MS009358 (gene) Bitter gourd (TR) v1

BLAST of MS009358 vs. NCBI nr
Match: XP_022154094.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Momordica charantia] >XP_022154101.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Momordica charantia] >XP_022154111.1 ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 825.9 bits (2132), Expect = 1.7e-235
Identity = 420/436 (96.33%), Postives = 422/436 (96.79%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAISISGLSLPSLFQYKNSTIAR+ RIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ
Sbjct: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR
Sbjct: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120

Query: 121 SFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180
           SFEYLQGDYYIAPVFM  +           VCHIVKNFIAHLLNIKVPLILGIWGGKGQG
Sbjct: 121 SFEYLQGDYYIAPVFMDKV-----------VCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180

Query: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240
           KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND
Sbjct: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240

Query: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300
           IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST
Sbjct: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300

Query: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360
           IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR
Sbjct: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360

Query: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420
           SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL
Sbjct: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420

Query: 421 IMETKLSKEYMKNMDD 437
           IMETKLSKEYMKNMDD
Sbjct: 421 IMETKLSKEYMKNMDD 425

BLAST of MS009358 vs. NCBI nr
Match: XP_022960536.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 762.3 bits (1967), Expect = 2.3e-216
Identity = 383/436 (87.84%), Postives = 405/436 (92.89%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+ISG+SLPSLF YKNS IAR +RIRSF+IRS KDV GE  GGG GEPS++ KRLSE+
Sbjct: 1   MAIAISGVSLPSLFHYKNSIIARKSRIRSFSIRSCKDVSGEGDGGG-GEPSQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLG+EADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180
           SFE+LQGDYYIAPVFM  +           VCHIVKNFI HLLNIKVPLILGIWGGKGQG
Sbjct: 121 SFEHLQGDYYIAPVFMDKV-----------VCHIVKNFIVHLLNIKVPLILGIWGGKGQG 180

Query: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240
           KSFQTELIFQ MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMIND
Sbjct: 181 KSFQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMIND 240

Query: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300
           IDAGIGRF NTQVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFST
Sbjct: 241 IDAGIGRFENTQVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFST 300

Query: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360
           IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALR
Sbjct: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALR 360

Query: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420
           SRTYD ++SKWVDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSL+KEQQ 
Sbjct: 361 SRTYDTSISKWVDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLIKEQQF 420

Query: 421 IMETKLSKEYMKNMDD 437
           +METKLSKEYMKNMDD
Sbjct: 421 VMETKLSKEYMKNMDD 424

BLAST of MS009358 vs. NCBI nr
Match: XP_023515166.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 759.6 bits (1960), Expect = 1.5e-215
Identity = 383/436 (87.84%), Postives = 404/436 (92.66%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+ISG+SLPSLF YKNS IAR +RIRSF+IRS KDV GE  G G GEPS++ KRLSE+
Sbjct: 1   MAIAISGVSLPSLFHYKNSIIARKSRIRSFSIRSCKDVSGE--GDGGGEPSQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLG+EADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180
           SFE+LQGDYYIAPVFM  +           VCHIVKNFI HLLNIKVPLILGIWGGKGQG
Sbjct: 121 SFEHLQGDYYIAPVFMDKV-----------VCHIVKNFIVHLLNIKVPLILGIWGGKGQG 180

Query: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240
           KSFQTELIFQ MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMIND
Sbjct: 181 KSFQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMIND 240

Query: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300
           IDAGIGRF NTQVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFST
Sbjct: 241 IDAGIGRFENTQVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFST 300

Query: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360
           IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALR
Sbjct: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALR 360

Query: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420
           SRTYD ++SKWVDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSLVKEQQ 
Sbjct: 361 SRTYDTSISKWVDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLVKEQQF 420

Query: 421 IMETKLSKEYMKNMDD 437
           +METKLSKEYMKNMDD
Sbjct: 421 VMETKLSKEYMKNMDD 423

BLAST of MS009358 vs. NCBI nr
Match: XP_023004209.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 759.6 bits (1960), Expect = 1.5e-215
Identity = 383/436 (87.84%), Postives = 403/436 (92.43%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+IS +SLPSLFQYKN  IAR +RIRSF+IRS KDV GE  G G GEP ++ KRLSE+
Sbjct: 1   MAIAISAVSLPSLFQYKNLIIARKSRIRSFSIRSCKDVSGE--GDGGGEPLQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180
           SFE+LQGDYYIAPVFM  +           VCHIVKNFI HLLNIKVPLILGIWGGKGQG
Sbjct: 121 SFEHLQGDYYIAPVFMDKV-----------VCHIVKNFIVHLLNIKVPLILGIWGGKGQG 180

Query: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240
           KSFQTELIFQ MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMIND
Sbjct: 181 KSFQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMIND 240

Query: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300
           IDAGIGRFGNTQVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFST
Sbjct: 241 IDAGIGRFGNTQVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFST 300

Query: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360
           IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALR
Sbjct: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALR 360

Query: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420
           SRTYD ++SKWVDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSLVKEQQ 
Sbjct: 361 SRTYDTSISKWVDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLVKEQQF 420

Query: 421 IMETKLSKEYMKNMDD 437
           +METKLSKEYMKNMDD
Sbjct: 421 VMETKLSKEYMKNMDD 423

BLAST of MS009358 vs. NCBI nr
Match: XP_038900248.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 754.6 bits (1947), Expect = 4.7e-214
Identity = 381/438 (86.99%), Postives = 404/438 (92.24%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGET-SGGGDG-EPSERLKRLS 60
           MA SISGLSLPS+FQY++S +AR  R RSF+IRSS DV GE  SGGGDG EPS R KRLS
Sbjct: 1   MAFSISGLSLPSVFQYESSILARKPRFRSFSIRSSNDVSGEVGSGGGDGSEPSNRPKRLS 60

Query: 61  EQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKV 120
           EQS+WEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLG+DSDIVFDYR KV
Sbjct: 61  EQSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKV 120

Query: 121 TRSFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKG 180
           TRSF +LQGDYYIAPVFM  +           VCHIVKNF+ HLLN+KVPLILG+WGGKG
Sbjct: 121 TRSFGHLQGDYYIAPVFMDKV-----------VCHIVKNFMVHLLNVKVPLILGVWGGKG 180

Query: 181 QGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMI 240
           QGKSFQTELIFQ MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMI
Sbjct: 181 QGKSFQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMI 240

Query: 241 NDIDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDF 300
           NDIDAGIGRFG+TQVTVNNQI +GTLMNLADNPTRVS+GQDWRE+DI NRIPIILTGNDF
Sbjct: 241 NDIDAGIGRFGSTQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADILNRIPIILTGNDF 300

Query: 301 STIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGA 360
           STIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISR+QV DIVN FPNQALDFYGA
Sbjct: 301 STIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVADIVNIFPNQALDFYGA 360

Query: 361 LRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQ 420
           LRSRTYDAA+SKWVDDIGGVEKLG+KLLKRRKNEKLPTF PPEQT+EALLEAGYSLVKEQ
Sbjct: 361 LRSRTYDAAISKWVDDIGGVEKLGEKLLKRRKNEKLPTFVPPEQTLEALLEAGYSLVKEQ 420

Query: 421 QLIMETKLSKEYMKNMDD 437
           +LIMETKLSKEYMKNMDD
Sbjct: 421 KLIMETKLSKEYMKNMDD 427

BLAST of MS009358 vs. ExPASy Swiss-Prot
Match: P93431 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RCA PE=1 SV=2)

HSP 1 Score: 325.5 bits (833), Expect = 9.3e-88
Identity = 176/345 (51.01%), Postives = 228/345 (66.09%), Query Frame = 0

Query: 93  GVIDDLF-AGNFLGRDSDIVFDYRHKV----TRSFEYLQGDYYIAPVFMLSLTCVNIVCV 152
           G +D LF A    G    ++  Y +      T  F+   G +YIAP FM  L        
Sbjct: 81  GFVDSLFQAPTGDGTHEAVLSSYEYLSQGLRTYDFDNTMGGFYIAPAFMDKL-------- 140

Query: 153 VDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESE 212
              V HI KNF+  L NIKVPLILGIWGGKGQGKSFQ EL+F  MG+ P++MSAGELES 
Sbjct: 141 ---VVHISKNFMT-LPNIKVPLILGIWGGKGQGKSFQCELVFAKMGINPIMMSAGELESG 200

Query: 213 RAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMN 272
            AGEP KLIR+RYR A+ +I+ +GK+ CL IND+DAG GR  G TQ TVNNQ+   TLMN
Sbjct: 201 NAGEPAKLIRQRYREAADIIK-KGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMN 260

Query: 273 LADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNI 332
           +ADNPT V +   + + D   R+PII+TGNDFST+YAPLIRDGRMEKFYW P R+D V +
Sbjct: 261 IADNPTNVQLPGMYNKED-NPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRVGV 320

Query: 333 VHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLL 392
              ++  D +    ++ IV++FP Q++DF+GALR+R YD  V KWV D  GVE +G +L+
Sbjct: 321 CKGIFRTDNVPDEDIVKIVDSFPGQSIDFFGALRARVYDDEVRKWVSDT-GVENIGKRLV 380

Query: 393 KRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 432
             R  E  P F  P+ T+E L+E GY LVKEQ+ +   +L+++Y+
Sbjct: 381 NSR--EGPPEFEQPKMTIEKLMEYGYMLVKEQENVKRVQLAEQYL 408

BLAST of MS009358 vs. ExPASy Swiss-Prot
Match: Q40565 (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RCA PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.3e-86
Identity = 189/407 (46.44%), Postives = 250/407 (61.43%), Query Frame = 0

Query: 46  GDGEPSERLKRLSEQSSWEAKDSEGRDYLYR-------LGNEADNMNIAVGARAGVIDDL 105
           G G  S ++   S + + E KD++ +   Y             D  +IA G   G++D L
Sbjct: 42  GKGVSSPKVTNRSLRIAAEEKDADPKKQTYSDRWKGLVQDFSDDQQDIARG--KGMVDSL 101

Query: 106 FAGNFLGRDSDIVFDYRHKVTRSFEYL-QG-----------DYYIAPVFMLSLTCVNIVC 165
           F        +       H V +S+EY+ QG            +YIAP FM  L       
Sbjct: 102 F-------QAPTGTGTHHAVLQSYEYVSQGLRQYNMDNTLDGFYIAPSFMDKL------- 161

Query: 166 VVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELES 225
               V HI KNF+  L NIKVPLILG+WGGKGQGKSFQ EL+F+ MG+ P++MSAGELES
Sbjct: 162 ----VVHITKNFL-KLPNIKVPLILGVWGGKGQGKSFQCELVFRKMGINPIMMSAGELES 221

Query: 226 ERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLM 285
             AGEP KLIR+RYR A+++I+ +G + CL IND+DAG GR  G TQ TVNNQ+   TLM
Sbjct: 222 GNAGEPAKLIRQRYREAAEIIR-KGNICCLFINDLDAGAGRMGGTTQYTVNNQMVNATLM 281

Query: 286 NLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVN 345
           N+ADNPT V +   + + +   R+PII+TGNDFST+YAPLIRDGRMEKFYW P RED + 
Sbjct: 282 NIADNPTNVQLPGMYNKQE-NARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIG 341

Query: 346 IVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKL 405
           +   ++  D +    VI IV+TFP Q++DF+GALR+R YD  V KWV    G+E +GDKL
Sbjct: 342 VCKGIFRTDNVPEEAVIKIVDTFPGQSIDFFGALRARVYDDEVRKWVSGT-GIEAIGDKL 401

Query: 406 LKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYMK 433
           L     +  PTF  P+ T+E LLE G  LV+EQ+ +   +L++ Y+K
Sbjct: 402 L--NSFDGPPTFEQPKMTVEKLLEYGNMLVQEQENVKRVQLAETYLK 422

BLAST of MS009358 vs. ExPASy Swiss-Prot
Match: O49074 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Solanum pennellii OX=28526 PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.3e-86
Identity = 199/441 (45.12%), Postives = 266/441 (60.32%), Query Frame = 0

Query: 1   MAISIS--GLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLS 60
           MA S+S  G +  +     NS         +F  +S K V     G    + S R  R+ 
Sbjct: 1   MAASVSTIGAASKAPLSLNNSVAGTSVPSTAFFGKSLKKV--YAKGVSSPKVSNRNLRVV 60

Query: 61  EQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLF-AGNFLGRDSDIVFDYRHK 120
            Q   E K+   +  LY      D  +IA G   G++D LF A    G    I+  Y + 
Sbjct: 61  AQEVDETKEDRWKG-LYD-NTSDDQQDIARG--KGLVDSLFQAPTGTGTHHAIMNSYEY- 120

Query: 121 VTRSFEYLQGD-----YYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILG 180
           V+++ +  Q D     +YIAP FM  L           V HI KNF+  L NIKVPLILG
Sbjct: 121 VSQALKTYQLDNKLDGFYIAPAFMDKL-----------VVHITKNFLT-LPNIKVPLILG 180

Query: 181 IWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGK 240
           +WGGKGQGKSFQ EL+F+ MG+ P++MSAGELES  AGEP KLIR+RYR A+++I+ +G 
Sbjct: 181 VWGGKGQGKSFQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAEIIR-KGN 240

Query: 241 LSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPI 300
           + CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + + +   R+PI
Sbjct: 241 MCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKQE-NARVPI 300

Query: 301 ILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQ 360
           I+TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D +    V+ IV++FP Q
Sbjct: 301 IVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEAVVKIVDSFPGQ 360

Query: 361 ALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAG 420
           ++DF+GALR+R YD  V KWV    G+E +G+KLL  R     PTF  P+ T+E LLE G
Sbjct: 361 SIDFFGALRARVYDDEVRKWVSGT-GIELIGEKLLNSRDGP--PTFEQPKMTLEKLLEYG 417

Query: 421 YSLVKEQQLIMETKLSKEYMK 433
             LV+EQ+ +   +L++ Y+K
Sbjct: 421 NMLVQEQENVKRVQLAETYLK 417

BLAST of MS009358 vs. ExPASy Swiss-Prot
Match: Q40460 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 3.9e-86
Identity = 183/395 (46.33%), Postives = 245/395 (62.03%), Query Frame = 0

Query: 51  SERLKRLSEQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDI 110
           ++ L+ ++EQ   + K     D    L  +  +    +    G++D LF        +  
Sbjct: 52  NKSLRIVAEQIDVDPKKQTDSDRWKGLVQDFSDDQQDITRGKGMVDSLF-------QAPT 111

Query: 111 VFDYRHKVTRSFEYL-QG-----------DYYIAPVFMLSLTCVNIVCVVDAVCHIVKNF 170
                H V +S+EY+ QG            +YIAP FM  L           V HI KNF
Sbjct: 112 GTGTHHAVLQSYEYVSQGLRQYNLDNKLDGFYIAPAFMDKL-----------VVHITKNF 171

Query: 171 IAHLLNIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRE 230
           +  L NIKVPLILGIWGGKGQGKSFQ EL+F+ MG+ P++MSAGELES  AGEP KLIR+
Sbjct: 172 L-KLPNIKVPLILGIWGGKGQGKSFQCELVFRKMGINPIMMSAGELESGNAGEPAKLIRQ 231

Query: 231 RYRTASQVIQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIG 290
           RYR A+++I+ +G + CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V + 
Sbjct: 232 RYREAAEIIR-KGNMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLP 291

Query: 291 QDWRESDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGIS 350
             + + +   R+PII+TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D + 
Sbjct: 292 GMYNKQE-NARVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCTGIFRTDNVP 351

Query: 351 RSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTF 410
              V+ IV+ FP Q++DF+GALR+R YD  V KWV    G+EK+GDKLL     +  PTF
Sbjct: 352 AEDVVKIVDNFPGQSIDFFGALRARVYDDEVRKWVSGT-GIEKIGDKLL--NSFDGPPTF 411

Query: 411 TPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYMK 433
             P+ T+E LLE G  LV+EQ+ +   +L+ +Y+K
Sbjct: 412 EQPKMTIEKLLEYGNMLVQEQENVKRVQLADKYLK 422

BLAST of MS009358 vs. ExPASy Swiss-Prot
Match: Q9ZT00 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Zea mays OX=4577 GN=RCA1 PE=2 SV=3)

HSP 1 Score: 317.4 bits (812), Expect = 2.5e-85
Identity = 182/397 (45.84%), Postives = 249/397 (62.72%), Query Frame = 0

Query: 42  TSGGGDGEPSERLKRLSEQSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAG 101
           T  G     + R K ++ +   E K ++  D    L  +  +    +    G++D+LF  
Sbjct: 36  TYHGKSSSSNSRFKAMAAKEVDETKQTD-EDRWKGLAYDISDDQQDITRGKGLVDNLFQA 95

Query: 102 NFLGRDSDIV----FDYRHKVTRS--FEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIV 161
             +G  + +     +DY  +  +S  F+ +   +YIA  FM  L           V H+ 
Sbjct: 96  P-MGDGTHVAVLSSYDYISQGQKSYNFDNMMDGFYIAKGFMDKL-----------VVHLS 155

Query: 162 KNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKL 221
           KNF+  L NIKVPLILGIWGGKGQGKSFQ EL+F  MG+ P++MSAGELES  AGEP KL
Sbjct: 156 KNFMT-LPNIKVPLILGIWGGKGQGKSFQCELVFAKMGITPIMMSAGELESGNAGEPAKL 215

Query: 222 IRERYRTASQVIQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRV 281
           IR+RYR AS +I+ +GK+SCL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V
Sbjct: 216 IRQRYREASDLIK-KGKMSCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNV 275

Query: 282 SIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKD 341
            +   + + D   R+PII+TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D
Sbjct: 276 QLPGMYNKED-NPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTD 335

Query: 342 GISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKL 401
           G+    V+ +V+TFP Q++DF+GALR+R YD  V +WV +  GVE +  KL+  +  E  
Sbjct: 336 GVDEEHVVQLVDTFPGQSIDFFGALRARVYDDEVRRWVSET-GVENIARKLVNSK--EGP 395

Query: 402 PTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYM 432
           PTF  P+ T+E LLE G+ LV EQ+ +   +L+ +Y+
Sbjct: 396 PTFEQPKITIEKLLEYGHMLVAEQENVKRVQLADKYL 413

BLAST of MS009358 vs. ExPASy TrEMBL
Match: A0A6J1DL15 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021429 PE=4 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.1e-236
Identity = 420/436 (96.33%), Postives = 422/436 (96.79%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAISISGLSLPSLFQYKNSTIAR+ RIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ
Sbjct: 1   MAISISGLSLPSLFQYKNSTIAREIRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR
Sbjct: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120

Query: 121 SFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180
           SFEYLQGDYYIAPVFM  +           VCHIVKNFIAHLLNIKVPLILGIWGGKGQG
Sbjct: 121 SFEYLQGDYYIAPVFMDKV-----------VCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180

Query: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240
           KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND
Sbjct: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240

Query: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300
           IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST
Sbjct: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300

Query: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360
           IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR
Sbjct: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360

Query: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420
           SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL
Sbjct: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420

Query: 421 IMETKLSKEYMKNMDD 437
           IMETKLSKEYMKNMDD
Sbjct: 421 IMETKLSKEYMKNMDD 425

BLAST of MS009358 vs. ExPASy TrEMBL
Match: A0A6J1H7W0 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111461246 PE=4 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.1e-216
Identity = 383/436 (87.84%), Postives = 405/436 (92.89%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+ISG+SLPSLF YKNS IAR +RIRSF+IRS KDV GE  GGG GEPS++ KRLSE+
Sbjct: 1   MAIAISGVSLPSLFHYKNSIIARKSRIRSFSIRSCKDVSGEGDGGG-GEPSQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLG+EADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGSEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180
           SFE+LQGDYYIAPVFM  +           VCHIVKNFI HLLNIKVPLILGIWGGKGQG
Sbjct: 121 SFEHLQGDYYIAPVFMDKV-----------VCHIVKNFIVHLLNIKVPLILGIWGGKGQG 180

Query: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240
           KSFQTELIFQ MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMIND
Sbjct: 181 KSFQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMIND 240

Query: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300
           IDAGIGRF NTQVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFST
Sbjct: 241 IDAGIGRFENTQVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFST 300

Query: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360
           IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALR
Sbjct: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALR 360

Query: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420
           SRTYD ++SKWVDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSL+KEQQ 
Sbjct: 361 SRTYDTSISKWVDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLIKEQQF 420

Query: 421 IMETKLSKEYMKNMDD 437
           +METKLSKEYMKNMDD
Sbjct: 421 VMETKLSKEYMKNMDD 424

BLAST of MS009358 vs. ExPASy TrEMBL
Match: A0A6J1KVM6 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497605 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.1e-216
Identity = 383/436 (87.84%), Postives = 403/436 (92.43%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQ 60
           MAI+IS +SLPSLFQYKN  IAR +RIRSF+IRS KDV GE  G G GEP ++ KRLSE+
Sbjct: 1   MAIAISAVSLPSLFQYKNLIIARKSRIRSFSIRSCKDVSGE--GDGGGEPLQKPKRLSEK 60

Query: 61  SSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTR 120
           SSWEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYR KVTR
Sbjct: 61  SSWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRQKVTR 120

Query: 121 SFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQG 180
           SFE+LQGDYYIAPVFM  +           VCHIVKNFI HLLNIKVPLILGIWGGKGQG
Sbjct: 121 SFEHLQGDYYIAPVFMDKV-----------VCHIVKNFIVHLLNIKVPLILGIWGGKGQG 180

Query: 181 KSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIND 240
           KSFQTELIFQ MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGKLSCLMIND
Sbjct: 181 KSFQTELIFQAMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKLSCLMIND 240

Query: 241 IDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFST 300
           IDAGIGRFGNTQVTVNNQI AGTLMNLADNP RVSIGQDWRE+DITNRIPIILTGNDFST
Sbjct: 241 IDAGIGRFGNTQVTVNNQIVAGTLMNLADNPNRVSIGQDWREADITNRIPIILTGNDFST 300

Query: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALR 360
           IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISR+QVIDIVNTFPNQALDFYGALR
Sbjct: 301 IYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVIDIVNTFPNQALDFYGALR 360

Query: 361 SRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQL 420
           SRTYD ++SKWVDDIGG+EKLG+KLLKRRKNE+LPTFTPPEQTM+ LLEAGYSLVKEQQ 
Sbjct: 361 SRTYDTSISKWVDDIGGMEKLGEKLLKRRKNEELPTFTPPEQTMDTLLEAGYSLVKEQQF 420

Query: 421 IMETKLSKEYMKNMDD 437
           +METKLSKEYMKNMDD
Sbjct: 421 VMETKLSKEYMKNMDD 423

BLAST of MS009358 vs. ExPASy TrEMBL
Match: A0A5A7UYP6 (Ribulose bisphosphate carboxylase/oxygenase activase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001550 PE=4 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 9.7e-213
Identity = 373/437 (85.35%), Postives = 402/437 (91.99%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDG-EPSERLKRLSE 60
           MA SISGLSLPS+F+YK+S +AR  R  SF++RSS DV  E   GGDG + S+R KRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVT 120
           QS+WEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLG+DSDIVFDYR KVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQ 180
           RSFE+LQGDYYIAP FM  +           VCHIVKNFI HLLN+KVPLILG+WGGKGQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKV-----------VCHIVKNFIVHLLNVKVPLILGVWGGKGQ 180

Query: 181 GKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIN 240
           GKSFQTELIFQ MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGK+SCLMIN
Sbjct: 181 GKSFQTELIFQMMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKMSCLMIN 240

Query: 241 DIDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFS 300
           DIDAGIGRFG TQVTVNNQI +GTLMNLADNPTRVS+GQDWRE+DI +RIPIILTGNDFS
Sbjct: 241 DIDAGIGRFGQTQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFS 300

Query: 301 TIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGAL 360
           TIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISR+QV+DIVNTFPNQALDFYGAL
Sbjct: 301 TIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGAL 360

Query: 361 RSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQ 420
           RSRTYD+A+SKWVDDIGGVEKLG+KLL+RRKNEKLPTFTPPEQT+EALL+AGYSLVKEQQ
Sbjct: 361 RSRTYDSAISKWVDDIGGVEKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQ 420

Query: 421 LIMETKLSKEYMKNMDD 437
           LIMETKLSKEYMKNMDD
Sbjct: 421 LIMETKLSKEYMKNMDD 426

BLAST of MS009358 vs. ExPASy TrEMBL
Match: A0A1S3CFE2 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103499863 PE=4 SV=1)

HSP 1 Score: 749.2 bits (1933), Expect = 9.7e-213
Identity = 373/437 (85.35%), Postives = 402/437 (91.99%), Query Frame = 0

Query: 1   MAISISGLSLPSLFQYKNSTIARDTRIRSFAIRSSKDVPGETSGGGDG-EPSERLKRLSE 60
           MA SISGLSLPS+F+YK+S +AR  R  SF++RSS DV  E   GGDG + S+R KRLSE
Sbjct: 1   MAFSISGLSLPSVFRYKSSILARKPRFHSFSVRSSNDVSSEVGSGGDGSDSSKRPKRLSE 60

Query: 61  QSSWEAKDSEGRDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVT 120
           QS+WEAKDSEG+DYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLG+DSDIVFDYR KVT
Sbjct: 61  QSTWEAKDSEGKDYLYRLGNEADNMNIAVGARAGVIDDLFAGNFLGKDSDIVFDYRQKVT 120

Query: 121 RSFEYLQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQ 180
           RSFE+LQGDYYIAP FM  +           VCHIVKNFI HLLN+KVPLILG+WGGKGQ
Sbjct: 121 RSFEHLQGDYYIAPTFMDKV-----------VCHIVKNFIVHLLNVKVPLILGVWGGKGQ 180

Query: 181 GKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMIN 240
           GKSFQTELIFQ MGVEPVIMSAGELESERAGEPGKLIRERYRTASQV+QNQGK+SCLMIN
Sbjct: 181 GKSFQTELIFQMMGVEPVIMSAGELESERAGEPGKLIRERYRTASQVVQNQGKMSCLMIN 240

Query: 241 DIDAGIGRFGNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFS 300
           DIDAGIGRFG TQVTVNNQI +GTLMNLADNPTRVS+GQDWRE+DI +RIPIILTGNDFS
Sbjct: 241 DIDAGIGRFGQTQVTVNNQIVSGTLMNLADNPTRVSVGQDWREADILHRIPIILTGNDFS 300

Query: 301 TIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGAL 360
           TIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISR+QV+DIVNTFPNQALDFYGAL
Sbjct: 301 TIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRAQVVDIVNTFPNQALDFYGAL 360

Query: 361 RSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQ 420
           RSRTYD+A+SKWVDDIGGVEKLG+KLL+RRKNEKLPTFTPPEQT+EALL+AGYSLVKEQQ
Sbjct: 361 RSRTYDSAISKWVDDIGGVEKLGEKLLRRRKNEKLPTFTPPEQTLEALLKAGYSLVKEQQ 420

Query: 421 LIMETKLSKEYMKNMDD 437
           LIMETKLSKEYMKNMDD
Sbjct: 421 LIMETKLSKEYMKNMDD 426

BLAST of MS009358 vs. TAIR 10
Match: AT1G73110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 606.7 bits (1563), Expect = 1.5e-173
Identity = 299/419 (71.36%), Postives = 349/419 (83.29%), Query Frame = 0

Query: 18  NSTIARDTRIRSFAIRSSKDVPGETSGGGDGEPSERLKRLSEQSSWEAKDSEGRDYLYRL 77
           N+T+    ++ S          G      DG    R K+LSEQSSWE KDSEG+DYLYRL
Sbjct: 26  NATLINTRKLSSIVCSKPSSNDGGKVANDDGGAKPR-KKLSEQSSWEVKDSEGKDYLYRL 85

Query: 78  GNEADNMNIAVGARAGVIDDLFAGNFLGRDSDIVFDYRHKVTRSFEYLQGDYYIAPVFML 137
           G E+DN+NIAVGAR+G+IDD+F G+FLG+DSDIVFDYR K TRSFE+LQGDYYIAP F+ 
Sbjct: 86  GAESDNVNIAVGARSGMIDDVFIGDFLGKDSDIVFDYRQKATRSFEHLQGDYYIAPSFLD 145

Query: 138 SLTCVNIVCVVDAVCHIVKNFIAHLLNIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPV 197
            +             HIVKN++A  LNIK+PLILGIWGGKGQGK+FQTELIF+TMGVEPV
Sbjct: 146 KV-----------AVHIVKNYLAPSLNIKIPLILGIWGGKGQGKTFQTELIFKTMGVEPV 205

Query: 198 IMSAGELESERAGEPGKLIRERYRTASQVIQNQGKLSCLMINDIDAGIGRFGNTQVTVNN 257
           IMSAGELES+RAGEPG+LIR+RYRTASQVIQNQGK+S LMINDIDAG+GRFG TQ+TVNN
Sbjct: 206 IMSAGELESDRAGEPGRLIRDRYRTASQVIQNQGKMSVLMINDIDAGLGRFGETQMTVNN 265

Query: 258 QIAAGTLMNLADNPTRVSIGQDWRESDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQ 317
           QI  GTLMNLADNPTRVS+GQ+WRE+D+ NR+P+I+TGNDFST+YAPLIR+GRMEKFYWQ
Sbjct: 266 QIVVGTLMNLADNPTRVSVGQEWREADMVNRVPLIVTGNDFSTLYAPLIREGRMEKFYWQ 325

Query: 318 PNREDIVNIVHRMYEKDGISRSQVIDIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGG 377
           P REDIVNIV RMYEKDGISR  VI IV+ FPNQALDFYGALRSRTYD ++ KWVD+ GG
Sbjct: 326 PTREDIVNIVSRMYEKDGISRKDVISIVDKFPNQALDFYGALRSRTYDRSILKWVDEAGG 385

Query: 378 VEKLGDKLLKRRKNEKLPTFTPPEQTMEALLEAGYSLVKEQQLIMETKLSKEYMKNMDD 437
           +E LG  LL+R+K +++P FT PEQT+EALLE+GYSL+ EQ+LIMETKLSKEYMKNMDD
Sbjct: 386 METLGKVLLRRKKTQEVPQFTAPEQTVEALLESGYSLINEQKLIMETKLSKEYMKNMDD 432

BLAST of MS009358 vs. TAIR 10
Match: AT2G39730.1 (rubisco activase )

HSP 1 Score: 308.9 bits (790), Expect = 6.4e-84
Identity = 167/329 (50.76%), Postives = 215/329 (65.35%), Query Frame = 0

Query: 116 HKVTRSFEY------------LQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLL 175
           H V  S+EY            +   +YIAP FM  L           V HI KNF+  L 
Sbjct: 106 HAVLSSYEYVSQGLRQYNLDNMMDGFYIAPAFMDKL-----------VVHITKNFLT-LP 165

Query: 176 NIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTA 235
           NIKVPLILGIWGGKGQGKSFQ EL+   MG+ P++MSAGELES  AGEP KLIR+RYR A
Sbjct: 166 NIKVPLILGIWGGKGQGKSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREA 225

Query: 236 SQVIQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRE 295
           + +I+ +GK+ CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + +
Sbjct: 226 ADLIK-KGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNK 285

Query: 296 SDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVI 355
            +   R+PII TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D I    ++
Sbjct: 286 EE-NARVPIICTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIV 345

Query: 356 DIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQ 415
            +V+ FP Q++DF+GALR+R YD  V K+V+ + GVEK+G +L+  R  E  P F  PE 
Sbjct: 346 TLVDQFPGQSIDFFGALRARVYDDEVRKFVESL-GVEKIGKRLVNSR--EGPPVFEQPEM 405

Query: 416 TMEALLEAGYSLVKEQQLIMETKLSKEYM 432
           T E L+E G  LV EQ+ +   +L++ Y+
Sbjct: 406 TYEKLMEYGNMLVMEQENVKRVQLAETYL 417

BLAST of MS009358 vs. TAIR 10
Match: AT2G39730.2 (rubisco activase )

HSP 1 Score: 308.9 bits (790), Expect = 6.4e-84
Identity = 167/329 (50.76%), Postives = 215/329 (65.35%), Query Frame = 0

Query: 116 HKVTRSFEY------------LQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLL 175
           H V  S+EY            +   +YIAP FM  L           V HI KNF+  L 
Sbjct: 106 HAVLSSYEYVSQGLRQYNLDNMMDGFYIAPAFMDKL-----------VVHITKNFLT-LP 165

Query: 176 NIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTA 235
           NIKVPLILGIWGGKGQGKSFQ EL+   MG+ P++MSAGELES  AGEP KLIR+RYR A
Sbjct: 166 NIKVPLILGIWGGKGQGKSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREA 225

Query: 236 SQVIQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRE 295
           + +I+ +GK+ CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + +
Sbjct: 226 ADLIK-KGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNK 285

Query: 296 SDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVI 355
            +   R+PII TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D I    ++
Sbjct: 286 EE-NARVPIICTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIV 345

Query: 356 DIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQ 415
            +V+ FP Q++DF+GALR+R YD  V K+V+ + GVEK+G +L+  R  E  P F  PE 
Sbjct: 346 TLVDQFPGQSIDFFGALRARVYDDEVRKFVESL-GVEKIGKRLVNSR--EGPPVFEQPEM 405

Query: 416 TMEALLEAGYSLVKEQQLIMETKLSKEYM 432
           T E L+E G  LV EQ+ +   +L++ Y+
Sbjct: 406 TYEKLMEYGNMLVMEQENVKRVQLAETYL 417

BLAST of MS009358 vs. TAIR 10
Match: AT2G39730.3 (rubisco activase )

HSP 1 Score: 308.9 bits (790), Expect = 6.4e-84
Identity = 167/329 (50.76%), Postives = 215/329 (65.35%), Query Frame = 0

Query: 116 HKVTRSFEY------------LQGDYYIAPVFMLSLTCVNIVCVVDAVCHIVKNFIAHLL 175
           H V  S+EY            +   +YIAP FM  L           V HI KNF+  L 
Sbjct: 106 HAVLSSYEYVSQGLRQYNLDNMMDGFYIAPAFMDKL-----------VVHITKNFLT-LP 165

Query: 176 NIKVPLILGIWGGKGQGKSFQTELIFQTMGVEPVIMSAGELESERAGEPGKLIRERYRTA 235
           NIKVPLILGIWGGKGQGKSFQ EL+   MG+ P++MSAGELES  AGEP KLIR+RYR A
Sbjct: 166 NIKVPLILGIWGGKGQGKSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREA 225

Query: 236 SQVIQNQGKLSCLMINDIDAGIGRF-GNTQVTVNNQIAAGTLMNLADNPTRVSIGQDWRE 295
           + +I+ +GK+ CL IND+DAG GR  G TQ TVNNQ+   TLMN+ADNPT V +   + +
Sbjct: 226 ADLIK-KGKMCCLFINDLDAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNK 285

Query: 296 SDITNRIPIILTGNDFSTIYAPLIRDGRMEKFYWQPNREDIVNIVHRMYEKDGISRSQVI 355
            +   R+PII TGNDFST+YAPLIRDGRMEKFYW P RED + +   ++  D I    ++
Sbjct: 286 EE-NARVPIICTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIV 345

Query: 356 DIVNTFPNQALDFYGALRSRTYDAAVSKWVDDIGGVEKLGDKLLKRRKNEKLPTFTPPEQ 415
            +V+ FP Q++DF+GALR+R YD  V K+V+ + GVEK+G +L+  R  E  P F  PE 
Sbjct: 346 TLVDQFPGQSIDFFGALRARVYDDEVRKFVESL-GVEKIGKRLVNSR--EGPPVFEQPEM 405

Query: 416 TMEALLEAGYSLVKEQQLIMETKLSKEYM 432
           T E L+E G  LV EQ+ +   +L++ Y+
Sbjct: 406 TYEKLMEYGNMLVMEQENVKRVQLAETYL 417