MS008722 (gene) Bitter gourd (TR) v1

BLAST of MS008722 vs. NCBI nr
Match: XP_022138249.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 959.5 bits (2479), Expect = 1.1e-275
Identity = 467/512 (91.21%), Postives = 490/512 (95.70%), Query Frame = 0

Query: 3   MEETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQML 62
           MEETLQF  FLIPL LLF LIHVF FKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQML
Sbjct: 1   MEETLQFSRFLIPLALLFALIHVFNFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQML 60

Query: 63  QNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVG 122
           QNLS+KYGPI+SLS GFR VVVVSSPSAAQECFTKNDI+FA+RPRMISGKILNYNYT+VG
Sbjct: 61  QNLSKKYGPILSLSLGFRSVVVVSSPSAAQECFTKNDIVFANRPRMISGKILNYNYTSVG 120

Query: 123 ATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSRL 182
           ATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQ+EVKLWLKNLY+TTGQ NV+VEIR++L
Sbjct: 121 ATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQQEVKLWLKNLYQTTGQNNVQVEIRTKL 180

Query: 183 TELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQ 242
           TELSFNIALRMMTGKRYFGVDVEDSEEGR+LKKVMK+V LLSGASYPADFLSVLKLIDYQ
Sbjct: 181 TELSFNIALRMMTGKRYFGVDVEDSEEGRKLKKVMKEVSLLSGASYPADFLSVLKLIDYQ 240

Query: 243 GFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDH 302
           GFRKRLWKVWAGADGFAQSLI+GRRERK SCSFSPEE RKNMIDTMLSLQDSDP YYKDH
Sbjct: 241 GFRKRLWKVWAGADGFAQSLIDGRRERKASCSFSPEESRKNMIDTMLSLQDSDPGYYKDH 300

Query: 303 IIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNL 362
           IIKGQILTLLAAGTDTTAGTIEWGMSLLLNH T MKK WSEIN+YVGEQRLVEEADL NL
Sbjct: 301 IIKGQILTLLAAGTDTTAGTIEWGMSLLLNHRTIMKKVWSEINEYVGEQRLVEEADLTNL 360

Query: 363 PYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPT 422
           P+LQAIINET R+FPALPILVPRESSEDCTI GY++PKGTMLVVN WAIHRDPKVW+DPT
Sbjct: 361 PFLQAIINETNRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNVWAIHRDPKVWQDPT 420

Query: 423 SFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEID 482
           SF+PERFENG++VE NKLLPFGMGRRACPG+GLAHRVVGLALATLIQCFEWDKIGPNEID
Sbjct: 421 SFQPERFENGEVVEANKLLPFGMGRRACPGSGLAHRVVGLALATLIQCFEWDKIGPNEID 480

Query: 483 LSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
           L QGNGLTMPKA PLEAMCKPRAPMINLLSQL
Sbjct: 481 LFQGNGLTMPKAHPLEAMCKPRAPMINLLSQL 512

BLAST of MS008722 vs. NCBI nr
Match: XP_022136931.1 (isoflavone 2'-hydroxylase-like [Momordica charantia])

HSP 1 Score: 926.8 bits (2394), Expect = 8.2e-266
Identity = 453/512 (88.48%), Postives = 473/512 (92.38%), Query Frame = 0

Query: 3   MEETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQML 62
           MEE LQF  FL+PL LLF LIHVF FK KKVSSKNLPP PPSLPVIGHLHLLREPFHQML
Sbjct: 1   MEEILQFSRFLVPLALLFALIHVFNFKFKKVSSKNLPPXPPSLPVIGHLHLLREPFHQML 60

Query: 63  QNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVG 122
           QNLSQ+YGPI+SLS GFR VVVVSSP AAQECFTKNDI+FA+RPRMISGKILNYNYT VG
Sbjct: 61  QNLSQRYGPILSLSLGFRSVVVVSSPIAAQECFTKNDIVFANRPRMISGKILNYNYTTVG 120

Query: 123 ATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSRL 182
           ATAYGQHWRIMRRIATT+LLSTHRL SFINLRQEEVKLWLKNLYR TGQ NVRVEIRSRL
Sbjct: 121 ATAYGQHWRIMRRIATTELLSTHRLISFINLRQEEVKLWLKNLYRITGQNNVRVEIRSRL 180

Query: 183 TELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQ 242
           TELSFNI LRMMTGKRYFGV+V+DSEE RELKKVMK+V LLSG SYPADFLSVL+LIDYQ
Sbjct: 181 TELSFNIVLRMMTGKRYFGVEVKDSEEERELKKVMKEVSLLSGMSYPADFLSVLRLIDYQ 240

Query: 243 GFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDH 302
           GFRKRLWKVWAGADGF Q LI+GRRE K SCSFS EE  KNMIDTMLSLQDSDP YYKDH
Sbjct: 241 GFRKRLWKVWAGADGFVQXLIDGRREMKASCSFSLEENWKNMIDTMLSLQDSDPDYYKDH 300

Query: 303 IIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNL 362
           IIKGQILTLL AGTDTTAGTIEWGMSLLLNHPT MKKAWSEINDYVGEQRLVEEADL NL
Sbjct: 301 IIKGQILTLLVAGTDTTAGTIEWGMSLLLNHPTIMKKAWSEINDYVGEQRLVEEADLANL 360

Query: 363 PYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPT 422
           PYLQAIINETYR+FPALPILVPRESSEDCTI GY+VPKGTMLVVN WAIHRDP+VW+DPT
Sbjct: 361 PYLQAIINETYRLFPALPILVPRESSEDCTIGGYDVPKGTMLVVNMWAIHRDPQVWQDPT 420

Query: 423 SFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEID 482
            F+PERFENG++VEVNKLLPFGMGRRACPGAGLAHRVVGL LATLIQCFEWD+I PNEID
Sbjct: 421 RFQPERFENGEVVEVNKLLPFGMGRRACPGAGLAHRVVGLTLATLIQCFEWDRIDPNEID 480

Query: 483 LSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
           L QGNGLTMPKA PLEAMCKPR PMI+LLS L
Sbjct: 481 LXQGNGLTMPKAHPLEAMCKPRGPMISLLSHL 512

BLAST of MS008722 vs. NCBI nr
Match: XP_022940149.1 (cytochrome P450 81E8-like [Cucurbita moschata])

HSP 1 Score: 802.0 bits (2070), Expect = 3.1e-228
Identity = 398/516 (77.13%), Postives = 454/516 (87.98%), Query Frame = 0

Query: 1   ASMEETLQFFHFLIP-LTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFH 60
           ++MEETL F +F +P L L+  L+ +FKF  +K   KNLPPGPPSLPVIGHLHLL+EPFH
Sbjct: 3   STMEETLLFSNFEVPLLVLILALLLIFKF--RKAEHKNLPPGPPSLPVIGHLHLLKEPFH 62

Query: 61  QMLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYT 120
           QMLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YT
Sbjct: 63  QMLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYT 122

Query: 121 AVGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIR 180
           AVGATAYGQHWRIMRRIATT+LLST+RLN+ IN+RQ+EVKLWLK+LYR +G+ NVRVEIR
Sbjct: 123 AVGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIR 182

Query: 181 SRLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKL 240
           S+LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYPADFLSVLKL
Sbjct: 183 SKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 242

Query: 241 IDYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHY 300
           +DYQGFRKRL KV    D FAQSLI+ RR +K     + + ERK MID++LS+Q  +P Y
Sbjct: 243 VDYQGFRKRLAKVRVDGDAFAQSLIDERRNKK-----ADDNERKTMIDSLLSMQQMEPEY 302

Query: 301 YKDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEAD 360
           YKD IIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG++RLVEE D
Sbjct: 303 YKDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMD 362

Query: 361 LVNLPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVW 420
           L  L YLQ IINETYR+FPALPILVPRESSEDCTI G++VPKGTMLVVNAWAIHRDPKVW
Sbjct: 363 LNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVW 422

Query: 421 EDPTSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGP 480
           EDP SFRPERF+ G+ VEV+KLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEW+++  
Sbjct: 423 EDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN 482

Query: 481 NEIDLSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
            E+DL+ G GLTMPKA+PL AM KPRAP+I+LLSQL
Sbjct: 483 KEVDLTPGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

BLAST of MS008722 vs. NCBI nr
Match: XP_022982228.1 (isoflavone 3'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 796.6 bits (2056), Expect = 1.3e-226
Identity = 394/515 (76.50%), Postives = 445/515 (86.41%), Query Frame = 0

Query: 1   ASMEETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQ 60
           ++MEETL  F     L L+  L  +  FK +K   KNLPPGPPSLPVIGHLHLL+EPFHQ
Sbjct: 3   STMEETLLLFSNFEVLLLILVLALLMIFKFRKADHKNLPPGPPSLPVIGHLHLLKEPFHQ 62

Query: 61  MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 120
           MLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YTA
Sbjct: 63  MLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTA 122

Query: 121 VGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRS 180
           VGATAYGQHWRIMRRIATT+LLSTHRLN+ IN+RQ+EVKLWLK+LYR TG+ NVRVEIRS
Sbjct: 123 VGATAYGQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRITGKNNVRVEIRS 182

Query: 181 RLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLI 240
           +LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYPADFLSVLKL+
Sbjct: 183 KLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLV 242

Query: 241 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 300
           DYQGFRK+L KV    D FAQSLI+ RR +K     +   ERK MID++LS+Q  +P YY
Sbjct: 243 DYQGFRKKLAKVRVDGDAFAQSLIDERRNKK-----ADHNERKTMIDSLLSMQQMEPEYY 302

Query: 301 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADL 360
           KDHIIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG++RLVEE DL
Sbjct: 303 KDHIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDL 362

Query: 361 VNLPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWE 420
             L YLQ IINETYR+FPALPILVPRESSEDCTI G++VP GTMLVVNAWAIHRDPKVWE
Sbjct: 363 NQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPKVWE 422

Query: 421 DPTSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPN 480
           DP SFRPERF+ G+ VEV+KLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEW+++   
Sbjct: 423 DPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDNK 482

Query: 481 EIDLSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
           E+DL+ G GLTMPK+ PL AM KPRAP+ +LLSQL
Sbjct: 483 EVDLTPGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512

BLAST of MS008722 vs. NCBI nr
Match: KAG7037779.1 (Cytochrome P450 81E8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 796.6 bits (2056), Expect = 1.3e-226
Identity = 394/515 (76.50%), Postives = 451/515 (87.57%), Query Frame = 0

Query: 1   ASMEETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQ 60
           ++MEETL F +F + L +L   + +  FK +K  +KNLPPGPPS+PVIGHLHLL+EPFHQ
Sbjct: 17  STMEETLLFSNFEVTLLVLILGV-LLIFKFRKAENKNLPPGPPSIPVIGHLHLLKEPFHQ 76

Query: 61  MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 120
           MLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YTA
Sbjct: 77  MLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTA 136

Query: 121 VGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRS 180
           VGATAYGQHWRIMRRIATT+LLST+RLN+ IN+RQ+EVKLWLK+LYR +G+ NVRVEIRS
Sbjct: 137 VGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIRS 196

Query: 181 RLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLI 240
           +LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYPADFLSVLKL+
Sbjct: 197 KLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLV 256

Query: 241 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 300
           DYQGFRKRL KV    D FAQSLI+ RR +K     + + ERK MID++LS+Q  +P YY
Sbjct: 257 DYQGFRKRLAKVRVDGDAFAQSLIDERRNKK-----ADDNERKTMIDSLLSMQQMEPEYY 316

Query: 301 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADL 360
           KD IIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG++RLVEE DL
Sbjct: 317 KDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDL 376

Query: 361 VNLPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWE 420
             L YLQ IINETYR+FPALPILVPRESSEDCTI G++VPKGTMLVVNAWAIHRDPKVWE
Sbjct: 377 NQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVWE 436

Query: 421 DPTSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPN 480
           DP SFRPERF+ G+ VEV+KLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEW+++   
Sbjct: 437 DPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDNK 496

Query: 481 EIDLSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
           E+DL+ G GLTMPKA PL AM KPRAP+I+LLSQL
Sbjct: 497 EVDLTPGIGLTMPKASPLVAMFKPRAPIIHLLSQL 525

BLAST of MS008722 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 1.0e-149
Identity = 267/504 (52.98%), Postives = 362/504 (71.83%), Query Frame = 0

Query: 17  TLLFTLIHV----FKFKLKKVS--SKNLPPGPP-SLPVIGHLHLLREPFHQMLQNLSQKY 76
           TLL+T + V       KL  VS   +NLPP P  +LPVIGHLHL+ +  H+ L +LS+KY
Sbjct: 5   TLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 64

Query: 77  GPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVGATAYGQH 136
           G + SL  G R V+VVSSP+AA+ECFTKNDI+FA+RP  I GK + YNYT +  + YG+H
Sbjct: 65  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 124

Query: 137 WRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSRLTELSFNI 196
           WR +RR+A  ++ S   LN F+++R++EVK  L +LY+++GQ   +VE++S+L+ELSFN+
Sbjct: 125 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNV 184

Query: 197 ALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQGFRKRLW 256
            +RM+ GKRYFG DV DS+E +  + ++ +V   +GAS P DF+  L+ ID++ + K++ 
Sbjct: 185 TMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 244

Query: 257 KVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIKGQIL 316
           K+    D F Q LI+  R  K             MID +LSLQ+S P YY D IIKG I+
Sbjct: 245 KISQEMDAFLQRLIHESRINK---------NNVTMIDHLLSLQESQPEYYTDQIIKGIIM 304

Query: 317 TLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNLPYLQAII 376
            LL AGTDT+A T+EW MSLLLNHP  ++KA +EI   VG  RL+EE DL  L YL  II
Sbjct: 305 VLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNII 364

Query: 377 NETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPTSFRPERF 436
           +ET+R+ PA P+LVP ESS+DC ++GY+VPKGT+L+VNAWAIHRDP+ W++PT F+PER 
Sbjct: 365 SETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPER- 424

Query: 437 ENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEIDLSQGNGL 496
             G  +E +KL+PFGMGRR+CPG+GLA RVVGL L  LIQCFEW +IG  +ID+++G+GL
Sbjct: 425 HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGL 484

Query: 497 TMPKAQPLEAMCKPRAPMINLLSQ 514
           TMPKAQPLEA+CKPR  +  ++S+
Sbjct: 485 TMPKAQPLEALCKPRNILHKVVSE 497

BLAST of MS008722 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.4e-137
Identity = 241/499 (48.30%), Postives = 335/499 (67.13%), Query Frame = 0

Query: 9   FFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQMLQNLSQK 68
           F++ L+ L+ + T+  + K   +++  KNLPPGPP++P+IG+LH L+ P H+    LSQ 
Sbjct: 3   FYYSLLSLSFIITIKILLKITSRRL--KNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQT 62

Query: 69  YGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVGATAYGQ 128
           YG I SL FG R VVVVSSPS A ECFTKNDII A+RPR ++GK + YNYT +G+ +YG 
Sbjct: 63  YGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGD 122

Query: 129 HWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYR---TTGQINVRVEIRSRLTEL 188
           HWR +RRI T D+LS +RLNSF+ +R++E    ++ L +   + G    +VE+R RLTE+
Sbjct: 123 HWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEM 182

Query: 189 SFNIALRMMTGKRYFG--VDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQG 248
           +FN  +RM++GKRY+G   DV D EE ++ ++++ ++  L GA+   DFL +L+++D   
Sbjct: 183 TFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDN 242

Query: 249 FRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHI 308
             KR  ++   ++ F + LI   R   +        +   MID +L L +S P YY DH+
Sbjct: 243 LEKRCKRIAKRSNAFLEGLIEEHRRGNI------HSDGGTMIDHLLKLSESQPEYYSDHL 302

Query: 309 IKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNLP 368
           IKG I  +L AGTDT+A TIEW MS LLNHP  +KKA  E++  +G+ +LV+E DL  LP
Sbjct: 303 IKGLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLP 362

Query: 369 YLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPTS 428
           YLQ II+ET R+ P  P+L+P  SSEDCTI  +NVPK T+++ N W IHRDPK W D  S
Sbjct: 363 YLQNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALS 422

Query: 429 FRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEIDL 488
           F+PERFE  K  EVNK++ FG+GRRACPG  LA R VG  +  LIQCFEW++    ++D+
Sbjct: 423 FKPERFE--KEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDM 482

Query: 489 SQGNGLTMPKAQPLEAMCK 503
            +G G+TMP   PL AMCK
Sbjct: 483 MEGKGITMPMKIPLRAMCK 491

BLAST of MS008722 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 487.6 bits (1254), Expect = 1.7e-136
Identity = 233/490 (47.55%), Postives = 333/490 (67.96%), Query Frame = 0

Query: 22  LIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQMLQNLSQKYGPIVSLSFGFRP 81
           L  +F   ++    KNLPPGPPSLP+IG+LH L+ P H+  + LS+KYG + SL FG R 
Sbjct: 16  LFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRL 75

Query: 82  VVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVGATAYGQHWRIMRRIATTDL 141
           VVVVSS S  Q+CFTKND++ A+RPR +SGK + YNYT +G+T+YG+HWR +RRI   D+
Sbjct: 76  VVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDV 135

Query: 142 LSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSRLTELSFNIALRMMTGKRYFG 201
           LS HR+NSF  +R++E +  +  L   +      +E+ SRL +++FN  +RM++GKRY+G
Sbjct: 136 LSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYG 195

Query: 202 --VDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQGFRKRLWKVWAGADGFA 261
              D  D +E  + + ++ ++  LSGA+   DF+ +L+ +D++   KRL  +    D F 
Sbjct: 196 EDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFL 255

Query: 262 QSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIKGQILTLLAAGTDTT 321
           + LI   R +K        E    MID +L+LQDS P YY D IIKG  L +L AGTD++
Sbjct: 256 RGLIEEHRTKK--------ERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSS 315

Query: 322 AGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNLPYLQAIINETYRMFPAL 381
           A T+EW MS LLNHP  +KK   E++ +VG+ RLV+E+DL  L YL+ +INET R++   
Sbjct: 316 AVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPA 375

Query: 382 PILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPTSFRPERFENGKIVEVNK 441
           P+L+P  +S++C I GY VP+ T++++NAWAIHRDP++W + T+F+PERFE  K  E+ K
Sbjct: 376 PLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE--KKGELEK 435

Query: 442 LLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEIDLSQGNGLTMPKAQPLEA 501
           L+ FGMGRRACPG GLA R + + LA LIQCF+W  I  ++IDL++ +G T+ K  PL+A
Sbjct: 436 LIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLKA 494

Query: 502 MCKPRAPMIN 510
           MCK R P+IN
Sbjct: 496 MCKSR-PVIN 494

BLAST of MS008722 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 1.1e-135
Identity = 244/500 (48.80%), Postives = 326/500 (65.20%), Query Frame = 0

Query: 10  FHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQMLQNLSQKY 69
           F+  + ++L F +I +  F       KNLPPGP  LP+IG+LH L++P H     LSQKY
Sbjct: 4   FYLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKY 63

Query: 70  GPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVGATAYGQH 129
           G I SL FG R VVVVSS + AQECFTKNDI+ A+RP  ++GK + YN T V  + YG H
Sbjct: 64  GQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDH 123

Query: 130 WRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSRLTELSFNI 189
           WR +RRI + ++LS+HRLNSF+ +R++E+   ++ L + +      VE+R   +E++FN 
Sbjct: 124 WRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNT 183

Query: 190 ALRMMTGKRYFG--VDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQGFRKR 249
            +RM++GKRY+G   DV D EE R  + ++K+V  L GA+   DFL  L+  D+ G  KR
Sbjct: 184 IMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKR 243

Query: 250 LWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIKGQ 309
           L K+    D F Q LI+  R  K             MID +L+ Q S P YY D IIKG 
Sbjct: 244 LKKISKRTDAFLQGLIDEHRFGK--------RNSNTMIDHLLTQQQSQPEYYTDQIIKGL 303

Query: 310 ILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNLPYLQA 369
           ++ +L AGTDT++ TIEW MS LLNHP  MKKA +E++ ++G  R V+E D+  LPYLQ+
Sbjct: 304 MVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQS 363

Query: 370 IINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPTSFRPE 429
           I+ ET R+  A P+LVP  SSED ++ GYN+P+ T+L+VNAW IHRDP +W DPT F+PE
Sbjct: 364 IVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPE 423

Query: 430 RFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEIDLSQGN 489
           RFE  K  EVNKLL FG+GRRACPG  L+ R  GL L  LIQCFEW +IG  +ID+ +  
Sbjct: 424 RFE--KEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAK 483

Query: 490 GLTMPKAQPLEAMCKPRAPM 508
           G+T  K   L AMCK R P+
Sbjct: 484 GITAGKKTSLNAMCKVRHPL 493

BLAST of MS008722 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 472.2 bits (1214), Expect = 7.3e-132
Identity = 232/508 (45.67%), Postives = 337/508 (66.34%), Query Frame = 0

Query: 12  FLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQMLQNLSQKYGP 71
           + I L LLF +I  +KF   K    NLPPGPPS P +GHLHL++ P H++LQ  S +YGP
Sbjct: 3   YFILLPLLFLVIS-YKFLYSKTQRFNLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGP 62

Query: 72  IVSLSFGFRPVVVVSSPSAAQECFT-KNDIIFASRPRMISGKILNYNYTAVGATAYGQHW 131
           I SL FG R VVV++SPS AQE FT +NDI+ +SRP  ++ K + YN+T VG   YG HW
Sbjct: 63  IFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHW 122

Query: 132 RIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQIN-----VRVEIRSRLTEL 191
           R +RRI + ++LS+HRL +F ++R++E+   L  L R T   N       +E+   L++L
Sbjct: 123 RNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDL 182

Query: 192 SFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQGFR 251
           +FN  +RM+TGKRY+G DV + EE    KK++  + + SGA++ AD+L +LKL   + F 
Sbjct: 183 TFNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPILKLFGNK-FE 242

Query: 252 KRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIK 311
           K +  +    D   Q L++  R          ++E   M++ ++SLQ   P YY D IIK
Sbjct: 243 KEVKAIGKSMDDILQRLLDECRR---------DKEGNTMVNHLISLQQQQPEYYTDVIIK 302

Query: 312 GQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNLPYL 371
           G +++++ AGT+T+A T+EW M+ LL +P  ++KA SEI++ +G+ RL++E+D+  LPYL
Sbjct: 303 GLMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYL 362

Query: 372 QAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPTSFR 431
           Q +++ET+R+FP  P L+PR  ++D  I GY+VP+ T+++VNAWAIHRDP++WE+P  F 
Sbjct: 363 QNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFN 422

Query: 432 PERFEN--GKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEIDL 491
           P+R+ +  G    V KL+PFG GRR CPGAGL  R+V LAL +LIQCFEW+ +   E+D+
Sbjct: 423 PDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDM 482

Query: 492 SQGNGLTMPKAQPLEAMCKPRAPMINLL 512
           S+  GL M K  PL AMC+PR  M  LL
Sbjct: 483 SESTGLGMRKMDPLRAMCRPRPIMSKLL 499

BLAST of MS008722 vs. ExPASy TrEMBL
Match: A0A6J1C9L7 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009467 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 5.5e-276
Identity = 467/512 (91.21%), Postives = 490/512 (95.70%), Query Frame = 0

Query: 3   MEETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQML 62
           MEETLQF  FLIPL LLF LIHVF FKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQML
Sbjct: 1   MEETLQFSRFLIPLALLFALIHVFNFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQML 60

Query: 63  QNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVG 122
           QNLS+KYGPI+SLS GFR VVVVSSPSAAQECFTKNDI+FA+RPRMISGKILNYNYT+VG
Sbjct: 61  QNLSKKYGPILSLSLGFRSVVVVSSPSAAQECFTKNDIVFANRPRMISGKILNYNYTSVG 120

Query: 123 ATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSRL 182
           ATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQ+EVKLWLKNLY+TTGQ NV+VEIR++L
Sbjct: 121 ATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQQEVKLWLKNLYQTTGQNNVQVEIRTKL 180

Query: 183 TELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQ 242
           TELSFNIALRMMTGKRYFGVDVEDSEEGR+LKKVMK+V LLSGASYPADFLSVLKLIDYQ
Sbjct: 181 TELSFNIALRMMTGKRYFGVDVEDSEEGRKLKKVMKEVSLLSGASYPADFLSVLKLIDYQ 240

Query: 243 GFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDH 302
           GFRKRLWKVWAGADGFAQSLI+GRRERK SCSFSPEE RKNMIDTMLSLQDSDP YYKDH
Sbjct: 241 GFRKRLWKVWAGADGFAQSLIDGRRERKASCSFSPEESRKNMIDTMLSLQDSDPGYYKDH 300

Query: 303 IIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNL 362
           IIKGQILTLLAAGTDTTAGTIEWGMSLLLNH T MKK WSEIN+YVGEQRLVEEADL NL
Sbjct: 301 IIKGQILTLLAAGTDTTAGTIEWGMSLLLNHRTIMKKVWSEINEYVGEQRLVEEADLTNL 360

Query: 363 PYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPT 422
           P+LQAIINET R+FPALPILVPRESSEDCTI GY++PKGTMLVVN WAIHRDPKVW+DPT
Sbjct: 361 PFLQAIINETNRLFPALPILVPRESSEDCTIGGYDIPKGTMLVVNVWAIHRDPKVWQDPT 420

Query: 423 SFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEID 482
           SF+PERFENG++VE NKLLPFGMGRRACPG+GLAHRVVGLALATLIQCFEWDKIGPNEID
Sbjct: 421 SFQPERFENGEVVEANKLLPFGMGRRACPGSGLAHRVVGLALATLIQCFEWDKIGPNEID 480

Query: 483 LSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
           L QGNGLTMPKA PLEAMCKPRAPMINLLSQL
Sbjct: 481 LFQGNGLTMPKAHPLEAMCKPRAPMINLLSQL 512

BLAST of MS008722 vs. ExPASy TrEMBL
Match: A0A6J1C4W6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111008503 PE=3 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.0e-266
Identity = 453/512 (88.48%), Postives = 473/512 (92.38%), Query Frame = 0

Query: 3   MEETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQML 62
           MEE LQF  FL+PL LLF LIHVF FK KKVSSKNLPP PPSLPVIGHLHLLREPFHQML
Sbjct: 1   MEEILQFSRFLVPLALLFALIHVFNFKFKKVSSKNLPPXPPSLPVIGHLHLLREPFHQML 60

Query: 63  QNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVG 122
           QNLSQ+YGPI+SLS GFR VVVVSSP AAQECFTKNDI+FA+RPRMISGKILNYNYT VG
Sbjct: 61  QNLSQRYGPILSLSLGFRSVVVVSSPIAAQECFTKNDIVFANRPRMISGKILNYNYTTVG 120

Query: 123 ATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSRL 182
           ATAYGQHWRIMRRIATT+LLSTHRL SFINLRQEEVKLWLKNLYR TGQ NVRVEIRSRL
Sbjct: 121 ATAYGQHWRIMRRIATTELLSTHRLISFINLRQEEVKLWLKNLYRITGQNNVRVEIRSRL 180

Query: 183 TELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQ 242
           TELSFNI LRMMTGKRYFGV+V+DSEE RELKKVMK+V LLSG SYPADFLSVL+LIDYQ
Sbjct: 181 TELSFNIVLRMMTGKRYFGVEVKDSEEERELKKVMKEVSLLSGMSYPADFLSVLRLIDYQ 240

Query: 243 GFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDH 302
           GFRKRLWKVWAGADGF Q LI+GRRE K SCSFS EE  KNMIDTMLSLQDSDP YYKDH
Sbjct: 241 GFRKRLWKVWAGADGFVQXLIDGRREMKASCSFSLEENWKNMIDTMLSLQDSDPDYYKDH 300

Query: 303 IIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNL 362
           IIKGQILTLL AGTDTTAGTIEWGMSLLLNHPT MKKAWSEINDYVGEQRLVEEADL NL
Sbjct: 301 IIKGQILTLLVAGTDTTAGTIEWGMSLLLNHPTIMKKAWSEINDYVGEQRLVEEADLANL 360

Query: 363 PYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPT 422
           PYLQAIINETYR+FPALPILVPRESSEDCTI GY+VPKGTMLVVN WAIHRDP+VW+DPT
Sbjct: 361 PYLQAIINETYRLFPALPILVPRESSEDCTIGGYDVPKGTMLVVNMWAIHRDPQVWQDPT 420

Query: 423 SFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEID 482
            F+PERFENG++VEVNKLLPFGMGRRACPGAGLAHRVVGL LATLIQCFEWD+I PNEID
Sbjct: 421 RFQPERFENGEVVEVNKLLPFGMGRRACPGAGLAHRVVGLTLATLIQCFEWDRIDPNEID 480

Query: 483 LSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
           L QGNGLTMPKA PLEAMCKPR PMI+LLS L
Sbjct: 481 LXQGNGLTMPKAHPLEAMCKPRGPMISLLSHL 512

BLAST of MS008722 vs. ExPASy TrEMBL
Match: A0A6J1FJ78 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111445861 PE=3 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 1.5e-228
Identity = 398/516 (77.13%), Postives = 454/516 (87.98%), Query Frame = 0

Query: 1   ASMEETLQFFHFLIP-LTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFH 60
           ++MEETL F +F +P L L+  L+ +FKF  +K   KNLPPGPPSLPVIGHLHLL+EPFH
Sbjct: 3   STMEETLLFSNFEVPLLVLILALLLIFKF--RKAEHKNLPPGPPSLPVIGHLHLLKEPFH 62

Query: 61  QMLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYT 120
           QMLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YT
Sbjct: 63  QMLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYT 122

Query: 121 AVGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIR 180
           AVGATAYGQHWRIMRRIATT+LLST+RLN+ IN+RQ+EVKLWLK+LYR +G+ NVRVEIR
Sbjct: 123 AVGATAYGQHWRIMRRIATTELLSTYRLNALINIRQQEVKLWLKSLYRISGKNNVRVEIR 182

Query: 181 SRLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKL 240
           S+LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYPADFLSVLKL
Sbjct: 183 SKLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKL 242

Query: 241 IDYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHY 300
           +DYQGFRKRL KV    D FAQSLI+ RR +K     + + ERK MID++LS+Q  +P Y
Sbjct: 243 VDYQGFRKRLAKVRVDGDAFAQSLIDERRNKK-----ADDNERKTMIDSLLSMQQMEPEY 302

Query: 301 YKDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEAD 360
           YKD IIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG++RLVEE D
Sbjct: 303 YKDDIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMD 362

Query: 361 LVNLPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVW 420
           L  L YLQ IINETYR+FPALPILVPRESSEDCTI G++VPKGTMLVVNAWAIHRDPKVW
Sbjct: 363 LNQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPKGTMLVVNAWAIHRDPKVW 422

Query: 421 EDPTSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGP 480
           EDP SFRPERF+ G+ VEV+KLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEW+++  
Sbjct: 423 EDPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDN 482

Query: 481 NEIDLSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
            E+DL+ G GLTMPKA+PL AM KPRAP+I+LLSQL
Sbjct: 483 KEVDLTPGIGLTMPKARPLVAMFKPRAPIIHLLSQL 511

BLAST of MS008722 vs. ExPASy TrEMBL
Match: A0A6J1J4B3 (isoflavone 3'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111481120 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 6.2e-227
Identity = 394/515 (76.50%), Postives = 445/515 (86.41%), Query Frame = 0

Query: 1   ASMEETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQ 60
           ++MEETL  F     L L+  L  +  FK +K   KNLPPGPPSLPVIGHLHLL+EPFHQ
Sbjct: 3   STMEETLLLFSNFEVLLLILVLALLMIFKFRKADHKNLPPGPPSLPVIGHLHLLKEPFHQ 62

Query: 61  MLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTA 120
           MLQ+LSQKYGPI+SLS GFRPVVVVSSP+AAQECF+KNDIIFASRPRMISGKILNY+YTA
Sbjct: 63  MLQDLSQKYGPILSLSLGFRPVVVVSSPAAAQECFSKNDIIFASRPRMISGKILNYDYTA 122

Query: 121 VGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRS 180
           VGATAYGQHWRIMRRIATT+LLSTHRLN+ IN+RQ+EVKLWLK+LYR TG+ NVRVEIRS
Sbjct: 123 VGATAYGQHWRIMRRIATTELLSTHRLNALINIRQQEVKLWLKSLYRITGKNNVRVEIRS 182

Query: 181 RLTELSFNIALRMMTGKRYFGVD-VEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLI 240
           +LTELSFNIAL +M GKRYFG + V+DSEE RELK  MKQVPLLSGASYPADFLSVLKL+
Sbjct: 183 KLTELSFNIALMLMVGKRYFGDEAVDDSEEARELKNTMKQVPLLSGASYPADFLSVLKLV 242

Query: 241 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 300
           DYQGFRK+L KV    D FAQSLI+ RR +K     +   ERK MID++LS+Q  +P YY
Sbjct: 243 DYQGFRKKLAKVRVDGDAFAQSLIDERRNKK-----ADHNERKTMIDSLLSMQQMEPEYY 302

Query: 301 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADL 360
           KDHIIKGQ LTLLAAGTDTTAGT+EW +SLLLNHPTAMKKAW+EIN++VG++RLVEE DL
Sbjct: 303 KDHIIKGQFLTLLAAGTDTTAGTLEWALSLLLNHPTAMKKAWTEINEHVGDERLVEEMDL 362

Query: 361 VNLPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWE 420
             L YLQ IINETYR+FPALPILVPRESSEDCTI G++VP GTMLVVNAWAIHRDPKVWE
Sbjct: 363 NQLHYLQGIINETYRLFPALPILVPRESSEDCTIGGFDVPIGTMLVVNAWAIHRDPKVWE 422

Query: 421 DPTSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPN 480
           DP SFRPERF+ G+ VEV+KLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEW+++   
Sbjct: 423 DPNSFRPERFKEGESVEVSKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWERLDNK 482

Query: 481 EIDLSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
           E+DL+ G GLTMPK+ PL AM KPRAP+ +LLSQL
Sbjct: 483 EVDLTPGIGLTMPKSSPLVAMFKPRAPITHLLSQL 512

BLAST of MS008722 vs. ExPASy TrEMBL
Match: A0A5D3D9B5 (Isoflavone 2'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002940 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 4.2e-207
Identity = 373/525 (71.05%), Postives = 423/525 (80.57%), Query Frame = 0

Query: 3   MEETLQFF----HFLIPLTLLF--TLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLRE 62
           ME+   FF    HF  PL  +F  TL  + KF+  + +   LPP PPSLPVIGHLHLL+E
Sbjct: 3   MEQQTSFFFFFSHFNFPLLAIFLTTLFLISKFRRSEHTKLLLPPSPPSLPVIGHLHLLKE 62

Query: 63  PFHQMLQNLSQKYGPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNY 122
           PFHQMLQ+LS KYGP+ SL+ GFRP+VVVSSPSA  ECFTKNDI+FASRPRM+SGKILNY
Sbjct: 63  PFHQMLQDLSLKYGPVFSLTLGFRPLVVVSSPSAVHECFTKNDIVFASRPRMLSGKILNY 122

Query: 123 NYTAVGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTG----QI 182
           +YTAVGATAYGQHWRIMRRIATT+LLST RLN F  LR+EEVKLW+K LY +      Q 
Sbjct: 123 DYTAVGATAYGQHWRIMRRIATTELLSTKRLNDFSKLREEEVKLWVKRLYGSVTIREVQK 182

Query: 183 NVRVEIRSRLTELSFNIALRMMTGKRYFGVDVE--DSEEGRELKKVMKQVPLLSGASYPA 242
             RV +RS+LTELSFNIAL +  G+RYFG +VE  +SEE +ELK+ MKQVPLLSGASYPA
Sbjct: 183 GGRVGLRSKLTELSFNIALMLTAGRRYFGEEVEEGESEEAKELKETMKQVPLLSGASYPA 242

Query: 243 DFLSVLKLIDYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLS 302
           DFLSVLKLID+QGFRKRL KV    D FAQSL++ RRE K S       ERK MID MLS
Sbjct: 243 DFLSVLKLIDFQGFRKRLAKVRVDGDAFAQSLVDERRELKGSWC-----ERKTMIDAMLS 302

Query: 303 LQDSDPHYYKDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGE 362
           LQ+SDP YYKD IIKGQ LTLLAAGTDT A T+EW MSLLLNHP  M KAW+EIN  VG+
Sbjct: 303 LQESDPEYYKDDIIKGQFLTLLAAGTDTIASTLEWAMSLLLNHPIVMNKAWTEINQVVGD 362

Query: 363 QRLVEEADLVNLPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWA 422
           QRLV EAD  NL YLQ+IINETYR+FPALPILVPRESSEDCTI G+++PKGTMLVVNAWA
Sbjct: 363 QRLVREADTNNLHYLQSIINETYRLFPALPILVPRESSEDCTIGGFDIPKGTMLVVNAWA 422

Query: 423 IHRDPKVWEDPTSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQC 482
           +HR+P+VWEDP  F+PERF+ G+ VEV+KLLPFGMGRRACPGAGLAHRVV LALATLIQC
Sbjct: 423 VHRNPEVWEDPNCFKPERFKQGETVEVSKLLPFGMGRRACPGAGLAHRVVSLALATLIQC 482

Query: 483 FEWDKI-GPNEIDLSQGNGLTMPKAQPLEAMCKPRAPMINLLSQL 515
           FEW K+    ++DL  G GLTMPKA PLEAMCKPRA + +LLSQL
Sbjct: 483 FEWGKLDNKKDVDLCPGIGLTMPKAIPLEAMCKPRASVTHLLSQL 522

BLAST of MS008722 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 482.6 bits (1241), Expect = 3.8e-136
Identity = 242/512 (47.27%), Postives = 350/512 (68.36%), Query Frame = 0

Query: 5   ETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPP-SLPVIGHLHLLREPFHQMLQ 64
           +TL F    + L+L++ +      KLK+    NLPP P  SLPVIGHL LL+ P H+   
Sbjct: 4   KTLIFSILFVVLSLIYLI-----GKLKR--KPNLPPSPAWSLPVIGHLRLLKPPIHRTFL 63

Query: 65  NLSQKY--GPIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAV 124
           +LSQ     PI SL  G R V V SS S A+ECFTKND++ A+RP  I  K + Y+YT +
Sbjct: 64  SLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTM 123

Query: 125 GATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSR 184
            A +YG HWR +RRI + ++ S HRLNSF+++R++E++  +  L R   Q  V+V+++S 
Sbjct: 124 IAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSM 183

Query: 185 LTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDY 244
           L++L+FN  LRM+ GKRY+G  VED  E + +++++  V   +GA    D+L VL+L+  
Sbjct: 184 LSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV-- 243

Query: 245 QGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKD 304
             +  R+ K+    D F Q L++ +RE K        E+   MID +L+LQ+S P Y+ D
Sbjct: 244 SDYETRVKKLAGRLDEFLQGLVDEKREAK--------EKGNTMIDHLLTLQESQPDYFTD 303

Query: 305 HIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVN 364
            IIKG +L L+ AGTDT+A T+EW +S +LNHP  + KA  EI+  +G  RL++E+D+ N
Sbjct: 304 RIIKGNMLALILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISN 363

Query: 365 LPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDP 424
           LPYLQ I++ET R++PA P+L+P  +SEDC + GY++P+GT+L+ N WAIHRDP++W+DP
Sbjct: 364 LPYLQNIVSETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDP 423

Query: 425 TSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEI 484
            SF+PERFE  K  E  KL+PFG+GRRACPG+GLAHR++ L L +LIQC EW+KIG  E+
Sbjct: 424 MSFKPERFE--KEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG-EEV 483

Query: 485 DLSQGNGLTMPKAQPLEAMCKPRAPMINLLSQ 514
           D+S+G G+TMPKA+PLEAMC+ R  ++ + ++
Sbjct: 484 DMSEGKGVTMPKAKPLEAMCRARPSVVKIFNE 495

BLAST of MS008722 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 476.1 bits (1224), Expect = 3.6e-134
Identity = 244/503 (48.51%), Postives = 341/503 (67.79%), Query Frame = 0

Query: 12  FLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPP-SLPVIGHLHLLREPFHQMLQNLSQKYG 71
           FL   TL   L+   K KL      NLPP P  SLPVIGHLHLL+ P H+   +LS+  G
Sbjct: 11  FLSLFTLAIFLLTRSKRKL------NLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIG 70

Query: 72  --PIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYTAVGATAYGQ 131
             P+  L  G R V V+SS S A+ECFTKND++ A+RP+    K L YN T + + +YG 
Sbjct: 71  NAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGD 130

Query: 132 HWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIRSRLTELSFN 191
           HWR +RRIA  ++ STHRLNSF+ +R++E++  + +L R +    V VE+++ LT L+ N
Sbjct: 131 HWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASN 190

Query: 192 IALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQGFRKRL 251
             +RM+ GKRYFG   ED+++ + +K ++ +    +GA  P D+LS+L+ +    + KR+
Sbjct: 191 TTIRMLAGKRYFG---EDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRI 250

Query: 252 WKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIKGQI 311
             +    D F Q L++ +R  K        E+ + MID +L+LQD  P YY D IIKG I
Sbjct: 251 KNLGNRFDTFLQKLVDEKRAEK--------EKGETMIDHLLALQDIQPDYYTDVIIKGII 310

Query: 312 LTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNLPYLQAI 371
           LTL+ AGTDT++ T+EW MS LLNHP  +KKA  EI++ VG  RLV+E+D+VNL YLQ+I
Sbjct: 311 LTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSI 370

Query: 372 INETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPTSFRPER 431
           + ET RM+PA+P+L+P  SSEDC + GY++P GTM++ NAWA+HRDP+VWEDP  F+PER
Sbjct: 371 VLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPER 430

Query: 432 FENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEIDLSQGNG 491
           FE  K  E  KL+ FGMGRRACPGAGLAHR++  AL +L+QCFEW+++G + +D+++  G
Sbjct: 431 FE--KEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKG 490

Query: 492 LTMPKAQPLEAMCKPRAPMINLL 512
            T+PKA PL AMCK R+ +  L+
Sbjct: 491 ATLPKAIPLRAMCKARSIVDKLI 492

BLAST of MS008722 vs. TAIR 10
Match: AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2 )

HSP 1 Score: 472.2 bits (1214), Expect = 5.2e-133
Identity = 232/508 (45.67%), Postives = 337/508 (66.34%), Query Frame = 0

Query: 12  FLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPPSLPVIGHLHLLREPFHQMLQNLSQKYGP 71
           + I L LLF +I  +KF   K    NLPPGPPS P +GHLHL++ P H++LQ  S +YGP
Sbjct: 3   YFILLPLLFLVIS-YKFLYSKTQRFNLPPGPPSRPFVGHLHLMKPPIHRLLQRYSNQYGP 62

Query: 72  IVSLSFGFRPVVVVSSPSAAQECFT-KNDIIFASRPRMISGKILNYNYTAVGATAYGQHW 131
           I SL FG R VVV++SPS AQE FT +NDI+ +SRP  ++ K + YN+T VG   YG HW
Sbjct: 63  IFSLRFGSRRVVVITSPSLAQESFTGQNDIVLSSRPLQLTAKYVAYNHTTVGTAPYGDHW 122

Query: 132 RIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQIN-----VRVEIRSRLTEL 191
           R +RRI + ++LS+HRL +F ++R++E+   L  L R T   N       +E+   L++L
Sbjct: 123 RNLRRICSQEILSSHRLINFQHIRKDEILRMLTRLSRYTQTSNESNDFTHIELEPLLSDL 182

Query: 192 SFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLIDYQGFR 251
           +FN  +RM+TGKRY+G DV + EE    KK++  + + SGA++ AD+L +LKL   + F 
Sbjct: 183 TFNNIVRMVTGKRYYGDDVNNKEEAELFKKLVYDIAMYSGANHSADYLPILKLFGNK-FE 242

Query: 252 KRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYYKDHIIK 311
           K +  +    D   Q L++  R          ++E   M++ ++SLQ   P YY D IIK
Sbjct: 243 KEVKAIGKSMDDILQRLLDECRR---------DKEGNTMVNHLISLQQQQPEYYTDVIIK 302

Query: 312 GQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADLVNLPYL 371
           G +++++ AGT+T+A T+EW M+ LL +P  ++KA SEI++ +G+ RL++E+D+  LPYL
Sbjct: 303 GLMMSMMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYL 362

Query: 372 QAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWEDPTSFR 431
           Q +++ET+R+FP  P L+PR  ++D  I GY+VP+ T+++VNAWAIHRDP++WE+P  F 
Sbjct: 363 QNVVSETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFN 422

Query: 432 PERFEN--GKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPNEIDL 491
           P+R+ +  G    V KL+PFG GRR CPGAGL  R+V LAL +LIQCFEW+ +   E+D+
Sbjct: 423 PDRYNDGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDM 482

Query: 492 SQGNGLTMPKAQPLEAMCKPRAPMINLL 512
           S+  GL M K  PL AMC+PR  M  LL
Sbjct: 483 SESTGLGMRKMDPLRAMCRPRPIMSKLL 499

BLAST of MS008722 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 471.5 bits (1212), Expect = 8.8e-133
Identity = 238/505 (47.13%), Postives = 339/505 (67.13%), Query Frame = 0

Query: 3   MEETLQFFHFLIPLTLLFTLIHVFKFKLKKVSSKNLPPGPP-SLPVIGHLHLLREPFHQM 62
           ME  +  F F   +  L  LI   K KL      NLPP P  +LPVIGHL LL+ P H++
Sbjct: 1   MEALMLIFTFCFIVLSLIFLIGRIKRKL------NLPPSPAWALPVIGHLRLLKPPLHRV 60

Query: 63  LQNLSQKYG--PIVSLSFGFRPVVVVSSPSAAQECFTKNDIIFASRPRMISGKILNYNYT 122
             ++SQ  G  PI+SL  G R + VVSS S A+ECFTKND+I A+R   IS K ++Y  +
Sbjct: 61  FLSVSQSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNS 120

Query: 123 AVGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINVRVEIR 182
            V + +Y +HWR +RRI   ++ S HRLNSF ++R++E++  +  L R +     +VE++
Sbjct: 121 TVVSASYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMK 180

Query: 183 SRLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLSVLKLI 242
           S  ++L+FN  +RM+ GK Y+G   ED  E + ++ ++ +    SG    AD++ +L  I
Sbjct: 181 SMFSDLTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWI 240

Query: 243 DYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDSDPHYY 302
            Y     R+ K+    D F Q L++ +RE K       E++   M+D +L LQ++ P YY
Sbjct: 241 TYS--ETRIKKLAGRLDEFLQGLVDEKREGK-------EKKENTMVDHLLCLQETQPEYY 300

Query: 303 KDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLVEEADL 362
            D IIKG +L+L+A GTDTTA T+EW +S LLN+P  + KA  EI+  +G  RL+EE+D+
Sbjct: 301 MDRIIKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDI 360

Query: 363 VNLPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRDPKVWE 422
            NLPYLQ I++ET R++PA P+L+P  +S+DC + GY++P+GTML+ NAWAIHRDP +W+
Sbjct: 361 PNLPYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWD 420

Query: 423 DPTSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWDKIGPN 482
           DPTSF+PERFE  K  E  KL+PFG+GRRACPG+GLA R+V L+L +LIQCFEW++IG  
Sbjct: 421 DPTSFKPERFE--KEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEE 480

Query: 483 EIDLSQGNGLTMPKAQPLEAMCKPR 505
           E+D+++G GLTMPKA+PLEAMC+ R
Sbjct: 481 EVDMTEGPGLTMPKARPLEAMCRAR 488

BLAST of MS008722 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 466.5 bits (1199), Expect = 2.8e-131
Identity = 244/518 (47.10%), Postives = 338/518 (65.25%), Query Frame = 0

Query: 3   MEETLQFFHFLIPLTLLFTLIH-VFKFKLKKVSSKNLPPGPPS-LPVIGHLHLLREPFHQ 62
           MEET    +  + L  +F+LI  +  FK  K   +NLPP PP  LP+IGHL LL+ P H+
Sbjct: 1   MEET----NIRVVLYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHR 60

Query: 63  MLQNLSQKY-----GPIVSLSFGFRPVVVVSSPS-AAQECFTKNDIIFASRPRMISGKIL 122
            L++ S+       G ++SL  G R V VVSS   AA+ECF KND++ A+RP++I GK +
Sbjct: 61  TLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHV 120

Query: 123 NYNYTAVGATAYGQHWRIMRRIATTDLLSTHRLNSFINLRQEEVKLWLKNLYRTTGQINV 182
            YN T + A  YG HWR +RR+ T ++ STHRLN F+ +R +EV+  +  L R  G    
Sbjct: 121 GYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKT 180

Query: 183 RVEIRSRLTELSFNIALRMMTGKRYFGVDVEDSEEGRELKKVMKQVPLLSGASYPADFLS 242
            VE++  L +L+FN  +RMMTGKRY+G +  D EE + ++K++  V   + +    D++ 
Sbjct: 181 VVELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVP 240

Query: 243 VLKLIDYQGFRKRLWKVWAGADGFAQSLINGRRERKVSCSFSPEEERKNMIDTMLSLQDS 302
           +L+L  +  +  R+ K+    D F Q LI+ +R          +E    MID +L LQ S
Sbjct: 241 ILRL--FSSYENRVKKLGEETDKFLQGLIDDKR--------GQQETGTTMIDHLLVLQKS 300

Query: 303 DPHYYKDHIIKGQILTLLAAGTDTTAGTIEWGMSLLLNHPTAMKKAWSEINDYVGEQRLV 362
           D  YY D IIKG IL ++ AGT+T+A T+EW +S LLNHP  + KA  EI++ VG  RL+
Sbjct: 301 DIEYYTDQIIKGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLI 360

Query: 363 EEADLVNLPYLQAIINETYRMFPALPILVPRESSEDCTIEGYNVPKGTMLVVNAWAIHRD 422
           EEADL  LPYL+ I+ ET R+ PA P+LVP  +SEDC I  Y++P+GT L+VNAWAIHRD
Sbjct: 361 EEADLSELPYLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRD 420

Query: 423 PKVWEDPTSFRPERFENGKIVEVNKLLPFGMGRRACPGAGLAHRVVGLALATLIQCFEWD 482
           P  W+DP SF+PERFE  K  E  KLL FG+GRRACPG+GLA R+VGLAL +LIQCFEW+
Sbjct: 421 PNTWDDPDSFKPERFE--KEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWE 480

Query: 483 KIGPNEIDLSQGNGLTMPKAQPLEAMCKPRAPMINLLS 513
           ++G  E+D+ +G G T+PKA PL+A+CK R  +  ++S
Sbjct: 481 RVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHKIIS 502