MS009444 (gene) Bitter gourd (TR) v1

Overview


Name	MS009444
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold813: 1321706 .. 1324386 (+)
RNA-Seq Expression	MS009444
Synteny	MS009444

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCCGTGTGGGACTTCCGGTAATAAATTTGAGCGGCGACGATCTGAGACCGGGCGGCGAGTGTTGGAAAACTACGGGGAAGAAAGTCCGAGAAGCGATGGAAGAGTACGGGTGTTTTGTGGCGGAATACAACGGGGTTTCGCCGGAGCTCCGGGAGCAGGTTTTCAAGGCATTGGAACAACTGTTTGATCTTCCAGTTGAGAGGAAGATGAAGAACAAGTACGAGAAGCCCTTCAATGGCTACGTTGGCCAGCTTCGTACTCTCCCTCTCCATGAAAGCTTGGGCATTGATGATGCCACAACCTTCCATGGAGTTCATAGCTTCACTAATCTCATGTGGCCCTCTGGAAATCACCATTTTAGGTCACTTCTTTTCCTCTTCAAACTATGTTTTTTACTCAAACTTCGTGATTAAATTGAAAGTTAAAAGTTAAAATTGATTCGGGTAAAACTCGAAATTTCTTTTCCTTCTAATGATTTTTTTGGGCTTGGTATCACAAATAGATGGGTAGGGTAAGTCAAACTTACCATTTTAATTTGTAAATAATTGTTCAACGAATTATGATTGGTTACATACCTTAGAAATTAATTTCGACACCAAATGTTGTAGGATGATGAGGACGTTGTCTAGTGAGATTAGTTGAGTATAACGGACACTCATGAATATAAAAAAAAAGAAGAAAAGAAATGGGATTCTCAATTAGCTAGCTAGAATGGTTTTTCTATTCCTTTGAATATATTTTTAGCTTGCCAAGGGACTATATTATTTTTTGTGGGTGTTGACTTTTATGTTTGTGCTAAGTACTATATATCTATTATTAATTTTTTTTTGTTGGGACGTTCTAACATTTTTTGATATAAAATATTTTTGGGGTCCGTTTGATAACGTTCATGTTTCTTGTTTCTTGTTTCTATTCCTTTATTTTTACGGAAGAAAAATGTATTGCATGGTTCTAATCTCTAAGAATTTAAGTATGATAAAATAATATTTTTATTTATATTTTGGAATAAATTGGAAATAAGAACAAGAAAGAGAAACTTTGGTTGTTTCCAAAATTTTTATACAATTGTGAGAATGTTATAAAAAAAAAAGGAGAAACAATAAACAAAGTTAATTATAAAATGCTTAGATTTCCTAAGAAAATTGAAAATGAGAAAAAGAAACTGAATGTTTACCAAACCAGCCTTAGTTGCTAACTATTAATAAAAATTTGACAGTTCTAAGTTTTTTGAAAACATAAAAAAATATTTATTTTCTATATTTCTCTCTCCTATTTGTTAAATATATAATTGTGGTAAGTAAAAGATACCAAAGTATTGTTATTTTAGTATCGAGATGTGTTTTGTAGTAGGTTTTTGGATGTTTTAATGATAATATTTTAGGTGATCTATGGATTGAATCTTTTTTGGTTAAAAATTCCCTTTAATTTGTTTCTTTTTTTTCTTTCAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGCATTAGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGAGTGGAGAAGTACTACGATTCTCATGCTTCTTCCATTACTTACCTTCTTCGAGTGTTGAAAAACAAAGCTCCTCAATCTGAAAACCCTAGTTTGTGCCTTGTGGATCACACTGATAAGAGCTTCACCACTATGCTTTATCAAGATCATATCAATGGTTTGGAGGTCAAAACTAAAGATGGGCAGTGGATTAAAGTCGAGATATCCTCACCTTCAAACTTCATCGTCATTGCGGGCGATGCGTTCAAGGTCGAGTATATATATATGACTAACTTTACGTTAAAGTGGTTGTTTCAGACTCCGGAGATAGTTAAGACTCAAGTATTAGAATGAAACTATAAAACTTAAGGGTCGGTTTTGGTAACGTTTCTTTTTTTTCTTTTTAAAGAAAGAGAAAAGCAAAACTATTTCTTCCATTTCTTAATCATTCCCTCAAATTTTAAATTAAAAAATTCAAGGACAAAATGCACTTTTATTCTTGAGATTTGAATTAAGTGTCAATTCAATTTTTGAGGTTTCAAAATGGACATCTTTAAAGTTTTAAAATGGTTCTAAATGACCGTCCTTCAAGTTAATTGACCCATAGTCAACTAAGAAAGATGATGTTGCCATTATTTGTTGGGTGGATTGTGATTATGTGGTAAAATATATTATTTTCAAATAAAGTAATCAAAATTATTATATATATATAATATGTCTTAATAAAAATGAGAAACGAGAAAAAAGAAATCGTTACTATAGATTTATGTTCTTAAAAAATAAGAACAAGAAACATAAACGTTACGAAAAGAGTACATAAACTTAACACATACTATCCAACCACCTTTTTGAAAAAGTTTCAATTCGTCGAGTTACTTATTTTCCGAGAACAAAACCCTAATTTATATAGTGTGTGTTTTGGGTTTTGGTTGAAAAGGCTTGGAGCAATGGCAGGATAGAATCACCAACTCATAAAGTGATGACAAGAGGGAATGAAGACAGGTACTCTGTTGGAATGTTTGGATTCAGCAATGGAACTGTGGAAGTGCCAAAAGAGCTGGTTGATCAAAACCATCCATTGAAATATAAGCCTTTTAACCACATTGGATTGCTTCGATTCTTCCAATCTCAAGAAGGTTTCAACTCCAAATCTCCCCTCAAAGATTACTGTGCTCTC

mRNA sequence

AGCCGTGTGGGACTTCCGGTAATAAATTTGAGCGGCGACGATCTGAGACCGGGCGGCGAGTGTTGGAAAACTACGGGGAAGAAAGTCCGAGAAGCGATGGAAGAGTACGGGTGTTTTGTGGCGGAATACAACGGGGTTTCGCCGGAGCTCCGGGAGCAGGTTTTCAAGGCATTGGAACAACTGTTTGATCTTCCAGTTGAGAGGAAGATGAAGAACAAGTACGAGAAGCCCTTCAATGGCTACGTTGGCCAGCTTCGTACTCTCCCTCTCCATGAAAGCTTGGGCATTGATGATGCCACAACCTTCCATGGAGTTCATAGCTTCACTAATCTCATGTGGCCCTCTGGAAATCACCATTTTAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGCATTAGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGAGTGGAGAAGTACTACGATTCTCATGCTTCTTCCATTACTTACCTTCTTCGAGTGTTGAAAAACAAAGCTCCTCAATCTGAAAACCCTAGTTTGTGCCTTGTGGATCACACTGATAAGAGCTTCACCACTATGCTTTATCAAGATCATATCAATGGTTTGGAGGTCAAAACTAAAGATGGGCAGTGGATTAAAGTCGAGATATCCTCACCTTCAAACTTCATCGTCATTGCGGGCGATGCGTTCAAGGCTTGGAGCAATGGCAGGATAGAATCACCAACTCATAAAGTGATGACAAGAGGGAATGAAGACAGGTACTCTGTTGGAATGTTTGGATTCAGCAATGGAACTGTGGAAGTGCCAAAAGAGCTGGTTGATCAAAACCATCCATTGAAATATAAGCCTTTTAACCACATTGGATTGCTTCGATTCTTCCAATCTCAAGAAGGTTTCAACTCCAAATCTCCCCTCAAAGATTACTGTGCTCTC

Coding sequence (CDS)

AGCCGTGTGGGACTTCCGGTAATAAATTTGAGCGGCGACGATCTGAGACCGGGCGGCGAGTGTTGGAAAACTACGGGGAAGAAAGTCCGAGAAGCGATGGAAGAGTACGGGTGTTTTGTGGCGGAATACAACGGGGTTTCGCCGGAGCTCCGGGAGCAGGTTTTCAAGGCATTGGAACAACTGTTTGATCTTCCAGTTGAGAGGAAGATGAAGAACAAGTACGAGAAGCCCTTCAATGGCTACGTTGGCCAGCTTCGTACTCTCCCTCTCCATGAAAGCTTGGGCATTGATGATGCCACAACCTTCCATGGAGTTCATAGCTTCACTAATCTCATGTGGCCCTCTGGAAATCACCATTTTAGTGCAACTTTGCATTCATTTGCAAAGATAGCAGAAGCATTAGACAAAATGGTGACCAAAATGATATTTGAAAGCTATGGAGTGGAGAAGTACTACGATTCTCATGCTTCTTCCATTACTTACCTTCTTCGAGTGTTGAAAAACAAAGCTCCTCAATCTGAAAACCCTAGTTTGTGCCTTGTGGATCACACTGATAAGAGCTTCACCACTATGCTTTATCAAGATCATATCAATGGTTTGGAGGTCAAAACTAAAGATGGGCAGTGGATTAAAGTCGAGATATCCTCACCTTCAAACTTCATCGTCATTGCGGGCGATGCGTTCAAGGCTTGGAGCAATGGCAGGATAGAATCACCAACTCATAAAGTGATGACAAGAGGGAATGAAGACAGGTACTCTGTTGGAATGTTTGGATTCAGCAATGGAACTGTGGAAGTGCCAAAAGAGCTGGTTGATCAAAACCATCCATTGAAATATAAGCCTTTTAACCACATTGGATTGCTTCGATTCTTCCAATCTCAAGAAGGTTTCAACTCCAAATCTCCCCTCAAAGATTACTGTGCTCTC

Protein sequence

SRVGLPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSPLKDYCAL

Homology

[Close]

BLAST of MS009444 vs. NCBI nr
Match: XP_022151695.1 (probable 2-oxoglutarate-dependent dioxygenase AOP1 [Momordica charantia])

HSP 1 Score: 641.7 bits (1654), Expect = 3.2e-180
Identity = 306/309 (99.03%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 1   SRVGLPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQ 60
           SRVGLPVINLSGDDLRPGGE WKTTGKKVREA+EEYGCF+AEYNGVSPELREQVFKALEQ
Sbjct: 27  SRVGLPVINLSGDDLRPGGERWKTTGKKVREAVEEYGCFMAEYNGVSPELREQVFKALEQ 86

Query: 61  LFDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHF 120
           LFDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHF
Sbjct: 87  LFDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHF 146

Query: 121 SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL 180
           SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL
Sbjct: 147 SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL 206

Query: 181 VDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPT 240
           VDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPT
Sbjct: 207 VDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPT 266

Query: 241 HKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSK 300
           HKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSK
Sbjct: 267 HKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSK 326

Query: 301 SPLKDYCAL 310
           SPLKDYCAL
Sbjct: 327 SPLKDYCAL 335

[Close]

BLAST of MS009444 vs. NCBI nr
Match: XP_038896850.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Benincasa hispida])

HSP 1 Score: 532.7 bits (1371), Expect = 2.1e-147
Identity = 249/307 (81.11%), Postives = 277/307 (90.23%), Query Frame = 0


Query: 4   GLPVINLSGDDLR-PGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLF 63
           GLPVIN SG+ ++  GG+ W+   ++VREA+EEYGCF+AEY+GVS ELR +V KA++ LF
Sbjct: 7   GLPVINFSGNGVKTEGGDEWRRRSREVREALEEYGCFIAEYDGVSRELRGEVMKAVKALF 66

Query: 64  DLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSA 123
           +LP+ERK KNKYEKPFNGYVG+LRTLPLHESLGIDDAT FHGV SFT+LMWPSGNHHFS 
Sbjct: 67  ELPIERKKKNKYEKPFNGYVGELRTLPLHESLGIDDATNFHGVQSFTHLMWPSGNHHFST 126

Query: 124 TLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVD 183
           TLHSFAKIAE LDKMVTKMIFESYGVEKY+ SHA SITYLLR+LKNKAP S NP+LCLVD
Sbjct: 127 TLHSFAKIAEELDKMVTKMIFESYGVEKYHKSHADSITYLLRILKNKAPHSSNPTLCLVD 186

Query: 184 HTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHK 243
           HTDKSFTTMLYQDHINGLEVKTK GQWI+V+ SSPS F+VIAGDAFKAWSN RIESPTHK
Sbjct: 187 HTDKSFTTMLYQDHINGLEVKTKHGQWIQVDNSSPSTFVVIAGDAFKAWSNDRIESPTHK 246

Query: 244 VMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSP 303
           VM RGNEDRYS+ MFGFSNG VEVPKELVDQ HPL+YKPF+HIGLL FF+S+EGFNSKSP
Sbjct: 247 VMIRGNEDRYSLAMFGFSNGIVEVPKELVDQTHPLRYKPFDHIGLLHFFRSKEGFNSKSP 306

Query: 304 LKDYCAL 310
           LKDYCAL
Sbjct: 307 LKDYCAL 313

[Close]

BLAST of MS009444 vs. NCBI nr
Match: XP_022991572.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita maxima])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 239/307 (77.85%), Postives = 274/307 (89.25%), Query Frame = 0


Query: 4   GLPVINLSGDDLR-PGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLF 63
           GLPVIN SGDD++  GGE W+    +VREA+EEYGCF+AEY G+SPELR QVFKA+E LF
Sbjct: 12  GLPVINFSGDDVKAEGGEVWRRRSGEVREALEEYGCFIAEYEGISPELRVQVFKAVEALF 71

Query: 64  DLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSA 123
           +LP+E K KN+YEKPFNGYVG+LRTLPLHESLGIDDAT   GV +FTNLMWP+GNHHFS+
Sbjct: 72  ELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTNLMWPNGNHHFSS 131

Query: 124 TLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVD 183
            LHSFAK AE LD+MVTKMIFESYGVEKY+++HA+SITYLLR LKNKAP S+NP+LCLVD
Sbjct: 132 NLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAPHSQNPTLCLVD 191

Query: 184 HTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHK 243
           HTDKSFTTM+YQDH NGLEV+TKDG+WI+V+ SSPS F+VIAGDAFKAWSN RIESPTHK
Sbjct: 192 HTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAWSNDRIESPTHK 251

Query: 244 VMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSP 303
           V  RGNEDRYS+ MFGFSNG VEVPKEL+DQ HPL+YKPF+HIGLL FF+S+EGF SKSP
Sbjct: 252 VTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFFRSKEGFKSKSP 311

Query: 304 LKDYCAL 310
           L+DYCAL
Sbjct: 312 LQDYCAL 318

[Close]

BLAST of MS009444 vs. NCBI nr
Match: XP_023548444.1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 520.0 bits (1338), Expect = 1.4e-143
Identity = 238/307 (77.52%), Postives = 274/307 (89.25%), Query Frame = 0


Query: 4   GLPVINLSGDDLR-PGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLF 63
           GLPVIN SGDD++  GGE W+    +VREA+EEYGCF+AEY G+SPELR +VFKA+E LF
Sbjct: 12  GLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEVFKAVEALF 71

Query: 64  DLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSA 123
           DLP+E K KN+YEKPFNGYVG+LRTLPLHESLGIDDAT  HGV +FT+LMWP+GNHHFS+
Sbjct: 72  DLPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIHGVQAFTHLMWPNGNHHFSS 131

Query: 124 TLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVD 183
            LHSFAK AE LD+MVTKMIFESYGVEKY+++HA+SITYLLR LKNK P S+NP+LCLVD
Sbjct: 132 NLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKTPHSQNPTLCLVD 191

Query: 184 HTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHK 243
           HTDKSFTTM+YQDH NGLEV+TKDG+WI+V+ SSPS F+VIAGDAFKAWSN RIESPTHK
Sbjct: 192 HTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAWSNDRIESPTHK 251

Query: 244 VMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSP 303
           V  RGNEDRYS+ MFGFSNG VEVPKEL+DQ HPL+YKPF+HIGLL FF+S+EGF SKSP
Sbjct: 252 VTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFFRSKEGFKSKSP 311

Query: 304 LKDYCAL 310
           L+DYCAL
Sbjct: 312 LQDYCAL 318

[Close]

BLAST of MS009444 vs. NCBI nr
Match: KAG6575571.1 (putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 518.5 bits (1334), Expect = 4.1e-143
Identity = 238/307 (77.52%), Postives = 274/307 (89.25%), Query Frame = 0


Query: 4   GLPVINLSGDDLR-PGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLF 63
           GLPVIN SGDD++  GGE W+    +VREA+EEYGCF+AEY G+SPELR +VFKA+E LF
Sbjct: 4   GLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEVFKAVEALF 63

Query: 64  DLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSA 123
           +LP+E K KN+YEKPFNGYVG+LRTLPLHESLGIDDAT   GV +FTNLMWP+GNHHFS+
Sbjct: 64  ELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTNLMWPNGNHHFSS 123

Query: 124 TLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVD 183
            LHSFAK AE LD+MVTKMIFESYGVEKY+++HA+SITYLLR LKNKAP S+NP+LCLVD
Sbjct: 124 NLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAPHSQNPTLCLVD 183

Query: 184 HTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHK 243
           HTDKSFTTM+YQDH NGLEV+TKDG+WI+V+ SSPS F+VIAGDAFKAWSN RIESPTHK
Sbjct: 184 HTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAWSNDRIESPTHK 243

Query: 244 VMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSP 303
           V  RGNEDRYS+ MFGFSNG VEVPKEL+DQ HPL+YKPF+HIGLL FF+S+EGF SKSP
Sbjct: 244 VTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFFRSKEGFKSKSP 303

Query: 304 LKDYCAL 310
           L+DYCAL
Sbjct: 304 LQDYCAL 310

[Close]

BLAST of MS009444 vs. ExPASy Swiss-Prot
Match: Q945B6 (Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=3702 GN=AOP1.2 PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.1e-66
Identity = 133/312 (42.63%), Postives = 189/312 (60.58%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LPVI+ S  +L+PG   W      V +A+E+YGCF A ++ +S EL   VF+A+E LF+L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVKADVLKALEDYGCFEAFFDKLSVELNRSVFEAMEDLFEL 73

Query: 65  PVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPS-GNHHFSAT 124
           P+  K +N   KPF+GY+       L+ESLGIDDA     V+ FT  +WP  GN   S T
Sbjct: 74  PIPTKQRNVSSKPFHGYLCH----NLYESLGIDDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 125 LHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAP-----QSENPSL 184
           +H F++    LD MV +MI ES+G+E Y D H +S  YL R++K  +P       E   L
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDEETKL 193

Query: 185 CLVDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIES 244
            L  HTDK+  T+L+Q  ++GLEVKTKD +WIKV+ S  S  +V+ GD+  A  NGR+ S
Sbjct: 194 GLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRLHS 253

Query: 245 PTHKVMTRGNEDRYSVGMFGF--SNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEG 304
           P H+V+  G + RYS G+F    +   ++ P+ELVD+ HP  +KPF +   L FFQ++ G
Sbjct: 254 PYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEAG 313

Query: 305 FNSKSPLKDYCA 309
             ++S L  + A
Sbjct: 314 RIAQSALHAFAA 320

[Close]

BLAST of MS009444 vs. ExPASy Swiss-Prot
Match: Q9ZTA3 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-66
Identity = 132/313 (42.17%), Postives = 189/313 (60.38%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LPVI+ S  +L+PG   W      V +A+E+YGCF A ++ +S EL   VF+A+E LF+L
Sbjct: 14  LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 73

Query: 65  PVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPS-GNHHFSAT 124
           P+  K +N   KPF+GY+       L+ESLGI+DA     V+ FT  +WP  GN   S T
Sbjct: 74  PIPTKQRNVSSKPFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 133

Query: 125 LHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAP------QSENPS 184
           +H F++    LD MV +MI ES+G+E Y D H +S  YL R++K  +P        E   
Sbjct: 134 IHLFSEQLVELDLMVRRMIMESFGIENYIDEHLNSTYYLTRLMKYTSPPDDDDDDDEETK 193

Query: 185 LCLVDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIE 244
           L L  HTDK+  T+L+Q  ++GLEVKTKD +WIKV+ S  S  +V+ GD+  A  NGR+ 
Sbjct: 194 LGLRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRLH 253

Query: 245 SPTHKVMTRGNEDRYSVGMFGF--SNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQE 304
           SP H+V+  G + RYS G+F    +   ++ P+ELVD+ HP  +KPF +   L FFQ++ 
Sbjct: 254 SPYHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEA 313

Query: 305 GFNSKSPLKDYCA 309
           G  ++S L  + A
Sbjct: 314 GRIAQSALHAFAA 321

[Close]

BLAST of MS009444 vs. ExPASy Swiss-Prot
Match: A0A0N9HQ36 (Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.5e-65
Identity = 126/305 (41.31%), Postives = 181/305 (59.34%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LPVI+LS  +L+PG   W +   +VREA+EEYGCF A  + VSPEL++ V     +L +L
Sbjct: 9   LPVIDLSMKNLKPGTTSWNSVRTQVREALEEYGCFEAVIDAVSPELQKAVCNKGHELLNL 68

Query: 65  PVERKMKNKYEKPFNGYVGQLRTLP-LHESLGIDDATTFHGVHSFTNLMWPSGNHHFSAT 124
           P+E KM N  +  ++G+     ++P L+E +G+   T    V  FTNLMWP GN  F  T
Sbjct: 69  PLETKMLNGNKPEYDGFT----SIPNLNEGMGVGRITDLEKVERFTNLMWPEGNKDFCET 128

Query: 125 LHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVDH 184
           ++S+ K    +D ++  M+FES+G+EK++DS   S  YLL  ++ + P  +  S  L  H
Sbjct: 129 VYSYGKRMAEVDHILKMMVFESFGMEKHFDSFCESTNYLLHFMRYQQPGKDGRSPALSLH 188

Query: 185 TDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHKV 244
            DKS  T++ Q+ + GLE +TKDG+WI   + +  N IV+ GD F AWSNGR+ SP H+V
Sbjct: 189 KDKSILTIVNQNDVKGLEFETKDGEWI---LPTADNHIVLLGDCFMAWSNGRLHSPLHRV 248

Query: 245 MTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSPL 304
               N+ R S   F F    +E P ELVD+ HPL + PF    LL +  ++EG  +   L
Sbjct: 249 TLVANQARLSTSSFSFPKDIIETPAELVDEEHPLLFNPFEITELLAYCFTKEGAKAVCDL 306

Query: 305 KDYCA 309
           K Y A
Sbjct: 309 KQYKA 306

[Close]

BLAST of MS009444 vs. ExPASy Swiss-Prot
Match: Q944Z5 (Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis thaliana OX=3702 GN=AOP1 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.0e-65
Identity = 129/299 (43.14%), Postives = 183/299 (61.20%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LPVI+ S  +L+PG   W      V +A+E+YGCF A ++ +S EL   VF+A+E LF+L
Sbjct: 7   LPVIDFSDQNLKPGSSKWDEVTADVLKALEDYGCFEASFDKLSVELNRSVFEAMEDLFEL 66

Query: 65  PVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPS-GNHHFSAT 124
           P+  K +N   K F+GY+       L+ESLGI+DA     V+ FT  +WP  GN   S T
Sbjct: 67  PIPTKQRNVSSKLFHGYLCH----NLYESLGINDANVLEKVNDFTQQLWPDHGNKSISET 126

Query: 125 LHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAP----QSENPSLC 184
           +H F++    LD MV +MI ES+G+EKY D H +S  YL R++K  +P      E   L 
Sbjct: 127 IHLFSEQLVELDLMVRRMIMESFGIEKYIDEHLNSTYYLTRLMKYTSPPDDDDDEETKLG 186

Query: 185 LVDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESP 244
           L  HTDK+  T+L+Q  ++GLEVKTKD +WIKV+ S  S  +V+ GD+  A  NGR+ SP
Sbjct: 187 LRSHTDKNIITILHQYQVDGLEVKTKDDKWIKVKPSQDS-VLVMVGDSLCALLNGRLHSP 246

Query: 245 THKVMTRGNEDRYSVGMFGF--SNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEG 297
            H+V+  G + RYS G+F    +   ++ P+ELVD+ HP  +KPF +   L FFQ++ G
Sbjct: 247 YHRVIMTGKKTRYSTGLFSIPKTGVIIDSPEELVDKEHPRIFKPFEYTDFLHFFQTEAG 300

[Close]

BLAST of MS009444 vs. ExPASy Swiss-Prot
Match: Q9ZTA2 (Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thaliana OX=3702 GN=AOP2 PE=2 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.3e-55
Identity = 136/393 (34.61%), Postives = 186/393 (47.33%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LP+INL+   L PG   W      VR+A+E++GCF A Y+ VS EL+E + K +E+LF L
Sbjct: 8   LPLINLADKTLEPGSSKWAEVRSDVRKALEDFGCFEASYDKVSLELQESIMKTMEELFAL 67

Query: 65  PVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWP--SGNHHFSA 124
           PVE K +N   KP   YVG L    L ESLGI +A     ++ FT  +WP   GN + S 
Sbjct: 68  PVETKQRNVCPKP---YVGYLNHNNLSESLGISNANILENINEFTQQLWPHGDGNENISK 127

Query: 125 TLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYL-------------------- 184
           T+  FA+    +D MV +M+ ES+G+EKY D H  S  Y                     
Sbjct: 128 TIQLFAEKLVEIDVMVRRMVMESFGIEKYIDDHLKSTAYATDELNIVGVEPNVGVKVNAD 187

Query: 185 ----------------------------LRVLKNKA------------------------ 244
                                       L V  N A                        
Sbjct: 188 ISDDVNANASVNAGVGANVNADTGVNDNLNVDANVAVGGGVNANTDLGVGVNVNSNVAVN 247

Query: 245 -----------PQSENPSLCLVDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSN 304
                        +E   L L  HTDK+  T+L+Q  I GLEVKTKD +WI+V+  SP+ 
Sbjct: 248 AKTGGDDVEANDDNEEKKLGLPCHTDKNLFTVLFQHEIEGLEVKTKDEKWIRVK-PSPNT 307

Query: 305 FIVIAGDAFKAWSNGRIESPTHKV-MTRGNEDRYSVGMFGF--SNGTVEVPKELVDQNHP 310
           FIVIAGD+  A  NGRI +P H+V +T     RY+  +F     +  +E PKELVD+ HP
Sbjct: 308 FIVIAGDSLCALMNGRIRAPYHRVRVTEKKRTRYTAAIFTCPKPDYVIEAPKELVDEKHP 367

[Close]

BLAST of MS009444 vs. ExPASy TrEMBL
Match: A0A6J1DBW9 (probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=3673 GN=LOC111019607 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.5e-180
Identity = 306/309 (99.03%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 1   SRVGLPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQ 60
           SRVGLPVINLSGDDLRPGGE WKTTGKKVREA+EEYGCF+AEYNGVSPELREQVFKALEQ
Sbjct: 27  SRVGLPVINLSGDDLRPGGERWKTTGKKVREAVEEYGCFMAEYNGVSPELREQVFKALEQ 86

Query: 61  LFDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHF 120
           LFDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHF
Sbjct: 87  LFDLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHF 146

Query: 121 SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL 180
           SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL
Sbjct: 147 SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL 206

Query: 181 VDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPT 240
           VDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPT
Sbjct: 207 VDHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPT 266

Query: 241 HKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSK 300
           HKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSK
Sbjct: 267 HKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSK 326

Query: 301 SPLKDYCAL 310
           SPLKDYCAL
Sbjct: 327 SPLKDYCAL 335

[Close]

BLAST of MS009444 vs. ExPASy TrEMBL
Match: A0A6J1JM78 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima OX=3661 GN=LOC111488145 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.2e-144
Identity = 239/307 (77.85%), Postives = 274/307 (89.25%), Query Frame = 0


Query: 4   GLPVINLSGDDLR-PGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLF 63
           GLPVIN SGDD++  GGE W+    +VREA+EEYGCF+AEY G+SPELR QVFKA+E LF
Sbjct: 12  GLPVINFSGDDVKAEGGEVWRRRSGEVREALEEYGCFIAEYEGISPELRVQVFKAVEALF 71

Query: 64  DLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSA 123
           +LP+E K KN+YEKPFNGYVG+LRTLPLHESLGIDDAT   GV +FTNLMWP+GNHHFS+
Sbjct: 72  ELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTNLMWPNGNHHFSS 131

Query: 124 TLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVD 183
            LHSFAK AE LD+MVTKMIFESYGVEKY+++HA+SITYLLR LKNKAP S+NP+LCLVD
Sbjct: 132 NLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAPHSQNPTLCLVD 191

Query: 184 HTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHK 243
           HTDKSFTTM+YQDH NGLEV+TKDG+WI+V+ SSPS F+VIAGDAFKAWSN RIESPTHK
Sbjct: 192 HTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAWSNDRIESPTHK 251

Query: 244 VMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSP 303
           V  RGNEDRYS+ MFGFSNG VEVPKEL+DQ HPL+YKPF+HIGLL FF+S+EGF SKSP
Sbjct: 252 VTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFFRSKEGFKSKSP 311

Query: 304 LKDYCAL 310
           L+DYCAL
Sbjct: 312 LQDYCAL 318

[Close]

BLAST of MS009444 vs. ExPASy TrEMBL
Match: A0A6J1GRH7 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschata OX=3662 GN=LOC111456372 PE=4 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 7.5e-143
Identity = 237/307 (77.20%), Postives = 274/307 (89.25%), Query Frame = 0


Query: 4   GLPVINLSGDDLR-PGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLF 63
           GLPVIN SGDD++  GGE W+    +VREA+EEYGCF+AEY G+SPELR +VFKA+E LF
Sbjct: 12  GLPVINFSGDDVKAEGGEGWRRRSGEVREALEEYGCFIAEYEGISPELRVEVFKAVEALF 71

Query: 64  DLPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSA 123
           +LP+E K KN+YEKPFNGYVG+LRTLPLHESLGIDDAT   GV +FT+LMWP+GNHHFS+
Sbjct: 72  ELPIETKKKNRYEKPFNGYVGELRTLPLHESLGIDDATNIDGVQAFTHLMWPNGNHHFSS 131

Query: 124 TLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVD 183
            LHSFAK AE LD+MVTKMIFESYGVEKY+++HA+SITYLLR LKNKAP S+NP+LCLVD
Sbjct: 132 NLHSFAKKAEELDQMVTKMIFESYGVEKYHETHAASITYLLRALKNKAPHSQNPTLCLVD 191

Query: 184 HTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHK 243
           HTDKSFTTM+YQDH NGLEV+TKDG+WI+V+ SSPS F+VIAGDAFKAWSN RIESPTHK
Sbjct: 192 HTDKSFTTMVYQDHTNGLEVQTKDGRWIQVDNSSPSTFVVIAGDAFKAWSNDRIESPTHK 251

Query: 244 VMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSP 303
           V  RGNEDRYS+ MFGFSNG VEVPKEL+DQ HPL+YKPF+HIGLL FF+S+EGF SKSP
Sbjct: 252 VTIRGNEDRYSLAMFGFSNGIVEVPKELIDQTHPLRYKPFDHIGLLHFFRSKEGFKSKSP 311

Query: 304 LKDYCAL 310
           L+DYCAL
Sbjct: 312 LQDYCAL 318

[Close]

BLAST of MS009444 vs. ExPASy TrEMBL
Match: A0A1S3CDV1 (probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499759 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 1.0e-128
Identity = 217/306 (70.92%), Postives = 257/306 (83.99%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGEC-WKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFD 64
           LPVIN S + ++  G   W+ T KKVREA+EEYGCF+A+Y GV  ELRE+V KA+++LF+
Sbjct: 10  LPVINFSDEKVKAEGSVEWRRTSKKVREALEEYGCFIAKYEGVGKELREEVLKAVKELFE 69

Query: 65  LPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSAT 124
           LP+ERK  NKYEKPFNGYVG+LRTLPLHESLGIDDAT F+GV SF++LMWP GNHHFSAT
Sbjct: 70  LPIERKKMNKYEKPFNGYVGELRTLPLHESLGIDDATNFNGVQSFSHLMWPEGNHHFSAT 129

Query: 125 LHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVDH 184
           LHSFAK AE LDKMVTKMIFESYGVEKYY+SHA SITYLLR+LKNKAPQS +P+LCLVDH
Sbjct: 130 LHSFAKRAEELDKMVTKMIFESYGVEKYYNSHADSITYLLRILKNKAPQSPDPTLCLVDH 189

Query: 185 TDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHKV 244
           TDKSFTTM+YQDHINGLE+KTK+GQWI+V+ SSPS+F+VIAGDAFKAWSN RI S  H+V
Sbjct: 190 TDKSFTTMIYQDHINGLELKTKNGQWIQVDNSSPSSFVVIAGDAFKAWSNERIRSCRHRV 249

Query: 245 MTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSPL 304
           + RG E RYS+GMF F NGT+EVP+  VD  +PL+YKPFNH   L + ++    N+ S +
Sbjct: 250 IMRGEETRYSIGMFSFKNGTIEVPQGFVDDANPLRYKPFNHYDFLTYDKANASHNTISRI 309

Query: 305 KDYCAL 310
           KDYC L
Sbjct: 310 KDYCGL 315

[Close]

BLAST of MS009444 vs. ExPASy TrEMBL
Match: A0A6A1W048 (Putative 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Morella rubra OX=262757 GN=CJ030_MR3G026499 PE=3 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 7.3e-114
Identity = 191/305 (62.62%), Postives = 243/305 (79.67%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LPV++ S ++L+PG   W +T K+V+ A+E+YGCFV  Y+ V  ELR++VF ALEQLFDL
Sbjct: 9   LPVLDFSSENLKPGTSSWLSTRKEVQRALEDYGCFVLVYDRVPSELRKEVFGALEQLFDL 68

Query: 65  PVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSATL 124
           P E KMKNKYEKP NGYVGQ+  LPLHES+GID+ATT  G  SFTNLMWP+GN+ F + +
Sbjct: 69  PTETKMKNKYEKPLNGYVGQIPKLPLHESMGIDNATTVEGTQSFTNLMWPNGNNGFCSCM 128

Query: 125 HSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVDHT 184
           HS++K    LD+MV +MI ESYGVE++YDS+  SITYLLRVLKN+ PQ   P+L  V HT
Sbjct: 129 HSYSKQLAELDQMVARMIHESYGVEQHYDSYIGSITYLLRVLKNRVPQGNEPNLAFVTHT 188

Query: 185 DKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHKVM 244
           DKSFTT+L+Q+ +NGLEV+TK+G+WI VE+ +PS+F+VIAGDA  AWSN R+ SPTH+V+
Sbjct: 189 DKSFTTILHQNQVNGLEVETKNGEWISVEL-TPSSFVVIAGDALMAWSNDRVLSPTHRVI 248

Query: 245 TRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSPLK 304
            RGNE RYS+  F FS+G VEVPKELVD  HPL+YKPF+HIGLL FF++ EG+ SK P+K
Sbjct: 249 MRGNEARYSLAQFSFSSGIVEVPKELVDDEHPLRYKPFDHIGLLHFFRTDEGYKSKCPIK 308

Query: 305 DYCAL 310
            YC +
Sbjct: 309 AYCGV 312

[Close]

BLAST of MS009444 vs. TAIR 10
Match: AT1G52790.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 395.6 bits (1015), Expect = 3.7e-110
Identity = 172/304 (56.58%), Postives = 236/304 (77.63%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSP-ELREQVFKALEQLFD 64
           +P ++ S +DL+PG + W++T + +R+A+EEYGCF+ +    +P +L ++VF +L  LFD
Sbjct: 5   IPTLDFSREDLKPGTKYWESTRENIRQALEEYGCFIIDLKDKTPLDLLDRVFGSLVDLFD 64

Query: 65  LPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSAT 124
           LP + KMKNKY+KP NGYVGQ+  LPLHESLGID+AT+     SFT LMWP GN HFS  
Sbjct: 65  LPTQTKMKNKYDKPLNGYVGQIPALPLHESLGIDNATSLEATRSFTGLMWPQGNEHFSEC 124

Query: 125 LHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVDH 184
           L+ +A+ A  LD+MVT+M+F+SY VEKYYD +  S TYLLRVLKN+AP +ENP+L  V H
Sbjct: 125 LYKYAEFAAELDQMVTRMVFQSYNVEKYYDPYIESTTYLLRVLKNRAPNNENPTLGFVTH 184

Query: 185 TDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHKV 244
           TDKSFTT+L+QD +NGLE++T++G+ I + +SSPS F+V+AGDA  AWSN R+ SP H+V
Sbjct: 185 TDKSFTTILHQDQVNGLEMETREGERININLSSPSLFMVVAGDALMAWSNDRVWSPRHQV 244

Query: 245 MTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSPL 304
           +  G  DRYS+GMF F+NGT++VP+EL+D  HPL YKPF+H+GLLRF+++  G+ S+ P+
Sbjct: 245 LVSGETDRYSLGMFAFNNGTLQVPEELIDHQHPLMYKPFDHLGLLRFYRTDTGYKSECPI 304

Query: 305 KDYC 308
           K YC
Sbjct: 305 KAYC 308

[Close]

BLAST of MS009444 vs. TAIR 10
Match: AT1G52800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 1.5e-87
Identity = 143/306 (46.73%), Postives = 208/306 (67.97%), Query Frame = 0


Query: 5   LPVINL-SGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFD 64
           +P+++L S  +L+P    W++  ++  EA+EEYGCF+A Y+GV+ +L + +F A E+LFD
Sbjct: 10  VPILDLTSQQELKPNTSTWRSVSREACEALEEYGCFLAVYDGVTQQLDDSIFAAAEELFD 69

Query: 65  LPVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSAT 124
           LP E K KN  EKP++GYVGQ+  +PLHE LG+D  T       FT+LMWP GN  F  T
Sbjct: 70  LPTETKKKNVNEKPYHGYVGQMPVIPLHEGLGVDYVTNKEIAQRFTHLMWPQGNDRFCNT 129

Query: 125 LHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVDH 184
           +H+F+     LD++V +MIFE+YGVEK+Y+SH  S TYLL+ LK  AP           H
Sbjct: 130 VHTFSNAVAELDRLVVRMIFENYGVEKHYESHVGSKTYLLKFLKYLAPPESISMPAFPQH 189

Query: 185 TDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHKV 244
           TDK+F ++L+Q+ +NGLEVK+KDG+WI +++  P +++V+AGD    WSN RI S  H+V
Sbjct: 190 TDKTFLSILHQNDVNGLEVKSKDGEWISLQL-PPKSYVVMAGDISMGWSNDRIRSCEHRV 249

Query: 245 MTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSPL 304
              G++ RY++G+F F    V +P+ELVD  HPL YKPF++I L+ F+ ++EG  + S L
Sbjct: 250 TMEGDKTRYTLGLFSFLTDLVSIPEELVDDKHPLMYKPFDNIALINFYTTKEGREANSTL 309

Query: 305 KDYCAL 310
           K YC +
Sbjct: 310 KAYCGI 314

[Close]

BLAST of MS009444 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.6e-81
Identity = 144/307 (46.91%), Postives = 203/307 (66.12%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LPVI+ S  +L+PG   W  T   V++A+++YG F A ++ +  ELR+ VF ALE+LFDL
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 65  PVERKMKNKYEKPFNGYVGQLRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSATL 124
           P++ K++N  +KPF+GYVGQ   +PL+ES+GIDD+     V +FT  +WP GN  FS T+
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKVDAFTEKLWPQGNISFSTTI 131

Query: 125 HSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCLVDHT 184
            SF+K    LD  + +MI ES+G++KY D H  S  YLLRV+K K P +E   + L  HT
Sbjct: 132 QSFSKKLSELDITIRRMIMESFGLDKYIDEHLHSTNYLLRVMKYKGPDTEETKVGLNAHT 191

Query: 185 DKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTHKVM 244
           DK+  T+LYQ+H+ GLEV+TKD  WIKV+ +  S F V+ GD+  A  NGR+ SP H+VM
Sbjct: 192 DKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDS-FTVMIGDSLYALLNGRLHSPYHRVM 251

Query: 245 TRGNEDRYSVGMFGF--SNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKSP 304
             G E RYS+G+F    +   V  P ELVD+ HP  +KPF+H+  L+F+ ++ G  S+S 
Sbjct: 252 MTGTETRYSLGLFSIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQRSQSA 311

Query: 305 LKDYCAL 310
           LK YC +
Sbjct: 312 LKTYCGI 317

[Close]

BLAST of MS009444 vs. TAIR 10
Match: AT1G80320.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 132/311 (42.44%), Postives = 191/311 (61.41%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LP+++ S D L  G   W TT   VR AME  G FVAE++GVS +LR+ +   +++++ L
Sbjct: 9   LPILDFSSDKLVRGTSHWITTRDDVRRAMEGQGWFVAEFSGVSSDLRDNLLAGMKEMYYL 68

Query: 65  PVERKMKNKYEKPFNGYVGQ-LRTLPLHESLGIDDATTFHGVHSFTNLMWPSGNHHFSAT 124
           P + K+KN+  K  +GY+   +    +HESLGID AT       F+ L+WP GN  F  T
Sbjct: 69  PDQIKIKNENHKASHGYMSMVVDDYRIHESLGIDYATELQACKDFSKLLWPQGNDPFCQT 128

Query: 125 LHSFAKIAEALDKMVTKMIFESYGVE--KYYDSHASSITYLLRVLKNKAPQSENPSLCLV 184
            H +A     LD+ V +M++ESYG++  K+  SH+ S  YLLR+L  +  Q+   +   V
Sbjct: 129 THMYAMTMAELDQTVMRMLYESYGMDEKKHSVSHSESTRYLLRMLSYRRQQNGEANTGFV 188

Query: 185 DHTDKSFTTMLYQDHINGLEVKTKDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESPTH 244
            HTDKSF ++L+Q+H+ GL++KT  GQW+     SP+ F+V++G    AWSN RI++  H
Sbjct: 189 SHTDKSFMSILHQNHVGGLQLKTMTGQWVGFN-PSPTRFVVLSGMGLTAWSNDRIKACYH 248

Query: 245 KVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGFNSKS 304
           KV+   +E RYS+G F F  GT+  P+ELVD  HPL+Y PF H GLLRF++S      KS
Sbjct: 249 KVVMSADEIRYSLGFFSFHKGTIRTPEELVDDQHPLRYNPFEHDGLLRFYESYINSLKKS 308

Query: 305 P---LKDYCAL 310
               L+ YC +
Sbjct: 309 SEDLLQIYCGV 318

[Close]

BLAST of MS009444 vs. TAIR 10
Match: AT1G15540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 3.7e-70
Identity = 132/312 (42.31%), Postives = 189/312 (60.58%), Query Frame = 0


Query: 5   LPVINLSGDDLRPGGECWKTTGKKVREAMEEYGCFVAEYNGVSPELREQVFKALEQLFDL 64
           LP+INLS  +L+   E W  T   VREAME +G FVAEYN     L + + +A ++L DL
Sbjct: 9   LPIINLSDKNLKQNTELWSFTRDSVREAMEHHGWFVAEYNNFPTGLHQSILEAAKELLDL 68

Query: 65  PVERKMKNKYEKPFNGYVGQLRT-LPLHESLGIDDATTFHGVHSFTNLMWP---SGNHHF 124
           PVE K+KN+  K  +GY+  +    P+HE LGID          F+ LMWP     N  F
Sbjct: 69  PVEIKLKNENHKAGHGYITMMSDGQPVHEGLGIDQVNDVQQCRGFSRLMWPDDHDDNDRF 128

Query: 125 SATLHSFAKIAEALDKMVTKMIFESYGVEKYYDSHASSITYLLRVLKNKAPQSENPSLCL 184
             T+H++AK+   L+++V +M+FESY VEKY + +     YLLR+LK +   +  P+   
Sbjct: 129 CETVHAYAKMQAELEQLVIRMLFESYNVEKYTEKYIGGTRYLLRLLKYRRLPNGEPNRKF 188

Query: 185 VDHTDKSFTTMLYQDHINGLEVKT-KDGQWIKVEISSPSNFIVIAGDAFKAWSNGRIESP 244
           + HTDKSF ++L+Q+HI GL +K+ K+  W      SP+ F+VIAGDA  AWSN RI++ 
Sbjct: 189 ISHTDKSFISILHQNHITGLMLKSEKEDVWYHF-TPSPTRFVVIAGDAIMAWSNDRIKAC 248

Query: 245 THKVMTRGNEDRYSVGMFGFSNGTVEVPKELVDQNHPLKYKPFNHIGLLRFFQSQEGF-- 304
            HKV     E RYS+G F F  G +  P+E+VD++HPL Y PF+H GLL ++++ E    
Sbjct: 249 YHKVEMESVEMRYSLGFFSFQEGMISTPEEMVDKDHPLAYNPFHHDGLLEYYETLEVHLK 308

Query: 305 NSKSPLKDYCAL 310
             ++  K YC +
Sbjct: 309 AHRTMTKAYCGI 319

The following BLAST results are available for this feature:

BLAST of MS009444 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022151695.1	3.2e-180	99.03	probable 2-oxoglutarate-dependent dioxygenase AOP1 [Momordica charantia]	[more]
XP_038896850.1	2.1e-147	81.11	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Benincasa hispida]	[more]
XP_022991572.1	1.1e-143	77.85	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita maxima]	[more]
XP_023548444.1	1.4e-143	77.52	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 [Cucurbita pepo subs...	[more]
KAG6575571.1	4.1e-143	77.52	putative 2-oxoglutarate-dependent dioxygenase AOP1, partial [Cucurbita argyrospe...	[more]

BLAST of MS009444 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q945B6	1.1e-66	42.63	Probable 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Arabidopsis thaliana OX=...	[more]
Q9ZTA3	2.4e-66	42.17	Probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Arabidopsis thaliana OX=37...	[more]
A0A0N9HQ36	3.5e-65	41.31	Deoxypodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=2-ODD PE=...	[more]
Q944Z5	6.0e-65	43.14	Probable 2-oxoglutarate-dependent dioxygenase AOP1 (Fragment) OS=Arabidopsis tha...	[more]
Q9ZTA2	3.3e-55	34.61	Probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Arabidopsis thali...	[more]

BLAST of MS009444 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBW9	1.5e-180	99.03	probable 2-oxoglutarate-dependent dioxygenase AOP1 OS=Momordica charantia OX=367...	[more]
A0A6J1JM78	5.2e-144	77.85	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita maxima ...	[more]
A0A6J1GRH7	7.5e-143	77.20	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 OS=Cucurbita moschat...	[more]
A0A1S3CDV1	1.0e-128	70.92	probable inactive 2-oxoglutarate-dependent dioxygenase AOP2 isoform X2 OS=Cucumi...	[more]
A0A6A1W048	7.3e-114	62.62	Putative 2-oxoglutarate-dependent dioxygenase AOP1.2 OS=Morella rubra OX=262757 ...	[more]

BLAST of MS009444 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52790.1	3.7e-110	56.58	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52800.1	1.5e-87	46.73	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G52820.1	1.6e-81	46.91	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G80320.1	1.7e-70	42.44	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G15540.1	3.7e-70	42.31	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 5..83 e-value: 3.5E-14 score: 53.6
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 171..257 e-value: 1.5E-15 score: 57.5
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..292 e-value: 1.0E-57 score: 197.9
None	No IPR available	PANTHER	PTHR47990:SF153	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN	coord: 4..308
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 4..308
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 3..294
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 161..262 score: 8.032269

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009444.1	MS009444.1	mRNA