MS009409 (gene) Bitter gourd (TR) v1

Overview


Name	MS009409
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Cytochrome P450 family protein
Location	scaffold813: 1132713 .. 1133227 (-)
RNA-Seq Expression	MS009409
Synteny	MS009409

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGTCCATTTTGAATTCTTCCAATTTGGTTTTCGCCACCGCCATGGTCGTCTCCAGCACGGCGGTGCTTCTCTCCTTTTTCCGGCATAATAAGCCCGACATCTCGCCGCCGCCGCCGCCACTCCACGACGGCCGCACTTCCCGCCAACCCGATGTTCAGATTCTTCGCCCATGTCTATCTTCAGGTACAATTAGGCAACAAAAAAGGGAAAAGATTCAATTTTTTGGGGAAAGTTTTGAATTTTTTTTTGTTGGGGGTTTGTTGCAGGGGGGAATAAAGAGAGAAAGAAGAAGAAAGTACAGTTTGCAGAGAATGTGAAGAAGAAGAAGAAGGTGACAGAGGGAGAGATTATTAATGTCGAGGAGTTTAAGAAAAAAACCGACAGAAAATTGAAGAGAATACGATGCAGAAATGATTTTGCCGGAATTCAAGTCCGAGGAATGCCGGAAAATCGAGTGGCTCTCTATAATGGAATTCTCAGAGATCGATCTCAGAGGCTGGGGTTTTCATAT

mRNA sequence

ATGTCGTCCATTTTGAATTCTTCCAATTTGGTTTTCGCCACCGCCATGGTCGTCTCCAGCACGGCGGTGCTTCTCTCCTTTTTCCGGCATAATAAGCCCGACATCTCGCCGCCGCCGCCGCCACTCCACGACGGCCGCACTTCCCGCCAACCCGATGTTCAGATTCTTCGCCCATGTCTATCTTCAGGGGGGAATAAAGAGAGAAAGAAGAAGAAAGTACAGTTTGCAGAGAATGTGAAGAAGAAGAAGAAGGTGACAGAGGGAGAGATTATTAATGTCGAGGAGTTTAAGAAAAAAACCGACAGAAAATTGAAGAGAATACGATGCAGAAATGATTTTGCCGGAATTCAAGTCCGAGGAATGCCGGAAAATCGAGTGGCTCTCTATAATGGAATTCTCAGAGATCGATCTCAGAGGCTGGGGTTTTCATAT

Coding sequence (CDS)

ATGTCGTCCATTTTGAATTCTTCCAATTTGGTTTTCGCCACCGCCATGGTCGTCTCCAGCACGGCGGTGCTTCTCTCCTTTTTCCGGCATAATAAGCCCGACATCTCGCCGCCGCCGCCGCCACTCCACGACGGCCGCACTTCCCGCCAACCCGATGTTCAGATTCTTCGCCCATGTCTATCTTCAGGGGGGAATAAAGAGAGAAAGAAGAAGAAAGTACAGTTTGCAGAGAATGTGAAGAAGAAGAAGAAGGTGACAGAGGGAGAGATTATTAATGTCGAGGAGTTTAAGAAAAAAACCGACAGAAAATTGAAGAGAATACGATGCAGAAATGATTTTGCCGGAATTCAAGTCCGAGGAATGCCGGAAAATCGAGTGGCTCTCTATAATGGAATTCTCAGAGATCGATCTCAGAGGCTGGGGTTTTCATAT

Protein sequence

MSSILNSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCLSSGGNKERKKKKVQFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIRCRNDFAGIQVRGMPENRVALYNGILRDRSQRLGFSY

Homology

[Close]

BLAST of MS009409 vs. NCBI nr
Match: XP_038900153.1 (uncharacterized protein LOC120087277 [Benincasa hispida])

HSP 1 Score: 156.4 bits (394), Expect = 1.9e-34
Identity = 94/152 (61.84%), Postives = 107/152 (70.39%), Query Frame = 0


Query: 1   MSSILNSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCL 60
           MSSILNSSNLVFAT MVVSSTAV+L+F R NKPDISPPPP L DG TSRQ +  ILRPCL
Sbjct: 1   MSSILNSSNLVFATVMVVSSTAVILAFLRQNKPDISPPPPQLSDGTTSRQSESPILRPCL 60

Query: 61  SSGGNKERKKKKVQFAENVKKKK--------KVTEGEIINVEEFKKKTDRKLKRIRCRND 120
           SSGG KERKKKKVQFAENVKK K        K      ++       +   LK+++CRN 
Sbjct: 61  SSGGKKERKKKKVQFAENVKKMKCTDHDHIRKKERSSALSASASSASSRLLLKKVQCRN- 120

Query: 121 FAGIQVRGMPENRVALYNGILRDRSQRLGFSY 145
            AG     +P+NR+ALYN ILRDRS R+ FSY
Sbjct: 121 -AG--RNQIPQNRLALYNAILRDRSHRIQFSY 148

[Close]

BLAST of MS009409 vs. NCBI nr
Match: KAG6593394.1 (hypothetical protein SDJN03_12870, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025741.1 hypothetical protein SDJN02_12239, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 151.8 bits (382), Expect = 4.6e-33
Identity = 96/146 (65.75%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 1   MSSILNSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCL 60
           M SILNSSNLVFATAMV SST ++L++FRHNK           DG  SRQ DV+ILRPCL
Sbjct: 1   MPSILNSSNLVFATAMVASSTLMILAYFRHNK----------GDGAASRQADVRILRPCL 60

Query: 61  SSGGNKERKK--KKVQFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIRCRNDFAGIQV 120
            SGGNK+RKK  KKVQFAENV  KKK TE E    E  KK   RKLK+ RCR+ F G Q 
Sbjct: 61  CSGGNKDRKKKQKKVQFAENV--KKKATEAE----EMKKKIMARKLKKNRCRSSFQGSQ- 120

Query: 121 RGMPENRVALYNGILRDRSQRLGFSY 145
             MPENRVALYNGILRDRS R+GFSY
Sbjct: 121 --MPENRVALYNGILRDRSNRMGFSY 127

[Close]

BLAST of MS009409 vs. NCBI nr
Match: XP_023004360.1 (uncharacterized protein LOC111497701 [Cucurbita maxima])

HSP 1 Score: 149.4 bits (376), Expect = 2.3e-32
Identity = 94/146 (64.38%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 1   MSSILNSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCL 60
           M SILNSSNLVFATAMV SST ++L++FRHNK           D   +RQ DV+ILRPCL
Sbjct: 1   MPSILNSSNLVFATAMVASSTLMILAYFRHNK----------GDSAATRQADVRILRPCL 60

Query: 61  SSGGNKERKK--KKVQFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIRCRNDFAGIQV 120
            SGGNK+RKK  KKVQFAENV  KKK TE E    E  +K T RKLK+ RCR+ F G Q 
Sbjct: 61  CSGGNKDRKKKQKKVQFAENV--KKKATEAE----EMKRKITARKLKKNRCRSSFQGSQ- 120

Query: 121 RGMPENRVALYNGILRDRSQRLGFSY 145
             MPENRVALYNGILRDRS R+GFSY
Sbjct: 121 --MPENRVALYNGILRDRSNRMGFSY 127

[Close]

BLAST of MS009409 vs. NCBI nr
Match: XP_023514528.1 (uncharacterized protein LOC111778785 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 133.3 bits (334), Expect = 1.7e-27
Identity = 83/131 (63.36%), Postives = 91/131 (69.47%), Query Frame = 0


Query: 16  MVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCLSSGGNKERKK--KKV 75
           MV SST ++L++FRHNK           DG  SRQ DV+ILRPCL SGGNK+RKK  KKV
Sbjct: 1   MVASSTLMILAYFRHNK----------GDGAASRQSDVRILRPCLCSGGNKDRKKKQKKV 60

Query: 76  QFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIRCRNDFAGIQVRGMPENRVALYNGIL 135
           QFAENV  KKK TE E    E  KK T RKLK+ RCR  F G Q   MPENRVALYNGIL
Sbjct: 61  QFAENV--KKKATEAE----ETKKKMTARKLKKNRCRGSFQGSQ---MPENRVALYNGIL 112

Query: 136 RDRSQRLGFSY 145
           RDRS R+GFSY
Sbjct: 121 RDRSNRMGFSY 112

[Close]

BLAST of MS009409 vs. NCBI nr
Match: XP_022960430.1 (uncharacterized protein LOC111461163 [Cucurbita moschata])

HSP 1 Score: 130.2 bits (326), Expect = 1.4e-26
Identity = 82/131 (62.60%), Postives = 91/131 (69.47%), Query Frame = 0


Query: 16  MVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCLSSGGNKERKK--KKV 75
           MV SST ++L++FRHNK           DG  SRQ DV+ILRPCL SGGNK+RKK  KKV
Sbjct: 1   MVASSTLMILAYFRHNK----------GDGAASRQADVRILRPCLCSGGNKDRKKKQKKV 60

Query: 76  QFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIRCRNDFAGIQVRGMPENRVALYNGIL 135
           QFAENV  KKK TE E    E  KK   RKLK+ RCR+ F G Q   MPENRVALYNGIL
Sbjct: 61  QFAENV--KKKATEAE----EMKKKIMARKLKKNRCRSSFQGSQ---MPENRVALYNGIL 112

Query: 136 RDRSQRLGFSY 145
           RDRS R+GFSY
Sbjct: 121 RDRSNRMGFSY 112

[Close]

BLAST of MS009409 vs. ExPASy TrEMBL
Match: A0A6J1KUC7 (uncharacterized protein LOC111497701 OS=Cucurbita maxima OX=3661 GN=LOC111497701 PE=4 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 1.1e-32
Identity = 94/146 (64.38%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 1   MSSILNSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCL 60
           M SILNSSNLVFATAMV SST ++L++FRHNK           D   +RQ DV+ILRPCL
Sbjct: 1   MPSILNSSNLVFATAMVASSTLMILAYFRHNK----------GDSAATRQADVRILRPCL 60

Query: 61  SSGGNKERKK--KKVQFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIRCRNDFAGIQV 120
            SGGNK+RKK  KKVQFAENV  KKK TE E    E  +K T RKLK+ RCR+ F G Q 
Sbjct: 61  CSGGNKDRKKKQKKVQFAENV--KKKATEAE----EMKRKITARKLKKNRCRSSFQGSQ- 120

Query: 121 RGMPENRVALYNGILRDRSQRLGFSY 145
             MPENRVALYNGILRDRS R+GFSY
Sbjct: 121 --MPENRVALYNGILRDRSNRMGFSY 127

[Close]

BLAST of MS009409 vs. ExPASy TrEMBL
Match: A0A6J1H919 (uncharacterized protein LOC111461163 OS=Cucurbita moschata OX=3662 GN=LOC111461163 PE=4 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 7.0e-27
Identity = 82/131 (62.60%), Postives = 91/131 (69.47%), Query Frame = 0


Query: 16  MVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCLSSGGNKERKK--KKV 75
           MV SST ++L++FRHNK           DG  SRQ DV+ILRPCL SGGNK+RKK  KKV
Sbjct: 1   MVASSTLMILAYFRHNK----------GDGAASRQADVRILRPCLCSGGNKDRKKKQKKV 60

Query: 76  QFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIRCRNDFAGIQVRGMPENRVALYNGIL 135
           QFAENV  KKK TE E    E  KK   RKLK+ RCR+ F G Q   MPENRVALYNGIL
Sbjct: 61  QFAENV--KKKATEAE----EMKKKIMARKLKKNRCRSSFQGSQ---MPENRVALYNGIL 112

Query: 136 RDRSQRLGFSY 145
           RDRS R+GFSY
Sbjct: 121 RDRSNRMGFSY 112

[Close]

BLAST of MS009409 vs. ExPASy TrEMBL
Match: A0A0A0KA62 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G432590 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 5.6e-24
Identity = 81/167 (48.50%), Postives = 107/167 (64.07%), Query Frame = 0


Query: 1   MSSILNSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGR-TSRQPDVQILRPC 60
           MSS+LNSSNLVFAT MVVSSTA++L+  R NKPD+S PPP    G  TS +  +QILRPC
Sbjct: 1   MSSLLNSSNLVFATVMVVSSTALILALLRQNKPDLSSPPPSQLSGETTSPKSQLQILRPC 60

Query: 61  LSSGGNKERKKKKVQFAEN--------VKKK--KKVTEGEIINVEEFKKKTDRKLKRIRC 120
           LSSGG K +KKKKVQFAEN        VKKK  K++   +I+ +   K +  + +K+++C
Sbjct: 61  LSSGGKKGKKKKKVQFAENNNNNTNSDVKKKGIKEIQREKIMLMMMKKNERLKLVKKVQC 120

Query: 121 RND-----------FAGIQVRGMPENRVALYNGILRDR-SQRLGFSY 145
           RN+            +      +P+NR+ LYN ILRDR + R+ FSY
Sbjct: 121 RNNNNAKSSSSSSSSSSSSNHRIPQNRLTLYNAILRDRCAHRIQFSY 167

[Close]

BLAST of MS009409 vs. ExPASy TrEMBL
Match: A0A6P3YZF0 (uncharacterized protein LOC107407483 OS=Ziziphus jujuba OX=326968 GN=LOC107407483 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 9.2e-19
Identity = 74/150 (49.33%), Postives = 88/150 (58.67%), Query Frame = 0


Query: 1   MSSILNSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCL 60
           MSS+L+S  LV ATAM VSST + L+F RH    +            S+QP   +LR CL
Sbjct: 1   MSSVLSSQGLVLATAMAVSSTVLFLAFSRHKTTFLPTQFSGTGSYNDSQQPKKHVLRSCL 60

Query: 61  SSGGN---KERKKKKVQFAENVKKKKKVTEGEIINVEEFK---KKTDRKLKRIRCRNDFA 120
            SG +   K+RKKKKVQFAENVK+          N EEFK   KKT +  K    RN  +
Sbjct: 61  CSGDSRKKKQRKKKKVQFAENVKEPSG-------NGEEFKKQHKKTSKSEKSSNSRNQMS 120

Query: 121 GIQVRGMPENRVALYNGILRDRSQRLGFSY 145
           GI  RGMP NR+ALYNGILRDR  R+  SY
Sbjct: 121 GI--RGMPANRIALYNGILRDRVHRMECSY 141

[Close]

BLAST of MS009409 vs. ExPASy TrEMBL
Match: A0A2N9J8B3 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60665 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 1.5e-16
Identity = 71/148 (47.97%), Postives = 93/148 (62.84%), Query Frame = 0


Query: 1   MSSILNSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCL 60
           MSSI  S  LV ATAMVVSST ++L++ R      + PPP L   + S+QP+ +ILR CL
Sbjct: 1   MSSI--SQGLVLATAMVVSSTVLILTYRRPK----TFPPPQLSQNQNSQQPENKILRSCL 60

Query: 61  SSGGNK--ERKKKKVQFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIR--CRNDFAGI 120
            S   K   +KKKKVQFAENVK+          N EE+ +K    L ++   CRN+ +  
Sbjct: 61  HSEEKKRERKKKKKVQFAENVKEPSG-------NGEEY-RKVHMNLSKVERSCRNEIS-- 120

Query: 121 QVRGMPENRVALYNGILRDRSQRLGFSY 145
            +RGMPENR+ALYNGIL+DR QR+  S+
Sbjct: 121 TIRGMPENRIALYNGILKDRVQRMQCSF 132

[Close]

BLAST of MS009409 vs. TAIR 10
Match: AT1G52565.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G15760.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 74.3 bits (181), Expect = 8.8e-14
Identity = 68/158 (43.04%), Postives = 88/158 (55.70%), Query Frame = 0


Query: 1   MSSILNSSN-LVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPC 60
           M+ IL+ S+ +V ATAMV SSTA+ L+  R                +TS   D QILR C
Sbjct: 1   MTCILSCSHGVVIATAMVFSSTALFLAISRQ-----------FSGNQTS---DQQILRSC 60

Query: 61  LSSGGNKER---KKKKVQFAENVKKKKKVTEGEIINVEEFKKKTDRKL---KRIR----- 120
           LSS   K++   KKKKV+FAENVK+ K        N EE++KK+ R++   + I+     
Sbjct: 61  LSSEEKKKQRKNKKKKVKFAENVKETKG-------NGEEYRKKSLRRIVPEQMIKPEKTG 120

Query: 121 --CRNDFAGIQVRGMPENRVALYNGILRDRSQRLGFSY 145
             CRND        MP NR+ALYNGILRDR  R+  SY
Sbjct: 121 SVCRND--------MPANRIALYNGILRDRDHRIQCSY 129

[Close]

BLAST of MS009409 vs. TAIR 10
Match: AT3G15760.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 10 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G52565.1); Has 42 Blast hits to 42 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 42; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 59.7 bits (143), Expect = 2.2e-09
Identity = 54/141 (38.30%), Postives = 74/141 (52.48%), Query Frame = 0


Query: 6   NSSNLVFATAMVVSSTAVLLSFFRHNKPDISPPPPPLHDGRTSRQPDVQILRPCLSSGGN 65
           ++  +V ATAMV SSTA+ L+  R    +   P       +   Q    ILR CLSS   
Sbjct: 5   SNGTVVIATAMVCSSTALFLAMARQFHGNHQNP-------KVLDQTLRPILRSCLSSEET 64

Query: 66  KE--RKKKKVQFAENVKKKKKVTEGEIINVEEFKKKTDRKLKRIRCRNDFAGIQVRGMPE 125
           K+  +K KKV+FA+NVK  K    GE     E  +K+  K    +     +  ++  MP 
Sbjct: 65  KKQGKKIKKVRFADNVKDTK--GNGEEYRRRELNRKSVPK-PVTKPGKTGSMCRISTMPA 124

Query: 126 NRVALYNGILRDRSQRLGFSY 145
           NR+ALYNGILRDR  R+ +SY
Sbjct: 125 NRMALYNGILRDRDHRVQYSY 135

The following BLAST results are available for this feature:

BLAST of MS009409 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900153.1	1.9e-34	61.84	uncharacterized protein LOC120087277 [Benincasa hispida]	[more]
KAG6593394.1	4.6e-33	65.75	hypothetical protein SDJN03_12870, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023004360.1	2.3e-32	64.38	uncharacterized protein LOC111497701 [Cucurbita maxima]	[more]
XP_023514528.1	1.7e-27	63.36	uncharacterized protein LOC111778785 [Cucurbita pepo subsp. pepo]	[more]
XP_022960430.1	1.4e-26	62.60	uncharacterized protein LOC111461163 [Cucurbita moschata]	[more]

BLAST of MS009409 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS009409 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KUC7	1.1e-32	64.38	uncharacterized protein LOC111497701 OS=Cucurbita maxima OX=3661 GN=LOC111497701...	[more]
A0A6J1H919	7.0e-27	62.60	uncharacterized protein LOC111461163 OS=Cucurbita moschata OX=3662 GN=LOC1114611...	[more]
A0A0A0KA62	5.6e-24	48.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G432590 PE=4 SV=1	[more]
A0A6P3YZF0	9.2e-19	49.33	uncharacterized protein LOC107407483 OS=Ziziphus jujuba OX=326968 GN=LOC10740748...	[more]
A0A2N9J8B3	1.5e-16	47.97	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS60665 PE=4 SV=1	[more]

BLAST of MS009409 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52565.1	8.8e-14	43.04	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G15760.1	2.2e-09	38.30	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33564	TRANSMEMBRANE PROTEIN	coord: 1..144
None	No IPR available	PANTHER	PTHR33564:SF15	PROTEIN, PUTATIVE-RELATED	coord: 1..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009409.1	MS009409.1	mRNA