MS009447 (gene) Bitter gourd (TR) v1

Overview


Name	MS009447
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Gibberellin 3-beta-dioxygenase 2
Location	scaffold813: 1348508 .. 1348933 (-)
RNA-Seq Expression	MS009447
Synteny	MS009447

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGTTGAACTCGTACCCGGTATGTCCAGATCCAGATCGAGCCATGGGTCTCGGGGTCCACAGCGACACCAGCCTCCTCACGGTGGTGTACCAAAATAATACTAAAGGGTTGCAGGTGTTACGGGAAGGGAACCGGTGGGTGACGGTAGAGGCGGTACCTGATAGGCTGGTGGTCCAGGTGGGAGACCTGCTCCACATTATGACAAACGGGTTGTACCCTGCTGTGCTCCACCAAGCGGTCGTGAACCGAACGCGGAAACGACAATCCGTTGTGTACGTATTTGGGCCACCAGAAAGCGCTGAAATCTCACCACTCAAGAAGCTTTTGGGTCCAACCCAGCCCCCTCTCTACCCCGCAATCACTTGGTCTGAATACCTCGGAACCAAGGCGAAGCATTTCAACGACACACTTTCTTTCATCCGCCTA

mRNA sequence

CAGTTGAACTCGTACCCGGTATGTCCAGATCCAGATCGAGCCATGGGTCTCGGGGTCCACAGCGACACCAGCCTCCTCACGGTGGTGTACCAAAATAATACTAAAGGGTTGCAGGTGTTACGGGAAGGGAACCGGTGGGTGACGGTAGAGGCGGTACCTGATAGGCTGGTGGTCCAGGTGGGAGACCTGCTCCACATTATGACAAACGGGTTGTACCCTGCTGTGCTCCACCAAGCGGTCGTGAACCGAACGCGGAAACGACAATCCGTTGTGTACGTATTTGGGCCACCAGAAAGCGCTGAAATCTCACCACTCAAGAAGCTTTTGGGTCCAACCCAGCCCCCTCTCTACCCCGCAATCACTTGGTCTGAATACCTCGGAACCAAGGCGAAGCATTTCAACGACACACTTTCTTTCATCCGCCTA

Coding sequence (CDS)

CAGTTGAACTCGTACCCGGTATGTCCAGATCCAGATCGAGCCATGGGTCTCGGGGTCCACAGCGACACCAGCCTCCTCACGGTGGTGTACCAAAATAATACTAAAGGGTTGCAGGTGTTACGGGAAGGGAACCGGTGGGTGACGGTAGAGGCGGTACCTGATAGGCTGGTGGTCCAGGTGGGAGACCTGCTCCACATTATGACAAACGGGTTGTACCCTGCTGTGCTCCACCAAGCGGTCGTGAACCGAACGCGGAAACGACAATCCGTTGTGTACGTATTTGGGCCACCAGAAAGCGCTGAAATCTCACCACTCAAGAAGCTTTTGGGTCCAACCCAGCCCCCTCTCTACCCCGCAATCACTTGGTCTGAATACCTCGGAACCAAGGCGAAGCATTTCAACGACACACTTTCTTTCATCCGCCTA

Protein sequence

QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQVGDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAITWSEYLGTKAKHFNDTLSFIRL

Homology

[Close]

BLAST of MS009447 vs. NCBI nr
Match: XP_022146386.1 (gibberellin 3-beta-dioxygenase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 292.7 bits (748), Expect = 1.7e-75
Identity = 141/142 (99.30%), Postives = 142/142 (100.00%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLNSYPVCP+PDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV
Sbjct: 213 QLNSYPVCPNPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 272

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI
Sbjct: 273 GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 332

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TWSEYLGTKAKHFNDTLSFIRL
Sbjct: 333 TWSEYLGTKAKHFNDTLSFIRL 354

[Close]

BLAST of MS009447 vs. NCBI nr
Match: XP_022146378.1 (gibberellin 3-beta-dioxygenase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 292.7 bits (748), Expect = 1.7e-75
Identity = 141/142 (99.30%), Postives = 142/142 (100.00%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLNSYPVCP+PDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV
Sbjct: 213 QLNSYPVCPNPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 272

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI
Sbjct: 273 GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 332

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TWSEYLGTKAKHFNDTLSFIRL
Sbjct: 333 TWSEYLGTKAKHFNDTLSFIRL 354

[Close]

BLAST of MS009447 vs. NCBI nr
Match: XP_022145559.1 (gibberellin 3-beta-dioxygenase 1-like [Momordica charantia])

HSP 1 Score: 261.5 bits (667), Expect = 4.1e-66
Identity = 123/142 (86.62%), Postives = 130/142 (91.55%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           Q NSYP+CPDPDRAMGLGVH+DTSLLTVVYQNNT+GLQVLREG RWVTVE VP  LVV V
Sbjct: 265 QFNSYPICPDPDRAMGLGVHTDTSLLTVVYQNNTRGLQVLREGKRWVTVEPVPSGLVVHV 324

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHI+TNGLYP+VLHQAVVNRTRKR SV YVFGPPESAEISPLKKLLGPTQPPLY  +
Sbjct: 325 GDLLHILTNGLYPSVLHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPV 384

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TWSEYLGTKAKHFND LS +RL
Sbjct: 385 TWSEYLGTKAKHFNDALSSVRL 406

[Close]

BLAST of MS009447 vs. NCBI nr
Match: XP_004135830.1 (gibberellin 3-beta-dioxygenase 1 [Cucumis sativus] >KGN45321.1 hypothetical protein Csa_015611 [Cucumis sativus] >CBX88045.1 gibberellin 3-oxidase, partial [Cucumis sativus])

HSP 1 Score: 252.7 bits (644), Expect = 1.9e-63
Identity = 118/142 (83.10%), Postives = 129/142 (90.85%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLNSYPVCPDPDRAMGLG H+DTSLLT+VYQNNT+GLQVLREGNRWVTVE VP  LVVQV
Sbjct: 214 QLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQNNTRGLQVLREGNRWVTVEPVPGALVVQV 273

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHI+TNGLYP+ +HQAVVNRTRKR SV YVFGPPESAEISPLKKLLGPTQPPLY  +
Sbjct: 274 GDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTV 333

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TW+EYL  KA+HFN+ LS +RL
Sbjct: 334 TWTEYLRKKAEHFNNALSSVRL 355

[Close]

BLAST of MS009447 vs. NCBI nr
Match: XP_038898776.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 252.7 bits (644), Expect = 1.9e-63
Identity = 118/142 (83.10%), Postives = 129/142 (90.85%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLNSYPVCPDPDRAMGLG H+DTSLLT+VYQNNT+GLQVLREGNRWVTVE VP  LVVQV
Sbjct: 215 QLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQNNTRGLQVLREGNRWVTVEPVPGALVVQV 274

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHI+TNGLYP+ +HQAVVNRTRKR SV YVFGPPESAEISPLKKLLGPTQPPLY  +
Sbjct: 275 GDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTV 334

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TW+EYL  KA+HFN+ LS +RL
Sbjct: 335 TWTEYLRKKAEHFNNALSSVRL 356

[Close]

BLAST of MS009447 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 2.5e-50
Identity = 89/142 (62.68%), Postives = 113/142 (79.58%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLNSYP CPDPD AMGL  H+D++ LT++ QN+  GLQV REG+ W+TV  +   LVV V
Sbjct: 212 QLNSYPSCPDPDHAMGLAPHTDSTFLTILSQNDISGLQVNREGSGWITVPPLQGGLVVNV 271

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDL HI++NGLYP+VLH+ +VNRTR+R SV Y++GPP + EI P  KL+GPT+PPLY ++
Sbjct: 272 GDLFHILSNGLYPSVLHRVLVNRTRQRFSVAYLYGPPSNVEICPHAKLIGPTKPPLYRSV 331

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TW+EYLGTKAKHFN  LS +RL
Sbjct: 332 TWNEYLGTKAKHFNKALSSVRL 353

[Close]

BLAST of MS009447 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 4.0e-48
Identity = 86/141 (60.99%), Postives = 107/141 (75.89%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLN YP CP+PDRAMGL  H+D++L+T++YQNNT GLQV R+   WVT   VP  LVV V
Sbjct: 206 QLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNV 265

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHI+TNG++P+VLH+A VN  R R S+ Y++GPP    ISPL KL+ P Q PLYP++
Sbjct: 266 GDLLHILTNGIFPSVLHRARVNHVRSRFSMAYLWGPPSDIMISPLPKLVDPLQSPLYPSL 325

Query: 121 TWSEYLGTKAKHFNDTLSFIR 142
           TW +YL TKA HFN +LS IR
Sbjct: 326 TWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of MS009447 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 184.9 bits (468), Expect = 6.4e-46
Identity = 84/141 (59.57%), Postives = 106/141 (75.18%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLN YPVCP+PDRAMGL  H+D++LLT++YQNNT GLQV R+   WVTV   P  LVV V
Sbjct: 213 QLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNV 272

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDL HI++NGL+ +VLH+A VN+TR R SV +++GP    +ISP+ KL+ P + PLY ++
Sbjct: 273 GDLFHILSNGLFKSVLHRARVNQTRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSV 332

Query: 121 TWSEYLGTKAKHFNDTLSFIR 142
           TW EYL TKA HFN  LS IR
Sbjct: 333 TWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of MS009447 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-39
Identity = 74/143 (51.75%), Postives = 104/143 (72.73%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           +LN YPVCP+P+RAMGL  H+D+++LT+++Q+NT GLQV RE + WVTVE  P  LVV +
Sbjct: 208 RLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNI 267

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVF-GPPESAEISPLKKLLGPTQPPLYPA 120
           GDL HI++NG  P+V+H+A VN TR R S+ Y++ GP    +I+P+ KL GP +P LY +
Sbjct: 268 GDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRS 327

Query: 121 ITWSEYLGTKAKHFNDTLSFIRL 143
           ITW EYL  K + F+  +  IR+
Sbjct: 328 ITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of MS009447 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.0e-35
Identity = 71/144 (49.31%), Postives = 94/144 (65.28%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQV---LREGNRWVTVEAVPDRLV 60
           QLNSYPVCPDP  AMGL  H+D+SLLT++YQ N  GL++     EG+RW+ VE +   LV
Sbjct: 207 QLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLV 266

Query: 61  VQVGDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLY 120
           V +GDL HI++NG + + +H+AVVN+T  R S  Y  GPP++ +I PL        PP+Y
Sbjct: 267 VIMGDLSHIISNGQFRSTMHRAVVNKTHHRVSAAYFAGPPKNLQIGPLTS--DKNHPPIY 326

Query: 121 PAITWSEYLGTKAKHFNDTLSFIR 142
             + W EYL  KA HFN  L+  R
Sbjct: 327 RRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of MS009447 vs. ExPASy TrEMBL
Match: A0A6J1CYF2 (gibberellin 3-beta-dioxygenase 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015603 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 8.1e-76
Identity = 141/142 (99.30%), Postives = 142/142 (100.00%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLNSYPVCP+PDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV
Sbjct: 213 QLNSYPVCPNPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 272

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI
Sbjct: 273 GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 332

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TWSEYLGTKAKHFNDTLSFIRL
Sbjct: 333 TWSEYLGTKAKHFNDTLSFIRL 354

[Close]

BLAST of MS009447 vs. ExPASy TrEMBL
Match: A0A6J1CZ75 (gibberellin 3-beta-dioxygenase 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015603 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 8.1e-76
Identity = 141/142 (99.30%), Postives = 142/142 (100.00%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLNSYPVCP+PDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV
Sbjct: 213 QLNSYPVCPNPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 272

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI
Sbjct: 273 GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 332

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TWSEYLGTKAKHFNDTLSFIRL
Sbjct: 333 TWSEYLGTKAKHFNDTLSFIRL 354

[Close]

BLAST of MS009447 vs. ExPASy TrEMBL
Match: A0A6J1CVL5 (gibberellin 3-beta-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111014979 PE=3 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.0e-66
Identity = 123/142 (86.62%), Postives = 130/142 (91.55%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           Q NSYP+CPDPDRAMGLGVH+DTSLLTVVYQNNT+GLQVLREG RWVTVE VP  LVV V
Sbjct: 265 QFNSYPICPDPDRAMGLGVHTDTSLLTVVYQNNTRGLQVLREGKRWVTVEPVPSGLVVHV 324

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHI+TNGLYP+VLHQAVVNRTRKR SV YVFGPPESAEISPLKKLLGPTQPPLY  +
Sbjct: 325 GDLLHILTNGLYPSVLHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPV 384

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TWSEYLGTKAKHFND LS +RL
Sbjct: 385 TWSEYLGTKAKHFNDALSSVRL 406

[Close]

BLAST of MS009447 vs. ExPASy TrEMBL
Match: K7ZTU0 (Gibberellin 3-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga3ox1 PE=2 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 9.2e-64
Identity = 118/142 (83.10%), Postives = 129/142 (90.85%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLNSYPVCPDPDRAMGLG H+DTSLLT+VYQNNT+GLQVLREGNRWVTVE VP  LVVQV
Sbjct: 214 QLNSYPVCPDPDRAMGLGAHTDTSLLTIVYQNNTRGLQVLREGNRWVTVEPVPGALVVQV 273

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHI+TNGLYP+ +HQAVVNRTRKR SV YVFGPPESAEISPLKKLLGPTQPPLY  +
Sbjct: 274 GDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTV 333

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TW+EYL  KA+HFN+ LS +RL
Sbjct: 334 TWTEYLRKKAEHFNNALSSVRL 355

[Close]

BLAST of MS009447 vs. ExPASy TrEMBL
Match: A0A5A7US98 (Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002410 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 7.8e-63
Identity = 116/142 (81.69%), Postives = 128/142 (90.14%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           Q NSYPVCPDP+RAMGLG H+DTSLLT+VYQNNT+GLQVLREGNRWVTVE VP  LVVQV
Sbjct: 214 QFNSYPVCPDPERAMGLGAHTDTSLLTIVYQNNTRGLQVLREGNRWVTVEPVPGALVVQV 273

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHI+TNGLYP+ +HQAVVNRTRKR SV YVFGPPESAEISPLKKLLGPTQPPLY  +
Sbjct: 274 GDLLHILTNGLYPSPVHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRTV 333

Query: 121 TWSEYLGTKAKHFNDTLSFIRL 143
           TW+EYL  KA+HFN+ LS +RL
Sbjct: 334 TWTEYLRKKAEHFNNALSSVRL 355

[Close]

BLAST of MS009447 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 192.2 bits (487), Expect = 2.9e-49
Identity = 86/141 (60.99%), Postives = 107/141 (75.89%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLN YP CP+PDRAMGL  H+D++L+T++YQNNT GLQV R+   WVT   VP  LVV V
Sbjct: 206 QLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNV 265

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDLLHI+TNG++P+VLH+A VN  R R S+ Y++GPP    ISPL KL+ P Q PLYP++
Sbjct: 266 GDLLHILTNGIFPSVLHRARVNHVRSRFSMAYLWGPPSDIMISPLPKLVDPLQSPLYPSL 325

Query: 121 TWSEYLGTKAKHFNDTLSFIR 142
           TW +YL TKA HFN +LS IR
Sbjct: 326 TWKQYLATKATHFNQSLSIIR 346

[Close]

BLAST of MS009447 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 184.9 bits (468), Expect = 4.6e-47
Identity = 84/141 (59.57%), Postives = 106/141 (75.18%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           QLN YPVCP+PDRAMGL  H+D++LLT++YQNNT GLQV R+   WVTV   P  LVV V
Sbjct: 213 QLNHYPVCPEPDRAMGLAAHTDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNV 272

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPAI 120
           GDL HI++NGL+ +VLH+A VN+TR R SV +++GP    +ISP+ KL+ P + PLY ++
Sbjct: 273 GDLFHILSNGLFKSVLHRARVNQTRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSV 332

Query: 121 TWSEYLGTKAKHFNDTLSFIR 142
           TW EYL TKA HFN  LS IR
Sbjct: 333 TWKEYLRTKATHFNKALSMIR 353

[Close]

BLAST of MS009447 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 163.7 bits (413), Expect = 1.1e-40
Identity = 74/143 (51.75%), Postives = 104/143 (72.73%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQVLREGNRWVTVEAVPDRLVVQV 60
           +LN YPVCP+P+RAMGL  H+D+++LT+++Q+NT GLQV RE + WVTVE  P  LVV +
Sbjct: 208 RLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWVTVEPAPGVLVVNI 267

Query: 61  GDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVF-GPPESAEISPLKKLLGPTQPPLYPA 120
           GDL HI++NG  P+V+H+A VN TR R S+ Y++ GP    +I+P+ KL GP +P LY +
Sbjct: 268 GDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPISKLTGPAEPSLYRS 327

Query: 121 ITWSEYLGTKAKHFNDTLSFIRL 143
           ITW EYL  K + F+  +  IR+
Sbjct: 328 ITWKEYLQIKYEVFDKAMDAIRV 350

[Close]

BLAST of MS009447 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 151.0 bits (380), Expect = 7.3e-37
Identity = 71/144 (49.31%), Postives = 94/144 (65.28%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQNNTKGLQV---LREGNRWVTVEAVPDRLV 60
           QLNSYPVCPDP  AMGL  H+D+SLLT++YQ N  GL++     EG+RW+ VE +   LV
Sbjct: 207 QLNSYPVCPDPHLAMGLAPHTDSSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLV 266

Query: 61  VQVGDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLY 120
           V +GDL HI++NG + + +H+AVVN+T  R S  Y  GPP++ +I PL        PP+Y
Sbjct: 267 VIMGDLSHIISNGQFRSTMHRAVVNKTHHRVSAAYFAGPPKNLQIGPLTS--DKNHPPIY 326

Query: 121 PAITWSEYLGTKAKHFNDTLSFIR 142
             + W EYL  KA HFN  L+  R
Sbjct: 327 RRLIWEEYLAAKATHFNKALTLFR 348

[Close]

BLAST of MS009447 vs. TAIR 10
Match: AT4G25310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 2.1e-23
Identity = 52/126 (41.27%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 1   QLNSYPVCPDPDRAMGLGVHSDTSLLTVVYQ-NNTKGLQVLREGNRWVTVEAVPDRLVVQ 60
           ++N YP CP+PD+A+GL  HSD + LT++ Q N  +GLQ+ ++G +WV+V+ +P+ LVV 
Sbjct: 209 RMNYYPPCPEPDKAIGLTPHSDATGLTILLQVNEVEGLQIKKDG-KWVSVKPLPNALVVN 268

Query: 61  VGDLLHIMTNGLYPAVLHQAVVNRTRKRQSVVYVFGPPESAEISPLKKLLGPTQPPLYPA 120
           VGD+L I+TNG Y ++ H+ VVN  ++R SV          EI P++ L+   +  L+  
Sbjct: 269 VGDILEIITNGTYRSIEHRGVVNSEKERLSVASFHNTGFGKEIGPMRSLVERHKGALFKT 328

Query: 121 ITWSEY 126
           +T  EY
Sbjct: 329 LTTEEY 333

The following BLAST results are available for this feature:

BLAST of MS009447 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146386.1	1.7e-75	99.30	gibberellin 3-beta-dioxygenase 2 isoform X2 [Momordica charantia]	[more]
XP_022146378.1	1.7e-75	99.30	gibberellin 3-beta-dioxygenase 2 isoform X1 [Momordica charantia]	[more]
XP_022145559.1	4.1e-66	86.62	gibberellin 3-beta-dioxygenase 1-like [Momordica charantia]	[more]
XP_004135830.1	1.9e-63	83.10	gibberellin 3-beta-dioxygenase 1 [Cucumis sativus] >KGN45321.1 hypothetical prot...	[more]
XP_038898776.1	1.9e-63	83.10	gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida]	[more]

BLAST of MS009447 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24648	2.5e-50	62.68	Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1	[more]
Q9ZT84	4.0e-48	60.99	Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ...	[more]
Q39103	6.4e-46	59.57	Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ...	[more]
Q9C971	1.5e-39	51.75	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9SVS8	1.0e-35	49.31	Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ...	[more]

BLAST of MS009447 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CYF2	8.1e-76	99.30	gibberellin 3-beta-dioxygenase 2 isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1CZ75	8.1e-76	99.30	gibberellin 3-beta-dioxygenase 2 isoform X2 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1CVL5	2.0e-66	86.62	gibberellin 3-beta-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
K7ZTU0	9.2e-64	83.10	Gibberellin 3-oxidase (Fragment) OS=Cucumis sativus OX=3659 GN=ga3ox1 PE=2 SV=1	[more]
A0A5A7US98	7.8e-63	81.69	Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of MS009447 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80340.1	2.9e-49	60.99	gibberellin 3-oxidase 2	[more]
AT1G15550.1	4.6e-47	59.57	gibberellin 3-oxidase 1	[more]
AT1G80330.1	1.1e-40	51.75	gibberellin 3-oxidase 4	[more]
AT4G21690.1	7.3e-37	49.31	gibberellin 3-oxidase 3	[more]
AT4G25310.1	2.1e-23	41.27	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 20..41 score: 47.11 coord: 56..82 score: 26.6
None	No IPR available	PANTHER	PTHR47991:SF87	GIBBERELLIN 3-BETA-DIOXYGENASE 1-LIKE	coord: 1..133
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 1..133
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 2..133
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 2..95 e-value: 3.8E-23 score: 81.9
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..127 e-value: 4.3E-44 score: 153.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 1..96 score: 11.466463

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009447.1	MS009447.1	mRNA