MS009092 (gene) Bitter gourd (TR) v1

Overview


Name	MS009092
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RNase H domain-containing protein
Location	scaffold687: 596946 .. 597299 (-)
RNA-Seq Expression	MS009092
Synteny	MS009092

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCCGGCGAAGCACAATTGAAGGGAGCAGTTGGACCACGCCACCACACCATAGATGGAAATTGAACACGGACGCTACCTGGATGGACTCCCTGAATCGTGGTGGCATCGGCTGGATTCTCCGCGACTCTAATGGCTCGATGATTTCGACTGGGTGTAGGCTTGTGCATCCCAGATGGCAAAGCAAGGTTTTGGAGGCGATGGCTGTGGTTGAAGGTCTGAAATCTATTAACAAGGAAGAAATGTTAGAAACCATTGTTGAGTCGGACTGCCTTGAACTAGTTAATCTGCTGAAAGGGGTGAATTCCGACCTAACTGAGATTAGCTTTTCATCGACGAAGCTTTGCGTCTACAAG

mRNA sequence

GTCCGGCGAAGCACAATTGAAGGGAGCAGTTGGACCACGCCACCACACCATAGATGGAAATTGAACACGGACGCTACCTGGATGGACTCCCTGAATCGTGGTGGCATCGGCTGGATTCTCCGCGACTCTAATGGCTCGATGATTTCGACTGGGTGTAGGCTTGTGCATCCCAGATGGCAAAGCAAGGTTTTGGAGGCGATGGCTGTGGTTGAAGGTCTGAAATCTATTAACAAGGAAGAAATGTTAGAAACCATTGTTGAGTCGGACTGCCTTGAACTAGTTAATCTGCTGAAAGGGGTGAATTCCGACCTAACTGAGATTAGCTTTTCATCGACGAAGCTTTGCGTCTACAAG

Coding sequence (CDS)

GTCCGGCGAAGCACAATTGAAGGGAGCAGTTGGACCACGCCACCACACCATAGATGGAAATTGAACACGGACGCTACCTGGATGGACTCCCTGAATCGTGGTGGCATCGGCTGGATTCTCCGCGACTCTAATGGCTCGATGATTTCGACTGGGTGTAGGCTTGTGCATCCCAGATGGCAAAGCAAGGTTTTGGAGGCGATGGCTGTGGTTGAAGGTCTGAAATCTATTAACAAGGAAGAAATGTTAGAAACCATTGTTGAGTCGGACTGCCTTGAACTAGTTAATCTGCTGAAAGGGGTGAATTCCGACCTAACTGAGATTAGCTTTTCATCGACGAAGCTTTGCGTCTACAAG

Protein sequence

VRRSTIEGSSWTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAMAVVEGLKSINKEEMLETIVESDCLELVNLLKGVNSDLTEISFSSTKLCVYK

Homology

[Close]

BLAST of MS009092 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 91.7 bits (226), Expect = 4.7e-15
Identity = 44/100 (44.00%), Postives = 57/100 (57.00%), Query Frame = 0


Query: 8   GSSWTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAM 67
           G+ W  P  + WKLNT+A W    N GGIGWILRD  G +I   CR++        LE M
Sbjct: 76  GAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVM 135

Query: 68  AVVEGLKSINKEEMLETIVESDCLELVNLLKGVNSDLTEI 108
           A+ EGL++I +E      +ESD LE ++LL     D TEI
Sbjct: 136 AICEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 175

[Close]

BLAST of MS009092 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 88.2 bits (217), Expect = 5.2e-14
Identity = 41/87 (47.13%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 11  WTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAMAVV 70
           W  P  + WKLNTDA W    N  GIGWILRD  G +I TGCR++        LE MA+ 
Sbjct: 255 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 314

Query: 71  EGLKSINKEEMLETIVESDCLELVNLL 98
           EGL++I +E      +ESD LE ++LL
Sbjct: 315 EGLRAIRQEHCRPIHLESDSLEAIHLL 341

[Close]

BLAST of MS009092 vs. NCBI nr
Match: XP_022154991.1 (uncharacterized protein LOC111022134 isoform X2 [Momordica charantia])

HSP 1 Score: 88.2 bits (217), Expect = 5.2e-14
Identity = 41/87 (47.13%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 11  WTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAMAVV 70
           W  P  + WKLNTDA W    N  GIGWILRD  G +I TGCR++        LE MA+ 
Sbjct: 220 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 279

Query: 71  EGLKSINKEEMLETIVESDCLELVNLL 98
           EGL++I +E      +ESD LE ++LL
Sbjct: 280 EGLRAIRQEHCRPIHLESDSLEAIHLL 306

[Close]

BLAST of MS009092 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 86.3 bits (212), Expect = 2.0e-13
Identity = 47/108 (43.52%), Postives = 59/108 (54.63%), Query Frame = 0


Query: 8   GSSWTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAM 67
           G+ W  P  + WKLNTDA W    N GGIGWILRD  G +I   CR++        LE M
Sbjct: 76  GARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVM 135

Query: 68  AVVEGLKSINKE-------EMLETI-VESDCLELVNLLKGVNSDLTEI 108
           A+ EGL++I +E       E    I +ESD LE ++LL     D TEI
Sbjct: 136 AICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 183

[Close]

BLAST of MS009092 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 83.6 bits (205), Expect = 1.3e-12
Identity = 41/97 (42.27%), Postives = 60/97 (61.86%), Query Frame = 0


Query: 11  WTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAMAVV 70
           W  PP H W LN DA+W DS +RGGIGWI+R  +G ++  G R V      K+LEA A++
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 71  EGLKSINKEEMLETI-VESDCLELVNLLKGVNSDLTE 107
           EGL+++    +L  + +E+D  E+ +LL     DLT+
Sbjct: 131 EGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTK 167

[Close]

BLAST of MS009092 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 2.3e-15
Identity = 44/100 (44.00%), Postives = 57/100 (57.00%), Query Frame = 0


Query: 8   GSSWTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAM 67
           G+ W  P  + WKLNT+A W    N GGIGWILRD  G +I   CR++        LE M
Sbjct: 76  GAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVM 135

Query: 68  AVVEGLKSINKEEMLETIVESDCLELVNLLKGVNSDLTEI 108
           A+ EGL++I +E      +ESD LE ++LL     D TEI
Sbjct: 136 AICEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 175

[Close]

BLAST of MS009092 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.5e-14
Identity = 41/87 (47.13%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 11  WTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAMAVV 70
           W  P  + WKLNTDA W    N  GIGWILRD  G +I TGCR++        LE MA+ 
Sbjct: 255 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 314

Query: 71  EGLKSINKEEMLETIVESDCLELVNLL 98
           EGL++I +E      +ESD LE ++LL
Sbjct: 315 EGLRAIRQEHCRPIHLESDSLEAIHLL 341

[Close]

BLAST of MS009092 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 2.5e-14
Identity = 41/87 (47.13%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 11  WTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAMAVV 70
           W  P  + WKLNTDA W    N  GIGWILRD  G +I TGCR++        LE MA+ 
Sbjct: 220 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 279

Query: 71  EGLKSINKEEMLETIVESDCLELVNLL 98
           EGL++I +E      +ESD LE ++LL
Sbjct: 280 EGLRAIRQEHCRPIHLESDSLEAIHLL 306

[Close]

BLAST of MS009092 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 9.5e-14
Identity = 47/108 (43.52%), Postives = 59/108 (54.63%), Query Frame = 0


Query: 8   GSSWTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAM 67
           G+ W  P  + WKLNTDA W    N GGIGWILRD  G +I   CR++        LE M
Sbjct: 76  GARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVM 135

Query: 68  AVVEGLKSINKE-------EMLETI-VESDCLELVNLLKGVNSDLTEI 108
           A+ EGL++I +E       E    I +ESD LE ++LL     D TEI
Sbjct: 136 AICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEI 183

[Close]

BLAST of MS009092 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.2e-13
Identity = 41/97 (42.27%), Postives = 60/97 (61.86%), Query Frame = 0


Query: 11  WTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEAMAVV 70
           W  PP H W LN DA+W DS +RGGIGWI+R  +G ++  G R V      K+LEA A++
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 71  EGLKSINKEEMLETI-VESDCLELVNLLKGVNSDLTE 107
           EGL+++    +L  + +E+D  E+ +LL     DLT+
Sbjct: 131 EGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTK 167

[Close]

BLAST of MS009092 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 56.2 bits (134), Expect = 2.0e-08
Identity = 40/95 (42.11%), Postives = 54/95 (56.84%), Query Frame = 0


Query: 11  WTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEA--MA 70
           W  PP+   K NTDATW     R GIGWILR+ +G ++  G R + PR ++ VLEA   A
Sbjct: 137 WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARAL-PRTKN-VLEAELEA 196

Query: 71  VVEGLKSINKEEMLETIVESDCLELVNLLKGVNSD 104
           +   + ++++      I ESD   LVNLL   NSD
Sbjct: 197 LRWAVLTMSRFNYKRIIFESDAQALVNLL---NSD 226

[Close]

BLAST of MS009092 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 2.2e-07
Identity = 35/89 (39.33%), Postives = 49/89 (55.06%), Query Frame = 0


Query: 11  WTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSKVLEA--MA 70
           W  PPH   K NTDATW     R GIGW+LR+  G +   G R + P+ +S VLEA   A
Sbjct: 420 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARAL-PKLKS-VLEAELEA 479

Query: 71  VVEGLKSINKEEMLETIVESDCLELVNLL 98
           +   + S+++ +    I ESD   L+ +L
Sbjct: 480 MRWAVLSLSRFQYNYVIFESDSQVLIEIL 506

[Close]

BLAST of MS009092 vs. TAIR 10
Match: AT4G09775.1 (BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily protein (TAIR:AT2G02650.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 44.3 bits (103), Expect = 8.0e-05
Identity = 20/74 (27.03%), Postives = 36/74 (48.65%), Query Frame = 0


Query: 3   RSTIEGSSWTTPPHHRWKLNTDATWMDSLNRGGIGWILRDSNGSMISTGCRLVHPRWQSK 62
           R +     W+ PP    K N D+ ++   +     WI+RDSNG +I +GC  +   + + 
Sbjct: 54  RPSGRSKEWSPPPEGYLKCNFDSGYVQGRDYTSTCWIIRDSNGHVIHSGCAKLQQSYSAL 113

Query: 63  VLEAMAVVEGLKSI 77
             EA+  +  L+ +
Sbjct: 114 QAEALGFLHALQMV 127

The following BLAST results are available for this feature:

BLAST of MS009092 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143535.1	4.7e-15	44.00	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022154990.1	5.2e-14	47.13	uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]	[more]
XP_022154991.1	5.2e-14	47.13	uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]	[more]
XP_022156777.1	2.0e-13	43.52	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022155262.1	1.3e-12	42.27	uncharacterized protein LOC111022403 [Momordica charantia]	[more]

BLAST of MS009092 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS009092 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP26	2.3e-15	44.00	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DL64	2.5e-14	47.13	uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DQC9	2.5e-14	47.13	uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DSV1	9.5e-14	43.52	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DNV9	6.2e-13	42.27	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]

BLAST of MS009092 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	2.0e-08	42.11	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	2.2e-07	39.33	Ribonuclease H-like superfamily protein	[more]
AT4G09775.1	8.0e-05	27.03	BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily prot...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 16..114 e-value: 5.4E-9 score: 38.2
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 22..102 e-value: 1.9E-15 score: 56.8
None	No IPR available	PANTHER	PTHR47074	BNAC02G40300D PROTEIN	coord: 5..111
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 21..99 e-value: 1.16453E-12 score: 57.7092
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 19..99

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS009092.1	MS009092.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity