MS008813 (gene) Bitter gourd (TR) v1

Overview


Name	MS008813
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ent-kaurene oxidase, chloroplastic
Location	scaffold4: 3854317 .. 3856538 (-)
RNA-Seq Expression	MS008813
Synteny	MS008813

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATCGCTTCTGATACTCTCTCCTCCATCCAGCAGCTCCTCGAGAGTCTTCACGCGCCGCCGTACGTGGCGGCGGCTGTTCTGGGTGGGGTGTCTCTGTTGCTTTCGGTGTTTGTGATCGGAGGTTGGAGGAAGAGACACCGGAATCTTCTGCCTCCGGTGCCGGGTAACGATAACGGCTTCTTCCCCATTTCTCTCTGTTTTTTGAAAATAGTGAAATGGGTGTTTGAATCTGCCTTCGATTATTAATGGGTTTTCTTTTTGTTGGGGATTTTGCAGCGATTCCGGGGCTGCCGGTGCTGGGGAATTTGCTGCAGTTGAAGGAGAAGAAGCCTCATAAGACGTTTGCTCGGTGGGCGGCGAAGTACGGCCCGGTCTATTCCATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCGCCGAAGTCGCCAAAGAGGTACCCATCACAGCCCTTTTCAGGCCAACTTTCGCCGGAAAACAAATGGTCTCGAGTTAAATTACAAGTTTGGTCGTCGAATCTTCGAAGATTAGTGAATTCATGTGTGAATTAATGTTTCAGGCAATGGTGACGAGATACTCGTCCATTTCCAGTAGAAAACTAAGCAAAGCCCTCACAATCTTGACTTCTGATAAATGCATGGTTGCCATGAGCGATTACAATGATTTTCACAAGATGGCTAAGAGATACATATTAGCAAATGTCTTGGGAGCTAATGCTCAGGTTGTTTTATATTTCTTTCTTTTTCCCTTCTTTTCATTTTGGATTATTCTGATTGTCTGCTCTGTTTCATTGTTCAGAAGAGACACAGACAGAGGAGAGACACCATGATAGAGAATATTTCCAGCCACTTGTTTGCTCATGCGAAGGATTCTCCATCGCAATCTGTTAATTTTCGAGAAATCTTCGAATTGGAACTCTTTCGGCTTGCACTTAAAGAAGTGAGTTGACTCTGTTGTTATGTTTAAACATGAAACTTGTGTAGTGTGGGAGCTAAAAAGAGAGAATTTTGTGATTGGCAGACACTGGGGAGCGATATCAAGTCGATATATGTCAGTGAGCTCGGTACTACTGCACTGAGAGAAGATCTGTTCAGGATTTTAGTGATTGATCCGATGGAGGGTGCGATCGAAGTCGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAACAAGGGTGTGGAGGATAAAATCAGGTCGATGGAGTATCGCAGGCGAGTGACGATGAAGAGCCTTATTGAGGAGTCGAAGAAGCGGATTGCAGCAGGGGAGGTAGTTCTTCATTCGCTTGAATAGAAAATAGGCCTATTTATCTACTCTGATGTAGAGAGTTGTTTGTTTTAACTTGGCTTGAATCTATTACAGGAAACGAATTCCTATGCTGAGTTCTTGTTATCTGAAGCAAAAACACTGACTGAGGATCAGATATCTATGTTGCTCTGGGAGACAATCATTGAAACCTCGGACACTACTTTGGTTGTGACAGAATGGGCTATGTATGAACTCGCTAAAGATCCTAAACGACAGGCAATTTCCGACCATCGCTAGCGTTTCGGTTGTTTTCTGTCTGTTCCAACTCTTAAAATCATCAAAACGAGTAATGATTCTTTGAAAACTTTTGACATTAACAGGACTATCTATATCAGCACATACTGAGTGTGTGTGGATCTGCTAAGCTTAAAGAAGAGAACCTGGCGCAGCTTCCATATCTGACTGCTGTGTTTCATGAAACTCTTCGGAAGCACAGTCCAGTCCCGATCGTCCCATTGCGATACGCTCATGAAGATACCCAGTTAGGAGGGTTCTTTGTTCCTGCAGGAAGTGAGGTAAGCAAACTCCCCTCCCCCGGGTGTAATTTGATGTGACGATATCCGATGATGTTTCGTTTTTTCGTTTTCAGATTGCCGTGAACATTTACGGCTGCAACATGGACAAGGAACACTGGGAAAGCCCCGAGGAATGGAAGCCAGAGAGGTTTCTTGATGAGAAATATGACCCAATGGATCTGCACAAAACAATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAAGCCTTGTAGCTTGCACAACCATTGGAAGGCTGGTTCAAGAGTTCGAGTGGAAGCTCGGGGGCGGGGAAGAAGACAAGGTCGACACTCTCGGCCTCACTGCTCGCAAGCTCCAACCGCTGCACGCAATCGTAAAGCCAAGAACCAAC

mRNA sequence

ATGGCGATCGCTTCTGATACTCTCTCCTCCATCCAGCAGCTCCTCGAGAGTCTTCACGCGCCGCCGTACGTGGCGGCGGCTGTTCTGGGTGGGGTGTCTCTGTTGCTTTCGGTGTTTGTGATCGGAGGTTGGAGGAAGAGACACCGGAATCTTCTGCCTCCGGTGCCGGCGATTCCGGGGCTGCCGGTGCTGGGGAATTTGCTGCAGTTGAAGGAGAAGAAGCCTCATAAGACGTTTGCTCGGTGGGCGGCGAAGTACGGCCCGGTCTATTCCATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCGCCGAAGTCGCCAAAGAGGCAATGGTGACGAGATACTCGTCCATTTCCAGTAGAAAACTAAGCAAAGCCCTCACAATCTTGACTTCTGATAAATGCATGGTTGCCATGAGCGATTACAATGATTTTCACAAGATGGCTAAGAGATACATATTAGCAAATGTCTTGGGAGCTAATGCTCAGAAGAGACACAGACAGAGGAGAGACACCATGATAGAGAATATTTCCAGCCACTTGTTTGCTCATGCGAAGGATTCTCCATCGCAATCTGTTAATTTTCGAGAAATCTTCGAATTGGAACTCTTTCGGCTTGCACTTAAAGAAACACTGGGGAGCGATATCAAGTCGATATATGTCAGTGAGCTCGGTACTACTGCACTGAGAGAAGATCTGTTCAGGATTTTAGTGATTGATCCGATGGAGGGTGCGATCGAAGTCGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAACAAGGGTGTGGAGGATAAAATCAGGTCGATGGAGTATCGCAGGCGAGTGACGATGAAGAGCCTTATTGAGGAGTCGAAGAAGCGGATTGCAGCAGGGGAGGAAACGAATTCCTATGCTGAGTTCTTGTTATCTGAAGCAAAAACACTGACTGAGGATCAGATATCTATGTTGCTCTGGGAGACAATCATTGAAACCTCGGACACTACTTTGGTTGACTATCTATATCAGCACATACTGAGTGTGTGTGGATCTGCTAAGCTTAAAGAAGAGAACCTGGCGCAGCTTCCATATCTGACTGCTGTGTTTCATGAAACTCTTCGGAAGCACAGTCCAGTCCCGATCGTCCCATTGCGATACGCTCATGAAGATACCCAGTTAGGAGGGTTCTTTGTTCCTGCAGGAAGTGAGATTGCCGTGAACATTTACGGCTGCAACATGGACAAGGAACACTGGGAAAGCCCCGAGGAATGGAAGCCAGAGAGGTTTCTTGATGAGAAATATGACCCAATGGATCTGCACAAAACAATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAAGCCTTGTAGCTTGCACAACCATTGGAAGGCTGGTTCAAGAGTTCGAGTGGAAGCTCGGGGGCGGGGAAGAAGACAAGGTCGACACTCTCGGCCTCACTGCTCGCAAGCTCCAACCGCTGCACGCAATCGTAAAGCCAAGAACCAAC

Coding sequence (CDS)

ATGGCGATCGCTTCTGATACTCTCTCCTCCATCCAGCAGCTCCTCGAGAGTCTTCACGCGCCGCCGTACGTGGCGGCGGCTGTTCTGGGTGGGGTGTCTCTGTTGCTTTCGGTGTTTGTGATCGGAGGTTGGAGGAAGAGACACCGGAATCTTCTGCCTCCGGTGCCGGCGATTCCGGGGCTGCCGGTGCTGGGGAATTTGCTGCAGTTGAAGGAGAAGAAGCCTCATAAGACGTTTGCTCGGTGGGCGGCGAAGTACGGCCCGGTCTATTCCATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCGCCGAAGTCGCCAAAGAGGCAATGGTGACGAGATACTCGTCCATTTCCAGTAGAAAACTAAGCAAAGCCCTCACAATCTTGACTTCTGATAAATGCATGGTTGCCATGAGCGATTACAATGATTTTCACAAGATGGCTAAGAGATACATATTAGCAAATGTCTTGGGAGCTAATGCTCAGAAGAGACACAGACAGAGGAGAGACACCATGATAGAGAATATTTCCAGCCACTTGTTTGCTCATGCGAAGGATTCTCCATCGCAATCTGTTAATTTTCGAGAAATCTTCGAATTGGAACTCTTTCGGCTTGCACTTAAAGAAACACTGGGGAGCGATATCAAGTCGATATATGTCAGTGAGCTCGGTACTACTGCACTGAGAGAAGATCTGTTCAGGATTTTAGTGATTGATCCGATGGAGGGTGCGATCGAAGTCGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAACAAGGGTGTGGAGGATAAAATCAGGTCGATGGAGTATCGCAGGCGAGTGACGATGAAGAGCCTTATTGAGGAGTCGAAGAAGCGGATTGCAGCAGGGGAGGAAACGAATTCCTATGCTGAGTTCTTGTTATCTGAAGCAAAAACACTGACTGAGGATCAGATATCTATGTTGCTCTGGGAGACAATCATTGAAACCTCGGACACTACTTTGGTTGACTATCTATATCAGCACATACTGAGTGTGTGTGGATCTGCTAAGCTTAAAGAAGAGAACCTGGCGCAGCTTCCATATCTGACTGCTGTGTTTCATGAAACTCTTCGGAAGCACAGTCCAGTCCCGATCGTCCCATTGCGATACGCTCATGAAGATACCCAGTTAGGAGGGTTCTTTGTTCCTGCAGGAAGTGAGATTGCCGTGAACATTTACGGCTGCAACATGGACAAGGAACACTGGGAAAGCCCCGAGGAATGGAAGCCAGAGAGGTTTCTTGATGAGAAATATGACCCAATGGATCTGCACAAAACAATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTCAAGGCAAGCCTTGTAGCTTGCACAACCATTGGAAGGCTGGTTCAAGAGTTCGAGTGGAAGCTCGGGGGCGGGGAAGAAGACAAGGTCGACACTCTCGGCCTCACTGCTCGCAAGCTCCAACCGCTGCACGCAATCGTAAAGCCAAGAACCAAC

Protein sequence

MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPGLPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISSHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNSYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLVDYLYQHILSVCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGRLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN

Homology

[Close]

BLAST of MS008813 vs. NCBI nr
Match: XP_022139822.1 (ent-kaurene oxidase, chloroplastic [Momordica charantia] >XP_022139833.1 ent-kaurene oxidase, chloroplastic [Momordica charantia])

HSP 1 Score: 985.3 bits (2546), Expect = 1.9e-283
Identity = 502/519 (96.72%), Postives = 503/519 (96.92%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG
Sbjct: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
           SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180

Query: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVI 240
           HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDI+SIYVSELGTTALREDLFRILVI
Sbjct: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIESIYVSELGTTALREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
           DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300

Query: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILSV 360
           YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQHILSV
Sbjct: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDYLYQHILSV 360

Query: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI
Sbjct: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420

Query: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
           YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR
Sbjct: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480

Query: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 504
           LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN
Sbjct: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 519

[Close]

BLAST of MS008813 vs. NCBI nr
Match: ADE06669.1 (ent-kaurene oxidase [Momordica charantia])

HSP 1 Score: 974.5 bits (2518), Expect = 3.4e-280
Identity = 500/520 (96.15%), Postives = 501/520 (96.35%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MAI SDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG
Sbjct: 1   MAITSDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQ-KRHRQRRDTMIENIS 180
           SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQ KRHRQRRDTMIENIS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQVKRHRQRRDTMIENIS 180

Query: 181 SHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILV 240
           SHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDI+SIYVSELGTTALREDLFRILV
Sbjct: 181 SHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIESIYVSELGTTALREDLFRILV 240

Query: 241 IDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETN 300
           IDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETN
Sbjct: 241 IDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETN 300

Query: 301 SYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILS 360
           SYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQHILS
Sbjct: 301 SYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDYLYQHILS 360

Query: 361 VCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVN 420
           VCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVN
Sbjct: 361 VCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVN 420

Query: 421 IYGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIG 480
           IYG NMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIG
Sbjct: 421 IYGRNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIG 480

Query: 481 RLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 504
           RLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN
Sbjct: 481 RLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 520

[Close]

BLAST of MS008813 vs. NCBI nr
Match: XP_038898390.1 (ent-kaurene oxidase [Benincasa hispida])

HSP 1 Score: 864.0 bits (2231), Expect = 6.4e-247
Identity = 430/519 (82.85%), Postives = 465/519 (89.60%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MA+ +D L+S+Q L  +L APPY AAAVLGGVSL+LSVF++   RK+ RN LPPVPAIPG
Sbjct: 1   MAVVTDPLTSMQLLANTLQAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAIPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           +PVLGNLLQLKEKKPH TFARWA KYGPVYSIRTGASTVIVLNTAEVAKEAMVTRY+SIS
Sbjct: 61  VPVLGNLLQLKEKKPHMTFARWAEKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASIS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
           SRKLSKALTILT+DKCMVAMSDYNDFHKM KRYILAN+LGANAQK+HRQRRDTMIENIS 
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANMLGANAQKKHRQRRDTMIENISR 180

Query: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVI 240
            LFAH K+ PS+SVNFR+IFE ELFRLALKETLG DI+SIYV  LGTT  REDLFR+LVI
Sbjct: 181 ELFAHVKEFPSESVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
           DPMEGAIEVDWRDFFPYLRWIPNK VE+KIR M+++R++TM+SLIEE KKRIAAGEETN 
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRKMDFQRQMTMRSLIEEQKKRIAAGEETNC 300

Query: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILSV 360
           YA+FLLSEAKTLTEDQISMLLWETIIETSDTTLV                D+LYQHILS 
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDFLYQHILSA 360

Query: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+KL EENL+QLPYLTAVFHETLRKHSPVP+VPLRYAHEDTQLGGFFVPAGSEIAVNI
Sbjct: 361 CGSSKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHEDTQLGGFFVPAGSEIAVNI 420

Query: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
           Y CNMDK+HWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKA L+ACTTIGR
Sbjct: 421 YACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 504
           + QEFEWKL  GEEDKVDTLGLTARKLQPLH +VKPR N
Sbjct: 481 MAQEFEWKLREGEEDKVDTLGLTARKLQPLHVVVKPRIN 519

[Close]

BLAST of MS008813 vs. NCBI nr
Match: KAG7024228.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 856.7 bits (2212), Expect = 1.0e-244
Identity = 426/501 (85.03%), Postives = 457/501 (91.22%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MAI +D L S+Q LL++L AP Y AA++LG +S LL+ F++  WRK  RN LPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           LPVLGNLLQLKEKKPHKTFARWA  YGP+YSIRTGASTVIVLNTAEVAKEAMVTRY+S+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
           SRKLSKALTILTSDKCMVAMSDYNDFHKM K+YILANVLGANAQKRHRQRRDTMI+NISS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDNISS 180

Query: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVI 240
            LFAH KDSPS+SVNFR IFE ELFRLALKETLG DI+SIYV  LGT   REDLFR+LVI
Sbjct: 181 QLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLGTLP-REDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
           DPMEGAIEVDWRDFFPYL+WIPNK VE+KIR M++ RRVTM SLIEE KKRIAAGEETN 
Sbjct: 241 DPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFCRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLVDYLYQHILSVCGSAKLKEENLAQLPY 360
           YA+FLLSEAKTLTE QI+MLLWETIIETSDTTLVDYLYQHI SVCGS KL E++L++LPY
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVDYLYQHIHSVCGSEKLTEDDLSKLPY 360

Query: 361 LTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYGCNMDKEHWESPEEW 420
           LTAVFHETLRKHSPVPIVPLRY HED QLGGFFVPAGSEIAVNIY CNMDK+ WESPEEW
Sbjct: 361 LTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNIYACNMDKDRWESPEEW 420

Query: 421 KPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGRLVQEFEWKLGGGEEDK 480
           KPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKA L+ACT+IGRLVQEFEWKLG GEEDK
Sbjct: 421 KPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGRLVQEFEWKLGEGEEDK 480

Query: 481 VDTLGLTARKLQPLHAIVKPR 502
           VDTLGLTARKLQPLHAIVKPR
Sbjct: 481 VDTLGLTARKLQPLHAIVKPR 500

[Close]

BLAST of MS008813 vs. NCBI nr
Match: XP_022976063.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 850.5 bits (2196), Expect = 7.3e-243
Identity = 426/517 (82.40%), Postives = 457/517 (88.39%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MAI +D L S+Q LL++L APPY AAA+LG +S LL+ F++  WRK  RN LPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           LPVLGNLLQLKEKKPHKTFARWA  YGP+YSIRTGASTVIVLNTAEVAKEAMVTRY+S+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
            RKLSKALTILTSDKCMVAMSDYNDFHKM K+YILANVLGANAQKRHRQRRD+MI+NISS
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVI 240
            LFAH KDSPS+SVNFR+IFE ELF LALKETLG DI+SIYV  LGTT  REDLFR+LVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
           D MEGAIEVDWRDFFPYL+WIPNK VE+KIR M+YRRRVTM SLIEE KKRIAAGEETN 
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILSV 360
           YA+FLLSEAKTLTE QI+MLLWETIIETSDTTLV                DYLYQHI SV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360

Query: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KL E++L++LPYLTAVFHETLRKHSPVPIVPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
           Y CNMDK+ WESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKA L+ACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPR 502
           LVQEFEWKLG GEEDKVDTLGLTARKLQPLH IVKPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPR 517

[Close]

BLAST of MS008813 vs. ExPASy Swiss-Prot
Match: A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 1.9e-177
Identity = 312/509 (61.30%), Postives = 383/509 (75.25%), Query Frame = 0


Query: 15  LESLHAPPYVAA-----AVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPGLPVLGNLLQ 74
           L SL A P  AA       +GG++L      +   RK+  +  PP P +PGLPV+GNLLQ
Sbjct: 4   LLSLQAVPAAAAIGGPVVAIGGITLFFIREYVKDQRKKSSS-FPPPPKVPGLPVIGNLLQ 63

Query: 75  LKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALT 134
           LKEKKPHKTF +W+ KYGP+YSIRTGA+T+IVLNT +VAKEAM+T+YSSIS+RKLSKALT
Sbjct: 64  LKEKKPHKTFTKWSEKYGPIYSIRTGANTMIVLNTNDVAKEAMITKYSSISTRKLSKALT 123

Query: 135 ILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISSHLFAHAKDS 194
           ILTSDK +VAMSDYN+F+K AKR++L + LG  AQKRHR  R+ MI NI     AHAK  
Sbjct: 124 ILTSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHRNLMINNICDQFLAHAKMY 183

Query: 195 PSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVIDPMEGAIEV 254
           PS++VNFR+IF+ ELF L++K+ +G D++SIYV +L TT  R+++F+ILV+DPMEGAI+V
Sbjct: 184 PSEAVNFRKIFQSELFGLSMKQAIGEDVESIYVEDLDTTLSRQEMFKILVVDPMEGAIDV 243

Query: 255 DWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNSYAEFLLSE- 314
           DWRDFFPYL+WIPN+  E+KI+ M + R   MK+LIE+ KKRIA+G+  N Y + LLSE 
Sbjct: 244 DWRDFFPYLKWIPNQHFENKIQQMHFHREAVMKALIEQQKKRIASGKAINCYLDHLLSEA 303

Query: 315 AKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILSVCGSAKLKE 374
           A TL+E QI MLLWE IIE SDTTLV                +YL   I + CG  +L E
Sbjct: 304 ADTLSEQQILMLLWEAIIEASDTTLVTTEWAMYELSKDPKRQNYLLSEIQNACGFDQLNE 363

Query: 375 ENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYGCNMDKE 434
           E L +LPYL A+F ETLRKHSPVP+VPLRY HE+TQLGG+ +P GSEIA+NIYGCNMDK 
Sbjct: 364 EKLCRLPYLAAIFQETLRKHSPVPVVPLRYVHEETQLGGYTIPEGSEIAINIYGCNMDKN 423

Query: 435 HWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGRLVQEFEWK 494
            W+SPEEW+PERF+  K D  +LHKTMAFGGGKRVCAGAL+A  ++C  IGRLVQE EW+
Sbjct: 424 VWDSPEEWRPERFVFGKDDTTELHKTMAFGGGKRVCAGALQAMTISCIAIGRLVQELEWR 483

Query: 495 LGGGEEDKVDTLGLTARKLQPLHAIVKPR 502
           LG GEE  VDTLGLT  KL PL  I+KPR
Sbjct: 484 LGDGEEANVDTLGLTTHKLHPLQTIIKPR 511

[Close]

BLAST of MS008813 vs. ExPASy Swiss-Prot
Match: Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)

HSP 1 Score: 600.5 bits (1547), Expect = 1.7e-170
Identity = 295/467 (63.17%), Postives = 363/467 (77.73%), Query Frame = 0


Query: 52  LPPVPAIPGLPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEA 111
           LP VP +PG PV+GNLLQLKEKKPHKTF RW+  YGP+YSI+ G+S++IVLN+ E AKEA
Sbjct: 41  LPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEA 100

Query: 112 MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRR 171
           MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHK+ KR +L  +LGANAQKR R  R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160

Query: 172 DTMIENISSHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALR 231
           D +IEN+SS L AHA+D P + VNFR IFE ELF +ALK+  G D++SIYV ELG T  +
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSK 220

Query: 232 EDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKR 291
           +++F++LV D MEGAI+VDWRDFFPYL+WIPNK  E +I+    RR   M +LI++  K+
Sbjct: 221 DEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQ 280

Query: 292 IAAGEETNSYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------D 351
             +  + + Y  FL+SEAKTLT++QI++L+WETIIET+DTTLV                D
Sbjct: 281 NGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQD 340

Query: 352 YLYQHILSVCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVP 411
            L + I +VCG  K KEE L+Q+PYL  VFHETLRK+SP P+VP+RYAHEDTQ+GG+ VP
Sbjct: 341 RLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVP 400

Query: 412 AGSEIAVNIYGCNMDKEHWESPEEWKPERFLDE-KYDPMDLHKTMAFGGGKRVCAGALKA 471
           AGSEIA+NIYGCNMDK+ WE PE+W PERFLD+ KY+  DLHKTMAFG GKRVCAGAL+A
Sbjct: 401 AGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQA 460

Query: 472 SLVACTTIGRLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPR 502
           SL+A   IGRLVQEFEWKL  GEE+ VDT GLT++KL PL AI+ PR
Sbjct: 461 SLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507

[Close]

BLAST of MS008813 vs. ExPASy Swiss-Prot
Match: Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 1.9e-148
Identity = 262/487 (53.80%), Postives = 347/487 (71.25%), Query Frame = 0


Query: 25  AAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPGLPVLGNLLQLKEKKPHKTFARWAA 84
           AAAV G V+        G    R R   P  PA+PGLP++GNL QLKEKKPH+TFA+WA 
Sbjct: 13  AAAVGGFVAAAALAERAGVIAPRKRPNAP--PAVPGLPIIGNLHQLKEKKPHQTFAKWAE 72

Query: 85  KYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALTILTSDKCMVAMSDYN 144
            YGP+Y+IRTGAS+V+VLN+ EVAKEAMV ++SSIS+RKLSKALT+LT DK MVA SDY 
Sbjct: 73  IYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLTRDKSMVATSDYC 132

Query: 145 DFHKMAKRYILANVLGANAQKRHRQRRDTMIENISSHLFAHAKDSPSQSVNFREIFELEL 204
           DFHKM KRY+++++LG +AQK+ R  RD MI N+ S      KD P   + FR++F+ EL
Sbjct: 133 DFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIHNMLSTFHKLVKDDPHAPLIFRDVFKDEL 192

Query: 205 FRLALKETLGSDIKSIYVSELGTTALREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNK 264
           FRL++ ++LG D+ S+YV E G    +E+++   V D M  AIEVDWRDFFPYL W+PNK
Sbjct: 193 FRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPNK 252

Query: 265 GVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNSYAEFLLSEAKTLTEDQISMLLWET 324
             E ++ + E RR   M++LI++ K+RI  GE    Y +FLL+E  TLT++Q+ ML+WE 
Sbjct: 253 SFETRVFTTETRRTAVMRALIKQQKERIVRGEAKTCYLDFLLAE-NTLTDEQLMMLVWEA 312

Query: 325 IIETSDTTLV----------------DYLYQHILSVCGSAKLKEENLAQLPYLTAVFHET 384
           +IE +DTTLV                + LYQ I  VCG   + EE+L +LPYL AVFHET
Sbjct: 313 LIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDETVTEEHLPRLPYLNAVFHET 372

Query: 385 LRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYGCNMDKEHWESPEEWKPERFLDE 444
           LR+HSPVP++P R+ HEDT+L G+ VPAG+E+ +N+YGCNM+++ WESPEEW PERF   
Sbjct: 373 LRRHSPVPLIPPRFVHEDTKLAGYDVPAGTEMVINLYGCNMNRKEWESPEEWVPERFAGG 432

Query: 445 KYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGRLVQEFEWKLGGGEEDKVDTLGLTA 496
           + +  D++KTMAFG G+R CAG+L+A+ +AC  + R VQEF W+L  G+E+KVDT+ LTA
Sbjct: 433 RLEVADMYKTMAFGAGRRACAGSLQATHIACAAVARFVQEFGWRLREGDEEKVDTVQLTA 492

[Close]

BLAST of MS008813 vs. ExPASy Swiss-Prot
Match: Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 4.2e-148
Identity = 263/494 (53.24%), Postives = 348/494 (70.45%), Query Frame = 0


Query: 24  VAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPGLPVLGNLLQLKEKKPHKTFARWA 83
           VAAA +GG     ++ V     K  RN   P PA+PGLP++GNL QLKEKKPH+TF +WA
Sbjct: 14  VAAAAVGGFIAAATLAVAP--PKNRRN---PPPAVPGLPIIGNLHQLKEKKPHQTFTKWA 73

Query: 84  AKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALTILTSDKCMVAMSDY 143
             YGP+Y+IRTGAS+V+VLN+ EVAKEAMV ++SSIS+RKLSKALT+L+ DK MVA SD 
Sbjct: 74  EIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKFSSISTRKLSKALTVLSHDKSMVATSDS 133

Query: 144 NDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISSHLFAHAKDSPSQSVNFREIFELE 203
            DFHKM KRYI+ ++LG +AQK+ R  RD +I N+ S      KD P   + FR++F+ E
Sbjct: 134 GDFHKMGKRYIMLSMLGTSAQKQFRDTRDMIINNMLSTFHQLVKDDPHAPLIFRDVFKDE 193

Query: 204 LFRLALKETLGSDIKSIYVSELGTTALREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPN 263
           LFRL++ ++LG D+ S+YV E G    +E+++   V D M  AIEVDWRDFFPYL W+PN
Sbjct: 194 LFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNATVTDMMMCAIEVDWRDFFPYLSWVPN 253

Query: 264 KGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNSYAEFLLSEAKTLTEDQISMLLWE 323
           K  E ++ + E RR   M++LI++ K+RI  GE    Y +FLL+E  TLT++Q+ ML+WE
Sbjct: 254 KSFETRVFTTESRRTAVMRALIKQQKERIVRGEARTCYLDFLLAE-NTLTDEQLMMLVWE 313

Query: 324 TIIETSDTTLV----------------DYLYQHILSVCGSAKLKEENLAQLPYLTAVFHE 383
            +IE +DTTLV                + LYQ I  VCG   + EE+L  LPYL AVF E
Sbjct: 314 ALIEAADTTLVTTEWAMYELAKNPDKQERLYQEIREVCGDEAVTEEHLPWLPYLNAVFQE 373

Query: 384 TLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYGCNMDKEHWESPEEWKPERFLD 443
           TLR+HSPVP++P R+ +EDT L G+ VPAG+E+ +N+YGCNM+K+ WESPEEW PERF  
Sbjct: 374 TLRRHSPVPLIPPRFVNEDTMLAGYDVPAGTEMVINLYGCNMNKKEWESPEEWAPERFAG 433

Query: 444 EKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGRLVQEFEWKLGGGEEDKVDTLGLT 502
            ++   D++KTMAFG G+RVCAG+L+A+ +AC  I R VQEF W+L  G+E+KVDT+ LT
Sbjct: 434 GRFKVADMYKTMAFGAGRRVCAGSLQATHIACAAIARFVQEFGWRLREGDEEKVDTVQLT 493

[Close]

BLAST of MS008813 vs. ExPASy Swiss-Prot
Match: Q0DBF4 (Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A8 PE=1 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 2.6e-134
Identity = 235/471 (49.89%), Postives = 327/471 (69.43%), Query Frame = 0


Query: 49  RNLLPPVPAIPGLPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVA 108
           +N   P PA+PGLP++GNL QLKEKKPH+TFA+W+  YGP+Y+I+TGAS V+VLN+ EVA
Sbjct: 38  KNRANPPPAVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVA 97

Query: 109 KEAMVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHR 168
           KEAM+ ++SSIS+RKL KA+++LT  K MVA+SDY D+ KMAKR I+  +LG NAQK+ R
Sbjct: 98  KEAMIDKFSSISTRKLPKAMSVLTR-KSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFR 157

Query: 169 QRRDTMIENISSHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTT 228
             R+ MI N+ S L       P   +NFR+++  ELF L+L ++LG D+ S+YV E G  
Sbjct: 158 GTRERMISNVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGRE 217

Query: 229 ALREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEES 288
             ++++F +LV + M  A+E DWRD+FPYL W+PNK  +  + + E+RR   M +LI++ 
Sbjct: 218 ISKDEIFDVLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQ 277

Query: 289 KKRIAAGEETNSYAEFLLSEAKT--LTEDQISMLLWETIIETSDTTLV------------ 348
           K+RIA GE   SY +FLL   ++  LT+DQ+ +LL E+I+  +DT LV            
Sbjct: 278 KERIARGEARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIAKN 337

Query: 349 ----DYLYQHILSVCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLG 408
               + LYQ I   CG   + E++L +LPYL AVFHETLR HSPVP++P R+ H+DT L 
Sbjct: 338 PDKQELLYQEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDDTTLA 397

Query: 409 GFFVPAGSEIAVNIYGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAG 468
           G+ + AG+++ +N+Y C+MD++ WESP EW PERFL E ++  D +KTMAFG G+R CAG
Sbjct: 398 GYDIAAGTQMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAGRRTCAG 457

Query: 469 ALKASLVACTTIGRLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPR 502
           +L+A  +AC  + RLVQE EW+L  G+ DK DT+  TA KL PLH  +KPR
Sbjct: 458 SLQAMNIACVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPR 507

[Close]

BLAST of MS008813 vs. ExPASy TrEMBL
Match: A0A6J1CGM6 (ent-kaurene oxidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111010641 PE=3 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 9.2e-284
Identity = 502/519 (96.72%), Postives = 503/519 (96.92%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG
Sbjct: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
           SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180

Query: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVI 240
           HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDI+SIYVSELGTTALREDLFRILVI
Sbjct: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIESIYVSELGTTALREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
           DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300

Query: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILSV 360
           YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQHILSV
Sbjct: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDYLYQHILSV 360

Query: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI
Sbjct: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420

Query: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
           YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR
Sbjct: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480

Query: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 504
           LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN
Sbjct: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 519

[Close]

BLAST of MS008813 vs. ExPASy TrEMBL
Match: D5LXQ4 (Ent-kaurene oxidase OS=Momordica charantia OX=3673 GN=KO PE=2 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.6e-280
Identity = 500/520 (96.15%), Postives = 501/520 (96.35%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MAI SDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG
Sbjct: 1   MAITSDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQ-KRHRQRRDTMIENIS 180
           SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQ KRHRQRRDTMIENIS
Sbjct: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQVKRHRQRRDTMIENIS 180

Query: 181 SHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILV 240
           SHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDI+SIYVSELGTTALREDLFRILV
Sbjct: 181 SHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIESIYVSELGTTALREDLFRILV 240

Query: 241 IDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETN 300
           IDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETN
Sbjct: 241 IDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETN 300

Query: 301 SYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILS 360
           SYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQHILS
Sbjct: 301 SYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELAKDPKRQDYLYQHILS 360

Query: 361 VCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVN 420
           VCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVN
Sbjct: 361 VCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVN 420

Query: 421 IYGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIG 480
           IYG NMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIG
Sbjct: 421 IYGRNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIG 480

Query: 481 RLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 504
           RLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN
Sbjct: 481 RLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 520

[Close]

BLAST of MS008813 vs. ExPASy TrEMBL
Match: A0A6J1IL06 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476578 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.5e-243
Identity = 426/517 (82.40%), Postives = 457/517 (88.39%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MAI +D L S+Q LL++L APPY AAA+LG +S LL+ F++  WRK  RN LPPVPAIPG
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVPAIPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           LPVLGNLLQLKEKKPHKTFARWA  YGP+YSIRTGASTVIVLNTAEVAKEAMVTRY+S+S
Sbjct: 61  LPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVLNTAEVAKEAMVTRYASMS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
            RKLSKALTILTSDKCMVAMSDYNDFHKM K+YILANVLGANAQKRHRQRRD+MI+NISS
Sbjct: 121 GRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDNISS 180

Query: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVI 240
            LFAH KDSPS+SVNFR+IFE ELF LALKETLG DI+SIYV  LGTT  REDLFR+LVI
Sbjct: 181 QLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRVLVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
           D MEGAIEVDWRDFFPYL+WIPNK VE+KIR M+YRRRVTM SLIEE KKRIAAGEETN 
Sbjct: 241 DLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTMMSLIEEQKKRIAAGEETNC 300

Query: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILSV 360
           YA+FLLSEAKTLTE QI+MLLWETIIETSDTTLV                DYLYQHI SV
Sbjct: 301 YADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMYELAKNPERQDYLYQHIQSV 360

Query: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS KL E++L++LPYLTAVFHETLRKHSPVPIVPLRY HED QLGGFFVPAGSEIAVNI
Sbjct: 361 CGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIAVNI 420

Query: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
           Y CNMDK+ WESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKA L+ACT+IGR
Sbjct: 421 YACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTSIGR 480

Query: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPR 502
           LVQEFEWKLG GEEDKVDTLGLTARKLQPLH IVKPR
Sbjct: 481 LVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPR 517

[Close]

BLAST of MS008813 vs. ExPASy TrEMBL
Match: A0A5D3D9I5 (Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003760 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 6.1e-243
Identity = 422/519 (81.31%), Postives = 460/519 (88.63%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MA+ +D L+S+Q L  +L APPY AAAVLGGVSL+LSVF++   RK+ RN LPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           +P+LGNLLQLKEKKPHKTFARWA  YGPVYSIRTGASTVIVLNT EVAKEAMVTRY SIS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
           SRKLSKALTILT+DKCMVAMSDYN+FHKM KRYILANVLGANAQK+HRQRRD MIENIS 
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVI 240
            LFAH K+ PS +VNFR+IFE ELFRLALKETLG DI+SIYV  LGTT  REDLFRILVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
           DPMEGAIEVDWRDFFPYLRWIPNK VE+KIR+M++RRR+TMKSLI+E K+RIAAGEETN 
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILSV 360
           YA+FLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQ I SV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+ L EENL+QLPYLTA+FHETLRKHSPVP+VPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
           Y CNMDK HWE+PEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKA L+ACTTIGR
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 504
           +VQEFEWKL  GEE+KVDTLGLTARKLQPLH ++KPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]

BLAST of MS008813 vs. ExPASy TrEMBL
Match: A0A1S3BTZ0 (ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 6.1e-243
Identity = 422/519 (81.31%), Postives = 460/519 (88.63%), Query Frame = 0


Query: 1   MAIASDTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSVFVIGGWRKRHRNLLPPVPAIPG 60
           MA+ +D L+S+Q L  +L APPY AAAVLGGVSL+LSVF++   RK+ RN LPPVPA+PG
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVPAVPG 60

Query: 61  LPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSIS 120
           +P+LGNLLQLKEKKPHKTFARWA  YGPVYSIRTGASTVIVLNT EVAKEAMVTRY SIS
Sbjct: 61  VPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIVLNTTEVAKEAMVTRYGSIS 120

Query: 121 SRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRRDTMIENISS 180
           SRKLSKALTILT+DKCMVAMSDYN+FHKM KRYILANVLGANAQK+HRQRRD MIENIS 
Sbjct: 121 SRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGANAQKKHRQRRDAMIENISR 180

Query: 181 HLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVI 240
            LFAH K+ PS +VNFR+IFE ELFRLALKETLG DI+SIYV  LGTT  REDLFRILVI
Sbjct: 181 ELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRILVI 240

Query: 241 DPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNS 300
           DPMEGAIEVDWRDFFPYLRWIPNK VE+KIR+M++RRR+TMKSLI+E K+RIAAGEETN 
Sbjct: 241 DPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTMKSLIQEPKRRIAAGEETNC 300

Query: 301 YAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHILSV 360
           YA+FLLSEAKTLTEDQISMLLWETIIETSDTTLV                DYLYQ I SV
Sbjct: 301 YADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMYELSKDPKRQDYLYQQIQSV 360

Query: 361 CGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNI 420
           CGS+ L EENL+QLPYLTA+FHETLRKHSPVP+VPLRYAHEDTQLGG+FVPAGSEIAVNI
Sbjct: 361 CGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNI 420

Query: 421 YGCNMDKEHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKASLVACTTIGR 480
           Y CNMDK HWE+PEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKA L+ACTTIGR
Sbjct: 421 YACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGR 480

Query: 481 LVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPRTN 504
           +VQEFEWKL  GEE+KVDTLGLTARKLQPLH ++KPR N
Sbjct: 481 MVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

[Close]

BLAST of MS008813 vs. TAIR 10
Match: AT5G25900.1 (GA requiring 3 )

HSP 1 Score: 600.5 bits (1547), Expect = 1.2e-171
Identity = 295/467 (63.17%), Postives = 363/467 (77.73%), Query Frame = 0


Query: 52  LPPVPAIPGLPVLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEA 111
           LP VP +PG PV+GNLLQLKEKKPHKTF RW+  YGP+YSI+ G+S++IVLN+ E AKEA
Sbjct: 41  LPSVPVVPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEA 100

Query: 112 MVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANVLGANAQKRHRQRR 171
           MVTR+SSIS+RKLS ALT+LT DK MVA SDY+DFHK+ KR +L  +LGANAQKR R  R
Sbjct: 101 MVTRFSSISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYR 160

Query: 172 DTMIENISSHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALR 231
           D +IEN+SS L AHA+D P + VNFR IFE ELF +ALK+  G D++SIYV ELG T  +
Sbjct: 161 DALIENVSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSK 220

Query: 232 EDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKR 291
           +++F++LV D MEGAI+VDWRDFFPYL+WIPNK  E +I+    RR   M +LI++  K+
Sbjct: 221 DEIFKVLVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQ 280

Query: 292 IAAGEETNSYAEFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------D 351
             +  + + Y  FL+SEAKTLT++QI++L+WETIIET+DTTLV                D
Sbjct: 281 NGSESDDDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQD 340

Query: 352 YLYQHILSVCGSAKLKEENLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVP 411
            L + I +VCG  K KEE L+Q+PYL  VFHETLRK+SP P+VP+RYAHEDTQ+GG+ VP
Sbjct: 341 RLCKEIQNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVP 400

Query: 412 AGSEIAVNIYGCNMDKEHWESPEEWKPERFLDE-KYDPMDLHKTMAFGGGKRVCAGALKA 471
           AGSEIA+NIYGCNMDK+ WE PE+W PERFLD+ KY+  DLHKTMAFG GKRVCAGAL+A
Sbjct: 401 AGSEIAINIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQA 460

Query: 472 SLVACTTIGRLVQEFEWKLGGGEEDKVDTLGLTARKLQPLHAIVKPR 502
           SL+A   IGRLVQEFEWKL  GEE+ VDT GLT++KL PL AI+ PR
Sbjct: 461 SLMAGIAIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507

[Close]

BLAST of MS008813 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 159.1 bits (401), Expect = 9.5e-39
Identity = 136/508 (26.77%), Postives = 238/508 (46.85%), Query Frame = 0


Query: 35  LLSVFVI-----GGWRKRHRNLLPPVPAIPGLPVLGNLLQLKEKKPHKTFARWAAKYGPV 94
           +LS F+I      G   R    LPP P  P LP++GN + L  K PH++FA  +  YGPV
Sbjct: 16  ILSCFLIFTTTRSGRISRGATALPPGP--PRLPIIGN-IHLVGKHPHRSFAELSKTYGPV 75

Query: 95  YSIRTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKAL-TILTSDKCMVAMSDYNDFHK 154
            S++ G+   +V+ + E A+E + T    +S+R  + A+ +I   D  +V +   +   +
Sbjct: 76  MSLKLGSLNTVVIASPEAAREVLRTHDQILSARSPTNAVRSINHQDASLVWLPSSSARWR 135

Query: 155 MAKRYILANVLGANAQKRHRQRRDTMIENISSHLFAHAKDSPSQSVNFREIFELELFRLA 214
           + +R  +  +L     +  +  R   ++ + S +        S+S +  E   +++ R+A
Sbjct: 136 LLRRLSVTQLLSPQRIEATKALRMNKVKELVSFI--------SESSDREE--SVDISRVA 195

Query: 215 LKETLGSDIKSIYVSELGTTALREDLFRI--LVIDPMEGAIEVDWRDFFPYLRWIPNKGV 274
              TL      ++  +LG+   +  +  +   VI  M+ A   D  ++FP+LR++  +G 
Sbjct: 196 FITTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGN 255

Query: 275 EDKIRSMEYR-----RRVTMKSLIEESKKRIAAGEETNSYAEFLL---SEAKTLTEDQIS 334
               +    R     R      + E+S +        N + + LL    +   L+   I 
Sbjct: 256 VKTFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIE 315

Query: 335 MLLWETI---IETSDTTLVDYLYQHILSVCGSAK--------------LKEENLAQLPYL 394
            LL +      +TS +TL   + + + +    AK              ++E ++++LPYL
Sbjct: 316 HLLLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYL 375

Query: 395 TAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYGCNMDKEHWESPEEWK 454
            AV  ET R H+PVP++  R A  D ++ GF V   +++ VN++    D   W++P +++
Sbjct: 376 QAVVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFE 435

Query: 455 PERFLDEKYDPMDL-HKTMAFGGGKRVCAGALKASLVACTTIGRLVQEFEWKLGGG---- 504
           PERFL +  D     ++   FG G+R+C G   A       +  L+  F+WKL  G    
Sbjct: 436 PERFLGKDMDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSE 495

[Close]

BLAST of MS008813 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 151.8 bits (382), Expect = 1.5e-36
Identity = 134/507 (26.43%), Postives = 226/507 (44.58%), Query Frame = 0


Query: 35  LLSVFVIGGWRKR---HRNLLPPVPAIPGLPVLGNLLQLKEKKPHKTFARWAAKYGPVYS 94
           +LS F+I     R    R ++P  P  P LP++GN + L  + PH +FA  +  YGP+ S
Sbjct: 16  VLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGN-IHLVGRNPHHSFADLSKTYGPIMS 75

Query: 95  IRTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDFHKMAK 154
           ++ G+   +V+ + E A+E + T    +SSR  + ++  +  DK  V           + 
Sbjct: 76  LKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVVW-----LPPSSS 135

Query: 155 RYILANVLGANA--QKRHRQRRDTMIENISSHLFAHAKDSPSQSVNFREIFELELFRLAL 214
           R+ L   L A      +  +   T+ EN    L +   +S  +         +++ R   
Sbjct: 136 RWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREE------AVDISRATF 195

Query: 215 KETLGSDIKSIYVSELGT-TALREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKGVED 274
              L      ++  +LG   + +  +F+  VI  ME     D  +FFP+L ++  +G   
Sbjct: 196 ITALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRK 255

Query: 275 KIRSMEYRRRVTMKSLIEESKKRIAAGEETNS-------YAEFLL-----SEAKTLTEDQ 334
            +++   R     +  I ++K    +  +TNS       + + LL      EA+  T D 
Sbjct: 256 TLKACSERLFKVFRGFI-DAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDI 315

Query: 335 ISMLL------WETIIETSDTTLVDYLYQHILSVCGSAKL----------KEENLAQLPY 394
           + +LL       +T   T +  + + L      V   A++          +E +++ LPY
Sbjct: 316 VHLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPY 375

Query: 395 LTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYGCNMDKEHWESPEEW 454
           L AV  ET R H   P++  R A  D ++ GF VP  +++ VN++    D   WE+   +
Sbjct: 376 LQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRF 435

Query: 455 KPERFLDEKYDPMDL-HKTMAFGGGKRVCAGALKASLVACTTIGRLVQEFEWKL----GG 502
           KPERFL +  D     ++   FG G+R+C G   A       +  L+  F+WKL    G 
Sbjct: 436 KPERFLGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGS 495

[Close]

BLAST of MS008813 vs. TAIR 10
Match: AT4G22690.1 (cytochrome P450, family 706, subfamily A, polypeptide 1 )

HSP 1 Score: 147.1 bits (370), Expect = 3.7e-35
Identity = 135/529 (25.52%), Postives = 237/529 (44.80%), Query Frame = 0


Query: 6   DTLSSIQQLLESLHAPPYVAAAVLGGVSLLLSV--FVIGGWRKRHRNLLPPVPAIP-GLP 65
           +T SS   L + L+     ++ +LG  +LL  V  F I G  K      PP+P  P  LP
Sbjct: 35  ETASSNFSLSQILNIEEPYSSVMLGVAALLAVVCYFWIQGKSKSKNG--PPLPPGPWPLP 94

Query: 66  VLGNLLQLKEKKPHKTFARWAAKYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSISSR 125
           ++GNL  L     H  F     K+GP+  I  G+   IV+++ ++A+E + T   + ++ 
Sbjct: 95  IVGNLPFLNSDVLHTQFQALTLKHGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANH 154

Query: 126 KLSKALTILTSDKCMVAMSDYNDFHKMAKRYILANV-----LGANAQKRHRQRRDTMIEN 185
            L +   I T     +  S Y    +  ++  +  +     L A+   R  + R T++  
Sbjct: 155 DLPEVGKINTYGGEDILWSPYGTHWRRLRKLCVMKMFTTPTLEASYSTRREETRQTIV-- 214

Query: 186 ISSHLFAHAKDSPSQSVNFREIFELELFRLALKETLGSDIKSIYVSELGTTALREDLFRI 245
              H+   A++     VN  E   L +F +  +   G+ ++    + LG         + 
Sbjct: 215 ---HMSEMARE--GSPVNLGEQIFLSIFNVVTRMMWGATVEGDERTSLGNE------LKT 274

Query: 246 LVIDPMEGAIEVDWRDFFPYLRWIPNKGVEDKIRSMEYRRRVTMKSLIEESKKRIA-AGE 305
           L+ D  +     ++ DFFP       +G+  +++    +  +    ++E   K +    E
Sbjct: 275 LISDISDIEGIQNYSDFFPLFSRFDFQGLVKQMKGHVKKLDLLFDRVMESHVKMVGKKSE 334

Query: 306 ETNSYAEFLL-----SEAKTLTEDQISMLLWETIIETSDTTL--VDYLYQHILS------ 365
           E   + ++LL      E   L+   +  LL + ++   DT++   ++    I+S      
Sbjct: 335 EEEDFLQYLLRVKDDDEKAPLSMTHVKSLLMDMVLGGVDTSVNASEFAMAEIVSRPEVLN 394

Query: 366 --------VCGSAKLKEE-NLAQLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFV 425
                   V G   + EE +L +LPYL AV  ETLR H  +P++      E + + G+ V
Sbjct: 395 KIRLELDQVVGKDNIVEESHLPKLPYLQAVMKETLRLHPTLPLLVPHRNSETSVVAGYTV 454

Query: 426 PAGSEIAVNIYGCNMDKEHWESPEEWKPERFLDEKYD-PMDLHKTMAFGGGKRVCAGALK 485
           P  S+I +N++  + D ++W+ P E+KPERFL+   D      K + FG G+R+CA    
Sbjct: 455 PKDSKIFINVWAIHRDPKNWDEPNEFKPERFLENSLDFNGGDFKYLPFGSGRRICAAINM 514

Query: 486 ASLVACTTIGRLVQEFEWKLGGGEEDKV-DTLGLTARKLQPLHAIVKPR 502
           A  +    I  L+  F+WK   G++ +V +  GL  +   PL AI  PR
Sbjct: 515 AERLVLFNIASLLHSFDWKAPQGQKFEVEEKFGLVLKLKSPLVAIPVPR 548

[Close]

BLAST of MS008813 vs. TAIR 10
Match: AT4G13770.1 (cytochrome P450, family 83, subfamily A, polypeptide 1 )

HSP 1 Score: 146.7 bits (369), Expect = 4.9e-35
Identity = 126/507 (24.85%), Postives = 241/507 (47.53%), Query Frame = 0


Query: 28  VLGGVSL-LLSVFVIGGWRKRHRNLLPPVPAIPGLPVLGNLLQLKEKKPHKTFARWAAKY 87
           ++G V+L  + +F +    K  R  LPP P+   LPV+GNLLQL++  P + FA WA KY
Sbjct: 5   IIGVVALAAVLLFFLYQKPKTKRYKLPPGPS--PLPVIGNLLQLQKLNPQRFFAGWAKKY 64

Query: 88  GPVYSIRTGASTVIVLNTAEVAKEAMVTRYSSISSRKLSKALTILTSDKCMVAMSDYNDF 147
           GP+ S R G+ T++V+++AE+AKE + T+  + + R   +    ++  +  +A++ Y  +
Sbjct: 65  GPILSYRIGSRTMVVISSAELAKELLKTQDVNFADRPPHRGHEFISYGRRDMALNHYTPY 124

Query: 148 H----KMAKRYILANVLGANAQKRHRQRRDTMIENISSHLFAHAKDSPSQSVNFREIFEL 207
           +    KM   ++ +    A  +    +    M++ I+      A D  S+ V+  E+   
Sbjct: 125 YREIRKMGMNHLFSPTRVATFKHVREEEARRMMDKINK-----AADK-SEVVDISELMLT 184

Query: 208 ELFRLALKETLGSDIKSIYVSELGTTALREDLFRILVIDPMEGAIEVDWRDFFPYLRWIP 267
               +  ++  G        +E G    R   F  ++        ++ + DFFPY  ++ 
Sbjct: 185 FTNSVVCRQAFGKK-----YNEDGEEMKR---FIKILYGTQSVLGKIFFSDFFPYCGFLD 244

Query: 268 N-KGVEDKIRSMEYRRRVTMKSLIEESKKRIAAGEETNSYAEFLLS------EAKTLTED 327
           +  G+   ++    R+   ++ ++ E+        ET S  + L+        A   T D
Sbjct: 245 DLSGLTAYMKECFERQDTYIQEVVNETLDPKRVKPETESMIDLLMGIYKEQPFASEFTVD 304

Query: 328 QISMLLWETIIETSDTTL------VDYL--YQHILSVC-----------GSAKLKEENLA 387
            +  ++ + ++  +DT        + YL  Y  +L              GS  + E+++ 
Sbjct: 305 NVKAVILDIVVAGTDTAAAAVVWGMTYLMKYPQVLKKAQAEVREYMKEKGSTFVTEDDVK 364

Query: 388 QLPYLTAVFHETLRKHSPVPIVPLRYAHEDTQLGGFFVPAGSEIAVNIYGCNMDKEHW-E 447
            LPY  A+  ETLR    +P++  R   +DT++ G+ +PAG+ + VN +  + D++ W  
Sbjct: 365 NLPYFRALVKETLRIEPVIPLLIPRACIQDTKIAGYDIPAGTTVNVNAWAVSRDEKEWGP 424

Query: 448 SPEEWKPERFLDEKYDPMDL-HKTMAFGGGKRVCAGALKASLVACTTIGRLVQEFEWKLG 498
           +P+E++PERFL+++ D     ++ + FG G+R+C G    + +       L+  F +KL 
Sbjct: 425 NPDEFRPERFLEKEVDFKGTDYEFIPFGSGRRMCPGMRLGAAMLEVPYANLLLSFNFKLP 484

The following BLAST results are available for this feature:

BLAST of MS008813 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139822.1	1.9e-283	96.72	ent-kaurene oxidase, chloroplastic [Momordica charantia] >XP_022139833.1 ent-kau...	[more]
ADE06669.1	3.4e-280	96.15	ent-kaurene oxidase [Momordica charantia]	[more]
XP_038898390.1	6.4e-247	82.85	ent-kaurene oxidase [Benincasa hispida]	[more]
KAG7024228.1	1.0e-244	85.03	Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022976063.1	7.3e-243	82.40	ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of MS008813 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0G2RKY1	1.9e-177	61.30	Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1	[more]
Q93ZB2	1.7e-170	63.17	Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV...	[more]
Q5Z5R4	1.9e-148	53.80	Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 ...	[more]
Q5Z5R7	4.2e-148	53.24	Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19...	[more]
Q0DBF4	2.6e-134	49.89	Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of MS008813 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CGM6	9.2e-284	96.72	ent-kaurene oxidase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101064...	[more]
D5LXQ4	1.6e-280	96.15	Ent-kaurene oxidase OS=Momordica charantia OX=3673 GN=KO PE=2 SV=1	[more]
A0A6J1IL06	3.5e-243	82.40	ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476...	[more]
A0A5D3D9I5	6.1e-243	81.31	Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G...	[more]
A0A1S3BTZ0	6.1e-243	81.31	ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 ...	[more]

BLAST of MS008813 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25900.1	1.2e-171	63.17	GA requiring 3	[more]
AT2G45560.1	9.5e-39	26.77	cytochrome P450, family 76, subfamily C, polypeptide 1	[more]
AT2G45570.1	1.5e-36	26.43	cytochrome P450, family 76, subfamily C, polypeptide 2	[more]
AT4G22690.1	3.7e-35	25.52	cytochrome P450, family 706, subfamily A, polypeptide 1	[more]
AT4G13770.1	4.9e-35	24.85	cytochrome P450, family 83, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 83..102 score: 35.31 coord: 448..471 score: 25.15 coord: 362..380 score: 32.32 coord: 438..448 score: 51.84 coord: 403..427 score: 35.47 coord: 107..128 score: 21.39 coord: 283..300 score: 26.56
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 45..503 e-value: 4.0E-80 score: 271.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 53..501
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 59..332 e-value: 4.2E-17 score: 62.1 coord: 337..484 e-value: 7.1E-37 score: 127.2
IPR044225	Ent-kaurene oxidase, chloroplastic	PANTHER	PTHR47283	ENT-KAURENE OXIDASE, CHLOROPLASTIC	coord: 25..502
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 441..450

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008813.1	MS008813.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010241	ent-kaurene oxidation to kaurenoic acid
biological_process	GO:0009686	gibberellin biosynthetic process
molecular_function	GO:0052615	ent-kaurene oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen