MS009330 (gene) Bitter gourd (TR) v1

Overview


Name	MS009330
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Siroheme synthase
Location	scaffold813: 329857 .. 332125 (+)
RNA-Seq Expression	MS009330
Synteny	MS009330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCGCGTTTACGACCTCCAATCGCTTTCATCGCCATTTTCGTCCCGCCCAAGAACACCCAGGACCCCAATTTTCAAACCCATCTCCTCAATCCACTGTTCCTCCTCTTCTTCCCCATTTACGGAGAAACATTCCATCAAGAGATACCAAAGAGACGATTGGGTGTACAAGAACCAAGCGGACCAAGCTTCAACTGCTCTGTCGTGTTCCGTTCCGTGCGATTCCGACTCAATACGGCAGGACGACATCGCTTTGCAGCTGCCGGAGCTGAAGAAGTTGCTGATGGTGCTGAGGGAGAAGAGGGTCAATGGTGGGTGCGATGATGGAGGAGAATGTGGGCCTGGGAATGTATTTCTAGTGGGGACTGGCCCTGGAGACCCTGAACTTTTGACATTGAAGGCTGTGAAAGTGATTCAGAGTGCCGATTTGCTTCTGTATGATCGTTTGGTGTCTAATGATGTGCTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTCGGCAAAACGGCAGGCTACCATAGCAGAACCCAGGTGAATGTTTCTTTGTGATTTTATCTATCTGAGTATTGAGTGTTTGAGTCTTGTTGCTGAATGGGAATTGGTTTTAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTAGTGAGACTTAAAGGAGGAGATCCTCTTGTAAGTGGAAACTTTGAATGGTTAAACTGCAATTGAATATGGTTTTGTCATTCAAGTGGTTTTTTTTAGTGGCATATCCTGAAATTTTTGTCATTGTACTTTATAGGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAAAAAGGGATTCAAGTAAAAATTGTTCCTGGTAAGCCTATTTCCGAATGCTGTTTCAGTCCTTTAAGTGTTTCCATGAAGCTTAACTTAATGTGTTCTGCATCACCATTTGTTTGAAAGATTTGGTTGTTTGAGGGTATTTTCATGACTAAAAGCAAATGACTTGAAGAGAATAGGTCCAAACCATGGTTGCCACCTACGTAAGATTTAATATCCTGAGTTTCTAGGTTTTAATATCCCGAGTTTCATTGACAATCAAATGTCATAGAATCAGAGTTAGAAAATTGTTTCATAGATTAGTCGAGGTTTGCACAACCTCAATGTCTGAAGGACGTGTATGGACCTGAACTACCGAGCCTTTATTTCTTTTTGAATCATGATACTGCCTCCTGTTTTGGATGGCAAAGTTTTCCTAATCTGTCAGCAGCCACAAGTAACATGAGTCTGAGGTTGATTGCTGATTACAATGTTGCTTGGCTTTGCTTCATGCTAGGAATCAGTAAATGAATTATCATGAATTTGCTGCATTTTGATTCTCAGTGTCACGTCTATGCACCACCATTTTAAGGAGAGTATTCTTAGAGTAACCTCATCTAAGAACACATGTGTTTGCCTTGCAGGTATAACTGCTGCTTCAGGTATAGCAGCTGAATTGGGGATTCCTTTAACGCACAGGGGCGTTGCAACGAACGTCAGGTTCCTCACTGGTCACTCTAGAAAGGGTGGAACGGATCCTCTATTTGTGGCAGAAAATGCTGCTGATCCGGATTCAACTCTGGTTGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCACGGTCTGCCACCCGATACCCCAGCTGCTGCCGTTGAACGAGGGACAACGCCCCAACAAAGAACTGTAAGCACAATTCTCTGTTGCTTTCTACTTTTGTCGGGCTTGGTTCAACCATATACAGCTTCTTCAAGAAGTCTCATGAGAGTCCTAATGCCTTGTCATTCCAGTTCTCAACTTTGCTCGACTTCTTTTGCGTTTCATCGATCGTTTTCTATGAGAAAGAAAAAAAAATCTTAAATGGGATCTGATCTATAGATTGCTCTCGAGTTCAGGAGACCTCTTGTAGGTCTATTTTCTTATCTGCGTTGCCTTCTGTACTTCATTAGCTATGTTCTCGAGAATGCCCGCTTCTAGACGTTTTTATCGAGGGGCGGGGCATGTTTGCTTGGCCTTTCATTTCATTTTGCTGGTGCCTATAACAAACTTTCCATGGATCTACTACAAATTATGCAGGTTTTTGCAGAACTGAAGGACCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTAATCATCATTGGAAAAGTGGTTTCTCTCTCACCGCATTGGTCTCTTTCTTCCAAAGAAGCCTCCTGTTTGGTGGAGGCT

mRNA sequence

ATGGCTCGCGTTTACGACCTCCAATCGCTTTCATCGCCATTTTCGTCCCGCCCAAGAACACCCAGGACCCCAATTTTCAAACCCATCTCCTCAATCCACTGTTCCTCCTCTTCTTCCCCATTTACGGAGAAACATTCCATCAAGAGATACCAAAGAGACGATTGGGTGTACAAGAACCAAGCGGACCAAGCTTCAACTGCTCTGTCGTGTTCCGTTCCGTGCGATTCCGACTCAATACGGCAGGACGACATCGCTTTGCAGCTGCCGGAGCTGAAGAAGTTGCTGATGGTGCTGAGGGAGAAGAGGGTCAATGGTGGGTGCGATGATGGAGGAGAATGTGGGCCTGGGAATGTATTTCTAGTGGGGACTGGCCCTGGAGACCCTGAACTTTTGACATTGAAGGCTGTGAAAGTGATTCAGAGTGCCGATTTGCTTCTGTATGATCGTTTGGTGTCTAATGATGTGCTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTCGGCAAAACGGCAGGCTACCATAGCAGAACCCAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTAGTGAGACTTAAAGGAGGAGATCCTCTTGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAAAAAGGGATTCAAGTAAAAATTGTTCCTGGTATAACTGCTGCTTCAGGTATAGCAGCTGAATTGGGGATTCCTTTAACGCACAGGGGCGTTGCAACGAACGTCAGGTTCCTCACTGGTCACTCTAGAAAGGGTGGAACGGATCCTCTATTTGTGGCAGAAAATGCTGCTGATCCGGATTCAACTCTGGTTGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCACGGTCTGCCACCCGATACCCCAGCTGCTGCCGTTGAACGAGGGACAACGCCCCAACAAAGAACTGTTTTTGCAGAACTGAAGGACCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTAATCATCATTGGAAAAGTGGTTTCTCTCTCACCGCATTGGTCTCTTTCTTCCAAAGAAGCCTCCTGTTTGGTGGAGGCT

Coding sequence (CDS)

ATGGCTCGCGTTTACGACCTCCAATCGCTTTCATCGCCATTTTCGTCCCGCCCAAGAACACCCAGGACCCCAATTTTCAAACCCATCTCCTCAATCCACTGTTCCTCCTCTTCTTCCCCATTTACGGAGAAACATTCCATCAAGAGATACCAAAGAGACGATTGGGTGTACAAGAACCAAGCGGACCAAGCTTCAACTGCTCTGTCGTGTTCCGTTCCGTGCGATTCCGACTCAATACGGCAGGACGACATCGCTTTGCAGCTGCCGGAGCTGAAGAAGTTGCTGATGGTGCTGAGGGAGAAGAGGGTCAATGGTGGGTGCGATGATGGAGGAGAATGTGGGCCTGGGAATGTATTTCTAGTGGGGACTGGCCCTGGAGACCCTGAACTTTTGACATTGAAGGCTGTGAAAGTGATTCAGAGTGCCGATTTGCTTCTGTATGATCGTTTGGTGTCTAATGATGTGCTGGATTTGGTGGGTCCTGATGCTAGGCTTCTCTATGTCGGCAAAACGGCAGGCTACCATAGCAGAACCCAGGAGGAGATTCATGAGTTACTTCTGAACTTTGCTGAAGCTGGAGCTACAGTAGTGAGACTTAAAGGAGGAGATCCTCTTGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAAAAAGGGATTCAAGTAAAAATTGTTCCTGGTATAACTGCTGCTTCAGGTATAGCAGCTGAATTGGGGATTCCTTTAACGCACAGGGGCGTTGCAACGAACGTCAGGTTCCTCACTGGTCACTCTAGAAAGGGTGGAACGGATCCTCTATTTGTGGCAGAAAATGCTGCTGATCCGGATTCAACTCTGGTTGTATATATGGGTCTGTCAACTCTTCCATCTCTGGCCCTTAAGTTGATGCATCACGGTCTGCCACCCGATACCCCAGCTGCTGCCGTTGAACGAGGGACAACGCCCCAACAAAGAACTGTTTTTGCAGAACTGAAGGACCTTGCAGATGAAATCAAAGCAGCAGAGTTGGTTTCACCTACTTTAATCATCATTGGAAAAGTGGTTTCTCTCTCACCGCATTGGTCTCTTTCTTCCAAAGAAGCCTCCTGTTTGGTGGAGGCT

Protein sequence

MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASCLVEA

Homology

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BLAST of MS009330 vs. NCBI nr
Match: XP_022149015.1 (uncharacterized protein LOC111017535 [Momordica charantia])

HSP 1 Score: 724.9 bits (1870), Expect = 3.4e-205
Identity = 367/369 (99.46%), Postives = 368/369 (99.73%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ
Sbjct: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFL 120
           ADQASTALSCSVPCDSDSIRQDDIALQLPELK+LLMVLREKRVNGGCDDGGECGPGNVFL
Sbjct: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAAD DSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEASCLVEA
Sbjct: 361 KEASCLVEA 369

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BLAST of MS009330 vs. NCBI nr
Match: XP_022991905.1 (uncharacterized protein LOC111488402 [Cucurbita maxima])

HSP 1 Score: 661.4 bits (1705), Expect = 4.6e-186
Identity = 337/369 (91.33%), Postives = 351/369 (95.12%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARV DLQSLSS FSSRP  PR+  FKPI S HCSSSSSPFTEKHS+KRYQRDDWVYKNQ
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWVYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFL 120
           AD++S A SCS+ CDS+SIRQ+DIALQLPELKKLL VLREKR + GCDD G+CGPGNVFL
Sbjct: 61  ADESSVASSCSISCDSESIRQNDIALQLPELKKLLQVLREKRASSGCDD-GKCGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEAS LVEA
Sbjct: 361 KEASSLVEA 368

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BLAST of MS009330 vs. NCBI nr
Match: XP_023548332.1 (uncharacterized protein LOC111807000 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 658.3 bits (1697), Expect = 3.9e-185
Identity = 335/369 (90.79%), Postives = 351/369 (95.12%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARV DLQSLSS F+SRP  PR+  FKPI S HCSSSSSPFTEKHS+KRYQRDDW+YKNQ
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWLYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFL 120
           AD++S A SCS+P DS+SIRQ+DIALQLPELKKLL VLREKR + GCDD G+CGPGNVFL
Sbjct: 61  ADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGCDD-GKCGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEAS LVEA
Sbjct: 361 KEASSLVEA 368

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BLAST of MS009330 vs. NCBI nr
Match: XP_008461254.1 (PREDICTED: uroporphyrinogen-III C-methyltransferase [Cucumis melo])

HSP 1 Score: 654.8 bits (1688), Expect = 4.3e-184
Identity = 335/373 (89.81%), Postives = 350/373 (93.83%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60
           MAR  DLQSLSSPFSS P  PR+P FKPI S HC    SSSSSPFTEKHSIKRYQRDDW+
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSYSSSSSSSPFTEKHSIKRYQRDDWL 60

Query: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPG 120
           YKNQ+DQ S   SCS+P DS+S+RQ+DIA+QLPELKKLL VLREKRV+ GCDD G+CGPG
Sbjct: 61  YKNQSDQPSVTSSCSIPYDSESMRQNDIAMQLPELKKLLEVLREKRVSSGCDD-GKCGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHS
Sbjct: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240

Query: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK 300
           AAELGIPLTHRGVAT+VRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFA LKDLADEIKAAELVSPTLI+IG+VVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAHLKDLADEIKAAELVSPTLIVIGRVVSLSPHW 360

Query: 361 SLSSKEASCLVEA 370
           SLSS EAS LVEA
Sbjct: 361 SLSSNEASSLVEA 372

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BLAST of MS009330 vs. NCBI nr
Match: XP_004135909.1 (uncharacterized protein LOC101222061 [Cucumis sativus] >KGN45161.1 hypothetical protein Csa_016214 [Cucumis sativus])

HSP 1 Score: 650.2 bits (1676), Expect = 1.1e-182
Identity = 332/373 (89.01%), Postives = 350/373 (93.83%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60
           MAR  DLQSLSSPFSS P  PR+P FKPI S HC    SSSSSPFTEKHS+KRYQRDDW+
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSSASSSSSSPFTEKHSVKRYQRDDWL 60

Query: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPG 120
           YK Q+DQ S   SCS+P DS+SIRQ+DIA+QLPELKKLL VLREKRV+ GCDD G+CGPG
Sbjct: 61  YKYQSDQPSVTSSCSIPYDSESIRQNDIAMQLPELKKLLEVLREKRVSNGCDD-GKCGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           +VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHS
Sbjct: 121 DVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240

Query: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK 300
           AAELGIPLTHRGVAT+VRFLTGHSRKGGTDPL+VAENAADPDSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFA+LKDLADEIKAAELVSPTLI+IG+VVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAQLKDLADEIKAAELVSPTLIVIGRVVSLSPHW 360

Query: 361 SLSSKEASCLVEA 370
           SLSS EAS LVEA
Sbjct: 361 SLSSNEASSLVEA 372

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BLAST of MS009330 vs. ExPASy Swiss-Prot
Match: Q42606 (S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=UPM1 PE=1 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.3e-148
Identity = 275/344 (79.94%), Postives = 300/344 (87.21%), Query Frame = 0


Query: 28  PISSIH---CSSSSSPFTEKHSIKRYQRDDWVYKNQADQASTALSCSVPCDSDSIRQDDI 87
           PI  +H    SSSSSPFTEKHS++RYQRD W+YK       +  S S   D   +R++DI
Sbjct: 26  PICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVRENDI 85

Query: 88  ALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADL 147
           A QLPELKKLL VL+EKRV  GC  GG+CGPG+V+LVGTGPGDPELLTLKAV+VIQSADL
Sbjct: 86  ASQLPELKKLLAVLKEKRVK-GC-KGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQSADL 145

Query: 148 LLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 207
           LLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP
Sbjct: 146 LLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 205

Query: 208 LVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGG 267
           LVFGRGGEEMDFLQQ+GI+V+++PGITAASGIAAELGIPLTHRGVAT+VRFLTGHSRKGG
Sbjct: 206 LVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRKGG 265

Query: 268 TDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFA 327
           TDPLFVAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRTVFA
Sbjct: 266 TDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRTVFA 325

Query: 328 ELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASCLVE 369
           ELKD A EI++A LVSPTLIIIGKVV LSP W   +KE+SCLVE
Sbjct: 326 ELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVE 367

[Close]

BLAST of MS009330 vs. ExPASy Swiss-Prot
Match: B8GUD3 (Siroheme synthase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=cysG PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.7e-61
Identity = 127/255 (49.80%), Postives = 169/255 (66.27%), Query Frame = 0


Query: 100 EKRVNGGCD--DGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLD 159
           EK +  G D  D G    G VFLVG GPGDP+LLT +A++++Q AD+++YD LVS  +++
Sbjct: 204 EKALETGLDTRDAG----GEVFLVGAGPGDPDLLTFRALRLMQLADVVVYDNLVSPAIIE 263

Query: 160 LVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFL 219
           LV  DA ++Y GK    H+  QEEI++LL+  A+ G  V+RLKGGDP +FGRGGEE+D L
Sbjct: 264 LVRRDAEMIYAGKKRNLHTLPQEEINQLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIDTL 323

Query: 220 QQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADP 279
            Q+GI  ++VPGITAA+G A+  GIPLTHR  A  V F TGH R G  D  +  +  A P
Sbjct: 324 MQEGIPFQVVPGITAAAGCASFSGIPLTHRDYAQAVVFATGHLRDGSIDLNW--KMLAQP 383

Query: 280 DSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAE 339
             T+V YMGL  LP +  +LM HG+ PD P A VE+GTT  QR +   L  + D +K  +
Sbjct: 384 RQTVVFYMGLLGLPIICRELMAHGVSPDMPMALVEQGTTQNQRVIVGTLASMPDLVKDYD 443

Query: 340 LVSPTLIIIGKVVSL 353
           +  PTLII+G+VV L
Sbjct: 444 VQPPTLIIVGEVVKL 452

[Close]

BLAST of MS009330 vs. ExPASy Swiss-Prot
Match: Q6F8G6 (Siroheme synthase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=cysG PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 8.2e-61
Identity = 124/237 (52.32%), Postives = 162/237 (68.35%), Query Frame = 0


Query: 116 GNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYH 175
           G V+LVG GPGDPELLTLKA++++Q AD+++YDRLVS  +L+L   DA  +YVGK    H
Sbjct: 216 GEVYLVGAGPGDPELLTLKALRLMQQADVVIYDRLVSAPILELCRRDAEKVYVGKARSNH 275

Query: 176 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASG 235
           S  QE I+ LL+ +A+AG  V RLKGGDP +FGRGGEE+  L   GI  ++VPGITAASG
Sbjct: 276 SVPQEGINALLVKYAQAGKRVCRLKGGDPFIFGRGGEEIQELFAAGIPFQVVPGITAASG 335

Query: 236 IAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLAL 295
            +A  GIPLTHR  A +VRFLTGH ++G   P          + TLV+YMGL  L  +  
Sbjct: 336 CSAYAGIPLTHRDYAQSVRFLTGHLKEG--SPELPWSELVYENQTLVLYMGLVGLEHICQ 395

Query: 296 KLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSL 353
           +L+ HG  PD P A V +GTTP+Q+ V   L ++A +I   ++ +PTL IIG+VVSL
Sbjct: 396 QLIAHGQRPDMPVALVSKGTTPEQKVVVGTLSNIASKIAEYQIHAPTLTIIGEVVSL 450

[Close]

BLAST of MS009330 vs. ExPASy Swiss-Prot
Match: Q0VQ05 (Siroheme synthase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2) OX=393595 GN=cysG PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 8.2e-61
Identity = 120/239 (50.21%), Postives = 161/239 (67.36%), Query Frame = 0


Query: 116 GNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYH 175
           G V+LVG GPGDP+LLT +A++++Q AD++LYDRLV   ++DL   DA L+YVGK    H
Sbjct: 218 GEVYLVGAGPGDPDLLTFRALRLLQKADVVLYDRLVGKGIVDLARRDAELVYVGKARDKH 277

Query: 176 SRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASG 235
           +  Q+ I+ELL+++A+ G  V RLKGGDP +FGRGGEE+D +  +GI  ++VPGITAASG
Sbjct: 278 ALPQDNINELLVHYAKQGKKVCRLKGGDPFIFGRGGEEIDLIVAEGIDFQVVPGITAASG 337

Query: 236 IAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLAL 295
            A+  GIPLTHR  A +VRF+TGH + G  D     ++      T+V YMGL  L  +  
Sbjct: 338 CASYAGIPLTHRDHAQSVRFVTGHRKDGSVD--LDWKHLVSETETVVFYMGLVGLREICS 397

Query: 296 KLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSP 355
           +L+ HG   DTP A V RGTT  Q  +   L  L D+I+  E+ +PTLII+G VVSL P
Sbjct: 398 QLIAHGRGGDTPIALVSRGTTNLQEVITGRLDQLPDDIEGREIHAPTLIIVGSVVSLHP 454

[Close]

BLAST of MS009330 vs. ExPASy Swiss-Prot
Match: Q02NA3 (Siroheme synthase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) OX=208963 GN=cysG PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.4e-60
Identity = 125/255 (49.02%), Postives = 168/255 (65.88%), Query Frame = 0


Query: 98  LREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLD 157
           L E+R+ GG         G V+LVG GPGDP+LLT +A++++Q AD++LYDRLV+  +L+
Sbjct: 202 LLEERLAGGL---AHIATGEVYLVGAGPGDPDLLTFRALRLMQQADVVLYDRLVAPSILE 261

Query: 158 LVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFL 217
           L   DA  LYVGK    H+  Q+ I+ LL+  A  G  V+RLKGGDP +FGRGGEE+D L
Sbjct: 262 LCRRDAERLYVGKRRAEHAVPQDRINRLLVELASQGKRVLRLKGGDPFIFGRGGEEIDEL 321

Query: 218 QQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADP 277
             +GI  ++VPGITAASG AA  GIPLTHR  A +VRF+TGH + G TD     ++   P
Sbjct: 322 AARGIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGHLKDGTTD--LPWQDLVAP 381

Query: 278 DSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAE 337
             TLV YMGL  LP +  +L+ HG    TPAA +++GTT QQR     L++L   +   E
Sbjct: 382 GQTLVFYMGLVGLPVICEQLVAHGRSAQTPAALIQQGTTAQQRVFTGTLENLPQLVAEHE 441

Query: 338 LVSPTLIIIGKVVSL 353
           + +PTL+I+G+VV L
Sbjct: 442 VHAPTLVIVGEVVQL 451

[Close]

BLAST of MS009330 vs. ExPASy TrEMBL
Match: A0A6J1D4K3 (uncharacterized protein LOC111017535 OS=Momordica charantia OX=3673 GN=LOC111017535 PE=3 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 1.7e-205
Identity = 367/369 (99.46%), Postives = 368/369 (99.73%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ
Sbjct: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFL 120
           ADQASTALSCSVPCDSDSIRQDDIALQLPELK+LLMVLREKRVNGGCDDGGECGPGNVFL
Sbjct: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKRLLMVLREKRVNGGCDDGGECGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAAD DSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADLDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEASCLVEA
Sbjct: 361 KEASCLVEA 369

[Close]

BLAST of MS009330 vs. ExPASy TrEMBL
Match: A0A6J1JU84 (uncharacterized protein LOC111488402 OS=Cucurbita maxima OX=3661 GN=LOC111488402 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.2e-186
Identity = 337/369 (91.33%), Postives = 351/369 (95.12%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHCSSSSSPFTEKHSIKRYQRDDWVYKNQ 60
           MARV DLQSLSS FSSRP  PR+  FKPI S HCSSSSSPFTEKHS+KRYQRDDWVYKNQ
Sbjct: 1   MARVCDLQSLSSLFSSRPTIPRSSNFKPICSFHCSSSSSPFTEKHSVKRYQRDDWVYKNQ 60

Query: 61  ADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFL 120
           AD++S A SCS+ CDS+SIRQ+DIALQLPELKKLL VLREKR + GCDD G+CGPGNVFL
Sbjct: 61  ADESSVASSCSISCDSESIRQNDIALQLPELKKLLQVLREKRASSGCDD-GKCGPGNVFL 120

Query: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQE 180
           VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG+HSRTQE
Sbjct: 121 VGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAGFHSRTQE 180

Query: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAEL 240
           EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGIAAEL
Sbjct: 181 EIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGIAAEL 240

Query: 241 GIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300
           GIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH
Sbjct: 241 GIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHH 300

Query: 301 GLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360
           GLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS
Sbjct: 301 GLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSS 360

Query: 361 KEASCLVEA 370
           KEAS LVEA
Sbjct: 361 KEASSLVEA 368

[Close]

BLAST of MS009330 vs. ExPASy TrEMBL
Match: A0A1S3CDT8 (uroporphyrinogen-III C-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499894 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.1e-184
Identity = 335/373 (89.81%), Postives = 350/373 (93.83%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60
           MAR  DLQSLSSPFSS P  PR+P FKPI S HC    SSSSSPFTEKHSIKRYQRDDW+
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSYSSSSSSSPFTEKHSIKRYQRDDWL 60

Query: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPG 120
           YKNQ+DQ S   SCS+P DS+S+RQ+DIA+QLPELKKLL VLREKRV+ GCDD G+CGPG
Sbjct: 61  YKNQSDQPSVTSSCSIPYDSESMRQNDIAMQLPELKKLLEVLREKRVSSGCDD-GKCGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHS
Sbjct: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240

Query: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK 300
           AAELGIPLTHRGVAT+VRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATSVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFA LKDLADEIKAAELVSPTLI+IG+VVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAHLKDLADEIKAAELVSPTLIVIGRVVSLSPHW 360

Query: 361 SLSSKEASCLVEA 370
           SLSS EAS LVEA
Sbjct: 361 SLSSNEASSLVEA 372

[Close]

BLAST of MS009330 vs. ExPASy TrEMBL
Match: A0A0A0KBI8 (TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428980 PE=3 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 5.2e-183
Identity = 332/373 (89.01%), Postives = 350/373 (93.83%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC----SSSSSPFTEKHSIKRYQRDDWV 60
           MAR  DLQSLSSPFSS P  PR+P FKPI S HC    SSSSSPFTEKHS+KRYQRDDW+
Sbjct: 1   MARFCDLQSLSSPFSSHPTIPRSPNFKPIFSFHCSSASSSSSSPFTEKHSVKRYQRDDWL 60

Query: 61  YKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGECGPG 120
           YK Q+DQ S   SCS+P DS+SIRQ+DIA+QLPELKKLL VLREKRV+ GCDD G+CGPG
Sbjct: 61  YKYQSDQPSVTSSCSIPYDSESIRQNDIAMQLPELKKLLEVLREKRVSNGCDD-GKCGPG 120

Query: 121 NVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAGYHS 180
           +VFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVL+LVGPDARLLYVGKTAGYHS
Sbjct: 121 DVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLELVGPDARLLYVGKTAGYHS 180

Query: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAASGI 240
           RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAASGI
Sbjct: 181 RTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAASGI 240

Query: 241 AAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSLALK 300
           AAELGIPLTHRGVAT+VRFLTGHSRKGGTDPL+VAENAADPDSTLVVYMGLSTLPSLALK
Sbjct: 241 AAELGIPLTHRGVATSVRFLTGHSRKGGTDPLYVAENAADPDSTLVVYMGLSTLPSLALK 300

Query: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLSPHW 360
           LMHHGLPPDTPAAAVERGTTPQQRTVFA+LKDLADEIKAAELVSPTLI+IG+VVSLSPHW
Sbjct: 301 LMHHGLPPDTPAAAVERGTTPQQRTVFAQLKDLADEIKAAELVSPTLIVIGRVVSLSPHW 360

Query: 361 SLSSKEASCLVEA 370
           SLSS EAS LVEA
Sbjct: 361 SLSSNEASSLVEA 372

[Close]

BLAST of MS009330 vs. ExPASy TrEMBL
Match: A0A6J1GQM1 (uncharacterized protein LOC111456604 OS=Cucurbita moschata OX=3662 GN=LOC111456604 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.7e-182
Identity = 334/376 (88.83%), Postives = 350/376 (93.09%), Query Frame = 0


Query: 1   MARVYDLQSLSSPFSSRPRTPRTPIFKPISSIHC-------SSSSSPFTEKHSIKRYQRD 60
           MARV DLQSLSS F+SRP  PR+  FKPI S HC       SSSSSPFTEKHS+KRYQRD
Sbjct: 1   MARVCDLQSLSSLFASRPTIPRSSNFKPICSFHCSSSSSSSSSSSSPFTEKHSVKRYQRD 60

Query: 61  DWVYKNQADQASTALSCSVPCDSDSIRQDDIALQLPELKKLLMVLREKRVNGGCDDGGEC 120
           DW+YKNQAD++S A SCS+P DS+SIRQ+DIALQLPELKKLL VLREKR + G DD G+C
Sbjct: 61  DWLYKNQADESSVASSCSIPSDSESIRQNDIALQLPELKKLLQVLREKRASSGGDD-GKC 120

Query: 121 GPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGPDARLLYVGKTAG 180
           GPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVG DARLLYVGKTAG
Sbjct: 121 GPGNVFLVGTGPGDPELLTLKAVKVIQSADLLLYDRLVSNDVLDLVGSDARLLYVGKTAG 180

Query: 181 YHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQKGIQVKIVPGITAA 240
           +HSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQ+GIQVKIVPGITAA
Sbjct: 181 FHSRTQEEIHELLLNFAEAGATVVRLKGGDPLVFGRGGEEMDFLQQQGIQVKIVPGITAA 240

Query: 241 SGIAAELGIPLTHRGVATNVRFLTGHSRKGGTDPLFVAENAADPDSTLVVYMGLSTLPSL 300
           SGIAAELGIPLTHRGVAT+VRFLTGHSR+GGTDPLFVAENAADPDSTLVVYMGLSTLPSL
Sbjct: 241 SGIAAELGIPLTHRGVATSVRFLTGHSRQGGTDPLFVAENAADPDSTLVVYMGLSTLPSL 300

Query: 301 ALKLMHHGLPPDTPAAAVERGTTPQQRTVFAELKDLADEIKAAELVSPTLIIIGKVVSLS 360
           ALKLMHHGLPPDTPAAA+ERGTTPQQR VFAELKDLADEIKAAELVSPTLIIIGKVVSLS
Sbjct: 301 ALKLMHHGLPPDTPAAAIERGTTPQQRIVFAELKDLADEIKAAELVSPTLIIIGKVVSLS 360

Query: 361 PHWSLSSKEASCLVEA 370
           PHWSLSSKEAS LVEA
Sbjct: 361 PHWSLSSKEASSLVEA 375

[Close]

BLAST of MS009330 vs. TAIR 10
Match: AT5G40850.1 (urophorphyrin methylase 1 )

HSP 1 Score: 526.6 bits (1355), Expect = 1.7e-149
Identity = 275/344 (79.94%), Postives = 300/344 (87.21%), Query Frame = 0


Query: 28  PISSIH---CSSSSSPFTEKHSIKRYQRDDWVYKNQADQASTALSCSVPCDSDSIRQDDI 87
           PI  +H    SSSSSPFTEKHS++RYQRD W+YK       +  S S   D   +R++DI
Sbjct: 26  PICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVRENDI 85

Query: 88  ALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADL 147
           A QLPELKKLL VL+EKRV  GC  GG+CGPG+V+LVGTGPGDPELLTLKAV+VIQSADL
Sbjct: 86  ASQLPELKKLLAVLKEKRVK-GC-KGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQSADL 145

Query: 148 LLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 207
           LLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP
Sbjct: 146 LLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 205

Query: 208 LVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGG 267
           LVFGRGGEEMDFLQQ+GI+V+++PGITAASGIAAELGIPLTHRGVAT+VRFLTGHSRKGG
Sbjct: 206 LVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRKGG 265

Query: 268 TDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFA 327
           TDPLFVAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRTVFA
Sbjct: 266 TDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRTVFA 325

Query: 328 ELKDLADEIKAAELVSPTLIIIGKVVSLSPHWSLSSKEASCLVE 369
           ELKD A EI++A LVSPTLIIIGKVV LSP W   +KE+SCLVE
Sbjct: 326 ELKDFATEIQSAGLVSPTLIIIGKVVELSPLWPHCTKESSCLVE 367

[Close]

BLAST of MS009330 vs. TAIR 10
Match: AT5G40850.2 (urophorphyrin methylase 1 )

HSP 1 Score: 466.8 bits (1200), Expect = 1.6e-131
Identity = 244/314 (77.71%), Postives = 267/314 (85.03%), Query Frame = 0


Query: 28  PISSIH---CSSSSSPFTEKHSIKRYQRDDWVYKNQADQASTALSCSVPCDSDSIRQDDI 87
           PI  +H    SSSSSPFTEKHS++RYQRD W+YK       +  S S   D   +R++DI
Sbjct: 26  PICCLHYNTASSSSSPFTEKHSVERYQRDQWLYKAVEPTPPSTPSPSPFEDEVFVRENDI 85

Query: 88  ALQLPELKKLLMVLREKRVNGGCDDGGECGPGNVFLVGTGPGDPELLTLKAVKVIQSADL 147
           A QLPELKKLL VL+EKRV  GC  GG+CGPG+V+LVGTGPGDPELLTLKAV+VIQSADL
Sbjct: 86  ASQLPELKKLLAVLKEKRVK-GC-KGGDCGPGDVYLVGTGPGDPELLTLKAVRVIQSADL 145

Query: 148 LLYDRLVSNDVLDLVGPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 207
           LLYDRLVSNDVL+LV PDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP
Sbjct: 146 LLYDRLVSNDVLELVAPDARLLYVGKTAGYHSRTQEEIHELLLNFAEAGATVVRLKGGDP 205

Query: 208 LVFGRGGEEMDFLQQKGIQVKIVPGITAASGIAAELGIPLTHRGVATNVRFLTGHSRKGG 267
           LVFGRGGEEMDFLQQ+GI+V+++PGITAASGIAAELGIPLTHRGVAT+VRFLTGHSRKGG
Sbjct: 206 LVFGRGGEEMDFLQQQGIRVQVIPGITAASGIAAELGIPLTHRGVATSVRFLTGHSRKGG 265

Query: 268 TDPLFVAENAADPDSTLVVYMGLSTLPSLALKLMHHGLPPDTPAAAVERGTTPQQRTVFA 327
           TDPLFVAENAADPD+TLVVYMGL TLPSLA KLM HGLP DTPA AVERGTTP QRT F 
Sbjct: 266 TDPLFVAENAADPDTTLVVYMGLGTLPSLAQKLMDHGLPSDTPAVAVERGTTPLQRTAFG 325

Query: 328 ELKDLADEIKAAEL 339
            L      +K  +L
Sbjct: 326 ILCRFLLSLKILQL 337

[Close]

BLAST of MS009330 vs. TAIR 10
Match: AT1G45110.1 (Tetrapyrrole (Corrin/Porphyrin) Methylases )

HSP 1 Score: 45.4 bits (106), Expect = 1.1e-04
Identity = 44/152 (28.95%), Postives = 69/152 (45.39%), Query Frame = 0


Query: 111 GECGPGNVFLVGTGPGDPELLTLKAVKVIQSADLLL-YDRLVSNDVLDLVGPDARLLYVG 170
           G   PG ++LVGT  G+ E +TL+A++V++SAD++L  D   S  +L      A+LL   
Sbjct: 51  GPLKPG-LYLVGTPIGNLEDITLRAIRVLRSADVILSEDTRHSGKLLQYYNIKAQLL--- 110

Query: 171 KTAGYHSRTQEEIHELLLNFAEAGATVVRLK-GGDPLVFGRGGEEMDFLQQKGIQVKIVP 230
               YH   + +  + +L   + G  V  +   G P +   G +      ++ I V  +P
Sbjct: 111 ---SYHKFNEAQREQAVLTRLKQGEIVALISDAGTPGISDPGTQLAKMCAKENIDVIPIP 170

Query: 231 GITAASGIAAELGIPLTHRGVATNVRFLTGHS 261
           G   A  + A L          T V FL  HS
Sbjct: 171 G---ACAVVAALSASGLETDEFTFVGFLPKHS 192

The following BLAST results are available for this feature:

BLAST of MS009330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149015.1	3.4e-205	99.46	uncharacterized protein LOC111017535 [Momordica charantia]	[more]
XP_022991905.1	4.6e-186	91.33	uncharacterized protein LOC111488402 [Cucurbita maxima]	[more]
XP_023548332.1	3.9e-185	90.79	uncharacterized protein LOC111807000 [Cucurbita pepo subsp. pepo]	[more]
XP_008461254.1	4.3e-184	89.81	PREDICTED: uroporphyrinogen-III C-methyltransferase [Cucumis melo]	[more]
XP_004135909.1	1.1e-182	89.01	uncharacterized protein LOC101222061 [Cucumis sativus] >KGN45161.1 hypothetical ...	[more]

BLAST of MS009330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42606	2.3e-148	79.94	S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloro...	[more]
B8GUD3	3.7e-61	49.80	Siroheme synthase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 ...	[more]
Q6F8G6	8.2e-61	52.32	Siroheme synthase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=...	[more]
Q0VQ05	8.2e-61	50.21	Siroheme synthase OS=Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / N...	[more]
Q02NA3	2.4e-60	49.02	Siroheme synthase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) OX=208963 GN=cys...	[more]

BLAST of MS009330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4K3	1.7e-205	99.46	uncharacterized protein LOC111017535 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1JU84	2.2e-186	91.33	uncharacterized protein LOC111488402 OS=Cucurbita maxima OX=3661 GN=LOC111488402...	[more]
A0A1S3CDT8	2.1e-184	89.81	uroporphyrinogen-III C-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499894...	[more]
A0A0A0KBI8	5.2e-183	89.01	TP_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G42898...	[more]
A0A6J1GQM1	5.7e-182	88.83	uncharacterized protein LOC111456604 OS=Cucurbita moschata OX=3662 GN=LOC1114566...	[more]

BLAST of MS009330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G40850.1	1.7e-149	79.94	urophorphyrin methylase 1	[more]
AT5G40850.2	1.6e-131	77.71	urophorphyrin methylase 1	[more]
AT1G45110.1	1.1e-04	28.95	Tetrapyrrole (Corrin/Porphyrin) Methylases	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000878	Tetrapyrrole methylase	PFAM	PF00590	TP_methylase	coord: 118..330 e-value: 4.1E-50 score: 170.7
IPR014777	Tetrapyrrole methylase, subdomain 1	GENE3D	3.40.1010.10		coord: 109..231 e-value: 1.0E-41 score: 144.0
IPR014776	Tetrapyrrole methylase, subdomain 2	GENE3D	3.30.950.10		coord: 232..367 e-value: 3.6E-44 score: 152.0
IPR006366	Uroporphyrin-III C-methyltransferase	TIGRFAM	TIGR01469	TIGR01469	coord: 116..352 e-value: 6.0E-87 score: 289.3
IPR006366	Uroporphyrin-III C-methyltransferase	CDD	cd11642	SUMT	coord: 121..350 e-value: 5.17811E-124 score: 354.821
None	No IPR available	PANTHER	PTHR45790:SF3	UROPORPHYRINOGEN-III C-METHYLTRANSFERASE	coord: 112..368
None	No IPR available	PANTHER	PTHR45790	SIROHEME SYNTHASE-RELATED	coord: 112..368
IPR003043	Uroporphiryn-III C-methyltransferase, conserved site	PROSITE	PS00840	SUMT_2	coord: 196..229
IPR003043	Uroporphiryn-III C-methyltransferase, conserved site	PROSITE	PS00839	SUMT_1	coord: 121..135
IPR035996	Tetrapyrrole methylase superfamily	SUPERFAMILY	53790	Tetrapyrrole methylase	coord: 115..356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009330.1	MS009330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019354	siroheme biosynthetic process
molecular_function	GO:0008168	methyltransferase activity