MS009421 (gene) Bitter gourd (TR) v1

Overview


Name	MS009421
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA replication complex GINS protein PSF1-like
Location	scaffold813: 1201987 .. 1204602 (+)
RNA-Seq Expression	MS009421
Synteny	MS009421

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATGGAAGAAAAGCATGCCAGTTGGTGAAAGAGCTTGCAAACGGCGAGAAAGGTCAGCTAACGCACTTCAATGTAAGTCCACATGCTCGTTTATGTATATGTGTACCTATTGAGATTGTATTATATTGGCTACCGAGTTTTGCCCTTGCAATTTCGTTTCCATTTGATGCTTGCAGCTATTTGCATTATTTGGAGATCTAGAAGGGCTAATTTTATAGTTGATTAAATGTTTTGCACTTTGTTCCATCGGATGATTAAGTCACTTGAAGAATCGTTTACTTCGATAAATAAACTCAAATTTTCAAATTCATTTTCAAAGACTGTATTTTGCTCGTTTCTTTTTTCCAGCACTTCCAGGTTTCTGTGAAGTGATTATTTTACACTAATATACATATTCTTATAAACTAGCTTGTTTTTTATTGGATGCTCATTTCTGATGGCTAATTATTAAGCACTTTGAGTAAGTAGATGTCTTGAAAAGAATTTATGGTGGCTTAAATCACCTTGTTCAATGTCTCTGTGTTCAGATTTTTAATCTGTTTTCTATAGTAAATTCAGATTGCATCTAATATTTTGCTCTTTTGTTTGTACCTGTTTTTTCCCTGTGTTTCTTCAGAGCGATCTATTTGATCAGGCTATTTCAGAATGCGAGCAGCATCATCTTGAGCTTCAGTCCTTGATGAGGTTATAATATTCTACCTTTTCATTTACCTATTATTTGATAATGATAAGTTAGTTTAAGTGGTAATTGATCTGTTTTCGGTTGATCATGATTGGTAGGAAAAAAAGGTAGTTAATTCTAAGAGCACGCTCATTTGGTTAATAATAGTTCAATATATATAAGGAGAGCAATCTTGAGGAATGACAGAGGTTCACATTTTCACGAGTAGTGATTTTATCACGGTTAATGCTGATGGTCTCGTATGGGCCACCTAGTGAGTACCATCGACTGAGATTTCTTTTATCCAATTTCCGTCCATGAGATTTCTTTTATCCAATTTCCGTCCATTCTGCCCTCCATTCCATAATATGGTATGAAAGCTTTCAAGAACAGAAGTCAACTCAAGGCTTCATTTTATTGGCAGTAGCACTTGGGACCTATCTTCATCCCAGGAACAGTGTCCTTGGTCGATGATAGAACTATAAGATATGAATTTCAGGTACCTTTTACTCGTTAACAAGTTTGATAGTTTGAGAAAATCTTCATCCATTTCTCTTGAAAATCCACACCGTTATATCAGTTGCAATATGTATCCCAGGAACTATCCCAGTAAGTTCACTAGAGTCCTGAACTTGCTACCGTATATTCTCGACTCATTGCATTTAAGATTGTGTCAAAAATCTCCCTGATTGAACTGTCACATGTAGAAAGGAATGAAGTCATGGATGGCACAAGACATTTGCTCTCTCTTTAGTAACAGTTGCATCTAATAAAATTGGAAGGTTAACCCAAATGCTACCTCTTGTTGTGTCACTTAAACAAATAACCAAATGCTGAAAGCTGACCAGCCATGTATTCACTGTCGCTCTGGTTGTGAAAGCGCCTAAACCAATTAATTATAACTCTTCAATTTATGTAAGTGAGCAAAGAGAAATAGTGCCACTTGTATGCGTTTAAGTACTGGTCTAGAATCACTGGCTACCTAAATTAAAAGACAGTATTTCAGGATGACTCAATATAGTTGACATGATGATCTGAAACAGGAAAATACAGGAAGAAGGATTGGATTTACAGACGACTAAGAATGAAGATCATTTTGGTGCGCTCATTCACCACCTTTCATTAGTTCGCAATAAACGCTGCCTCATGGCTTATGTGTATGTCTAATATTGTGAGATAACTATGAGGAACTGACTTTTACCTGGTTTTCTGGGTTCGGGATAATTATATTTGCCAGTTAAATGTTTTTATATATAAACAAGCAGGTATAATCGAGCTGAGATTATAAGAAGTTTGATATGGAAGGTAGGATCTATGCTTCCACCAGAAATACAAGAGAAGCTTAGCAACTCAGAGGAAGAGTATTTTAAGAAGCATTCTGCATGTCTAAAAGCATACATGTCGAGACTGGAGTTGGAATTAACTGTGGTAATCTCTGACACCATTTTTCTATATATAATCTTTTTCCTTCTAAAATTTTAGGGAGCAAATTAATGAGAAATGTTTAAAATCTTTAACTTTCTCGTGTAATTTTCTTTAAAAGAAAAATTAAGGCTAATAAAGGGGTAATTACCCTCCTTCATTCTTGGCTTTTCGTTTACTCCTTTCCGATATATAATTGCAGTAGATAATTTAAAGCACTTGGTGGAAAATCATAAACCAATTTGAAACCACAACGCTTTAAAAACTTGTCGTGCTTTTTTGCTTCCAACTCAAATTCCTCGAACAGAAATTTCCAAGTTTATCATTGGCAGGATATGGTGCCACCTAAGGATCCATATATCCAAGTGAGGGTCCTCGATGATATCGGTGAAGGAATCATACTCAGTGATGATAAAACAGCAAACTTTGCACGCCATTCTATACATTTTCTCAAACGAACTGATGCTGAGCAATTCATCTCACGGGTGAGAATCCCTTCCAATTTTGTTTCGCAGTTCCTCTTATTGCTT

mRNA sequence

ATGTATGGAAGAAAAGCATGCCAGTTGGTGAAAGAGCTTGCAAACGGCGAGAAAGGTCAGCTAACGCACTTCAATAGCGATCTATTTGATCAGGCTATTTCAGAATGCGAGCAGCATCATCTTGAGCTTCAGTCCTTGATGAGGAAAATACAGGAAGAAGGATTGGATTTACAGACGACTAAGAATGAAGATCATTTTGGTGCGCTCATTCACCACCTTTCATTAGTTCGCAATAAACGCTGCCTCATGGCTTATGTGTATAATCGAGCTGAGATTATAAGAAGTTTGATATGGAAGGTAGGATCTATGCTTCCACCAGAAATACAAGAGAAGCTTAGCAACTCAGAGGAAGAGTATTTTAAGAAGCATTCTGCATGTCTAAAAGCATACATGTCGAGACTGGAGTTGGAATTAACTGTGGATATGGTGCCACCTAAGGATCCATATATCCAAGTGAGGGTCCTCGATGATATCGGTGAAGGAATCATACTCAGTGATGATAAAACAGCAAACTTTGCACGCCATTCTATACATTTTCTCAAACGAACTGATGCTGAGCAATTCATCTCACGGGTGAGAATCCCTTCCAATTTTGTTTCGCAGTTCCTCTTATTGCTT

Coding sequence (CDS)

ATGTATGGAAGAAAAGCATGCCAGTTGGTGAAAGAGCTTGCAAACGGCGAGAAAGGTCAGCTAACGCACTTCAATAGCGATCTATTTGATCAGGCTATTTCAGAATGCGAGCAGCATCATCTTGAGCTTCAGTCCTTGATGAGGAAAATACAGGAAGAAGGATTGGATTTACAGACGACTAAGAATGAAGATCATTTTGGTGCGCTCATTCACCACCTTTCATTAGTTCGCAATAAACGCTGCCTCATGGCTTATGTGTATAATCGAGCTGAGATTATAAGAAGTTTGATATGGAAGGTAGGATCTATGCTTCCACCAGAAATACAAGAGAAGCTTAGCAACTCAGAGGAAGAGTATTTTAAGAAGCATTCTGCATGTCTAAAAGCATACATGTCGAGACTGGAGTTGGAATTAACTGTGGATATGGTGCCACCTAAGGATCCATATATCCAAGTGAGGGTCCTCGATGATATCGGTGAAGGAATCATACTCAGTGATGATAAAACAGCAAACTTTGCACGCCATTCTATACATTTTCTCAAACGAACTGATGCTGAGCAATTCATCTCACGGGTGAGAATCCCTTCCAATTTTGTTTCGCAGTTCCTCTTATTGCTT

Protein sequence

MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTTKNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYFKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFLKRTDAEQFISRVRIPSNFVSQFLLLL

Homology

[Close]

BLAST of MS009421 vs. NCBI nr
Match: XP_022144990.1 (DNA replication complex GINS protein PSF1-like isoform X2 [Momordica charantia])

HSP 1 Score: 384.4 bits (986), Expect = 6.1e-103
Identity = 190/191 (99.48%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF 120
           KNEDHFGALIHHLSLVRNKRCLMAYVY+RAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF
Sbjct: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYDRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF 120

Query: 121 KKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL 180
           KKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL
Sbjct: 121 KKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL 180

Query: 181 KRTDAEQFISR 192
           KRTDAEQFISR
Sbjct: 181 KRTDAEQFISR 191

[Close]

BLAST of MS009421 vs. NCBI nr
Match: XP_022144973.1 (DNA replication complex GINS protein PSF1-like isoform X1 [Momordica charantia])

HSP 1 Score: 379.8 bits (974), Expect = 1.5e-101
Identity = 190/192 (98.96%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYV-YNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHLSLVRNKRCLMAYV Y+RAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLSLVRNKRCLMAYVRYDRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY 120

Query: 121 FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF 180
           FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF
Sbjct: 121 FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF 180

Query: 181 LKRTDAEQFISR 192
           LKRTDAEQFISR
Sbjct: 181 LKRTDAEQFISR 192

[Close]

BLAST of MS009421 vs. NCBI nr
Match: XP_022139358.1 (DNA replication complex GINS protein PSF1-like [Momordica charantia])

HSP 1 Score: 378.6 bits (971), Expect = 3.3e-101
Identity = 188/191 (98.43%), Postives = 188/191 (98.43%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKELANGEKGQLTHFNSDLFDQ ISECEQHHLELQSLMRKIQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQVISECEQHHLELQSLMRKIQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF 120
           KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF
Sbjct: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF 120

Query: 121 KKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL 180
           KKHSA LK YMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL
Sbjct: 121 KKHSARLKVYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL 180

Query: 181 KRTDAEQFISR 192
           KRTDAEQFISR
Sbjct: 181 KRTDAEQFISR 191

[Close]

BLAST of MS009421 vs. NCBI nr
Match: XP_004135842.1 (probable DNA replication complex GINS protein PSF1 [Cucumis sativus] >KGN45289.1 hypothetical protein Csa_016815 [Cucumis sativus])

HSP 1 Score: 355.5 bits (911), Expect = 3.0e-94
Identity = 174/192 (90.62%), Postives = 187/192 (97.40%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKELA+GEKGQLTHFNSDLF+Q ISEC+QHHLELQSL+RK+QEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWK-VGSMLPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHL+LVRNKRCLMAYV+NRAEIIRSL+WK VGSM+PPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLMWKVVGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF 180
           FKKHSA LK YMS+LEL+LTVDMVPPKDPYIQVRVLDDIGEGI+LSDDKTANFA HSIHF
Sbjct: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQFISR 192
           LKRTDAEQ+ISR
Sbjct: 181 LKRTDAEQYISR 192

[Close]

BLAST of MS009421 vs. NCBI nr
Match: XP_038897311.1 (probable DNA replication complex GINS protein PSF1 [Benincasa hispida])

HSP 1 Score: 354.0 bits (907), Expect = 8.8e-94
Identity = 173/192 (90.10%), Postives = 186/192 (96.88%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKEL +GEKGQLTHFNSDLF+Q ISEC+QHHLELQSL+RK+QEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELTSGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWK-VGSMLPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHL+LVRNKRCLMAYV+NRAEIIRSLIWK VGSM+PPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLIWKIVGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF 180
           FKKHSA +K YMS+LEL+LTVDMVPPKDPYIQVRVLDDIGEGI+LSDDKTANFA HSIHF
Sbjct: 121 FKKHSARVKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQFISR 192
           LKRTDAEQ+ISR
Sbjct: 181 LKRTDAEQYISR 192

[Close]

BLAST of MS009421 vs. ExPASy Swiss-Prot
Match: Q7ZT47 (DNA replication complex GINS protein PSF1 OS=Xenopus laevis OX=8355 GN=gins1 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 6.5e-23
Identity = 70/194 (36.08%), Postives = 104/194 (53.61%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEG-LDLQT 60
           M+  KA +L++EL     GQL  FN D   Q + E +  + + Q+ + + + EG  DL  
Sbjct: 1   MFCEKAIELIRELQRASDGQLPAFNEDGIRQILEEMKALYEQNQADVNEAKTEGRSDLIP 60

Query: 61  TKNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY 120
           T           H  L+RN+RC++AY+Y+R   IR+L W+ GS+LP  ++  +S  E ++
Sbjct: 61  T-------IKFRHCCLLRNRRCIVAYLYDRLLRIRALRWEYGSVLPSALRFHMSTEEMDW 120

Query: 121 FKKHSACLKAYMSRL----ELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARH 180
           F ++   L  YM  L     L++T DM PPK  YI+VR L D GE   + D  T    ++
Sbjct: 121 FNQYKRSLATYMRSLGGEEGLDITQDMKPPKSLYIEVRCLRDYGE-FEIDDGTTILLKKN 180

Query: 181 SIHFLKRTDAEQFI 190
           S HFL R   EQ I
Sbjct: 181 SQHFLPRWKCEQLI 186

[Close]

BLAST of MS009421 vs. ExPASy Swiss-Prot
Match: A4IFH4 (DNA replication complex GINS protein PSF1 OS=Bos taurus OX=9913 GN=GINS1 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.2e-22
Identity = 70/194 (36.08%), Postives = 104/194 (53.61%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGL-DLQT 60
           M+  KA +LV+EL    +G+L  FN D   Q + E +  + + QS + + +  G  DL  
Sbjct: 1   MFCEKAMELVRELHRAAEGRLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSSGRGDLIP 60

Query: 61  TKNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY 120
           T           H SL+RN+RC +AY+Y+R   IR+L W+ GS+LP  ++  +S  E ++
Sbjct: 61  T-------IKFRHCSLLRNQRCTVAYLYDRLLRIRALRWEYGSVLPSALRFHMSAEEMDW 120

Query: 121 FKKHSACLKAYMSRL----ELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARH 180
           F ++   L  YM  L     L++T DM PPK  YI+VR L D GE   + D  +    ++
Sbjct: 121 FNRYKKSLATYMRSLGGDEGLDITQDMKPPKSLYIEVRCLKDYGE-FEVEDGTSVLLKKN 180

Query: 181 SIHFLKRTDAEQFI 190
           S HFL R   EQ I
Sbjct: 181 SQHFLPRWKCEQLI 186

[Close]

BLAST of MS009421 vs. ExPASy Swiss-Prot
Match: Q14691 (DNA replication complex GINS protein PSF1 OS=Homo sapiens OX=9606 GN=GINS1 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.2e-22
Identity = 70/194 (36.08%), Postives = 103/194 (53.09%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEG-LDLQT 60
           M+  KA +L++EL    +GQL  FN D   Q + E +  + + QS + + +  G  DL  
Sbjct: 1   MFCEKAMELIRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSGGRSDLIP 60

Query: 61  TKNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY 120
           T           H SL+RN+RC +AY+Y+R   IR+L W+ GS+LP  ++  ++  E E+
Sbjct: 61  T-------IKFRHCSLLRNRRCTVAYLYDRLLRIRALRWEYGSVLPNALRFHMAAEEMEW 120

Query: 121 FKKHSACLKAYMSRL----ELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARH 180
           F  +   L  YM  L     L++T DM PPK  YI+VR L D GE   + D  +    ++
Sbjct: 121 FNNYKRSLATYMRSLGGDEGLDITQDMKPPKSLYIEVRCLKDYGE-FEVDDGTSVLLKKN 180

Query: 181 SIHFLKRTDAEQFI 190
           S HFL R   EQ I
Sbjct: 181 SQHFLPRWKCEQLI 186

[Close]

BLAST of MS009421 vs. ExPASy Swiss-Prot
Match: Q9CZ15 (DNA replication complex GINS protein PSF1 OS=Mus musculus OX=10090 GN=Gins1 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.2e-22
Identity = 70/194 (36.08%), Postives = 103/194 (53.09%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGL-DLQT 60
           M+  KA +LV+EL    +GQL  FN D   Q + E +  + + QS + + +  G  DL  
Sbjct: 1   MFCEKAMELVRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSAGRGDLIP 60

Query: 61  TKNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY 120
           T           H +L+RN+RC +AY+Y+R   IR+L W+ GS+LP  ++  +S  E E+
Sbjct: 61  T-------VKFRHCALLRNRRCTIAYLYDRLLRIRALRWEYGSVLPNSLRFHMSAEETEW 120

Query: 121 FKKHSACLKAYMSRL----ELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARH 180
           F  +   L  YM  L     L++T D+ PPK  YI+VR L D GE   + D  +    ++
Sbjct: 121 FNHYKKSLATYMRSLGGDEGLDITQDVKPPKSLYIEVRCLKDYGE-FEVDDGTSVLLKKN 180

Query: 181 SIHFLKRTDAEQFI 190
           S HFL R   EQ I
Sbjct: 181 SQHFLPRWKCEQLI 186

[Close]

BLAST of MS009421 vs. ExPASy Swiss-Prot
Match: Q54HR6 (Probable DNA replication complex GINS protein PSF1 OS=Dictyostelium discoideum OX=44689 GN=gins1 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.6e-21
Identity = 61/192 (31.77%), Postives = 108/192 (56.25%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           M+ + A +L+KEL   +   + H+N       I E    + +L   + + +E+       
Sbjct: 1   MFTKTAIELIKELRGTD--SIPHYNDTSIKATIDEMIALYEDLIKTITEHKEQ------- 60

Query: 61  KNEDHF--GALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVG-SMLPPEIQEKLSNSEE 120
           K E  +   A+  H S+ R+KRC++AY+  R   I+   W  G S+LP +++E+LS +E 
Sbjct: 61  KKEPFYLQHAITFHNSINRDKRCILAYLNERLNRIKEYRWSSGQSLLPDQLKERLSQNEI 120

Query: 121 EYFKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSI 180
           ++F ++   L  Y S++ L+LT+D  PPK+ YI+VRV+ ++GE ++L+   T N   ++ 
Sbjct: 121 QFFSEYDKILTEYNSKVGLDLTIDPQPPKELYIEVRVIKELGE-VVLNSGCTVNLNLNTT 180

Query: 181 HFLKRTDAEQFI 190
           HFLKR+D    +
Sbjct: 181 HFLKRSDITNLV 182

[Close]

BLAST of MS009421 vs. ExPASy TrEMBL
Match: A0A6J1CT73 (DNA replication complex GINS protein PSF1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111014510 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.0e-103
Identity = 190/191 (99.48%), Postives = 191/191 (100.00%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF 120
           KNEDHFGALIHHLSLVRNKRCLMAYVY+RAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF
Sbjct: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYDRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF 120

Query: 121 KKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL 180
           KKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL
Sbjct: 121 KKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL 180

Query: 181 KRTDAEQFISR 192
           KRTDAEQFISR
Sbjct: 181 KRTDAEQFISR 191

[Close]

BLAST of MS009421 vs. ExPASy TrEMBL
Match: A0A6J1CUP5 (DNA replication complex GINS protein PSF1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014510 PE=3 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.3e-102
Identity = 190/192 (98.96%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYV-YNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHLSLVRNKRCLMAYV Y+RAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLSLVRNKRCLMAYVRYDRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEY 120

Query: 121 FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF 180
           FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF
Sbjct: 121 FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF 180

Query: 181 LKRTDAEQFISR 192
           LKRTDAEQFISR
Sbjct: 181 LKRTDAEQFISR 192

[Close]

BLAST of MS009421 vs. ExPASy TrEMBL
Match: A0A6J1CFF5 (DNA replication complex GINS protein PSF1-like OS=Momordica charantia OX=3673 GN=LOC111010311 PE=3 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.6e-101
Identity = 188/191 (98.43%), Postives = 188/191 (98.43%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKELANGEKGQLTHFNSDLFDQ ISECEQHHLELQSLMRKIQEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQVISECEQHHLELQSLMRKIQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF 120
           KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF
Sbjct: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSMLPPEIQEKLSNSEEEYF 120

Query: 121 KKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL 180
           KKHSA LK YMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL
Sbjct: 121 KKHSARLKVYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHFL 180

Query: 181 KRTDAEQFISR 192
           KRTDAEQFISR
Sbjct: 181 KRTDAEQFISR 191

[Close]

BLAST of MS009421 vs. ExPASy TrEMBL
Match: A0A0A0K8U8 (Sld5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G433190 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 174/192 (90.62%), Postives = 187/192 (97.40%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLVKELA+GEKGQLTHFNSDLF+Q ISEC+QHHLELQSL+RK+QEEGLDLQTT
Sbjct: 1   MYGRKACQLVKELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWK-VGSMLPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHL+LVRNKRCLMAYV+NRAEIIRSL+WK VGSM+PPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAEIIRSLMWKVVGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF 180
           FKKHSA LK YMS+LEL+LTVDMVPPKDPYIQVRVLDDIGEGI+LSDDKTANFA HSIHF
Sbjct: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQFISR 192
           LKRTDAEQ+ISR
Sbjct: 181 LKRTDAEQYISR 192

[Close]

BLAST of MS009421 vs. ExPASy TrEMBL
Match: A0A1S3CDY2 (probable DNA replication complex GINS protein PSF1 OS=Cucumis melo OX=3656 GN=LOC103499784 PE=3 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 2.8e-93
Identity = 172/192 (89.58%), Postives = 185/192 (96.35%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRKACQLV ELA+GEKGQLTHFNSDLF+Q ISEC+QHHLELQSL+RK+QEEGLDLQTT
Sbjct: 1   MYGRKACQLVTELASGEKGQLTHFNSDLFEQVISECQQHHLELQSLIRKVQEEGLDLQTT 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWK-VGSMLPPEIQEKLSNSEEEY 120
           KNEDHFGALIHHL+LVRNKRCLMAYV+NRAE IRSLIWK +GSM+PPEIQEKLSNSEEEY
Sbjct: 61  KNEDHFGALIHHLALVRNKRCLMAYVHNRAETIRSLIWKLLGSMIPPEIQEKLSNSEEEY 120

Query: 121 FKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSIHF 180
           FKKHSA LK YMS+LEL+LTVDMVPPKDPYIQVRVLDDIGEGI+LSDDKTANFA HSIHF
Sbjct: 121 FKKHSARLKEYMSKLELDLTVDMVPPKDPYIQVRVLDDIGEGIVLSDDKTANFALHSIHF 180

Query: 181 LKRTDAEQFISR 192
           LKRTDAEQ+ISR
Sbjct: 181 LKRTDAEQYISR 192

[Close]

BLAST of MS009421 vs. TAIR 10
Match: AT1G80190.1 (partner of SLD five 1 )

HSP 1 Score: 274.2 bits (700), Expect = 8.3e-74
Identity = 132/194 (68.04%), Postives = 160/194 (82.47%), Query Frame = 0


Query: 1   MYGRKACQLVKELANGEKGQLTHFNSDLFDQAISECEQHHLELQSLMRKIQEEGLDLQTT 60
           MYGRK  QL+K+ A GEKGQL  FNS LFD+ I EC+Q+H  +QSL+RK+Q+EGLD+Q  
Sbjct: 1   MYGRKGYQLIKDFATGEKGQLKPFNSKLFDETIEECDQNHHLIQSLIRKMQQEGLDVQNN 60

Query: 61  KNEDHFGALIHHLSLVRNKRCLMAYVYNRAEIIRSLIWKVGSM---LPPEIQEKLSNSEE 120
           +N DH+GALIHHL+L+RNKRCLMAYVYNRAEI+R L W+VG     LP EIQEKL+  E+
Sbjct: 61  RNADHYGALIHHLALIRNKRCLMAYVYNRAEIVRDLAWRVGLELLDLPSEIQEKLTTLEK 120

Query: 121 EYFKKHSACLKAYMSRLELELTVDMVPPKDPYIQVRVLDDIGEGIILSDDKTANFARHSI 180
           EYFK HS  LK+YM ++ +EL VDMVPPKDPYI+VR+LDDI EGI+LS DKT NFARHS+
Sbjct: 121 EYFKNHSVALKSYMGKVGIELNVDMVPPKDPYIKVRILDDIDEGIVLS-DKTTNFARHSM 180

Query: 181 HFLKRTDAEQFISR 192
           HFLKRTDAE +I+R
Sbjct: 181 HFLKRTDAEPYIAR 193

The following BLAST results are available for this feature:

BLAST of MS009421 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144990.1	6.1e-103	99.48	DNA replication complex GINS protein PSF1-like isoform X2 [Momordica charantia]	[more]
XP_022144973.1	1.5e-101	98.96	DNA replication complex GINS protein PSF1-like isoform X1 [Momordica charantia]	[more]
XP_022139358.1	3.3e-101	98.43	DNA replication complex GINS protein PSF1-like [Momordica charantia]	[more]
XP_004135842.1	3.0e-94	90.63	probable DNA replication complex GINS protein PSF1 [Cucumis sativus] >KGN45289.1...	[more]
XP_038897311.1	8.8e-94	90.10	probable DNA replication complex GINS protein PSF1 [Benincasa hispida]	[more]

BLAST of MS009421 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7ZT47	6.5e-23	36.08	DNA replication complex GINS protein PSF1 OS=Xenopus laevis OX=8355 GN=gins1 PE=...	[more]
A4IFH4	3.2e-22	36.08	DNA replication complex GINS protein PSF1 OS=Bos taurus OX=9913 GN=GINS1 PE=2 SV...	[more]
Q14691	4.2e-22	36.08	DNA replication complex GINS protein PSF1 OS=Homo sapiens OX=9606 GN=GINS1 PE=1 ...	[more]
Q9CZ15	4.2e-22	36.08	DNA replication complex GINS protein PSF1 OS=Mus musculus OX=10090 GN=Gins1 PE=1...	[more]
Q54HR6	4.6e-21	31.77	Probable DNA replication complex GINS protein PSF1 OS=Dictyostelium discoideum O...	[more]

BLAST of MS009421 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CT73	3.0e-103	99.48	DNA replication complex GINS protein PSF1-like isoform X2 OS=Momordica charantia...	[more]
A0A6J1CUP5	7.3e-102	98.96	DNA replication complex GINS protein PSF1-like isoform X1 OS=Momordica charantia...	[more]
A0A6J1CFF5	1.6e-101	98.43	DNA replication complex GINS protein PSF1-like OS=Momordica charantia OX=3673 GN...	[more]
A0A0A0K8U8	1.5e-94	90.63	Sld5 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G433190 PE=3 S...	[more]
A0A1S3CDY2	2.8e-93	89.58	probable DNA replication complex GINS protein PSF1 OS=Cucumis melo OX=3656 GN=LO...	[more]

BLAST of MS009421 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80190.1	8.3e-74	68.04	partner of SLD five 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 33..63
None	No IPR available	GENE3D	1.20.58.1030		coord: 1..134 e-value: 4.2E-40 score: 138.9
IPR021151	GINS subunit, domain A	PFAM	PF05916	Sld5	coord: 47..116 e-value: 5.8E-7 score: 30.0
IPR005339	GINS complex, subunit Psf1	PANTHER	PTHR12914	PARTNER OF SLD5	coord: 1..192
IPR005339	GINS complex, subunit Psf1	CDD	cd11710	GINS_A_psf1	coord: 3..136 e-value: 3.03386E-47 score: 149.713
IPR036224	GINS, helical bundle-like domain superfamily	SUPERFAMILY	158573	GINS helical bundle-like	coord: 1..146

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009421.1	MS009421.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006260	DNA replication
cellular_component	GO:0000811	GINS complex