MS009445 (gene) Bitter gourd (TR) v1

Overview


Name	MS009445
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold813: 1325736 .. 1327559 (+)
RNA-Seq Expression	MS009445
Synteny	MS009445

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTACGCAAGCCAAATCTAAGATCCCGACAATCGAGTTATCGGAAGAAAAATTGAAAGTAGGAAGCGAAGAATGGGTGTTGATTGGTAAAGAGATACGAGTTGCTTTGGAAGAATTTGGTTGCTTTTTGGTGGTTTATGAAAAAGTTTCTTTGGAGCTCCATAACTCCATTTTTGAAGCAACAAAAGAGCTCTTCCATCTCCCAATTGAAACTAGGATCCAAAATACGAGTGAAAAGCCCTACCATGGCTATTTTGGGCGATACTCCTTTCTTCCTCTCTATGAATCCATGGCCGTGGATAATCCAACTTCTCTGCAAGCAATTCAAACTTTCACCAATCTCATGTGGCCTGCTGGAAACAAAACCTTCTGGTATTTTTTTCAATTTGGTTTTGAATCTACGAATATAGTTGCTATAGTTTGAATACTTGTCTTACGTTACAATATTTATGTTGCTAAGAAACCTAGAGTTCTTAAATCCTGATCAGAAGGTGGCCACTTACGTCCTACGGTTTGAACCTATTTCGTCCCAACCCTTTTAATTTTTCATTAGTCCCAATGGTTAAAAACAATTAAATTTGTTGTAACCCATTGTCTTAAATTTTTGGATTATTTGATACCAATAGTCATTAAGTCCTATTTGGTAACGATCCTGTTTCTTCATTTTTAATAAAGAAAAATGAATTGTACGGCTCCAACATCTATGAACTAAAGTATGATACTACACTATTCTTATTTATGTTTTGGAGCAAATGGAGAATGGGAATACGAAAAAAGAAACTTGTTTTGGTTGTTTCCAAAATTTCTATACAATTTTGGAAATGTTCCCAAAAAAAATAAGAAACAAGAAATGTAGCTAGTTGCAAGATGCTTAGATTTCTTAAAGAAAATTGGAAACGAGAAACAGGAAATAGAAATGTTACCAAACGAGCCCTAAGTTAACCTATGATGGTTCATCTTTCCTTAACTTTTACCCAATCCATTGACTAACACAAAACCAATCAAATAAAAACATAAGTACCAAAATGACTAAATTGAAACTTTTTCAAAACACACCAGCCAAATTGGAATTTGGAACTATGTCCAAAACTACAGCGATCAAGTTTTATTAGTGGAGAAAGTAAGTATTCATGCCAATTTAAACCTAGTTCAATTAGTTAAGACTTGTATCTTCAGTCAAAAGGTGAGAGTTCTCGACGTACACCCTGCATGTTATTGAACTAAAAAACGTGGGTATTTTAGTGAAACAGTTCACGATTTCTCAACGTTGGTGGCGAAACTAGAGGAGACAGTTACAAGAATGGTGTTGGAAAGCTATGGAGTATCGAAGCAAGTGTGTGAGTCGAGCATTGAATCCACGACCCACCTTCTTCGGTGTTTCAAATACAGAGTGCCGAAGCAAGACGAAACCGATCTAGGTTTACACTCCCACACGGATCTCACATTTCTTTCCATACTTCATCAGCATCTCATTGGCGGCTTGCAAGTTAAATCACTTGACAACAATGACGACGAAGAATGGATCGATATTGACCCTGCCGGCCGCTCCTCGTTCATCGTCATGGCCGGCGATGCACTCATGGCATGGAGCAACGACAGGATTAGAGGCTGCAGGCACCGAGTGATGATGAGGGGGGATGAAACCAGATATTCACTTGGCCTATTTTCGTTCAAGAGTGGCACCATTAGTGTGCCTGAGGAGCTGGTTGAGGACAACCATCCCTTGAAATACAAGCCCTTTGATCACTTCGAGTTCCTTCGCTATGACAAAGCAAATGCTTCCCATAAAACTTGCTCTCGTATCAAAGACTATTGTGGCATA

mRNA sequence

ATGGGTACGCAAGCCAAATCTAAGATCCCGACAATCGAGTTATCGGAAGAAAAATTGAAAGTAGGAAGCGAAGAATGGGTGTTGATTGGTAAAGAGATACGAGTTGCTTTGGAAGAATTTGGTTGCTTTTTGGTGGTTTATGAAAAAGTTTCTTTGGAGCTCCATAACTCCATTTTTGAAGCAACAAAAGAGCTCTTCCATCTCCCAATTGAAACTAGGATCCAAAATACGAGTGAAAAGCCCTACCATGGCTATTTTGGGCGATACTCCTTTCTTCCTCTCTATGAATCCATGGCCGTGGATAATCCAACTTCTCTGCAAGCAATTCAAACTTTCACCAATCTCATGTGGCCTGCTGGAAACAAAACCTTCTGTGAAACAGTTCACGATTTCTCAACGTTGGTGGCGAAACTAGAGGAGACAGTTACAAGAATGGTGTTGGAAAGCTATGGAGTATCGAAGCAAGTGTGTGAGTCGAGCATTGAATCCACGACCCACCTTCTTCGGTGTTTCAAATACAGAGTGCCGAAGCAAGACGAAACCGATCTAGGTTTACACTCCCACACGGATCTCACATTTCTTTCCATACTTCATCAGCATCTCATTGGCGGCTTGCAAGTTAAATCACTTGACAACAATGACGACGAAGAATGGATCGATATTGACCCTGCCGGCCGCTCCTCGTTCATCGTCATGGCCGGCGATGCACTCATGGCATGGAGCAACGACAGGATTAGAGGCTGCAGGCACCGAGTGATGATGAGGGGGGATGAAACCAGATATTCACTTGGCCTATTTTCGTTCAAGAGTGGCACCATTAGTGTGCCTGAGGAGCTGGTTGAGGACAACCATCCCTTGAAATACAAGCCCTTTGATCACTTCGAGTTCCTTCGCTATGACAAAGCAAATGCTTCCCATAAAACTTGCTCTCGTATCAAAGACTATTGTGGCATA

Coding sequence (CDS)

ATGGGTACGCAAGCCAAATCTAAGATCCCGACAATCGAGTTATCGGAAGAAAAATTGAAAGTAGGAAGCGAAGAATGGGTGTTGATTGGTAAAGAGATACGAGTTGCTTTGGAAGAATTTGGTTGCTTTTTGGTGGTTTATGAAAAAGTTTCTTTGGAGCTCCATAACTCCATTTTTGAAGCAACAAAAGAGCTCTTCCATCTCCCAATTGAAACTAGGATCCAAAATACGAGTGAAAAGCCCTACCATGGCTATTTTGGGCGATACTCCTTTCTTCCTCTCTATGAATCCATGGCCGTGGATAATCCAACTTCTCTGCAAGCAATTCAAACTTTCACCAATCTCATGTGGCCTGCTGGAAACAAAACCTTCTGTGAAACAGTTCACGATTTCTCAACGTTGGTGGCGAAACTAGAGGAGACAGTTACAAGAATGGTGTTGGAAAGCTATGGAGTATCGAAGCAAGTGTGTGAGTCGAGCATTGAATCCACGACCCACCTTCTTCGGTGTTTCAAATACAGAGTGCCGAAGCAAGACGAAACCGATCTAGGTTTACACTCCCACACGGATCTCACATTTCTTTCCATACTTCATCAGCATCTCATTGGCGGCTTGCAAGTTAAATCACTTGACAACAATGACGACGAAGAATGGATCGATATTGACCCTGCCGGCCGCTCCTCGTTCATCGTCATGGCCGGCGATGCACTCATGGCATGGAGCAACGACAGGATTAGAGGCTGCAGGCACCGAGTGATGATGAGGGGGGATGAAACCAGATATTCACTTGGCCTATTTTCGTTCAAGAGTGGCACCATTAGTGTGCCTGAGGAGCTGGTTGAGGACAACCATCCCTTGAAATACAAGCCCTTTGATCACTTCGAGTTCCTTCGCTATGACAAAGCAAATGCTTCCCATAAAACTTGCTCTCGTATCAAAGACTATTGTGGCATA

Protein sequence

MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFEATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAGNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYDKANASHKTCSRIKDYCGI

Homology

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BLAST of MS009445 vs. NCBI nr
Match: XP_022145687.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Momordica charantia])

HSP 1 Score: 649.0 bits (1673), Expect = 2.0e-182
Identity = 315/318 (99.06%), Postives = 316/318 (99.37%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60
           MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE
Sbjct: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60

Query: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAG 120
           ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAG
Sbjct: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAG 120

Query: 121 NKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDE 180
           NKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDE
Sbjct: 121 NKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDE 180

Query: 181 TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAW 240
           TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNN+D EWIDIDP GRSSFIVMAGDALMAW
Sbjct: 181 TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALMAW 240

Query: 241 SNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYD 300
           SNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYD
Sbjct: 241 SNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYD 300

Query: 301 KANASHKTCSRIKDYCGI 319
           KANASHKTCSRIKDYCGI
Sbjct: 301 KANASHKTCSRIKDYCGI 318

[Close]

BLAST of MS009445 vs. NCBI nr
Match: XP_038898684.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida])

HSP 1 Score: 466.5 bits (1199), Expect = 1.9e-127
Identity = 227/321 (70.72%), Postives = 272/321 (84.74%), Query Frame = 0


Query: 1   MGTQAKSKI-PTIELSEEKLKV-GSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSI 60
           + ++AK+K+ PTI+LS  KL+  G+EEW L  KE+R+ LEEFGCF VVY++VSLELHNSI
Sbjct: 2   VNSEAKAKVLPTIDLS--KLEAGGTEEWGLACKEMRLGLEEFGCFSVVYDRVSLELHNSI 61

Query: 61  FEATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP 120
           FEATKELFHLP+ETRI+NTSEKPYHGYFG YS LPLYESM +DNP  LQA Q+F NLMWP
Sbjct: 62  FEATKELFHLPLETRIKNTSEKPYHGYFGGYSVLPLYESMGIDNPIELQATQSFANLMWP 121

Query: 121 A-GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPK 180
              N  FC TV  FSTLVA+LE+ VT+MVLESYG+SK V E ++ES  +LLRCFKYR P+
Sbjct: 122 TKENNHFCGTVERFSTLVAELEKMVTKMVLESYGLSKLVNEPNMESANYLLRCFKYRAPE 181

Query: 181 QDETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDAL 240
           +DETD+GLHSHTDLTF SI+HQHLI GLQV+SL    D++WIDI P+G   FIVMAGDAL
Sbjct: 182 KDETDVGLHSHTDLTFFSIIHQHLISGLQVQSL----DDQWIDIQPSGHCYFIVMAGDAL 241

Query: 241 MAWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFL 300
           MAWSN+RIR CRHRV+MRG+ETRYS+G+FSFK+GTI+VPEELV+D +PL+YKPF+H++FL
Sbjct: 242 MAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIAVPEELVDDANPLRYKPFNHYDFL 301

Query: 301 RYDKANASHKTCSRIKDYCGI 319
            YDKA ASH+  SRIKDYCGI
Sbjct: 302 TYDKAKASHQNVSRIKDYCGI 316

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BLAST of MS009445 vs. NCBI nr
Match: XP_008461057.1 (PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumis melo])

HSP 1 Score: 465.3 bits (1196), Expect = 4.2e-127
Identity = 227/321 (70.72%), Postives = 270/321 (84.11%), Query Frame = 0


Query: 1   MGTQAKSK--IPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSI 60
           M  +AKSK  IPTI+LS  KL+VG++EW L  KE+R  LEEFGCF VVY+KVSLELHNSI
Sbjct: 1   MVCEAKSKVVIPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSI 60

Query: 61  FEATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP 120
            EATKELFHLPIETR++NTSEKPYHGYFG YSFLPLYESMA+DNP  L++ Q+F NLMWP
Sbjct: 61  VEATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWP 120

Query: 121 A-GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPK 180
              N  FC TV  FSTLVAKLE+ VT+MVLESYGVSK V E  +EST +LLR FKYRV +
Sbjct: 121 TRDNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAE 180

Query: 181 QDETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDAL 240
           +DETD+GLHSHTDLTF SI+HQHLI GLQ++SL   +D++W+D+ P+   SFIVMAGDAL
Sbjct: 181 KDETDVGLHSHTDLTFFSIIHQHLISGLQIQSL---NDDQWLDVHPSSHCSFIVMAGDAL 240

Query: 241 MAWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFL 300
           MAWSN+RIR CRHRV+MRG+ETRYS+G+FSFK+GTI VP+  V+D +PL+YKPF+H++FL
Sbjct: 241 MAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFL 300

Query: 301 RYDKANASHKTCSRIKDYCGI 319
            YDKANASH T SRIKDYCG+
Sbjct: 301 TYDKANASHNTISRIKDYCGL 316

[Close]

BLAST of MS009445 vs. NCBI nr
Match: XP_022992484.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima])

HSP 1 Score: 451.8 bits (1161), Expect = 4.8e-123
Identity = 214/320 (66.88%), Postives = 266/320 (83.12%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSE-EKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60
           MG++ ++++P I+LS+ E L+ G+E W    K +RV LEEFGCF VV+E+VSLELHNS F
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPA 120
            A  ELF LPIETRI+NTSEK YHGYFGRYSFLPLYESMA++NPT L A Q+F NLMWPA
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 121 -GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQ 180
            GN  F ET+  FSTL+AKL++ +TRMVLESYGV+KQ+ +++I+ST +LLR FKYRVP++
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 181 DETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALM 240
            ETD+GLH+HTDLTF SI+HQHLIGGLQ++SL    D++WID++P+G  SF+VMAGDALM
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSL----DDQWIDLEPSGHCSFVVMAGDALM 240

Query: 241 AWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLR 300
           AWSNDRIR CRHRV M+ +ETRYS G+FSFK+GTISVPEELV+D +PL+YKPF+HF+FL 
Sbjct: 241 AWSNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLT 300

Query: 301 YDKANASHKTCSRIKDYCGI 319
           YDKA  S K   RI+DYC I
Sbjct: 301 YDKAKGSKKFLYRIRDYCSI 316

[Close]

BLAST of MS009445 vs. NCBI nr
Match: XP_023547382.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 450.3 bits (1157), Expect = 1.4e-122
Identity = 214/320 (66.88%), Postives = 265/320 (82.81%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSE-EKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60
           MG++ ++++P I+LS+ EKL+ GS+ W    K +RV LEEFGCF VV+E+VSLE+HNS F
Sbjct: 1   MGSETQARVPVIDLSKLEKLEAGSDAWGSTCKAMRVGLEEFGCFCVVFEEVSLEIHNSFF 60

Query: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP- 120
            A  ELF LPIETRI+NTSEK YHGYFGRYSFLPLYESMA+DNPT L A Q+F NLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 121 AGNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQ 180
            GN  F ET+  FSTL+AKL++ VTRMVLESYGVSKQ+ +++I+ST +LLR FKYRVP+Q
Sbjct: 121 QGNDRFSETIQRFSTLMAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 181 DETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALM 240
            ETD+GLH+HTDLTF SI+HQHLIGGLQ++SL    D++WID++P+   SF+VMAGDALM
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSL----DDQWIDLEPSSHCSFVVMAGDALM 240

Query: 241 AWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLR 300
           AWSNDRIR CRHRV M+ +ETRYS G+FSFK+GTISVPEELV+D +PL+YKPF+H++FL 
Sbjct: 241 AWSNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLT 300

Query: 301 YDKANASHKTCSRIKDYCGI 319
           +DKA  S K   RI+DYC I
Sbjct: 301 HDKAKGSKKFLYRIRDYCSI 316

[Close]

BLAST of MS009445 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-62
Identity = 127/305 (41.64%), Postives = 192/305 (62.95%), Query Frame = 0


Query: 8   KIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFEATKELFH 67
           ++P I+ S++ LK GS +W  +  ++  ALE++GCF   ++K+S+EL+ S+FEA ++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFE 72

Query: 68  LPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP-AGNKTFCE 127
           LPI T+ +N S KP+HGY        LYES+ +D+   L+ +  FT  +WP  GNK+  E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 128 TVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQD-----ET 187
           T+H FS  + +L+  V RM++ES+G+   + +  + ST +L R  KY  P  D     ET
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYI-DEHLNSTYYLTRLMKYTSPPDDDDDDEET 192

Query: 188 DLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAWS 247
            LGL SHTD   ++ILHQ+ + GL+VK+     D++WI + P+ + S +VM GD+L A  
Sbjct: 193 KLGLRSHTDKNIITILHQYQVDGLEVKT----KDDKWIKVKPS-QDSVLVMVGDSLCALL 252

Query: 248 NDRIRGCRHRVMMRGDETRYSLGLFSF-KSGT-ISVPEELVEDNHPLKYKPFDHFEFLRY 305
           N R+    HRV+M G +TRYS GLFS  K+G  I  PEELV+  HP  +KPF++ +FL +
Sbjct: 253 NGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHF 307

[Close]

BLAST of MS009445 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 8.4e-62
Identity = 126/306 (41.18%), Postives = 192/306 (62.75%), Query Frame = 0


Query: 8   KIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFEATKELFH 67
           ++P I+ S++ LK GS +W  +  ++  ALE++GCF   ++K+S+EL+ S+FEA ++LF 
Sbjct: 13  QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 72

Query: 68  LPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP-AGNKTFCE 127
           LPI T+ +N S KP+HGY        LYES+ +++   L+ +  FT  +WP  GNK+  E
Sbjct: 73  LPIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 132

Query: 128 TVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQD------E 187
           T+H FS  + +L+  V RM++ES+G+   + +  + ST +L R  KY  P  D      E
Sbjct: 133 TIHLFSEQLVELDLMVRRMIMESFGIENYI-DEHLNSTYYLTRLMKYTSPPDDDDDDDEE 192

Query: 188 TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAW 247
           T LGL SHTD   ++ILHQ+ + GL+VK+     D++WI + P+ + S +VM GD+L A 
Sbjct: 193 TKLGLRSHTDKNIITILHQYQVDGLEVKT----KDDKWIKVKPS-QDSVLVMVGDSLCAL 252

Query: 248 SNDRIRGCRHRVMMRGDETRYSLGLFSF-KSGT-ISVPEELVEDNHPLKYKPFDHFEFLR 305
            N R+    HRV+M G +TRYS GLFS  K+G  I  PEELV+  HP  +KPF++ +FL 
Sbjct: 253 LNGRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLH 308

[Close]

BLAST of MS009445 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 1.9e-61
Identity = 126/304 (41.45%), Postives = 192/304 (63.16%), Query Frame = 0


Query: 8   KIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFEATKELFH 67
           ++P I+ S++ LK GS +W  +  ++  ALE++GCF   ++K+S+EL+ S+FEA ++LF 
Sbjct: 6   QLPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFE 65

Query: 68  LPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP-AGNKTFCE 127
           LPI T+ +N S K +HGY        LYES+ +++   L+ +  FT  +WP  GNK+  E
Sbjct: 66  LPIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSISE 125

Query: 128 TVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQD----ETD 187
           T+H FS  + +L+  V RM++ES+G+ K + +  + ST +L R  KY  P  D    ET 
Sbjct: 126 TIHLFSEQLVELDLMVRRMIMESFGIEKYI-DEHLNSTYYLTRLMKYTSPPDDDDDEETK 185

Query: 188 LGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAWSN 247
           LGL SHTD   ++ILHQ+ + GL+VK+     D++WI + P+ + S +VM GD+L A  N
Sbjct: 186 LGLRSHTDKNIITILHQYQVDGLEVKT----KDDKWIKVKPS-QDSVLVMVGDSLCALLN 245

Query: 248 DRIRGCRHRVMMRGDETRYSLGLFSF-KSGT-ISVPEELVEDNHPLKYKPFDHFEFLRYD 305
            R+    HRV+M G +TRYS GLFS  K+G  I  PEELV+  HP  +KPF++ +FL + 
Sbjct: 246 GRLHSPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFF 299

[Close]

BLAST of MS009445 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.9e-54
Identity = 117/315 (37.14%), Postives = 174/315 (55.24%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60
           MG+ A  ++P I+LS + LK G+  W  +  ++R ALEE+GCF  V + VS EL  ++  
Sbjct: 1   MGSTAPLRLPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCN 60

Query: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAG 120
              EL +LP+ET++ N ++  Y G+    S   L E M V   T L+ ++ FTNLMWP G
Sbjct: 61  KGHELLNLPLETKMLNGNKPEYDGF---TSIPNLNEGMGVGRITDLEKVERFTNLMWPEG 120

Query: 121 NKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDE 180
           NK FCETV+ +   +A+++  +  MV ES+G+ K   +S  EST +LL   +Y+ P +D 
Sbjct: 121 NKDFCETVYSYGKRMAEVDHILKMMVFESFGMEKHF-DSFCESTNYLLHFMRYQQPGKDG 180

Query: 181 TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAW 240
               L  H D + L+I++Q+ + GL+ ++ D     EWI        + IV+ GD  MAW
Sbjct: 181 RSPALSLHKDKSILTIVNQNDVKGLEFETKDG----EWI---LPTADNHIVLLGDCFMAW 240

Query: 241 SNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYD 300
           SN R+    HRV +  ++ R S   FSF    I  P ELV++ HPL + PF+  E L Y 
Sbjct: 241 SNGRLHSPLHRVTLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYC 300

Query: 301 KANASHKTCSRIKDY 316
                 K    +K Y
Sbjct: 301 FTKEGAKAVCDLKQY 304

[Close]

BLAST of MS009445 vs. ExPASy Swiss-Prot
Match: Q945B5 (2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.8e-41
Identity = 117/432 (27.08%), Postives = 186/432 (43.06%), Query Frame = 0


Query: 8   KIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFEATKELFH 67
           ++P I L+++ L+ GS +W  +  ++R ALE+FGCF   Y+KVSLEL  SI +  +ELF 
Sbjct: 7   QLPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFA 66

Query: 68  LPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP--AGNKTFC 127
           LP+ET+ +N   KPY GY    +   L ES+ + N   L+ +  FT  +WP   GN+   
Sbjct: 67  LPVETKQRNVCPKPYVGYLNHNN---LSESLGISNANILENVNEFTQQLWPDGDGNENIS 126

Query: 128 ETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQ------- 187
           +T+  F+  + +++  V RMV+ES+G+ K + +  ++ST + +R  KY  P +       
Sbjct: 127 KTIQLFAEKLVEIDVMVRRMVMESFGIEKYI-DDHLKSTEYRMRLMKYIAPPEGDANTTV 186

Query: 188 ------------------------------------------------------------ 247
                                                                       
Sbjct: 187 DDYADLLAKLNIDGVEPNVGVKVNADISDDVNANPSVNAGVGANVNADTGVNDNLNVDAE 246

Query: 248 -------------------------------------------------DETDLGLHSHT 307
                                                            +E  LGL  HT
Sbjct: 247 ANGDANIAVGGGVNANTDLGVGVNVNSNVAVNAKTGATSGDDVEANDDNEEKKLGLPCHT 306

Query: 308 DLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAWSNDRIRGCR 319
           D    ++L QH I GL+VK+     DE+WI + P+  ++FIV+AGD+L A  N RIR   
Sbjct: 307 DKNLFTVLFQHEIEGLEVKT----KDEKWIRVKPS-PNTFIVIAGDSLCALMNGRIRAPY 366

[Close]

BLAST of MS009445 vs. ExPASy TrEMBL
Match: A0A6J1CXE5 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111015077 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 9.9e-183
Identity = 315/318 (99.06%), Postives = 316/318 (99.37%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60
           MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE
Sbjct: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60

Query: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAG 120
           ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAG
Sbjct: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAG 120

Query: 121 NKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDE 180
           NKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDE
Sbjct: 121 NKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDE 180

Query: 181 TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAW 240
           TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNN+D EWIDIDP GRSSFIVMAGDALMAW
Sbjct: 181 TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNNDGEWIDIDPVGRSSFIVMAGDALMAW 240

Query: 241 SNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYD 300
           SNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYD
Sbjct: 241 SNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYD 300

Query: 301 KANASHKTCSRIKDYCGI 319
           KANASHKTCSRIKDYCGI
Sbjct: 301 KANASHKTCSRIKDYCGI 318

[Close]

BLAST of MS009445 vs. ExPASy TrEMBL
Match: A0A1S3CDV2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.0e-127
Identity = 227/321 (70.72%), Postives = 270/321 (84.11%), Query Frame = 0


Query: 1   MGTQAKSK--IPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSI 60
           M  +AKSK  IPTI+LS  KL+VG++EW L  KE+R  LEEFGCF VVY+KVSLELHNSI
Sbjct: 1   MVCEAKSKVVIPTIDLS--KLEVGTKEWGLACKEMRFGLEEFGCFSVVYDKVSLELHNSI 60

Query: 61  FEATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP 120
            EATKELFHLPIETR++NTSEKPYHGYFG YSFLPLYESMA+DNP  L++ Q+F NLMWP
Sbjct: 61  VEATKELFHLPIETRMKNTSEKPYHGYFGGYSFLPLYESMAIDNPIQLKSTQSFANLMWP 120

Query: 121 A-GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPK 180
              N  FC TV  FSTLVAKLE+ VT+MVLESYGVSK V E  +EST +LLR FKYRV +
Sbjct: 121 TRDNNHFCVTVERFSTLVAKLEKMVTKMVLESYGVSKVVNEPIMESTNYLLRLFKYRVAE 180

Query: 181 QDETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDAL 240
           +DETD+GLHSHTDLTF SI+HQHLI GLQ++SL   +D++W+D+ P+   SFIVMAGDAL
Sbjct: 181 KDETDVGLHSHTDLTFFSIIHQHLISGLQIQSL---NDDQWLDVHPSSHCSFIVMAGDAL 240

Query: 241 MAWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFL 300
           MAWSN+RIR CRHRV+MRG+ETRYS+G+FSFK+GTI VP+  V+D +PL+YKPF+H++FL
Sbjct: 241 MAWSNERIRSCRHRVIMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFL 300

Query: 301 RYDKANASHKTCSRIKDYCGI 319
            YDKANASH T SRIKDYCG+
Sbjct: 301 TYDKANASHNTISRIKDYCGL 316

[Close]

BLAST of MS009445 vs. ExPASy TrEMBL
Match: A0A6J1JXN6 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 GN=LOC111488804 PE=3 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.3e-123
Identity = 214/320 (66.88%), Postives = 266/320 (83.12%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSE-EKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60
           MG++ ++++P I+LS+ E L+ G+E W    K +RV LEEFGCF VV+E+VSLELHNS F
Sbjct: 1   MGSETQARVPVIDLSKLETLEAGTEAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPA 120
            A  ELF LPIETRI+NTSEK YHGYFGRYSFLPLYESMA++NPT L A Q+F NLMWPA
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIENPTQLDAAQSFANLMWPA 120

Query: 121 -GNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQ 180
            GN  F ET+  FSTL+AKL++ +TRMVLESYGV+KQ+ +++I+ST +LLR FKYRVP++
Sbjct: 121 QGNNHFSETIQRFSTLMAKLDKIITRMVLESYGVTKQLIDTNIDSTANLLRFFKYRVPEE 180

Query: 181 DETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALM 240
            ETD+GLH+HTDLTF SI+HQHLIGGLQ++SL    D++WID++P+G  SF+VMAGDALM
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSL----DDQWIDLEPSGHCSFVVMAGDALM 240

Query: 241 AWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLR 300
           AWSNDRIR CRHRV M+ +ETRYS G+FSFK+GTISVPEELV+D +PL+YKPF+HF+FL 
Sbjct: 241 AWSNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHFDFLT 300

Query: 301 YDKANASHKTCSRIKDYCGI 319
           YDKA  S K   RI+DYC I
Sbjct: 301 YDKAKGSKKFLYRIRDYCSI 316

[Close]

BLAST of MS009445 vs. ExPASy TrEMBL
Match: A0A6J1GPR2 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662 GN=LOC111456352 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 6.8e-123
Identity = 216/320 (67.50%), Postives = 264/320 (82.50%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSE-EKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIF 60
           MG++ ++ +P I+LS+ EKL+ G++ W    K +RV LEEFGCF VV+E+VSLELHNS F
Sbjct: 1   MGSETQAGVPVIDLSKLEKLEAGTDAWGSTCKAMRVGLEEFGCFCVVFEEVSLELHNSFF 60

Query: 61  EATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWP- 120
            A  ELF LPIETRI+NTSEK YHGYFGRYSFLPLYESMA+DNPT L A Q+F NLMWP 
Sbjct: 61  GAVDELFQLPIETRIKNTSEKAYHGYFGRYSFLPLYESMAIDNPTQLDAAQSFANLMWPD 120

Query: 121 AGNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQ 180
            GN  F ET+  FSTLVAKL++ VTRMVLESYGVSKQ+ +++I+ST +LLR FKYRVP+Q
Sbjct: 121 QGNNRFSETIQRFSTLVAKLDKIVTRMVLESYGVSKQLIDTNIDSTANLLRFFKYRVPEQ 180

Query: 181 DETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALM 240
            ETD+GLH+HTDLTF SI+HQHLIGGLQ++SL    D++WID++P+   SFIVMAGDALM
Sbjct: 181 HETDVGLHAHTDLTFFSIIHQHLIGGLQIQSL----DDQWIDLEPSSHCSFIVMAGDALM 240

Query: 241 AWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLR 300
           AWSNDRIR CRHRV M+ +ETRYS G+FSFK+GTISVPEELV+D +PL+YKPF+H++FL 
Sbjct: 241 AWSNDRIRSCRHRVTMKAEETRYSAGMFSFKNGTISVPEELVDDANPLRYKPFNHYDFLT 300

Query: 301 YDKANASHKTCSRIKDYCGI 319
           +DKA  S K   RI+DYC I
Sbjct: 301 HDKAKGSKKFLYRIRDYCSI 316

[Close]

BLAST of MS009445 vs. ExPASy TrEMBL
Match: A0A6P5SM26 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Prunus avium OX=42229 GN=LOC110760070 PE=3 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 7.5e-106
Identity = 183/318 (57.55%), Postives = 243/318 (76.42%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60
           MG++ ++K+P ++LS E LK G+E WVL  K+++ ALEE+GCF  V+ KV L+LHNSIF 
Sbjct: 1   MGSETQAKLPVVDLSNENLKPGTEAWVLASKQVKYALEEYGCFEAVFHKVPLQLHNSIFS 60

Query: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAG 120
             ++LF LPIET+ Q TSE+PYH YFG+YSFLPLYES+ VDNPT+++  Q FT +MWPAG
Sbjct: 61  MVEDLFDLPIETKRQQTSERPYHSYFGQYSFLPLYESLGVDNPTTVEGAQGFTKIMWPAG 120

Query: 121 NKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDE 180
           N  FCE+   FS LVA+L++ VTRMV + YGV +++ +S + STT+LLRCFKYR  K++E
Sbjct: 121 NDHFCESAQSFSKLVAELDQMVTRMVFDIYGV-ERLYDSHMASTTYLLRCFKYREQKENE 180

Query: 181 TDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAW 240
           T++GL  H D TF SILHQ  + GLQ++S D     +WID +P+  SSF+VMAGDA MAW
Sbjct: 181 TNVGLRPHKDKTFTSILHQSQVHGLQIRSKDG----QWIDAEPS-PSSFLVMAGDAFMAW 240

Query: 241 SNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYD 300
           SNDR+R C H+V+M+ ++TRYSLGLFSF SG + VPEEL++D HPL YKPFDHF FLR+D
Sbjct: 241 SNDRVRSCDHQVIMKENKTRYSLGLFSFNSGILEVPEELIDDQHPLMYKPFDHFGFLRFD 300

Query: 301 KANASHKTCSRIKDYCGI 319
           +   + K+   IK YCG+
Sbjct: 301 QTPEAKKSECPIKAYCGV 312

[Close]

BLAST of MS009445 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 310.5 bits (794), Expect = 1.6e-84
Identity = 153/313 (48.88%), Postives = 206/313 (65.81%), Query Frame = 0


Query: 7   SKIPTIEL-SEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFEATKEL 66
           +K+P ++L S+++LK  +  W  + +E   ALEE+GCFL VY+ V+ +L +SIF A +EL
Sbjct: 8   TKVPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEEL 67

Query: 67  FHLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAGNKTFC 126
           F LP ET+ +N +EKPYHGY G+   +PL+E + VD  T+ +  Q FT+LMWP GN  FC
Sbjct: 68  FDLPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFC 127

Query: 127 ETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDETDLGL 186
            TVH FS  VA+L+  V RM+ E+YGV K   ES + S T+LL+  KY  P +  +    
Sbjct: 128 NTVHTFSNAVAELDRLVVRMIFENYGVEKHY-ESHVGSKTYLLKFLKYLAPPESISMPAF 187

Query: 187 HSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAWSNDRI 246
             HTD TFLSILHQ+ + GL+VKS D     EWI +    + S++VMAGD  M WSNDRI
Sbjct: 188 PQHTDKTFLSILHQNDVNGLEVKSKDG----EWISLQLPPK-SYVVMAGDISMGWSNDRI 247

Query: 247 RGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYDKANAS 306
           R C HRV M GD+TRY+LGLFSF +  +S+PEELV+D HPL YKPFD+   + +      
Sbjct: 248 RSCEHRVTMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEG 307

Query: 307 HKTCSRIKDYCGI 319
            +  S +K YCGI
Sbjct: 308 REANSTLKAYCGI 314

[Close]

BLAST of MS009445 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 4.5e-79
Identity = 142/311 (45.66%), Postives = 212/311 (68.17%), Query Frame = 0


Query: 8   KIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLV-VYEKVSLELHNSIFEATKELF 67
           KIPT++ S E LK G++ W    + IR ALEE+GCF++ + +K  L+L + +F +  +LF
Sbjct: 4   KIPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLF 63

Query: 68  HLPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAGNKTFCE 127
            LP +T+++N  +KP +GY G+   LPL+ES+ +DN TSL+A ++FT LMWP GN+ F E
Sbjct: 64  DLPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHFSE 123

Query: 128 TVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDETDLGLH 187
            ++ ++   A+L++ VTRMV +SY V K   +  IESTT+LLR  K R P  +   LG  
Sbjct: 124 CLYKYAEFAAELDQMVTRMVFQSYNVEKYY-DPYIESTTYLLRVLKNRAPNNENPTLGFV 183

Query: 188 SHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAWSNDRIR 247
           +HTD +F +ILHQ  + GL++++ +     E I+I+ +  S F+V+AGDALMAWSNDR+ 
Sbjct: 184 THTDKSFTTILHQDQVNGLEMETREG----ERININLSSPSLFMVVAGDALMAWSNDRVW 243

Query: 248 GCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLRYDKANASH 307
             RH+V++ G+  RYSLG+F+F +GT+ VPEEL++  HPL YKPFDH   LR+ + +  +
Sbjct: 244 SPRHQVLVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGY 303

Query: 308 KTCSRIKDYCG 318
           K+   IK YCG
Sbjct: 304 KSECPIKAYCG 309

[Close]

BLAST of MS009445 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 280.4 bits (716), Expect = 1.8e-75
Identity = 134/313 (42.81%), Postives = 207/313 (66.13%), Query Frame = 0


Query: 8   KIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFEATKELFH 67
           ++P I+ S + LK G  EW L   +++ AL+++G F   ++++  EL  S+F A +ELF 
Sbjct: 11  RLPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFD 70

Query: 68  LPIETRIQNTSEKPYHGYFGRYSFLPLYESMAVDNPTSLQAIQTFTNLMWPAGNKTFCET 127
           LP++T+++N S+KP+HGY G+Y  +PLYESM +D+    + +  FT  +WP GN +F  T
Sbjct: 71  LPLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISFSTT 130

Query: 128 VHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVPKQDETDLGLHS 187
           +  FS  +++L+ T+ RM++ES+G+ K + +  + ST +LLR  KY+ P  +ET +GL++
Sbjct: 131 IQSFSKKLSELDITIRRMIMESFGLDKYI-DEHLHSTNYLLRVMKYKGPDTEETKVGLNA 190

Query: 188 HTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALMAWSNDRIRG 247
           HTD   ++IL+Q+ + GL+V++ D N    WI + P  + SF VM GD+L A  N R+  
Sbjct: 191 HTDKNIVTILYQNHVEGLEVQTKDKN----WIKVKPT-QDSFTVMIGDSLYALLNGRLHS 250

Query: 248 CRHRVMMRGDETRYSLGLFSF-KSG-TISVPEELVEDNHPLKYKPFDHFEFLRYDKANAS 307
             HRVMM G ETRYSLGLFS  K+G  +S P+ELV++ HP  +KPFDH EFL++    A 
Sbjct: 251 PYHRVMMTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAG 310

Query: 308 HKTCSRIKDYCGI 319
            ++ S +K YCGI
Sbjct: 311 QRSQSALKTYCGI 317

[Close]

BLAST of MS009445 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 258.1 bits (658), Expect = 9.5e-69
Identity = 131/323 (40.56%), Postives = 200/323 (61.92%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60
           M +  +  +P ++ S +KL  G+  W+    ++R A+E  G F+  +  VS +L +++  
Sbjct: 1   MDSHEEQCLPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLA 60

Query: 61  ATKELFHLPIETRIQNTSEKPYHGYFGR-YSFLPLYESMAVDNPTSLQAIQTFTNLMWPA 120
             KE+++LP + +I+N + K  HGY         ++ES+ +D  T LQA + F+ L+WP 
Sbjct: 61  GMKEMYYLPDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQ 120

Query: 121 GNKTFCETVHDFSTLVAKLEETVTRMVLESYGV-SKQVCESSIESTTHLLRCFKYRVPKQ 180
           GN  FC+T H ++  +A+L++TV RM+ ESYG+  K+   S  EST +LLR   YR  + 
Sbjct: 121 GNDPFCQTTHMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQN 180

Query: 181 DETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDALM 240
            E + G  SHTD +F+SILHQ+ +GGLQ+K++      +W+  +P+  + F+V++G  L 
Sbjct: 181 GEANTGFVSHTDKSFMSILHQNHVGGLQLKTMTG----QWVGFNPS-PTRFVVLSGMGLT 240

Query: 241 AWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEFLR 300
           AWSNDRI+ C H+V+M  DE RYSLG FSF  GTI  PEELV+D HPL+Y PF+H   LR
Sbjct: 241 AWSNDRIKACYHKVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLR 300

Query: 301 -YDKANASHKTCSR--IKDYCGI 319
            Y+    S K  S   ++ YCG+
Sbjct: 301 FYESYINSLKKSSEDLLQIYCGV 318

[Close]

BLAST of MS009445 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.4e-66
Identity = 138/324 (42.59%), Postives = 184/324 (56.79%), Query Frame = 0


Query: 1   MGTQAKSKIPTIELSEEKLKVGSEEWVLIGKEIRVALEEFGCFLVVYEKVSLELHNSIFE 60
           MG     ++P I LS++ LK  +E W      +R A+E  G F+  Y      LH SI E
Sbjct: 1   MGGNETRELPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILE 60

Query: 61  ATKELFHLPIETRIQNTSEKPYHGYFGRYS-FLPLYESMAVDNPTSLQAIQTFTNLMWP- 120
           A KEL  LP+E +++N + K  HGY    S   P++E + +D    +Q  + F+ LMWP 
Sbjct: 61  AAKELLDLPVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPD 120

Query: 121 --AGNKTFCETVHDFSTLVAKLEETVTRMVLESYGVSKQVCESSIESTTHLLRCFKYRVP 180
               N  FCETVH ++ + A+LE+ V RM+ ESY V K   E  I  T +LLR  KYR  
Sbjct: 121 DHDDNDRFCETVHAYAKMQAELEQLVIRMLFESYNVEKYT-EKYIGGTRYLLRLLKYRRL 180

Query: 181 KQDETDLGLHSHTDLTFLSILHQHLIGGLQVKSLDNNDDEEWIDIDPAGRSSFIVMAGDA 240
              E +    SHTD +F+SILHQ+ I GL +KS     ++ W    P+  + F+V+AGDA
Sbjct: 181 PNGEPNRKFISHTDKSFISILHQNHITGLMLKS---EKEDVWYHFTPS-PTRFVVIAGDA 240

Query: 241 LMAWSNDRIRGCRHRVMMRGDETRYSLGLFSFKSGTISVPEELVEDNHPLKYKPFDHFEF 300
           +MAWSNDRI+ C H+V M   E RYSLG FSF+ G IS PEE+V+ +HPL Y PF H   
Sbjct: 241 IMAWSNDRIKACYHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGL 300

Query: 301 LRYDKANASHKTCSR--IKDYCGI 319
           L Y +    H    R   K YCGI
Sbjct: 301 LEYYETLEVHLKAHRTMTKAYCGI 319

The following BLAST results are available for this feature:

BLAST of MS009445 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145687.1	2.0e-182	99.06	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Momordica charantia]	[more]
XP_038898684.1	1.9e-127	70.72	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Benincasa hispida]	[more]
XP_008461057.1	4.2e-127	70.72	PREDICTED: probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 [Cucumi...	[more]
XP_022992484.1	4.8e-123	66.88	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita maxima]	[more]
XP_023547382.1	1.4e-122	66.88	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS009445 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	1.3e-62	41.64	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	8.4e-62	41.18	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
Q944Z5	1.9e-61	41.45	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
A0A0N9HQ36	4.9e-54	37.14	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q945B5	2.8e-41	27.08	2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP...	[more]

BLAST of MS009445 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CXE5	9.9e-183	99.06	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367...	[more]
A0A1S3CDV2	2.0e-127	70.72	probable 2-oxoglutarate-dependent dioxygenase AOP1 isoform X1 OS=Cucumis melo OX...	[more]
A0A6J1JXN6	2.3e-123	66.88	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1GPR2	6.8e-123	67.50	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Cucurbita moschata OX=3662...	[more]
A0A6P5SM26	7.5e-106	57.55	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Prunus avium OX=42229 GN=L...	[more]

BLAST of MS009445 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52800.1	1.6e-84	48.88	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52790.1	4.5e-79	45.66	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	1.8e-75	42.81	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	9.5e-69	40.56	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	3.4e-66	42.59	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 166..268 e-value: 1.7E-17 score: 63.7
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 2..299 e-value: 2.5E-57 score: 196.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 9..87 e-value: 5.7E-10 score: 40.0
None	No IPR available	PANTHER	PTHR47990:SF169	2-OXOGLUTARATE-DEPENDENT DIOXYGENASE	coord: 4..317
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..317
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 7..299
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 164..271 score: 10.63101

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009445.1	MS009445.1	mRNA