MS009397 (gene) Bitter gourd (TR) v1

Overview
NameMS009397
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCytochrome b6
Locationscaffold813: 1042838 .. 1043059 (+)
RNA-Seq ExpressionMS009397
SyntenyMS009397
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGTTACAGGTGTGATTCTGGTTGTATTGACCGTATCCTTTGGTGTAATTGGTTATTCCTTACCTTGGGACCAAATTGGTTATAGAGCAGTCAAAATTGTAATAGGTGTACCGAAAGCTATTCCAATAATAGGGTCACATTTGATAGAATTATTATGCGGAAGTGCTAGTGTGAGACAATCTACCTTGACTCGTTTTTATAGTTTAAACACTTTAACTTTT

mRNA sequence

TGGGTTACAGGTGTGATTCTGGTTGTATTGACCGTATCCTTTGGTGTAATTGGTTATTCCTTACCTTGGGACCAAATTGGTTATAGAGCAGTCAAAATTGTAATAGGTGTACCGAAAGCTATTCCAATAATAGGGTCACATTTGATAGAATTATTATGCGGAAGTGCTAGTGTGAGACAATCTACCTTGACTCGTTTTTATAGTTTAAACACTTTAACTTTT

Coding sequence (CDS)

TGGGTTACAGGTGTGATTCTGGTTGTATTGACCGTATCCTTTGGTGTAATTGGTTATTCCTTACCTTGGGACCAAATTGGTTATAGAGCAGTCAAAATTGTAATAGGTGTACCGAAAGCTATTCCAATAATAGGGTCACATTTGATAGAATTATTATGCGGAAGTGCTAGTGTGAGACAATCTACCTTGACTCGTTTTTATAGTTTAAACACTTTAACTTTT

Protein sequence

WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQSTLTRFYSLNTLTF
Homology
BLAST of MS009397 vs. NCBI nr
Match: PNT67432.1 (hypothetical protein BRADI_3g27315v3 [Brachypodium distachyon])

HSP 1 Score: 118.2 bits (295), Expect = 2.9e-23
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGV+L VLT SFGV GYSLPWDQIGY AVKIV GVP AIP+IGS L+ELLCGSASV Q
Sbjct: 65  WVTGVVLAVLTASFGVTGYSLPWDQIGYWAVKIVTGVPDAIPVIGSPLVELLCGSASVGQ 124

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 125 STLTRFYSLHT 135

BLAST of MS009397 vs. NCBI nr
Match: YP_009490514.1 (cytochrome b6 [Cibotium barometz] >AWH62732.1 cytochrome b6 [Cibotium barometz])

HSP 1 Score: 117.9 bits (294), Expect = 3.8e-23
Identity = 60/71 (84.51%), Postives = 64/71 (90.14%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGYRAVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 120 WVTGVILAVLTVSFGVTGYSLPWDQIGYRAVKIVTGVPEAIPLIGSSLVELLRGSVSVGQ 179

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 180 STLTRFYSLHT 190

BLAST of MS009397 vs. NCBI nr
Match: YP_009502347.1 (cytochrome b6 [Alsophila podophylla] >YP_009679346.1 cytochrome b6 [Alsophila gigantea] >AWV63397.1 cytochrome b6 [Alsophila podophylla] >QDP70896.1 cytochrome b6 [Alsophila gigantea] >QXU59522.1 cytochrome b6 [Alsophila metteniana])

HSP 1 Score: 117.9 bits (294), Expect = 3.8e-23
Identity = 60/71 (84.51%), Postives = 64/71 (90.14%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGYRAVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYRAVKIVTGVPEAIPLIGSSLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. NCBI nr
Match: QZN05751.1 (cytochrome b6 [Alsophila denticulata])

HSP 1 Score: 117.9 bits (294), Expect = 3.8e-23
Identity = 60/71 (84.51%), Postives = 64/71 (90.14%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGYRAVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 109 WVTGVILAVLTVSFGVTGYSLPWDQIGYRAVKIVTGVPEAIPLIGSSLVELLRGSVSVGQ 168

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 169 STLTRFYSLHT 179

BLAST of MS009397 vs. NCBI nr
Match: YP_002970674.1 (cytochrome b6 [Alsophila spinulosa] >YP_009679435.1 cytochrome b6 [Alsophila costularis] >ACK77166.1 cytochrome b6 [Alsophila spinulosa] >QDP70978.1 cytochrome b6 [Alsophila costularis] >QUJ10087.1 cytochrome b6 [Alsophila latebrosa])

HSP 1 Score: 117.9 bits (294), Expect = 3.8e-23
Identity = 60/71 (84.51%), Postives = 64/71 (90.14%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGYRAVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYRAVKIVTGVPEAIPLIGSSLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. ExPASy Swiss-Prot
Match: P06248 (Cytochrome b6 OS=Marchantia polymorpha OX=3197 GN=petB PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 3.2e-25
Identity = 60/71 (84.51%), Postives = 63/71 (88.73%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGY AVKIV GVP+AIPIIGS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYWAVKIVTGVPEAIPIIGSPLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. ExPASy Swiss-Prot
Match: Q3BAK7 (Cytochrome b6 OS=Phalaenopsis aphrodite subsp. formosana OX=308872 GN=petB PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 4.2e-25
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGV+L VLT SFGV GYSLPWDQIGY AVKIV GVP+AIPIIGS L+ELL GSASV Q
Sbjct: 118 WVTGVVLAVLTASFGVTGYSLPWDQIGYWAVKIVTGVPEAIPIIGSPLVELLRGSASVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. ExPASy Swiss-Prot
Match: Q85X07 (Cytochrome b6 OS=Pinus koraiensis OX=88728 GN=petB PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 4.2e-25
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGY AVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYWAVKIVTGVPEAIPVIGSPLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. ExPASy Swiss-Prot
Match: P41628 (Cytochrome b6 OS=Pinus thunbergii OX=3350 GN=petB PE=3 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 4.2e-25
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGY AVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYWAVKIVTGVPEAIPVIGSPLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. ExPASy Swiss-Prot
Match: Q85A24 (Cytochrome b6 OS=Anthoceros angustus OX=48387 GN=petB PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 5.5e-25
Identity = 58/71 (81.69%), Postives = 63/71 (88.73%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGY AVKIV GVP+AIP++GS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYWAVKIVTGVPEAIPLVGSSLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. ExPASy TrEMBL
Match: A0A2K2CZH6 (CYTB_NTER domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_3g27315v3 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.4e-23
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGV+L VLT SFGV GYSLPWDQIGY AVKIV GVP AIP+IGS L+ELLCGSASV Q
Sbjct: 65  WVTGVVLAVLTASFGVTGYSLPWDQIGYWAVKIVTGVPDAIPVIGSPLVELLCGSASVGQ 124

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 125 STLTRFYSLHT 135

BLAST of MS009397 vs. ExPASy TrEMBL
Match: A0A287WHJ4 (CYTB_NTER domain-containing protein OS=Hordeum vulgare subsp. vulgare OX=112509 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.4e-23
Identity = 59/71 (83.10%), Postives = 63/71 (88.73%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGV+L VLT SFGV GYSLPWDQIGY AVKIV GVP AIP+IGS L+ELLCGSASV Q
Sbjct: 75  WVTGVVLAVLTASFGVTGYSLPWDQIGYWAVKIVTGVPDAIPVIGSPLVELLCGSASVGQ 134

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 135 STLTRFYSLHT 145

BLAST of MS009397 vs. ExPASy TrEMBL
Match: A0A7L8XKV7 (Cytochrome b6 OS=Sphaeropteris brunoniana OX=204593 GN=petB PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.9e-23
Identity = 60/71 (84.51%), Postives = 64/71 (90.14%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGYRAVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYRAVKIVTGVPEAIPLIGSSLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. ExPASy TrEMBL
Match: A0A344AIQ6 (Cytochrome b6 OS=Alsophila podophylla OX=204585 GN=petB PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.9e-23
Identity = 60/71 (84.51%), Postives = 64/71 (90.14%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGYRAVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYRAVKIVTGVPEAIPLIGSSLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. ExPASy TrEMBL
Match: C5HY84 (Cytochrome b6 OS=Alsophila spinulosa OX=204586 GN=petB PE=3 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.9e-23
Identity = 60/71 (84.51%), Postives = 64/71 (90.14%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGVIL VLTVSFGV GYSLPWDQIGYRAVKIV GVP+AIP+IGS L+ELL GS SV Q
Sbjct: 118 WVTGVILAVLTVSFGVTGYSLPWDQIGYRAVKIVTGVPEAIPLIGSSLVELLRGSVSVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. TAIR 10
Match: ATCG00720.1 (photosynthetic electron transfer B )

HSP 1 Score: 112.5 bits (280), Expect = 1.5e-25
Identity = 58/71 (81.69%), Postives = 62/71 (87.32%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           WVTGV+L VLT SFGV GYSLPWDQIGY AVKIV GVP AIP+IGS L+ELL GSASV Q
Sbjct: 118 WVTGVVLGVLTASFGVTGYSLPWDQIGYWAVKIVTGVPDAIPVIGSPLVELLRGSASVGQ 177

Query: 61  STLTRFYSLNT 72
           STLTRFYSL+T
Sbjct: 178 STLTRFYSLHT 188

BLAST of MS009397 vs. TAIR 10
Match: AT2G07727.1 (Di-haem cytochrome, transmembrane;Cytochrome b/b6, C-terminal )

HSP 1 Score: 57.0 bits (136), Expect = 7.5e-09
Identity = 26/72 (36.11%), Postives = 42/72 (58.33%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           W  GV++ +L +    IGY LPW Q+ +    ++  +  AIP++G  ++  L G  SV  
Sbjct: 120 WCLGVVIFLLMIVTAFIGYVLPWGQMSFWGATVITSLASAIPVVGDTIVTWLWGGFSVDN 179

Query: 61  STLTRFYSLNTL 73
           +TL RF+SL+ L
Sbjct: 180 ATLNRFFSLHHL 191

BLAST of MS009397 vs. TAIR 10
Match: ATMG00220.1 (apocytochrome b )

HSP 1 Score: 57.0 bits (136), Expect = 7.5e-09
Identity = 26/72 (36.11%), Postives = 42/72 (58.33%), Query Frame = 0

Query: 1   WVTGVILVVLTVSFGVIGYSLPWDQIGYRAVKIVIGVPKAIPIIGSHLIELLCGSASVRQ 60
           W  GV++ +L +    IGY LPW Q+ +    ++  +  AIP++G  ++  L G  SV  
Sbjct: 120 WCLGVVIFLLMIVTAFIGYVLPWGQMSFWGATVITSLASAIPVVGDTIVTWLWGGFSVDN 179

Query: 61  STLTRFYSLNTL 73
           +TL RF+SL+ L
Sbjct: 180 ATLNRFFSLHHL 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PNT67432.12.9e-2383.10hypothetical protein BRADI_3g27315v3 [Brachypodium distachyon][more]
YP_009490514.13.8e-2384.51cytochrome b6 [Cibotium barometz] >AWH62732.1 cytochrome b6 [Cibotium barometz][more]
YP_009502347.13.8e-2384.51cytochrome b6 [Alsophila podophylla] >YP_009679346.1 cytochrome b6 [Alsophila gi... [more]
QZN05751.13.8e-2384.51cytochrome b6 [Alsophila denticulata][more]
YP_002970674.13.8e-2384.51cytochrome b6 [Alsophila spinulosa] >YP_009679435.1 cytochrome b6 [Alsophila cos... [more]
Match NameE-valueIdentityDescription
P062483.2e-2584.51Cytochrome b6 OS=Marchantia polymorpha OX=3197 GN=petB PE=3 SV=1[more]
Q3BAK74.2e-2583.10Cytochrome b6 OS=Phalaenopsis aphrodite subsp. formosana OX=308872 GN=petB PE=3 ... [more]
Q85X074.2e-2583.10Cytochrome b6 OS=Pinus koraiensis OX=88728 GN=petB PE=3 SV=1[more]
P416284.2e-2583.10Cytochrome b6 OS=Pinus thunbergii OX=3350 GN=petB PE=3 SV=2[more]
Q85A245.5e-2581.69Cytochrome b6 OS=Anthoceros angustus OX=48387 GN=petB PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A2K2CZH61.4e-2383.10CYTB_NTER domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI... [more]
A0A287WHJ41.4e-2383.10CYTB_NTER domain-containing protein OS=Hordeum vulgare subsp. vulgare OX=112509 ... [more]
A0A7L8XKV71.9e-2384.51Cytochrome b6 OS=Sphaeropteris brunoniana OX=204593 GN=petB PE=3 SV=1[more]
A0A344AIQ61.9e-2384.51Cytochrome b6 OS=Alsophila podophylla OX=204585 GN=petB PE=3 SV=1[more]
C5HY841.9e-2384.51Cytochrome b6 OS=Alsophila spinulosa OX=204586 GN=petB PE=3 SV=1[more]
Match NameE-valueIdentityDescription
ATCG00720.11.5e-2581.69photosynthetic electron transfer B [more]
AT2G07727.17.5e-0936.11Di-haem cytochrome, transmembrane;Cytochrome b/b6, C-terminal [more]
ATMG00220.17.5e-0936.11apocytochrome b [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027387Cytochrome b/b6-like domain superfamilyGENE3D1.20.810.10Cytochrome Bc1 Complex; Chain Ccoord: 1..74
e-value: 7.3E-23
score: 82.6
IPR005797Cytochrome b/b6, N-terminalPFAMPF00033Cytochrome_Bcoord: 1..71
e-value: 2.7E-26
score: 92.6
IPR005797Cytochrome b/b6, N-terminalPROSITEPS51002CYTB_NTERcoord: 1..74
score: 13.887646
IPR023530Cytochrome b6, PetBPANTHERPTHR19271:SF20SUBFAMILY NOT NAMEDcoord: 1..72
NoneNo IPR availablePANTHERPTHR19271CYTOCHROME Bcoord: 1..72
IPR016174Di-haem cytochrome, transmembraneSUPERFAMILY81342Transmembrane di-heme cytochromescoord: 1..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009397.1MS009397.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022904 respiratory electron transport chain
cellular_component GO:0016020 membrane
molecular_function GO:0009055 electron transfer activity
molecular_function GO:0016491 oxidoreductase activity