MS009446 (gene) Bitter gourd (TR) v1

BLAST of MS009446 vs. NCBI nr
Match: XP_022145559.1 (gibberellin 3-beta-dioxygenase 1-like [Momordica charantia])

HSP 1 Score: 772.7 bits (1994), Expect = 1.4e-219
Identity = 370/373 (99.20%), Postives = 371/373 (99.46%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLI 60
           MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFP LADTDSDFDTVPLI
Sbjct: 53  MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPSLADTDSDFDTVPLI 112

Query: 61  DLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG 120
           DLSL NVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG
Sbjct: 113 DLSLSNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG 172

Query: 121 VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR 180
           VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR
Sbjct: 173 VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR 232

Query: 181 LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV 240
           LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV
Sbjct: 233 LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV 292

Query: 241 VYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL 300
           VYQNNTRGLQVL+EGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL
Sbjct: 293 VYQNNTRGLQVLREGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL 352

Query: 301 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS 360
           SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS
Sbjct: 353 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS 412

Query: 361 LLDINDHSSVTVG 374
           LLDINDHSSVTVG
Sbjct: 413 LLDINDHSSVTVG 425

BLAST of MS009446 vs. NCBI nr
Match: XP_022146378.1 (gibberellin 3-beta-dioxygenase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 638.3 bits (1645), Expect = 4.2e-179
Identity = 300/372 (80.65%), Postives = 328/372 (88.17%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLI 60
           MATT K+TDAFKSHPVH+PVHKNLDFDS QELPDSYAW+QPA  P +AD DS+ DTVPL+
Sbjct: 1   MATTTKLTDAFKSHPVHVPVHKNLDFDSSQELPDSYAWVQPANLPLIADPDSNSDTVPLV 60

Query: 61  DLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG 120
           DLSLPN AH VA ACSTWGAFQVINHGI +SLLDR++SAANSLFSLP   KLKAARSPDG
Sbjct: 61  DLSLPNAAHLVATACSTWGAFQVINHGIPSSLLDRIDSAANSLFSLPPDKKLKAARSPDG 120

Query: 121 VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR 180
           VTGYG++ ISSFFPKRMWSEGFTI GSPLDHFRQ WPNDYT+YCD + EYEREMKSLC R
Sbjct: 121 VTGYGILHISSFFPKRMWSEGFTIAGSPLDHFRQFWPNDYTKYCDMIEEYEREMKSLCKR 180

Query: 181 LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV 240
           LIW+ALGELGIT ED+KWA  NGDFQ +HSV Q NSYP+CP+PDRAMGLGVH+DTSLLTV
Sbjct: 181 LIWLALGELGITWEDVKWADLNGDFQGNHSVVQLNSYPVCPNPDRAMGLGVHSDTSLLTV 240

Query: 241 VYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL 300
           VYQNNT+GLQVL+EG RWVTVE VP  LVV VGDLLHI+TNGLYP+VLHQAVVNRTRKR 
Sbjct: 241 VYQNNTKGLQVLREGNRWVTVEAVPDRLVVQVGDLLHIMTNGLYPAVLHQAVVNRTRKRQ 300

Query: 301 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS 360
           SV YVFGPPESAEISPLKKLLGPTQPPLY  +TWSEYLGTKAKHFND LS +RL AP   
Sbjct: 301 SVVYVFGPPESAEISPLKKLLGPTQPPLYPAITWSEYLGTKAKHFNDTLSFIRLPAPFIG 360

Query: 361 LLDINDHSSVTV 373
             D+ND++SV V
Sbjct: 361 QSDVNDNNSVIV 372

BLAST of MS009446 vs. NCBI nr
Match: XP_022146386.1 (gibberellin 3-beta-dioxygenase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 634.8 bits (1636), Expect = 4.7e-178
Identity = 296/372 (79.57%), Postives = 326/372 (87.63%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLI 60
           MATT K+TDAFKS P+H+P HKNLDFDS QELPDSYAW+QPA  PP+ D DS+ DTVPL+
Sbjct: 1   MATTTKLTDAFKSRPIHVPAHKNLDFDSSQELPDSYAWVQPANLPPVVDLDSNSDTVPLV 60

Query: 61  DLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG 120
           DLSLPN AH +A ACSTWGAFQVINHGI +SLLDR++SAANSLFSLP   KLKAARSPDG
Sbjct: 61  DLSLPNAAHLIATACSTWGAFQVINHGIPSSLLDRIDSAANSLFSLPPDKKLKAARSPDG 120

Query: 121 VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR 180
           VTGYG++ ISSFFPKRMWSEGFTI GSPLDHFRQ WPNDYT+YCD + EYEREMKSLC R
Sbjct: 121 VTGYGILHISSFFPKRMWSEGFTIAGSPLDHFRQFWPNDYTKYCDMIEEYEREMKSLCKR 180

Query: 181 LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV 240
           LIW+ALGELGIT ED+KWA  NGDFQ +HSV Q NSYP+CP+PDRAMGLGVH+DTSLLTV
Sbjct: 181 LIWLALGELGITWEDVKWADLNGDFQGNHSVVQLNSYPVCPNPDRAMGLGVHSDTSLLTV 240

Query: 241 VYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL 300
           VYQNNT+GLQVL+EG RWVTVE VP  LVV VGDLLHI+TNGLYP+VLHQAVVNRTRKR 
Sbjct: 241 VYQNNTKGLQVLREGNRWVTVEAVPDRLVVQVGDLLHIMTNGLYPAVLHQAVVNRTRKRQ 300

Query: 301 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS 360
           SV YVFGPPESAEISPLKKLLGPTQPPLY  +TWSEYLGTKAKHFND LS +RL AP   
Sbjct: 301 SVVYVFGPPESAEISPLKKLLGPTQPPLYPAITWSEYLGTKAKHFNDTLSFIRLPAPFIG 360

Query: 361 LLDINDHSSVTV 373
             D+ND++SV V
Sbjct: 361 QSDVNDNNSVIV 372

BLAST of MS009446 vs. NCBI nr
Match: XP_008461056.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 624.8 bits (1610), Expect = 4.9e-175
Identity = 291/374 (77.81%), Postives = 327/374 (87.43%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDF-DTVPL 60
           M +  KIT+ FKSHPVHIP+HKN DFDS QELPDSY WIQP +FP  + +++   D++PL
Sbjct: 1   MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL 60

Query: 61  IDLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPD 120
           IDLSLPN  H + NA  TWGAFQVINHG+  SLL  +ES+ANSLFSLP  HKLKAAR  D
Sbjct: 61  IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD 120

Query: 121 GVTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCG 180
           G++GYGLVRISSFFPKRMWSEGFTIVGSPL+HFR+LWP+DYT YCD + EY++EMKSLCG
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCG 180

Query: 181 RLIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLT 240
           RL+W+ALGELGITRED+ WAGPNGDF  S++ TQFNSYP+CPDP+RAMGLG HTDTSLLT
Sbjct: 181 RLMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLT 240

Query: 241 VVYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKR 300
           +VYQNNTRGLQVL+EG RWVTVEPVP  LVV VGDLLHILTNGLYPS +HQAVVNRTRKR
Sbjct: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300

Query: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLT 360
           LSVAYVFGPPESAEISPLKKLLGPTQPPLYR VTW+EYL  KA+HFN+ALSSVRLCAPLT
Sbjct: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360

Query: 361 SLLDINDHSSVTVG 374
            LLD+NDHS V VG
Sbjct: 361 GLLDVNDHSQVKVG 374

BLAST of MS009446 vs. NCBI nr
Match: XP_022959819.1 (gibberellin 3-beta-dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 624.8 bits (1610), Expect = 4.9e-175
Identity = 295/377 (78.25%), Postives = 331/377 (87.80%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPP----LADTDSDFDT 60
           MATT  I D FKS PVHIP HKNLDFDS  ELPDSYAWIQP +FP      +  DSD D+
Sbjct: 1   MATT--IADVFKSFPVHIPAHKNLDFDSLHELPDSYAWIQPDSFPTHKHHNSILDSDADS 60

Query: 61  VPLIDLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAAR 120
           VPLIDLSLPN A  + NA  +WGAFQVINHG+  SLL  +ES+A++LFSLP  HKLKAAR
Sbjct: 61  VPLIDLSLPNAATLIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAAR 120

Query: 121 SPDGVTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKS 180
           +PDG++GYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWP+DY ++C+ + EY+REM+S
Sbjct: 121 TPDGISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMRS 180

Query: 181 LCGRLIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTS 240
           LCGRL+W+ LGELGITR+DMKWAGP+GDF+ S + TQFNSYP+CPDPDRAMGLG HTDTS
Sbjct: 181 LCGRLMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDTS 240

Query: 241 LLTVVYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRT 300
           LLT+VYQNNTRGLQVL+EGKRWVTVEPV  GLVV VGDLLHILTNGLYPS LHQAVVNRT
Sbjct: 241 LLTIVYQNNTRGLQVLREGKRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNRT 300

Query: 301 RKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCA 360
           RKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTW+EYLG KA+HFN+ALS+VR+CA
Sbjct: 301 RKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKKAEHFNNALSTVRVCA 360

Query: 361 PLTSLLDINDHSSVTVG 374
           P+T LLD+NDHS V VG
Sbjct: 361 PITGLLDVNDHSRVKVG 375

BLAST of MS009446 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 2.1e-128
Identity = 217/373 (58.18%), Postives = 276/373 (73.99%), Query Frame = 0

Query: 7   ITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDS----DFDTVPLIDL 66
           +++A+++HPVH+  HK+ DF+S QELP+SY W        L D+++       TVP+IDL
Sbjct: 4   LSEAYRAHPVHVN-HKHPDFNSLQELPESYNWTH-LDDHTLIDSNNIMKESTTTVPVIDL 63

Query: 67  SLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVT 126
           + PN +  +  AC TWG +QV+NHGI  SLL+ ++    +LFSLP H K KA RSPDGV+
Sbjct: 64  NDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSPDGVS 123

Query: 127 GYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGRLI 186
           GYG+ RISSFFPK MW EGFTIVGSPLDHFR+LWP DYT +CD + +Y+  MK L G L+
Sbjct: 124 GYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLAGTLM 183

Query: 187 WMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTVVY 246
            + L  LGIT+ED+KWAG    F+ + +  Q NSYP CPDPD AMGL  HTD++ LT++ 
Sbjct: 184 CLMLDSLGITKEDIKWAGSKAQFEKACAALQLNSYPSCPDPDHAMGLAPHTDSTFLTILS 243

Query: 247 QNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRLSV 306
           QN+  GLQV +EG  W+TV P+  GLVV+VGDL HIL+NGLYPSVLH+ +VNRTR+R SV
Sbjct: 244 QNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVNRTRQRFSV 303

Query: 307 AYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTSLL 366
           AY++GPP + EI P  KL+GPT+PPLYR VTW+EYLGTKAKHFN ALSSVRLC P+  L 
Sbjct: 304 AYLYGPPSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTKAKHFNKALSSVRLCTPINGLF 363

Query: 367 DIND--HSSVTVG 374
           D+ND   +SV VG
Sbjct: 364 DVNDSNKNSVQVG 374

BLAST of MS009446 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 422.2 bits (1084), Expect = 6.3e-117
Identity = 193/347 (55.62%), Postives = 251/347 (72.33%), Query Frame = 0

Query: 7   ITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLIDLSLPN 66
           ++D F+SHP+HIP+    DF S   LPDSY W        L    +  +T+PLIDLS  +
Sbjct: 5   LSDVFRSHPIHIPLSNPPDFKS---LPDSYTWTPKDDL--LFSASASDETLPLIDLSDIH 64

Query: 67  VAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVTGYGL 126
           VA  V +AC+TWGAFQ+ NHG+ + LLD +E    SLF LP+  KLKAARS +GV+GYG+
Sbjct: 65  VATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGV 124

Query: 127 VRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWMAL 186
            RI+SFF K+MWSEGFT++GSPL  FR+LWP+ + +YC+ + EYE  M+ L  +L+W AL
Sbjct: 125 ARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFAL 184

Query: 187 GELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTVVYQNNT 246
           G LG+  +D++WAGPN DFQ + +V Q N YP CP+PDRAMGL  HTD++L+T++YQNNT
Sbjct: 185 GSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNT 244

Query: 247 RGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRLSVAYVF 306
            GLQV ++   WVT  PVP  LVV+VGDLLHILTNG++PSVLH+A VN  R R S+AY++
Sbjct: 245 AGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYLW 304

Query: 307 GPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVR 354
           GPP    ISPL KL+ P Q PLY  +TW +YL TKA HFN +LS +R
Sbjct: 305 GPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of MS009446 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 409.1 bits (1050), Expect = 5.5e-113
Identity = 185/349 (53.01%), Postives = 242/349 (69.34%), Query Frame = 0

Query: 7   ITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWI--QPATFPPLADTDSDFDTVPLIDLSL 66
           +TD F+ HP+H+P     DF S +ELPDSY W       F       +  + +PLIDL  
Sbjct: 5   LTDVFRGHPIHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDH 64

Query: 67  PNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVTGY 126
           P+  + + +AC TWGAFQ+ NHG+   LL  +E    SLF LP+  KLK+ARS  GV+GY
Sbjct: 65  PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGY 124

Query: 127 GLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWM 186
           G+ RI+SFF K+MWSEGFTI GSPL+ FR+LWP  +  YCD + EYE  MK L  +L+W+
Sbjct: 125 GVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWL 184

Query: 187 ALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTVVYQN 246
           AL  LG++ ED++WA  + D   + +  Q N YP+CP+PDRAMGL  HTD++LLT++YQN
Sbjct: 185 ALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQN 244

Query: 247 NTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRLSVAY 306
           NT GLQV ++   WVTV P P  LVV+VGDL HIL+NGL+ SVLH+A VN+TR RLSVA+
Sbjct: 245 NTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLSVAF 304

Query: 307 VFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVR 354
           ++GP    +ISP+ KL+ P + PLY+ VTW EYL TKA HFN ALS +R
Sbjct: 305 LWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of MS009446 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 9.1e-92
Identity = 163/339 (48.08%), Postives = 227/339 (66.96%), Query Frame = 0

Query: 28  SFQELPDSYAWIQPATFPPLADT-----DSDFDTVPLIDLSLPNVAHHVANACSTWGAFQ 87
           S Q LP+S+ W   A    L  +     D+  +++P+IDLS P+V   + +A  TWGAFQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 88  VINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVTGYGLVRISSFFPKRMWSEGF 147
           + NHGIS  LLD +ES + +LF +P   KL+AA S  GV+GYG  RIS FF K+MWSEGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 148 TIV-GSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWMALGELGITREDMKWAG- 207
           TI   S  +HF  LWP+D+T+YC  ++EY  EM+ L  RL++  LG LG+T ED++WA  
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194

Query: 208 -PNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTVVYQNNTRGLQVLQEGKRWV 267
                 +      + N YP+CP+P+RAMGL  HTD+++LT+++Q+NT GLQV +E   WV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254

Query: 268 TVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRLSVAYVF-GPPESAEISPLK 327
           TVEP P  LVV++GDL HIL+NG  PSV+H+A VN TR R+S+AY++ GP    +I+P+ 
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314

Query: 328 KLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAP 358
           KL GP +P LYR +TW EYL  K + F+ A+ ++R+  P
Sbjct: 315 KLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRVVNP 353

BLAST of MS009446 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.2e-88
Identity = 161/354 (45.48%), Postives = 222/354 (62.71%), Query Frame = 0

Query: 7   ITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLIDLSLPN 66
           +T  FK++PV+      LDF + + LPDS+ W +P       + ++    +P+I LS P 
Sbjct: 4   VTQLFKNNPVNRDRIIPLDFTNTKTLPDSHVWSKP-------EPETTSGPIPVISLSNPE 63

Query: 67  VAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVTGYGL 126
               +  AC  WG F + +HG+S SLL  ++     LFSLP+H K+ A RSPD  TGYG+
Sbjct: 64  EHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGV 123

Query: 127 VRISSFFPKRMWSEGFTIVGSPL-DHFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWMA 186
           VRIS F+ K MWSEGF+++GS L  H   LWP+D+ E+C+ M EY++ M  L  RLI M 
Sbjct: 124 VRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISML 183

Query: 187 LGELGITREDMKWAGPNGDFQASHSVTQF---NSYPICPDPDRAMGLGVHTDTSLLTVVY 246
           +G LG+T ED+ W  P+     + S+  F   NSYP+CPDP  AMGL  HTD+SLLT++Y
Sbjct: 184 MGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILY 243

Query: 247 QNNTRGLQV---LQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKR 306
           Q N  GL++    +EG RW+ VEP+   LVV +GDL HI++NG + S +H+AVVN+T  R
Sbjct: 244 QGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHHR 303

Query: 307 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVR 354
           +S AY  GPP++ +I PL        PP+YR + W EYL  KA HFN AL+  R
Sbjct: 304 VSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

BLAST of MS009446 vs. ExPASy TrEMBL
Match: A0A6J1CVL5 (gibberellin 3-beta-dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111014979 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 7.0e-220
Identity = 370/373 (99.20%), Postives = 371/373 (99.46%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLI 60
           MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFP LADTDSDFDTVPLI
Sbjct: 53  MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPSLADTDSDFDTVPLI 112

Query: 61  DLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG 120
           DLSL NVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG
Sbjct: 113 DLSLSNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG 172

Query: 121 VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR 180
           VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR
Sbjct: 173 VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR 232

Query: 181 LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV 240
           LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV
Sbjct: 233 LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV 292

Query: 241 VYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL 300
           VYQNNTRGLQVL+EGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL
Sbjct: 293 VYQNNTRGLQVLREGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL 352

Query: 301 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS 360
           SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS
Sbjct: 353 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS 412

Query: 361 LLDINDHSSVTVG 374
           LLDINDHSSVTVG
Sbjct: 413 LLDINDHSSVTVG 425

BLAST of MS009446 vs. ExPASy TrEMBL
Match: A0A6J1CYF2 (gibberellin 3-beta-dioxygenase 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015603 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.1e-179
Identity = 300/372 (80.65%), Postives = 328/372 (88.17%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLI 60
           MATT K+TDAFKSHPVH+PVHKNLDFDS QELPDSYAW+QPA  P +AD DS+ DTVPL+
Sbjct: 1   MATTTKLTDAFKSHPVHVPVHKNLDFDSSQELPDSYAWVQPANLPLIADPDSNSDTVPLV 60

Query: 61  DLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG 120
           DLSLPN AH VA ACSTWGAFQVINHGI +SLLDR++SAANSLFSLP   KLKAARSPDG
Sbjct: 61  DLSLPNAAHLVATACSTWGAFQVINHGIPSSLLDRIDSAANSLFSLPPDKKLKAARSPDG 120

Query: 121 VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR 180
           VTGYG++ ISSFFPKRMWSEGFTI GSPLDHFRQ WPNDYT+YCD + EYEREMKSLC R
Sbjct: 121 VTGYGILHISSFFPKRMWSEGFTIAGSPLDHFRQFWPNDYTKYCDMIEEYEREMKSLCKR 180

Query: 181 LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV 240
           LIW+ALGELGIT ED+KWA  NGDFQ +HSV Q NSYP+CP+PDRAMGLGVH+DTSLLTV
Sbjct: 181 LIWLALGELGITWEDVKWADLNGDFQGNHSVVQLNSYPVCPNPDRAMGLGVHSDTSLLTV 240

Query: 241 VYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL 300
           VYQNNT+GLQVL+EG RWVTVE VP  LVV VGDLLHI+TNGLYP+VLHQAVVNRTRKR 
Sbjct: 241 VYQNNTKGLQVLREGNRWVTVEAVPDRLVVQVGDLLHIMTNGLYPAVLHQAVVNRTRKRQ 300

Query: 301 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS 360
           SV YVFGPPESAEISPLKKLLGPTQPPLY  +TWSEYLGTKAKHFND LS +RL AP   
Sbjct: 301 SVVYVFGPPESAEISPLKKLLGPTQPPLYPAITWSEYLGTKAKHFNDTLSFIRLPAPFIG 360

Query: 361 LLDINDHSSVTV 373
             D+ND++SV V
Sbjct: 361 QSDVNDNNSVIV 372

BLAST of MS009446 vs. ExPASy TrEMBL
Match: A0A6J1CZ75 (gibberellin 3-beta-dioxygenase 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015603 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.3e-178
Identity = 296/372 (79.57%), Postives = 326/372 (87.63%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLI 60
           MATT K+TDAFKS P+H+P HKNLDFDS QELPDSYAW+QPA  PP+ D DS+ DTVPL+
Sbjct: 1   MATTTKLTDAFKSRPIHVPAHKNLDFDSSQELPDSYAWVQPANLPPVVDLDSNSDTVPLV 60

Query: 61  DLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDG 120
           DLSLPN AH +A ACSTWGAFQVINHGI +SLLDR++SAANSLFSLP   KLKAARSPDG
Sbjct: 61  DLSLPNAAHLIATACSTWGAFQVINHGIPSSLLDRIDSAANSLFSLPPDKKLKAARSPDG 120

Query: 121 VTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGR 180
           VTGYG++ ISSFFPKRMWSEGFTI GSPLDHFRQ WPNDYT+YCD + EYEREMKSLC R
Sbjct: 121 VTGYGILHISSFFPKRMWSEGFTIAGSPLDHFRQFWPNDYTKYCDMIEEYEREMKSLCKR 180

Query: 181 LIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTV 240
           LIW+ALGELGIT ED+KWA  NGDFQ +HSV Q NSYP+CP+PDRAMGLGVH+DTSLLTV
Sbjct: 181 LIWLALGELGITWEDVKWADLNGDFQGNHSVVQLNSYPVCPNPDRAMGLGVHSDTSLLTV 240

Query: 241 VYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRL 300
           VYQNNT+GLQVL+EG RWVTVE VP  LVV VGDLLHI+TNGLYP+VLHQAVVNRTRKR 
Sbjct: 241 VYQNNTKGLQVLREGNRWVTVEAVPDRLVVQVGDLLHIMTNGLYPAVLHQAVVNRTRKRQ 300

Query: 301 SVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLTS 360
           SV YVFGPPESAEISPLKKLLGPTQPPLY  +TWSEYLGTKAKHFND LS +RL AP   
Sbjct: 301 SVVYVFGPPESAEISPLKKLLGPTQPPLYPAITWSEYLGTKAKHFNDTLSFIRLPAPFIG 360

Query: 361 LLDINDHSSVTV 373
             D+ND++SV V
Sbjct: 361 QSDVNDNNSVIV 372

BLAST of MS009446 vs. ExPASy TrEMBL
Match: A0A6J1H974 (gibberellin 3-beta-dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460769 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.3e-175
Identity = 295/377 (78.25%), Postives = 331/377 (87.80%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPP----LADTDSDFDT 60
           MATT  I D FKS PVHIP HKNLDFDS  ELPDSYAWIQP +FP      +  DSD D+
Sbjct: 1   MATT--IADVFKSFPVHIPAHKNLDFDSLHELPDSYAWIQPDSFPTHKHHNSILDSDADS 60

Query: 61  VPLIDLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAAR 120
           VPLIDLSLPN A  + NA  +WGAFQVINHG+  SLL  +ES+A++LFSLP  HKLKAAR
Sbjct: 61  VPLIDLSLPNAATLIGNAFRSWGAFQVINHGVPISLLQSIESSADTLFSLPPSHKLKAAR 120

Query: 121 SPDGVTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKS 180
           +PDG++GYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWP+DY ++C+ + EY+REM+S
Sbjct: 121 TPDGISGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPHDYHKHCEIVEEYDREMRS 180

Query: 181 LCGRLIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTS 240
           LCGRL+W+ LGELGITR+DMKWAGP+GDF+ S + TQFNSYP+CPDPDRAMGLG HTDTS
Sbjct: 181 LCGRLMWLGLGELGITRDDMKWAGPDGDFKTSPAATQFNSYPVCPDPDRAMGLGPHTDTS 240

Query: 241 LLTVVYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRT 300
           LLT+VYQNNTRGLQVL+EGKRWVTVEPV  GLVV VGDLLHILTNGLYPS LHQAVVNRT
Sbjct: 241 LLTIVYQNNTRGLQVLREGKRWVTVEPVAGGLVVQVGDLLHILTNGLYPSALHQAVVNRT 300

Query: 301 RKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCA 360
           RKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTW+EYLG KA+HFN+ALS+VR+CA
Sbjct: 301 RKRLSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWTEYLGKKAEHFNNALSTVRVCA 360

Query: 361 PLTSLLDINDHSSVTVG 374
           P+T LLD+NDHS V VG
Sbjct: 361 PITGLLDVNDHSRVKVG 375

BLAST of MS009446 vs. ExPASy TrEMBL
Match: A0A1S3CDD3 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103499758 PE=3 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.3e-175
Identity = 291/374 (77.81%), Postives = 327/374 (87.43%), Query Frame = 0

Query: 1   MATTKKITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDF-DTVPL 60
           M +  KIT+ FKSHPVHIP+HKN DFDS QELPDSY WIQP +FP  + +++   D++PL
Sbjct: 1   MGSNIKITEVFKSHPVHIPIHKNFDFDSLQELPDSYDWIQPDSFPNSSLSNNHLSDSIPL 60

Query: 61  IDLSLPNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPD 120
           IDLSLPN  H + NA  TWGAFQVINHG+  SLL  +ES+ANSLFSLP  HKLKAAR  D
Sbjct: 61  IDLSLPNAPHLIGNAFRTWGAFQVINHGVPISLLHSIESSANSLFSLPAPHKLKAARPSD 120

Query: 121 GVTGYGLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCG 180
           G++GYGLVRISSFFPKRMWSEGFTIVGSPL+HFR+LWP+DYT YCD + EY++EMKSLCG
Sbjct: 121 GISGYGLVRISSFFPKRMWSEGFTIVGSPLEHFRKLWPHDYTRYCDIVEEYDQEMKSLCG 180

Query: 181 RLIWMALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLT 240
           RL+W+ALGELGITRED+ WAGPNGDF  S++ TQFNSYP+CPDP+RAMGLG HTDTSLLT
Sbjct: 181 RLMWVALGELGITREDVNWAGPNGDFMTSNAATQFNSYPVCPDPERAMGLGAHTDTSLLT 240

Query: 241 VVYQNNTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKR 300
           +VYQNNTRGLQVL+EG RWVTVEPVP  LVV VGDLLHILTNGLYPS +HQAVVNRTRKR
Sbjct: 241 IVYQNNTRGLQVLREGNRWVTVEPVPGALVVQVGDLLHILTNGLYPSPVHQAVVNRTRKR 300

Query: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAPLT 360
           LSVAYVFGPPESAEISPLKKLLGPTQPPLYR VTW+EYL  KA+HFN+ALSSVRLCAPLT
Sbjct: 301 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRTVTWTEYLRKKAEHFNNALSSVRLCAPLT 360

Query: 361 SLLDINDHSSVTVG 374
            LLD+NDHS V VG
Sbjct: 361 GLLDVNDHSQVKVG 374

BLAST of MS009446 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 422.2 bits (1084), Expect = 4.5e-118
Identity = 193/347 (55.62%), Postives = 251/347 (72.33%), Query Frame = 0

Query: 7   ITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLIDLSLPN 66
           ++D F+SHP+HIP+    DF S   LPDSY W        L    +  +T+PLIDLS  +
Sbjct: 5   LSDVFRSHPIHIPLSNPPDFKS---LPDSYTWTPKDDL--LFSASASDETLPLIDLSDIH 64

Query: 67  VAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVTGYGL 126
           VA  V +AC+TWGAFQ+ NHG+ + LLD +E    SLF LP+  KLKAARS +GV+GYG+
Sbjct: 65  VATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSENGVSGYGV 124

Query: 127 VRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWMAL 186
            RI+SFF K+MWSEGFT++GSPL  FR+LWP+ + +YC+ + EYE  M+ L  +L+W AL
Sbjct: 125 ARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAAKLMWFAL 184

Query: 187 GELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTVVYQNNT 246
           G LG+  +D++WAGPN DFQ + +V Q N YP CP+PDRAMGL  HTD++L+T++YQNNT
Sbjct: 185 GSLGVEEKDIQWAGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTLMTILYQNNT 244

Query: 247 RGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRLSVAYVF 306
            GLQV ++   WVT  PVP  LVV+VGDLLHILTNG++PSVLH+A VN  R R S+AY++
Sbjct: 245 AGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVRSRFSMAYLW 304

Query: 307 GPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVR 354
           GPP    ISPL KL+ P Q PLY  +TW +YL TKA HFN +LS +R
Sbjct: 305 GPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of MS009446 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 409.1 bits (1050), Expect = 3.9e-114
Identity = 185/349 (53.01%), Postives = 242/349 (69.34%), Query Frame = 0

Query: 7   ITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWI--QPATFPPLADTDSDFDTVPLIDLSL 66
           +TD F+ HP+H+P     DF S +ELPDSY W       F       +  + +PLIDL  
Sbjct: 5   LTDVFRGHPIHLPHSHIPDFTSLRELPDSYKWTPKDDLLFSAAPSPPATGENIPLIDLDH 64

Query: 67  PNVAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVTGY 126
           P+  + + +AC TWGAFQ+ NHG+   LL  +E    SLF LP+  KLK+ARS  GV+GY
Sbjct: 65  PDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKSARSETGVSGY 124

Query: 127 GLVRISSFFPKRMWSEGFTIVGSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWM 186
           G+ RI+SFF K+MWSEGFTI GSPL+ FR+LWP  +  YCD + EYE  MK L  +L+W+
Sbjct: 125 GVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHMKKLASKLMWL 184

Query: 187 ALGELGITREDMKWAGPNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTVVYQN 246
           AL  LG++ ED++WA  + D   + +  Q N YP+CP+PDRAMGL  HTD++LLT++YQN
Sbjct: 185 ALNSLGVSEEDIEWASLSSDLNWAQAALQLNHYPVCPEPDRAMGLAAHTDSTLLTILYQN 244

Query: 247 NTRGLQVLQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRLSVAY 306
           NT GLQV ++   WVTV P P  LVV+VGDL HIL+NGL+ SVLH+A VN+TR RLSVA+
Sbjct: 245 NTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRARVNQTRARLSVAF 304

Query: 307 VFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVR 354
           ++GP    +ISP+ KL+ P + PLY+ VTW EYL TKA HFN ALS +R
Sbjct: 305 LWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMIR 353

BLAST of MS009446 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 338.6 bits (867), Expect = 6.5e-93
Identity = 163/339 (48.08%), Postives = 227/339 (66.96%), Query Frame = 0

Query: 28  SFQELPDSYAWIQPATFPPLADT-----DSDFDTVPLIDLSLPNVAHHVANACSTWGAFQ 87
           S Q LP+S+ W   A    L  +     D+  +++P+IDLS P+V   + +A  TWGAFQ
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAVSFDAVEESIPVIDLSNPDVTTLIGDASKTWGAFQ 74

Query: 88  VINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVTGYGLVRISSFFPKRMWSEGF 147
           + NHGIS  LLD +ES + +LF +P   KL+AA S  GV+GYG  RIS FF K+MWSEGF
Sbjct: 75  IANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSEGF 134

Query: 148 TIV-GSPLDHFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWMALGELGITREDMKWAG- 207
           TI   S  +HF  LWP+D+T+YC  ++EY  EM+ L  RL++  LG LG+T ED++WA  
Sbjct: 135 TIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHK 194

Query: 208 -PNGDFQASHSVTQFNSYPICPDPDRAMGLGVHTDTSLLTVVYQNNTRGLQVLQEGKRWV 267
                 +      + N YP+CP+P+RAMGL  HTD+++LT+++Q+NT GLQV +E   WV
Sbjct: 195 LEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESGWV 254

Query: 268 TVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKRLSVAYVF-GPPESAEISPLK 327
           TVEP P  LVV++GDL HIL+NG  PSV+H+A VN TR R+S+AY++ GP    +I+P+ 
Sbjct: 255 TVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAPIS 314

Query: 328 KLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVRLCAP 358
           KL GP +P LYR +TW EYL  K + F+ A+ ++R+  P
Sbjct: 315 KLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRVVNP 353

BLAST of MS009446 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 328.2 bits (840), Expect = 8.7e-90
Identity = 161/354 (45.48%), Postives = 222/354 (62.71%), Query Frame = 0

Query: 7   ITDAFKSHPVHIPVHKNLDFDSFQELPDSYAWIQPATFPPLADTDSDFDTVPLIDLSLPN 66
           +T  FK++PV+      LDF + + LPDS+ W +P       + ++    +P+I LS P 
Sbjct: 4   VTQLFKNNPVNRDRIIPLDFTNTKTLPDSHVWSKP-------EPETTSGPIPVISLSNPE 63

Query: 67  VAHHVANACSTWGAFQVINHGISTSLLDRLESAANSLFSLPLHHKLKAARSPDGVTGYGL 126
               +  AC  WG F + +HG+S SLL  ++     LFSLP+H K+ A RSPD  TGYG+
Sbjct: 64  EHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSPDESTGYGV 123

Query: 127 VRISSFFPKRMWSEGFTIVGSPL-DHFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWMA 186
           VRIS F+ K MWSEGF+++GS L  H   LWP+D+ E+C+ M EY++ M  L  RLI M 
Sbjct: 124 VRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDLSHRLISML 183

Query: 187 LGELGITREDMKWAGPNGDFQASHSVTQF---NSYPICPDPDRAMGLGVHTDTSLLTVVY 246
           +G LG+T ED+ W  P+     + S+  F   NSYP+CPDP  AMGL  HTD+SLLT++Y
Sbjct: 184 MGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTDSSLLTILY 243

Query: 247 QNNTRGLQV---LQEGKRWVTVEPVPSGLVVHVGDLLHILTNGLYPSVLHQAVVNRTRKR 306
           Q N  GL++    +EG RW+ VEP+   LVV +GDL HI++NG + S +H+AVVN+T  R
Sbjct: 244 QGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRAVVNKTHHR 303

Query: 307 LSVAYVFGPPESAEISPLKKLLGPTQPPLYRPVTWSEYLGTKAKHFNDALSSVR 354
           +S AY  GPP++ +I PL        PP+YR + W EYL  KA HFN AL+  R
Sbjct: 304 VSAAYFAGPPKNLQIGPLTS--DKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

BLAST of MS009446 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.7e-45
Identity = 104/308 (33.77%), Postives = 163/308 (52.92%), Query Frame = 0

Query: 38  WIQPATFPPLAD-TDSDFD-TVPLIDLS----LPNVAHHVANACSTWGAFQVINHGISTS 97
           +++PA   P+ + T SD    +P++D++     P     V +AC  WG FQ++NHG++ S
Sbjct: 27  YVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLRLVRSACEEWGFFQMVNHGVTHS 86

Query: 98  LLDRLESAANSLFSLPLHHKLKAARSPDGVTGYGLVRISSFFPKRMWSEGFTIVGSPLD- 157
           L++R+  A    F LPL  K K A SPD   GYG         K  WS+ F +   P   
Sbjct: 87  LMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSI 146

Query: 158 HFRQLWPNDYTEYCDTMREYEREMKSLCGRLIWMALGELGITREDMKWAGPNGDFQASHS 217
                WP+   +  + + +Y  E++ LC RL       LG+    +  A   GD   +  
Sbjct: 147 RNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSESLGLKPNKLMQALGGGDKVGASL 206

Query: 218 VTQFNSYPICPDPDRAMGLGVHTDTSLLTVVY-QNNTRGLQVLQEGKRWVTVEPVPSGLV 277
            T F  YP CP P   +GL  H+D   +T++       GLQV + G  WVT++ VP+ L+
Sbjct: 207 RTNF--YPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGLQV-RRGDGWVTIKSVPNALI 266

Query: 278 VHVGDLLHILTNGLYPSVLHQAVVNRTRKRLSVAYVFGPPESAEISPLKKLLGPTQPPLY 337
           V++GD L IL+NG+Y SV HQ +VN   +R+S+A+ + P     + P+++L+   +P LY
Sbjct: 267 VNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRPALY 326