MS009066 (gene) Bitter gourd (TR) v1

Overview
NameMS009066
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRNA polymerase sigma factor sigA
Locationscaffold687: 90359 .. 93061 (-)
RNA-Seq ExpressionMS009066
SyntenyMS009066
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGCAACAGCTGCAATAATTGGGCTTGGTGCAGCAAAGAGGCTGTTGAATTCTTCATCCTATTATTCTGATTTCCCAGAAAAGCTTTTATATGCCAATGACCATAGGTTGGGGCAAAGCCAAGTTTCCCCCACAAAGAGTGTGGTAATAGCGAAAAGTTCGGCTAACTTTTCCCCAAGCTTCCCATCGTCGAATCGACACACACAATGCATCAAAGCTGTGAAGGAACATGTAGATACTCCCTCTTCCCCAACTGTTGAGCCATGGCTTCAGAACTCCACACAATGGGAAGATGAAGAAATTGATCTCAAATGTACTGTGGAGGCTCTTCTTTTGCTGCAGAAGTCCATGCTGGAGAAGCAATGGAATCTATCTTTTGAGCAAACTGTGCCGACTGATGCAAGTAGAGGAAGAACTCAGAAGAAAGTACCTGTTACTTGTTCGGGTGTATCGGCCCGGCAAAGGAGAATGAGCTCTAAGAGGAAGATACAGAGTAAAAACTGTTTTATGGTTCAACCAAAAATCAGTAAGCAGCTAAGATCTACCATGAGTCCTGAGCTGCTCCAAACCCGTTTGAAGGGCTATGTGAAAGGTTTAGTGAGTGAAGAGCTACTTTCTCATGCTGAAGTCGTACGTCTTTCGAAGAAAATTAAAGTCGGTCTCGCGTTGGAGGAGCGTAAAACCAGGTTACCACATGCTTCTTGCTCAGATTCTCATAGATTACTTTAGCTGTGATTTACAATTGAGAAGTAGCAGATAGTATCATTCCTGCATGCTGTGAGAAAGCAGATTATCATATCTTTATGCAGTCTTATTTCTCCATTCATTGTCAAAACTGCTGCACTAATAATGATATCATGAACTTCATATTGTACATTTTTTCCCCTAACATCTGTAATGACTACCAGACTGAAGGAAAGACTAGGATGTGAGCCCTCTGAAGACCAACTGGCAATATCGCTTAAGATTTCTCGCACAGAGTTACGTTCGAGAGTAATTGAGTGTTCATTGGCGAAGGAGAAGCTAGCAATGAGCAACGTTCGTTTGGTAATGTCGATTGCACAAAGATATGACAATATGGGGGCTGAAATGGCTGACTTAGTTCAGGTGAAGAGCACATGGCAGTCCATGCAGCAAACTCTACAACTTATTAGCCTCTCCCCCTAATATGAAGAGTTTGTTTATGCTTAAATGTAGGGTGGCTTGATTGGACTTCTCCGCGGGATCGAAAAGTTTGATTCCTCAAAGGGGTTCAGAATCTCAACCTATGTTTATTGGTGGATACGCCAGGTGAGGATATAAATTCTTTTGAACAAATTGATATCCCAAGTTTAGTTCCTAAACTTTTAGAGTTGTGTCAATAAGTCTTAAACATTCAATTTTGTGTTTGATAAATCCTTGAAGTTTAAAAGTGTCGAATAAAATTCCTGAGCTTTCGATTTTGCTTCTAATAGGTCTTGATATTTTTCAAAGTTCAAGGATTTATTAGACACAAAATTGAAAGTTTAGAGTTCTACTAAAGGAGATTTGGTTCAAATTATTTTTAGCGCAAGCACCTGAACTCGTGCACTGAGTAGTTGATTGTGTTCCTCCATGGACAGGGTGTTTCAAGAGCATTAGTTGAGAATTCAAGAACGCTGAGATTGCCAACTCATTTGCACGAGAGATTAGGATTAATTCGCAATGCCAAAGTTAGACTGCAAGAGAAGGGAATCACTCCATCACTTGATGTAAGTGGATAACTGCCATAACACTACAGCTAGAGCAAAATATTGTTGAACTTGAAGAAAAAACAAGTTCAACTGCAAATTTAGATGAGCTTTGTGATTGCTTTGACACTTATCATCTATTCATCTCAGAGGATTGCAGAATCCCTCAACATGTCCAAAAAGAAAGTCAAGAATGCCACTGAGGTATAAAAAATTTTTAATAACCAATTTGCTCTCTTCTTTCCTAATAAGAAGCTAACCTCTCATATCTTTTAGGCAATCAGCAAGGTGTTCTCTTTAGACCGTGAAGCATTCCCTTCATTGAATGGTCTTCCTGGCGAAACTCATCATAGTGTAAGCCTTAATAGCAACTCCCTTTCTGCAAACTTCACAGAATTTTCAGAACCTTATTTACTTAAATTCTTGGCACTCTCCTTGAGTTTTTGTTGTTAACATTTTGTTTTTGCAGTACATTGCGGATAATCGCCTTGAGAACGACCCGTGGCATGGCACAGACACGTGGATTCTGAAGGTAAATTGATTGATAGCCAGACCAAAAAAACAGACTTAGAGAATGAAAGATTATACCATTTTTATCGATATACAAGATAATGAAGATGATGAACGAAAAGTTTGTTTGCTTACCTAGCTGAATGATCAATTAATCCACTCGAAAATATTGACAGGCTGAGGTAAACAAACTCATCAATATGACGCTTTGCGACCGAGAAAGAGAGATCATTCGTCTTTACCACGGTCTGGATAACGAATGTCTGACATGGGAGGAAATAAGCAAACGGTAAGTCAAAGGAAATTATCTGAGCAAAATGTAAATGACATTCTAAGTAAGCTGACAAAATGCAGTGTTTGCTCTGAGATTTTCAGCATAGGGTTGTCCAGAGAAAGGGTAAGACAAGTTGGACTGGTGGCATTGGAGAAACTAAAGAAGGCAGCCAAGACAAGGAAGATGGAAGCCATGCTGCTCAAACAT

mRNA sequence

ATGATGGCAACAGCTGCAATAATTGGGCTTGGTGCAGCAAAGAGGCTGTTGAATTCTTCATCCTATTATTCTGATTTCCCAGAAAAGCTTTTATATGCCAATGACCATAGGTTGGGGCAAAGCCAAGTTTCCCCCACAAAGAGTGTGGTAATAGCGAAAAGTTCGGCTAACTTTTCCCCAAGCTTCCCATCGTCGAATCGACACACACAATGCATCAAAGCTGTGAAGGAACATGTAGATACTCCCTCTTCCCCAACTGTTGAGCCATGGCTTCAGAACTCCACACAATGGGAAGATGAAGAAATTGATCTCAAATGTACTGTGGAGGCTCTTCTTTTGCTGCAGAAGTCCATGCTGGAGAAGCAATGGAATCTATCTTTTGAGCAAACTGTGCCGACTGATGCAAGTAGAGGAAGAACTCAGAAGAAAGTACCTGTTACTTGTTCGGGTGTATCGGCCCGGCAAAGGAGAATGAGCTCTAAGAGGAAGATACAGAGTAAAAACTGTTTTATGGTTCAACCAAAAATCAGTAAGCAGCTAAGATCTACCATGAGTCCTGAGCTGCTCCAAACCCGTTTGAAGGGCTATGTGAAAGGTTTAGTGAGTGAAGAGCTACTTTCTCATGCTGAAGTCGTACGTCTTTCGAAGAAAATTAAAGTCGGTCTCGCGTTGGAGGAGCGTAAAACCAGACTGAAGGAAAGACTAGGATGTGAGCCCTCTGAAGACCAACTGGCAATATCGCTTAAGATTTCTCGCACAGAGTTACGTTCGAGAGTAATTGAGTGTTCATTGGCGAAGGAGAAGCTAGCAATGAGCAACGTTCGTTTGGTAATGTCGATTGCACAAAGATATGACAATATGGGGGCTGAAATGGCTGACTTAGTTCAGGGTGGCTTGATTGGACTTCTCCGCGGGATCGAAAAGTTTGATTCCTCAAAGGGGTTCAGAATCTCAACCTATGTTTATTGGTGGATACGCCAGGGTGTTTCAAGAGCATTAGTTGAGAATTCAAGAACGCTGAGATTGCCAACTCATTTGCACGAGAGATTAGGATTAATTCGCAATGCCAAAGTTAGACTGCAAGAGAAGGGAATCACTCCATCACTTGATAGGATTGCAGAATCCCTCAACATGTCCAAAAAGAAAGTCAAGAATGCCACTGAGGCAATCAGCAAGGTGTTCTCTTTAGACCGTGAAGCATTCCCTTCATTGAATGGTCTTCCTGGCGAAACTCATCATAGTTACATTGCGGATAATCGCCTTGAGAACGACCCGTGGCATGGCACAGACACGTGGATTCTGAAGGCTGAGGTAAACAAACTCATCAATATGACGCTTTGCGACCGAGAAAGAGAGATCATTCGTCTTTACCACGGTCTGGATAACGAATGTCTGACATGGGAGGAAATAAGCAAACGCATAGGGTTGTCCAGAGAAAGGGTAAGACAAGTTGGACTGGTGGCATTGGAGAAACTAAAGAAGGCAGCCAAGACAAGGAAGATGGAAGCCATGCTGCTCAAACAT

Coding sequence (CDS)

ATGATGGCAACAGCTGCAATAATTGGGCTTGGTGCAGCAAAGAGGCTGTTGAATTCTTCATCCTATTATTCTGATTTCCCAGAAAAGCTTTTATATGCCAATGACCATAGGTTGGGGCAAAGCCAAGTTTCCCCCACAAAGAGTGTGGTAATAGCGAAAAGTTCGGCTAACTTTTCCCCAAGCTTCCCATCGTCGAATCGACACACACAATGCATCAAAGCTGTGAAGGAACATGTAGATACTCCCTCTTCCCCAACTGTTGAGCCATGGCTTCAGAACTCCACACAATGGGAAGATGAAGAAATTGATCTCAAATGTACTGTGGAGGCTCTTCTTTTGCTGCAGAAGTCCATGCTGGAGAAGCAATGGAATCTATCTTTTGAGCAAACTGTGCCGACTGATGCAAGTAGAGGAAGAACTCAGAAGAAAGTACCTGTTACTTGTTCGGGTGTATCGGCCCGGCAAAGGAGAATGAGCTCTAAGAGGAAGATACAGAGTAAAAACTGTTTTATGGTTCAACCAAAAATCAGTAAGCAGCTAAGATCTACCATGAGTCCTGAGCTGCTCCAAACCCGTTTGAAGGGCTATGTGAAAGGTTTAGTGAGTGAAGAGCTACTTTCTCATGCTGAAGTCGTACGTCTTTCGAAGAAAATTAAAGTCGGTCTCGCGTTGGAGGAGCGTAAAACCAGACTGAAGGAAAGACTAGGATGTGAGCCCTCTGAAGACCAACTGGCAATATCGCTTAAGATTTCTCGCACAGAGTTACGTTCGAGAGTAATTGAGTGTTCATTGGCGAAGGAGAAGCTAGCAATGAGCAACGTTCGTTTGGTAATGTCGATTGCACAAAGATATGACAATATGGGGGCTGAAATGGCTGACTTAGTTCAGGGTGGCTTGATTGGACTTCTCCGCGGGATCGAAAAGTTTGATTCCTCAAAGGGGTTCAGAATCTCAACCTATGTTTATTGGTGGATACGCCAGGGTGTTTCAAGAGCATTAGTTGAGAATTCAAGAACGCTGAGATTGCCAACTCATTTGCACGAGAGATTAGGATTAATTCGCAATGCCAAAGTTAGACTGCAAGAGAAGGGAATCACTCCATCACTTGATAGGATTGCAGAATCCCTCAACATGTCCAAAAAGAAAGTCAAGAATGCCACTGAGGCAATCAGCAAGGTGTTCTCTTTAGACCGTGAAGCATTCCCTTCATTGAATGGTCTTCCTGGCGAAACTCATCATAGTTACATTGCGGATAATCGCCTTGAGAACGACCCGTGGCATGGCACAGACACGTGGATTCTGAAGGCTGAGGTAAACAAACTCATCAATATGACGCTTTGCGACCGAGAAAGAGAGATCATTCGTCTTTACCACGGTCTGGATAACGAATGTCTGACATGGGAGGAAATAAGCAAACGCATAGGGTTGTCCAGAGAAAGGGTAAGACAAGTTGGACTGGTGGCATTGGAGAAACTAAAGAAGGCAGCCAAGACAAGGAAGATGGAAGCCATGCTGCTCAAACAT

Protein sequence

MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSPSFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLEKQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQLRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPSEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRLQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNRLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRERVRQVGLVALEKLKKAAKTRKMEAMLLKH
Homology
BLAST of MS009066 vs. NCBI nr
Match: XP_022148631.1 (RNA polymerase sigma factor sigA [Momordica charantia] >XP_022148632.1 RNA polymerase sigma factor sigA [Momordica charantia])

HSP 1 Score: 970.3 bits (2507), Expect = 6.4e-279
Identity = 505/508 (99.41%), Postives = 506/508 (99.61%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP
Sbjct: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60

Query: 61  SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE 120
           SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE
Sbjct: 61  SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE 120

Query: 121 KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL 180
           KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL
Sbjct: 121 KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL 180

Query: 181 RSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS 240
           RST+SPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS
Sbjct: 181 RSTISPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS 240

Query: 241 EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300
           EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI
Sbjct: 241 EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300

Query: 301 GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL 360
           GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL
Sbjct: 301 GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL 360

Query: 361 QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420
           QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 
Sbjct: 361 QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNX 420

Query: 421 LENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480
           LENDPWHGTDTWILKAEVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRER
Sbjct: 421 LENDPWHGTDTWILKAEVNKLINMTLGDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480

Query: 481 VRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           VRQVGLVALEKLKKAAKTRKMEAMLLKH
Sbjct: 481 VRQVGLVALEKLKKAAKTRKMEAMLLKH 508

BLAST of MS009066 vs. NCBI nr
Match: XP_038902049.1 (RNA polymerase sigma factor sigA [Benincasa hispida])

HSP 1 Score: 921.0 bits (2379), Expect = 4.5e-264
Identity = 480/509 (94.30%), Postives = 494/509 (97.05%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYA-NDHRLGQSQVSPTKSVVIAKSSANFS 60
           MMATAAIIGLGAAKRLLNSSSYYSDF EK+LY  NDHRLGQ+QVSPTKSVVIAKSSANFS
Sbjct: 1   MMATAAIIGLGAAKRLLNSSSYYSDFTEKILYVNNDHRLGQTQVSPTKSVVIAKSSANFS 60

Query: 61  PSFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSML 120
           PS+PSSNRH+QCIKAVKEHVDTPSSPT EPWL N+T WEDEEIDLKCTVEALLLLQKSML
Sbjct: 61  PSYPSSNRHSQCIKAVKEHVDTPSSPTAEPWLHNTTIWEDEEIDLKCTVEALLLLQKSML 120

Query: 121 EKQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQ 180
           EKQWNLSFEQTV TDA RG+T+KKVPVTCSGVSARQRRMSSKRKIQSK+ FM QPKISKQ
Sbjct: 121 EKQWNLSFEQTVSTDAPRGKTEKKVPVTCSGVSARQRRMSSKRKIQSKHVFMAQPKISKQ 180

Query: 181 LRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEP 240
           LR T+SPELLQ RLKGYVKGL+SEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEP
Sbjct: 181 LRPTISPELLQNRLKGYVKGLLSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEP 240

Query: 241 SEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL 300
           SEDQLAISLKISR ELRSRV+ECSLA+EKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL
Sbjct: 241 SEDQLAISLKISRAELRSRVMECSLAREKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL 300

Query: 301 IGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVR 360
           IGLLRGIEKFDSSKGF+ISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVR
Sbjct: 301 IGLLRGIEKFDSSKGFKISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVR 360

Query: 361 LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 420
           LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN
Sbjct: 361 LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 420

Query: 421 RLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRE 480
            LEN+PWHGTDTWILKAEVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRE
Sbjct: 421 CLENNPWHGTDTWILKAEVNKLINMTLGDREREIIRLYHGLDNECLTWEEISKRIGLSRE 480

Query: 481 RVRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           RVRQVGLVALEKLKKAAKTRKMEAMLLKH
Sbjct: 481 RVRQVGLVALEKLKKAAKTRKMEAMLLKH 509

BLAST of MS009066 vs. NCBI nr
Match: XP_016902935.1 (PREDICTED: RNA polymerase sigma factor sigA [Cucumis melo] >KAA0066336.1 RNA polymerase sigma factor sigA [Cucumis melo var. makuwa] >TYK00943.1 RNA polymerase sigma factor sigA [Cucumis melo var. makuwa])

HSP 1 Score: 901.0 bits (2327), Expect = 4.8e-258
Identity = 470/509 (92.34%), Postives = 488/509 (95.87%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMAT AIIGLGAAKRLLNSSSYYSDF EK+LYAND+RLGQ+QVSPTKSVVIAKSSANFSP
Sbjct: 1   MMATTAIIGLGAAKRLLNSSSYYSDFTEKILYANDYRLGQTQVSPTKSVVIAKSSANFSP 60

Query: 61  SFPSSNRHT-QCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSML 120
           S+P SNRH+ QCIKAVKEHV+TPSSPT EPWL N+T WEDEE DLKCTVEALLLLQKSML
Sbjct: 61  SYPLSNRHSQQCIKAVKEHVETPSSPTAEPWLPNTTIWEDEETDLKCTVEALLLLQKSML 120

Query: 121 EKQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQ 180
           EKQWNLSFEQTV TD  R +T KKVPVTCSGVSARQRRMSSKRKIQSK+ FM QPKISKQ
Sbjct: 121 EKQWNLSFEQTVSTDMPREKTLKKVPVTCSGVSARQRRMSSKRKIQSKHVFMAQPKISKQ 180

Query: 181 LRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEP 240
           LR T+SPELLQ RLKGYVKGL+SEELLSHAEVVRLSKKIKVGLALEE KTRLKERLGCEP
Sbjct: 181 LRPTISPELLQNRLKGYVKGLLSEELLSHAEVVRLSKKIKVGLALEEHKTRLKERLGCEP 240

Query: 241 SEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL 300
           SEDQLAISLKISR ELRSR++ECSLA+EKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL
Sbjct: 241 SEDQLAISLKISRAELRSRMMECSLAREKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL 300

Query: 301 IGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVR 360
           IGLLRGIEKFDSSKGF+ISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKV+
Sbjct: 301 IGLLRGIEKFDSSKGFKISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVK 360

Query: 361 LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 420
           LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN
Sbjct: 361 LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 420

Query: 421 RLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRE 480
            LEN+PWHGTDTWILKAEVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRE
Sbjct: 421 CLENNPWHGTDTWILKAEVNKLINMTLGDREREIIRLYHGLDNECLTWEEISKRIGLSRE 480

Query: 481 RVRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           RVRQVGLVALEKLKKAAKTRKMEAML+KH
Sbjct: 481 RVRQVGLVALEKLKKAAKTRKMEAMLIKH 509

BLAST of MS009066 vs. NCBI nr
Match: KAG6605170.1 (RNA polymerase sigma factor sigA, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035161.1 RNA polymerase sigma factor sigA [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 893.3 bits (2307), Expect = 1.0e-255
Identity = 462/508 (90.94%), Postives = 484/508 (95.28%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMATAAIIGLGAAKRLLNSSSYYSDF EK+LY NDHRLGQ+QVS TKSVVIAK SA+FSP
Sbjct: 1   MMATAAIIGLGAAKRLLNSSSYYSDFTEKVLYLNDHRLGQNQVSTTKSVVIAKHSADFSP 60

Query: 61  SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE 120
           S+P SNRH QCIKAVKEHVDTPSSPT EPWL N+T WEDEE DLKCTVEALLLLQKSMLE
Sbjct: 61  SYPPSNRHVQCIKAVKEHVDTPSSPTAEPWLHNTTIWEDEETDLKCTVEALLLLQKSMLE 120

Query: 121 KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL 180
           KQWNLSFEQTV TDA RG+T+KKVPVTCSGVSARQRRMSSKRKIQSK+ FM QPKISKQL
Sbjct: 121 KQWNLSFEQTVSTDAPRGKTEKKVPVTCSGVSARQRRMSSKRKIQSKHVFMAQPKISKQL 180

Query: 181 RSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS 240
           + ++SPELLQ RL+GYVKGLVSEE+L H+EVVRLSKKIKVGLALEE KTRLKERL CEPS
Sbjct: 181 KPSISPELLQNRLRGYVKGLVSEEILPHSEVVRLSKKIKVGLALEEHKTRLKERLRCEPS 240

Query: 241 EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300
           E+QLAISL+ISR ELRSRV+ECSLA+EKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI
Sbjct: 241 EEQLAISLEISRAELRSRVMECSLAREKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300

Query: 301 GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL 360
           GLLRGIEKFDSSKGF+ISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKV+L
Sbjct: 301 GLLRGIEKFDSSKGFKISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVKL 360

Query: 361 QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420
           QEKGITPS+DRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR
Sbjct: 361 QEKGITPSIDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420

Query: 421 LENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480
           LEN+PWHGTDTW LK EVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRER
Sbjct: 421 LENNPWHGTDTWSLKIEVNKLINMTLEDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480

Query: 481 VRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           VRQVGLVALEKLKKAAK RKMEAMLLKH
Sbjct: 481 VRQVGLVALEKLKKAAKMRKMEAMLLKH 508

BLAST of MS009066 vs. NCBI nr
Match: XP_023533104.1 (RNA polymerase sigma factor sigA-like [Cucurbita pepo subsp. pepo] >XP_023533105.1 RNA polymerase sigma factor sigA-like [Cucurbita pepo subsp. pepo] >XP_023533106.1 RNA polymerase sigma factor sigA-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 892.5 bits (2305), Expect = 1.7e-255
Identity = 461/508 (90.75%), Postives = 484/508 (95.28%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMATAAIIGLGAAKRLLNSSSYYSDF EK+LY NDHRLGQ+QVS TKSVVIAK SA+FSP
Sbjct: 1   MMATAAIIGLGAAKRLLNSSSYYSDFTEKVLYLNDHRLGQNQVSTTKSVVIAKHSADFSP 60

Query: 61  SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE 120
           S+P SNRH QCIKAVKEHVDTPSSPT EPWL N+T WEDEE DLKCTVEALLLLQKSMLE
Sbjct: 61  SYPPSNRHVQCIKAVKEHVDTPSSPTAEPWLHNTTIWEDEETDLKCTVEALLLLQKSMLE 120

Query: 121 KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL 180
           KQWNLSFEQTV TD  RG+T+KKVPVTCSGVSARQRRMSSKRKIQSK+ F+ QPKISKQL
Sbjct: 121 KQWNLSFEQTVSTDVPRGKTEKKVPVTCSGVSARQRRMSSKRKIQSKHVFIAQPKISKQL 180

Query: 181 RSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS 240
           + ++SPELLQ RL+GYVKGLVSEE+L H+EVVRLSKKIKVGLALEE KTRLKERL CEPS
Sbjct: 181 KPSISPELLQNRLRGYVKGLVSEEILPHSEVVRLSKKIKVGLALEEHKTRLKERLRCEPS 240

Query: 241 EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300
           E+QLAISL+ISR ELRSRV+ECSLA+EKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI
Sbjct: 241 EEQLAISLEISRAELRSRVMECSLAREKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300

Query: 301 GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL 360
           GLLRGIEKFDSSKGF+ISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKV+L
Sbjct: 301 GLLRGIEKFDSSKGFKISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVKL 360

Query: 361 QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420
           QEKGITPS+DRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR
Sbjct: 361 QEKGITPSIDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420

Query: 421 LENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480
           LEN+PWHGTDTWILK EVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRER
Sbjct: 421 LENNPWHGTDTWILKIEVNKLINMTLEDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480

Query: 481 VRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           VRQVGLVALEKLKKAAK RKMEAMLLKH
Sbjct: 481 VRQVGLVALEKLKKAAKMRKMEAMLLKH 508

BLAST of MS009066 vs. ExPASy Swiss-Prot
Match: O24629 (RNA polymerase sigma factor sigA OS=Arabidopsis thaliana OX=3702 GN=SIGA PE=1 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.3e-189
Identity = 358/519 (68.98%), Postives = 424/519 (81.70%), Query Frame = 0

Query: 2   MATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSPS 61
           MATAA+IGL   KRLL+SS Y+SD  EK L  NDH   Q  ++ TKS + AK ++N+SPS
Sbjct: 1   MATAAVIGLNTGKRLLSSSFYHSDVTEKFLSVNDHCSSQYHIASTKSGITAKKASNYSPS 60

Query: 62  FPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKC---------TVEALL 121
           FPSSNRHTQ  KA+KE VD  S  T +PWL N T   D+E++ +C         +VEA+L
Sbjct: 61  FPSSNRHTQSAKALKESVDVAS--TEKPWLPNGT---DKELEEECYDDDDLISHSVEAIL 120

Query: 122 LLQKSMLEKQWNLSFEQTVPTDASRGRT--QKKVPV-TCSGVSARQRRMSSKRKIQSKNC 181
           LLQKSMLEK WNLSFE+ V ++     T  +KK+PV TCSG+SARQRR+ +K+K    + 
Sbjct: 121 LLQKSMLEKSWNLSFEKAVSSEYPGKGTIRKKKIPVITCSGISARQRRIGAKKKTNMTHV 180

Query: 182 FMVQPKISKQLRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKT 241
             V    S +            +++GYVKG++SE++LSH EVVRLSKKIK GL L++ K+
Sbjct: 181 KAVSDVSSGK------------QVRGYVKGVISEDVLSHVEVVRLSKKIKSGLRLDDHKS 240

Query: 242 RLKERLGCEPSEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGA 301
           RLK+RLGCEPS++QLA+SLKISR EL++ ++EC LA+EKLAMSNVRLVMSIAQRYDN+GA
Sbjct: 241 RLKDRLGCEPSDEQLAVSLKISRAELQAWLMECHLAREKLAMSNVRLVMSIAQRYDNLGA 300

Query: 302 EMADLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHER 361
           EM+DLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALV+NSRTLRLPTHLHER
Sbjct: 301 EMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVDNSRTLRLPTHLHER 360

Query: 362 LGLIRNAKVRLQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPG 421
           LGLIRNAK+RLQEKGITPS+DRIAESLNMS+KKV+NATEA+SKVFSLDR+AFPSLNGLPG
Sbjct: 361 LGLIRNAKLRLQEKGITPSIDRIAESLNMSQKKVRNATEAVSKVFSLDRDAFPSLNGLPG 420

Query: 422 ETHHSYIADNRLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEE 481
           ETHHSYIAD RLEN+PWHG D   LK EV+KLI+ TL +RE+EIIRLY+GLD ECLTWE+
Sbjct: 421 ETHHSYIADTRLENNPWHGYDDLALKEEVSKLISATLGEREKEIIRLYYGLDKECLTWED 480

Query: 482 ISKRIGLSRERVRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           ISKRIGLSRERVRQVGLVALEKLK AA+ RKMEAM+LK+
Sbjct: 481 ISKRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN 502

BLAST of MS009066 vs. ExPASy Swiss-Prot
Match: Q0J7T6 (RNA polymerase sigma factor sigA OS=Oryza sativa subsp. japonica OX=39947 GN=SIGA PE=2 SV=2)

HSP 1 Score: 471.9 bits (1213), Expect = 9.4e-132
Identity = 274/532 (51.50%), Postives = 361/532 (67.86%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSD-FPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFS 60
           M AT A+IGL A  RLL++S   +D  P+K+       LG               + +F+
Sbjct: 1   MTATPAVIGLSAGNRLLSASFGPTDLMPDKV------SLGVGGGGGGGGGGGGGDAMSFA 60

Query: 61  PSFPSSNRHT---------------QCIKAVKEHVD-----TPSSPTVEPWLQNSTQWED 120
           P  P++ + T               Q ++A++ H        P  P   P   +      
Sbjct: 61  PPAPATPKLTAVAAHRLKLSPHGRAQVMRALRHHSSAAAALAPPPPPPPPPTPSPASRAA 120

Query: 121 EEIDLKCTVEALLLLQKSMLEKQWNLSFEQTVPTDA------SRGRTQKKVPVTCSGVSA 180
              DL+ ++EA++LLQ+SMLEKQW L F+      A          ++  V V  S VSA
Sbjct: 121 HAHDLESSLEAIVLLQRSMLEKQWELPFDDDNHAMAIGLAEDDDDTSKATVVVARSSVSA 180

Query: 181 RQRRMSSKRKIQSKNCFMVQPKISKQLRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVR 240
           RQRRMS +R+ ++KN               +SPEL+Q+R + Y++G VS+ELL+H +VV 
Sbjct: 181 RQRRMSGRRRGRTKN---------GAAHFAVSPELIQSRNRIYLRGTVSKELLTHKQVVH 240

Query: 241 LSKKIKVGLALEERKTRLKERLGCEPSEDQLAISLKISRTELRSRVIECSLAKEKLAMSN 300
           LS KIK G+ L++++++LKE+LG EPS  QLA SLKIS  ELRSR+ E  LA+E L MSN
Sbjct: 241 LSHKIKDGIWLQQQRSKLKEKLGNEPSYKQLAHSLKISPPELRSRMHESFLAREMLTMSN 300

Query: 301 VRLVMSIAQRYDNMGAEMADLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRAL 360
           +RLV+SIAQ+YDN+G E+ADL+QGGLIGLLRGIEKFD+S+GFRISTYVYWWIRQGVSRAL
Sbjct: 301 LRLVISIAQKYDNLGVELADLIQGGLIGLLRGIEKFDASRGFRISTYVYWWIRQGVSRAL 360

Query: 361 VENSRTLRLPTHLHERLGLIRNAKVRLQEKGITPSLDRIAESLNMSKKKVKNATEAISKV 420
            ENS+T RLPT+LHERL  IR AK  L+++GI P+++ IA SLN+S+KKV NATEA++KV
Sbjct: 361 AENSKTFRLPTYLHERLIAIRGAKYELEDQGIAPTIENIAGSLNISEKKVLNATEAVNKV 420

Query: 421 FSLDREAFPSLNGLPGETHHSYIADNRLENDPWHGTDTWILKAEVNKLINMTLCDREREI 480
            SLD++AFPSLNGLPGET HSYI D  + NDPWHG + W LK EVNKL+N TL +RER+I
Sbjct: 421 LSLDQQAFPSLNGLPGETLHSYIEDQNVANDPWHGFEEWYLKEEVNKLLNSTLNERERDI 480

Query: 481 IRLYHGLDNECLTWEEISKRIGLSRERVRQVGLVALEKLKKAAKTRKMEAML 506
           IRLYHG+  +C TWE+IS++ GLSRERVRQVGL+A+EKLK AA+ + +EA+L
Sbjct: 481 IRLYHGIGKQCHTWEDISRQFGLSRERVRQVGLIAMEKLKHAARRKNLEALL 517

BLAST of MS009066 vs. ExPASy Swiss-Prot
Match: P26683 (RNA polymerase sigma factor SigA OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=sigA PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.9e-40
Identity = 112/326 (34.36%), Postives = 185/326 (56.75%), Query Frame = 0

Query: 175 KISKQLRSTMSPELLQTR--LKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLK 234
           K +K  R T S +   T   ++ Y++ +    LL   E + L++KI   L LE  + RL 
Sbjct: 62  KAAKSRRRTQSKKKHYTEDSIRLYLQEIGRIRLLRADEEIELARKIADLLELERVRERLS 121

Query: 235 ERLGCEPSEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMA 294
           E+L  +P + + A ++++     R R+     AK+K+  SN+RLV+SIA++Y N G    
Sbjct: 122 EKLERDPRDSEWAEAVQLPLPAFRYRLHIGRRAKDKMVQSNLRLVVSIAKKYMNRGLSFQ 181

Query: 295 DLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGL 354
           DL+Q G +GL+R  EKFD  KG++ STY  WWIRQ ++RA+ + SRT+RLP HL+E +  
Sbjct: 182 DLIQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISR 241

Query: 355 I-RNAKVRLQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGET 414
           I +  K+  QE G  P+ + IA  + M+ +K++   ++     SL+           G+ 
Sbjct: 242 IKKTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPI--------GKE 301

Query: 415 HHSYIADNRLEND---PWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDN-ECLTW 474
             S + D  +E+D   P       +L+ ++ K+++ +L  RER+++RL +GLD+    T 
Sbjct: 302 EDSRLGD-FIESDGETPEDQVSKNLLREDLEKVLD-SLSPRERDVLRLRYGLDDGRMKTL 361

Query: 475 EEISKRIGLSRERVRQVGLVALEKLK 494
           EEI +   ++RER+RQ+   AL KL+
Sbjct: 362 EEIGQIFNVTRERIRQIEAKALRKLR 377

BLAST of MS009066 vs. ExPASy Swiss-Prot
Match: P38023 (RNA polymerase sigma factor SigA1 OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=sigA1 PE=3 SV=2)

HSP 1 Score: 166.0 bits (419), Expect = 1.1e-39
Identity = 115/328 (35.06%), Postives = 179/328 (54.57%), Query Frame = 0

Query: 175 KISKQLRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKER 234
           K   ++R T + + ++     Y++ +    LL   E + L+++I   LALE  +  L E+
Sbjct: 77  KAKAKVRKTYTEDSIRL----YLQEIGRIRLLRADEEIELARQIADLLALERIRDELLEQ 136

Query: 235 LGCEPSEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADL 294
           L   PS+ + A ++     E R R+     AK+K+  SN+RLV+SIA++Y N G    DL
Sbjct: 137 LDRLPSDAEWAAAVDSPLDEFRRRLFRGRRAKDKMVQSNLRLVVSIAKKYMNRGLSFQDL 196

Query: 295 VQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLI- 354
           +Q G +GL+R  EKFD  KG++ STY  WWIRQ ++RA+ + SRT+RLP HL+E +  I 
Sbjct: 197 IQEGSLGLIRAAEKFDHEKGYKFSTYATWWIRQAITRAIADQSRTIRLPVHLYETISRIK 256

Query: 355 RNAKVRLQEKGITPSLDRIAESLNMSKKKV----KNATEAISKVFSLDREAFPSLNGL-- 414
           +  K+  QE G  P+ + IA  + M+ +K+    K+A   IS    + +E    L     
Sbjct: 257 KTTKLLSQEMGRKPTEEEIATRMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIE 316

Query: 415 -PGETHHSYIADNRLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDN-ECL 474
             GET    +A N L  D         L+  ++     TL  RER+++RL +GLD+    
Sbjct: 317 ADGETPEDEVAKNLLRED---------LEGVLS-----TLSPRERDVLRLRYGLDDGRMK 376

Query: 475 TWEEISKRIGLSRERVRQVGLVALEKLK 494
           T EEI +   ++RER+RQ+   AL KL+
Sbjct: 377 TLEEIGQLFNVTRERIRQIEAKALRKLR 386

BLAST of MS009066 vs. ExPASy Swiss-Prot
Match: Q31QG5 (RNA polymerase sigma factor SigA3 OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=sigA3 PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.1e-39
Identity = 107/309 (34.63%), Postives = 183/309 (59.22%), Query Frame = 0

Query: 193 LKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEE-RKTRLKERLGCEPSEDQLAISLKIS 252
           ++ Y++ +    LL+H E V L K+++  + L+E R+T   E    +PS+ + A +  +S
Sbjct: 12  VRTYLREIGRVPLLTHEEEVVLGKRVQQWMKLQELRQTLQSEEGDRDPSDLEWAAAAGLS 71

Query: 253 RTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLIGLLRGIEKFDS 312
             ELR +      AK K+  +N+RLV+S+A++Y     ++ DL+Q G IG+ RG+EKFD 
Sbjct: 72  IEELRQQQHLGEQAKRKMIEANLRLVVSVAKKYLKRNMDLLDLIQEGTIGMQRGVEKFDP 131

Query: 313 SKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL-QEKGITPSLD 372
           +KG+R STY YWWIRQ ++RA+ E +RT+RLP H+ E+L  I+  + +L Q+ G T +L 
Sbjct: 132 TKGYRFSTYAYWWIRQAITRAIAEKARTIRLPIHITEKLNKIKKMQRQLSQQLGRTATLP 191

Query: 373 RIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNRLEND---PWH 432
            +A +L+++  +V++  E      SLD           G+   + + D  LE D   P  
Sbjct: 192 ELAAALDLTPAQVRDYLEKARHPLSLDLRV--------GDNQDTELGD-LLETDCTTPEE 251

Query: 433 GTDTWILKAEVNKLINMTLCDREREIIRLYHGL-DNECLTWEEISKRIGLSRERVRQVGL 492
            T    ++ ++++L++  L +++R++I L +GL D + +T   I  R+ +SRERVRQ+  
Sbjct: 252 FTLQASMRRDIDRLLDQ-LTEQQRQVIALRYGLEDGQVMTLASIGDRLQISRERVRQLER 310

Query: 493 VALEKLKKA 496
            AL  L++A
Sbjct: 312 EALHTLRQA 310

BLAST of MS009066 vs. ExPASy TrEMBL
Match: A0A6J1D604 (RNA polymerase sigma factor sigA OS=Momordica charantia OX=3673 GN=LOC111017241 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.1e-279
Identity = 505/508 (99.41%), Postives = 506/508 (99.61%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP
Sbjct: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60

Query: 61  SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE 120
           SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE
Sbjct: 61  SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE 120

Query: 121 KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL 180
           KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL
Sbjct: 121 KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL 180

Query: 181 RSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS 240
           RST+SPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS
Sbjct: 181 RSTISPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS 240

Query: 241 EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300
           EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI
Sbjct: 241 EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300

Query: 301 GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL 360
           GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL
Sbjct: 301 GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL 360

Query: 361 QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420
           QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 
Sbjct: 361 QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNX 420

Query: 421 LENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480
           LENDPWHGTDTWILKAEVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRER
Sbjct: 421 LENDPWHGTDTWILKAEVNKLINMTLGDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480

Query: 481 VRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           VRQVGLVALEKLKKAAKTRKMEAMLLKH
Sbjct: 481 VRQVGLVALEKLKKAAKTRKMEAMLLKH 508

BLAST of MS009066 vs. ExPASy TrEMBL
Match: A0A5D3BME8 (RNA polymerase sigma factor sigA OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001180 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.3e-258
Identity = 470/509 (92.34%), Postives = 488/509 (95.87%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMAT AIIGLGAAKRLLNSSSYYSDF EK+LYAND+RLGQ+QVSPTKSVVIAKSSANFSP
Sbjct: 1   MMATTAIIGLGAAKRLLNSSSYYSDFTEKILYANDYRLGQTQVSPTKSVVIAKSSANFSP 60

Query: 61  SFPSSNRHT-QCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSML 120
           S+P SNRH+ QCIKAVKEHV+TPSSPT EPWL N+T WEDEE DLKCTVEALLLLQKSML
Sbjct: 61  SYPLSNRHSQQCIKAVKEHVETPSSPTAEPWLPNTTIWEDEETDLKCTVEALLLLQKSML 120

Query: 121 EKQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQ 180
           EKQWNLSFEQTV TD  R +T KKVPVTCSGVSARQRRMSSKRKIQSK+ FM QPKISKQ
Sbjct: 121 EKQWNLSFEQTVSTDMPREKTLKKVPVTCSGVSARQRRMSSKRKIQSKHVFMAQPKISKQ 180

Query: 181 LRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEP 240
           LR T+SPELLQ RLKGYVKGL+SEELLSHAEVVRLSKKIKVGLALEE KTRLKERLGCEP
Sbjct: 181 LRPTISPELLQNRLKGYVKGLLSEELLSHAEVVRLSKKIKVGLALEEHKTRLKERLGCEP 240

Query: 241 SEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL 300
           SEDQLAISLKISR ELRSR++ECSLA+EKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL
Sbjct: 241 SEDQLAISLKISRAELRSRMMECSLAREKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL 300

Query: 301 IGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVR 360
           IGLLRGIEKFDSSKGF+ISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKV+
Sbjct: 301 IGLLRGIEKFDSSKGFKISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVK 360

Query: 361 LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 420
           LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN
Sbjct: 361 LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 420

Query: 421 RLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRE 480
            LEN+PWHGTDTWILKAEVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRE
Sbjct: 421 CLENNPWHGTDTWILKAEVNKLINMTLGDREREIIRLYHGLDNECLTWEEISKRIGLSRE 480

Query: 481 RVRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           RVRQVGLVALEKLKKAAKTRKMEAML+KH
Sbjct: 481 RVRQVGLVALEKLKKAAKTRKMEAMLIKH 509

BLAST of MS009066 vs. ExPASy TrEMBL
Match: A0A1S4E4N5 (RNA polymerase sigma factor sigA OS=Cucumis melo OX=3656 GN=LOC103501092 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.3e-258
Identity = 470/509 (92.34%), Postives = 488/509 (95.87%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMAT AIIGLGAAKRLLNSSSYYSDF EK+LYAND+RLGQ+QVSPTKSVVIAKSSANFSP
Sbjct: 1   MMATTAIIGLGAAKRLLNSSSYYSDFTEKILYANDYRLGQTQVSPTKSVVIAKSSANFSP 60

Query: 61  SFPSSNRHT-QCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSML 120
           S+P SNRH+ QCIKAVKEHV+TPSSPT EPWL N+T WEDEE DLKCTVEALLLLQKSML
Sbjct: 61  SYPLSNRHSQQCIKAVKEHVETPSSPTAEPWLPNTTIWEDEETDLKCTVEALLLLQKSML 120

Query: 121 EKQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQ 180
           EKQWNLSFEQTV TD  R +T KKVPVTCSGVSARQRRMSSKRKIQSK+ FM QPKISKQ
Sbjct: 121 EKQWNLSFEQTVSTDMPREKTLKKVPVTCSGVSARQRRMSSKRKIQSKHVFMAQPKISKQ 180

Query: 181 LRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEP 240
           LR T+SPELLQ RLKGYVKGL+SEELLSHAEVVRLSKKIKVGLALEE KTRLKERLGCEP
Sbjct: 181 LRPTISPELLQNRLKGYVKGLLSEELLSHAEVVRLSKKIKVGLALEEHKTRLKERLGCEP 240

Query: 241 SEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL 300
           SEDQLAISLKISR ELRSR++ECSLA+EKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL
Sbjct: 241 SEDQLAISLKISRAELRSRMMECSLAREKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGL 300

Query: 301 IGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVR 360
           IGLLRGIEKFDSSKGF+ISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKV+
Sbjct: 301 IGLLRGIEKFDSSKGFKISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVK 360

Query: 361 LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 420
           LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN
Sbjct: 361 LQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADN 420

Query: 421 RLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRE 480
            LEN+PWHGTDTWILKAEVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRE
Sbjct: 421 CLENNPWHGTDTWILKAEVNKLINMTLGDREREIIRLYHGLDNECLTWEEISKRIGLSRE 480

Query: 481 RVRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           RVRQVGLVALEKLKKAAKTRKMEAML+KH
Sbjct: 481 RVRQVGLVALEKLKKAAKTRKMEAMLIKH 509

BLAST of MS009066 vs. ExPASy TrEMBL
Match: A0A6J1KWY7 (RNA polymerase sigma factor sigA-like OS=Cucurbita maxima OX=3661 GN=LOC111499522 PE=3 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 7.0e-255
Identity = 460/508 (90.55%), Postives = 482/508 (94.88%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMATAAIIGLGAAKRLLNSSSYYSDF EK+LY NDHRLGQ+QVS TK VVIAK SA+FSP
Sbjct: 1   MMATAAIIGLGAAKRLLNSSSYYSDFTEKVLYLNDHRLGQNQVSTTKGVVIAKHSADFSP 60

Query: 61  SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE 120
           S+  SNRH QCIKAVKEHVDTPSSPT EPWL N+T WEDEE DLKCTVEALLLLQKSMLE
Sbjct: 61  SYSPSNRHVQCIKAVKEHVDTPSSPTAEPWLHNTTIWEDEETDLKCTVEALLLLQKSMLE 120

Query: 121 KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL 180
           KQWNLSFEQTV TDA RG+T+KKVPVTCSGVSARQRRMSSKRKIQSK+ FM QPKISKQL
Sbjct: 121 KQWNLSFEQTVSTDAPRGKTEKKVPVTCSGVSARQRRMSSKRKIQSKHVFMAQPKISKQL 180

Query: 181 RSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS 240
           +  +SPELLQ RL+GYVKGLVSEE+L H+EVVRLSKKIKVGLALEE KTRLKERL C+PS
Sbjct: 181 KPNISPELLQNRLRGYVKGLVSEEILPHSEVVRLSKKIKVGLALEEHKTRLKERLRCDPS 240

Query: 241 EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300
           E+QLAISL+ISR ELRSRV+ECSLA+EKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI
Sbjct: 241 EEQLAISLEISRAELRSRVMECSLAREKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300

Query: 301 GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL 360
           GLLRGIEKFDSSKGF+ISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKV+L
Sbjct: 301 GLLRGIEKFDSSKGFKISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVKL 360

Query: 361 QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420
           QEKGITPS+DRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR
Sbjct: 361 QEKGITPSIDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420

Query: 421 LENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480
           LEN+PWHGTDTWILK EVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRER
Sbjct: 421 LENNPWHGTDTWILKIEVNKLINMTLEDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480

Query: 481 VRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           VRQVGLVALEKLKKAAK RKMEAMLLKH
Sbjct: 481 VRQVGLVALEKLKKAAKMRKMEAMLLKH 508

BLAST of MS009066 vs. ExPASy TrEMBL
Match: A0A6J1G739 (RNA polymerase sigma factor sigA-like OS=Cucurbita moschata OX=3662 GN=LOC111451427 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.3e-253
Identity = 458/508 (90.16%), Postives = 481/508 (94.69%), Query Frame = 0

Query: 1   MMATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSP 60
           MMATAAIIGLGAAKRLLNSSSYYSDF EK+LY NDHRLGQ+QVS TKSVVIAK SA+FSP
Sbjct: 1   MMATAAIIGLGAAKRLLNSSSYYSDFTEKVLYLNDHRLGQNQVSTTKSVVIAKHSADFSP 60

Query: 61  SFPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKCTVEALLLLQKSMLE 120
           S+P SNRH QCIKAVKEHVDTPSSPT EPWL N+T WEDEE DLKCTVEALLLLQKSMLE
Sbjct: 61  SYPPSNRHVQCIKAVKEHVDTPSSPTAEPWLNNTTIWEDEETDLKCTVEALLLLQKSMLE 120

Query: 121 KQWNLSFEQTVPTDASRGRTQKKVPVTCSGVSARQRRMSSKRKIQSKNCFMVQPKISKQL 180
           KQWNLSFEQTV TD  RG+T+KKVPVTCSGVSARQRRM+SKRKIQSK+ FM QPKISKQ 
Sbjct: 121 KQWNLSFEQTVSTDVPRGKTEKKVPVTCSGVSARQRRMNSKRKIQSKHVFMAQPKISKQQ 180

Query: 181 RSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPS 240
           + ++SPELLQ RL+GYVKGLVSEE+L H+EVVRLSKKIKVGLALEE KTRLKERL CEPS
Sbjct: 181 KPSISPELLQNRLRGYVKGLVSEEILPHSEVVRLSKKIKVGLALEEHKTRLKERLRCEPS 240

Query: 241 EDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300
           E+QLAISL+IS  ELRSRV+ECSLA+EKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI
Sbjct: 241 EEQLAISLEISCAELRSRVMECSLAREKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLI 300

Query: 301 GLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL 360
           GLLRGIEKFDSSKGF+ISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKV+L
Sbjct: 301 GLLRGIEKFDSSKGFKISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVKL 360

Query: 361 QEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420
           QEKGITPS+DRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR
Sbjct: 361 QEKGITPSIDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNR 420

Query: 421 LENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480
           LEN+PWHGTDTW LK EVNKLINMTL DREREIIRLYHGLDNECLTWEEISKRIGLSRER
Sbjct: 421 LENNPWHGTDTWSLKIEVNKLINMTLEDREREIIRLYHGLDNECLTWEEISKRIGLSRER 480

Query: 481 VRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           VRQVGLVALEKLKKAAK RKMEAMLLKH
Sbjct: 481 VRQVGLVALEKLKKAAKMRKMEAMLLKH 508

BLAST of MS009066 vs. TAIR 10
Match: AT1G64860.1 (sigma factor A )

HSP 1 Score: 664.1 bits (1712), Expect = 9.2e-191
Identity = 358/519 (68.98%), Postives = 424/519 (81.70%), Query Frame = 0

Query: 2   MATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSPS 61
           MATAA+IGL   KRLL+SS Y+SD  EK L  NDH   Q  ++ TKS + AK ++N+SPS
Sbjct: 1   MATAAVIGLNTGKRLLSSSFYHSDVTEKFLSVNDHCSSQYHIASTKSGITAKKASNYSPS 60

Query: 62  FPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKC---------TVEALL 121
           FPSSNRHTQ  KA+KE VD  S  T +PWL N T   D+E++ +C         +VEA+L
Sbjct: 61  FPSSNRHTQSAKALKESVDVAS--TEKPWLPNGT---DKELEEECYDDDDLISHSVEAIL 120

Query: 122 LLQKSMLEKQWNLSFEQTVPTDASRGRT--QKKVPV-TCSGVSARQRRMSSKRKIQSKNC 181
           LLQKSMLEK WNLSFE+ V ++     T  +KK+PV TCSG+SARQRR+ +K+K    + 
Sbjct: 121 LLQKSMLEKSWNLSFEKAVSSEYPGKGTIRKKKIPVITCSGISARQRRIGAKKKTNMTHV 180

Query: 182 FMVQPKISKQLRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKT 241
             V    S +            +++GYVKG++SE++LSH EVVRLSKKIK GL L++ K+
Sbjct: 181 KAVSDVSSGK------------QVRGYVKGVISEDVLSHVEVVRLSKKIKSGLRLDDHKS 240

Query: 242 RLKERLGCEPSEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGA 301
           RLK+RLGCEPS++QLA+SLKISR EL++ ++EC LA+EKLAMSNVRLVMSIAQRYDN+GA
Sbjct: 241 RLKDRLGCEPSDEQLAVSLKISRAELQAWLMECHLAREKLAMSNVRLVMSIAQRYDNLGA 300

Query: 302 EMADLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHER 361
           EM+DLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALV+NSRTLRLPTHLHER
Sbjct: 301 EMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVDNSRTLRLPTHLHER 360

Query: 362 LGLIRNAKVRLQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPG 421
           LGLIRNAK+RLQEKGITPS+DRIAESLNMS+KKV+NATEA+SKVFSLDR+AFPSLNGLPG
Sbjct: 361 LGLIRNAKLRLQEKGITPSIDRIAESLNMSQKKVRNATEAVSKVFSLDRDAFPSLNGLPG 420

Query: 422 ETHHSYIADNRLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEE 481
           ETHHSYIAD RLEN+PWHG D   LK EV+KLI+ TL +RE+EIIRLY+GLD ECLTWE+
Sbjct: 421 ETHHSYIADTRLENNPWHGYDDLALKEEVSKLISATLGEREKEIIRLYYGLDKECLTWED 480

Query: 482 ISKRIGLSRERVRQVGLVALEKLKKAAKTRKMEAMLLKH 509
           ISKRIGLSRERVRQVGLVALEKLK AA+ RKMEAM+LK+
Sbjct: 481 ISKRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN 502

BLAST of MS009066 vs. TAIR 10
Match: AT1G64860.2 (sigma factor A )

HSP 1 Score: 615.5 bits (1586), Expect = 3.7e-176
Identity = 329/485 (67.84%), Postives = 392/485 (80.82%), Query Frame = 0

Query: 2   MATAAIIGLGAAKRLLNSSSYYSDFPEKLLYANDHRLGQSQVSPTKSVVIAKSSANFSPS 61
           MATAA+IGL   KRLL+SS Y+SD  EK L  NDH   Q  ++ TKS + AK ++N+SPS
Sbjct: 1   MATAAVIGLNTGKRLLSSSFYHSDVTEKFLSVNDHCSSQYHIASTKSGITAKKASNYSPS 60

Query: 62  FPSSNRHTQCIKAVKEHVDTPSSPTVEPWLQNSTQWEDEEIDLKC---------TVEALL 121
           FPSSNRHTQ  KA+KE VD  S  T +PWL N T   D+E++ +C         +VEA+L
Sbjct: 61  FPSSNRHTQSAKALKESVDVAS--TEKPWLPNGT---DKELEEECYDDDDLISHSVEAIL 120

Query: 122 LLQKSMLEKQWNLSFEQTVPTDASRGRT--QKKVPV-TCSGVSARQRRMSSKRKIQSKNC 181
           LLQKSMLEK WNLSFE+ V ++     T  +KK+PV TCSG+SARQRR+ +K+K    + 
Sbjct: 121 LLQKSMLEKSWNLSFEKAVSSEYPGKGTIRKKKIPVITCSGISARQRRIGAKKKTNMTHV 180

Query: 182 FMVQPKISKQLRSTMSPELLQTRLKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKT 241
             V    S +            +++GYVKG++SE++LSH EVVRLSKKIK GL L++ K+
Sbjct: 181 KAVSDVSSGK------------QVRGYVKGVISEDVLSHVEVVRLSKKIKSGLRLDDHKS 240

Query: 242 RLKERLGCEPSEDQLAISLKISRTELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGA 301
           RLK+RLGCEPS++QLA+SLKISR EL++ ++EC LA+EKLAMSNVRLVMSIAQRYDN+GA
Sbjct: 241 RLKDRLGCEPSDEQLAVSLKISRAELQAWLMECHLAREKLAMSNVRLVMSIAQRYDNLGA 300

Query: 302 EMADLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHER 361
           EM+DLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALV+NSRTLRLPTHLHER
Sbjct: 301 EMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVDNSRTLRLPTHLHER 360

Query: 362 LGLIRNAKVRLQEKGITPSLDRIAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPG 421
           LGLIRNAK+RLQEKGITPS+DRIAESLNMS+KKV+NATEA+SKVFSLDR+AFPSLNGLPG
Sbjct: 361 LGLIRNAKLRLQEKGITPSIDRIAESLNMSQKKVRNATEAVSKVFSLDRDAFPSLNGLPG 420

Query: 422 ETHHSYIADNRLENDPWHGTDTWILKAEVNKLINMTLCDREREIIRLYHGLDNECLTWEE 475
           ETHHSYIAD RLEN+PWHG D   LK EV+KLI+ TL +RE+EIIRLY+GLD ECLTWE+
Sbjct: 421 ETHHSYIADTRLENNPWHGYDDLALKEEVSKLISATLGEREKEIIRLYYGLDKECLTWED 468

BLAST of MS009066 vs. TAIR 10
Match: AT1G08540.1 (RNApolymerase sigma subunit 2 )

HSP 1 Score: 161.4 bits (407), Expect = 1.9e-39
Identity = 111/310 (35.81%), Postives = 174/310 (56.13%), Query Frame = 0

Query: 202 SEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPSEDQLAISLKISRTELRSRVIE 261
           S +LL+  E   LS  I+  L LE  +T L ER G +P+  Q A +  + +  LR R+  
Sbjct: 269 SSKLLTVREEHELSAGIQDLLKLERLQTELTERSGRQPTFAQWASAAGVDQKSLRQRIHH 328

Query: 262 CSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLIGLLRGIEKFDSSKGFRISTYV 321
            +L K+K+  SN+RLV+SIA+ Y   G  + DLVQ G  GL+RG EKFD++KGF+ STY 
Sbjct: 329 GTLCKDKMIKSNIRLVISIAKNYQGAGMNLQDLVQEGCRGLVRGAEKFDATKGFKFSTYA 388

Query: 322 YWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRL-QEKGITPSLDRIAESLNMSK 381
           +WWI+Q V ++L + SR +RLP H+ E    ++ A+ +L  E G  P  + IAE+  +S 
Sbjct: 389 HWWIKQAVRKSLSDQSRMIRLPFHMVEATYRVKEARKQLYSETGKHPKNEEIAEATGLSM 448

Query: 382 KKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNRLENDPWHGTDTWI---LKAE 441
           K++     +     SLD++   + N  P E     IAD           D  I   ++ +
Sbjct: 449 KRLMAVLLSPKPPRSLDQKIGMNQNLKPSEV----IADPEAVT----SEDILIKEFMRQD 508

Query: 442 VNKLINMTLCDREREIIRLYHGL-DNECLTWEEISKRIGLSRERVRQVGLVALEKLKKAA 501
           ++K+++ +L  RE+++IR   G+ D    T +EI + +G+SRERVRQ+   A  KLK   
Sbjct: 509 LDKVLD-SLGTREKQVIRWRFGMEDGRMKTLQEIGEMMGVSRERVRQIESSAFRKLKNKK 568

Query: 502 KTRKMEAMLL 507
           +   ++  L+
Sbjct: 569 RNNHLQQYLV 569

BLAST of MS009066 vs. TAIR 10
Match: AT2G36990.1 (RNApolymerase sigma-subunit F )

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-36
Identity = 102/313 (32.59%), Postives = 173/313 (55.27%), Query Frame = 0

Query: 193 LKGYVKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPSEDQLAISLKISR 252
           L+ ++ G  +++LL+  E   L   I+  L LE+ KT+L+ + GCEP+  + A ++ IS 
Sbjct: 235 LQLFLWGPETKQLLTAKEEAELISHIQHLLKLEKVKTKLESQNGCEPTIGEWAEAMGISS 294

Query: 253 TELRSRVIECSLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLIGLLRGIEKFDSS 312
             L+S +     ++EKL  +N+RLV+ IA++Y N G    DL+Q G +GL++ +EKF   
Sbjct: 295 PVLKSDIHRGRSSREKLITANLRLVVHIAKQYQNRGLNFQDLLQEGSMGLMKSVEKFKPQ 354

Query: 313 KGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNA-KVRLQEKGITPSLDR 372
            G R +TY YWWIRQ + +++ +NSRT+RLP +++  LG +  A K  +QE    PS + 
Sbjct: 355 SGCRFATYAYWWIRQSIRKSIFQNSRTIRLPENVYMLLGKVSEARKTCVQEGNYRPSKEE 414

Query: 373 IAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNRLENDPWHGTDT 432
           +A  + +S +K+           S+ +  +   +     T      D+ +E  P      
Sbjct: 415 LAGHVGVSTEKLDKLLYNTRTPLSMQQPIWSDQD----TTFQEITPDSGIET-PTMSVGK 474

Query: 433 WILKAEVNKLINMTLCDREREIIRLYHGLD-NECLTWEEISKRIGLSRERVRQVGLVALE 492
            +++  V  L+N+ L  +ER II+L  G+D  +  +  EI +  GLS+ERVRQ+   AL 
Sbjct: 475 QLMRNHVRNLLNV-LSPKERRIIKLRFGIDGGKQRSLSEIGEIYGLSKERVRQLESRALY 534

Query: 493 KLKKAAKTRKMEA 504
           +LK+   +  + A
Sbjct: 535 RLKQNMNSHGLHA 541

BLAST of MS009066 vs. TAIR 10
Match: AT5G13730.1 (sigma factor 4 )

HSP 1 Score: 142.1 bits (357), Expect = 1.2e-33
Identity = 106/303 (34.98%), Postives = 154/303 (50.83%), Query Frame = 0

Query: 197 VKGLVSEELLSHAEVVRLSKKIKVGLALEERKTRLKERLGCEPSEDQLAISLKISRTELR 256
           V G      LS  E V+L   +K G  LE   T ++E        + +++ L   R + +
Sbjct: 114 VVGASRSGFLSRLEEVQLCLYLKEGAKLENLGTSVEE-------NEMVSVLLASGRGKKK 173

Query: 257 SRVIEC----SLAKEKLAMSNVRLVMSIAQRYDNMGAEMADLVQGGLIGLLRGIEKFDSS 316
               E       A+EK+     RLV+SIA  Y   G  + DL+Q G IGLLRG E+FD  
Sbjct: 174 RSANEILCRRKEAREKITRCYRRLVVSIATGYQGKGLNLQDLIQEGSIGLLRGAERFDPD 233

Query: 317 KGFRISTYVYWWIRQGVSRALVENSRTLRLPTHLHERLGLIRNAKVRLQEK-GITPSLDR 376
           +G+++STYVYWWI+Q + RA+   SR ++LP  + E    +  A   L  K    PS + 
Sbjct: 234 RGYKLSTYVYWWIKQAILRAIAHKSRLVKLPGSMWELTAKVAEASNVLTRKLRRQPSCEE 293

Query: 377 IAESLNMSKKKVKNATEAISKVFSLDREAFPSLNGLPGETHHSYIADNRLENDPWHGTDT 436
           IAE LN++   V+ A E      SLDR A  +     G      I     E  P      
Sbjct: 294 IAEHLNLNVSAVRLAVERSRSPVSLDRVASQN-----GRMTLQEIVRGPDETRPEEMVKR 353

Query: 437 WILKAEVNKLINMTLCDREREIIRLYHGLDNEC-LTWEEISKRIGLSRERVRQVGLVALE 494
             +K E+ +L+  +L  RE  ++ LY GL+ E  +++EEI K + LSRERVRQ+  +AL+
Sbjct: 354 EHMKHEIEQLLG-SLTARESRVLGLYFGLNGETPMSFEEIGKSLKLSRERVRQINGIALK 403

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148631.16.4e-27999.41RNA polymerase sigma factor sigA [Momordica charantia] >XP_022148632.1 RNA polym... [more]
XP_038902049.14.5e-26494.30RNA polymerase sigma factor sigA [Benincasa hispida][more]
XP_016902935.14.8e-25892.34PREDICTED: RNA polymerase sigma factor sigA [Cucumis melo] >KAA0066336.1 RNA pol... [more]
KAG6605170.11.0e-25590.94RNA polymerase sigma factor sigA, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023533104.11.7e-25590.75RNA polymerase sigma factor sigA-like [Cucurbita pepo subsp. pepo] >XP_023533105... [more]
Match NameE-valueIdentityDescription
O246291.3e-18968.98RNA polymerase sigma factor sigA OS=Arabidopsis thaliana OX=3702 GN=SIGA PE=1 SV... [more]
Q0J7T69.4e-13251.50RNA polymerase sigma factor sigA OS=Oryza sativa subsp. japonica OX=39947 GN=SIG... [more]
P266834.9e-4034.36RNA polymerase sigma factor SigA OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UT... [more]
P380231.1e-3935.06RNA polymerase sigma factor SigA1 OS=Synechococcus elongatus (strain PCC 7942 / ... [more]
Q31QG51.1e-3934.63RNA polymerase sigma factor SigA3 OS=Synechococcus elongatus (strain PCC 7942 / ... [more]
Match NameE-valueIdentityDescription
A0A6J1D6043.1e-27999.41RNA polymerase sigma factor sigA OS=Momordica charantia OX=3673 GN=LOC111017241 ... [more]
A0A5D3BME82.3e-25892.34RNA polymerase sigma factor sigA OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S4E4N52.3e-25892.34RNA polymerase sigma factor sigA OS=Cucumis melo OX=3656 GN=LOC103501092 PE=3 SV... [more]
A0A6J1KWY77.0e-25590.55RNA polymerase sigma factor sigA-like OS=Cucurbita maxima OX=3661 GN=LOC11149952... [more]
A0A6J1G7391.3e-25390.16RNA polymerase sigma factor sigA-like OS=Cucurbita moschata OX=3662 GN=LOC111451... [more]
Match NameE-valueIdentityDescription
AT1G64860.19.2e-19168.98sigma factor A [more]
AT1G64860.23.7e-17667.84sigma factor A [more]
AT1G08540.11.9e-3935.81RNApolymerase sigma subunit 2 [more]
AT2G36990.11.5e-3632.59RNApolymerase sigma-subunit F [more]
AT5G13730.11.2e-3334.98sigma factor 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000943RNA polymerase sigma-70PRINTSPR00046SIGMA70FCTcoord: 317..325
score: 64.52
coord: 466..481
score: 48.75
coord: 481..492
score: 46.67
coord: 293..306
score: 49.68
coord: 446..458
score: 36.82
IPR000943RNA polymerase sigma-70PROSITEPS00715SIGMA70_1coord: 293..306
IPR007627RNA polymerase sigma-70 region 2PFAMPF04542Sigma70_r2coord: 271..338
e-value: 2.2E-16
score: 59.4
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 340..390
e-value: 5.9E-7
score: 31.5
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 424..502
e-value: 4.3E-20
score: 73.4
NoneNo IPR availableGENE3D1.10.601.10RNA Polymerase Primary Sigma Factorcoord: 86..339
e-value: 2.7E-32
score: 114.7
NoneNo IPR availablePANTHERPTHR30603RNA POLYMERASE SIGMA FACTOR RPOcoord: 2..508
NoneNo IPR availablePANTHERPTHR30603:SF14RNA POLYMERASE SIGMA FACTOR SIGAcoord: 2..508
NoneNo IPR availableCDDcd06171Sigma70_r4coord: 435..493
e-value: 2.96101E-7
score: 45.173
IPR014284RNA polymerase sigma-70 like domainTIGRFAMTIGR02937TIGR02937coord: 266..495
e-value: 7.2E-25
score: 85.5
IPR007624RNA polymerase sigma-70 region 3PFAMPF04539Sigma70_r3coord: 349..419
e-value: 3.0E-11
score: 43.3
IPR007630RNA polymerase sigma-70 region 4PFAMPF04545Sigma70_r4coord: 445..494
e-value: 2.4E-10
score: 39.8
IPR013324RNA polymerase sigma factor, region 3/4-likeSUPERFAMILY88659Sigma3 and sigma4 domains of RNA polymerase sigma factorscoord: 421..498
IPR013324RNA polymerase sigma factor, region 3/4-likeSUPERFAMILY88659Sigma3 and sigma4 domains of RNA polymerase sigma factorscoord: 341..397
IPR013325RNA polymerase sigma factor, region 2SUPERFAMILY88946Sigma2 domain of RNA polymerase sigma factorscoord: 193..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009066.1MS009066.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006352 DNA-templated transcription, initiation
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity