MS009301 (gene) Bitter gourd (TR) v1

Overview


Name	MS009301
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	E3 ubiquitin ligase BIG BROTHER
Location	scaffold813: 168460 .. 171013 (-)
RNA-Seq Expression	MS009301
Synteny	MS009301

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGAGGACAGACAAATGGAAGTACATTATATCGACACCGGCTTCCCGTACACGACGACAGAGAGCTTTATGGATTTCTTTGAAGGGCTCCATCACCACCAGGTACCTGCTTATGGCAATGCTGGGCCATTGCTTGATCAGGTATTCCGAAAAGTCGAAACTTTTATGAAAAACGATGAAAATTTTCCGATACTTGAACTCAGGTCTTGTAAGTTGGTAGTAATTAGTATTGGAAATGTATGGTACTTTGGTTCTTAGGGAAATGCATACTGGTCGATGAATATGCAATGTTACAAATTTGGAGTATCCGATCCCGGGAATACATATTACGGCCATTCCGAGGAAAGTCATCATTTGCCACCTTCAATGGATATTGGTGAAAGGGGGTGGGAGTATCCTTCAACCGTGACTGTCGAAAATCCTGAACCGACGTATGCACCATCGGTGGAGGAGGATGTTGTAGATGCTCATTCTATACCCGAAGAAGAATGTAAGTGTTGGCAGATCTGATAATGATACCAAGTCTGTTCAAATGATAACGTTGATTTTTTTTTTTGCAAACTGTCGAGTTTAATTGTCGAAAATGTTTGAAGAACGGCATAGTTGCAGATCTTATCATCAGCTTTTTACTTTGTTTTCCTGTTTTGCAGGTGATTTCAGTCATCAAGAGGAGGAGACCAGTACATCTCAAGTATGATTCTTCGTCGTTGATTTTGCAATATGCCTCTTCTTTTATTTCCATACAAAAGTCACCAACAAGTTTTAGGATCATCATCTACCTAATTCCTCTTACGAATTGTGGTTTAATCGAAACGTTATTCGGGATCCTACATGCGTATCTGTTTATTAATAAATTGGGACATAACCTCGTTATCATCAAAGATTGTCAAATGTTTATATACTGTTGTACTAAAGTTATCAAGTGGCTGATTGAATTACTTTTGTCCTCAACTTTAACTTTTGTCGCTTTTACACTTGAGAATACACCTTTTTTAGTTCAAAACTCATCGAATCCATCTCATTTTGTTTGTCTCCTCAGGTTGTATGGCAAGATGAGATTGATCCTGATCACATGACCTATGAGGTAACGTTATCTTTTAGAAACACAATATGATAGAGATTCTCATTCCGTTTTCTAGACGAAACTTGTTTCTATGACGACTTGTAGGATTGTTCCATGAAGGAATGAAAAGATCATTTGGCATACGATTCTCATGCTTTAAGATGTTTCCCATCTTGAGTAATATATTTGCGATTAGTAATCACGACCACTGCGATGATTTCGAGAGAAAGAGGAAAAATTATGCTATGACAACATTATAACCTTAAAATCTCCATACCAAACCAGTTTTAGATCAAGGATCAATCATCTTTGTTTTTTGTTTTTGATCAAAATTATTGCTGACATAAGAAGCTTTTAGGCTCAATTGCTTTAACTTTTAACAGTTTGTGATAAAAAGTTTTTAGGCTCAATTTTCAAACAAGCCCCTAATTCCCTTTGTAAATTACCATTTGAACTATGTGTGGCTGTCAAATAAATGAGAACCAAAGGACTTGACTGTAAAAAGAAAAAGCTTCAATGGGAATGTCTGGAGCCATAACTGGACATTTTACTACACGATTTTACCAGGAACTATTGGACTTGGGCGAATCGGTTGGAACTCAAAGTCGGGGGCTTTCTGAAGAACAAATAAGTCTGCTTCCGACTGCGAAATGCAAGTTAACGAGCTTTTTCTCGAGAAAAAAGCCAGAGGAAAGGTATGGTTTTTGACATATGTTGCATTAGAGTAAACTCGCTCAATGTTTGGTTAATCTGCTTTACTTCGAGGGCATAGGAACATTCCTGTCAAAAGAACTTGTAATGATCTATTGGTAATCGGTCCCGAAACATTTTAAACAACAGTTTCTATGAATTAGGATACAATGTCGGTCTTTATATGATGTTTGATTCTGATACGTTTACCTTAGAGTCATATGCATCCATTTTTCCGTCCTAGTACGCGAGATAGTACTGTCAAATGAACCTATACTCTTTGATGTCCTTGGTGTGTTGTGTAGCCATGTCGAATCTCTAACATATGATACAAATATTCCACAAATCTCCAAGCTTAAATACCATGGTTAGAAAGCATTAAAAAACGCTAATAATTCGGTTTAGCAGTAGATTGTTCTCGGAGTTATTTCTTATGTTGCTGATTGTAGATTAGTACTTTCTCGGTACGGTATTTACATTCAGGATCTCGGTTTAATATTGATTGTACTTGTTTCGTTTCGACTGCAGATGTGTGATTTGCCAAATGAGGTACAAGAGAGGGGACAAACAAATAAAGTTGCCCTGCAAGCATTTATACCATAACAAATGCATAACCAAATGGCTTACTATAAACAAAGTCAGTTCCTTTCTTGACTTTCTTATATCTTCCTATTTCCACCATTTCTACTTCTAATTTCCTTTCATCTCACAAATCGTCATTGTCATTGTCAGATATGTCCGATCTGCAACATCGAGGTGTTTGGAGACGAAGGCATGAATGCGTCTGGCAGCGATAAC

mRNA sequence

ATGAATGAGGACAGACAAATGGAAGTACATTATATCGACACCGGCTTCCCGTACACGACGACAGAGAGCTTTATGGATTTCTTTGAAGGGCTCCATCACCACCAGGTACCTGCTTATGGCAATGCTGGGCCATTGCTTGATCAGGGAAATGCATACTGGTCGATGAATATGCAATGTTACAAATTTGGAGTATCCGATCCCGGGAATACATATTACGGCCATTCCGAGGAAAGTCATCATTTGCCACCTTCAATGGATATTGGTGAAAGGGGGTGGGAGTATCCTTCAACCGTGACTGTCGAAAATCCTGAACCGACGTATGCACCATCGGTGGAGGAGGATGTTGTAGATGCTCATTCTATACCCGAAGAAGAATGTAATTCAAAACTCATCGAATCCATCTCATTTTGTTTGTCTCCTCAGGTTGTATGGCAAGATGAGATTGATCCTGATCACATGACCTATGAGGAACTATTGGACTTGGGCGAATCGGTTGGAACTCAAAGTCGGGGGCTTTCTGAAGAACAAATAAGTCTGCTTCCGACTGCGAAATGCAAGTTAACGAGCTTTTTCTCGAGAAAAAAGCCAGAGGAAAGATGTGTGATTTGCCAAATGAGGTACAAGAGAGGGGACAAACAAATAAAGTTGCCCTGCAAGCATTTATACCATAACAAATGCATAACCAAATGGCTTACTATAAACAAAATATGTCCGATCTGCAACATCGAGGTGTTTGGAGACGAAGGCATGAATGCGTCTGGCAGCGATAAC

Coding sequence (CDS)

ATGAATGAGGACAGACAAATGGAAGTACATTATATCGACACCGGCTTCCCGTACACGACGACAGAGAGCTTTATGGATTTCTTTGAAGGGCTCCATCACCACCAGGTACCTGCTTATGGCAATGCTGGGCCATTGCTTGATCAGGGAAATGCATACTGGTCGATGAATATGCAATGTTACAAATTTGGAGTATCCGATCCCGGGAATACATATTACGGCCATTCCGAGGAAAGTCATCATTTGCCACCTTCAATGGATATTGGTGAAAGGGGGTGGGAGTATCCTTCAACCGTGACTGTCGAAAATCCTGAACCGACGTATGCACCATCGGTGGAGGAGGATGTTGTAGATGCTCATTCTATACCCGAAGAAGAATGTAATTCAAAACTCATCGAATCCATCTCATTTTGTTTGTCTCCTCAGGTTGTATGGCAAGATGAGATTGATCCTGATCACATGACCTATGAGGAACTATTGGACTTGGGCGAATCGGTTGGAACTCAAAGTCGGGGGCTTTCTGAAGAACAAATAAGTCTGCTTCCGACTGCGAAATGCAAGTTAACGAGCTTTTTCTCGAGAAAAAAGCCAGAGGAAAGATGTGTGATTTGCCAAATGAGGTACAAGAGAGGGGACAAACAAATAAAGTTGCCCTGCAAGCATTTATACCATAACAAATGCATAACCAAATGGCTTACTATAAACAAAATATGTCCGATCTGCAACATCGAGGTGTTTGGAGACGAAGGCATGAATGCGTCTGGCAGCGATAAC

Protein sequence

MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHSIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPICNIEVFGDEGMNASGSDN

Homology

[Close]

BLAST of MS009301 vs. NCBI nr
Match: XP_022148735.1 (E3 ubiquitin ligase BIG BROTHER [Momordica charantia])

HSP 1 Score: 510.0 bits (1312), Expect = 1.2e-140
Identity = 241/257 (93.77%), Postives = 245/257 (95.33%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHS 120
           KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAH 
Sbjct: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHF 120

Query: 121 IPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLL 180
           IPEEEC+    E  +   + QVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLL
Sbjct: 121 IPEEECDFSHQEEET--STSQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLL 180

Query: 181 PTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPIC 240
           PTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPIC
Sbjct: 181 PTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 240

Query: 241 NIEVFGDEGMNASGSDN 258
           NIEVFGDEG+NASGSDN
Sbjct: 241 NIEVFGDEGVNASGSDN 255

[Close]

BLAST of MS009301 vs. NCBI nr
Match: KAA0058634.1 (E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 469.2 bits (1206), Expect = 2.3e-128
Identity = 225/258 (87.21%), Postives = 235/258 (91.09%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAH 120
           KFGVSD GNTYYGHSEESHHLPPSMD+GER WEYP S V VE PEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISL 180
           SIP EEC   LI S+ FC S QV+WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISL
Sbjct: 121 SIP-EEC-KWLIVSVFFCSSLQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISL 180

Query: 181 LPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPI 240
           LPTA+CKLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPI
Sbjct: 181 LPTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPI 240

Query: 241 CNIEVFGDEGMNASGSDN 258
           CNIEVFGD   NAS SDN
Sbjct: 241 CNIEVFGDGSTNASASDN 256

[Close]

BLAST of MS009301 vs. NCBI nr
Match: XP_038899262.1 (E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899263.1 E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899264.1 E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida])

HSP 1 Score: 467.6 bits (1202), Expect = 6.8e-128
Identity = 226/258 (87.60%), Postives = 234/258 (90.70%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQMEVHYIDTGFPYT TESFMDFFEGLH HQVPAYGNA PLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTV-TVENPEPTYAPSVEEDVVDAH 120
           KFGVSD GNTYYGHSEESHHLPPSMD+GER WEYP++V  VE PEPTYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAH 120

Query: 121 SIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISL 180
           SIP EEC+    E  S   + QV WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISL
Sbjct: 121 SIP-EECDLSHQEETS---TSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISL 180

Query: 181 LPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPI 240
           LPTA+ KLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPI
Sbjct: 181 LPTARFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPI 240

Query: 241 CNIEVFGDEGMNASGSDN 258
           CNIEVFGD  MNASG+DN
Sbjct: 241 CNIEVFGDGSMNASGNDN 254

[Close]

BLAST of MS009301 vs. NCBI nr
Match: TYK10442.1 (E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 460.3 bits (1183), Expect = 1.1e-125
Identity = 221/258 (85.66%), Postives = 232/258 (89.92%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAH 120
           KFGVSD GNTYYGHSEESHHLPPSMD+GER WEYP S V VE PEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISL 180
           SIP EEC+    E  S   + QV+WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISL
Sbjct: 121 SIP-EECDLSHREETS---TSQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISL 180

Query: 181 LPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPI 240
           LPTA+CKLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPI
Sbjct: 181 LPTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPI 240

Query: 241 CNIEVFGDEGMNASGSDN 258
           CNIEVFGD   NAS SDN
Sbjct: 241 CNIEVFGDGSTNASASDN 254

[Close]

BLAST of MS009301 vs. NCBI nr
Match: XP_008461282.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo] >XP_008461283.1 PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo])

HSP 1 Score: 459.1 bits (1180), Expect = 2.4e-125
Identity = 221/258 (85.66%), Postives = 231/258 (89.53%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAH 120
           KFGVSD GNTYYGHSEESHHLPPSMD+GER WEYP S V VE PEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISL 180
           SIP EEC+    E  S   + QV WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISL
Sbjct: 121 SIP-EECDLSHREETS---TSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISL 180

Query: 181 LPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPI 240
           LPTA+CKLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPI
Sbjct: 181 LPTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPI 240

Query: 241 CNIEVFGDEGMNASGSDN 258
           CNIEVFGD   NAS SDN
Sbjct: 241 CNIEVFGDGSTNASASDN 254

[Close]

BLAST of MS009301 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.0e-62
Identity = 128/255 (50.20%), Postives = 168/255 (65.88%), Query Frame = 0


Query: 1   MNEDRQ--MEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G     +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDPGN-TYYGHSEESHHLPPSMDIGERGWEYPSTVTV-ENPEPTYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M IG   W+Y   V V ++PE T A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQ 180
           D HS   EEC    I +     SPQV WQD+IDPD MTYEEL++LGE+VGT+SRGLS+E 
Sbjct: 121 DEHS-EAEEC----IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQEL 180

Query: 181 ISLLPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKI 240
           I  LPT K K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH+YH++CI+KWL+INK+
Sbjct: 181 IETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKV 240

Query: 241 CPICNIEVFGDEGMN 252
           CP+CN EVFG+  ++
Sbjct: 241 CPVCNSEVFGEPSIH 248

[Close]

BLAST of MS009301 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.6e-23
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 0


Query: 141 QVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERC 200
           Q  W DE+DPD ++YEELL LG+ VGT+SRGLS + I+ LP+ + K     ++    E C
Sbjct: 232 QDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD--NQNGTNESC 291

Query: 201 VICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPICNIEV 245
           VIC++ Y+  +  I LPCKH YH++CI  WL INK+CP+C+ EV
Sbjct: 292 VICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEV 332

[Close]

BLAST of MS009301 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.0e-14
Identity = 38/93 (40.86%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 148 IDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERCVICQMRY 207
           +D D+M+YEELL LGE +G  S GLSEE I  +       +S     +  E C +CQ  Y
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEY 626

Query: 208 KRGDKQIKLPCKHLYHNKCITKWLTINKICPIC 241
             GD    L C H +H  C+ +WL +  +CPIC
Sbjct: 627 AEGDDLGTLGCGHEFHTACVKQWLMLKNLCPIC 659

[Close]

BLAST of MS009301 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.4e-13
Identity = 44/101 (43.56%), Postives = 53/101 (52.48%), Query Frame = 0


Query: 148 IDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPE-----ERCVI 207
           +D D+M+YEELL LGE +G  S GLSEE I  L   K    +  S    E     E C I
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI--LKAMKQHKHTSSSPSSVELHQNIEPCCI 660

Query: 208 CQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPICNIE 244
           CQ  Y  GD    L C H +H  CI +W+ I  +CPIC  E
Sbjct: 661 CQEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of MS009301 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 7.6e-13
Identity = 38/93 (40.86%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 148 IDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERCVICQMRY 207
           +D D+M+YEELL L E +G  S GLSEE+++ L   +   +        EE C ICQ  Y
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEY 627

Query: 208 KRGDKQIKLPCKHLYHNKCITKWLTINKICPIC 241
             GD    L C H +H  C+ +WL +   CPIC
Sbjct: 628 VDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]

BLAST of MS009301 vs. ExPASy TrEMBL
Match: A0A6J1D3R4 (E3 ubiquitin ligase BIG BROTHER OS=Momordica charantia OX=3673 GN=LOC111017324 PE=4 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 5.8e-141
Identity = 241/257 (93.77%), Postives = 245/257 (95.33%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHS 120
           KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAH 
Sbjct: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYPSTVTVENPEPTYAPSVEEDVVDAHF 120

Query: 121 IPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLL 180
           IPEEEC+    E  +   + QVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLL
Sbjct: 121 IPEEECDFSHQEEET--STSQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLL 180

Query: 181 PTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPIC 240
           PTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPIC
Sbjct: 181 PTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 240

Query: 241 NIEVFGDEGMNASGSDN 258
           NIEVFGDEG+NASGSDN
Sbjct: 241 NIEVFGDEGVNASGSDN 255

[Close]

BLAST of MS009301 vs. ExPASy TrEMBL
Match: A0A5A7UTY6 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001030 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.1e-128
Identity = 225/258 (87.21%), Postives = 235/258 (91.09%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAH 120
           KFGVSD GNTYYGHSEESHHLPPSMD+GER WEYP S V VE PEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISL 180
           SIP EEC   LI S+ FC S QV+WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISL
Sbjct: 121 SIP-EEC-KWLIVSVFFCSSLQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISL 180

Query: 181 LPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPI 240
           LPTA+CKLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPI
Sbjct: 181 LPTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPI 240

Query: 241 CNIEVFGDEGMNASGSDN 258
           CNIEVFGD   NAS SDN
Sbjct: 241 CNIEVFGDGSTNASASDN 256

[Close]

BLAST of MS009301 vs. ExPASy TrEMBL
Match: A0A5D3CGQ8 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00890 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.3e-126
Identity = 221/258 (85.66%), Postives = 232/258 (89.92%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAH 120
           KFGVSD GNTYYGHSEESHHLPPSMD+GER WEYP S V VE PEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISL 180
           SIP EEC+    E  S   + QV+WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISL
Sbjct: 121 SIP-EECDLSHREETS---TSQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISL 180

Query: 181 LPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPI 240
           LPTA+CKLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPI
Sbjct: 181 LPTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPI 240

Query: 241 CNIEVFGDEGMNASGSDN 258
           CNIEVFGD   NAS SDN
Sbjct: 241 CNIEVFGDGSTNASASDN 254

[Close]

BLAST of MS009301 vs. ExPASy TrEMBL
Match: A0A1S3CED1 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.2e-125
Identity = 221/258 (85.66%), Postives = 231/258 (89.53%), Query Frame = 0


Query: 1   MNEDRQMEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDPGNTYYGHSEESHHLPPSMDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAH 120
           KFGVSD GNTYYGHSEESHHLPPSMD+GER WEYP S V VE PEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISL 180
           SIP EEC+    E  S   + QV WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISL
Sbjct: 121 SIP-EECDLSHREETS---TSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISL 180

Query: 181 LPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPI 240
           LPTA+CKLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPI
Sbjct: 181 LPTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPI 240

Query: 241 CNIEVFGDEGMNASGSDN 258
           CNIEVFGD   NAS SDN
Sbjct: 241 CNIEVFGDGSTNASASDN 254

[Close]

BLAST of MS009301 vs. ExPASy TrEMBL
Match: A0A1S3CES7 (E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 6.0e-122
Identity = 215/252 (85.32%), Postives = 225/252 (89.29%), Query Frame = 0


Query: 7   MEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQCYKFGVSD 66
           ME+HY+DTGFPYT TESFMDFF+GLH HQVPAYGNA PLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  PGNTYYGHSEESHHLPPSMDIGERGWEYP-STVTVENPEPTYAPSVEEDVVDAHSIPEEE 126
            GNTYYGHSEESHHLPPSMD+GER WEYP S V VE PEP+YAPSVEEDVVDAHSIP EE
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIP-EE 120

Query: 127 CNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKC 186
           C+    E  S   + QV WQDEIDPDHMTYEELLDLGESVGT+SRGLSEEQISLLPTA+C
Sbjct: 121 CDLSHREETS---TSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARC 180

Query: 187 KLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPICNIEVF 246
           KLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKH YHNKCITKWLTINKICPICNIEVF
Sbjct: 181 KLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVF 240

Query: 247 GDEGMNASGSDN 258
           GD   NAS SDN
Sbjct: 241 GDGSTNASASDN 248

[Close]

BLAST of MS009301 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 241.5 bits (615), Expect = 7.4e-64
Identity = 128/255 (50.20%), Postives = 168/255 (65.88%), Query Frame = 0


Query: 1   MNEDRQ--MEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G     +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDPGN-TYYGHSEESHHLPPSMDIGERGWEYPSTVTV-ENPEPTYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M IG   W+Y   V V ++PE T A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQ 180
           D HS   EEC    I +     SPQV WQD+IDPD MTYEEL++LGE+VGT+SRGLS+E 
Sbjct: 121 DEHS-EAEEC----IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQEL 180

Query: 181 ISLLPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKI 240
           I  LPT K K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH+YH++CI+KWL+INK+
Sbjct: 181 IETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKV 240

Query: 241 CPICNIEVFGDEGMN 252
           CP+CN EVFG+  ++
Sbjct: 241 CPVCNSEVFGEPSIH 248

[Close]

BLAST of MS009301 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 241.5 bits (615), Expect = 7.4e-64
Identity = 128/255 (50.20%), Postives = 168/255 (65.88%), Query Frame = 0


Query: 1   MNEDRQ--MEVHYIDTGFPYTTTESFMDFFEGLHHHQVPAYGNAGPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G     +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDPGN-TYYGHSEESHHLPPSMDIGERGWEYPSTVTV-ENPEPTYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M IG   W+Y   V V ++PE T A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQ 180
           D HS   EEC    I +     SPQV WQD+IDPD MTYEEL++LGE+VGT+SRGLS+E 
Sbjct: 121 DEHS-EAEEC----IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQEL 180

Query: 181 ISLLPTAKCKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKI 240
           I  LPT K K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH+YH++CI+KWL+INK+
Sbjct: 181 IETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKV 240

Query: 241 CPICNIEVFGDEGMN 252
           CP+CN EVFG+  ++
Sbjct: 241 CPVCNSEVFGEPSIH 248

[Close]

BLAST of MS009301 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 110.2 bits (274), Expect = 2.6e-24
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 0


Query: 141 QVVWQDEIDPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAKCKLTSFFSRKKPEERC 200
           Q  W DE+DPD ++YEELL LG+ VGT+SRGLS + I+ LP+ + K     ++    E C
Sbjct: 232 QDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD--NQNGTNESC 291

Query: 201 VICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPICNIEV 245
           VIC++ Y+  +  I LPCKH YH++CI  WL INK+CP+C+ EV
Sbjct: 292 VICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEV 332

[Close]

BLAST of MS009301 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 102.8 bits (255), Expect = 4.1e-22
Identity = 52/105 (49.52%), Postives = 70/105 (66.67%), Query Frame = 0


Query: 149 DPDHMTYEELLDLGESVGTQSRGLSEEQISLLPTAK--CKLTSFF---SRKK---PEERC 208
           DP++MTYEEL +LG+SVG   +GLS+E+IS L T K   K  S++    +KK    + +C
Sbjct: 175 DPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYCLHMKKKFVADDSQC 234

Query: 209 VICQMRYKRGDKQIKLPCKHLYHNKCITKWLTINKICPICNIEVF 246
            IC M Y +GDK   LPCKH+YH  CI++WL  NK+C IC  EV+
Sbjct: 235 SICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]

BLAST of MS009301 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 5.4e-22
Identity = 57/132 (43.18%), Postives = 82/132 (62.12%), Query Frame = 0


Query: 111 VEEDVVDAHSIPEEECNSKLIESISFCLSPQVVWQDEIDPDHMTYEELLDLGESVGTQSR 170
           V+ED ++   + ++E NS L E   F     +  +DEIDPD ++YEEL+ LG+ +G ++R
Sbjct: 81  VDEDEIN-EFLEDQESNSNLEEEDDF-----LEEEDEIDPDQLSYEELIALGDFIGVENR 140

Query: 171 GLSEEQISLLPTAKCKLTSFFSRKKPE-ERCVICQMRYKRGDKQIKL-PCKHLYHNKCIT 230
           GL+  +IS    A    T  FS  K E +RCV+CQM ++  +  + L PC H YH++CIT
Sbjct: 141 GLTPIEISTCLNAS---TYVFSHNKNEIDRCVVCQMEFEERESLVVLRPCDHPYHSECIT 200

Query: 231 KWLTINKICPIC 241
           KWL   KICPIC
Sbjct: 201 KWLETKKICPIC 203

The following BLAST results are available for this feature:

BLAST of MS009301 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148735.1	1.2e-140	93.77	E3 ubiquitin ligase BIG BROTHER [Momordica charantia]	[more]
KAA0058634.1	2.3e-128	87.21	E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa]	[more]
XP_038899262.1	6.8e-128	87.60	E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899263.1 E3 u...	[more]
TYK10442.1	1.1e-125	85.66	E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008461282.1	2.4e-125	85.66	PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo] >XP_0084612...	[more]

BLAST of MS009301 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	1.0e-62	50.20	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	3.6e-23	50.00	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
O49500	9.0e-14	40.86	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q9ZQF9	3.4e-13	43.56	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
Q7XTV7	7.6e-13	40.86	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of MS009301 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3R4	5.8e-141	93.77	E3 ubiquitin ligase BIG BROTHER OS=Momordica charantia OX=3673 GN=LOC111017324 P...	[more]
A0A5A7UTY6	1.1e-128	87.21	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A5D3CGQ8	5.3e-126	85.66	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3CED1	1.2e-125	85.66	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A1S3CES7	6.0e-122	85.32	E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]

BLAST of MS009301 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	7.4e-64	50.20	RING/U-box superfamily protein	[more]
AT3G63530.2	7.4e-64	50.20	RING/U-box superfamily protein	[more]
AT3G19910.1	2.6e-24	50.00	RING/U-box superfamily protein	[more]
AT5G52140.1	4.1e-22	49.52	RING/U-box superfamily protein	[more]
AT3G47180.1	5.4e-22	43.18	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 200..240 e-value: 1.5E-4 score: 31.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 199..241 e-value: 2.7E-11 score: 43.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 200..241 score: 11.559839
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 161..245 e-value: 4.2E-18 score: 66.8
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..249
None	No IPR available	PANTHER	PTHR46400:SF3	E3 UBIQUITIN-PROTEIN LIGASE BIG BROTHER	coord: 1..249
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 193..246

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009301.1	MS009301.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity