MS009102 (gene) Bitter gourd (TR) v1

Overview


Name	MS009102
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	mitogen-activated protein kinase kinase kinase 18-like
Location	scaffold687: 691447 .. 692481 (-)
RNA-Seq Expression	MS009102
Synteny	MS009102

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGAATGGGTTCGAGGAGAAGAACTGGGCCGCGGCAATTTCGCGACGATCAATTTAGCAATACCCACAAAGGGGTTCGATCAGTTTCCCCCATTAATGGCGGTCAAGTCCTCTGTAGCTGCATCCTCTGTTTCCGTTTCGCTGAAGAACGAGAAGCAAATTTTGGACCGAATTGGGAATTGCCCACAAATCGTTTCTTGTTTTGGCGATGGATTCACCATTGAAAGAGATGGGGAGAAGTATTATAATGTGTTGTTGGAGTACGCCAATGGCGGAAGTCTTGCTGATTCTGTCAAAATCCACGGCGGCGGGCTGCCGGAATTCGATGTTAGAAGATACACGGCGGCGATTCTTCGGGGGCTCCAGCATGTTCACGGCAATGGGTTTGTTCACTGCGATTTGAAGCTTGCGAATGTGTTGAATTTTGGCAATGGCGATGTGAAGATTGCTGATTTTGGGCTTGCCAAAGTGGCCGGAAAGAAGACGGCAGCGGAAACAGAGCAGAGGTTTGAATGGAGAGGGACTCCGATGTATATGTCGCCGGAGACTGTGACCGGCGGCGAGTATGAACCGCCGTGTGATATTTGGGCGCTGGGTTGCGCGGTCGTGGAGATGGTGGCCGGGAAACCGGCGTGGAATGTTCGGCCGGAAACCGACATTTTCGGGCTGATGATGAGAATCGGAGTTGGAGACGAAGTTCCGGAACTGCCGGAAAATTTGTCCGACGAGGGGAGAGATTTTCTCCGGCGGTGTTTCGTAAAGGATCCGAGGAAGAGATGGACGGCGGAGATGCTTCTGAATCACCCATTTGTCGCCGGCATTACTGTTACATTAAAGGAGGCCGAACCACCGATGGAGTCGCCGACCGGTCCTTTCGATTTCCCGGAATCGTTGCCGACGTGTTCCGACCAACCTTCCGATGATTCCTATTTTTGTTCTTCTACTGTCTCGCCGGAGACGATGAGTAGGCTGCGGCGGCTGGTGACCGAGAAGCCTTTGGATTGGTCTGTTTCGGATAGTTGGGTCACAGTTAGG

mRNA sequence

TTGGAATGGGTTCGAGGAGAAGAACTGGGCCGCGGCAATTTCGCGACGATCAATTTAGCAATACCCACAAAGGGGTTCGATCAGTTTCCCCCATTAATGGCGGTCAAGTCCTCTGTAGCTGCATCCTCTGTTTCCGTTTCGCTGAAGAACGAGAAGCAAATTTTGGACCGAATTGGGAATTGCCCACAAATCGTTTCTTGTTTTGGCGATGGATTCACCATTGAAAGAGATGGGGAGAAGTATTATAATGTGTTGTTGGAGTACGCCAATGGCGGAAGTCTTGCTGATTCTGTCAAAATCCACGGCGGCGGGCTGCCGGAATTCGATGTTAGAAGATACACGGCGGCGATTCTTCGGGGGCTCCAGCATGTTCACGGCAATGGGTTTGTTCACTGCGATTTGAAGCTTGCGAATGTGTTGAATTTTGGCAATGGCGATGTGAAGATTGCTGATTTTGGGCTTGCCAAAGTGGCCGGAAAGAAGACGGCAGCGGAAACAGAGCAGAGGTTTGAATGGAGAGGGACTCCGATGTATATGTCGCCGGAGACTGTGACCGGCGGCGAGTATGAACCGCCGTGTGATATTTGGGCGCTGGGTTGCGCGGTCGTGGAGATGGTGGCCGGGAAACCGGCGTGGAATGTTCGGCCGGAAACCGACATTTTCGGGCTGATGATGAGAATCGGAGTTGGAGACGAAGTTCCGGAACTGCCGGAAAATTTGTCCGACGAGGGGAGAGATTTTCTCCGGCGGTGTTTCGTAAAGGATCCGAGGAAGAGATGGACGGCGGAGATGCTTCTGAATCACCCATTTGTCGCCGGCATTACTGTTACATTAAAGGAGGCCGAACCACCGATGGAGTCGCCGACCGGTCCTTTCGATTTCCCGGAATCGTTGCCGACGTGTTCCGACCAACCTTCCGATGATTCCTATTTTTGTTCTTCTACTGTCTCGCCGGAGACGATGAGTAGGCTGCGGCGGCTGGTGACCGAGAAGCCTTTGGATTGGTCTGTTTCGGATAGTTGGGTCACAGTTAGG

Coding sequence (CDS)

TTGGAATGGGTTCGAGGAGAAGAACTGGGCCGCGGCAATTTCGCGACGATCAATTTAGCAATACCCACAAAGGGGTTCGATCAGTTTCCCCCATTAATGGCGGTCAAGTCCTCTGTAGCTGCATCCTCTGTTTCCGTTTCGCTGAAGAACGAGAAGCAAATTTTGGACCGAATTGGGAATTGCCCACAAATCGTTTCTTGTTTTGGCGATGGATTCACCATTGAAAGAGATGGGGAGAAGTATTATAATGTGTTGTTGGAGTACGCCAATGGCGGAAGTCTTGCTGATTCTGTCAAAATCCACGGCGGCGGGCTGCCGGAATTCGATGTTAGAAGATACACGGCGGCGATTCTTCGGGGGCTCCAGCATGTTCACGGCAATGGGTTTGTTCACTGCGATTTGAAGCTTGCGAATGTGTTGAATTTTGGCAATGGCGATGTGAAGATTGCTGATTTTGGGCTTGCCAAAGTGGCCGGAAAGAAGACGGCAGCGGAAACAGAGCAGAGGTTTGAATGGAGAGGGACTCCGATGTATATGTCGCCGGAGACTGTGACCGGCGGCGAGTATGAACCGCCGTGTGATATTTGGGCGCTGGGTTGCGCGGTCGTGGAGATGGTGGCCGGGAAACCGGCGTGGAATGTTCGGCCGGAAACCGACATTTTCGGGCTGATGATGAGAATCGGAGTTGGAGACGAAGTTCCGGAACTGCCGGAAAATTTGTCCGACGAGGGGAGAGATTTTCTCCGGCGGTGTTTCGTAAAGGATCCGAGGAAGAGATGGACGGCGGAGATGCTTCTGAATCACCCATTTGTCGCCGGCATTACTGTTACATTAAAGGAGGCCGAACCACCGATGGAGTCGCCGACCGGTCCTTTCGATTTCCCGGAATCGTTGCCGACGTGTTCCGACCAACCTTCCGATGATTCCTATTTTTGTTCTTCTACTGTCTCGCCGGAGACGATGAGTAGGCTGCGGCGGCTGGTGACCGAGAAGCCTTTGGATTGGTCTGTTTCGGATAGTTGGGTCACAGTTAGG

Protein sequence

LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGNCPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRGLQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMSPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPMESPTGPFDFPESLPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR

Homology

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BLAST of MS009102 vs. NCBI nr
Match: XP_022149480.1 (mitogen-activated protein kinase kinase kinase 18-like [Momordica charantia])

HSP 1 Score: 716.8 bits (1849), Expect = 8.7e-203
Identity = 343/345 (99.42%), Postives = 344/345 (99.71%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN
Sbjct: 3   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 62

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG
Sbjct: 63  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 122

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L+HVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPM MS
Sbjct: 123 LRHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMSMS 182

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL
Sbjct: 183 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 242

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPMESPTGPFDFPESLPT 300
           SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPMESPTGPFDFPESLPT
Sbjct: 243 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPMESPTGPFDFPESLPT 302

Query: 301 CSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
           CSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR
Sbjct: 303 CSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 347

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BLAST of MS009102 vs. NCBI nr
Match: KAG6605205.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035183.1 Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-160
Identity = 273/347 (78.67%), Postives = 305/347 (87.90%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRG+ELGRGNF TI+LAI TKGFDQFPPLMAVKSSV+ S  SVSLKNEK++LDRIGN
Sbjct: 1   MEWVRGDELGRGNFGTISLAILTKGFDQFPPLMAVKSSVSVSVSSVSLKNEKKVLDRIGN 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQIV+C+GDGF++ERDGEKYYN+ LEYANGGSLAD+VK+HGGGL EFDVRRYT AIL G
Sbjct: 61  CPQIVTCYGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L+HVHGNGFVHCDLKL+NVL FGNG+VKIADFGLAK A      ETE++FEWRGTPMYMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAPAETEETEEKFEWRGTPMYMS 180

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PETV GG+Y PPCD+WALGCAVVEMV GKPAW V+PETDIFGLMM IGVGDEVPELPENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVGGKPAWRVKPETDIFGLMMMIGVGDEVPELPENL 240

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGI--TVTLKEAEPPMESPTGPFDFPESL 300
           S+EG+DFLRRCFVKDPRKRWTAEMLLNHPFVAG   TVTLKE+    ESPTGPFDFPE +
Sbjct: 241 SEEGKDFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLTTESPTGPFDFPEFI 300

Query: 301 PTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
              S+Q  D+   C S+ + E M+RLRRLVTEKPLDWSV++SWVTVR
Sbjct: 301 -CSSNQRCDEMNLCCSSSTAEMMNRLRRLVTEKPLDWSVTESWVTVR 346

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BLAST of MS009102 vs. NCBI nr
Match: XP_022948206.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata])

HSP 1 Score: 573.2 bits (1476), Expect = 1.5e-159
Identity = 271/347 (78.10%), Postives = 304/347 (87.61%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRG+ELGRGNF TI+LAI TKGFDQFPPLMAVKSSV+ S  SVSLKNEK++LDRIGN
Sbjct: 1   MEWVRGDELGRGNFGTISLAILTKGFDQFPPLMAVKSSVSVSVSSVSLKNEKKVLDRIGN 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQIV+CFGDGF++ERDGEKYYN+ LEYANGGSLAD+VK+HGGGL EFDVRRYT AIL G
Sbjct: 61  CPQIVTCFGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L+HVHGNGFVHCDLKL+NVL FGNG+VKIADFGLAK A      ETE++FEWRGTPMYMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAAAETEETEEKFEWRGTPMYMS 180

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PETV GG+Y PPCD+WALGCAVVEMV GKPAW V+PETDIFGLMM IGVGDEVPELPENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVGGKPAWRVKPETDIFGLMMMIGVGDEVPELPENL 240

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGI--TVTLKEAEPPMESPTGPFDFPESL 300
           S+EG+DFLRRCFVKDPRKRWTAEMLLNHPFVAG   TVTLKE+    ESPTGPFDFPE +
Sbjct: 241 SEEGKDFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLTTESPTGPFDFPEFI 300

Query: 301 PTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
              S+Q  ++   C S+ + E M+RLRR+VTEKPLDWS ++SWVTVR
Sbjct: 301 -CSSNQRCEEMSLCCSSSTAEMMNRLRRVVTEKPLDWSFTESWVTVR 346

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BLAST of MS009102 vs. NCBI nr
Match: XP_038902011.1 (mitogen-activated protein kinase kinase kinase 18-like isoform X1 [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 7.7e-159
Identity = 280/348 (80.46%), Postives = 306/348 (87.93%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRG+ELGRGNFATI+LA  TKGFDQFPPLM VKSSV++ S SVSLKNEKQILDRIG 
Sbjct: 1   MEWVRGDELGRGNFATISLAKLTKGFDQFPPLMVVKSSVSSLS-SVSLKNEKQILDRIGI 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIH-GGGLPEFDVRRYTAAILR 120
           CPQI++CFGDGF++E+D EK YN+ LEYANGGSLAD V+IH GGGLPEFDVRRYT AIL 
Sbjct: 61  CPQIITCFGDGFSVEKDEEKRYNLFLEYANGGSLADVVRIHGGGGLPEFDVRRYTKAILC 120

Query: 121 GLQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYM 180
           GL+HVHGNGFVHCDLKL+NVL FGNGDVKI DFGLAK AGK TAAETE+R EWRGTPMYM
Sbjct: 121 GLRHVHGNGFVHCDLKLSNVLVFGNGDVKIVDFGLAKAAGKITAAETEERLEWRGTPMYM 180

Query: 181 SPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPEN 240
           SPETV GGEYE PCDIWALGCAVVEMVAGKPAW V PET+IFGLMMRIGVG+EVPE+PEN
Sbjct: 181 SPETVNGGEYESPCDIWALGCAVVEMVAGKPAWRVGPETNIFGLMMRIGVGEEVPEIPEN 240

Query: 241 LSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGI--TVTLKEAEPPMESPTGPFDFPES 300
           LS EG+DF+RRCFVKDPRKRWTAEMLLNHPFVAG   TVTLKEA    ESPTGPFDFPE 
Sbjct: 241 LSAEGKDFIRRCFVKDPRKRWTAEMLLNHPFVAGAGDTVTLKEA---TESPTGPFDFPEF 300

Query: 301 LPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
           +  CSDQ SD+  FC S  SPE +SR+RRLVT +P+DWSV DSWVTVR
Sbjct: 301 V--CSDQGSDEWSFCCSNSSPEMLSRVRRLVTGEPVDWSVMDSWVTVR 342

[Close]

BLAST of MS009102 vs. NCBI nr
Match: XP_023007353.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima])

HSP 1 Score: 569.7 bits (1467), Expect = 1.7e-158
Identity = 270/347 (77.81%), Postives = 302/347 (87.03%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRG+ELGRGNF TI+LA  TKGFDQFPPLMAVKSSV+ S  S SLKNEK++LDRIGN
Sbjct: 1   MEWVRGDELGRGNFGTISLARLTKGFDQFPPLMAVKSSVSVSVSSASLKNEKKVLDRIGN 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQIV+C+GDGF++ERDGEKYYN+ LEYANGGSLAD+VK+HGGGL EFDVRRYT AIL G
Sbjct: 61  CPQIVTCYGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L+HVHGNGFVHCDLKL+NVL FGNG+VKIADFGLAK A      ETE+ FEWRGTPMYMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAAAETEETEENFEWRGTPMYMS 180

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PETV GG+Y PPCD+WALGCAVVEMV GKPAW V+PETDIFGLMM IG+GDEVPELPENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVRGKPAWRVKPETDIFGLMMMIGIGDEVPELPENL 240

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGI--TVTLKEAEPPMESPTGPFDFPESL 300
           S+EG++FLRRCFVKDPRKRWTAEMLLNHPFVAG   TVTLKE+    ESPTGPFDFPE +
Sbjct: 241 SEEGKNFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLSTESPTGPFDFPEFV 300

Query: 301 PTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
              S+Q  D+   C S+ S E M+RLRRLVTEKPLDWSV++SWVTVR
Sbjct: 301 -CSSNQRCDEMNLCCSSSSAEMMNRLRRLVTEKPLDWSVTESWVTVR 346

[Close]

BLAST of MS009102 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.2e-92
Identity = 178/357 (49.86%), Postives = 237/357 (66.39%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRGE +G G F+T++ A  ++    FP L+AVKS+ A  + S+S  NEK +LD +G+
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLS--NEKSVLDSLGD 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGG-GLPEFDVRRYTAAILR 120
           CP+I+ C+G+  T+E +GE+ +N+LLEYA+ GSLA  +K  GG GLPE  VRR+T ++LR
Sbjct: 61  CPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLR 120

Query: 121 GLQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLA-KVAGKKTAAETEQRFEWRGTPMY 180
           GL+H+H  GF HCD+KLAN+L F +G VKIADFGLA +V G  TA    +  E RGTP+Y
Sbjct: 121 GLRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTA--LRKSVEIRGTPLY 180

Query: 181 MSPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPE 240
           M+PE V   EY    D+WALGCAVVEM +GK AW+V+  +    L++RIGVGDE+P++PE
Sbjct: 181 MAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPE 240

Query: 241 NLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVA---------GITVTLKEAEPPMESPT 300
            LS+EG+DFL +CFVKDP KRWTAEMLLNH FV             V +K+ +  + SP 
Sbjct: 241 MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPK 300

Query: 301 GPFDFPE-SLPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
            PF+F +    T    PS DS           + RL  LV+    DWSV  SW+TVR
Sbjct: 301 CPFEFDDWDSFTLDSNPSFDS----------PVERLGSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of MS009102 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.0e-48
Identity = 114/302 (37.75%), Postives = 161/302 (53.31%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EW RG  LGRG+ AT+  A      +    ++AVKSS      S  L+ E +IL  + +
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDE----ILAVKSSEVHR--SEFLQREAKILSSLSS 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
            P ++   G     E +G   YN+L+EYA  G+L D+    GG + E  V +YT  IL+G
Sbjct: 61  -PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKG 120

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFE--WRGTPMY 180
           L+++H  G VHCD+K +NV+    G+ KIADFG AK          +  FE    GTP +
Sbjct: 121 LEYIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAK--------RVDPVFESPVMGTPAF 180

Query: 181 MSPETVTGGEYEPPCDIWALGCAVVEMVAGKPAW-NVRPETDIFGLMMRIGVGDEVPELP 240
           M+PE   G +     DIWA+GC ++EMV G P W       D   ++ R+G   E PELP
Sbjct: 181 MAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELP 240

Query: 241 ENLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPM------ESPTGP 294
             L++E +DFL +C  ++  +RWTA  LLNHPF+     T  + EP +       SPT  
Sbjct: 241 CLLAEEAKDFLEKCLKREANERWTATQLLNHPFL----TTKPDIEPVLVPGLISNSPTSV 283

[Close]

BLAST of MS009102 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.8e-47
Identity = 130/358 (36.31%), Postives = 182/358 (50.84%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           + W RG+ LGRG+ AT++ A   +  +     +AVKS  A    S  L+ E +IL  + N
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGE----TLAVKS--AEFHRSEFLQREAKILSSL-N 60

Query: 61  CPQIVSCFGDGFTIE---RDGE-KYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAA 120
            P ++   G   T E    +GE   Y++L+EYA  G+L D    +GG + E  V +YT  
Sbjct: 61  SPYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQ 120

Query: 121 ILRGLQHVHGN-GFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGT 180
           IL GL+++H + G  HCD+K +NVL   NG+ KIADFG AK        E E     RGT
Sbjct: 121 ILLGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAK------WVEPEITEPVRGT 180

Query: 181 PMYMSPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPE 240
           P +M+PE   G       DIWA+GC V+EMV G   W     TD   ++ R+G   E+PE
Sbjct: 181 PAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPE 240

Query: 241 LPENLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPM------ESPT 300
           LP +L+++ +DFL +C  K+  +RWTA  LLNHPF       L   EP +       SPT
Sbjct: 241 LPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPF-------LVNKEPELVTGLVTNSPT 300

Query: 301 GPFD--FPESLPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
              D  F  S+     +     + C        +S +  +V E   D    D W+TVR
Sbjct: 301 SVTDQMFWRSVEEEVSEDRSSWWECHEDERIGVLSWIGHVVVESTWDLDGED-WITVR 337

[Close]

BLAST of MS009102 vs. ExPASy Swiss-Prot
Match: Q99759 (Mitogen-activated protein kinase kinase kinase 3 OS=Homo sapiens OX=9606 GN=MAP3K3 PE=1 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 7.0e-38
Identity = 101/278 (36.33%), Postives = 146/278 (52.52%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLA--------IPTKGFDQFPPLMAVKSSVAASSVSVSLKNEK 60
           + W RG+ LG+G F  + L         + +K   QF P      S   S    +L+ E 
Sbjct: 360 INWRRGKLLGQGAFGRVYLCYDVDTGRELASKQV-QFDP-----DSPETSKEVSALECEI 419

Query: 61  QILDRIGNCPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRR 120
           Q+L  + +  +IV  +G    +    EK   + +EY  GGS+ D +K + G L E   R+
Sbjct: 420 QLLKNLQH-ERIVQYYG---CLRDRAEKTLTIFMEYMPGGSVKDQLKAY-GALTESVTRK 479

Query: 121 YTAAILRGLQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEW 180
           YT  IL G+ ++H N  VH D+K AN+L    G+VK+ DFG +K       + T  R   
Sbjct: 480 YTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMR-SV 539

Query: 181 RGTPMYMSPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDE 240
            GTP +MSPE ++G  Y    D+W+LGC VVEM+  KP W    E +    + +I     
Sbjct: 540 TGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPW---AEYEAMAAIFKIATQPT 599

Query: 241 VPELPENLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPF 271
            P+LP ++S+ GRDFLRR FV + R+R +AE LL H F
Sbjct: 600 NPQLPSHISEHGRDFLRRIFV-EARQRPSAEELLTHHF 621

[Close]

BLAST of MS009102 vs. ExPASy Swiss-Prot
Match: Q61084 (Mitogen-activated protein kinase kinase kinase 3 OS=Mus musculus OX=10090 GN=Map3k3 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.2e-37
Identity = 101/278 (36.33%), Postives = 146/278 (52.52%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLA--------IPTKGFDQFPPLMAVKSSVAASSVSVSLKNEK 60
           + W RG+ LG+G F  + L         + +K   QF P      S   S    +L+ E 
Sbjct: 360 INWRRGKLLGQGAFGRVYLCYDVDTGRELASKQV-QFDP-----DSPETSKEVSALECEI 419

Query: 61  QILDRIGNCPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRR 120
           Q+L  + +  +IV  +G    +    EK   + +EY  GGS+ D +K + G L E   R+
Sbjct: 420 QLLKNLQH-ERIVQYYG---CLRDRAEKILTIFMEYMPGGSVKDQLKAY-GALTESVTRK 479

Query: 121 YTAAILRGLQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEW 180
           YT  IL G+ ++H N  VH D+K AN+L    G+VK+ DFG +K       + T  R   
Sbjct: 480 YTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGIR-SV 539

Query: 181 RGTPMYMSPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDE 240
            GTP +MSPE ++G  Y    D+W+LGC VVEM+  KP W    E +    + +I     
Sbjct: 540 TGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPW---AEYEAMAAIFKIATQPT 599

Query: 241 VPELPENLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPF 271
            P+LP ++S+ GRDFLRR FV + R+R +AE LL H F
Sbjct: 600 NPQLPSHISEHGRDFLRRIFV-EARQRPSAEELLTHHF 621

[Close]

BLAST of MS009102 vs. ExPASy TrEMBL
Match: A0A6J1D5U5 (mitogen-activated protein kinase kinase kinase 18-like OS=Momordica charantia OX=3673 GN=LOC111017899 PE=4 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 4.2e-203
Identity = 343/345 (99.42%), Postives = 344/345 (99.71%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN
Sbjct: 3   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 62

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG
Sbjct: 63  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 122

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L+HVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPM MS
Sbjct: 123 LRHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMSMS 182

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL
Sbjct: 183 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 242

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPMESPTGPFDFPESLPT 300
           SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPMESPTGPFDFPESLPT
Sbjct: 243 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPMESPTGPFDFPESLPT 302

Query: 301 CSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
           CSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR
Sbjct: 303 CSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 347

[Close]

BLAST of MS009102 vs. ExPASy TrEMBL
Match: A0A6J1G8M2 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111451851 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 7.5e-160
Identity = 271/347 (78.10%), Postives = 304/347 (87.61%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRG+ELGRGNF TI+LAI TKGFDQFPPLMAVKSSV+ S  SVSLKNEK++LDRIGN
Sbjct: 1   MEWVRGDELGRGNFGTISLAILTKGFDQFPPLMAVKSSVSVSVSSVSLKNEKKVLDRIGN 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQIV+CFGDGF++ERDGEKYYN+ LEYANGGSLAD+VK+HGGGL EFDVRRYT AIL G
Sbjct: 61  CPQIVTCFGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L+HVHGNGFVHCDLKL+NVL FGNG+VKIADFGLAK A      ETE++FEWRGTPMYMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAAAETEETEEKFEWRGTPMYMS 180

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PETV GG+Y PPCD+WALGCAVVEMV GKPAW V+PETDIFGLMM IGVGDEVPELPENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVGGKPAWRVKPETDIFGLMMMIGVGDEVPELPENL 240

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGI--TVTLKEAEPPMESPTGPFDFPESL 300
           S+EG+DFLRRCFVKDPRKRWTAEMLLNHPFVAG   TVTLKE+    ESPTGPFDFPE +
Sbjct: 241 SEEGKDFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLTTESPTGPFDFPEFI 300

Query: 301 PTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
              S+Q  ++   C S+ + E M+RLRR+VTEKPLDWS ++SWVTVR
Sbjct: 301 -CSSNQRCEEMSLCCSSSTAEMMNRLRRVVTEKPLDWSFTESWVTVR 346

[Close]

BLAST of MS009102 vs. ExPASy TrEMBL
Match: A0A6J1L7G1 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111499874 PE=3 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 8.3e-159
Identity = 270/347 (77.81%), Postives = 302/347 (87.03%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRG+ELGRGNF TI+LA  TKGFDQFPPLMAVKSSV+ S  S SLKNEK++LDRIGN
Sbjct: 1   MEWVRGDELGRGNFGTISLARLTKGFDQFPPLMAVKSSVSVSVSSASLKNEKKVLDRIGN 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQIV+C+GDGF++ERDGEKYYN+ LEYANGGSLAD+VK+HGGGL EFDVRRYT AIL G
Sbjct: 61  CPQIVTCYGDGFSVERDGEKYYNLFLEYANGGSLADTVKLHGGGLLEFDVRRYTRAILCG 120

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L+HVHGNGFVHCDLKL+NVL FGNG+VKIADFGLAK A      ETE+ FEWRGTPMYMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLVFGNGEVKIADFGLAKEAAAAETEETEENFEWRGTPMYMS 180

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PETV GG+Y PPCD+WALGCAVVEMV GKPAW V+PETDIFGLMM IG+GDEVPELPENL
Sbjct: 181 PETVNGGKYAPPCDVWALGCAVVEMVRGKPAWRVKPETDIFGLMMMIGIGDEVPELPENL 240

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGI--TVTLKEAEPPMESPTGPFDFPESL 300
           S+EG++FLRRCFVKDPRKRWTAEMLLNHPFVAG   TVTLKE+    ESPTGPFDFPE +
Sbjct: 241 SEEGKNFLRRCFVKDPRKRWTAEMLLNHPFVAGAGETVTLKESNLSTESPTGPFDFPEFV 300

Query: 301 PTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
              S+Q  D+   C S+ S E M+RLRRLVTEKPLDWSV++SWVTVR
Sbjct: 301 -CSSNQRCDEMNLCCSSSSAEMMNRLRRLVTEKPLDWSVTESWVTVR 346

[Close]

BLAST of MS009102 vs. ExPASy TrEMBL
Match: A0A0A0LMI4 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G278170 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.4e-158
Identity = 271/347 (78.10%), Postives = 303/347 (87.32%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EW+RG++LGRGNFATINLA  TKGFDQFPPLMAVKSSV++ S   SLKNEKQILDRIG 
Sbjct: 1   MEWIRGDQLGRGNFATINLAKLTKGFDQFPPLMAVKSSVSSLSSVSSLKNEKQILDRIGV 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQI++C+GDGF++E+DG+K YN+ LEYANGGSLAD+++IHGGGL EFDVRRYT AIL G
Sbjct: 61  CPQIITCYGDGFSVEKDGDKCYNLFLEYANGGSLADALRIHGGGLSEFDVRRYTRAILCG 120

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           LQHVHGNGFVHCDLKL+NVL FGNG+VKIADFGLAK AGK  A ETE+RFEWRGTPMYMS
Sbjct: 121 LQHVHGNGFVHCDLKLSNVLIFGNGEVKIADFGLAKSAGKFAAVETEERFEWRGTPMYMS 180

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PE V  GEYE PCDIWALGCAVVEMV GKPAW V PETD+FGLMMRIGVGDEVPE+PENL
Sbjct: 181 PEIVNDGEYESPCDIWALGCAVVEMVVGKPAWRVGPETDMFGLMMRIGVGDEVPEVPENL 240

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGI--TVTLKEAEPPMESPTGPFDFPESL 300
           S EG+DF+RRCFVKDP KRWTAE+LLNHPFVAG   TVTLKE E   ESPTGPFDFPE +
Sbjct: 241 SAEGKDFIRRCFVKDPSKRWTAEILLNHPFVAGAGDTVTLKEVELATESPTGPFDFPEFV 300

Query: 301 PTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
             CS Q SD+  FCSS+ SPE +SR+R+L+T KPLDWSV DSWVTVR
Sbjct: 301 --CSGQGSDEWSFCSSSSSPEVLSRVRQLMTGKPLDWSVMDSWVTVR 345

[Close]

BLAST of MS009102 vs. ExPASy TrEMBL
Match: A0A5A7TV84 (Mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00710 PE=3 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 1.7e-156
Identity = 272/349 (77.94%), Postives = 299/349 (85.67%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRG++LGRGNFATI+LA  TKGFDQFPPLMAVKSSV++ S SVSLKNEKQILDRIG 
Sbjct: 1   MEWVRGDQLGRGNFATISLAKLTKGFDQFPPLMAVKSSVSSLS-SVSLKNEKQILDRIGV 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
           CPQI++C+GDGF++E+DG+KYYN+ LEYANGGSLAD V+ HGGGL EFDVRRYT AIL G
Sbjct: 61  CPQIITCYGDGFSVEKDGDKYYNLFLEYANGGSLADVVRTHGGGLSEFDVRRYTRAILYG 120

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L+HVHGNGFVHCDLKL+NVL FGNG+VKIADFGLAK AGK  A ETE+RFEWRGTPMYMS
Sbjct: 121 LRHVHGNGFVHCDLKLSNVLIFGNGEVKIADFGLAKSAGKFGAVETEERFEWRGTPMYMS 180

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPENL 240
           PE V  GEYE PCDIWALGCAVVEMV GKPAW V P TD+FGLMMRIGVGDEVPE PENL
Sbjct: 181 PEIVNDGEYESPCDIWALGCAVVEMVVGKPAWRVGPGTDMFGLMMRIGVGDEVPETPENL 240

Query: 241 SDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAG----ITVTLKEAEPPMESPTGPFDFPE 300
           S EG+DF+RRCFVKDP KRWTAEMLLNHPFV       TVTLKE E   ESPTGPFDFPE
Sbjct: 241 SAEGKDFIRRCFVKDPSKRWTAEMLLNHPFVVAGAGDTTVTLKEVELTTESPTGPFDFPE 300

Query: 301 SLPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
            +  CS Q SD+  FCSS+ SPE +SR+R LVT KPLDWSV DSWVTVR
Sbjct: 301 FV--CSGQGSDEWSFCSSSSSPEVLSRVRHLVTGKPLDWSVMDSWVTVR 346

[Close]

BLAST of MS009102 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 349.4 bits (895), Expect = 3.4e-96
Identity = 179/348 (51.44%), Postives = 238/348 (68.39%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQ-FPPLMAVKSSVAASSVSVSLKNEKQILDRIG 60
           +EW+R E +G G+F+T++LA  +    + FP LMAVKSS      S +L+NE+ +LD +G
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVV--CSAALRNERDVLDDLG 60

Query: 61  NCPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILR 120
           +C +IV CFG+G T+E +GE+ YN+ LEYA+GGSLAD +K  G  LPEF+VRR+T +I++
Sbjct: 61  DCSEIVRCFGEGRTVE-NGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVK 120

Query: 121 GLQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYM 180
           GL H+HGNGF HCD+KL NVL FG+GDVKI+DFGLAK    +   E       RGTP+YM
Sbjct: 121 GLCHIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKRRSGEVCVEI------RGTPLYM 180

Query: 181 SPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRP--ETDIFGLMMRIGVGDEVPELP 240
           +PE+V  GE+E P DIWALGC+VVEM +GK AW +      ++  L++RIG GDEVP +P
Sbjct: 181 APESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIP 240

Query: 241 ENLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVAGITVTLKEAEPPMESPTGPFDFPES 300
             LS+EG+DF+ +CFVK+  +RWTAEMLL+HPF+A    + +E E    SP  PFDFP  
Sbjct: 241 VELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEEDEACSVSPRNPFDFP-G 300

Query: 301 LPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
             +     +D   F S   SPE   R+  LV+EK  DWSVS  WV VR
Sbjct: 301 WNSVQSPVNDSVMFGSLVGSPE--ERISGLVSEKVPDWSVSCDWVNVR 336

[Close]

BLAST of MS009102 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 340.5 bits (872), Expect = 1.6e-93
Identity = 178/357 (49.86%), Postives = 237/357 (66.39%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           +EWVRGE +G G F+T++ A  ++    FP L+AVKS+ A  + S+S  NEK +LD +G+
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLS--NEKSVLDSLGD 60

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGG-GLPEFDVRRYTAAILR 120
           CP+I+ C+G+  T+E +GE+ +N+LLEYA+ GSLA  +K  GG GLPE  VRR+T ++LR
Sbjct: 61  CPEIIRCYGEDSTVE-NGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLR 120

Query: 121 GLQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLA-KVAGKKTAAETEQRFEWRGTPMY 180
           GL+H+H  GF HCD+KLAN+L F +G VKIADFGLA +V G  TA    +  E RGTP+Y
Sbjct: 121 GLRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTA--LRKSVEIRGTPLY 180

Query: 181 MSPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELPE 240
           M+PE V   EY    D+WALGCAVVEM +GK AW+V+  +    L++RIGVGDE+P++PE
Sbjct: 181 MAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPE 240

Query: 241 NLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFVA---------GITVTLKEAEPPMESPT 300
            LS+EG+DFL +CFVKDP KRWTAEMLLNH FV             V +K+ +  + SP 
Sbjct: 241 MLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPK 300

Query: 301 GPFDFPE-SLPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
            PF+F +    T    PS DS           + RL  LV+    DWSV  SW+TVR
Sbjct: 301 CPFEFDDWDSFTLDSNPSFDS----------PVERLGSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of MS009102 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 339.3 bits (869), Expect = 3.5e-93
Identity = 182/359 (50.70%), Postives = 240/359 (66.85%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKG-FDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIG 60
           +EW+RGE +G G F+T++LA  +     +FPPLMAVKS  A S  + SL NEK +LD +G
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKS--ADSYGAASLANEKSVLDNLG 60

Query: 61  -NCPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSV-KIHGGGLPEFDVRRYTAAI 120
            +C +IV CFG+  T+E +GE+ +N+ LEYA+ GSL   + K+ G G+PE  VRR+T ++
Sbjct: 61  DDCNEIVRCFGEDRTVE-NGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSV 120

Query: 121 LRGLQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPM 180
           LRGL+H+H NGF HCDLKL N+L FG+G VKIADFGLAK  G  TA       + RGTP+
Sbjct: 121 LRGLRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIGDLTA--LNYGVQIRGTPL 180

Query: 181 YMSPETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPETDIFGLMMRIGVGDEVPELP 240
           YM+PE+V   EY    D+WALGC VVEM +GK AW+++  ++   L++RIGVGDEVP +P
Sbjct: 181 YMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIP 240

Query: 241 ENLSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFV---AGITVTLKE--------AEPPME 300
           E LS++GRDFL +CFVKDP+KRWTAEMLLNHPFV       V +KE         E    
Sbjct: 241 EELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVST 300

Query: 301 SPTGPFDFPESLPTCSDQPSDDSYFCSSTVSPETMSRLRRLVTEKPLDWSVSDSWVTVR 346
           SP  PF+FP+ +   S   + D        SP+   R+  LVT+   DWSV++SWVTVR
Sbjct: 301 SPRCPFEFPDWVSVSSGSQTID--------SPD--ERVASLVTDMIPDWSVTNSWVTVR 344

[Close]

BLAST of MS009102 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 218.0 bits (554), Expect = 1.2e-56
Identity = 118/272 (43.38%), Postives = 159/272 (58.46%), Query Frame = 0


Query: 1   LEWVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGN 60
           + W RG  +GRG+ AT+++AI + G      L AVKS  A  S S  L+ E+ IL  + +
Sbjct: 3   INWTRGPIIGRGSTATVSIAISSSG-----ELFAVKS--ADLSSSSLLQKEQSILSTLSS 62

Query: 61  CPQIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRG 120
            P +V   G G T E +G   YN+L+EY +GG+L D +K  GG LPE ++R YT  IL G
Sbjct: 63  -PHMVKYIGTGLTRESNG-LVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNG 122

Query: 121 LQHVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMS 180
           L ++H  G VHCDLK  NVL   NG +KIAD G AK   K          E+ GTP +M+
Sbjct: 123 LVYLHERGIVHCDLKSHNVLVEENGVLKIADMGCAKSVDKS---------EFSGTPAFMA 182

Query: 181 PETVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPE-TDIFGLMMRIGVGDEVPELPEN 240
           PE   G E   P D+WALGC ++EM+ G   W   PE  D+   M +IG   E P +P  
Sbjct: 183 PEVARGEEQRFPADVWALGCTMIEMMTGSSPW---PELNDVVAAMYKIGFSGESPAIPAW 242

Query: 241 LSDEGRDFLRRCFVKDPRKRWTAEMLLNHPFV 272
           +SD+ +DFL+ C  +D ++RWT E LL HPF+
Sbjct: 243 ISDKAKDFLKNCLKEDQKQRWTVEELLKHPFL 253

[Close]

BLAST of MS009102 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 217.6 bits (553), Expect = 1.5e-56
Identity = 119/270 (44.07%), Postives = 156/270 (57.78%), Query Frame = 0


Query: 3   WVRGEELGRGNFATINLAIPTKGFDQFPPLMAVKSSVAASSVSVSLKNEKQILDRIGNCP 62
           W+RG  +GRG+ AT++L I   G        AVKS  A  S S  L+ E+ IL ++ + P
Sbjct: 6   WIRGPIIGRGSTATVSLGITNSG-----DFFAVKS--AEFSSSAFLQREQSILSKLSS-P 65

Query: 63  QIVSCFGDGFTIERDGEKYYNVLLEYANGGSLADSVKIHGGGLPEFDVRRYTAAILRGLQ 122
            IV   G   T E D +  YN+L+EY +GGSL D +K  GG LPE  +R YT  IL+GL 
Sbjct: 66  YIVKYIGSNVTKEND-KLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLM 125

Query: 123 HVHGNGFVHCDLKLANVLNFGNGDVKIADFGLAKVAGKKTAAETEQRFEWRGTPMYMSPE 182
           ++H  G VHCD+K  NV+  G    KI D G AK        E  +  E+ GTP +MSPE
Sbjct: 126 YLHDQGIVHCDVKSQNVM-IGGEIAKIVDLGCAK------TVEENENLEFSGTPAFMSPE 185

Query: 183 TVTGGEYEPPCDIWALGCAVVEMVAGKPAWNVRPE-TDIFGLMMRIGVGDEVPELPENLS 242
              G E   P D+WALGC V+EM  G   W   PE  D+   + +IG   E P +P  LS
Sbjct: 186 VARGEEQSFPADVWALGCTVIEMATGSSPW---PELNDVVAAIYKIGFTGESPVIPVWLS 245

Query: 243 DEGRDFLRRCFVKDPRKRWTAEMLLNHPFV 272
           ++G+DFLR+C  KDP++RWT E LL HPF+
Sbjct: 246 EKGQDFLRKCLRKDPKQRWTVEELLQHPFL 256

The following BLAST results are available for this feature:

BLAST of MS009102 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022149480.1	8.7e-203	99.42	mitogen-activated protein kinase kinase kinase 18-like [Momordica charantia]	[more]
KAG6605205.1	1.4e-160	78.67	Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper...	[more]
XP_022948206.1	1.5e-159	78.10	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata]	[more]
XP_038902011.1	7.7e-159	80.46	mitogen-activated protein kinase kinase kinase 18-like isoform X1 [Benincasa his...	[more]
XP_023007353.1	1.7e-158	77.81	mitogen-activated protein kinase kinase kinase 18-like [Cucurbita maxima]	[more]

BLAST of MS009102 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	2.2e-92	49.86	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	2.0e-48	37.75	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q9ZVP5	4.8e-47	36.31	Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370...	[more]
Q99759	7.0e-38	36.33	Mitogen-activated protein kinase kinase kinase 3 OS=Homo sapiens OX=9606 GN=MAP3...	[more]
Q61084	1.2e-37	36.33	Mitogen-activated protein kinase kinase kinase 3 OS=Mus musculus OX=10090 GN=Map...	[more]

BLAST of MS009102 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5U5	4.2e-203	99.42	mitogen-activated protein kinase kinase kinase 18-like OS=Momordica charantia OX...	[more]
A0A6J1G8M2	7.5e-160	78.10	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=...	[more]
A0A6J1L7G1	8.3e-159	77.81	mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=36...	[more]
A0A0A0LMI4	1.4e-158	78.10	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G278...	[more]
A0A5A7TV84	1.7e-156	77.94	Mitogen-activated protein kinase kinase kinase 1-like OS=Cucumis melo var. makuw...	[more]

BLAST of MS009102 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	3.4e-96	51.44	mitogen-activated protein kinase kinase kinase 21	[more]
AT3G50310.1	1.6e-93	49.86	mitogen-activated protein kinase kinase kinase 20	[more]
AT5G67080.1	3.5e-93	50.70	mitogen-activated protein kinase kinase kinase 19	[more]
AT4G26890.1	1.2e-56	43.38	mitogen-activated protein kinase kinase kinase 16	[more]
AT5G55090.1	1.5e-56	44.07	mitogen-activated protein kinase kinase kinase 15	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 3..271 e-value: 2.9E-63 score: 226.2
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 5..271 e-value: 4.0E-50 score: 170.7
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 3..271 score: 37.361122
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 1..95 e-value: 7.7E-7 score: 31.3
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 96..289 e-value: 3.8E-51 score: 175.8
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 2..308
None	No IPR available	PANTHER	PTHR48011:SF18	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20	coord: 2..308
None	No IPR available	CDD	cd06606	STKc_MAPKKK	coord: 2..271 e-value: 1.28589E-102 score: 300.591
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 9..36
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009102.1	MS009102.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity