MS009360 (gene) Bitter gourd (TR) v1

Overview


Name	MS009360
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	scaffold813: 604714 .. 606058 (-)
RNA-Seq Expression	MS009360
Synteny	MS009360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGAAAGTCCACTGAAAATGACGAAATTGTCCCTACTCCGGCCAACCACGGGGTGCGGTGGCAGCGAGGGCGGCTGATCGGAAAAGGAAGCTTCGGGTCGGTTTTCATGGCGTCTTTACGGCGACCCATCTCTAAATTCAGCATTTTTCCTCCGGTGATGGCCGTCAAGTCCGCAGAAGTCTCCGATTCCGAAACCCTACAGAAGGAGAAGCAAAACTACGACAACTTGAAACAATGTAATTCGTTGATCAAATGCTTTGGAGAAGAGATCACCACCGACGAGAATGGCCAGATGATTTATAATCTGCTTCTCGAGTTCGCCGCTGCCGGAACCCTAGCCGATCAAATCAAGAACACAGGTAAATTAAAGTAGTGATCATGGGCCAGAGCTCAACCAAAAAAAAAAACATAAAATTTTCCATCTAACCCTAAAATCTTACATGTTAAGAAAAATTTTGACTGTAACCCTAAAATTTAGAACGGAATAAATTTAGGGTTTAATGAAAATTTTTATGGATTTACAGGTAAATTAAAGTAGTGATCATGGGGCGGAGCTAACAATAAAATTTTTCATCTAACCCTAAAAATCTTACATGTTTAGAATGTAATAAATTTTGGGTTTAATTTAGAATGGGAAAAAAACTACATTGGAATGTGAAATTTTGTGTGTTTTTAAGAAATTTTTTTGATGGGTATTTTCAGGTGGGAAGGGTTTGGAGGAGAAGGAAGTTTGGAGCTACACAAGATCAATAATCAAAGGGCTAATTCACATTCACAAGAACCAATACGTGCACTGCGATTTGAAGCCGGAGAATATTTTGCTGGTGCCGAGGAATTCGGCCAACGGTCGGCGCTGCGTGGCGAAGATCGGCGATTTGGGGCTGGCGAAGAGGACCAGCAAGACAAAGAAAGACCGTTACTTGAGAGGAACGGTGCCGTACATGGCGCCGGAGACATTGCTTTACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCGCTCGGCTGCGTCGTGCTGGAGATGCTCACCGGAAACCGCCCCTGGAGCGGCGGCGGCGGCGGCGACGACACGGAGGAGATTACGAGACGTTTTGGTGAGAAGTACGAGATTCCGATTATTCCGGAGGGAGCGGTGCTGTCGGCGGAGGCGAGGGCGTTTTTGAAGGTGTGTTTTGTGAGGAAGCCGGAGTATAGGTTCACGGCGGAGATGCTGATGTTGCTGCCGTTTTTGGCGGCGGCCGCGGCGGAGGACGAGGAGGAAGAAGAATCTGTGGTGGGTGCTCCAAGATTTGTGGAGAAGGCTAGGAGGTCGAAGAGGCAAAGGATTCCAATTCAAGCTGTG

mRNA sequence

ATGAAGAGAAAGTCCACTGAAAATGACGAAATTGTCCCTACTCCGGCCAACCACGGGGTGCGGTGGCAGCGAGGGCGGCTGATCGGAAAAGGAAGCTTCGGGTCGGTTTTCATGGCGTCTTTACGGCGACCCATCTCTAAATTCAGCATTTTTCCTCCGGTGATGGCCGTCAAGTCCGCAGAAGTCTCCGATTCCGAAACCCTACAGAAGGAGAAGCAAAACTACGACAACTTGAAACAATGTAATTCGTTGATCAAATGCTTTGGAGAAGAGATCACCACCGACGAGAATGGCCAGATGATTTATAATCTGCTTCTCGAGTTCGCCGCTGCCGGAACCCTAGCCGATCAAATCAAGAACACAGGTGGGAAGGGTTTGGAGGAGAAGGAAGTTTGGAGCTACACAAGATCAATAATCAAAGGGCTAATTCACATTCACAAGAACCAATACGTGCACTGCGATTTGAAGCCGGAGAATATTTTGCTGGTGCCGAGGAATTCGGCCAACGGTCGGCGCTGCGTGGCGAAGATCGGCGATTTGGGGCTGGCGAAGAGGACCAGCAAGACAAAGAAAGACCGTTACTTGAGAGGAACGGTGCCGTACATGGCGCCGGAGACATTGCTTTACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCGCTCGGCTGCGTCGTGCTGGAGATGCTCACCGGAAACCGCCCCTGGAGCGGCGGCGGCGGCGGCGACGACACGGAGGAGATTACGAGACGTTTTGGTGAGAAGTACGAGATTCCGATTATTCCGGAGGGAGCGGTGCTGTCGGCGGAGGCGAGGGCGTTTTTGAAGGTGTGTTTTGTGAGGAAGCCGGAGTATAGGTTCACGGCGGAGATGCTGATGTTGCTGCCGTTTTTGGCGGCGGCCGCGGCGGAGGACGAGGAGGAAGAAGAATCTGTGGTGGGTGCTCCAAGATTTGTGGAGAAGGCTAGGAGGTCGAAGAGGCAAAGGATTCCAATTCAAGCTGTG

Coding sequence (CDS)

ATGAAGAGAAAGTCCACTGAAAATGACGAAATTGTCCCTACTCCGGCCAACCACGGGGTGCGGTGGCAGCGAGGGCGGCTGATCGGAAAAGGAAGCTTCGGGTCGGTTTTCATGGCGTCTTTACGGCGACCCATCTCTAAATTCAGCATTTTTCCTCCGGTGATGGCCGTCAAGTCCGCAGAAGTCTCCGATTCCGAAACCCTACAGAAGGAGAAGCAAAACTACGACAACTTGAAACAATGTAATTCGTTGATCAAATGCTTTGGAGAAGAGATCACCACCGACGAGAATGGCCAGATGATTTATAATCTGCTTCTCGAGTTCGCCGCTGCCGGAACCCTAGCCGATCAAATCAAGAACACAGGTGGGAAGGGTTTGGAGGAGAAGGAAGTTTGGAGCTACACAAGATCAATAATCAAAGGGCTAATTCACATTCACAAGAACCAATACGTGCACTGCGATTTGAAGCCGGAGAATATTTTGCTGGTGCCGAGGAATTCGGCCAACGGTCGGCGCTGCGTGGCGAAGATCGGCGATTTGGGGCTGGCGAAGAGGACCAGCAAGACAAAGAAAGACCGTTACTTGAGAGGAACGGTGCCGTACATGGCGCCGGAGACATTGCTTTACAGCGTGCAAGAATCGCCGAGTGATATTTGGGCGCTCGGCTGCGTCGTGCTGGAGATGCTCACCGGAAACCGCCCCTGGAGCGGCGGCGGCGGCGGCGACGACACGGAGGAGATTACGAGACGTTTTGGTGAGAAGTACGAGATTCCGATTATTCCGGAGGGAGCGGTGCTGTCGGCGGAGGCGAGGGCGTTTTTGAAGGTGTGTTTTGTGAGGAAGCCGGAGTATAGGTTCACGGCGGAGATGCTGATGTTGCTGCCGTTTTTGGCGGCGGCCGCGGCGGAGGACGAGGAGGAAGAAGAATCTGTGGTGGGTGCTCCAAGATTTGTGGAGAAGGCTAGGAGGTCGAAGAGGCAAAGGATTCCAATTCAAGCTGTG

Protein sequence

MKRKSTENDEIVPTPANHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAEDEEEEESVVGAPRFVEKARRSKRQRIPIQAV

Homology

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BLAST of MS009360 vs. NCBI nr
Match: XP_022154776.1 (mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 325/334 (97.31%), Postives = 328/334 (98.20%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPANHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           MKRKSTENDEIVPTP NHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA
Sbjct: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60

Query: 61  EVSDSETLQKEKQNYDNLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKN 120
           EVSDSETLQKEKQNYDNLKQC+SLIKCFGEEITTDENGQMIYNLLLEFA AGTLADQIKN
Sbjct: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL 180
           TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL
Sbjct: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL 180

Query: 181 GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG 240
           GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG
Sbjct: 181 GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG 240

Query: 241 GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAA 300
           GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFL   
Sbjct: 241 GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFL--- 300

Query: 301 AAEDEEEEESVVGAPRFVEKARRSKRQRIPIQAV 335
           A ++EEEEESVVGAPRFVEKARRSKRQRIPIQAV
Sbjct: 301 AEDEEEEEESVVGAPRFVEKARRSKRQRIPIQAV 331

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BLAST of MS009360 vs. NCBI nr
Match: XP_008461430.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo])

HSP 1 Score: 406.0 bits (1042), Expect = 3.2e-109
Identity = 222/337 (65.88%), Postives = 258/337 (76.56%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPANHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           M  K     E   T  N+G++W+RGRLIGKGSFGSVF+A L+   +K+SIFPPVMAVKSA
Sbjct: 6   MLEKEAAQVEEGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPPVMAVKSA 65

Query: 61  EVSDSETLQKEKQNYDNLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKN 120
           E+S SETLQKEKQNYDNLK CNSLI+CFGEEITTD NG+MIYNLLLE A  GTLA  IKN
Sbjct: 66  EISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGTLAHHIKN 125

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPR-NSANGRRCVAKIGD 180
           TGGKGLEE  V +YT+SIIKGLIHIH++QYVHCDLKP NILL+P+ N+   R+ +AKI D
Sbjct: 126 TGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIAD 185

Query: 181 LGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGG 240
           LGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR W+   
Sbjct: 186 LGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGNRAWAATD 245

Query: 241 GGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAA 300
                +E+T  F     +P IPEG  LSAEA  FLK C VRKPE+RFTAEML+ +PF+  
Sbjct: 246 KVGILQEMTENF---LGMPKIPEG--LSAEATGFLKNCLVRKPEFRFTAEMLINVPFV-- 305

Query: 301 AAAEDEEEEESVVGAPRFVEK-ARRSKRQR-IPIQAV 335
           AAAED+E++ + V AP FV K  R+ KRQR IPI+AV
Sbjct: 306 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

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BLAST of MS009360 vs. NCBI nr
Match: XP_004136102.2 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus])

HSP 1 Score: 403.3 bits (1035), Expect = 2.1e-108
Identity = 222/342 (64.91%), Postives = 258/342 (75.44%), Query Frame = 0


Query: 1   MKRKSTENDEIV-----PTPANHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVM 60
           MKRKS    E        +  N+G++W++GRLIGKGSFGSVF+ASL+   +K+SIFPPVM
Sbjct: 1   MKRKSMLEKEAAQVEDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVM 60

Query: 61  AVKSAEVSDSETLQKEKQNYDNLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLA 120
           AVKSAE+S SETLQKEKQNYDNLK CNSLI+CFGEEITTD NG MIYNLLLE A  GTLA
Sbjct: 61  AVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLA 120

Query: 121 DQIKNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPR-NSANGRRCV 180
             IKNTGGKGLEE  V +YT+SIIKGLIH+H++QYVHCDLKP NILL+P+ N+   R+ +
Sbjct: 121 HHIKNTGGKGLEENVVRNYTKSIIKGLIHMHRSQYVHCDLKPANILLLPKNNTTKDRQFI 180

Query: 181 AKIGDLGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRP 240
           AKI DLGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR 
Sbjct: 181 AKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRA 240

Query: 241 WSGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLL 300
           W+        +E+T  F     +P IPEG  LS EA  FLK C VRKPE+RFTAEMLM +
Sbjct: 241 WAATDKVGIVKEMTENF---IGMPKIPEG--LSPEATGFLKNCLVRKPEFRFTAEMLMNV 300

Query: 301 PFLAAAAAEDEEEEESVVGAPRFVEK-ARRSKRQR-IPIQAV 335
           PF+  AA ED E++ + V AP FV K  R+ KRQR IPI+AV
Sbjct: 301 PFV--AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]

BLAST of MS009360 vs. NCBI nr
Match: KAG7014197.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 401.4 bits (1030), Expect = 7.8e-108
Identity = 217/327 (66.36%), Postives = 248/327 (75.84%), Query Frame = 0


Query: 17  NHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYD 76
           N GV+W+RGRLIGKGSFGSVFMASL+R  ++FSIFPPVMAVKSAEVS S++LQKEKQ YD
Sbjct: 26  NDGVQWERGRLIGKGSFGSVFMASLKRSTAEFSIFPPVMAVKSAEVSVSKSLQKEKQIYD 85

Query: 77  NLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTR 136
           NLK CNSLI+CFGEEITTD NG MIYNLLLE A  G+LA +I NTGGKGL E EV  YT+
Sbjct: 86  NLKGCNSLIQCFGEEITTDRNGPMIYNLLLEIALGGSLAHRINNTGGKGLAENEVGRYTK 145

Query: 137 SIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLR 196
           +IIKGLIHIH+  YVHCDLKP NILL+P N+A G R VAKI DLGLA+RTS+T+      
Sbjct: 146 TIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGGRLVAKICDLGLARRTSETRHGNCFG 205

Query: 197 GTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYE 256
           GT+ YMAPETL++ VQESPSDIWALGC+VLEMLTGNRPW+      D   I +   E   
Sbjct: 206 GTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNRPWA----ATDEAGIVKSITENDG 265

Query: 257 IPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAA------AAAEDEEEEES 316
           +P IPEG  +S EA  FLK CFVR PEYRFTAEMLM +PF+AA         E+EEEEE 
Sbjct: 266 MPEIPEG--VSKEASGFLKNCFVRNPEYRFTAEMLMTVPFVAAEEEEEEEEEEEEEEEED 325

Query: 317 VVGAPRFVEKARRS--KRQRI-PIQAV 335
            + A  FV K  RS  KRQRI P +AV
Sbjct: 326 FMRALTFVRKRSRSCRKRQRIVPTEAV 346

[Close]

BLAST of MS009360 vs. NCBI nr
Match: KAE8646420.1 (hypothetical protein Csa_015819 [Cucumis sativus])

HSP 1 Score: 401.0 bits (1029), Expect = 1.0e-107
Identity = 216/321 (67.29%), Postives = 251/321 (78.19%), Query Frame = 0


Query: 17  NHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYD 76
           N+G++W++GRLIGKGSFGSVF+ASL+   +K+SIFPPVMAVKSAE+S SETLQKEKQNYD
Sbjct: 17  NNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVMAVKSAEISVSETLQKEKQNYD 76

Query: 77  NLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTR 136
           NLK CNSLI+CFGEEITTD NG MIYNLLLE A  GTLA  IKNTGGKGLEE  V +YT+
Sbjct: 77  NLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLAHHIKNTGGKGLEENVVRNYTK 136

Query: 137 SIIKGLIHIHKNQYVHCDLKPENILLVPR-NSANGRRCVAKIGDLGLAKRTSKTKKDRYL 196
           SIIKGLIH+H++QYVHCDLKP NILL+P+ N+   R+ +AKI DLGLA+RTSKTK    L
Sbjct: 137 SIIKGLIHMHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIADLGLARRTSKTKASYCL 196

Query: 197 RGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKY 256
            GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR W+        +E+T  F    
Sbjct: 197 GGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRAWAATDKVGIVKEMTENF---I 256

Query: 257 EIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAEDEEEEESVVGAP 316
            +P IPEG  LS EA  FLK C VRKPE+RFTAEMLM +PF+  AA ED E++ + V AP
Sbjct: 257 GMPKIPEG--LSPEATGFLKNCLVRKPEFRFTAEMLMNVPFV--AAGEDHEQDSTTVKAP 316

Query: 317 RFVEK-ARRSKRQR-IPIQAV 335
            FV K  R+ KRQR IPI+AV
Sbjct: 317 TFVTKWPRQFKRQRTIPIKAV 330

[Close]

BLAST of MS009360 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 5.5e-48
Identity = 117/292 (40.07%), Postives = 164/292 (56.16%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RG  IG G+F +V  A+  R       FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSR---NSGDFPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 80  QCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
            C  +I+C+GE+ +T ENG+ ++NLLLE+A+ G+LA  +K  GG+GL E  V  +T S++
Sbjct: 61  DCPEIIRCYGED-STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRT----SKTKKDRYL 199
           +GL HIH   + HCD+K  NILL    S        KI D GLA R     +  +K   +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 200 RGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKY 259
           RGT  YMAPE +  +   S +D+WALGC V+EM +G   WS    G     +  R G   
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWS-VKEGSHFMSLLIRIGVGD 240

Query: 260 EIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAEDEEE 308
           E+P IPE  +LS E + FL  CFV+ P  R+TAEML+   F+     +D  E
Sbjct: 241 ELPKIPE--MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRE 278

[Close]

BLAST of MS009360 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.0e-41
Identity = 103/278 (37.05%), Postives = 151/278 (54.32%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RGR++G+GS  +V+ A+             ++AVKS+EV  SE LQ+E +   +L 
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNS-------DEILAVKSSEVHRSEFLQREAKILSSLS 60

Query: 80  QCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
               +I   G E   + NG ++YNLL+E+A  GTL D     GG+ ++E  V  YTR I+
Sbjct: 61  S-PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGR-VDETRVVKYTRDIL 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTV 199
           KGL +IH    VHCD+K  N+++  +         AKI D G AKR     +   + GT 
Sbjct: 121 KGLEYIHSKGIVHCDVKGSNVVISEKGE-------AKIADFGCAKRVDPVFESPVM-GTP 180

Query: 200 PYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYEIPI 259
            +MAPE      Q   SDIWA+GC ++EM+TG+ PW+     +D   +  R G   E P 
Sbjct: 181 AFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPE 240

Query: 260 IPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFL 298
           +P   +L+ EA+ FL+ C  R+   R+TA  L+  PFL
Sbjct: 241 LP--CLLAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

[Close]

BLAST of MS009360 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.5e-32
Identity = 107/299 (35.79%), Postives = 153/299 (51.17%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RG+ +G+GS  +V  A+              +AVKSAE   SE LQ+E +   +L 
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHES-------GETLAVKSAEFHRSEFLQREAKILSSLN 60

Query: 80  QCNSLIKCFGEEITTD---ENGQ-MIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYT 139
               +I   G EIT +    NG+   Y+LL+E+A  GTL D     GG  ++E  V  YT
Sbjct: 61  S-PYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGG-FIDEARVVKYT 120

Query: 140 RSIIKGLIHIHKNQYV-HCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRY 199
           R I+ GL +IH ++ + HCD+K  N+L+       G    AKI D G AK   + +    
Sbjct: 121 RQILLGLEYIHNSKGIAHCDIKGSNVLV-------GENGEAKIADFGCAKWV-EPEITEP 180

Query: 200 LRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEK 259
           +RGT  +MAPE      Q   SDIWA+GC V+EM+TG++PW G    D    +  R G  
Sbjct: 181 VRGTPAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPV-SVLYRVGYL 240

Query: 260 YEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAEDEEEEESVVG 314
            E+P +P    L+ +A+ FL  C  ++   R+TA  L+  PFL        +E E V G
Sbjct: 241 GELPELP--CSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLV------NKEPELVTG 273

[Close]

BLAST of MS009360 vs. ExPASy Swiss-Prot
Match: P25390 (Serine/threonine-protein kinase SSK22 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SSK22 PE=1 SV=2)

HSP 1 Score: 130.6 bits (327), Expect = 3.4e-29
Identity = 100/309 (32.36%), Postives = 154/309 (49.84%), Query Frame = 0


Query: 16   ANHGVRWQRGRLIGKGSFGSVFMA-SLRRPISKFSIFPPVMAVKSAEVSDSETLQK---- 75
            +N  +RWQ+   IG G+FG V+ A +L            ++AVK  ++ D+ T++K    
Sbjct: 1028 SNVSMRWQKRSFIGGGTFGQVYSAINLEN--------GEILAVKEIKIHDTTTMKKIFPL 1087

Query: 76   EKQNYDNLKQCN--SLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEE 135
             K+    L+  N  ++++ +G E+  D+      N+ +E+   G+LA  + +  G+  +E
Sbjct: 1088 IKEEMTVLEMLNHPNIVQYYGVEVHRDK-----VNIFMEYCEGGSLASLLDH--GRIEDE 1147

Query: 136  KEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAK---- 195
                 YT  +++GL ++H++  VH D+KPENILL      NG   + K  D G A+    
Sbjct: 1148 MVTQVYTFELLEGLAYLHQSGVVHRDIKPENILL----DFNG---IIKYVDFGTARTVVG 1207

Query: 196  -RTS------------KTKKDRYLRGTVPYMAPETLLYSVQE---SPSDIWALGCVVLEM 255
             RT             +TK    + GT  YMAPET+  S  +      D+WALGCVVLEM
Sbjct: 1208 SRTRTVRNAAVQDFGVETKSLNEMMGTPMYMAPETISGSAVKGKLGADDVWALGCVVLEM 1267

Query: 256  LTGNRPWSGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTA 298
             TG RPWS      D E           IP +P    ++A  RAFL+ C V+ P  R TA
Sbjct: 1268 ATGRRPWS----NLDNEWAIMYHVAAGRIPQLPNRDEMTAAGRAFLERCLVQDPTMRATA 1310

[Close]

BLAST of MS009360 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 124.8 bits (312), Expect = 1.9e-27
Identity = 85/302 (28.15%), Postives = 149/302 (49.34%), Query Frame = 0


Query: 7   ENDEIVPTPANHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSE 66
           E++E++   +NH ++WQ+G+++G+G +GSV++  L +   +      + AVK  E+ D  
Sbjct: 158 EHEELI---SNHNIKWQKGQILGRGGYGSVYL-GLNKDTGE------LFAVKQLEIVDIN 217

Query: 67  ----------TLQKEKQNYDNLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLAD 126
                     +  KE +   +L+  N +++  G  +      Q   ++ LE+   G+++ 
Sbjct: 218 SDPKLKNMILSFSKEIEVMRSLRHDN-IVRYLGTSL-----DQSFLSVFLEYIPGGSISS 277

Query: 127 QIKNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAK 186
            +   G     E  +  YT+ I++GL  +H N  +H D+K  NIL+  +        + K
Sbjct: 278 LLGKFG--AFSENVIKVYTKQILQGLSFLHANSIIHRDIKGANILIDTKG-------IVK 337

Query: 187 IGDLGLAKRTSK-TKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPW 246
           + D G +K  S    + + ++GT  +MAPE +  +     SDIW+LGCV++EM T   PW
Sbjct: 338 LSDFGCSKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLGCVIVEMATAQPPW 397

Query: 247 SGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLP 298
           S      +   +         IP IP  + +S EA  FL +CF R P+ R  A  L+  P
Sbjct: 398 S---NITELAAVMYHIASSNSIPNIP--SHMSQEAFDFLNLCFKRDPKERPDANQLLKHP 429

[Close]

BLAST of MS009360 vs. ExPASy TrEMBL
Match: A0A6J1DPQ9 (mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX=3673 GN=LOC111021943 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.1e-183
Identity = 325/334 (97.31%), Postives = 328/334 (98.20%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPANHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           MKRKSTENDEIVPTP NHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA
Sbjct: 1   MKRKSTENDEIVPTPVNHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60

Query: 61  EVSDSETLQKEKQNYDNLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKN 120
           EVSDSETLQKEKQNYDNLKQC+SLIKCFGEEITTDENGQMIYNLLLEFA AGTLADQIKN
Sbjct: 61  EVSDSETLQKEKQNYDNLKQCDSLIKCFGEEITTDENGQMIYNLLLEFATAGTLADQIKN 120

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL 180
           TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL
Sbjct: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDL 180

Query: 181 GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG 240
           GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG
Sbjct: 181 GLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGG 240

Query: 241 GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAA 300
           GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFL   
Sbjct: 241 GDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFL--- 300

Query: 301 AAEDEEEEESVVGAPRFVEKARRSKRQRIPIQAV 335
           A ++EEEEESVVGAPRFVEKARRSKRQRIPIQAV
Sbjct: 301 AEDEEEEEESVVGAPRFVEKARRSKRQRIPIQAV 331

[Close]

BLAST of MS009360 vs. ExPASy TrEMBL
Match: A0A1S3CFZ6 (mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN=LOC103500025 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.5e-109
Identity = 222/337 (65.88%), Postives = 258/337 (76.56%), Query Frame = 0


Query: 1   MKRKSTENDEIVPTPANHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSA 60
           M  K     E   T  N+G++W+RGRLIGKGSFGSVF+A L+   +K+SIFPPVMAVKSA
Sbjct: 6   MLEKEAAQVEEGSTEFNNGIQWKRGRLIGKGSFGSVFLACLKPHFTKYSIFPPVMAVKSA 65

Query: 61  EVSDSETLQKEKQNYDNLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKN 120
           E+S SETLQKEKQNYDNLK CNSLI+CFGEEITTD NG+MIYNLLLE A  GTLA  IKN
Sbjct: 66  EISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGRMIYNLLLEVATGGTLAHHIKN 125

Query: 121 TGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPR-NSANGRRCVAKIGD 180
           TGGKGLEE  V +YT+SIIKGLIHIH++QYVHCDLKP NILL+P+ N+   R+ +AKI D
Sbjct: 126 TGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIAD 185

Query: 181 LGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGG 240
           LGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR W+   
Sbjct: 186 LGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGNRAWAATD 245

Query: 241 GGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAA 300
                +E+T  F     +P IPEG  LSAEA  FLK C VRKPE+RFTAEML+ +PF+  
Sbjct: 246 KVGILQEMTENF---LGMPKIPEG--LSAEATGFLKNCLVRKPEFRFTAEMLINVPFV-- 305

Query: 301 AAAEDEEEEESVVGAPRFVEK-ARRSKRQR-IPIQAV 335
           AAAED+E++ + V AP FV K  R+ KRQR IPI+AV
Sbjct: 306 AAAEDQEQDFNTVKAPTFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of MS009360 vs. ExPASy TrEMBL
Match: A0A0A0KBM3 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430800 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 4.5e-109
Identity = 223/342 (65.20%), Postives = 258/342 (75.44%), Query Frame = 0


Query: 1   MKRKSTENDEIV-----PTPANHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVM 60
           MKRKS    E        +  N+G++W++GRLIGKGSFGSVF+ASL+   +K+SIFPPVM
Sbjct: 1   MKRKSMLEKEAAQVEDRSSEFNNGIQWKQGRLIGKGSFGSVFLASLKPHFTKYSIFPPVM 60

Query: 61  AVKSAEVSDSETLQKEKQNYDNLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLA 120
           AVKSAE+S SETLQKEKQNYDNLK CNSLI+CFGEEITTD NG MIYNLLLE A  GTLA
Sbjct: 61  AVKSAEISVSETLQKEKQNYDNLKGCNSLIQCFGEEITTDHNGHMIYNLLLEVATGGTLA 120

Query: 121 DQIKNTGGKGLEEKEVWSYTRSIIKGLIHIHKNQYVHCDLKPENILLVPR-NSANGRRCV 180
             IKNTGGKGLEE  V +YT+SIIKGLIHIH++QYVHCDLKP NILL+P+ N+   R+ +
Sbjct: 121 HHIKNTGGKGLEENVVRNYTKSIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFI 180

Query: 181 AKIGDLGLAKRTSKTKKDRYLRGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRP 240
           AKI DLGLA+RTSKTK    L GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR 
Sbjct: 181 AKIADLGLARRTSKTKASYCLGGTFSYMAPETLIDGVQESASDIWALGCVVLEMLTGNRA 240

Query: 241 WSGGGGGDDTEEITRRFGEKYEIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLL 300
           W+        +E+T  F     +P IPEG  LS EA  FLK C VRKPE+RFTAEMLM +
Sbjct: 241 WAATDKVGIVKEMTENF---IGMPKIPEG--LSPEATGFLKNCLVRKPEFRFTAEMLMNV 300

Query: 301 PFLAAAAAEDEEEEESVVGAPRFVEK-ARRSKRQR-IPIQAV 335
           PF+  AA ED E++ + V AP FV K  R+ KRQR IPI+AV
Sbjct: 301 PFV--AAGEDHEQDSTTVKAPTFVTKWPRQFKRQRTIPIKAV 335

[Close]

BLAST of MS009360 vs. ExPASy TrEMBL
Match: A0A5D3CH22 (Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001440 PE=3 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 6.5e-108
Identity = 217/321 (67.60%), Postives = 252/321 (78.50%), Query Frame = 0


Query: 17  NHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYD 76
           N+G++W+RGRLIGKGSFGSVF+A L+    K+SIFPPVMAVKSAE+S SETLQKEKQNYD
Sbjct: 22  NNGIQWKRGRLIGKGSFGSVFLACLKPHFIKYSIFPPVMAVKSAEISVSETLQKEKQNYD 81

Query: 77  NLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTR 136
           NLK CNSLI+CFGEEITTD NG+M YNLLLE A  GTLA  IKNTGGKGLEE  V +YT+
Sbjct: 82  NLKGCNSLIQCFGEEITTDHNGRMTYNLLLEVATGGTLAHHIKNTGGKGLEENVVRNYTK 141

Query: 137 SIIKGLIHIHKNQYVHCDLKPENILLVPR-NSANGRRCVAKIGDLGLAKRTSKTKKDRYL 196
           SIIKGLIHIH++QYVHCDLKP NILL+P+ N+   R+ +AKI DLGLA+RTSKTK    L
Sbjct: 142 SIIKGLIHIHRSQYVHCDLKPANILLLPKNNTTKDRQFIAKIADLGLARRTSKTKGSYCL 201

Query: 197 RGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKY 256
            GT  YMAPETL+  VQES SDIWALGCVVLEMLTGNR W+        +E+T  F    
Sbjct: 202 GGTFSYMAPETLIDGVQESTSDIWALGCVVLEMLTGNRAWAATDKVGIVQEMTENF---L 261

Query: 257 EIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAEDEEEEESVVGAP 316
            +P IPEG  LSAEA  FLK C VRKPE+RFTAEML+ +PF+  AAAED+E++ + V AP
Sbjct: 262 GMPKIPEG--LSAEATGFLKNCLVRKPEFRFTAEMLINVPFV--AAAEDQEQDFNTVKAP 321

Query: 317 RFVEK-ARRSKRQR-IPIQAV 335
            FV K  R+ KRQR IPI+AV
Sbjct: 322 TFVTKWPRQFKRQRIIPIKAV 335

[Close]

BLAST of MS009360 vs. ExPASy TrEMBL
Match: A0A6J1GPL4 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111456326 PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 9.3e-107
Identity = 216/323 (66.87%), Postives = 248/323 (76.78%), Query Frame = 0


Query: 17  NHGVRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYD 76
           N GV+W+RGRLIGKGSFGSVFMASL+R  S+FSIFPPVMAVKSAEVS S++LQKEKQ YD
Sbjct: 22  NDGVQWERGRLIGKGSFGSVFMASLKR--SEFSIFPPVMAVKSAEVSVSKSLQKEKQIYD 81

Query: 77  NLKQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTR 136
           NLK CNSLI+CFGEEITTD NG+MIYNL LE A  G+LA +I N GGKGL E EV  YT+
Sbjct: 82  NLKGCNSLIQCFGEEITTDRNGRMIYNLRLEIALGGSLAHRINNIGGKGLAENEVGRYTK 141

Query: 137 SIIKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLR 196
           +IIKGLIHIH+  YVHCDLKP NILL+P N+A G R VAKI DLGLA+RTS+T+      
Sbjct: 142 TIIKGLIHIHRRGYVHCDLKPANILLLPTNTAKGGRLVAKICDLGLARRTSETRHGNCFG 201

Query: 197 GTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYE 256
           GT+ YMAPETL++ VQESPSDIWALGC+VLEMLTGNRPW+      D   I +   E   
Sbjct: 202 GTLSYMAPETLIHRVQESPSDIWALGCLVLEMLTGNRPWA----ATDEAGIVKSITENDG 261

Query: 257 IPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAA--AAEDEEEEESVVGA 316
           +P IPEG  +S +A  FLK CFVR PEYRFTAEMLM +PF+AAA    E+EEEEE  + A
Sbjct: 262 MPEIPEG--VSKDASGFLKNCFVRNPEYRFTAEMLMTVPFVAAAEEEEEEEEEEEDFMRA 321

Query: 317 PRFVEKARRS--KRQRI-PIQAV 335
             FV K  RS  KRQRI P +AV
Sbjct: 322 LTFVRKRSRSCRKRQRIVPTEAV 336

[Close]

BLAST of MS009360 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 208.0 bits (528), Expect = 1.2e-53
Identity = 131/298 (43.96%), Postives = 178/298 (59.73%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W R   IG GSF +V +A+     SK   FP +MAVKS+ V  S  L+ E+   D+L 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSK--AFPSLMAVKSSGVVCSAALRNERDVLDDLG 60

Query: 80  QCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
            C+ +++CFGE  T  ENG+ IYNL LE+A+ G+LAD+IK++ G+ L E EV  +TRSI+
Sbjct: 61  DCSEIVRCFGEGRTV-ENGEEIYNLFLEYASGGSLADRIKSS-GEALPEFEVRRFTRSIV 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTV 199
           KGL HIH N + HCD+K EN+L+             KI D GLAKR S  +    +RGT 
Sbjct: 121 KGLCHIHGNGFTHCDIKLENVLVFGDGD-------VKISDFGLAKRRS-GEVCVEIRGTP 180

Query: 200 PYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWS-GGGGGDDTEEITRRFGEKYEIP 259
            YMAPE++ +   ESP+DIWALGC V+EM +G   W    G  ++   +  R G   E+P
Sbjct: 181 LYMAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVP 240

Query: 260 IIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAEDEEEEESVVGAPR 317
            IP    LS E + F+  CFV+    R+TAEML+  PFL A   E  EE+E+   +PR
Sbjct: 241 RIP--VELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFL-AVDDESGEEDEACSVSPR 283

[Close]

BLAST of MS009360 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 203.0 bits (515), Expect = 3.8e-52
Identity = 125/299 (41.81%), Postives = 173/299 (57.86%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNL- 79
           + W RG  IG G+F +V +A+  R  +    FPP+MAVKSA+   + +L  EK   DNL 
Sbjct: 1   MEWIRGETIGYGTFSTVSLAT--RSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLG 60

Query: 80  KQCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTRSI 139
             CN +++CFGE+ T  ENG+ ++NL LE+A+ G+L   +K   G+G+ E  V  +T S+
Sbjct: 61  DDCNEIVRCFGEDRTV-ENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 140 IKGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRY---L 199
           ++GL HIH N + HCDLK  NILL    +        KI D GLAKR        Y   +
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDGA-------VKIADFGLAKRIGDLTALNYGVQI 180

Query: 200 RGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKY 259
           RGT  YMAPE++  +   S  D+WALGCVV+EM +G   WS    G +   +  R G   
Sbjct: 181 RGTPLYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWS-LKEGSNFMSLLLRIGVGD 240

Query: 260 EIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAED--EEEEESVV 313
           E+P+IPE   LS + R FL  CFV+ P+ R+TAEML+  PF+      D   +EE+ VV
Sbjct: 241 EVPMIPE--ELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVV 286

[Close]

BLAST of MS009360 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 193.0 bits (489), Expect = 3.9e-49
Identity = 117/292 (40.07%), Postives = 164/292 (56.16%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RG  IG G+F +V  A+  R       FP ++AVKS +   + +L  EK   D+L 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSR---NSGDFPALIAVKSTDAYGAASLSNEKSVLDSLG 60

Query: 80  QCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
            C  +I+C+GE+ +T ENG+ ++NLLLE+A+ G+LA  +K  GG+GL E  V  +T S++
Sbjct: 61  DCPEIIRCYGED-STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVL 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRT----SKTKKDRYL 199
           +GL HIH   + HCD+K  NILL    S        KI D GLA R     +  +K   +
Sbjct: 121 RGLRHIHAKGFAHCDIKLANILLFNDGS-------VKIADFGLAMRVDGDLTALRKSVEI 180

Query: 200 RGTVPYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKY 259
           RGT  YMAPE +  +   S +D+WALGC V+EM +G   WS    G     +  R G   
Sbjct: 181 RGTPLYMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWS-VKEGSHFMSLLIRIGVGD 240

Query: 260 EIPIIPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAEDEEE 308
           E+P IPE  +LS E + FL  CFV+ P  R+TAEML+   F+     +D  E
Sbjct: 241 ELPKIPE--MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRE 278

[Close]

BLAST of MS009360 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 178.3 bits (451), Expect = 1.0e-44
Identity = 112/291 (38.49%), Postives = 163/291 (56.01%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RG +IG+GS  +V +A         S    + AVKSA++S S  LQKE+     L 
Sbjct: 3   INWTRGPIIGRGSTATVSIA--------ISSSGELFAVKSADLSSSSLLQKEQSILSTLS 62

Query: 80  QCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
             + ++K  G  +T + NG ++YN+L+E+ + G L D IKN+GGK L E E+ SYTR I+
Sbjct: 63  SPH-MVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGK-LPEPEIRSYTRQIL 122

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTV 199
            GL+++H+   VHCDLK  N+L+      NG   V KI D+G AK   K++      GT 
Sbjct: 123 NGLVYLHERGIVHCDLKSHNVLV----EENG---VLKIADMGCAKSVDKSE----FSGTP 182

Query: 200 PYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYEIPI 259
            +MAPE      Q  P+D+WALGC ++EM+TG+ PW      +D      + G   E P 
Sbjct: 183 AFMAPEVARGEEQRFPADVWALGCTMIEMMTGSSPWP---ELNDVVAAMYKIGFSGESPA 242

Query: 260 IPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFLAAAAAEDEEEEES 311
           IP  A +S +A+ FLK C     + R+T E L+  PFL     +D+EE ++
Sbjct: 243 IP--AWISDKAKDFLKNCLKEDQKQRWTVEELLKHPFL-----DDDEESQT 261

[Close]

BLAST of MS009360 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 172.2 bits (435), Expect = 7.2e-43
Identity = 103/278 (37.05%), Postives = 151/278 (54.32%), Query Frame = 0


Query: 20  VRWQRGRLIGKGSFGSVFMASLRRPISKFSIFPPVMAVKSAEVSDSETLQKEKQNYDNLK 79
           + W RGR++G+GS  +V+ A+             ++AVKS+EV  SE LQ+E +   +L 
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNS-------DEILAVKSSEVHRSEFLQREAKILSSLS 60

Query: 80  QCNSLIKCFGEEITTDENGQMIYNLLLEFAAAGTLADQIKNTGGKGLEEKEVWSYTRSII 139
               +I   G E   + NG ++YNLL+E+A  GTL D     GG+ ++E  V  YTR I+
Sbjct: 61  S-PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGR-VDETRVVKYTRDIL 120

Query: 140 KGLIHIHKNQYVHCDLKPENILLVPRNSANGRRCVAKIGDLGLAKRTSKTKKDRYLRGTV 199
           KGL +IH    VHCD+K  N+++  +         AKI D G AKR     +   + GT 
Sbjct: 121 KGLEYIHSKGIVHCDVKGSNVVISEKGE-------AKIADFGCAKRVDPVFESPVM-GTP 180

Query: 200 PYMAPETLLYSVQESPSDIWALGCVVLEMLTGNRPWSGGGGGDDTEEITRRFGEKYEIPI 259
            +MAPE      Q   SDIWA+GC ++EM+TG+ PW+     +D   +  R G   E P 
Sbjct: 181 AFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPE 240

Query: 260 IPEGAVLSAEARAFLKVCFVRKPEYRFTAEMLMLLPFL 298
           +P   +L+ EA+ FL+ C  R+   R+TA  L+  PFL
Sbjct: 241 LP--CLLAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

The following BLAST results are available for this feature:

BLAST of MS009360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154776.1	1.3e-182	97.31	mitogen-activated protein kinase kinase kinase 17-like [Momordica charantia]	[more]
XP_008461430.1	3.2e-109	65.88	PREDICTED: mitogen-activated protein kinase kinase kinase 2-like [Cucumis melo]	[more]
XP_004136102.2	2.1e-108	64.91	mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus]	[more]
KAG7014197.1	7.8e-108	66.36	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]
KAE8646420.1	1.0e-107	67.29	hypothetical protein Csa_015819 [Cucumis sativus]	[more]

BLAST of MS009360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	5.5e-48	40.07	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	1.0e-41	37.05	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9ZVP5	1.5e-32	35.79	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]
P25390	3.4e-29	32.36	Serine/threonine-protein kinase SSK22 OS=Saccharomyces cerevisiae (strain ATCC 2...	[more]
Q54R82	1.9e-27	28.15	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]

BLAST of MS009360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DPQ9	6.1e-183	97.31	mitogen-activated protein kinase kinase kinase 17-like OS=Momordica charantia OX...	[more]
A0A1S3CFZ6	1.5e-109	65.88	mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo OX=3656 GN...	[more]
A0A0A0KBM3	4.5e-109	65.20	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G430...	[more]
A0A5D3CH22	6.5e-108	67.60	Mitogen-activated protein kinase kinase kinase 2-like OS=Cucumis melo var. makuw...	[more]
A0A6J1GPL4	9.3e-107	66.87	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]

BLAST of MS009360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	1.2e-53	43.96	mitogen-activated protein kinase kinase kinase 21	[more]
AT5G67080.1	3.8e-52	41.81	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G50310.1	3.9e-49	40.07	mitogen-activated protein kinase kinase kinase 20	[more]
AT4G26890.1	1.0e-44	38.49	mitogen-activated protein kinase kinase kinase 16	[more]
AT2G32510.1	7.2e-43	37.05	mitogen-activated protein kinase kinase kinase 17	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 22..297 e-value: 5.2E-54 score: 195.4
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 23..296 e-value: 4.2E-45 score: 154.2
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 22..297 score: 38.336369
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 111..329 e-value: 2.4E-46 score: 159.8
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 19..110 e-value: 5.3E-6 score: 28.1
None	No IPR available	PIRSR	PIRSR037993-2	PIRSR037993-2	coord: 22..305 e-value: 2.4E-9 score: 34.4
None	No IPR available	PIRSR	PIRSR000550-2	PIRSR000550-2	coord: 27..288 e-value: 2.7E-22 score: 76.8
None	No IPR available	PANTHER	PTHR48011:SF56	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKE	coord: 19..311
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 19..311
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 28..58
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 150..162
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 21..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009360.1	MS009360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity