MS008826 (gene) Bitter gourd (TR) v1

Overview


Name	MS008826
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	EF hand calcium-binding protein family
Location	scaffold4: 4057131 .. 4057673 (-)
RNA-Seq Expression	MS008826
Synteny	MS008826

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCCAATTCCAACTGTTTCCATTGCCTTCAAATGGTTTTCCAAATCCAATTTCAAGCTCAACTTCCCTCGCCTTCTCTTCAACCCCAAACCCACCTCCTCTAATCCTCCTCCACCACCCCTCAAGGCGGCGCCACCGCTCGCCGAAGTCGCCTTTCACCACCTCGACGCCGATGGAGATGGCAAAATCTCATGCGACGAACTCCGATCCTACTTTGCATCCATCGGCGAGTGTATAACACGCGACGATGTTCGTAGAGTGATTCGAGACTTCGATGGCAACGGCGACGAGCTACTGGAGCTTCAAGACTTTGTTCGCCTAATGGAGGTCCAGGAGGAGGAGGAGGGAGAGGATTTGAGAAGGGCTTTTGAAATGTTTGAAGTTGAAAAGGGGTGCGGGTTCATCGGACCGGAGGGATTACAGAAGATGTTTCGACGGTTGGGGTATGTGAAATCGAGCGACGAGTGCATGGCAATGATCGACGTGTTTGACATTAATGGTGACGGTGTTATCGATTATCGTGAATTTCTCAGAATGATGGCA

mRNA sequence

ATTCCAATTCCAACTGTTTCCATTGCCTTCAAATGGTTTTCCAAATCCAATTTCAAGCTCAACTTCCCTCGCCTTCTCTTCAACCCCAAACCCACCTCCTCTAATCCTCCTCCACCACCCCTCAAGGCGGCGCCACCGCTCGCCGAAGTCGCCTTTCACCACCTCGACGCCGATGGAGATGGCAAAATCTCATGCGACGAACTCCGATCCTACTTTGCATCCATCGGCGAGTGTATAACACGCGACGATGTTCGTAGAGTGATTCGAGACTTCGATGGCAACGGCGACGAGCTACTGGAGCTTCAAGACTTTGTTCGCCTAATGGAGGTCCAGGAGGAGGAGGAGGGAGAGGATTTGAGAAGGGCTTTTGAAATGTTTGAAGTTGAAAAGGGGTGCGGGTTCATCGGACCGGAGGGATTACAGAAGATGTTTCGACGGTTGGGGTATGTGAAATCGAGCGACGAGTGCATGGCAATGATCGACGTGTTTGACATTAATGGTGACGGTGTTATCGATTATCGTGAATTTCTCAGAATGATGGCA

Coding sequence (CDS)

ATTCCAATTCCAACTGTTTCCATTGCCTTCAAATGGTTTTCCAAATCCAATTTCAAGCTCAACTTCCCTCGCCTTCTCTTCAACCCCAAACCCACCTCCTCTAATCCTCCTCCACCACCCCTCAAGGCGGCGCCACCGCTCGCCGAAGTCGCCTTTCACCACCTCGACGCCGATGGAGATGGCAAAATCTCATGCGACGAACTCCGATCCTACTTTGCATCCATCGGCGAGTGTATAACACGCGACGATGTTCGTAGAGTGATTCGAGACTTCGATGGCAACGGCGACGAGCTACTGGAGCTTCAAGACTTTGTTCGCCTAATGGAGGTCCAGGAGGAGGAGGAGGGAGAGGATTTGAGAAGGGCTTTTGAAATGTTTGAAGTTGAAAAGGGGTGCGGGTTCATCGGACCGGAGGGATTACAGAAGATGTTTCGACGGTTGGGGTATGTGAAATCGAGCGACGAGTGCATGGCAATGATCGACGTGTTTGACATTAATGGTGACGGTGTTATCGATTATCGTGAATTTCTCAGAATGATGGCA

Protein sequence

IPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA

Homology

[Close]

BLAST of MS008826 vs. NCBI nr
Match: XP_022139654.1 (probable calcium-binding protein CML41 [Momordica charantia])

HSP 1 Score: 369.8 bits (948), Expect = 1.4e-98
Identity = 180/181 (99.45%), Postives = 180/181 (99.45%), Query Frame = 0


Query: 1   IPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGD 60
           IPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGD
Sbjct: 3   IPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGD 62

Query: 61  GKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLR 120
           GKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDF RLMEVQEEEEGEDLR
Sbjct: 63  GKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLR 122

Query: 121 RAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 180
           RAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM
Sbjct: 123 RAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 182

Query: 181 A 182
           A
Sbjct: 183 A 183

[Close]

BLAST of MS008826 vs. NCBI nr
Match: XP_022974919.1 (probable calcium-binding protein CML41 [Cucurbita maxima])

HSP 1 Score: 267.3 bits (682), Expect = 9.5e-68
Identity = 134/178 (75.28%), Postives = 148/178 (83.15%), Query Frame = 0


Query: 6   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPP---LKAAPPLAEVAFHHLDADGDGK 65
           +S  FKWFS +NFKLNFPRL F  KPT SNPPPPP   L  APPLAE AF HLDADGDGK
Sbjct: 20  ISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 79

Query: 66  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRA 125
           IS DEL+SYFAS+GE +TRDD R +IR+FDG+GD LLEL DF RL++  EEEE EDL+ A
Sbjct: 80  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKMA 139

Query: 126 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 181
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM
Sbjct: 140 FEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 197

[Close]

BLAST of MS008826 vs. NCBI nr
Match: KAG6591338.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 265.0 bits (676), Expect = 4.7e-67
Identity = 133/178 (74.72%), Postives = 147/178 (82.58%), Query Frame = 0


Query: 6   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPP---LKAAPPLAEVAFHHLDADGDGK 65
           +S  FKWFS +N KLN PRL F  KPT SNPPPPP   L  APPLAE AF HLDADGDGK
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 65

Query: 66  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRA 125
           IS DEL+SYFAS+GE +TRDD R +IR+FDG+GD LLEL DF RL++  EEEE EDL+RA
Sbjct: 66  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKRA 125

Query: 126 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 181
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM
Sbjct: 126 FEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 183

[Close]

BLAST of MS008826 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 265.0 bits (676), Expect = 4.7e-67
Identity = 134/178 (75.28%), Postives = 147/178 (82.58%), Query Frame = 0


Query: 6   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPP---LKAAPPLAEVAFHHLDADGDGK 65
           VS  FKWFS +NF LNFPRL F  KPT SNPPPPP   L  APPLAE AF HLDADGDGK
Sbjct: 43  VSTPFKWFSNTNFNLNFPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 102

Query: 66  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRA 125
           IS DEL+SYFAS+GE +TRDD R +IR+FD +GD LLEL DF RL++  EEEE EDL+RA
Sbjct: 103 ISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKGGEEEEKEDLKRA 162

Query: 126 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 181
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM
Sbjct: 163 FEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 220

[Close]

BLAST of MS008826 vs. NCBI nr
Match: KAG7024217.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-66
Identity = 132/178 (74.16%), Postives = 147/178 (82.58%), Query Frame = 0


Query: 6   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPP---LKAAPPLAEVAFHHLDADGDGK 65
           +S  FKWFS +N KLN PRL F  KPT SNPPPPP   L  APPLAE AF HLDADGDGK
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 65

Query: 66  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRA 125
           IS DEL+SYFAS+GE +TRD+ R +IR+FDG+GD LLEL DF RL++  EEEE EDL+RA
Sbjct: 66  ISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKRA 125

Query: 126 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 181
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM
Sbjct: 126 FEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 183

[Close]

BLAST of MS008826 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-29
Identity = 81/194 (41.75%), Postives = 111/194 (57.22%), Query Frame = 0


Query: 7   SIAFKWFSKSNFKLN--FPRLLFNPKPTSSNPPPPPL------------KAAPPLAEVAF 66
           S +FKWFS    KLN  F     +PK  SS+    P             +A+       F
Sbjct: 10  SNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVF 69

Query: 67  HHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQE 126
            H D+DGDGKIS  ELR YF S+GE I+ +  +  I + D + D  L  +DFV LM  ++
Sbjct: 70  SHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRD 129

Query: 127 -----EEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDIN 181
                E +G+ +L+ AFEMFEVEKG G I P+GLQKM  +LG  ++  EC AMI  +DI+
Sbjct: 130 LYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDID 189

[Close]

BLAST of MS008826 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.2e-20
Identity = 63/144 (43.75%), Postives = 89/144 (61.81%), Query Frame = 0


Query: 43  AAPPLAEVAFHHLDADGDGKISCDELRSYF-ASIGECITRDDVRRVIRDFDGNGDELLEL 102
           A+P  +   F   D DGDG+IS  ELR     ++GE ++ ++  +++   D +GD LL  
Sbjct: 7   ASPCESSALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCE 66

Query: 103 QDFVRLM---EVQEEEE--GEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDEC 162
            +FVRL+   EV+EE+E  G  LR AF M+E+E G G I P  L++M RRLG  +  D+C
Sbjct: 67  AEFVRLVQAAEVEEEDERRGTGLREAFGMYEME-GEGCITPTSLRRMLRRLGSDQDIDDC 126

Query: 163 MAMIDVFDINGDGVIDYREFLRMM 181
            AMI  FD+NGDGV+ + EF  MM
Sbjct: 127 RAMICRFDLNGDGVLSFDEFKIMM 149

[Close]

BLAST of MS008826 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 7.7e-20
Identity = 48/133 (36.09%), Postives = 87/133 (65.41%), Query Frame = 0


Query: 49  EVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLM 108
           E  F ++DA+ DG+IS +EL+  F ++GE ++ ++    +R  D +GD +L+ ++F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 109 EVQEEEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDING 168
           +V +EEE + +L+ AF ++ + +G   I P  L+ M ++LG  +++D+C  MI  FD+N 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 169 DGVIDYREFLRMM 181
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of MS008826 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.1e-18
Identity = 59/164 (35.98%), Postives = 91/164 (55.49%), Query Frame = 0


Query: 27  FNPKPT------SSNPPPPPLKAAPPLAEVAFHHLDADGDGKISCDELRSYFASIGECIT 86
           ++P+P+      ++ P PP   AA    E  F   DA+GDG+IS  EL + F S+G  +T
Sbjct: 32  WSPRPSDLAAAAAAKPRPP---AAEDETERVFRKFDANGDGRISRAELAALFRSVGHAVT 91

Query: 87  RDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEG---EDLRRAFEMFEVEKGCGFIGP 146
            D+V R++++ D +GD  + L +F  +      +     EDLR AF +F+ + G G I P
Sbjct: 92  DDEVARMMQEADSDGDGYISLGEFAAISAPPPGDAAAAEEDLRHAFGVFDAD-GNGVITP 151

Query: 147 EGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMMA 182
             L ++ R +G   +  +C  MID  D NGDG+I++ EF  MMA
Sbjct: 152 AELARVLRGIGEAATVAQCRRMIDGVDRNGDGLINFEEFKLMMA 191

[Close]

BLAST of MS008826 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.5e-18
Identity = 47/133 (35.34%), Postives = 84/133 (63.16%), Query Frame = 0


Query: 52  FHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLME-- 111
           F   D +GDGKI+ +EL+ +F S+G  +  +++  +I   D NGD  +++ +F  L +  
Sbjct: 10  FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEM 69

Query: 112 VQEEEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYV--KSSDECMAMIDVFDING 171
           V+E+EE ED+R AF +F+ + G GFI  E L+ +   +G    ++ ++C  MI   D++G
Sbjct: 70  VEEKEEEEDMREAFRVFD-QNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDG 129

Query: 172 DGVIDYREFLRMM 181
           DG+++++EF +MM
Sbjct: 130 DGMVNFKEFKQMM 141

[Close]

BLAST of MS008826 vs. ExPASy TrEMBL
Match: A0A6J1CDM6 (probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC111010501 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 6.6e-99
Identity = 180/181 (99.45%), Postives = 180/181 (99.45%), Query Frame = 0


Query: 1   IPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGD 60
           IPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGD
Sbjct: 3   IPIPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGD 62

Query: 61  GKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLR 120
           GKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDF RLMEVQEEEEGEDLR
Sbjct: 63  GKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQEEEEGEDLR 122

Query: 121 RAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 180
           RAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM
Sbjct: 123 RAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 182

Query: 181 A 182
           A
Sbjct: 183 A 183

[Close]

BLAST of MS008826 vs. ExPASy TrEMBL
Match: A0A6J1IIY2 (probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC111473738 PE=4 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 4.6e-68
Identity = 134/178 (75.28%), Postives = 148/178 (83.15%), Query Frame = 0


Query: 6   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPP---LKAAPPLAEVAFHHLDADGDGK 65
           +S  FKWFS +NFKLNFPRL F  KPT SNPPPPP   L  APPLAE AF HLDADGDGK
Sbjct: 20  ISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDADGDGK 79

Query: 66  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRA 125
           IS DEL+SYFAS+GE +TRDD R +IR+FDG+GD LLEL DF RL++  EEEE EDL+ A
Sbjct: 80  ISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEEEEKEDLKMA 139

Query: 126 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 181
           FEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++GDGVIDYREFLRMM
Sbjct: 140 FEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREFLRMM 197

[Close]

BLAST of MS008826 vs. ExPASy TrEMBL
Match: A0A6J1F7Y7 (probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC111442970 PE=4 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.5e-66
Identity = 131/177 (74.01%), Postives = 147/177 (83.05%), Query Frame = 0


Query: 6   VSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPP--LKAAPPLAEVAFHHLDADGDGKI 65
           +S  FKWFS +N KLN PRL F  KPT SNPPPPP  L  APPLAE AF HLDADGDGKI
Sbjct: 6   ISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLTPAPPLAEAAFRHLDADGDGKI 65

Query: 66  SCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRAF 125
           S DEL+SYFAS+GE +TRDD R +IR+FDG+GD LLEL DF RL++  E+EE EDL+RAF
Sbjct: 66  SGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKGGEQEEKEDLKRAF 125

Query: 126 EMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 181
           EMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMI VFD++G+GVIDYREFLRMM
Sbjct: 126 EMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGVIDYREFLRMM 182

[Close]

BLAST of MS008826 vs. ExPASy TrEMBL
Match: A0A5A7UHX6 (Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27333G00010 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 3.2e-61
Identity = 125/178 (70.22%), Postives = 141/178 (79.21%), Query Frame = 0


Query: 3   IPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGDGK 62
           I T++I FKWF+K+NFKLNFP L    KP SS PP P     PPLAEVAF HLD DGDGK
Sbjct: 3   ISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTP-----PPLAEVAFRHLDIDGDGK 62

Query: 63  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRA 122
           IS DELRSYFASIGE +T D  + VI DFD +GDELLEL DF RL++ +EEE+ EDL+RA
Sbjct: 63  ISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEEDLKRA 122

Query: 123 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 181
           FEMFE EKGCGFIG  GLQKMF RLGYVKS ++CM MI VFD++GDGVIDY EFLRMM
Sbjct: 123 FEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMM 175

[Close]

BLAST of MS008826 vs. ExPASy TrEMBL
Match: A0A1S3CT00 (probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 3.2e-61
Identity = 125/178 (70.22%), Postives = 141/178 (79.21%), Query Frame = 0


Query: 3   IPTVSIAFKWFSKSNFKLNFPRLLFNPKPTSSNPPPPPLKAAPPLAEVAFHHLDADGDGK 62
           I T++I FKWF+K+NFKLNFP L    KP SS PP P     PPLAEVAF HLD DGDGK
Sbjct: 3   ISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTP-----PPLAEVAFRHLDIDGDGK 62

Query: 63  ISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQEEEEGEDLRRA 122
           IS DELRSYFASIGE +T D  + VI DFD +GDELLEL DF RL++ +EEE+ EDL+RA
Sbjct: 63  ISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQEEDLKRA 122

Query: 123 FEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVIDYREFLRMM 181
           FEMFE EKGCGFIG  GLQKMF RLGYVKS ++CM MI VFD++GDGVIDY EFLRMM
Sbjct: 123 FEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLRMM 175

[Close]

BLAST of MS008826 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 131.3 bits (329), Expect = 7.6e-31
Identity = 81/194 (41.75%), Postives = 111/194 (57.22%), Query Frame = 0


Query: 7   SIAFKWFSKSNFKLN--FPRLLFNPKPTSSNPPPPPL------------KAAPPLAEVAF 66
           S +FKWFS    KLN  F     +PK  SS+    P             +A+       F
Sbjct: 10  SNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVF 69

Query: 67  HHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLMEVQE 126
            H D+DGDGKIS  ELR YF S+GE I+ +  +  I + D + D  L  +DFV LM  ++
Sbjct: 70  SHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRD 129

Query: 127 -----EEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDIN 181
                E +G+ +L+ AFEMFEVEKG G I P+GLQKM  +LG  ++  EC AMI  +DI+
Sbjct: 130 LYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDID 189

[Close]

BLAST of MS008826 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 98.6 bits (244), Expect = 5.5e-21
Identity = 48/133 (36.09%), Postives = 87/133 (65.41%), Query Frame = 0


Query: 49  EVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLM 108
           E  F ++DA+ DG+IS +EL+  F ++GE ++ ++    +R  D +GD +L+ ++F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 109 EVQEEEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDING 168
           +V +EEE + +L+ AF ++ + +G   I P  L+ M ++LG  +++D+C  MI  FD+N 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 169 DGVIDYREFLRMM 181
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of MS008826 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 98.6 bits (244), Expect = 5.5e-21
Identity = 48/133 (36.09%), Postives = 87/133 (65.41%), Query Frame = 0


Query: 49  EVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLM 108
           E  F ++DA+ DG+IS +EL+  F ++GE ++ ++    +R  D +GD +L+ ++F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 109 EVQEEEEGE-DLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDING 168
           +V +EEE + +L+ AF ++ + +G   I P  L+ M ++LG  +++D+C  MI  FD+N 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 169 DGVIDYREFLRMM 181
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of MS008826 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 94.4 bits (233), Expect = 1.0e-19
Identity = 47/133 (35.34%), Postives = 84/133 (63.16%), Query Frame = 0


Query: 52  FHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLME-- 111
           F   D +GDGKI+ +EL+ +F S+G  +  +++  +I   D NGD  +++ +F  L +  
Sbjct: 10  FQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEM 69

Query: 112 VQEEEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYV--KSSDECMAMIDVFDING 171
           V+E+EE ED+R AF +F+ + G GFI  E L+ +   +G    ++ ++C  MI   D++G
Sbjct: 70  VEEKEEEEDMREAFRVFD-QNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDG 129

Query: 172 DGVIDYREFLRMM 181
           DG+++++EF +MM
Sbjct: 130 DGMVNFKEFKQMM 141

[Close]

BLAST of MS008826 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 91.7 bits (226), Expect = 6.7e-19
Identity = 47/136 (34.56%), Postives = 80/136 (58.82%), Query Frame = 0


Query: 49  EVAFHHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFVRLM 108
           E  F ++DA+ DG+IS +EL+  F ++GE ++ ++    ++  D +GD +L++ +F  L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 109 ----EVQEEEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFD 168
               E  EEE+   +  AF M+ +  G   I P  L+ M  +LG  +++D+C  MI  FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMY-IADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 169 INGDGVIDYREFLRMM 181
           +N DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

The following BLAST results are available for this feature:

BLAST of MS008826 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139654.1	1.4e-98	99.45	probable calcium-binding protein CML41 [Momordica charantia]	[more]
XP_022974919.1	9.5e-68	75.28	probable calcium-binding protein CML41 [Cucurbita maxima]	[more]
KAG6591338.1	4.7e-67	74.72	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023535419.1	4.7e-67	75.28	calmodulin-like protein 2 [Cucurbita pepo subsp. pepo]	[more]
KAG7024217.1	1.4e-66	74.16	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. a...	[more]

BLAST of MS008826 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L3R2	1.1e-29	41.75	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q8RYK0	1.2e-20	43.75	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE6	7.7e-20	36.09	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q6L4D4	1.1e-18	35.98	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SU00	1.5e-18	35.34	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]

BLAST of MS008826 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDM6	6.6e-99	99.45	probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IIY2	4.6e-68	75.28	probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC1114737...	[more]
A0A6J1F7Y7	1.5e-66	74.01	probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7UHX6	3.2e-61	70.22	Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CT00	3.2e-61	70.22	probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 P...	[more]

BLAST of MS008826 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	7.6e-31	41.75	calmodulin-like 41	[more]
AT1G76650.1	5.5e-21	36.09	calmodulin-like 38	[more]
AT1G76650.3	5.5e-21	36.09	calmodulin-like 38	[more]
AT4G12860.1	1.0e-19	35.34	EF hand calcium-binding protein family	[more]
AT1G76640.1	6.7e-19	34.56	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 83..111 e-value: 0.99 score: 17.8 coord: 47..75 e-value: 0.067 score: 22.3 coord: 155..181 e-value: 0.14 score: 21.3
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 133..180 e-value: 2.3E-9 score: 36.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 51..108 e-value: 5.5E-12 score: 45.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 9.854874
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 43..78 score: 12.114401
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 151..181 score: 14.06708
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 49..108 e-value: 4.15978E-11 score: 54.0909
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 118..181 e-value: 1.49946E-10 score: 52.5501
None	No IPR available	GENE3D	1.10.238.10		coord: 111..181 e-value: 7.5E-17 score: 63.1
None	No IPR available	GENE3D	1.10.238.10		coord: 32..110 e-value: 3.8E-16 score: 61.6
None	No IPR available	PANTHER	PTHR23050:SF393	CALMODULIN	coord: 36..181
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 36..181
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 164..176
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 56..68
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 51..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008826.1	MS008826.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding