: 122 PDNKMTMFFVINQLQESKLNHFISCGLVERGLGKTGFGTTHEMTKRDLIWVVHKLQVVVD 181

Query: 196 ------------------------------------------------------------ 255
                                                                       
Sbjct: 182 QFPSWNDVVELRTWICKHGKNSYRRNWIARDKKSGKVLIRAICFYVLMNRRTRKLSKFCE 241

Query: 256 ---TEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINF 284
               E+ P+FLD  PI+EE+ I+S KL  +        LVTR SDLDMN H+NNASYINF
Sbjct: 242 ELKEEMNPFFLDSHPILEEDNIVSQKLHFHQHYCTHASLVTRLSDLDMNQHINNASYINF 301

BLAST of MS009455 vs. ExPASy TrEMBL
Match: A0A6J1KZF4 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111497736 PE=3 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 7.6e-49
Identity = 130/377 (34.48%), Postives = 177/377 (46.95%), Query Frame = 0

Query: 16  EIITISKREQSFGHAIRGNLQLKGHAQYSSLRLKTNIAQITACKMMMMKNPAFSNFFWGF 75
           E+++IS RE +FGHA   +L LKG  + +SL LK++           +  P         
Sbjct: 2   ELVSISMREPTFGHATTPSLHLKGRPKSASLTLKSS----------HISQP--------- 61

Query: 76  LRDDLLIFLLFLVAGGFKNIDSRNPKKLDMTIDDSSMGELLNNGQIYQHHFLIKAYESGV 135
                       +A GFK++D    +K ++T ++  + E LN+G +YQ HFL+++YESG 
Sbjct: 62  ------------IASGFKSMDDYVSRKPEITRENLMVEEFLNDGLVYQCHFLVRSYESGP 121

Query: 136 DKNMAMFFIINQLQ---------------------------------------------- 195
           D  M MFF+INQLQ                                              
Sbjct: 122 DNKMTMFFVINQLQESKLNHFISCGLVKTGLGKTGFGTTHEMTKRDLIWVVHKLQVVVDQ 181

Query: 196 ------------------------------------------------------------ 255
                                                                       
Sbjct: 182 FPSWNDVVELRTWICRHGKNSYRRNWIARDKKSGKVLIRAICSYVLMNKRTRKLSKFCEE 241

Query: 256 --TEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINFV 284
              E+ P+FLD  PI+EE+ I+S KL ++        LVTR SDLDMN H+NNASYINFV
Sbjct: 242 LEEEMNPFFLDSHPILEEDNIVSQKLHLHQHDCTYASLVTRLSDLDMNQHINNASYINFV 301

BLAST of MS009455 vs. ExPASy TrEMBL
Match: A0A1S4E345 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo OX=3656 GN=LOC107991799 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 1.3e-40
Identity = 85/137 (62.04%), Postives = 110/137 (80.29%), Query Frame = 0

Query: 148 LQTEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINFV 207
           ++ E+ P+FL  DPIIEEE IIS KLDV++ G + T LVTR SDLDMN HVNNASYINF+
Sbjct: 1   MKEEMNPHFLKSDPIIEEENIISTKLDVDAMGCNCTSLVTRLSDLDMNHHVNNASYINFI 60

Query: 208 LESTPRWLFENYQLSTVTMKYLRECGMDKQLQSLTVPMKNDI--NGDDHNKRKDIKFHHS 267
           LE TPRW+ E+++LSTVT+KYL+ECG+DK LQSL++P++ND+  NG+   K   IK HHS
Sbjct: 61  LEGTPRWMLESHRLSTVTVKYLKECGVDKILQSLSMPIQNDVISNGNSLVKENYIKLHHS 120

Query: 268 ILADGIELVRGRTIWTP 283
           I+++GIELVR  T+W P
Sbjct: 121 IVSEGIELVRATTLWIP 137

BLAST of MS009455 vs. ExPASy TrEMBL
Match: A0A0A0KAB7 (Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_7G440560 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 1.0e-37
Identity = 80/137 (58.39%), Postives = 107/137 (78.10%), Query Frame = 0

Query: 148 LQTEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASYINFV 207
           ++ E+ P+FL  DPIIEE+ IIS KLDV++ G + T LVTR SDLDMN HVNNASYINF+
Sbjct: 1   MKEEMNPHFLHSDPIIEEDNIISTKLDVHAKGCNCTSLVTRLSDLDMNHHVNNASYINFI 60

Query: 208 LESTPRWLFENYQLSTVTMKYLRECGMDKQLQSLTVPMKNDI--NGDDHNKRKDIKFHHS 267
           LE TP W+ E++ LS +T+KYL+ECG+ K+LQSL++P++ND   NG+   +   IK HHS
Sbjct: 61  LEGTPGWVLESHWLSALTIKYLKECGVGKKLQSLSMPIQNDTVSNGNYLVRENFIKLHHS 120

Query: 268 ILADGIELVRGRTIWTP 283
           +L++GIELVR  T+WTP
Sbjct: 121 VLSEGIELVRATTLWTP 137

BLAST of MS009455 vs. TAIR 10
Match: AT1G08510.1 (fatty acyl-ACP thioesterases B )

HSP 1 Score: 100.9 bits (250), Expect = 1.7e-21
Identity = 57/141 (40.43%), Postives = 83/141 (58.87%), Query Frame = 0

Query: 144 IINQLQTEITPYFLDCDPIIEEEKIISNKLDVNSDGSHITCLVTRPSDLDMNLHVNNASY 203
           I  +++ EI PYF++ DP++ E+     K+D  +     + L  R SDLD+N HVNN  Y
Sbjct: 264 IPEEVRGEIEPYFVNSDPVLAEDSRKLTKIDDKTADYVRSGLTPRWSDLDVNQHVNNVKY 323

Query: 204 INFVLESTPRWLFENYQLSTVTMKYLRECGMDKQLQSLTVPMKNDINGDDHNKRKDIKFH 263
           I ++LES P  + E  +L ++T++Y RECG D  LQSLT     DI   +     D++  
Sbjct: 324 IGWILESAPVGIMERQKLKSMTLEYRRECGRDSVLQSLTAVTGCDIG--NLATAGDVECQ 383

Query: 264 HSI-LADGIELVRGRTIWTPK 284
           H + L DG E+VRGRT W+ K
Sbjct: 384 HLLRLQDGAEVVRGRTEWSSK 402

BLAST of MS009455 vs. TAIR 10
Match: AT3G25110.1 (fatA acyl-ACP thioesterase )

HSP 1 Score: 74.7 bits (182), Expect = 1.3e-13
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 0

Query: 185 LVTRPSDLDMNLHVNNASYINFVLESTPRWLFENYQLSTVTMKYLRECGMDKQLQSLTVP 244
           L  R +DLDMN HVNN +YI +VLES P+ + + ++L  +T+ Y REC  D  + SLT  
Sbjct: 254 LKPRRADLDMNQHVNNVTYIGWVLESIPQEIVDTHELQVITLDYRRECQQDDVVDSLTT- 313

Query: 245 MKNDINGDDHNK------RKDIKFHH--SILADGIELVRGRTIWTPKPT 286
             ++I G + +         D +F H   +  DG E+ RG T+W  KP+
Sbjct: 314 TTSEIGGTNGSATSGTQGHNDSQFLHLLRLSGDGQEINRGTTLWRKKPS 361

BLAST of MS009455 vs. TAIR 10
Match: AT4G13050.1 (Acyl-ACP thioesterase )

HSP 1 Score: 69.3 bits (168), Expect = 5.6e-12
Identity = 47/149 (31.54%), Postives = 70/149 (46.98%), Query Frame = 0

Query: 155 YFLDCDP-----IIEEEKIISNKLDVNSDGSHITCLVTRP--SDLDMNLHVNNASYINFV 214
           Y + C P       EE      K+    D +  + L  +P  +DLDMN HVNN +YI +V
Sbjct: 216 YLVFCPPEPRLAFPEENNSSLKKIPKLEDPAQYSMLGLKPRRADLDMNQHVNNVTYIGWV 275

Query: 215 LESTPRWLFENYQLSTVTMKYLRECGMDKQLQSLT--------VPMKNDINGDDHNKRKD 274
           LES P+ + + ++L  +T+ Y REC  D  + SLT        V      NG   + +++
Sbjct: 276 LESIPQEIIDTHELKVITLDYRRECQQDDIVDSLTTSETPNEVVSKLTGTNGSTTSSKRE 335

Query: 275 IKFHH-----SILADGIELVRGRTIWTPK 284
               H      +  +G E+ RGRT W  K
Sbjct: 336 HNESHFLHILRLSENGQEINRGRTQWRKK 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0058801.18.6e-5537.82palmitoyl-acyl carrier protein thioesterase [Cucumis melo var. makuwa] >TYK10596... [more]
KAG6593401.11.2e-5336.06Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita a... [more]
KAG7025748.11.2e-5344.52Palmitoyl-acyl carrier protein thioesterase, chloroplastic, partial [Cucurbita a... [more]
XP_023514527.11.6e-5335.81palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita pepo ... [more]
XP_022960310.11.2e-4834.92palmitoyl-acyl carrier protein thioesterase, chloroplastic-like [Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
G3ESV02.0e-2235.83Acyl-[acyl-carrier-protein] hydrolase FATB2, chloroplastic (Fragment) OS=Cuphea ... [more]
Q395132.2e-2134.76Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Cuphea hookeriana ... [more]
Q9SQI38.4e-2141.55Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Gossypium hirsutum... [more]
Q9SJE22.4e-2040.43Palmitoyl-acyl carrier protein thioesterase, chloroplastic OS=Arabidopsis thalia... [more]
G3ESV12.3e-1832.80Acyl-[acyl-carrier-protein] hydrolase FATB3, chloroplastic OS=Cuphea viscosissim... [more]
Match NameE-valueIdentityDescription
A0A5A7UZ854.1e-5537.82Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1H8Q25.8e-4934.92Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461... [more]
A0A6J1KZF47.6e-4934.48Acyl-[acyl-carrier-protein] hydrolase OS=Cucurbita maxima OX=3661 GN=LOC11149773... [more]
A0A1S4E3451.3e-4062.04Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis melo OX=3656 GN=LOC107991799 PE... [more]
A0A0A0KAB71.0e-3758.39Acyl-[acyl-carrier-protein] hydrolase OS=Cucumis sativus OX=3659 GN=Csa_7G440560... [more]
Match NameE-valueIdentityDescription
AT1G08510.11.7e-2140.43fatty acyl-ACP thioesterases B [more]
AT3G25110.11.3e-1338.53fatA acyl-ACP thioesterase [more]
AT4G13050.15.6e-1231.54Acyl-ACP thioesterase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002864Acyl-ACP thioesterasePFAMPF01643Acyl-ACP_TEcoord: 173..281
e-value: 1.0E-20
score: 74.1
NoneNo IPR availableGENE3D3.10.129.10Hotdog Thioesterasecoord: 150..285
e-value: 1.8E-27
score: 98.1
NoneNo IPR availablePANTHERPTHR31727:SF2PALMITOYL-ACYL CARRIER PROTEIN THIOESTERASE, CHLOROPLASTICcoord: 146..284
NoneNo IPR availablePANTHERPTHR31727:SF2PALMITOYL-ACYL CARRIER PROTEIN THIOESTERASE, CHLOROPLASTICcoord: 50..149
IPR045023Acyl-[acyl-carrier-protein] hydrolase FATA/BPANTHERPTHR31727OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTICcoord: 50..149
IPR045023Acyl-[acyl-carrier-protein] hydrolase FATA/BPANTHERPTHR31727OLEOYL-ACYL CARRIER PROTEIN THIOESTERASE 1, CHLOROPLASTICcoord: 146..284
IPR029069HotDog domain superfamilySUPERFAMILY54637Thioesterase/thiol ester dehydrase-isomerasecoord: 172..281

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009455.1MS009455.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
molecular_function GO:0016297 acyl-[acyl-carrier-protein] hydrolase activity
molecular_function GO:0016790 thiolester hydrolase activity