MS009355 (gene) Bitter gourd (TR) v1

BLAST of MS009355 vs. NCBI nr
Match: XP_022991872.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 964.5 bits (2492), Expect = 3.7e-277
Identity = 483/542 (89.11%), Postives = 508/542 (93.73%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MAVESNQT EFIFRSKLPDI+IPNHLPLH+YVFQ     GSRPCLINGATGD+YSY DV 
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           L SRRVAAGLH LGI KGDV+MNLLPNSPEFVF+FLGASY GAVMTAANPFYT  EIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+NAKLIVT ACFYDRVKDL   GVKIVCVDFA EGCLH+SVLS ADE H PPVE A 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQIDG+NPNL+YH  DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLRAGAAILIMQKFEI +LL+L+EK++VSIMPIVPPIFLAIAKSPE E YD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVLTMSLAFAK+PFAVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG IDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAV+GMPDEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEE+VKQFI+KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

BLAST of MS009355 vs. NCBI nr
Match: XP_038897021.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 961.1 bits (2483), Expect = 4.1e-276
Identity = 479/542 (88.38%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MA ESNQT EFIFRSKLPDI+IPNHLPLHDYVFQ  P   SRPCLINGATGDVYSY DVH
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLPKFASRPCLINGATGDVYSYHDVH 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           LT+RRVAAGLH LGI KGDV+MNLLPNSPEFVF+FLGASYRGA+MTAANPFYT  EIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+NAKLIVT ACFYDRVKDL   GVKIV VDFA EGCLHFSVLS ADE +APPV+ + 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVSVDFAVEGCLHFSVLSGADESNAPPVDFSP 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNL+YH DDVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHGDDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLR GA+I+IMQKF+I +LLQL+EKYR+SIMPIVPPIFLAIAKSPE E YD+S
Sbjct: 241 LNSILLCGLRVGASIMIMQKFDIVSLLQLIEKYRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPAAVLGQGYGMTEAGPVL+MSLAFAK+PF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLND ESTKRTIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG +DDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAV+GMPD +AGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VK NGGA+TEEEVKQFI+KQVVFYKRLKRVFFVN IPKAPSGKILRKELRA+LASGA
Sbjct: 481 AFVVKTNGGAITEEEVKQFISKQVVFYKRLKRVFFVNTIPKAPSGKILRKELRARLASGA 540

BLAST of MS009355 vs. NCBI nr
Match: KAG6593348.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 957.2 bits (2473), Expect = 6.0e-275
Identity = 480/542 (88.56%), Postives = 505/542 (93.17%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MA E NQT EFIFRSKLPDI+IPNHLPLH YVFQ +   GSRPCLINGATGDVYSY DV 
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           LT+RRVA+GLH LGI KGDV+MNLLPNSPEFVF+FLGASYRGA+MTAANPFYT  EIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+N KLIVT ACFYDRVKDL   GVKIVCVD+A E CLH+SVLS ADE H P V+ A 
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQIDGENPNL+YH  DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLRAGAAILIMQKFEI + LQLVEKY+VSIMPIVPPIFLAIAKSPELE YD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPELEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPAA+LGQGYGMTEAGPVLTMSLAFAK+PF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG IDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAV+GMPDEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEE+VKQFI+KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

BLAST of MS009355 vs. NCBI nr
Match: XP_023547628.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 955.7 bits (2469), Expect = 1.7e-274
Identity = 480/542 (88.56%), Postives = 505/542 (93.17%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MAVESNQT EFIFRSKLPDI+IPNHLPLH+YVFQ     GSRPCLINGATGDV+SY DV 
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVHSYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           L SRRVAAGLH LGI KGDV+MNLLPNSPEFVF+FL ASY GAVMTAANPFYT  EIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+NAKLIVT ACFYDRVKDL   GVKIVCVDFA EGCLH+SVLS ADE H P VE A 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQIDG+NPNL+YH  DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLRAGAAILIMQKFEI +LL+L+EK++VSIMPIVPPIFLAIAKSPE E YD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVLTMSLAFAK+PF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG IDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAV+GMPDEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEE+VKQFI+KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

BLAST of MS009355 vs. NCBI nr
Match: XP_022953208.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 954.5 bits (2466), Expect = 3.9e-274
Identity = 478/542 (88.19%), Postives = 504/542 (92.99%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MAVESNQT EFIFRSKLPDI+IPNHLPLH+YVFQ     GSRPCLINGATGDVYSY DV 
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           L SRRVAAGLH LGI K DV+MNLLPNSPEFVF+FL ASY GAVMTAANPFYT  EIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+NAKLIVT ACFYDRVKDL   GVKIVCVDFA EGCLH+SVLS ADE H P VE A 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQIDG+NPNL+YH  DVILCVLPFFH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLRAGAAILIMQKFEI +LL+L+EK++VSIMPIVPPIFLAIAKSPE E YD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVLTMSLAFAK+PF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG +DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAV+GMPDEQAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEE+VKQFI+KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

BLAST of MS009355 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 2.8e-235
Identity = 402/534 (75.28%), Postives = 459/534 (85.96%), Query Frame = 0

Query: 10  EFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVHLTSRRVAAG 69
           + IFRSKLPDIYIPNHLPLH Y F+      SRPCLINGA   +Y+Y DV L SR+VAAG
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQAKASNAKLI 129
           LHK GI   D IM LLPNSPEFVF+F+GASY GA+ T ANP +TPAE+ KQAKAS+AK+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTQACFYDRVKDL---GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAGDDVVALPY 189
           VTQAC  ++VKD      VKI+C+D APEGCLHFSVL+QA+E   P VEI  DDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGLVTSVAQQ+DGENPNL+ H++DV+LCVLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLSSVRVLKSG 309
           LR GAAILIMQKF+I + L+L+++Y+V+I P VPPI LAIAKSP +++YDLSSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGACGTVVRNAE 369
            APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAK+PF +K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGDIGLIDDDD 429
           MKIVDP+TG SLP N +GEICIRGDQIMKGYLNDPE+T RTIDKEGWL+TGDIG IDDDD
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPVAFAVKANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAVV M DEQAGEVPVAF V++NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 541
             +TE+EVK FI+KQV+FYKR+KRVFFV+AIPK+PSGKILRK+LRAKLA+G  N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of MS009355 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 6.9e-234
Identity = 398/543 (73.30%), Postives = 458/543 (84.35%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           M  E+NQ  + IFRSKLPDIYIP HLPLH Y F+      SRPCLINGA   +Y+Y DV 
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           LTSR+VAAGL+KLGI + D IM LLPNSPEFVF+F+GASY GA+ T ANP +TPAE+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKASNAKLIVTQACFYDRVKDL---GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIA 180
           AKASNAKLI+TQACF ++VKD      + ++C+D APEGC+HFS L+QADE   P V+I 
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 GDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQ+DGEN NL+ H++DV++CVLP FHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDL 300
           SLNS+LLCGLR GAAILIMQKF+I    +L+EKY+V+I P VPPI LAIAKSP ++NYDL
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGA 360
           SSVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAK+PF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLNDP +T RTIDKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVP 480
           DIG ID+DDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAVV M DEQAGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAF V++NG  +TE+EVK F++KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

BLAST of MS009355 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 9.1e-234
Identity = 395/540 (73.15%), Postives = 458/540 (84.81%), Query Frame = 0

Query: 4   ESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVHLTS 63
           E+ Q+ + IFRSKLPDIYIP HLPLH Y F+      SRPCLINGA   +Y+Y +V LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQAKA 123
           R+VA GL+KLGI + D IM LLPNSPEFVF+F+GASY GA+ T ANP +TPAE+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 SNAKLIVTQACFYDRVKDLGG---VKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAGDD 183
           S+AK+I+TQ+CF  +VKD      VK++C+D APEGCLHFS L+Q+DE   P V+I  DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQ+DGEN NL+ H++DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLSSV 303
           SILLCGLR GAAILIMQKF+I   L+L++KY+VSI P VPPI LAIAKSP +++YDLSSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAK+PF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLNDPE+T RTIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 LIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPVAF 483
            ID+DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAVV M DEQAGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 AVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 541
            V++NG A+TE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of MS009355 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 6.5e-232
Identity = 394/543 (72.56%), Postives = 456/543 (83.98%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           M +E+ Q+ + IFRSKLPDIYIP HLPLH Y F+      SRPCLI+GA   +Y+Y +V 
Sbjct: 3   MDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           LTSR+VA GL+KLGI + D IM LLPN PEFVF+F+GASY GA+ T ANP +TPAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKASNAKLIVTQACFYDRVKDL---GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIA 180
           AKAS+AK+++TQACF  +VKD      +K++CVD APEGC+HFS L Q+DE   P V+I 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 GDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQ+DGEN NL+ H+DDV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDL 300
           SLNS+LLC LR GAAILIMQKF+I   L+L+ K++V+I P VPPI LAIAKSP + NYDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGA 360
           SSVR + SG APLGKELEDAVRAKFP A LGQGYGMTEAGPVL M LAFAK+PF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLNDPE+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVP 480
           DIG IDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAVV M DEQAGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAF V++NG  +TE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

BLAST of MS009355 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-231
Identity = 393/543 (72.38%), Postives = 455/543 (83.79%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           M  E+ Q+ + IFRSKLPDIYIP HLPLH Y F+      SRPCLI+GA   +Y+Y +V 
Sbjct: 3   MDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVE 62

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           LTSR+VA GL+KLGI + D IM LLPN PEFVF+F+GASY GA+ T ANP +TPAE+ KQ
Sbjct: 63  LTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKASNAKLIVTQACFYDRVKDL---GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIA 180
           AKAS+AK+++TQACF  +VKD      +K++CVD  PEGC+HFS L Q+DE   P V+I 
Sbjct: 123 AKASSAKIVITQACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ 182

Query: 181 GDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIY 240
            DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQ+DGEN NL+ H+DDV++CVLP FHIY
Sbjct: 183 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDL 300
           SLNS+LLC LR GAAILIMQKF+I   L+L+ K++V+I P VPPI LAIAKSP ++NYDL
Sbjct: 243 SLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDL 302

Query: 301 SSVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGA 360
           SSVR + SG APLGKELEDAVRAKFP A LGQGYGMTEAGPVL M LAFAK+PF +K+GA
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTG 420
           CGTVVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLNDPE+T RTI+KEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTG 422

Query: 421 DIGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVP 480
           DIG IDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP +SDAAVV M DEQAGEVP
Sbjct: 423 DIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVP 482

Query: 481 VAFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASG 540
           VAF V++NG  +TE+EVK FI+KQV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 542

BLAST of MS009355 vs. ExPASy TrEMBL
Match: A0A6J1JN39 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488383 PE=4 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.8e-277
Identity = 483/542 (89.11%), Postives = 508/542 (93.73%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MAVESNQT EFIFRSKLPDI+IPNHLPLH+YVFQ     GSRPCLINGATGD+YSY DV 
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           L SRRVAAGLH LGI KGDV+MNLLPNSPEFVF+FLGASY GAVMTAANPFYT  EIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+NAKLIVT ACFYDRVKDL   GVKIVCVDFA EGCLH+SVLS ADE H PPVE A 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQIDG+NPNL+YH  DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLRAGAAILIMQKFEI +LL+L+EK++VSIMPIVPPIFLAIAKSPE E YD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVLTMSLAFAK+PFAVKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG IDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAV+GMPDEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEE+VKQFI+KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

BLAST of MS009355 vs. ExPASy TrEMBL
Match: A0A6J1GMS9 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455820 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 478/542 (88.19%), Postives = 504/542 (92.99%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MAVESNQT EFIFRSKLPDI+IPNHLPLH+YVFQ     GSRPCLINGATGDVYSY DV 
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           L SRRVAAGLH LGI K DV+MNLLPNSPEFVF+FL ASY GAVMTAANPFYT  EIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+NAKLIVT ACFYDRVKDL   GVKIVCVDFA EGCLH+SVLS ADE H P VE A 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQIDG+NPNL+YH  DVILCVLPFFH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLRAGAAILIMQKFEI +LL+L+EK++VSIMPIVPPIFLAIAKSPE E YD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVLTMSLAFAK+PF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRG+QIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG +DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAV+GMPDEQAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEE+VKQFI+KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

BLAST of MS009355 vs. ExPASy TrEMBL
Match: A0A6J1H7E7 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460855 PE=4 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.4e-274
Identity = 478/542 (88.19%), Postives = 505/542 (93.17%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MA E NQT EFIFRSKLPDI+IPNHLPLH YVFQ +   GSRPCLINGATGDVYSY DV 
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           LT+RRVA+GLH LGI KGDV+MNLLPNSPEFVF+FLGASYRGA+MTAANPFYT  EIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+N KLIVT ACFYDRVKDL   GVKIVCVD+A E CLH+SVLS ADE H P V+ A 
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQIDGENPNL+YH  DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLRAGAAILIMQKFEI + LQLVEKY+VSIMPIVPPIFLAIAKSP+LE YD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPAA+LGQGYGMTEAGPVLTMSLAFAK+PF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG ID+DDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAV+GMPDEQAGEVPV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEE+VKQFI+KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

BLAST of MS009355 vs. ExPASy TrEMBL
Match: A0A6J1KT15 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497966 PE=4 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.2e-273
Identity = 474/542 (87.45%), Postives = 506/542 (93.36%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MA E NQT EFIFRSKLPDI+IPNHLPLH YVFQ +  LGSRPCLINGATGDVYSY DV 
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           LT+RRVA+GLH LGI KG+V+MNLLPNSPEFVF+FLGASYRGA+MTAANPFYT  EIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+N KLIVT ACFYDRVKDL   GVKIVCVD+A EGCLH+SVLS ADE H P V+ A 
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQIDG+NPNL+YH  DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLRAGAAILIMQKFEI +LLQLVEKY+VSIMPIVPPIFLAIAKSP+LE YD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFPAA+LGQGYGMTEAGPVLTMSLAFAK+PF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG +DD++ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAV+GMPDE+AGEVPV
Sbjct: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEE+V QFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540

BLAST of MS009355 vs. ExPASy TrEMBL
Match: A0A5A7US61 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001380 PE=4 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.1e-272
Identity = 474/542 (87.45%), Postives = 502/542 (92.62%), Query Frame = 0

Query: 1   MAVESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVH 60
           MA ESNQT+EFIFRSKLPDI+IPNHLPLH YVFQ  P   SRPCLINGATGDVY+Y DV 
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQ 120
           LT+RRVAAGLH LGI KGDV+MNLLPNSPEFVF+FLGASYRGA+MTAANPFYT  EIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKASNAKLIVTQACFYDRVKDL--GGVKIVCVDFAPEGCLHFSVLSQADEDHAPPVEIAG 180
           AKA+NAKLIVT ACFYDRVKDL   GVKIVCVDFA EGCLHFSVLS ADE  AP V+ + 
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DGENPNL+YHADDVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLS 300
           LNSILLCGLR GA ILIMQKF+I +LLQL+EK+++SIMPIVPPIFLAIAKSPE E YD+S
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGAC 360
           SVRVLKSGGAPLGKELEDAVR KFP AVLGQGYGMTEAGPVL+MSLAFAK+PF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLND ESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPV 480
           IG +DDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAV+GMPD +AGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540
           AF VKANGGA+TEEEVKQFI KQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLA GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

BLAST of MS009355 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 774.6 bits (1999), Expect = 5.1e-224
Identity = 386/544 (70.96%), Postives = 450/544 (82.72%), Query Frame = 0

Query: 5   SNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVHLTSR 64
           +N   + IFRSKLPDIYIPNHL LHDY+FQ      ++PCLING TG VY+Y DVH+ SR
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQAKAS 124
           ++AA  HKLG+ + DV+M LLPN PEFV SFL AS+RGA  TAANPF+TPAEIAKQAKAS
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTQACFYDRVKDL---GGVKIVCVDF-----APEGCLHFSVLSQADEDHA---PP 184
           N KLI+T+A + D++K L    GV IVC+D       PEGCL F+ L+Q+  + +     
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VEIAGDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPF 244
           VEI+ DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQ+DGENPNL++H+DDVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELE 304
           FHIY+LNSI+LCGLR GAAILIM KFEI  LL+L+++ +V++ P+VPPI LAIAKS E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 NYDLSSVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAV 364
            YDLSS+RV+KSG APLGKELEDAV AKFP A LGQGYGMTEAGPVL MSL FAK+PF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+P +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQA 484
           LHTGDIGLIDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AVV M +E A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAK 538
           GEVPVAF VK+    ++E++VKQF++KQVVFYKR+ +VFF  +IPKAPSGKILRK+LRAK
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAK 556

BLAST of MS009355 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 755.4 bits (1949), Expect = 3.2e-218
Identity = 372/539 (69.02%), Postives = 451/539 (83.67%), Query Frame = 0

Query: 8   TREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVHLTSRRVA 67
           + + IFRS+LPDIYIPNHLPLHDY+F+      ++PCLING TG+VY+Y DVH+TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  AGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQAKASNAK 127
           AGLH LG+ + DV+M LLPNSPE V +FL AS+ GA+ T+ANPF+TPAEI+KQAKAS AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTQACFYDRVKDL--GGVKIVCV--DFAPEGCLHFSVLSQADEDHAP--PVEIAGDDV 187
           LIVTQ+ + D++K+L   GV IV    D  PE CL FS L+Q++E      P +I+ +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGLVTSVAQQ+DGENPNL+++ DDVILCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLSSVR 307
           I+LC LR GA ILIM KFEI  LL+ +++ +V++  +VPPI LAIAKSPE E YDLSSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGACGTV 367
           ++KSG APLGKELEDA+ AKFP A LGQGYGMTEAGPVL MSL FAK+PF VK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGDIGL 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLNDP +T  TIDK+GWLHTGD+G 
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 IDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPVAFA 487
           IDDDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AVV M +E AGEVPVAF 
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGAYN 541
           V++    ++E+E+KQF++KQVVFYKR+ +VFF ++IPKAPSGKILRK+LRA+LA+G  N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of MS009355 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 720.3 bits (1858), Expect = 1.1e-207
Identity = 359/509 (70.53%), Postives = 418/509 (82.12%), Query Frame = 0

Query: 5   SNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVHLTSR 64
           +N   + IFRSKLPDIYIPNHL LHDY+FQ      ++PCLING TG VY+Y DVH+ SR
Sbjct: 17  NNNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISR 76

Query: 65  RVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQAKAS 124
           ++AA  HKLG+ + DV+M LLPN PEFV SFL AS+RGA  TAANPF+TPAEIAKQAKAS
Sbjct: 77  QIAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKAS 136

Query: 125 NAKLIVTQACFYDRVKDL---GGVKIVCVDF-----APEGCLHFSVLSQADEDHA---PP 184
           N KLI+T+A + D++K L    GV IVC+D       PEGCL F+ L+Q+  + +     
Sbjct: 137 NTKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDS 196

Query: 185 VEIAGDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPF 244
           VEI+ DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQ+DGENPNL++H+DDVILCVLP 
Sbjct: 197 VEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPM 256

Query: 245 FHIYSLNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELE 304
           FHIY+LNSI+LCGLR GAAILIM KFEI  LL+L+++ +V++ P+VPPI LAIAKS E E
Sbjct: 257 FHIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETE 316

Query: 305 NYDLSSVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAV 364
            YDLSS+RV+KSG APLGKELEDAV AKFP A LGQGYGMTEAGPVL MSL FAK+PF V
Sbjct: 317 KYDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPV 376

Query: 365 KAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGW 424
           K+GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+P +T  TIDK+GW
Sbjct: 377 KSGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGW 436

Query: 425 LHTGDIGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQA 484
           LHTGDIGLIDDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AVV M +E A
Sbjct: 437 LHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAA 496

Query: 485 GEVPVAFAVKANGGAVTEEEVKQFIAKQV 503
           GEVPVAF VK+    ++E++VKQF++KQV
Sbjct: 497 GEVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of MS009355 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 694.5 bits (1791), Expect = 6.7e-200
Identity = 342/546 (62.64%), Postives = 427/546 (78.21%), Query Frame = 0

Query: 4   ESNQTREFIFRSKLPDIYIPNHLPLHDYVFQKKPNLG----SRPCLINGATGDVYSYDDV 63
           E   + +FIFRSKLPDI+IPNHLPL DYVFQ+    G    S  C+I+GATG + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  HLTSRRVAAGLHKLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAK 123
               RR+AAG+H+LGI  GDV+M LLPNSPEF  SFL  +Y GAV T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKASNAKLIVTQACFYDRVKDL--GGVKIVCVD---------FAPEGCLHFSVLSQADE 183
           QAKAS AK+I+T+ C  D++ +L   GV IVC+D          + +GC+ F+ L+QADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 DHAPPVEIAGDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVIL 243
                 +I+ +D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ++DGENPNL++ A+DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAK 303
           C LP FHIY+L++++L  +R GAA+LI+ +FE+  +++L+++Y+V+++P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPELENYDLSSVRVLKSGGAPLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAK 363
           SPE E YDLSSVR++ SG A L KELEDAVR KFP A+ GQGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 QPFAVKAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTI 423
            PF  K+GACGTV+RNAEMK+VD ETG SLP N +GEIC+RG Q+MKGYLNDPE+T RTI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGLIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGM 483
           DK+GWLHTGDIG +DDDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAVV M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDEQAGEVPVAFAVKANGGAVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRK 535
            DE A EVPVAF  ++ G  +TE++VK ++ KQVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of MS009355 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 686.0 bits (1769), Expect = 2.4e-197
Identity = 342/527 (64.90%), Postives = 422/527 (80.08%), Query Frame = 0

Query: 12  IFRSKLPDIYIPNHLPLHDYVFQKKPNLGSRPCLINGATGDVYSYDDVHLTSRRVAAGLH 71
           IFRSKLPDI IPNHLPLH Y F+K  ++  +PCLI G+TG  Y+Y + HL  RRVA+GL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  KLGIGKGDVIMNLLPNSPEFVFSFLGASYRGAVMTAANPFYTPAEIAKQAKASNAKLIVT 131
           KLGI KGDVIM LL NS EFVFSF+GAS  GAV T ANPFYT  E+ KQ K+S AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 QACFYDRVKDLG-GVKIVCVDF-APEGCLHFSVLSQADEDH--APPVEIAGDDVVALPYS 191
            + + D++K+LG  + ++  D   PE CL FS L   DE +     V+I GDD  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLVTSVAQQIDGENPNLHYHADDVILCVLPFFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK L+TSVAQQ+DG+NPNL+  ++DVILCVLP FHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RAGAAILIMQKFEIGTLLQLVEKYRVSIMPIVPPIFLAIAKSPELENYDLSSVRVLKSGG 311
           R+GA +L+M KFEIG LL L++++RV+I  +VPP+ +A+AK+P + +YDLSSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELEDAVRAKFPAAVLGQGYGMTEAGPVLTMSLAFAKQPFAVKAGACGTVVRNAEM 371
           APLGKEL+D++R + P A+LGQGYGMTEAGPVL+MSL FAK+P   K+G+CGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KIVDPETGASLPANSAGEICIRGDQIMKGYLNDPESTKRTIDKEGWLHTGDIGLIDDDDE 431
           K+V  ET  SL  N  GEICIRG QIMK YLNDPE+T  TID+EGWLHTGDIG +D+DDE
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVVGMPDEQAGEVPVAFAVKANGG 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAVV   DE AGEVPVAF V++NG 
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 AVTEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL 535
            +TEE+VK+++AKQVVFYKRL +VFFV +IPK+PSGKILRK+L+AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560