MS009048 (gene) Bitter gourd (TR) v1

Overview
NameMS009048
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPolcalcin Jun o
Locationscaffold385: 466130 .. 466663 (+)
RNA-Seq ExpressionMS009048
SyntenyMS009048
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTTCTCACGGAAGAAGAAATCGGATGCCTCGAGCGATTCGCCGCCTGGATCTACGGCGGTTCCGGTGCTTGGATCTCGAACGCCGTCTTTCAATTGTCGAACGCAGATCGCGGAACTGGAGCAAGTATTCAAGAAATTCGATGTGAACGGCGACGGCAAGATCTCCTCCTCTGAACTCGGATCCATTATGGGGAGCCTAGGGCAGCCGGCGACGGAGGAGGAGATTCAGAACATGATCAAGGAGGTGGACGCGGACGGCGACGGATTCATTGACCTCGACGAATTCATCGAGTTGAACACCAAGGGCGTGGATTCCGAGGAGGTGCTGGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGAAACGGATCGATCACGGCGGAGGAATTGCACGAGGTGTTGAAGAGTTTGGGGGACGAATGTTCGTTGGCCGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGTGACGGTATGATTAGCTTCGATGAATTCAAAGTCATGATGATGAGCGGCTCTCGA

mRNA sequence

CTCTTCTCACGGAAGAAGAAATCGGATGCCTCGAGCGATTCGCCGCCTGGATCTACGGCGGTTCCGGTGCTTGGATCTCGAACGCCGTCTTTCAATTGTCGAACGCAGATCGCGGAACTGGAGCAAGTATTCAAGAAATTCGATGTGAACGGCGACGGCAAGATCTCCTCCTCTGAACTCGGATCCATTATGGGGAGCCTAGGGCAGCCGGCGACGGAGGAGGAGATTCAGAACATGATCAAGGAGGTGGACGCGGACGGCGACGGATTCATTGACCTCGACGAATTCATCGAGTTGAACACCAAGGGCGTGGATTCCGAGGAGGTGCTGGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGAAACGGATCGATCACGGCGGAGGAATTGCACGAGGTGTTGAAGAGTTTGGGGGACGAATGTTCGTTGGCCGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGTGACGGTATGATTAGCTTCGATGAATTCAAAGTCATGATGATGAGCGGCTCTCGA

Coding sequence (CDS)

CTCTTCTCACGGAAGAAGAAATCGGATGCCTCGAGCGATTCGCCGCCTGGATCTACGGCGGTTCCGGTGCTTGGATCTCGAACGCCGTCTTTCAATTGTCGAACGCAGATCGCGGAACTGGAGCAAGTATTCAAGAAATTCGATGTGAACGGCGACGGCAAGATCTCCTCCTCTGAACTCGGATCCATTATGGGGAGCCTAGGGCAGCCGGCGACGGAGGAGGAGATTCAGAACATGATCAAGGAGGTGGACGCGGACGGCGACGGATTCATTGACCTCGACGAATTCATCGAGTTGAACACCAAGGGCGTGGATTCCGAGGAGGTGCTGGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGAAACGGATCGATCACGGCGGAGGAATTGCACGAGGTGTTGAAGAGTTTGGGGGACGAATGTTCGTTGGCCGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGTGACGGTATGATTAGCTTCGATGAATTCAAAGTCATGATGATGAGCGGCTCTCGA

Protein sequence

LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR
Homology
BLAST of MS009048 vs. NCBI nr
Match: XP_022146331.1 (probable calcium-binding protein CML25 [Momordica charantia])

HSP 1 Score: 349.4 bits (895), Expect = 1.9e-92
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL
Sbjct: 6   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR
Sbjct: 126 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 183

BLAST of MS009048 vs. NCBI nr
Match: XP_023513887.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.5 bits (820), Expect = 9.3e-84
Identity = 161/178 (90.45%), Postives = 170/178 (95.51%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI SSEL
Sbjct: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           D+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMSGSR
Sbjct: 126 DLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGSR 183

BLAST of MS009048 vs. NCBI nr
Match: XP_022963580.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 320.5 bits (820), Expect = 9.3e-84
Identity = 161/178 (90.45%), Postives = 170/178 (95.51%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI SSEL
Sbjct: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           D+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMSGSR
Sbjct: 126 DLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGSR 183

BLAST of MS009048 vs. NCBI nr
Match: XP_022990613.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 320.5 bits (820), Expect = 9.3e-84
Identity = 161/178 (90.45%), Postives = 170/178 (95.51%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI SSEL
Sbjct: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           D+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMSGSR
Sbjct: 126 DLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGSR 183

BLAST of MS009048 vs. NCBI nr
Match: KAG6601861.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032560.1 putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 318.5 bits (815), Expect = 3.5e-83
Identity = 160/178 (89.89%), Postives = 169/178 (94.94%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI SSEL
Sbjct: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMG LGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKDAFSVY
Sbjct: 66  GSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           D+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMSGSR
Sbjct: 126 DLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGSR 183

BLAST of MS009048 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 1.5e-55
Identity = 111/182 (60.99%), Postives = 141/182 (77.47%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTP----SFNCRTQIAELEQVFKKFDVNGDGKIS 60
           +F++ + S+  S S  G      +G+ +P    + + +T+I ELE VFKKFDVNGDGKIS
Sbjct: 1   MFNKNQGSNGGSSSNVG------IGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKIS 60

Query: 61  SSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDA 120
           S ELG+IM SLG    EEE++  I E+D  GDG+I+ +EF+ELNTKG+D  +VLENLKDA
Sbjct: 61  SKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 120

Query: 121 FSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSG 179
           FSVYDIDGNGSI+AEELHEVL+SLGDECS+A+CRKMI GVD++GDG I F+EFK+MM  G
Sbjct: 121 FSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 176

BLAST of MS009048 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 4.6e-41
Identity = 93/177 (52.54%), Postives = 121/177 (68.36%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LF R+    + S SPP   A P  G  +P+    T   E+E+VF+KFD NGDG+IS SEL
Sbjct: 9   LFRRR----SGSKSPPLPQADPASGGGSPA---PTPEEEMERVFRKFDANGDGRISRSEL 68

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELN-TKGVDSEEVLENLKDAFSV 120
           G++  SLG  AT++E+  M+ E DADGDGFI LDEF  LN T   D+  V E+L+ AF V
Sbjct: 69  GALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRV 128

Query: 121 YDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSG 177
           +D DGNG+I+A EL  VL  LG++ ++  CR+MI GVD+NGDG+ISF+EFKVMM  G
Sbjct: 129 FDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGG 178

BLAST of MS009048 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-35
Identity = 72/149 (48.32%), Postives = 109/149 (73.15%), Query Frame = 0

Query: 32  NCRTQIAELEQVFKKFDVNGDGKISSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFI 91
           +C   + ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M+K+ D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 92  DLDEFIELNTKGV----DSEEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLA 151
           DLDEF+ L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG++CS+ 
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 152 DCRKMITGVDRNGDGMISFDEFKVMMMSG 177
           DC+KMI+ VD +GDG ++FDEFK MM +G
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMSNG 158

BLAST of MS009048 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.2e-35
Identity = 72/151 (47.68%), Postives = 107/151 (70.86%), Query Frame = 0

Query: 26  SRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSELGSIMGSLGQPATEEEIQNMIKEVDA 85
           S+  S NC   + ++++VF++FD N DGKIS  EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 86  DGDGFIDLDEFIEL--NTKGVDSEEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDE 145
           DG+GFIDLDEF+ L   +    +   + +LK+AF +YD+D NG I+A ELH V+K+LG++
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 146 CSLADCRKMITGVDRNGDGMISFDEFKVMMM 175
           CS+ DC++MI  VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

BLAST of MS009048 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 1.9e-34
Identity = 72/139 (51.80%), Postives = 101/139 (72.66%), Query Frame = 0

Query: 37  IAELEQVFKKFDVNGDGKISSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEF 96
           + ELE+VFKKFD NGDGKIS SEL  I+ SLG    E E++ M++E DADGDG++ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 97  IELNTKGVDSEEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGV 156
           ++LN KG      +++LK+AF V+D D NGSI+A EL   L+S+G+ C++ + + +I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 157 DRNGDGMISFDEFKVMMMS 176
           D+NGDG+IS +EF+ MM S
Sbjct: 144 DKNGDGLISVEEFQTMMTS 158

BLAST of MS009048 vs. ExPASy TrEMBL
Match: A0A6J1CZ20 (probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC111015568 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.1e-93
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL
Sbjct: 6   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR
Sbjct: 126 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 183

BLAST of MS009048 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.5e-84
Identity = 161/178 (90.45%), Postives = 170/178 (95.51%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI SSEL
Sbjct: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           D+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMSGSR
Sbjct: 126 DLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGSR 183

BLAST of MS009048 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.5e-84
Identity = 161/178 (90.45%), Postives = 170/178 (95.51%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKSD+SSDSP GST VP+LGSRTPSFN R QIAELEQVFKKFDVNGDGKI SSEL
Sbjct: 6   LFSRKKKSDSSSDSPLGSTTVPILGSRTPSFNSRAQIAELEQVFKKFDVNGDGKICSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEE+QNMIKEVDADGDG+IDL EFIELNTKGVDS+EVL+NLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKNLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           D+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK MMMSGSR
Sbjct: 126 DLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAMMMSGSR 183

BLAST of MS009048 vs. ExPASy TrEMBL
Match: A0A0A0KST2 (Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.0e-79
Identity = 157/178 (88.20%), Postives = 166/178 (93.26%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKS +S+DSP GST +P+LGS       RTQIAELEQVF KFDVNGDGKI SSEL
Sbjct: 6   LFSRKKKSHSSTDSPLGSTTLPILGS-------RTQIAELEQVFNKFDVNGDGKICSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEE+QNMIKEVDADGDG+IDLDEFIELNTKGVDS+EVLENLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSDEVLENLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           DIDGNGSITAEELHEVLKSLGD+CSLADCRKMITGVD+NGDGMISFDEFKVMMMSGSR
Sbjct: 126 DIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 176

BLAST of MS009048 vs. ExPASy TrEMBL
Match: A0A5A7TS48 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3042G00010 PE=4 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 6.3e-78
Identity = 155/178 (87.08%), Postives = 162/178 (91.01%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSEL 60
           LFSRKKKS  S+DSP GST +P+ GS       RTQIAELEQVF KFDVNGDGKI SSEL
Sbjct: 6   LFSRKKKSHPSTDSPLGSTTLPIFGS-------RTQIAELEQVFNKFDVNGDGKICSSEL 65

Query: 61  GSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVY 120
           GSIMGSLGQPATEEE+ NMIKEVDADGDG+IDLDEFIELNTKGVDS EVLENLKDAFSVY
Sbjct: 66  GSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVY 125

Query: 121 DIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSR 179
           DIDGNGSITAEELHEVLKSLGD+CSLADCRKMITGVD+NGDGMISFDEFKVMMMSGSR
Sbjct: 126 DIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVMMMSGSR 176

BLAST of MS009048 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 217.2 bits (552), Expect = 1.0e-56
Identity = 111/182 (60.99%), Postives = 141/182 (77.47%), Query Frame = 0

Query: 1   LFSRKKKSDASSDSPPGSTAVPVLGSRTP----SFNCRTQIAELEQVFKKFDVNGDGKIS 60
           +F++ + S+  S S  G      +G+ +P    + + +T+I ELE VFKKFDVNGDGKIS
Sbjct: 1   MFNKNQGSNGGSSSNVG------IGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKIS 60

Query: 61  SSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDA 120
           S ELG+IM SLG    EEE++  I E+D  GDG+I+ +EF+ELNTKG+D  +VLENLKDA
Sbjct: 61  SKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDA 120

Query: 121 FSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSG 179
           FSVYDIDGNGSI+AEELHEVL+SLGDECS+A+CRKMI GVD++GDG I F+EFK+MM  G
Sbjct: 121 FSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMG 176

BLAST of MS009048 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 151.0 bits (380), Expect = 9.1e-37
Identity = 72/149 (48.32%), Postives = 109/149 (73.15%), Query Frame = 0

Query: 32  NCRTQIAELEQVFKKFDVNGDGKISSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFI 91
           +C   + ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M+K+ D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 92  DLDEFIELNTKGV----DSEEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLA 151
           DLDEF+ L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG++CS+ 
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 152 DCRKMITGVDRNGDGMISFDEFKVMMMSG 177
           DC+KMI+ VD +GDG ++FDEFK MM +G
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMSNG 158

BLAST of MS009048 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-36
Identity = 72/151 (47.68%), Postives = 107/151 (70.86%), Query Frame = 0

Query: 26  SRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSELGSIMGSLGQPATEEEIQNMIKEVDA 85
           S+  S NC   + ++++VF++FD N DGKIS  EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 86  DGDGFIDLDEFIEL--NTKGVDSEEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDE 145
           DG+GFIDLDEF+ L   +    +   + +LK+AF +YD+D NG I+A ELH V+K+LG++
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 146 CSLADCRKMITGVDRNGDGMISFDEFKVMMM 175
           CS+ DC++MI  VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

BLAST of MS009048 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.4 bits (337), Expect = 8.8e-32
Identity = 72/160 (45.00%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 18  STAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSELGSIMGSLGQPATEEEIQ 77
           ++A P     TP+        EL++VF +FD NGDGKIS  ELG +  ++G   TE E+ 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 78  NMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVYDIDGNGSITAEELHEVL 137
            +++EVD D DG+I+LDEF  L      + E+    +DAF +YD D NG I+A ELH+VL
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVL 121

Query: 138 KSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGS 178
             LG  CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 122 NRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

BLAST of MS009048 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.4 bits (337), Expect = 8.8e-32
Identity = 72/160 (45.00%), Postives = 100/160 (62.50%), Query Frame = 0

Query: 18  STAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGDGKISSSELGSIMGSLGQPATEEEIQ 77
           ++A P     TP+        EL++VF +FD NGDGKIS  ELG +  ++G   TE E+ 
Sbjct: 2   ASANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELN 61

Query: 78  NMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLENLKDAFSVYDIDGNGSITAEELHEVL 137
            +++EVD D DG+I+LDEF  L      + E+    +DAF +YD D NG I+A ELH+VL
Sbjct: 62  RVLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVL 121

Query: 138 KSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGS 178
             LG  CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 122 NRLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146331.11.9e-92100.00probable calcium-binding protein CML25 [Momordica charantia][more]
XP_023513887.19.3e-8490.45probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo][more]
XP_022963580.19.3e-8490.45probable calcium-binding protein CML25 [Cucurbita moschata][more]
XP_022990613.19.3e-8490.45probable calcium-binding protein CML25 [Cucurbita maxima][more]
KAG6601861.13.5e-8389.89putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s... [more]
Match NameE-valueIdentityDescription
Q9FYK21.5e-5560.99Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ... [more]
Q8RZB54.6e-4152.54Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
P250701.3e-3548.32Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2[more]
Q9C8Y12.2e-3547.68Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ... [more]
O649431.9e-3451.80Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CZ209.1e-93100.00probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1JTS94.5e-8490.45probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874... [more]
A0A6J1HID94.5e-8490.45probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A0A0KST22.0e-7988.20Polcalcin Jun o OS=Cucumis sativus OX=3659 GN=Csa_5G561760 PE=4 SV=1[more]
A0A5A7TS486.3e-7887.08Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT1G24620.11.0e-5660.99EF hand calcium-binding protein family [more]
AT5G37770.19.1e-3748.32EF hand calcium-binding protein family [more]
AT1G66400.11.6e-3647.68calmodulin like 23 [more]
AT1G18210.18.8e-3245.00Calcium-binding EF-hand family protein [more]
AT1G18210.28.8e-3245.00Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 112..140
e-value: 1.3E-5
score: 34.7
coord: 39..67
e-value: 8.8E-7
score: 38.5
coord: 148..176
e-value: 2.9E-5
score: 33.5
coord: 75..103
e-value: 1.6E-4
score: 31.0
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 111..173
e-value: 3.4E-12
score: 46.6
coord: 39..98
e-value: 5.4E-14
score: 52.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 108..143
score: 15.461849
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 71..106
score: 13.955498
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 35..70
score: 15.991861
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 144..178
score: 11.054376
IPR002048EF-hand domainCDDcd00051EFhcoord: 39..99
e-value: 2.52278E-21
score: 80.2845
IPR002048EF-hand domainCDDcd00051EFhcoord: 113..174
e-value: 2.25566E-19
score: 75.2769
NoneNo IPR availableGENE3D1.10.238.10coord: 103..178
e-value: 4.3E-25
score: 89.8
coord: 27..102
e-value: 3.0E-25
score: 90.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 12..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 23..177
NoneNo IPR availablePANTHERPTHR23050:SF382CALCIUM-BINDING PROTEIN CML25-RELATEDcoord: 23..177
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 121..133
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 48..60
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 84..96
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 157..169
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 31..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009048.1MS009048.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding