Homology
BLAST of MS009088 vs. NCBI nr
Match:
XP_022149455.1 (cytochrome P450 81E8-like [Momordica charantia])
HSP 1 Score: 961.4 bits (2484), Expect = 2.8e-276
Identity = 470/473 (99.37%), Postives = 473/473 (100.00%), Query Frame = 0
Query: 1 RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSA 60
RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSA
Sbjct: 24 RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSA 83
Query: 61 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 120
VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF
Sbjct: 84 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 143
Query: 121 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFRE 180
LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFRE
Sbjct: 144 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFRE 203
Query: 181 MVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNT 240
MVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNT
Sbjct: 204 MVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNT 263
Query: 241 MIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAE 300
MIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAE
Sbjct: 264 MIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAE 323
Query: 301 IDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTI 360
IDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTI
Sbjct: 324 IDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTI 383
Query: 361 ILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLT 420
ILIN+WAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLT
Sbjct: 384 ILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLT 443
Query: 421 LASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEAII 474
LASLIQGFEWERMSNSLVDM+EGKGLTMPKAQHLVAKCKPRP+MKLILNEAII
Sbjct: 444 LASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAKCKPRPIMKLILNEAII 496
BLAST of MS009088 vs. NCBI nr
Match:
XP_022149460.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])
HSP 1 Score: 774.2 bits (1998), Expect = 6.3e-220
Identity = 370/474 (78.06%), Postives = 427/474 (90.08%), Query Frame = 0
Query: 1 RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSA 60
R R+NLPPSP F LP++GHLHLVKHPVH+T H+LSH YGHVFSLR+GSR+VVVVSSPSA
Sbjct: 104 RPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSA 163
Query: 61 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 120
VQECFTKNDIVLANRPLLKSGK LAYNHTT+AV+PYGEHWRNLRRI TLEIFST+RLN+F
Sbjct: 164 VQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLF 223
Query: 121 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY-----EEQQS 180
LGIREDEV+NL+ KL +S +EF AVEL+ ML D TFNIV+RM+AGKKYY +E QS
Sbjct: 224 LGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQS 283
Query: 181 KKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQE 240
KKFREMV ++LA+ G N GDF+P+WNWIDPTG+EKRI+K+G+RSDELLQEL+DEIRNQ+
Sbjct: 284 KKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQK 343
Query: 241 GGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLE 300
G NTMIDHLL LQ +PEY+SDQIIKGL Q++LLAGIDTAA+TLEWALS+LLNNPEVL+
Sbjct: 344 DGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLK 403
Query: 301 KAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDV 360
KA+AEID F+G++RLV+E DLSSL YLQG+I ETLRLNPAAPLL+PHCAS DCKI GYD+
Sbjct: 404 KARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDI 463
Query: 361 PRDTIILINVWAIHRDPNLWEDATNFKPERHGNTI-GVDSYKLLPFGSGRRSCPGVGMAQ 420
PRDTIILIN WAIHRD N+WEDAT+FKPERH NT+ GVDSYKLLPFG GRR+CPG+GMAQ
Sbjct: 464 PRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQ 523
Query: 421 RVVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLIL 469
RVVGLTLASLIQ FEWER+++SLVDM+EG+GLTMPKAQ LVAKCKPRP++K IL
Sbjct: 524 RVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAIL 577
BLAST of MS009088 vs. NCBI nr
Match:
XP_022947035.1 (cytochrome P450 81F3 [Cucurbita moschata])
HSP 1 Score: 761.1 bits (1964), Expect = 5.5e-216
Identity = 360/469 (76.76%), Postives = 416/469 (88.70%), Query Frame = 0
Query: 4 RQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQE 63
R NLPP+PLFSLP++GH HL+KHP+H++ H LS K+GHV SLRFGSR+VVV+SSPSAVQE
Sbjct: 27 RLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQKHGHVISLRFGSRLVVVISSPSAVQE 86
Query: 64 CFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLGI 123
CFTKNDI+LANRPLL +GK LAYNHTT+AV+PYGEHWRNLRRI +LEIFST RLNMF+GI
Sbjct: 87 CFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNLRRICSLEIFSTNRLNMFVGI 146
Query: 124 REDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFREMVT 183
RE+EV+ L+ KLC + EEF VELEPML DLTFN+VM MV G+++Y+E SKKFR++VT
Sbjct: 147 RENEVKRLLRKLCGDWSEEFREVELEPMLSDLTFNVVMGMVGGRRFYDEGYSKKFRDLVT 206
Query: 184 QVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMID 243
Q++AH G++NPGDFIP+WNW+DPTGF+KRI+ L +R DELLQELIDE R+ + G NTMI
Sbjct: 207 QIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDELLQELIDEARDGKDGGNTMIH 266
Query: 244 HLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDC 303
HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+AVTLEWALS LLNNP+VL+KA+AEID
Sbjct: 267 HLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSAVTLEWALSHLLNNPDVLKKARAEIDD 326
Query: 304 FVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILI 363
+G+ERLVNE+DL +L YLQGII ETLRLNPAAPLL+PHCA DCKI GYDVPRDTI+LI
Sbjct: 327 LIGKERLVNESDLPNLKYLQGIISETLRLNPAAPLLVPHCAFQDCKIEGYDVPRDTIVLI 386
Query: 364 NVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLAS 423
N WAIHRDPNLWED T FKPERHG+T G DSYKLLPFG GRR+CPGVGMAQRVVGL LAS
Sbjct: 387 NTWAIHRDPNLWEDPTAFKPERHGDTSGADSYKLLPFGLGRRACPGVGMAQRVVGLALAS 446
Query: 424 LIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEAI 473
LIQGFEWER+S+SLVDMSEG+GLTMPKAQ LVAKCKPRP+MK I+N I
Sbjct: 447 LIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAKCKPRPIMKAIMNVEI 495
BLAST of MS009088 vs. NCBI nr
Match:
XP_023532689.1 (cytochrome P450 81F3-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532690.1 cytochrome P450 81F3-like isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 756.9 bits (1953), Expect = 1.0e-214
Identity = 357/469 (76.12%), Postives = 415/469 (88.49%), Query Frame = 0
Query: 4 RQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQE 63
R NLPP+PLFSLP++GH HL+KHP+H++ H LS K+GHV SLRFGSR+VVV+SSPSAVQE
Sbjct: 27 RLNLPPTPLFSLPLIGHFHLLKHPLHRSLHTLSQKHGHVISLRFGSRLVVVISSPSAVQE 86
Query: 64 CFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLGI 123
CFTKNDI+LANRPLL +GK LAYNHTT+A++PYGEHWRNLRRI +LEIFST RLNMF+GI
Sbjct: 87 CFTKNDIILANRPLLDTGKYLAYNHTTLAISPYGEHWRNLRRICSLEIFSTNRLNMFVGI 146
Query: 124 REDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFREMVT 183
RE+EV+ L+ KLC EE VELEPML DLTFN+VM MV G+++Y+E SKKFR++VT
Sbjct: 147 RENEVKRLLRKLCGGWLEEGREVELEPMLSDLTFNVVMGMVGGRRFYDEGYSKKFRDLVT 206
Query: 184 QVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMID 243
Q++AH G++NPGDFIP+WNW+DPTGF+KRI+ L +R DE+LQELIDE R+ + G NTMI
Sbjct: 207 QIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDEVLQELIDEARDGKDGGNTMIH 266
Query: 244 HLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDC 303
HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+AVTLEWALS+LLNNP+VL+KA+AEID
Sbjct: 267 HLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSAVTLEWALSQLLNNPDVLKKARAEIDD 326
Query: 304 FVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILI 363
+G+ERLVNE+DL +L YLQGII ETLRLNPAAPLL+PHCA DCKI GYDVPRDTI+LI
Sbjct: 327 LIGKERLVNESDLPNLKYLQGIISETLRLNPAAPLLVPHCAFQDCKIEGYDVPRDTIVLI 386
Query: 364 NVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLAS 423
N WAIHRDPNLWED T FKPERHG+T G DSYKLLPFG GRR+CPGVGMAQRVVGL LAS
Sbjct: 387 NAWAIHRDPNLWEDPTAFKPERHGDTSGADSYKLLPFGLGRRACPGVGMAQRVVGLALAS 446
Query: 424 LIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEAI 473
LIQGFEWER+S+SLVDMSEG+GLTMPKAQ LVAKCKPRP+MK I+N I
Sbjct: 447 LIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAKCKPRPIMKAIMNVEI 495
BLAST of MS009088 vs. NCBI nr
Match:
XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])
HSP 1 Score: 753.8 bits (1945), Expect = 8.8e-214
Identity = 353/469 (75.27%), Postives = 417/469 (88.91%), Query Frame = 0
Query: 4 RQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQE 63
R NLPP+PLFSLP++GH HL+KHP+H++ H LS K+GHV SLRFGSR+VV++SSPSAVQE
Sbjct: 27 RLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQKHGHVISLRFGSRLVVLISSPSAVQE 86
Query: 64 CFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLGI 123
CFTKNDI+LANRPLL +GK LAYNHTT+A++PYGEHWRNLRRI +LEIFST+RLN+FLGI
Sbjct: 87 CFTKNDIILANRPLLDTGKYLAYNHTTLAISPYGEHWRNLRRICSLEIFSTSRLNLFLGI 146
Query: 124 REDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFREMVT 183
R++EV+ L+ KLC + EEF VE+EPML DLTFN+VM MV G+++Y+E SKKFR++VT
Sbjct: 147 RQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTFNVVMGMVGGRRFYDEGYSKKFRDLVT 206
Query: 184 QVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMID 243
Q++AH G++NPGDFIP+WNW+DPTGF+KRI+ L +R DELLQELIDE R+ + G N+MI
Sbjct: 207 QIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDELLQELIDEARDGKDGGNSMIH 266
Query: 244 HLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDC 303
HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+AVTLEWALS LLNNP+VL+KA+AEID
Sbjct: 267 HLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSAVTLEWALSHLLNNPDVLKKARAEIDD 326
Query: 304 FVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILI 363
+G+ERLVNE+DL +L YLQGII ETLRLNPAAPLL+PHCA DCKI GYDVPRDTI+LI
Sbjct: 327 LIGKERLVNESDLPNLRYLQGIISETLRLNPAAPLLVPHCAFQDCKIEGYDVPRDTIVLI 386
Query: 364 NVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLAS 423
N WAIHRDP+LWED T FKPERHG++ G DSYKLLPFG GRR+CPGVGMAQRVVGL LAS
Sbjct: 387 NAWAIHRDPDLWEDPTAFKPERHGDSSGADSYKLLPFGLGRRACPGVGMAQRVVGLALAS 446
Query: 424 LIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEAI 473
LIQGFEWER+S+SLVDMSEG+GLTMPKAQ LVAKCKPRP+MK I+N I
Sbjct: 447 LIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAKCKPRPIMKAIMNVEI 495
BLAST of MS009088 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 570.1 bits (1468), Expect = 2.4e-161
Identity = 271/472 (57.42%), Postives = 357/472 (75.64%), Query Frame = 0
Query: 3 RRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQ 62
RR+NLPPSP +LP++GHLHL+ +H++ ++LS KYG VFSL+ G+R+V+VVSSP+A +
Sbjct: 28 RRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAE 87
Query: 63 ECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLG 122
ECFTKNDIV ANRPL GK + YN+TTM +PYGEHWRNLRR+ +EIFS LN FL
Sbjct: 88 ECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLS 147
Query: 123 IREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY----EEQQSKKF 182
IREDEV+ L+L L ++S ++F VE++ L +L+FN+ MRMVAGK+Y+ + ++K F
Sbjct: 148 IREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDVDSDEAKLF 207
Query: 183 REMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGD 242
R ++ +V H GA+NPGDF+P WID +EK++ K+ Q D LQ LI E R + +
Sbjct: 208 RALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSKISQEMDAFLQRLIHESRINK-NN 267
Query: 243 NTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAK 302
TMIDHLL LQ+++PEYY+DQIIKG+I V+LLAG DT+AVT+EWA+S LLN+PE LEKA+
Sbjct: 268 VTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKAR 327
Query: 303 AEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRD 362
EI+ VG RL+ E DL L YL II ET RL PAAP+L+PH +S DCK+ GYDVP+
Sbjct: 328 TEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKG 387
Query: 363 TIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVG 422
TI+L+N WAIHRDP W++ T FKPERHG + ++ KL+PFG GRRSCPG G+AQRVVG
Sbjct: 388 TILLVNAWAIHRDPEFWDEPTLFKPERHGG-VELEPSKLMPFGMGRRSCPGSGLAQRVVG 447
Query: 423 LTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNE 471
LTL +LIQ FEW+R+ + +DM+EG GLTMPKAQ L A CKPR ++ +++E
Sbjct: 448 LTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRNILHKVVSE 497
BLAST of MS009088 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 522.3 bits (1344), Expect = 5.6e-147
Identity = 256/469 (54.58%), Postives = 340/469 (72.49%), Query Frame = 0
Query: 5 QNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQEC 64
+NLPP P LPI+G+LH +K P+H TFH LS KYG +FSL FGSR+VVVVSS + QEC
Sbjct: 30 KNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQEC 89
Query: 65 FTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLGIR 124
FTKNDIVLANRP +GK + YN+TT+A +PYG+HWRNLRRI ++EI S+ RLN FL IR
Sbjct: 90 FTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIR 149
Query: 125 EDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY-------EEQQSKK 184
DE+ L+ KL + S+ FT VEL PM ++TFN +MRMV+GK+YY + ++++
Sbjct: 150 RDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARL 209
Query: 185 FREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGG 244
FR ++ +V++ GA N GDF+ W D G EKR+ K+ +R+D LQ LIDE R +
Sbjct: 210 FRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRN 269
Query: 245 DNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKA 304
NTMIDHLL Q+++PEYY+DQIIKGL+ V+LLAG DT++VT+EWA+S LLN+PE+++KA
Sbjct: 270 SNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKA 329
Query: 305 KAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPR 364
K E+D +G +R V+E D+S L YLQ I++ETLRL+ AAPLL+PH +S D +GGY++P+
Sbjct: 330 KNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQ 389
Query: 365 DTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVV 424
+TI+++N W IHRDPNLW D T FKPER V+ KLL FG GRR+CPG ++QR
Sbjct: 390 NTILMVNAWVIHRDPNLWSDPTCFKPERFEKEGEVN--KLLSFGLGRRACPGENLSQRTE 449
Query: 425 GLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKL 467
GLTL LIQ FEW+R+ +DM E KG+T K L A CK R +K+
Sbjct: 450 GLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLNAMCKVRHPLKI 495
BLAST of MS009088 vs. ExPASy Swiss-Prot
Match:
Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)
HSP 1 Score: 516.5 bits (1329), Expect = 3.1e-145
Identity = 264/480 (55.00%), Postives = 343/480 (71.46%), Query Frame = 0
Query: 1 RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH-----VFSLRFGSRVVVVV 60
+ ++QNLPPSP LPI+GHL L+K P+H+T S H V SLR GSR+V VV
Sbjct: 27 KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVV 86
Query: 61 SSPS-AVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFST 120
SS A +ECF KND+VLANRP + GK + YN+T M APYG+HWRNLRR+ T+EIFST
Sbjct: 87 SSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFST 146
Query: 121 TRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY---- 180
RLN FL +R DEVR L+ +L + + T VEL+PML DLTFN +MRM+ GK+YY
Sbjct: 147 HRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEET 206
Query: 181 -EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELID 240
+E+++K+ R++V V A+ + N D++PI + +E R+ KLG+ +D+ LQ LID
Sbjct: 207 TDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKKLGEETDKFLQGLID 266
Query: 241 EIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLN 300
+ R Q+ TMIDHLL LQK++ EYY+DQIIKG+I ++++AG +T+AVTLEWALS LLN
Sbjct: 267 DKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLN 326
Query: 301 NPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCK 360
+P+V+ KA+ EID VG +RL+ EADLS L YL+ I+ ETLRL+PA PLL+PH AS DCK
Sbjct: 327 HPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCK 386
Query: 361 IGGYDVPRDTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPG 420
IG YD+PR T +L+N WAIHRDPN W+D +FKPER ++ KLL FG GRR+CPG
Sbjct: 387 IGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKE--EEAQKLLAFGLGRRACPG 446
Query: 421 VGMAQRVVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILN 470
G+AQR+VGL L SLIQ FEWER+ N VDM EG G T+PKA L A CK RP + I++
Sbjct: 447 SGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502
BLAST of MS009088 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 515.4 bits (1326), Expect = 6.9e-145
Identity = 257/473 (54.33%), Postives = 340/473 (71.88%), Query Frame = 0
Query: 3 RRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQ 62
R +NLPP P ++PI+G+LH +KHP+H+TF LS YG +FSL FGSR+VVVVSSPS
Sbjct: 26 RLKNLPPGPP-TIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAH 85
Query: 63 ECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLG 122
ECFTKNDI+LANRP +GK + YN+TT+ A YG+HWRNLRRI T+++ S RLN FLG
Sbjct: 86 ECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFLG 145
Query: 123 IREDEVRNLMLKLCENSFEE---FTAVELEPMLFDLTFNIVMRMVAGKKYY-------EE 182
+R DE L+ KL ++ E FT VEL P L ++TFN +MRM++GK+YY +
Sbjct: 146 VRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDV 205
Query: 183 QQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIR 242
+++K+FRE+++++++ +GA N GDF+P+ +D EKR ++ +RS+ L+ LI+E R
Sbjct: 206 EEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAKRSNAFLEGLIEEHR 265
Query: 243 --NQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNN 302
N TMIDHLL L +++PEYYSD +IKGLIQ +LLAG DT+AVT+EW +S LLN+
Sbjct: 266 RGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLNH 325
Query: 303 PEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKI 362
PEVL+KAK E+D +G+ +LV+E DLS L YLQ II ETLRL+P APLL+PH +S DC I
Sbjct: 326 PEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCTI 385
Query: 363 GGYDVPRDTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGV 422
G ++VP+DTIIL NVW IHRDP W DA +FKPER V+ K++ FG GRR+CPG+
Sbjct: 386 GEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEEEVN--KVMAFGLGRRACPGL 445
Query: 423 GMAQRVVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPV 464
+AQR VG T+ LIQ FEWER S +DM EGKG+TMP L A CK P+
Sbjct: 446 SLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMKIPLRAMCKALPI 495
BLAST of MS009088 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 508.8 bits (1309), Expect = 6.5e-143
Identity = 253/478 (52.93%), Postives = 340/478 (71.13%), Query Frame = 0
Query: 1 RSRR-QNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPS 60
R+R+ +NLPP P SLPI+G+LH +K P+H+TF LS KYGHVFSL FGSR+VVVVSS S
Sbjct: 25 RARKFKNLPPGPP-SLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSAS 84
Query: 61 AVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNM 120
Q+CFTKND+VLANRP SGK + YN+TT+ YGEHWRNLRRI L++ S R+N
Sbjct: 85 EFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINS 144
Query: 121 FLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEE------- 180
F GIR DE + L+ +L ++S F +EL L+D+TFN +MRM++GK+YY E
Sbjct: 145 FSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDL 204
Query: 181 QQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIR 240
Q++ +FR+MV+++L GA N DF+P+ ++D EKR+ + ++D L+ LI+E R
Sbjct: 205 QEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHR 264
Query: 241 NQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPE 300
++ NTMIDHLL LQ ++PEYY+DQIIKGL +LLAG D++AVTLEW++S LLN+PE
Sbjct: 265 TKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPE 324
Query: 301 VLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGG 360
VL+K K E+D VGQ+RLV+E+DL L YL+ +I ETLRL APLL+PH S +C IGG
Sbjct: 325 VLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGG 384
Query: 361 YDVPRDTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGM 420
Y VP+DTI+LIN WAIHRDP LW +AT FKPER ++ KL+ FG GRR+CPG G+
Sbjct: 385 YKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGELE--KLIAFGMGRRACPGEGL 444
Query: 421 AQRVVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNE 471
A R + +TLA LIQ F+W+ ++ +D++E G T+ K L A CK RPV+ + +
Sbjct: 445 AIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLKAMCKSRPVINKVFKQ 499
BLAST of MS009088 vs. ExPASy TrEMBL
Match:
A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)
HSP 1 Score: 961.4 bits (2484), Expect = 1.3e-276
Identity = 470/473 (99.37%), Postives = 473/473 (100.00%), Query Frame = 0
Query: 1 RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSA 60
RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSA
Sbjct: 24 RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSA 83
Query: 61 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 120
VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF
Sbjct: 84 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 143
Query: 121 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFRE 180
LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFRE
Sbjct: 144 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFRE 203
Query: 181 MVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNT 240
MVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNT
Sbjct: 204 MVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNT 263
Query: 241 MIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAE 300
MIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAE
Sbjct: 264 MIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAE 323
Query: 301 IDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTI 360
IDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTI
Sbjct: 324 IDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTI 383
Query: 361 ILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLT 420
ILIN+WAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLT
Sbjct: 384 ILINIWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLT 443
Query: 421 LASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEAII 474
LASLIQGFEWERMSNSLVDM+EGKGLTMPKAQHLVAKCKPRP+MKLILNEAII
Sbjct: 444 LASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAKCKPRPIMKLILNEAII 496
BLAST of MS009088 vs. ExPASy TrEMBL
Match:
A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)
HSP 1 Score: 774.2 bits (1998), Expect = 3.0e-220
Identity = 370/474 (78.06%), Postives = 427/474 (90.08%), Query Frame = 0
Query: 1 RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSA 60
R R+NLPPSP F LP++GHLHLVKHPVH+T H+LSH YGHVFSLR+GSR+VVVVSSPSA
Sbjct: 104 RPYRRNLPPSPFFRLPLIGHLHLVKHPVHRTLHKLSHNYGHVFSLRYGSRLVVVVSSPSA 163
Query: 61 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 120
VQECFTKNDIVLANRPLLKSGK LAYNHTT+AV+PYGEHWRNLRRI TLEIFST+RLN+F
Sbjct: 164 VQECFTKNDIVLANRPLLKSGKFLAYNHTTLAVSPYGEHWRNLRRISTLEIFSTSRLNLF 223
Query: 121 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY-----EEQQS 180
LGIREDEV+NL+ KL +S +EF AVEL+ ML D TFNIV+RM+AGKKYY +E QS
Sbjct: 224 LGIREDEVKNLLRKLRGDSLQEFRAVELDSMLLDHTFNIVVRMIAGKKYYGEDLRDEGQS 283
Query: 181 KKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQE 240
KKFREMV ++LA+ G N GDF+P+WNWIDPTG+EKRI+K+G+RSDELLQEL+DEIRNQ+
Sbjct: 284 KKFREMVKEILANGGTANLGDFVPLWNWIDPTGYEKRIMKIGERSDELLQELVDEIRNQK 343
Query: 241 GGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLE 300
G NTMIDHLL LQ +PEY+SDQIIKGL Q++LLAGIDTAA+TLEWALS+LLNNPEVL+
Sbjct: 344 DGGNTMIDHLLSLQNTQPEYFSDQIIKGLAQILLLAGIDTAAITLEWALSQLLNNPEVLK 403
Query: 301 KAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDV 360
KA+AEID F+G++RLV+E DLSSL YLQG+I ETLRLNPAAPLL+PHCAS DCKI GYD+
Sbjct: 404 KARAEIDHFIGEDRLVSEGDLSSLSYLQGVITETLRLNPAAPLLVPHCASEDCKIEGYDI 463
Query: 361 PRDTIILINVWAIHRDPNLWEDATNFKPERHGNTI-GVDSYKLLPFGSGRRSCPGVGMAQ 420
PRDTIILIN WAIHRD N+WEDAT+FKPERH NT+ GVDSYKLLPFG GRR+CPG+GMAQ
Sbjct: 464 PRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRACPGMGMAQ 523
Query: 421 RVVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLIL 469
RVVGLTLASLIQ FEWER+++SLVDM+EG+GLTMPKAQ LVAKCKPRP++K IL
Sbjct: 524 RVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKAIL 577
BLAST of MS009088 vs. ExPASy TrEMBL
Match:
A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)
HSP 1 Score: 761.1 bits (1964), Expect = 2.7e-216
Identity = 360/469 (76.76%), Postives = 416/469 (88.70%), Query Frame = 0
Query: 4 RQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQE 63
R NLPP+PLFSLP++GH HL+KHP+H++ H LS K+GHV SLRFGSR+VVV+SSPSAVQE
Sbjct: 27 RLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQKHGHVISLRFGSRLVVVISSPSAVQE 86
Query: 64 CFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLGI 123
CFTKNDI+LANRPLL +GK LAYNHTT+AV+PYGEHWRNLRRI +LEIFST RLNMF+GI
Sbjct: 87 CFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYGEHWRNLRRICSLEIFSTNRLNMFVGI 146
Query: 124 REDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFREMVT 183
RE+EV+ L+ KLC + EEF VELEPML DLTFN+VM MV G+++Y+E SKKFR++VT
Sbjct: 147 RENEVKRLLRKLCGDWSEEFREVELEPMLSDLTFNVVMGMVGGRRFYDEGYSKKFRDLVT 206
Query: 184 QVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMID 243
Q++AH G++NPGDFIP+WNW+DPTGF+KRI+ L +R DELLQELIDE R+ + G NTMI
Sbjct: 207 QIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDELLQELIDEARDGKDGGNTMIH 266
Query: 244 HLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDC 303
HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+AVTLEWALS LLNNP+VL+KA+AEID
Sbjct: 267 HLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSAVTLEWALSHLLNNPDVLKKARAEIDD 326
Query: 304 FVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILI 363
+G+ERLVNE+DL +L YLQGII ETLRLNPAAPLL+PHCA DCKI GYDVPRDTI+LI
Sbjct: 327 LIGKERLVNESDLPNLKYLQGIISETLRLNPAAPLLVPHCAFQDCKIEGYDVPRDTIVLI 386
Query: 364 NVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLAS 423
N WAIHRDPNLWED T FKPERHG+T G DSYKLLPFG GRR+CPGVGMAQRVVGL LAS
Sbjct: 387 NTWAIHRDPNLWEDPTAFKPERHGDTSGADSYKLLPFGLGRRACPGVGMAQRVVGLALAS 446
Query: 424 LIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEAI 473
LIQGFEWER+S+SLVDMSEG+GLTMPKAQ LVAKCKPRP+MK I+N I
Sbjct: 447 LIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAKCKPRPIMKAIMNVEI 495
BLAST of MS009088 vs. ExPASy TrEMBL
Match:
A0A6J1L2W8 (cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1)
HSP 1 Score: 753.8 bits (1945), Expect = 4.3e-214
Identity = 353/469 (75.27%), Postives = 417/469 (88.91%), Query Frame = 0
Query: 4 RQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQE 63
R NLPP+PLFSLP++GH HL+KHP+H++ H LS K+GHV SLRFGSR+VV++SSPSAVQE
Sbjct: 27 RLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQKHGHVISLRFGSRLVVLISSPSAVQE 86
Query: 64 CFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLGI 123
CFTKNDI+LANRPLL +GK LAYNHTT+A++PYGEHWRNLRRI +LEIFST+RLN+FLGI
Sbjct: 87 CFTKNDIILANRPLLDTGKYLAYNHTTLAISPYGEHWRNLRRICSLEIFSTSRLNLFLGI 146
Query: 124 REDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQQSKKFREMVT 183
R++EV+ L+ KLC + EEF VE+EPML DLTFN+VM MV G+++Y+E SKKFR++VT
Sbjct: 147 RQNEVKRLLRKLCGDWLEEFREVEVEPMLSDLTFNVVMGMVGGRRFYDEGYSKKFRDLVT 206
Query: 184 QVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGGDNTMID 243
Q++AH G++NPGDFIP+WNW+DPTGF+KRI+ L +R DELLQELIDE R+ + G N+MI
Sbjct: 207 QIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNLARRGDELLQELIDEARDGKDGGNSMIH 266
Query: 244 HLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKAKAEIDC 303
HLLCLQK+EPEYYSD IIKGLIQVI LAGIDT+AVTLEWALS LLNNP+VL+KA+AEID
Sbjct: 267 HLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTSAVTLEWALSHLLNNPDVLKKARAEIDD 326
Query: 304 FVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPRDTIILI 363
+G+ERLVNE+DL +L YLQGII ETLRLNPAAPLL+PHCA DCKI GYDVPRDTI+LI
Sbjct: 327 LIGKERLVNESDLPNLRYLQGIISETLRLNPAAPLLVPHCAFQDCKIEGYDVPRDTIVLI 386
Query: 364 NVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVVGLTLAS 423
N WAIHRDP+LWED T FKPERHG++ G DSYKLLPFG GRR+CPGVGMAQRVVGL LAS
Sbjct: 387 NAWAIHRDPDLWEDPTAFKPERHGDSSGADSYKLLPFGLGRRACPGVGMAQRVVGLALAS 446
Query: 424 LIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEAI 473
LIQGFEWER+S+SLVDMSEG+GLTMPKAQ LVAKCKPRP+MK I+N I
Sbjct: 447 LIQGFEWERVSSSLVDMSEGQGLTMPKAQPLVAKCKPRPIMKAIMNVEI 495
BLAST of MS009088 vs. ExPASy TrEMBL
Match:
A0A6J1D8L6 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017928 PE=3 SV=1)
HSP 1 Score: 749.6 bits (1934), Expect = 8.0e-213
Identity = 361/473 (76.32%), Postives = 408/473 (86.26%), Query Frame = 0
Query: 3 RRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGHVFSLRFGSRVVVVVSSPSAVQ 62
RR NLPPSPLFSLPI+GHLHLVKH VH+T H LSH YG VFSLRFGSR+VVVVSSPS VQ
Sbjct: 23 RRNNLPPSPLFSLPIIGHLHLVKHSVHRTLHNLSHNYGRVFSLRFGSRLVVVVSSPSVVQ 82
Query: 63 ECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMFLG 122
ECFTKNDI+LANRPLL + K LAYN TTMAVAPYG+HWR+LRRIG LEIFSTTR+N+FLG
Sbjct: 83 ECFTKNDIILANRPLLDTAKHLAYNQTTMAVAPYGDHWRHLRRIGALEIFSTTRMNLFLG 142
Query: 123 IREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYYEEQ-----QSKK 182
IRE EV+ LM KLC +SF EFT VE M+ DL FNI+MR+VAGKKYY++ +S+K
Sbjct: 143 IREGEVKRLMRKLCGSSFVEFTVVEPGSMILDLMFNIIMRIVAGKKYYDDDMSNDGKSRK 202
Query: 183 FREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQEGG 242
FRE V + +AH GATNPGDFIPIWNWIDPTG EK+++KLG DELLQEL+DEIRNQ+
Sbjct: 203 FREAVKETMAHGGATNPGDFIPIWNWIDPTGLEKKMMKLGHTMDELLQELLDEIRNQKNE 262
Query: 243 DNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLEKA 302
NTMI+HLLCLQK+EPE +SD IIKGL+ VIL+AGIDT VTLEWALS LLNNPEVLEKA
Sbjct: 263 GNTMIEHLLCLQKSEPENHSDLIIKGLVHVILIAGIDTIGVTLEWALSHLLNNPEVLEKA 322
Query: 303 KAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDVPR 362
KAEID +GQER+VNEADLSSL Y+QGII+ETLRL PAAPLL+ HC S DCKIGGYD+PR
Sbjct: 323 KAEIDNSIGQERMVNEADLSSLSYVQGIIFETLRLTPAAPLLVAHCPSEDCKIGGYDIPR 382
Query: 363 DTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQRVV 422
D I+LIN WAIHRDP LWEDAT+F+PERH N IGVDSYKLLPFG GRR+CPG+GMAQRVV
Sbjct: 383 DAIVLINAWAIHRDPELWEDATSFRPERHTNAIGVDSYKLLPFGLGRRACPGMGMAQRVV 442
Query: 423 GLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNE 471
GLTLASLIQ FEW+R S+ LVDM+EG GLTMPKAQ LVAKC+PRP+M+ ILNE
Sbjct: 443 GLTLASLIQCFEWKRASSLLVDMTEGGGLTMPKAQPLVAKCRPRPIMRAILNE 495
BLAST of MS009088 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 525.8 bits (1353), Expect = 3.6e-149
Identity = 259/477 (54.30%), Postives = 352/477 (73.79%), Query Frame = 0
Query: 3 RRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH--VFSLRFGSRVVVVVSSPSA 62
R+ NLPPSP +SLP++GHL L+K P+H+TF LS + +FSLR G+R+V V SS S
Sbjct: 26 RKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSI 85
Query: 63 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 122
+ECFTKND+VLANRP K +AY++TTM A YG+HWRNLRRIG++EIFS RLN F
Sbjct: 86 AEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSF 145
Query: 123 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY-----EEQQS 182
L IR+DE+R L+ +L N +EF V+++ ML DLTFN ++RMVAGK+YY ++ ++
Sbjct: 146 LSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEA 205
Query: 183 KKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQE 242
K+ R+++ V+A GA N D++P+ + + +E R+ KL R DE LQ L+DE R +
Sbjct: 206 KRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKLAGRLDEFLQGLVDEKREAK 265
Query: 243 GGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVLE 302
NTMIDHLL LQ+++P+Y++D+IIKG + ++LAG DT+AVTLEWALS +LN+P+VL
Sbjct: 266 EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLN 325
Query: 303 KAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYDV 362
KA+ EID +G +RL++E+D+S+L YLQ I+ ETLRL PAAP+L+PH AS DCK+ GYD+
Sbjct: 326 KARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDM 385
Query: 363 PRDTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQR 422
PR TI+L NVWAIHRDP LW+D +FKPER ++ KL+PFG GRR+CPG G+A R
Sbjct: 386 PRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKE--GEAQKLMPFGLGRRACPGSGLAHR 445
Query: 423 VVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEAI 473
++ LTL SLIQ EWE++ VDMSEGKG+TMPKA+ L A C+ RP + I NE++
Sbjct: 446 LINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMCRARPSVVKIFNESV 497
BLAST of MS009088 vs. TAIR 10
Match:
AT5G36220.1 (cytochrome p450 81d1 )
HSP 1 Score: 516.5 bits (1329), Expect = 2.2e-146
Identity = 264/480 (55.00%), Postives = 343/480 (71.46%), Query Frame = 0
Query: 1 RSRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH-----VFSLRFGSRVVVVV 60
+ ++QNLPPSP LPI+GHL L+K P+H+T S H V SLR GSR+V VV
Sbjct: 27 KPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVV 86
Query: 61 SSPS-AVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFST 120
SS A +ECF KND+VLANRP + GK + YN+T M APYG+HWRNLRR+ T+EIFST
Sbjct: 87 SSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFST 146
Query: 121 TRLNMFLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY---- 180
RLN FL +R DEVR L+ +L + + T VEL+PML DLTFN +MRM+ GK+YY
Sbjct: 147 HRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEET 206
Query: 181 -EEQQSKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELID 240
+E+++K+ R++V V A+ + N D++PI + +E R+ KLG+ +D+ LQ LID
Sbjct: 207 TDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--SSYENRVKKLGEETDKFLQGLID 266
Query: 241 EIRNQEGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLN 300
+ R Q+ TMIDHLL LQK++ EYY+DQIIKG+I ++++AG +T+AVTLEWALS LLN
Sbjct: 267 DKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLN 326
Query: 301 NPEVLEKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCK 360
+P+V+ KA+ EID VG +RL+ EADLS L YL+ I+ ETLRL+PA PLL+PH AS DCK
Sbjct: 327 HPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCK 386
Query: 361 IGGYDVPRDTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPG 420
IG YD+PR T +L+N WAIHRDPN W+D +FKPER ++ KLL FG GRR+CPG
Sbjct: 387 IGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKE--EEAQKLLAFGLGRRACPG 446
Query: 421 VGMAQRVVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILN 470
G+AQR+VGL L SLIQ FEWER+ N VDM EG G T+PKA L A CK RP + I++
Sbjct: 447 SGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502
BLAST of MS009088 vs. TAIR 10
Match:
AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )
HSP 1 Score: 513.8 bits (1322), Expect = 1.4e-145
Identity = 263/478 (55.02%), Postives = 340/478 (71.13%), Query Frame = 0
Query: 3 RRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH--VFSLRFGSRVVVVVSSPSA 62
RR NLPPSP ++LP++GHL L+K P+H+ F +S G + SLR G+R+V VVSS S
Sbjct: 26 RRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAPIISLRLGNRLVFVVSSHSL 85
Query: 63 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 122
+ECFTKND+VLANR + K ++Y TT+ A YG+HWRNLRRIG +EIFS RLN F
Sbjct: 86 AEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRNLRRIGAVEIFSAHRLNSF 145
Query: 123 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY-----EEQQS 182
IR DE+ L+ L NS EFT VE++ M +LTFN ++RM+AGK YY ++ ++
Sbjct: 146 SSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIRMLAGKCYYGDGAEDDPEA 205
Query: 183 KKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIR-NQ 242
K+ RE++ + + GA N D++PI WI TG EKRI K+ R DE LQ L+DE R +
Sbjct: 206 KRVRELIAEGMGCFGAGNTADYLPILTWI--TGSEKRIKKIASRLDEFLQGLVDERREGK 265
Query: 243 EGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVL 302
E NTM+DHLLCLQ+ +PEYY+D IIKG++ ++LAG DT+AVTLEW LS LLN+P++L
Sbjct: 266 EKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSAVTLEWTLSALLNHPQIL 325
Query: 303 EKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYD 362
KA+ EID VG RLV E+DLS L YLQ I+ E+LRL PA+PLL+PH AS DCK+GGY
Sbjct: 326 SKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASPLLVPHVASEDCKVGGYH 385
Query: 363 VPRDTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQ 422
+PR T++L N WAIHRDP +W+D T+FKPER ++ KLL FG GRR+CPG G+AQ
Sbjct: 386 MPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKE--GEAQKLLGFGLGRRACPGSGLAQ 445
Query: 423 RVVGLTLASLIQGFEWERMSNSLVDMSE-GKGLTMPKAQHLVAKCKPRPVMKLILNEA 472
R+ LT+ SLIQ FEWER+ VDM+E G G+ MPKA LVA CK RPV+ ILNE+
Sbjct: 446 RLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVAMCKARPVVGKILNES 499
BLAST of MS009088 vs. TAIR 10
Match:
AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )
HSP 1 Score: 511.5 bits (1316), Expect = 7.1e-145
Identity = 246/470 (52.34%), Postives = 336/470 (71.49%), Query Frame = 0
Query: 2 SRRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH--VFSLRFGSRVVVVVSSPS 61
+R+ NLPPSP + LP++GHLHL+K PVH+TFH +S G+ +F LR G+R+V V+SS S
Sbjct: 26 NRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGNAPIFHLRLGNRLVYVISSHS 85
Query: 62 AVQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNM 121
+ECFTKND+VLANRP + K + YN T M A YG+HWRNLRRI +EIFS+ R++
Sbjct: 86 IAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDHWRNLRRIAAVEIFSSHRIST 145
Query: 122 FLGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY-----EEQQ 181
F IR+DE+R L+ L +S F VEL+ +L +L FN ++ MVAGK+YY + +
Sbjct: 146 FSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNNIIMMVAGKRYYGTGTEDNDE 205
Query: 182 SKKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIRNQ 241
+K RE++ +++A G+ N D++P NW+ T FE + LG R D +LQ+L+DE R +
Sbjct: 206 AKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTKILGNRLDRVLQKLVDEKRAE 265
Query: 242 EGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVL 301
+ T+IDHLL Q+ EPEYY+D IIKG+I ++LAG DT++VTLEWA+S LLN+PE+L
Sbjct: 266 KEKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVLAGTDTSSVTLEWAMSNLLNHPEIL 325
Query: 302 EKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYD 361
EKA+AEID +G +RLV E+D+ +L YLQ I+ ETLRL PA PLL+PH +S +CK+ GYD
Sbjct: 326 EKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYD 385
Query: 362 VPRDTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQ 421
+PR T++L NVWA+HRDP LWE+ FKPER ++ KL+PFG GRR+CPG + +
Sbjct: 386 MPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKE--GEARKLMPFGMGRRACPGAELGK 445
Query: 422 RVVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVM 465
R+V L L LIQ FEWER+ LVDM+EG+G+TMPKA L A CK R ++
Sbjct: 446 RLVSLALGCLIQSFEWERVGAELVDMTEGEGITMPKATPLRAMCKARAIV 491
BLAST of MS009088 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 510.0 bits (1312), Expect = 2.1e-144
Identity = 258/477 (54.09%), Postives = 338/477 (70.86%), Query Frame = 0
Query: 3 RRQNLPPSPLFSLPIVGHLHLVKHPVHQTFHELSHKYGH--VFSLRFGSRVVVVVSSPSA 62
R+ NLPPSP ++LP++GHL L+K P+H+ F +S G + SLR G+R++ VVSS S
Sbjct: 26 RKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAPIISLRLGNRLLFVVSSHSI 85
Query: 63 VQECFTKNDIVLANRPLLKSGKLLAYNHTTMAVAPYGEHWRNLRRIGTLEIFSTTRLNMF 122
+ECFTKND++LANR S K ++Y ++T+ A Y EHWRNLRRIG LEIFS RLN F
Sbjct: 86 AEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRNLRRIGALEIFSAHRLNSF 145
Query: 123 LGIREDEVRNLMLKLCENSFEEFTAVELEPMLFDLTFNIVMRMVAGKKYY-----EEQQS 182
IR DE+R L+ +L NS FT VE++ M DLTFN ++RM+AGK YY ++ ++
Sbjct: 146 SSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIRMLAGKCYYGDGKEDDPEA 205
Query: 183 KKFREMVTQVLAHVGATNPGDFIPIWNWIDPTGFEKRIIKLGQRSDELLQELIDEIR-NQ 242
K+ R ++ + ++ G N D+IPI WI T E RI KL R DE LQ L+DE R +
Sbjct: 206 KRVRTLIAEAMSSSGPGNAADYIPILTWI--TYSETRIKKLAGRLDEFLQGLVDEKREGK 265
Query: 243 EGGDNTMIDHLLCLQKNEPEYYSDQIIKGLIQVILLAGIDTAAVTLEWALSRLLNNPEVL 302
E +NTM+DHLLCLQ+ +PEYY D+IIKG + ++ G DT AVTLEWALS LLNNPEVL
Sbjct: 266 EKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVL 325
Query: 303 EKAKAEIDCFVGQERLVNEADLSSLIYLQGIIWETLRLNPAAPLLIPHCASADCKIGGYD 362
KA+ EID +G +RL+ E+D+ +L YLQ I+ ETLRL PAAP+L+PH AS DCK+GGYD
Sbjct: 326 NKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYD 385
Query: 363 VPRDTIILINVWAIHRDPNLWEDATNFKPERHGNTIGVDSYKLLPFGSGRRSCPGVGMAQ 422
+PR T++L N WAIHRDP LW+D T+FKPER ++ KL+PFG GRR+CPG G+AQ
Sbjct: 386 MPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKE--GEAKKLMPFGLGRRACPGSGLAQ 445
Query: 423 RVVGLTLASLIQGFEWERMSNSLVDMSEGKGLTMPKAQHLVAKCKPRPVMKLILNEA 472
R+V L+L SLIQ FEWER+ VDM+EG GLTMPKA+ L A C+ R + IL ++
Sbjct: 446 RLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGKILPDS 498
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| W8JMU7 | 2.4e-161 | 57.42 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
| Q6WNQ8 | 5.6e-147 | 54.58 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
| Q9FG65 | 3.1e-145 | 55.00 | Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1 | [more] |
| Q6WNQ9 | 6.9e-145 | 54.33 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
| P93147 | 6.5e-143 | 52.93 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1D5S1 | 1.3e-276 | 99.37 | cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV... | [more] |
| A0A6J1D5S6 | 3.0e-220 | 78.06 | isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE... | [more] |
| A0A6J1G5P7 | 2.7e-216 | 76.76 | cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1 | [more] |
| A0A6J1L2W8 | 4.3e-214 | 75.27 | cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1 | [more] |
| A0A6J1D8L6 | 8.0e-213 | 76.32 | cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017928 PE=3 SV... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G37370.1 | 3.6e-149 | 54.30 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
| AT5G36220.1 | 2.2e-146 | 55.00 | cytochrome p450 81d1 | [more] |
| AT4G37340.1 | 1.4e-145 | 55.02 | cytochrome P450, family 81, subfamily D, polypeptide 3 | [more] |
| AT4G37320.1 | 7.1e-145 | 52.34 | cytochrome P450, family 81, subfamily D, polypeptide 5 | [more] |
| AT4G37360.1 | 2.1e-144 | 54.09 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
