MS008848 (gene) Bitter gourd (TR) v1

Overview


Name	MS008848
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Maf-like protein
Location	scaffold4: 4226549 .. 4229318 (+)
RNA-Seq Expression	MS008848
Synteny	MS008848

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGTGTGCTTTTTGGCAGATAATTTTGGGATCATCTTCAGTGGCGCGTAGGAAAATATTATCTGAGATGGGTTATGAATTCACCATTATGGTATTCTCTACCTCGATGTACTTAAATGATCTGGCAACTCTCCCTTCTCACAATGTTTACCTATTGTTTCTCTCAAAATGAAGACTGCAGATATTGATGAGAAAGCCATACGCAAAGAAAAGCCTGAAGAGTTGGTTGTGGCTCTTGCAGAAGCAAAGGTTACGTATCTTATGATACACTTGTGCTGCAACGCATTCTGTGACTGAAATATTACTCCACATGTTGTTGGTACCGTTTTATGTAAATTGCTAGTGATAGAAATGAGCACTGTTGCCAATAGAAGATTTTGGATTTAATTTTGTCATTACTTCCATGCTAACATGACAAAATTAGAGAGAAATTAGATTTTAGAAACTTCTATTTGCCACATAATATTTGTGACCTTCTAAAACTGTCTTACAGTTGATACATCATACAATGACTGCATAATAGGGATGTTATTCTACAACGATGAAGCAAATAGTGTAAAAAAACGAAAGATTTGACTAACCCTTTCAATCTAGGGTCATGGATTTCAAAGCCCAACGAGCCAGGAATAATGGACTCACATTTTCTCTTTTGCATCTGTTTTTGTTTTCTGACTTCCTTTTAAAATCTCTATATAGCATAAACTGGCTTGTGATTTTTTTTTGCTCCAGCATCTTGTATTAATTAGGTATGAATTTCATCTCAGGCTGATGCCATCATATCAAATTTACATAAAATTCATACACATGAAAAGGAAGCAGAACCAACAATTTTGATTGCTGCTGACACAGTATGTTTTCCCATGTTAGTGTTAGACGATGTTCTCACTGTTGAACAATAGGGTGCATAATGGCTTGTATCACTTGAGGCCATTTATGAATTGAGGATACAACCACAACGCTCATTTTAAGATAGGACGTGTTTCGTTAGAATTAATTCTCTCAATGTGCAACTTTTTGTTGCATTTTTTAAGATGTAACTGTTTCATTGTCAAAGCCTCTTCTTCCACTGTCATCCACAAATTCAGTAATGGATTTGGTTGTGGTAGTAGGTCACTCTGGCTTATTTCTTAGCTGATGGATATTTATTGTCAGGCAGAAGCTATCTTAGGAAGACTCTCCATTGATGATTATATCAAGGATGCACAGCCAACACTTTTGATTACTTCTGATCAAGTATATCTATCAAGTTCTTTGACATCCGTTCATTTTGATATGTTTTAAGTGATTTCTTAATTTTTCAACTTGAATCACATGAAAATGATTGTAAGCTCTACCTGACTCAGTAAGATAATAAAAGATTTGAAAGCCTGGTAGAAAGGTTGTCAATTTGATTAAGCAGCGATGCTTTCTAAACTGGCATGTTGAATCGTCCTAGTTTTCCAAACTGACTTTAGAATCTTAGACATTTCTAATATTTTAAATTATTTGCAGCTGACTAATACTACATATATATATTTTTAAATAAAAGATGATAACAAAAACTTGATTAGAATCATTTTCATATTTGACATCTATTTCTTTACTTGAATCATGCAGCCGATAATGGCAAATCCTTGATGGTTTTTCCTTGGTCTCTGTCCCCTTCACTAATAACCAGAGTAACTATCCTGACAATCCCATGTGAAATACCTGCCGCCACATGTTGCACCTGTTGTACTCATTGTACCCATTTACCTTGAGATAGATGCTATGGCAGTTTTGTTTTGTTATATTGTGTATTCTTCGACCCGTTGTGTGTCATATCCTCAAAACATAAATGGTCCTTTCGTGTATGCCATAAGGACACTTTATTGTTCTGTTTGTATTTTTAGATGCTTTCTTACTTCTATTGTTAATTGATGTGGAGGTGGTGATATATGAAGGTGTCATCAGAGAAAAACCAGACAGCAAAGAGGAAGCACGGCAATTTATGAAAGGTTTGTTTATATAATAGTAATGCACTCCCATTTTTTTTCCATTTCTTAAGTTTTGTTCGCTAAAAAATTATATTGCAGATTATTCAGGAGGCCATGCTGCCACTCTTGGATCCGTACTTGTTACAAACCTTAAAACAGGATTTAGGAAAGGGGAATGGGATCGAGTAGAGGTATATATCTCTATTTTTATTTTTCTATCGGTGATCTTTTTATTTCTCGTTTAGTTGTTCATGCCTCTCTCAATCTTTTCCCTCTATAGTATTAGGGAAAAGACATTCACGTGGTTGAGACTGGTACCTTCCAATTGACTAAACCAATAAATGATATCCTAGTTTCGTAGACCTATTAACTAGTTCAACAAGGAACCGGCAGAATATAAGCTAGATACTGTTATTTTATATTGAGGGAAGGAGCTTCTTTTGCTGCTTCTGTCCGATATTGATTTTCTGATTCCTAGCAACAGAAAGATGACTTCTAGATGATTTTGGTTTTGTTTCACATCACACGTCTCTTTGTTTAATTATGGTATATCTGATATTAATGTGCGCTCTTACCTTGCAGATCTATTTCCATGAAATACCCGATGAAGTCATCGACAAGCTGGTAGAGTTTTTCACTTTATGATTGGTTGTTGGTCGTGTTTTCATTAGGAAAACAAAATATTATACGTTCTGTCATCTGATTTTCCCTTTTTTCTTCTATACATTTCAGGTCGAGGAGGGGATTGTGCTCAATGTTGCCGGAGGGCTAATAATCGAGCATCCTTTGATCTTACCCTATGTTAAAGAAGTGGTT

mRNA sequence

ACGTGTGCTTTTTGGCAGATAATTTTGGGATCATCTTCAGTGGCGCGTAGGAAAATATTATCTGAGATGGGTTATGAATTCACCATTATGACTGCAGATATTGATGAGAAAGCCATACGCAAAGAAAAGCCTGAAGAGTTGGTTGTGGCTCTTGCAGAAGCAAAGGCTGATGCCATCATATCAAATTTACATAAAATTCATACACATGAAAAGGAAGCAGAACCAACAATTTTGATTGCTGCTGACACAGCAGAAGCTATCTTAGGAAGACTCTCCATTGATGATTATATCAAGGATGCACAGCCAACACTTTTGATTACTTCTGATCAAGTGGTGATATATGAAGGTGTCATCAGAGAAAAACCAGACAGCAAAGAGGAAGCACGGCAATTTATGAAAGATTATTCAGGAGGCCATGCTGCCACTCTTGGATCCGTACTTGTTACAAACCTTAAAACAGGATTTAGGAAAGGGGAATGGGATCGAGTAGAGATCTATTTCCATGAAATACCCGATGAAGTCATCGACAAGCTGGTCGAGGAGGGGATTGTGCTCAATGTTGCCGGAGGGCTAATAATCGAGCATCCTTTGATCTTACCCTATGTTAAAGAAGTGGTT

Coding sequence (CDS)

ACGTGTGCTTTTTGGCAGATAATTTTGGGATCATCTTCAGTGGCGCGTAGGAAAATATTATCTGAGATGGGTTATGAATTCACCATTATGACTGCAGATATTGATGAGAAAGCCATACGCAAAGAAAAGCCTGAAGAGTTGGTTGTGGCTCTTGCAGAAGCAAAGGCTGATGCCATCATATCAAATTTACATAAAATTCATACACATGAAAAGGAAGCAGAACCAACAATTTTGATTGCTGCTGACACAGCAGAAGCTATCTTAGGAAGACTCTCCATTGATGATTATATCAAGGATGCACAGCCAACACTTTTGATTACTTCTGATCAAGTGGTGATATATGAAGGTGTCATCAGAGAAAAACCAGACAGCAAAGAGGAAGCACGGCAATTTATGAAAGATTATTCAGGAGGCCATGCTGCCACTCTTGGATCCGTACTTGTTACAAACCTTAAAACAGGATTTAGGAAAGGGGAATGGGATCGAGTAGAGATCTATTTCCATGAAATACCCGATGAAGTCATCGACAAGCTGGTCGAGGAGGGGATTGTGCTCAATGTTGCCGGAGGGCTAATAATCGAGCATCCTTTGATCTTACCCTATGTTAAAGAAGTGGTT

Protein sequence

TCAFWQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLHKIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDSKEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIVLNVAGGLIIEHPLILPYVKEVV

Homology

[Close]

BLAST of MS008848 vs. NCBI nr
Match: XP_022132591.1 (maf-like protein DDB_G0281937 isoform X1 [Momordica charantia])

HSP 1 Score: 384.4 bits (986), Expect = 6.1e-103
Identity = 198/202 (98.02%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           ++IILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH
Sbjct: 8   FKIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 67

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 124
           KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS
Sbjct: 68  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 127

Query: 125 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIV 184
           KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWD+VEIYFHEIPDEVIDKLVEEGIV
Sbjct: 128 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDQVEIYFHEIPDEVIDKLVEEGIV 187

Query: 185 LNVAGGLIIEHPLILPYVKEVV 207
           LNVAGGLIIEHPLI+PYVKEVV
Sbjct: 188 LNVAGGLIIEHPLIIPYVKEVV 209

[Close]

BLAST of MS008848 vs. NCBI nr
Match: XP_038905433.1 (7-methyl-GTP pyrophosphatase isoform X4 [Benincasa hispida])

HSP 1 Score: 361.7 bits (927), Expect = 4.2e-96
Identity = 187/202 (92.57%), Postives = 197/202 (97.52%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           ++IILGSSSVARRKILSEMGYEFTIM+ADIDEKAIRKE+PEELVVALAEAKADAIISNL 
Sbjct: 8   FKIILGSSSVARRKILSEMGYEFTIMSADIDEKAIRKERPEELVVALAEAKADAIISNLQ 67

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 124
           KIHTHEKEAEPTILIAADTAEAILGRLSIDD++KDA+PTLLITSDQVVIYEGVIREKP S
Sbjct: 68  KIHTHEKEAEPTILIAADTAEAILGRLSIDDFMKDAEPTLLITSDQVVIYEGVIREKPAS 127

Query: 125 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIV 184
           KEEARQFMKD+SGGHAATLGSV VTNLKTGFRKGEWDRVEI+F EIPDEVIDKLVEEG V
Sbjct: 128 KEEARQFMKDFSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFDEIPDEVIDKLVEEGSV 187

Query: 185 LNVAGGLIIEHPLILPYVKEVV 207
           LNVAGGLIIEHPLILP+VKEVV
Sbjct: 188 LNVAGGLIIEHPLILPHVKEVV 209

[Close]

BLAST of MS008848 vs. NCBI nr
Match: XP_038905430.1 (7-methyl-GTP pyrophosphatase isoform X1 [Benincasa hispida])

HSP 1 Score: 361.7 bits (927), Expect = 4.2e-96
Identity = 187/202 (92.57%), Postives = 197/202 (97.52%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           ++IILGSSSVARRKILSEMGYEFTIM+ADIDEKAIRKE+PEELVVALAEAKADAIISNL 
Sbjct: 8   FKIILGSSSVARRKILSEMGYEFTIMSADIDEKAIRKERPEELVVALAEAKADAIISNLQ 67

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 124
           KIHTHEKEAEPTILIAADTAEAILGRLSIDD++KDA+PTLLITSDQVVIYEGVIREKP S
Sbjct: 68  KIHTHEKEAEPTILIAADTAEAILGRLSIDDFMKDAEPTLLITSDQVVIYEGVIREKPAS 127

Query: 125 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIV 184
           KEEARQFMKD+SGGHAATLGSV VTNLKTGFRKGEWDRVEI+F EIPDEVIDKLVEEG V
Sbjct: 128 KEEARQFMKDFSGGHAATLGSVFVTNLKTGFRKGEWDRVEIFFDEIPDEVIDKLVEEGSV 187

Query: 185 LNVAGGLIIEHPLILPYVKEVV 207
           LNVAGGLIIEHPLILP+VKEVV
Sbjct: 188 LNVAGGLIIEHPLILPHVKEVV 209

[Close]

BLAST of MS008848 vs. NCBI nr
Match: XP_011652416.1 (7-methyl-GTP pyrophosphatase [Cucumis sativus])

HSP 1 Score: 358.6 bits (919), Expect = 3.6e-95
Identity = 185/206 (89.81%), Postives = 198/206 (96.12%), Query Frame = 0


Query: 1   TCAFWQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAII 60
           T + ++IILGSSSVARRKILSEMGYEFTIM+ADIDEKAIRKEKPEELVVALAEAKADAII
Sbjct: 4   TSSSFKIILGSSSVARRKILSEMGYEFTIMSADIDEKAIRKEKPEELVVALAEAKADAII 63

Query: 61  SNLHKIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIRE 120
           SNL  IHTHEKEAEPT+LIAADTA+AILGRLS DD++KDA+PTLLITSDQVVIYEGVIRE
Sbjct: 64  SNLQNIHTHEKEAEPTVLIAADTADAILGRLSTDDFMKDAEPTLLITSDQVVIYEGVIRE 123

Query: 121 KPDSKEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVE 180
           KP SKEEARQF+KDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEI+F+EIPDEVI+KLVE
Sbjct: 124 KPASKEEARQFLKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIFFNEIPDEVINKLVE 183

Query: 181 EGIVLNVAGGLIIEHPLILPYVKEVV 207
           EG VL VAGGLIIEHPLILPYVKEVV
Sbjct: 184 EGTVLYVAGGLIIEHPLILPYVKEVV 209

[Close]

BLAST of MS008848 vs. NCBI nr
Match: KAE8651328.1 (hypothetical protein Csa_000804 [Cucumis sativus])

HSP 1 Score: 358.6 bits (919), Expect = 3.6e-95
Identity = 185/206 (89.81%), Postives = 198/206 (96.12%), Query Frame = 0


Query: 1   TCAFWQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAII 60
           T + ++IILGSSSVARRKILSEMGYEFTIM+ADIDEKAIRKEKPEELVVALAEAKADAII
Sbjct: 4   TSSSFKIILGSSSVARRKILSEMGYEFTIMSADIDEKAIRKEKPEELVVALAEAKADAII 63

Query: 61  SNLHKIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIRE 120
           SNL  IHTHEKEAEPT+LIAADTA+AILGRLS DD++KDA+PTLLITSDQVVIYEGVIRE
Sbjct: 64  SNLQNIHTHEKEAEPTVLIAADTADAILGRLSTDDFMKDAEPTLLITSDQVVIYEGVIRE 123

Query: 121 KPDSKEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVE 180
           KP SKEEARQF+KDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEI+F+EIPDEVI+KLVE
Sbjct: 124 KPASKEEARQFLKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIFFNEIPDEVINKLVE 183

Query: 181 EGIVLNVAGGLIIEHPLILPYVKEVV 207
           EG VL VAGGLIIEHPLILPYVKEVV
Sbjct: 184 EGTVLYVAGGLIIEHPLILPYVKEVV 209

[Close]

BLAST of MS008848 vs. ExPASy Swiss-Prot
Match: Q54TC5 (7-methyl-GTP pyrophosphatase OS=Dictyostelium discoideum OX=44689 GN=DDB_G0281937 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 6.3e-26
Identity = 66/189 (34.92%), Postives = 111/189 (58.73%), Query Frame = 0


Query: 7   IILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLHKI 66
           +ILGSSS+ R+++L +MGY F  M+ DIDEKAIR   P+ L + ++ AKA A++  +   
Sbjct: 6   LILGSSSIWRKQVLIDMGYIFKTMSPDIDEKAIRDSDPKTLTLLISRAKAQALLKRI--- 65

Query: 67  HTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDSKE 126
               KE++  +                       + +++I SDQV+++ GVIREKP++++
Sbjct: 66  ----KESDDEL----------------------DKKSIMICSDQVIVHNGVIREKPETEQ 125

Query: 127 EARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIVLN 186
           + R++++ Y    A  + SV+V N++TG      D    +F +I DE IDKL+++G V++
Sbjct: 126 QCREYLQSYEFHPAVAVVSVVVVNIETGKIVEGTDIATQHFKKISDEFIDKLIKQGDVMH 165

Query: 187 VAGGLIIEH 196
            AGG  +EH
Sbjct: 186 CAGGFTVEH 165

[Close]

BLAST of MS008848 vs. ExPASy Swiss-Prot
Match: A6TQH7 (dTTP/UTP pyrophosphatase OS=Alkaliphilus metalliredigens (strain QYMF) OX=293826 GN=Amet_2288 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.1e-09
Identity = 46/189 (24.34%), Postives = 88/189 (46.56%), Query Frame = 0


Query: 6   QIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLHK 65
           ++IL S+S  R++IL  +  +F I+ +D+DE    K+ P ++V  LA  KA+ + + + +
Sbjct: 3   RLILASNSPRRKEILQNLHVKFDIIVSDVDEVFNEKDHPAKIVETLAYLKAEDVANRIDR 62

Query: 66  IHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDSK 125
                      I+I ADT                           +V+  G+I  KP +K
Sbjct: 63  ---------DAIIIGADT---------------------------IVVKNGII-GKPKNK 122

Query: 126 EEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIVL 185
           ++AR  ++  SG     +  ++V +  +G+   +    E+Y  +I DE I++ +  G  +
Sbjct: 123 QDARDILRTLSGDVHEVITGIVVLDTSSGYTVIDHVVTEVYMKKITDEEIERYIATGEPM 154

Query: 186 NVAGGLIIE 195
           + AG   I+
Sbjct: 183 DKAGAYGIQ 154

[Close]

BLAST of MS008848 vs. ExPASy Swiss-Prot
Match: Q5QZ35 (7-methyl-GTP pyrophosphatase OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM 15497 / L2-TR) OX=283942 GN=IL1346 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.0e-09
Identity = 50/174 (28.74%), Postives = 80/174 (45.98%), Query Frame = 0


Query: 7   IILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLHKI 66
           +ILGS S  RR+IL  +   + ++  DIDE AI  E P++LV  LAEAKA A+       
Sbjct: 5   LILGSGSKYRREILDRLHLNYDVVKPDIDESAISSESPQQLVGRLAEAKARAV------- 64

Query: 67  HTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDSKE 126
              EK                  R++ D+        ++I SDQV + +G I  KP ++E
Sbjct: 65  ---EK------------------RMTYDN-------AIIIGSDQVAVCDGNILGKPGNRE 124

Query: 127 EARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVE 181
            A + +  + G        + V N +    +   +  E+ F ++  E I++ VE
Sbjct: 125 NAVRQLSSFIGKTVTFYTGLAVFNTEAQQCEVRVEPFEVEFRQLTAEEIERYVE 143

[Close]

BLAST of MS008848 vs. ExPASy Swiss-Prot
Match: Q9X1P2 (dTTP/UTP pyrophosphatase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_1556 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 4.5e-08
Identity = 55/189 (29.10%), Postives = 85/189 (44.97%), Query Frame = 0


Query: 6   QIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLHK 65
           +IIL SSS  RR+++  +G EF +   D++E+ +  E PEE V  L+  KA+ +      
Sbjct: 2   RIILASSSPRRRQLMELLGIEFEVEKPDVEEEFL--ESPEETVRELSLRKAEWVF----- 61

Query: 66  IHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDSK 125
               +K  E  IL+                          I SD VV+ +G I  KP+S 
Sbjct: 62  ----KKRKEEEILV--------------------------IGSDTVVVLDGNILGKPESL 121

Query: 126 EEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIVL 185
           EEA+  +K  SG        V   + +T  +       ++ F E+P+ VID  VE+   L
Sbjct: 122 EEAKGMLKKLSGKWHVVYTGVAFVSSET--KDVIVSSTKVRFRELPESVIDYYVEKYRPL 151

Query: 186 NVAGGLIIE 195
           + AG   I+
Sbjct: 182 DKAGAYGIQ 151

[Close]

BLAST of MS008848 vs. ExPASy Swiss-Prot
Match: A5IM27 (dTTP/UTP pyrophosphatase OS=Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995 / JCM 10881 / RKU-1) OX=390874 GN=Tpet_1236 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 4.5e-08
Identity = 55/189 (29.10%), Postives = 85/189 (44.97%), Query Frame = 0


Query: 6   QIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLHK 65
           +IIL SSS  RR+++  +G EF +   D++E+ +  E PEE V  L+  KA+ +      
Sbjct: 2   RIILASSSPRRRQLMELLGIEFEVEKPDVEEEFL--ESPEETVRELSLRKAEWVF----- 61

Query: 66  IHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDSK 125
               +K  E  IL+                          I SD VV+ +G I  KP+S 
Sbjct: 62  ----KKRKEEEILV--------------------------IGSDTVVVLDGNILGKPESL 121

Query: 126 EEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIVL 185
           EEA+  +K  SG        V   + +T  +       ++ F E+P+ VID  VE+   L
Sbjct: 122 EEAKGMLKKLSGEWHVVYTGVAFVSSET--KDVIVSSTKVRFRELPESVIDYYVEKYRPL 151

Query: 186 NVAGGLIIE 195
           + AG   I+
Sbjct: 182 DKAGAYGIQ 151

[Close]

BLAST of MS008848 vs. ExPASy TrEMBL
Match: A0A6J1BSP3 (maf-like protein DDB_G0281937 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005415 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.0e-103
Identity = 198/202 (98.02%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           ++IILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH
Sbjct: 8   FKIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 67

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 124
           KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS
Sbjct: 68  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 127

Query: 125 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIV 184
           KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWD+VEIYFHEIPDEVIDKLVEEGIV
Sbjct: 128 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDQVEIYFHEIPDEVIDKLVEEGIV 187

Query: 185 LNVAGGLIIEHPLILPYVKEVV 207
           LNVAGGLIIEHPLI+PYVKEVV
Sbjct: 188 LNVAGGLIIEHPLIIPYVKEVV 209

[Close]

BLAST of MS008848 vs. ExPASy TrEMBL
Match: A0A1S3CRP6 (maf-like protein DDB_G0281937 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503944 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.3e-94
Identity = 184/206 (89.32%), Postives = 196/206 (95.15%), Query Frame = 0


Query: 1   TCAFWQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAII 60
           T + ++IILGSSSVARRKILSEMGYEFTIM+ADIDEKAIRKEKPEELVVALAEAKADAII
Sbjct: 4   TSSSFKIILGSSSVARRKILSEMGYEFTIMSADIDEKAIRKEKPEELVVALAEAKADAII 63

Query: 61  SNLHKIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIRE 120
           SNL  I THEKEAE T+LIAADTA+AILGRLS DD++KDA+PTLLITSDQVVIYEGVIRE
Sbjct: 64  SNLQNIDTHEKEAEQTVLIAADTADAILGRLSTDDFMKDAEPTLLITSDQVVIYEGVIRE 123

Query: 121 KPDSKEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVE 180
           KP SKEEARQF+KDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEI+F+EIPDEVIDKLVE
Sbjct: 124 KPASKEEARQFLKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIFFNEIPDEVIDKLVE 183

Query: 181 EGIVLNVAGGLIIEHPLILPYVKEVV 207
           EG VL VAGGLIIEHPLILPYVKEVV
Sbjct: 184 EGTVLYVAGGLIIEHPLILPYVKEVV 209

[Close]

BLAST of MS008848 vs. ExPASy TrEMBL
Match: A0A5A7TDU8 (Maf-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00450 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 5.6e-94
Identity = 183/206 (88.83%), Postives = 196/206 (95.15%), Query Frame = 0


Query: 1   TCAFWQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAII 60
           T + ++IILGSSSVARRKILSEMGYEFTIM+ADIDEKAIRKEKPEELVVALAEAKADAII
Sbjct: 4   TSSSFKIILGSSSVARRKILSEMGYEFTIMSADIDEKAIRKEKPEELVVALAEAKADAII 63

Query: 61  SNLHKIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIRE 120
           SNL  I THEKEAE T+LIAADTA+AILGRLS DD++KDA+PTLLITSDQVVIYEGVIRE
Sbjct: 64  SNLQNIDTHEKEAEQTVLIAADTADAILGRLSTDDFMKDAEPTLLITSDQVVIYEGVIRE 123

Query: 121 KPDSKEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVE 180
           KP SKEEARQF+KDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEI+F+EIPDEVIDKLVE
Sbjct: 124 KPASKEEARQFLKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIFFNEIPDEVIDKLVE 183

Query: 181 EGIVLNVAGGLIIEHPLILPYVKEVV 207
           EG VL VAGGLIIEHPLILPYVKEV+
Sbjct: 184 EGTVLYVAGGLIIEHPLILPYVKEVI 209

[Close]

BLAST of MS008848 vs. ExPASy TrEMBL
Match: A0A2I4EQJ8 (7-methyl-GTP pyrophosphatase isoform X1 OS=Juglans regia OX=51240 GN=LOC108991743 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.6e-85
Identity = 167/201 (83.08%), Postives = 185/201 (92.04%), Query Frame = 0


Query: 6   QIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLHK 65
           QIILGSSS+ARR IL+EMGYEFTIMTADIDEK IRK+KPE+LV+ALAEAKADAIIS L  
Sbjct: 13  QIILGSSSIARRTILAEMGYEFTIMTADIDEKCIRKDKPEDLVMALAEAKADAIISKLQI 72

Query: 66  IHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDSK 125
           I+  EK+ E TILIAADTAEAIL R  I DYIKDA+PTLLIT DQVV+YEGV+REKP SK
Sbjct: 73  INHQEKDVEQTILIAADTAEAILQR-PIGDYIKDAEPTLLITCDQVVVYEGVVREKPSSK 132

Query: 126 EEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIVL 185
           EEARQ++KDYSG HAAT+GSVLVTNLKTGFRKGEWDRVEIYFHEIPDE+I+KL+EEGIVL
Sbjct: 133 EEARQYLKDYSGSHAATVGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEIIEKLIEEGIVL 192

Query: 186 NVAGGLIIEHPLILPYVKEVV 207
           NVAGGLIIEHPLILP+VK+VV
Sbjct: 193 NVAGGLIIEHPLILPFVKQVV 212

[Close]

BLAST of MS008848 vs. ExPASy TrEMBL
Match: A0A2I4EQJ9 (7-methyl-GTP pyrophosphatase isoform X2 OS=Juglans regia OX=51240 GN=LOC108991743 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.6e-85
Identity = 166/202 (82.18%), Postives = 186/202 (92.08%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           ++IILGSSS+ARR IL+EMGYEFTIMTADIDEK IRK+KPE+LV+ALAEAKADAIIS L 
Sbjct: 8   FKIILGSSSIARRTILAEMGYEFTIMTADIDEKCIRKDKPEDLVMALAEAKADAIISKLQ 67

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 124
            I+  EK+ E TILIAADTAEAIL R  I DYIKDA+PTLLIT DQVV+YEGV+REKP S
Sbjct: 68  IINHQEKDVEQTILIAADTAEAILQR-PIGDYIKDAEPTLLITCDQVVVYEGVVREKPSS 127

Query: 125 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIV 184
           KEEARQ++KDYSG HAAT+GSVLVTNLKTGFRKGEWDRVEIYFHEIPDE+I+KL+EEGIV
Sbjct: 128 KEEARQYLKDYSGSHAATVGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEIIEKLIEEGIV 187

Query: 185 LNVAGGLIIEHPLILPYVKEVV 207
           LNVAGGLIIEHPLILP+VK+VV
Sbjct: 188 LNVAGGLIIEHPLILPFVKQVV 208

[Close]

BLAST of MS008848 vs. TAIR 10
Match: AT5G42770.2 (Maf-like protein )

HSP 1 Score: 261.2 bits (666), Expect = 7.3e-70
Identity = 135/202 (66.83%), Postives = 170/202 (84.16%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           +++ILGSSS+ARRKIL++MGY+FT+M+ADIDEK+IRKEKPEELV+ALAEAKADAI+S L 
Sbjct: 8   FKLILGSSSIARRKILTDMGYQFTLMSADIDEKSIRKEKPEELVLALAEAKADAIVSKL- 67

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 124
           +I   E E +P +LIA+DTAEAI+ R+   + I++ + TLLIT DQVV+YE  +REKP S
Sbjct: 68  QISECEDEEQPRVLIASDTAEAIMQRIPDGENIEEDKSTLLITCDQVVVYEDAVREKPSS 127

Query: 125 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIV 184
            EEAR++++ YS GH AT+ SV VTNLKTG RKG  DRVEIYF+EIP+E I+KL+EEG+V
Sbjct: 128 VEEAREYIRGYSKGHTATVSSVAVTNLKTGVRKGGVDRVEIYFNEIPEETIEKLIEEGMV 187

Query: 185 LNVAGGLIIEHPLILPYVKEVV 207
           L VAG L+IEHPLILP VKEVV
Sbjct: 188 LKVAGALLIEHPLILPCVKEVV 208

[Close]

BLAST of MS008848 vs. TAIR 10
Match: AT5G42770.1 (Maf-like protein )

HSP 1 Score: 223.8 bits (569), Expect = 1.3e-58
Identity = 120/202 (59.41%), Postives = 150/202 (74.26%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           +++ILGSSS+ARRKIL++MGY+FT+M+ADIDEK+IRKEKPEELV+ALAEAK         
Sbjct: 8   FKLILGSSSIARRKILTDMGYQFTLMSADIDEKSIRKEKPEELVLALAEAK--------- 67

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 124
                              AEAI+ R+   + I++ + TLLIT DQVV+YE  +REKP S
Sbjct: 68  -------------------AEAIMQRIPDGENIEEDKSTLLITCDQVVVYEDAVREKPSS 127

Query: 125 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIV 184
            EEAR++++ YS GH AT+ SV VTNLKTG RKG  DRVEIYF+EIP+E I+KL+EEG+V
Sbjct: 128 VEEAREYIRGYSKGHTATVSSVAVTNLKTGVRKGGVDRVEIYFNEIPEETIEKLIEEGMV 181

Query: 185 LNVAGGLIIEHPLILPYVKEVV 207
           L VAG L+IEHPLILP VKEVV
Sbjct: 188 LKVAGALLIEHPLILPCVKEVV 181

[Close]

BLAST of MS008848 vs. TAIR 10
Match: AT5G66550.1 (Maf-like protein )

HSP 1 Score: 220.7 bits (561), Expect = 1.1e-57
Identity = 113/202 (55.94%), Postives = 144/202 (71.29%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           +++ILGS S+AR++IL+EMGY++TI+TADIDEKAIR EKPE+LVVALAEAKA+ IIS L 
Sbjct: 10  FKLILGSQSMARKRILAEMGYDYTIVTADIDEKAIRTEKPEDLVVALAEAKANEIISKLG 69

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIREKPDS 124
                                          + KD QPTLLIT+D VV+Y+GVIREKP +
Sbjct: 70  ---------------------------GESQFAKDPQPTLLITADTVVVYKGVIREKPTT 129

Query: 125 KEEARQFMKDYSGGHAATLGSVLVTNLKTGFRKGEWDRVEIYFHEIPDEVIDKLVEEGIV 184
           KEEAR+F+K YSG H   +GSVLV NLKTG +KG WD+ E+YFHEIP++VID L+++ I 
Sbjct: 130 KEEAREFIKGYSGSHGGVVGSVLVRNLKTGVKKGGWDKAEVYFHEIPEQVIDGLIDDAIT 184

Query: 185 LNVAGGLIIEHPLILPYVKEVV 207
             VAGGL +EHPLI P++  VV
Sbjct: 190 YKVAGGLTLEHPLISPFIDSVV 184

[Close]

BLAST of MS008848 vs. TAIR 10
Match: AT2G25500.1 (Inosine triphosphate pyrophosphatase family protein )

HSP 1 Score: 104.4 bits (259), Expect = 1.1e-22
Identity = 59/116 (50.86%), Postives = 78/116 (67.24%), Query Frame = 0


Query: 5   WQIILGSSSVARRKILSEMGYEFTIMTADIDEKAIRKEKPEELVVALAEAKADAIISNLH 64
           +++ILGS S+AR++IL+EMGY+FTI+TADIDEKAIRKEKPE+LVV +AEAKA+ II  L 
Sbjct: 10  YKLILGSQSMARKQILAEMGYDFTIVTADIDEKAIRKEKPEDLVVTIAEAKANEIILKLG 69

Query: 65  KIHTHEKEAEPTILIAADTAEAILGRLSIDDYIKDAQPTLLITSDQVVIYEGVIRE 121
                                        + + +D+QPTLLIT+D VV+Y+GVIRE
Sbjct: 70  ---------------------------GENQFTQDSQPTLLITADTVVVYKGVIRE 98

The following BLAST results are available for this feature:

BLAST of MS008848 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132591.1	6.1e-103	98.02	maf-like protein DDB_G0281937 isoform X1 [Momordica charantia]	[more]
XP_038905433.1	4.2e-96	92.57	7-methyl-GTP pyrophosphatase isoform X4 [Benincasa hispida]	[more]
XP_038905430.1	4.2e-96	92.57	7-methyl-GTP pyrophosphatase isoform X1 [Benincasa hispida]	[more]
XP_011652416.1	3.6e-95	89.81	7-methyl-GTP pyrophosphatase [Cucumis sativus]	[more]
KAE8651328.1	3.6e-95	89.81	hypothetical protein Csa_000804 [Cucumis sativus]	[more]

BLAST of MS008848 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54TC5	6.3e-26	34.92	7-methyl-GTP pyrophosphatase OS=Dictyostelium discoideum OX=44689 GN=DDB_G028193...	[more]
A6TQH7	4.1e-09	24.34	dTTP/UTP pyrophosphatase OS=Alkaliphilus metalliredigens (strain QYMF) OX=293826...	[more]
Q5QZ35	7.0e-09	28.74	7-methyl-GTP pyrophosphatase OS=Idiomarina loihiensis (strain ATCC BAA-735 / DSM...	[more]
Q9X1P2	4.5e-08	29.10	dTTP/UTP pyrophosphatase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / ...	[more]
A5IM27	4.5e-08	29.10	dTTP/UTP pyrophosphatase OS=Thermotoga petrophila (strain ATCC BAA-488 / DSM 139...	[more]

BLAST of MS008848 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSP3	3.0e-103	98.02	maf-like protein DDB_G0281937 isoform X1 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A1S3CRP6	4.3e-94	89.32	maf-like protein DDB_G0281937 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503944...	[more]
A0A5A7TDU8	5.6e-94	88.83	Maf-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G004...	[more]
A0A2I4EQJ8	1.6e-85	83.08	7-methyl-GTP pyrophosphatase isoform X1 OS=Juglans regia OX=51240 GN=LOC10899174...	[more]
A0A2I4EQJ9	3.6e-85	82.18	7-methyl-GTP pyrophosphatase isoform X2 OS=Juglans regia OX=51240 GN=LOC10899174...	[more]

BLAST of MS008848 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42770.2	7.3e-70	66.83	Maf-like protein	[more]
AT5G42770.1	1.3e-58	59.41	Maf-like protein	[more]
AT5G66550.1	1.1e-57	55.94	Maf-like protein	[more]
AT2G25500.1	1.1e-22	50.86	Inosine triphosphate pyrophosphatase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029001	Inosine triphosphate pyrophosphatase-like	GENE3D	3.90.950.10		coord: 3..206 e-value: 1.1E-49 score: 170.6
IPR029001	Inosine triphosphate pyrophosphatase-like	SUPERFAMILY	52972	ITPase-like	coord: 5..198
IPR003697	Nucleoside triphosphate pyrophosphatase Maf-like protein	PFAM	PF02545	Maf	coord: 6..205 e-value: 1.5E-40 score: 138.9
IPR003697	Nucleoside triphosphate pyrophosphatase Maf-like protein	PIRSF	PIRSF006305	Maf	coord: 2..93 e-value: 8.6E-15 score: 52.5 coord: 84..205 e-value: 1.6E-17 score: 61.4
IPR003697	Nucleoside triphosphate pyrophosphatase Maf-like protein	PANTHER	PTHR43213	BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED	coord: 5..83
IPR003697	Nucleoside triphosphate pyrophosphatase Maf-like protein	PANTHER	PTHR43213	BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED	coord: 84..206
None	No IPR available	PANTHER	PTHR43213:SF12	MAF-LIKE PROTEIN	coord: 84..206
None	No IPR available	PANTHER	PTHR43213:SF12	MAF-LIKE PROTEIN	coord: 5..83

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008848.1	MS008848.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0047429	nucleoside-triphosphate diphosphatase activity