MS008717 (gene) Bitter gourd (TR) v1

Overview
NameMS008717
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionaspartic proteinase-like protein 2
Locationscaffold4: 3276851 .. 3282146 (-)
RNA-Seq ExpressionMS008717
SyntenyMS008717
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCGGGGAGGGCATTTTACCGGGTCCACCCTGCGTCAATCTCCGCCCTCTCTCTTCCGTCTGCTCCGTCGCGTGCGGCCACCGGCGCACCATGTCTCCGTCTGGCGCCAATTCACCTTCTGCTATTGTCATCTATTGCCTAGTCGGTTTCAATCTGTTGCGCACGGTTTTCTCCTCATCTGTGGACTCAAGAGATTTCGATTATCAGCAGCGCCCCGTGATTCTTCCTCTCTACATCTCGACTACGAATTCCACTCATCACCGCGTCTTGAACCGTGATCATCGTTTCCGGCACCTGCAGAAATTGGACCAGCCCCACTCCTCAACTGCTCGAATGAGGCTGCACGATGACCTCCTTACCAACGGGTTCGTCTTTCAGAGTTTTTCGGCTTTTGGTTCTGTTATTAGATCGTTTACATGTTTATCAACAATTTGAGGGTTCTGGGACGTCGATATTTATAGGTATTACACGACGCGTCTATGGATTGGGACTCCTCCGCAAGAATTCGCTCTTATTGTGGACACCGGAAGTACAGTGACCTACGTTCCATGCTCTAACTGCGAAGAGTGCGGGAACCATCAGGTTTCTTTCGGTTGATTGCTGATCTGCGTTGTTTTTCAGTTCTGCTGTCTATAGCTAAATATCTGCAACTCGTTTTCTGGGTTCGAATTTGGGCATACAGATACTGCTATTGTTATGTCTTGAGTTCTGTAACTACTGAACACGATGTAAGCACGGGATCTTAGAACTGGGCATGGTCGGAATTGCTGATGAAGGAAAACCTAGAAATTAAAGATCATCCATTATGGGATCATGGTTTCCCACGTGTTTTTCTTCGGAAGTAATTCTCTGTATATGGATCCACAAAGATAAAACGTAATTGAATTTTTCCTATGAAAATATTTAATCTCAGGGGTAGGAAGCCTTCATAACATTCCTTCATGTGAGAATTCAAATATCATTCCTTCAAGTAACTTTGCATATATGATATATGTTTAGCATGATAGTAGTTGTTAGGTGCTGCATTGGAAGATCATATCGGATTATTCATAGTGAATATGATGGTGAAGATGACTAAAACTCCTAGCAAAGCTTATGCATGTTCCATTAAATAAGGAGCTTTGGATTAATTGTCAAAGGAAGTTCAGATAGTTTGAACTATTGATTACTCTATTTCTGCTCGATCTAAGTTTTTAAAACAAGTTTTCACTTGATTTATTTTTAGAACCATGCTATTTTTAGGATCCAAAGTTCCAACCAGAGTTGTCAAGCACATATCAACCTGTGAAGTGCAATATCAATTGTAACTGTGATGACAAGGGAGTCCAGTGTACATATGAGAGACGGTATGCAGAAATGAGCACTAGCAGTGGTATGCTTGCTGAGGACATCATGTCATTTGGAAAGGAAAGTGAACTTGTACCCCAGCGTGCTGTATTTGGTTGTGAAACTATGGAAACTGGTGACCTTTATACCCAACGTGCTGATGGGATTATGGGTTTGGGCCGTGGTTCACTCAGTGTGATGGACCAGCTTGCCAACAAGGGTGTTGTGAGCGATTCATTTTCGTTATGTTACGGTGGGATGGATGTTGGTGGGGGTGCAATGGTTCTTGGTGGGATCTCTTCACCCCCTGGTATGGTATTTACCCACTCGGACCCATCTCGAAGGTATGGTAGTTTTGAATCTGCCTTTCTAGATTTTTCTTCACTGGATTATATTTGTGTGCATCTCATAGATACAGTCTAGCTTTGGGATGGAAACAGCCCATATTATAATATTGAGTTGAAGGAAATACATGTTGCTGGGAAGCCATTGAAGTTGAATCCGAACACTTTTGATGGGAAGTATGGTACTGTCTTGGATAGTGGAACTACATATGCTTATTTTCCTGAAAAAGCCTACTATGCATTTAAAGATGCTGTAAGTTCTTCAACCTCTACATCTAATCGCAGGTTTTCTAATTTTAGATGGTCTTGCCTTCATCAAAAGCATATCGACATTGCTCTACTTATATTATTCCATCTGCTTAAATAATAATCTTGTGCCACACATCTCCTATTTCATTCGTTCTGCATTATAAAATGGAATTTACTAATACTCCAAAGCCATTGAAATTGTATAAATTCTTGTTCAGAAAGCTACTATATAGGATATAGAGATCTTCAATGTGCAATTCAGATTGATCGAGTATTTGAAAAATATCCAGTTCCCATATTTAATTCATGTGTTATTTTCTCATCTTTTACCTTTCTGAATTGCTTTGTATTTTCTGTACCTTTATATTTGATATTCCAAAGAAGACTTCTTGAATCAGTTCTTCATAATTGCATTTAGCTGATACAACTGTTTTTTAACTTTTACTTCTCAGTTGGACTCGAACTGCAAAACACATTCTGGAGACCTCTTCTATTTATTATTACCAAATATAATCCTCCTGCTTAGAGTTTCCCCATACCCGATGGTTGTTGTGTTCTAGTAGTTGGTTTAATAGATTTGACTCTTATCTTATGCTATTGACTTTAAGTTTAGGGACTGTTTCATGCAGGAACACATGTTTGGAGTTGGGTGTTGTTTTTGTTATTTGGAATGTAATGTTATAAATTTTTTGGATGACAAAATGTGATGCTATAAACTATGACCAGGGCTACAGGTCTTTTCTGAAATCCATGCTAAAATCTTCACAATCTATTCAGCAAAAAATAGAACTGCCCTTTTCATCAGTGCTCGTTTTATGAGGGGTTATTGTTAACTTCTTCATTCATGTGAACAACAAGTTTTGATTTCTTGACTGATACATTTTCATGCTAAATTCCATCTCCTGTTTTCCTCCACCGCTCTATTCTACAGCTTTTTACTATAAAACGTGGTGATTATAGTTAAATGATGTTTAATTTTCAGATAATGAAGAAAATTAATTTCCTGAAGCAAATTAGTGGTCCTGACCCGAATTTTAAGGATATTTGTTTTTCTGGTGCTGGAAGGTATTTCTTTATTTCTTGCTATAAGCACTTATATCTTAGGTTTACGTTTACCCCTCTAGCTGAATGTTTAGCATGGTCTTTTTTGTGCCAGGGATGTCAATGAACTTCCAAAAGTTTTTCCAGAGGTTGATTTTGTTTTTGCGAATGGTCAAAAAATTTCACTGTCTCCGGAGAATTACTTGTTCCGGGTATGCTTTTTTCTTTTTGCAATGTATTATTATCGTGATATGTTATTATTTGTAATTTTTTTAAAATTTTACATTAATGGTATATACTTCCGTCATTTTTCTTCTGATCAATCACTTCTGCAGCATACTAAGGTTAGTGGTGCCTATTGTCTGGGAATCTTTAAAAATGGGAATGATCAGACTACACTTTTGGGAGGTATGGTGAAGTTTCACTGGATTGTGGATAATTTTCTCTTGTGCTGTACATATTTTTTTTTTCTGTAGCACTTGTCGTGATTGGATATATATATATATATATTACAGTTTTATATTGCATGCTTTAAGAAAACTTAGGTGTTTAATAATCACCTTCCTCAGAGTTCATTAAACAAAATAGTAGTTCGTTAGAATAAAGCTGCATGTTTTCTTTTGTTGGCAGGAATTGTTGTTCGTAATACTCTTGTCACTTATGATCGGAAAAACACCTCAATCGGGTTTTGGAAGACTAATTGTTCTGAACTTTGGAAGAATCTGCATTATCTCGCTCCACCTCCAGCACCTTTACCTTCCTATGGTCAGAATACAAGTAAAGAAACTTCCCCACTTGGTTCTCCAACTGGGCTATTTCTTTCTGGTGAGGTTTAACCGTAAATTTGTATGAATTTTTGTGTTGCTTCTTAGTTTTGAATTATTTGTACAGAAGTTAAAAAACTAATTTACCTTCTTTCTTTAGTAAATGAAATTTCTTCTTGTGTAAAGGTTTCAAGATTATATTACCACATGGACTCTTATTTTATGCAGTATTTCCCAACTTTCTCTGCTATATTTCCTTGTGTTTTTATGTATTTCTGTGTACAATGTATAGGTGAATTTCAGGTTGGACTCATAACATTTAATATGATGCTTCATGTCAATGAATCCTCCGTGAAGCTTAACATCACTGAACTTGCTGAATTTATTGCCAATGAGCTTGAAGTTAATATCTCACAGGTACTTTAAAGTTCAAATTTTCACCATTCATTTTCACTCCTGTGATGACCTCCAGTTAGTATGGGATTGTGAGTAGATCGTTTCTAGATCTCTTTTTTATATTATCTTTTAAAAGGCACCCATTAATGGTGTGNGAGGGTAGCTACTGCTATACAAGTTTTGCTTACACAACTGCCAATTGTCTGATCTTTAGGTTCACGTGCTGAACTTTACATCAGAGGGAACTGATTTCTTCATTCGATGGGCCATTTTCCCTGCCGATTCTGCTGATTTTATTTCCAACTCCACAGCAATGGTAAAAGTTCTTAGTCTTACAGCGCCTAGGCTATATATATAAGTAAAATAATTGGAGTATCTTCAACCAACTTTTTGTGACTGTAAATGGTGATTCATAGGGCATAATTTCTCGCTTAAAGGAACATCACATGCAGCTTCCTGAAAAGTTCGGAAGTTATCAGCTGGCTGAATTGAATGTTGAACCTCCGTTAAAGCAGTGAGATATCTTCCTGAATGCTTTACTTCTTACTGCATTTAGAGCACATGGGATTGCACTACCCTACCATATTGCTTGTTTTTGTTTTTGTCGATTCTACCTTAACTGGATCAGAGGCATTTTTAGTTACTAGTTAAAAAGTTTTTCTGATGAAAGAGCAAGGATTTATATGCATGCTACTTACATTTACTGACCAATTTTCGGCAAAGTACCTTCAAAGCTTAGCATTGACTCCTGAATCGCAGTAACTCCTAATATGTGTACCTGTGTTGTGCCTACCTATATAGTGATTTTCCTTTTGCGCAGAAGGTTAGGTAGCTTTAAAATTACTATTGAGCAGTTGTATTGAGATCTTAGTTGTTTGCATGTATATGTTGTGTCTTGTTTACATTCTTATTCGCAACTTGTTTTTGCTACCACTTGGACTAATTTTCCCTCCCATATCGCTGGATCATGTAACTCTTTTCTCTCCGTGTCTGGATTTATACAGGACGTGGATGAAACAGCACATCTGGTCTATAATGACTATTGGATTAGCAGTTACCTTAGTTGTTGGACTGGCTGGCGCAAGTACATGGTTGATTTGGAGATACAGACGGAGGGAGCTGGGTTCTTATGAGCCTGTTGGCACAATTAGTCCCGAGCAAGAGCTTCAGCCACTT

mRNA sequence

AGCGGGGAGGGCATTTTACCGGGTCCACCCTGCGTCAATCTCCGCCCTCTCTCTTCCGTCTGCTCCGTCGCGTGCGGCCACCGGCGCACCATGTCTCCGTCTGGCGCCAATTCACCTTCTGCTATTGTCATCTATTGCCTAGTCGGTTTCAATCTGTTGCGCACGGTTTTCTCCTCATCTGTGGACTCAAGAGATTTCGATTATCAGCAGCGCCCCGTGATTCTTCCTCTCTACATCTCGACTACGAATTCCACTCATCACCGCGTCTTGAACCGTGATCATCGTTTCCGGCACCTGCAGAAATTGGACCAGCCCCACTCCTCAACTGCTCGAATGAGGCTGCACGATGACCTCCTTACCAACGGGTTCGTCTTTCAGAGGTTCTGGGACGTCGATATTTATAGGTATTACACGACGCGTCTATGGATTGGGACTCCTCCGCAAGAATTCGCTCTTATTGTGGACACCGGAAGTACAGTGACCTACGTTCCATGCTCTAACTGCGAAGAGTGCGGGAACCATCAGGATCCAAAGTTCCAACCAGAGTTGTCAAGCACATATCAACCTGTGAAGTGCAATATCAATTGTAACTGTGATGACAAGGGAGTCCAGTGTACATATGAGAGACGGTATGCAGAAATGAGCACTAGCAGTGGTATGCTTGCTGAGGACATCATGTCATTTGGAAAGGAAAGTGAACTTGTACCCCAGCGTGCTGTATTTGGTTGTGAAACTATGGAAACTGGTGACCTTTATACCCAACGTGCTGATGGGATTATGGGTTTGGGCCGTGGTTCACTCAGTGTGATGGACCAGCTTGCCAACAAGGGTGTTGTGAGCGATTCATTTTCGTTATGTTACGGTGGGATGGATGTTGGTGGGGGTGCAATGGTTCTTGGTGGGATCTCTTCACCCCCTGGTATGGTATTTACCCACTCGGACCCATCTCGAAGCCCATATTATAATATTGAGTTGAAGGAAATACATGTTGCTGGGAAGCCATTGAAGTTGAATCCGAACACTTTTGATGGGAAGTATGGTACTGTCTTGGATAGTGGAACTACATATGCTTATTTTCCTGAAAAAGCCTACTATGCATTTAAAGATGCTATAATGAAGAAAATTAATTTCCTGAAGCAAATTAGTGGTCCTGACCCGAATTTTAAGGATATTTGTTTTTCTGGTGCTGGAAGGGATGTCAATGAACTTCCAAAAGTTTTTCCAGAGGTTGATTTTGTTTTTGCGAATGGTCAAAAAATTTCACTGTCTCCGGAGAATTACTTGTTCCGGCATACTAAGGTTAGTGGTGCCTATTGTCTGGGAATCTTTAAAAATGGGAATGATCAGACTACACTTTTGGGAGGAATTGTTGTTCGTAATACTCTTGTCACTTATGATCGGAAAAACACCTCAATCGGGTTTTGGAAGACTAATTGTTCTGAACTTTGGAAGAATCTGCATTATCTCGCTCCACCTCCAGCACCTTTACCTTCCTATGGTCAGAATACAAGTAAAGAAACTTCCCCACTTGGTTCTCCAACTGGGCTATTTCTTTCTGGTGAATTTCAGGTTGGACTCATAACATTTAATATGATGCTTCATGTCAATGAATCCTCCGTGAAGCTTAACATCACTGAACTTGCTGAATTTATTGCCAATGAGCTTGAAGTTAATATCTCACAGGTTCACGTGCTGAACTTTACATCAGAGGGAACTGATTTCTTCATTCGATGGGCCATTTTCCCTGCCGATTCTGCTGATTTTATTTCCAACTCCACAGCAATGGGCATAATTTCTCGCTTAAAGGAACATCACATGCAGCTTCCTGAAAAGTTCGGAAGTTATCAGCTGGCTGAATTGAATGTTGAACCTCCGTTAAAGCAGACGTGGATGAAACAGCACATCTGGTCTATAATGACTATTGGATTAGCAGTTACCTTAGTTGTTGGACTGGCTGGCGCAAGTACATGGTTGATTTGGAGATACAGACGGAGGGAGCTGGGTTCTTATGAGCCTGTTGGCACAATTAGTCCCGAGCAAGAGCTTCAGCCACTT

Coding sequence (CDS)

AGCGGGGAGGGCATTTTACCGGGTCCACCCTGCGTCAATCTCCGCCCTCTCTCTTCCGTCTGCTCCGTCGCGTGCGGCCACCGGCGCACCATGTCTCCGTCTGGCGCCAATTCACCTTCTGCTATTGTCATCTATTGCCTAGTCGGTTTCAATCTGTTGCGCACGGTTTTCTCCTCATCTGTGGACTCAAGAGATTTCGATTATCAGCAGCGCCCCGTGATTCTTCCTCTCTACATCTCGACTACGAATTCCACTCATCACCGCGTCTTGAACCGTGATCATCGTTTCCGGCACCTGCAGAAATTGGACCAGCCCCACTCCTCAACTGCTCGAATGAGGCTGCACGATGACCTCCTTACCAACGGGTTCGTCTTTCAGAGGTTCTGGGACGTCGATATTTATAGGTATTACACGACGCGTCTATGGATTGGGACTCCTCCGCAAGAATTCGCTCTTATTGTGGACACCGGAAGTACAGTGACCTACGTTCCATGCTCTAACTGCGAAGAGTGCGGGAACCATCAGGATCCAAAGTTCCAACCAGAGTTGTCAAGCACATATCAACCTGTGAAGTGCAATATCAATTGTAACTGTGATGACAAGGGAGTCCAGTGTACATATGAGAGACGGTATGCAGAAATGAGCACTAGCAGTGGTATGCTTGCTGAGGACATCATGTCATTTGGAAAGGAAAGTGAACTTGTACCCCAGCGTGCTGTATTTGGTTGTGAAACTATGGAAACTGGTGACCTTTATACCCAACGTGCTGATGGGATTATGGGTTTGGGCCGTGGTTCACTCAGTGTGATGGACCAGCTTGCCAACAAGGGTGTTGTGAGCGATTCATTTTCGTTATGTTACGGTGGGATGGATGTTGGTGGGGGTGCAATGGTTCTTGGTGGGATCTCTTCACCCCCTGGTATGGTATTTACCCACTCGGACCCATCTCGAAGCCCATATTATAATATTGAGTTGAAGGAAATACATGTTGCTGGGAAGCCATTGAAGTTGAATCCGAACACTTTTGATGGGAAGTATGGTACTGTCTTGGATAGTGGAACTACATATGCTTATTTTCCTGAAAAAGCCTACTATGCATTTAAAGATGCTATAATGAAGAAAATTAATTTCCTGAAGCAAATTAGTGGTCCTGACCCGAATTTTAAGGATATTTGTTTTTCTGGTGCTGGAAGGGATGTCAATGAACTTCCAAAAGTTTTTCCAGAGGTTGATTTTGTTTTTGCGAATGGTCAAAAAATTTCACTGTCTCCGGAGAATTACTTGTTCCGGCATACTAAGGTTAGTGGTGCCTATTGTCTGGGAATCTTTAAAAATGGGAATGATCAGACTACACTTTTGGGAGGAATTGTTGTTCGTAATACTCTTGTCACTTATGATCGGAAAAACACCTCAATCGGGTTTTGGAAGACTAATTGTTCTGAACTTTGGAAGAATCTGCATTATCTCGCTCCACCTCCAGCACCTTTACCTTCCTATGGTCAGAATACAAGTAAAGAAACTTCCCCACTTGGTTCTCCAACTGGGCTATTTCTTTCTGGTGAATTTCAGGTTGGACTCATAACATTTAATATGATGCTTCATGTCAATGAATCCTCCGTGAAGCTTAACATCACTGAACTTGCTGAATTTATTGCCAATGAGCTTGAAGTTAATATCTCACAGGTTCACGTGCTGAACTTTACATCAGAGGGAACTGATTTCTTCATTCGATGGGCCATTTTCCCTGCCGATTCTGCTGATTTTATTTCCAACTCCACAGCAATGGGCATAATTTCTCGCTTAAAGGAACATCACATGCAGCTTCCTGAAAAGTTCGGAAGTTATCAGCTGGCTGAATTGAATGTTGAACCTCCGTTAAAGCAGACGTGGATGAAACAGCACATCTGGTCTATAATGACTATTGGATTAGCAGTTACCTTAGTTGTTGGACTGGCTGGCGCAAGTACATGGTTGATTTGGAGATACAGACGGAGGGAGCTGGGTTCTTATGAGCCTGTTGGCACAATTAGTCCCGAGCAAGAGCTTCAGCCACTT

Protein sequence

SGEGILPGPPCVNLRPLSSVCSVACGHRRTMSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYLAPPPAPLPSYGQNTSKETSPLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFTSEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQTWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL
Homology
BLAST of MS008717 vs. NCBI nr
Match: XP_022139880.1 (aspartic proteinase-like protein 2 [Momordica charantia])

HSP 1 Score: 1313.1 bits (3397), Expect = 0.0e+00
Identity = 641/655 (97.86%), Postives = 641/655 (97.86%), Query Frame = 0

Query: 31  MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL 90
           MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL
Sbjct: 1   MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL 60

Query: 91  NRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTPPQEF 150
           NRDHRFRHLQ LDQPHSSTARMRLHDDLLTNG             YYTTRLWIGTPPQEF
Sbjct: 61  NRDHRFRHLQXLDQPHSSTARMRLHDDLLTNG-------------YYTTRLWIGTPPQEF 120

Query: 151 ALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERR 210
           ALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERR
Sbjct: 121 ALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERR 180

Query: 211 YAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVM 270
           YAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVM
Sbjct: 181 YAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVM 240

Query: 271 DQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHV 330
           DQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHV
Sbjct: 241 DQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHV 300

Query: 331 AGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKD 390
           AGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKD
Sbjct: 301 AGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKD 360

Query: 391 ICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT 450
           ICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT
Sbjct: 361 ICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT 420

Query: 451 TLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYLAPPPAPLPSYGQNTSKETSPL 510
           TLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYLAPPPAPLPSYGQNTSKETSPL
Sbjct: 421 TLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYLAPPPAPLPSYGQNTSKETSPL 480

Query: 511 GSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFTSE 570
           GSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFTSE
Sbjct: 481 GSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFTSE 540

Query: 571 GTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQTW 630
           GTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQTW
Sbjct: 541 GTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQTW 600

Query: 631 MKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL 686
           MKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL
Sbjct: 601 MKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL 642

BLAST of MS008717 vs. NCBI nr
Match: XP_038898702.1 (aspartic proteinase 39-like [Benincasa hispida])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 575/682 (84.31%), Postives = 608/682 (89.15%), Query Frame = 0

Query: 15  RPLSSVCSVACG-----HRRTMSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQ 74
           R LSSV SV        HR TMSP  A+S SAIV YCLVGFNLL  + SSSV +RDFDYQ
Sbjct: 20  RVLSSVISVPSAPSRTVHRHTMSPPWADSYSAIVFYCLVGFNLLGRILSSSVHARDFDYQ 79

Query: 75  QRPVILPLYISTTNSTHHRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFW 134
           Q+PVILPLYIS  NSTH RVL+RDHR RHLQ L +PHSS ARMRLHDDLLTNG       
Sbjct: 80  QQPVILPLYISPMNSTHQRVLDRDHRLRHLQNLGKPHSSNARMRLHDDLLTNG------- 139

Query: 135 DVDIYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQP 194
                 YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNC ECGNHQDP+FQPELSSTYQP
Sbjct: 140 ------YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCVECGNHQDPRFQPELSSTYQP 199

Query: 195 VKCNINCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETG 254
           VKCN++CNCDD GVQCTYERRYAEMSTSSG+LAEDIMSFGKESELVPQRAVFGCETMETG
Sbjct: 200 VKCNVDCNCDDNGVQCTYERRYAEMSTSSGVLAEDIMSFGKESELVPQRAVFGCETMETG 259

Query: 255 DLYTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMV 314
           DLYTQRADGIMGLGRG+LSVMDQL  K V+S+SFSLCYGGMDVGGGAMVLGGISSPPGMV
Sbjct: 260 DLYTQRADGIMGLGRGTLSVMDQLVEKRVISNSFSLCYGGMDVGGGAMVLGGISSPPGMV 319

Query: 315 FTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKD 374
           FTHSDPSRSPYYNIELKEIHVAGKPLKLNP+TFDGKYG +LDSGTTYAYFPEKAYYAFKD
Sbjct: 320 FTHSDPSRSPYYNIELKEIHVAGKPLKLNPSTFDGKYGAILDSGTTYAYFPEKAYYAFKD 379

Query: 375 AIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLF 434
           AIMKKI+FLKQI GPDPNFKDICFSGAGRDV EL KVFPEVD VFANGQKISLSPENYLF
Sbjct: 380 AIMKKISFLKQIGGPDPNFKDICFSGAGRDVTELSKVFPEVDMVFANGQKISLSPENYLF 439

Query: 435 RHTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYL 494
           RHTKVSGAYCLG+FKNGNDQTTLLGGI+VRNTLVTYDR+NT+IGFWKTNCSELWKNLHYL
Sbjct: 440 RHTKVSGAYCLGVFKNGNDQTTLLGGIIVRNTLVTYDRENTTIGFWKTNCSELWKNLHYL 499

Query: 495 ------APPPAPLPSYGQNTSKETSPLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLN 554
                 APPPAPLPSYGQNTSKE  P GSP+  FLSGEFQVG+ITFNMMLHVN+SSVKLN
Sbjct: 500 SPAPPPAPPPAPLPSYGQNTSKEVPPPGSPSVPFLSGEFQVGVITFNMMLHVNKSSVKLN 559

Query: 555 ITELAEFIANELEVNISQVHVLNFTSEGTDFFIRWAIFPADSADFISNSTAMGIISRLKE 614
           ITELAEFIANELEVN+SQVHVLNFT   T+FFIRWAIFPAD A +ISN+TAM IISRLKE
Sbjct: 560 ITELAEFIANELEVNVSQVHVLNFTLGETEFFIRWAIFPADPAGYISNTTAMDIISRLKE 619

Query: 615 HHMQLPEKFGSYQLAELNVEPPLKQTWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYR 674
           H +QLPEKFGSYQL ELNVEPPLK+TWM+QH WS+MTIG+AVTLVVGLA  STWLIWRYR
Sbjct: 620 HDLQLPEKFGSYQLVELNVEPPLKKTWMEQHFWSMMTIGVAVTLVVGLAAVSTWLIWRYR 679

Query: 675 RRELGSYEPVGTISPEQELQPL 686
           RREL SYEPVG + PEQELQPL
Sbjct: 680 RRELSSYEPVGVVGPEQELQPL 688

BLAST of MS008717 vs. NCBI nr
Match: XP_008452619.1 (PREDICTED: aspartic proteinase-like protein 2 [Cucumis melo])

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 562/657 (85.54%), Postives = 599/657 (91.17%), Query Frame = 0

Query: 31  MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL 90
           MSP+  NS SAIV  CLVGFNLL  + SSSVDSRD DYQQRPV+LPLYIS TNSTH RV 
Sbjct: 1   MSPTCDNSYSAIVFCCLVGFNLLGMILSSSVDSRDLDYQQRPVLLPLYISPTNSTHRRVF 60

Query: 91  NRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTPPQEF 150
           +RDHR RHLQ L +PHSS ARMRLHDDLLTNG             YYTTRLWIGTPPQEF
Sbjct: 61  DRDHRLRHLQNLVKPHSSNARMRLHDDLLTNG-------------YYTTRLWIGTPPQEF 120

Query: 151 ALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERR 210
           ALIVDTGSTVTYVPCSNC ECGNHQDP+FQPELSSTYQPVKCN++CNCD+ GVQCTYERR
Sbjct: 121 ALIVDTGSTVTYVPCSNCVECGNHQDPRFQPELSSTYQPVKCNVDCNCDENGVQCTYERR 180

Query: 211 YAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVM 270
           YAEMSTSSG+LAED+MSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRG+LSVM
Sbjct: 181 YAEMSTSSGVLAEDVMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGTLSVM 240

Query: 271 DQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHV 330
           DQL  KGVVS+SFSLCYGGMDVGGGAMVLGGISSPPGMVF+HSDPSRSPYYNIELKEIHV
Sbjct: 241 DQLVGKGVVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFSHSDPSRSPYYNIELKEIHV 300

Query: 331 AGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKD 390
           AGKPLKLNP TFDGKYG +LDSGTTYAYFPEKAYYAFKDAIMKKI+FLKQI+GPDPNFKD
Sbjct: 301 AGKPLKLNPRTFDGKYGAILDSGTTYAYFPEKAYYAFKDAIMKKISFLKQINGPDPNFKD 360

Query: 391 ICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT 450
           ICFSGAGRDV ELPKVFPEVD VFA+GQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT
Sbjct: 361 ICFSGAGRDVTELPKVFPEVDMVFADGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT 420

Query: 451 TLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYL--APPPAPLPSYGQNTSKETS 510
           TLLGGI+VRNTLVTY+R+N++IGFWKTNCSELWKNLHYL  APPPAPLPS+  NTSKE  
Sbjct: 421 TLLGGIIVRNTLVTYNRENSTIGFWKTNCSELWKNLHYLSPAPPPAPLPSHVLNTSKEVP 480

Query: 511 PLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFT 570
           P GSP+  FLSGEFQVG+ITFNMMLHVN+SSVKLNITELAEFIANELEV++SQVHVLNFT
Sbjct: 481 PPGSPSVPFLSGEFQVGVITFNMMLHVNKSSVKLNITELAEFIANELEVSVSQVHVLNFT 540

Query: 571 SEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQ 630
           S  TDFFIRWAIFPADSA +ISNSTAM IISRLKEH +QLPEKFGSYQL ELNVEPPLK+
Sbjct: 541 SGETDFFIRWAIFPADSAGYISNSTAMDIISRLKEHDLQLPEKFGSYQLVELNVEPPLKK 600

Query: 631 TWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL 686
           TWM+QH WSIMTIGLAVTLVVGLA  STWLIWRYRRR++ SYEPVG + PEQELQP+
Sbjct: 601 TWMEQHFWSIMTIGLAVTLVVGLAAGSTWLIWRYRRRDMSSYEPVGVVGPEQELQPI 644

BLAST of MS008717 vs. NCBI nr
Match: KAG6591408.1 (Aspartic proteinase-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 561/661 (84.87%), Postives = 598/661 (90.47%), Query Frame = 0

Query: 27  HRRTMSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTH 86
           H+ TMSP   +  SAI +YCLVGFNLL  +FSSSVDSRDFDYQQRPVILPLYIS TNSTH
Sbjct: 81  HQHTMSPPSTSLHSAIFVYCLVGFNLLGAIFSSSVDSRDFDYQQRPVILPLYISPTNSTH 140

Query: 87  HRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTP 146
            RVL+RDHR RHL  L++PHSS ARMRL+DDLLTNG             YYTTRLWIGTP
Sbjct: 141 QRVLDRDHRLRHLLNLEKPHSSNARMRLYDDLLTNG-------------YYTTRLWIGTP 200

Query: 147 PQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCT 206
           PQEFALIVDTGSTVTYVPCSNC +CGNHQDP+FQP+LSS+YQPVKC+I+C+CDD GVQCT
Sbjct: 201 PQEFALIVDTGSTVTYVPCSNCVDCGNHQDPRFQPKLSSSYQPVKCHIDCSCDDNGVQCT 260

Query: 207 YERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGS 266
           YERRYAEMSTSSG+LAEDIMSFGKESEL+PQRAVFGCETMETGDLYTQRADGIMGLGRG+
Sbjct: 261 YERRYAEMSTSSGVLAEDIMSFGKESELLPQRAVFGCETMETGDLYTQRADGIMGLGRGT 320

Query: 267 LSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELK 326
           LSVMDQL  KG VS+SFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSD SRSPYYNIELK
Sbjct: 321 LSVMDQLVGKGAVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDTSRSPYYNIELK 380

Query: 327 EIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDP 386
           EIHVAGKPLKLNP TFDGKYG+VLDSGTTYAYFPEKAYYAFKDA+MKKI+FLKQI+GPDP
Sbjct: 381 EIHVAGKPLKLNPRTFDGKYGSVLDSGTTYAYFPEKAYYAFKDALMKKISFLKQINGPDP 440

Query: 387 NFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG 446
           NFKDICFSGAGRDV+EL KVFPEV  VFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG
Sbjct: 441 NFKDICFSGAGRDVSELSKVFPEVAMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG 500

Query: 447 NDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYL--APPPAPLPSYGQNTS 506
           NDQTTLLGGI+VRNTLVTYDR+NT IGFWKTNCSELWKNLHYL  APPPAPLPSYGQNTS
Sbjct: 501 NDQTTLLGGIIVRNTLVTYDRENTKIGFWKTNCSELWKNLHYLSPAPPPAPLPSYGQNTS 560

Query: 507 KETSPLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHV 566
            E  P  SPT  FLSGEFQVG+ITFNM+LH N+SSVKLNITELAEFIANELEVN+SQVHV
Sbjct: 561 NEIPPPVSPTVPFLSGEFQVGVITFNMVLHANKSSVKLNITELAEFIANELEVNVSQVHV 620

Query: 567 LNFTSEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEP 626
           LNFTS  TDFFIRWAIFPADSA +ISNSTAM IISRLKEH +QLPEKFGSYQL ELNVEP
Sbjct: 621 LNFTSGETDFFIRWAIFPADSAGYISNSTAMDIISRLKEHDLQLPEKFGSYQLVELNVEP 680

Query: 627 PLKQTWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQP 686
           PLK+TWM+QH WS+M+I  AVTLVVGLA  STWLIWRYRRREL SYEPVG + PEQELQP
Sbjct: 681 PLKKTWMEQHFWSMMSIVAAVTLVVGLAAGSTWLIWRYRRRELSSYEPVGAVGPEQELQP 728

BLAST of MS008717 vs. NCBI nr
Match: XP_022976987.1 (aspartic proteinase-like protein 2 [Cucurbita maxima])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 559/661 (84.57%), Postives = 597/661 (90.32%), Query Frame = 0

Query: 27  HRRTMSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTH 86
           H+ TMSP   +  SAI +YCLVGFN L  +FSSSVDSRDFDYQQRPVILPLYIS TNSTH
Sbjct: 59  HQHTMSPPSTSLHSAIFVYCLVGFNFLGAIFSSSVDSRDFDYQQRPVILPLYISPTNSTH 118

Query: 87  HRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTP 146
            RVL+RDHR RHL  L++PHSS ARMRL+DDLLTNG             YYTTRLWIGTP
Sbjct: 119 QRVLDRDHRLRHLLNLEKPHSSNARMRLYDDLLTNG-------------YYTTRLWIGTP 178

Query: 147 PQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCT 206
           PQEFALIVDTGSTVTYVPCSNC +CGNHQDP+FQP+LSS+YQPVKCNI+C+CDD GVQCT
Sbjct: 179 PQEFALIVDTGSTVTYVPCSNCVDCGNHQDPRFQPKLSSSYQPVKCNIDCSCDDNGVQCT 238

Query: 207 YERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGS 266
           YERRYAEMSTSSG+LAEDIMSFGKESEL+PQRAVFGCETMETGDLYTQRADGIMGLGRG+
Sbjct: 239 YERRYAEMSTSSGVLAEDIMSFGKESELLPQRAVFGCETMETGDLYTQRADGIMGLGRGT 298

Query: 267 LSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELK 326
           LSVMDQL  KG VS+SFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSD SRSPYYNIELK
Sbjct: 299 LSVMDQLVGKGAVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDTSRSPYYNIELK 358

Query: 327 EIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDP 386
           EIHVAGKPLKLNP TFDGKYG+VLDSGTTYAYFPEKAYYAFKDA+MKKI+FLKQI+GPDP
Sbjct: 359 EIHVAGKPLKLNPKTFDGKYGSVLDSGTTYAYFPEKAYYAFKDALMKKISFLKQINGPDP 418

Query: 387 NFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG 446
           NFKDICFSGAGRDV+EL KVFPEV  VFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG
Sbjct: 419 NFKDICFSGAGRDVSELSKVFPEVAMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG 478

Query: 447 NDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYL--APPPAPLPSYGQNTS 506
           NDQTTLLGGI+VRNTLVTYDR+NT IGFWKTNCSELWKNLHYL  APPPAPLPSYGQNTS
Sbjct: 479 NDQTTLLGGIIVRNTLVTYDRENTKIGFWKTNCSELWKNLHYLSPAPPPAPLPSYGQNTS 538

Query: 507 KETSPLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHV 566
            E  P  SPT  FLSGEFQVG+ITFNM+LH N+SSVKLNITELAEFIANELEVN+SQVH+
Sbjct: 539 NEIPPPVSPTVPFLSGEFQVGVITFNMVLHANKSSVKLNITELAEFIANELEVNVSQVHM 598

Query: 567 LNFTSEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEP 626
           LNFTS GTDFFIRWAIFPADSA +ISNSTAM IISRLK+H +QLPEKFGSYQL ELNVEP
Sbjct: 599 LNFTSGGTDFFIRWAIFPADSAGYISNSTAMDIISRLKKHDLQLPEKFGSYQLVELNVEP 658

Query: 627 PLKQTWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQP 686
           PLK+TWM+ H WS+M+I  AVTLVVGLA  STWLIWRYRRREL SYEPVG + PEQELQP
Sbjct: 659 PLKKTWMELHFWSMMSIVAAVTLVVGLAAGSTWLIWRYRRRELSSYEPVGAVGPEQELQP 706

BLAST of MS008717 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 8.2e-38
Identity = 133/424 (31.37%), Postives = 199/424 (46.93%), Query Frame = 0

Query: 89  VLNRDHRF----RHLQKLDQPHS-STARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWI 148
           V N  H+F    + L +L    S   ARM  + DL   G         D    Y T++ +
Sbjct: 30  VFNVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPLGGDS-----RADSIGLYFTKIKL 89

Query: 149 GTPPQEFALIVDTGSTVTYVPCSNCEECGNHQD-----PKFQPELSSTYQPVKCNIN-CN 208
           G+PP+E+ + VDTGS + +V C+ C +C    D       +  + SST + V C  + C+
Sbjct: 90  GSPPKEYYVQVDTGSDILWVNCAPCPKCPVKTDLGIPLSLYDSKTSSTSKNVGCEDDFCS 149

Query: 209 -------CDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGK-----ESELVPQRAVFGCET 268
                  C  K   C+Y   Y + STS G   +D ++  +      +  + Q  VFGC  
Sbjct: 150 FIMQSETCGAK-KPCSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEVVFGCGK 209

Query: 269 METGDL--YTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGIS 328
            ++G L       DGIMG G+ + S++ QLA  G     FS C   M+ GGG   +G + 
Sbjct: 210 NQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMN-GGGIFAVGEVE 269

Query: 329 SPPGMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPN--TFDGKYGTVLDSGTTYAYFPE 388
           SP  +V T        +YN+ LK + V G P+ L P+  + +G  GT++DSGTT AY P+
Sbjct: 270 SP--VVKTTPIVPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTIIDSGTTLAYLPQ 329

Query: 389 KAYYAFKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFVFANGQKIS 448
             Y    +++++KI   +Q+          CFS      +   K FP V+  F +  K+S
Sbjct: 330 NLY----NSLIEKITAKQQVKLHMVQETFACFS----FTSNTDKAFPVVNLHFEDSLKLS 389

Query: 449 LSPENYLFRHTKVSGAYCLGIFKNG-----NDQTTLLGGIVVRNTLVTYDRKNTSIGFWK 481
           + P +YLF  +     YC G    G          LLG +V+ N LV YD +N  IG+  
Sbjct: 390 VYPHDYLF--SLREDMYCFGWQSGGMTTQDGADVILLGDLVLSNKLVVYDLENEVIGWAD 434

BLAST of MS008717 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 3.1e-37
Identity = 117/374 (31.28%), Postives = 178/374 (47.59%), Query Frame = 0

Query: 131 VDIYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPEL-----SS 190
           VD    Y T++ +G+PP+E+ + VDTGS + ++ C  C +C    +  F+  L     SS
Sbjct: 68  VDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKTNLNFRLSLFDMNASS 127

Query: 191 TYQPVKCN-------INCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGK-----ESE 250
           T + V C+          +     + C+Y   YA+ STS G    D+++  +     ++ 
Sbjct: 128 TSKKVGCDDDFCSFISQSDSCQPALGCSYHIVYADESTSDGKFIRDMLTLEQVTGDLKTG 187

Query: 251 LVPQRAVFGCETMETGDLYT--QRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMD 310
            + Q  VFGC + ++G L       DG+MG G+ + SV+ QLA  G     FS C   + 
Sbjct: 188 PLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCLDNVK 247

Query: 311 VGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFDGKYGTVLD 370
            GGG   +G + SP   V T        +YN+ L  + V G  L L P +     GT++D
Sbjct: 248 -GGGIFAVGVVDSP--KVKTTPMVPNQMHYNVMLMGMDVDGTSLDL-PRSIVRNGGTIVD 307

Query: 371 SGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVD 430
           SGTT AYFP+  Y +  + I+ +      I   +  F+  CFS +      + + FP V 
Sbjct: 308 SGTTLAYFPKVLYDSLIETILARQPVKLHI--VEETFQ--CFSFS----TNVDEAFPPVS 367

Query: 431 FVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG-----NDQTTLLGGIVVRNTLVTYD 481
           F F +  K+++ P +YLF  T     YC G    G       +  LLG +V+ N LV YD
Sbjct: 368 FEFEDSVKLTVYPHDYLF--TLEEELYCFGWQAGGLTTDERSEVILLGDLVLSNKLVVYD 427

BLAST of MS008717 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 3.0e-32
Identity = 128/469 (27.29%), Postives = 199/469 (42.43%), Query Frame = 0

Query: 58  SSSVDSRDF---DYQQRPVILPLYISTTNSTHH----------RVLNRDHRFRHLQKLDQ 117
           SSS+   DF   D  Q P+ +   +   N+TH           R+L+RD RF  +   + 
Sbjct: 19  SSSISFPDFQIIDVLQPPLTVTATLPDFNNTHFSDESSSKYTLRLLHRD-RFPSVTYRNH 78

Query: 118 PHSSTARMRLHDDLLT------NGFVF---QRFWDVDIY------------RYYTTRLWI 177
            H   ARMR   D ++      +G V       ++V+ +              Y  R+ +
Sbjct: 79  HHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRIGV 138

Query: 178 GTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNI-------NC 237
           G+PP++  +++D+GS + +V C  C+ C    DP F P  S +Y  V C         N 
Sbjct: 139 GSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCGSSVCDRIENS 198

Query: 238 NCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRA 297
            C   G  C YE  Y + S + G LA + ++F K    V +    GC     G      A
Sbjct: 199 GCHSGG--CRYEVMYGDGSYTKGTLALETLTFAK---TVVRNVAMGCGHRNRGMFI--GA 258

Query: 298 DGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTH--SD 357
            G++G+G GS+S + QL+ +   +  + L   G D   G++V G  + P G  +     +
Sbjct: 259 AGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTD-STGSLVFGREALPVGASWVPLVRN 318

Query: 358 PSRSPYYNIELKEIHVAGKPLKLNPNTFD----GKYGTVLDSGTTYAYFPEKAYYAFKDA 417
           P    +Y + LK + V G  + L    FD    G  G V+D+GT     P  AY AF+D 
Sbjct: 319 PRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDG 378

Query: 418 IMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFR 477
              +   L + SG   +  D C+  +G     +    P V F F  G  ++L   N+L  
Sbjct: 379 FKSQTANLPRASG--VSIFDTCYDLSG----FVSVRVPTVSFYFTEGPVLTLPARNFLM- 438

Query: 478 HTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNC 480
               SG YC   F       +++G I      V++D  N  +GF    C
Sbjct: 439 PVDDSGTYCFA-FAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of MS008717 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.5e-31
Identity = 106/359 (29.53%), Postives = 165/359 (45.96%), Query Frame = 0

Query: 137 YTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNI-N 196
           Y +R+ +GTP +E  L++DTGS V ++ C  C +C    DP F P  SSTY+ + C+   
Sbjct: 162 YFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCSAPQ 221

Query: 197 CNCDD----KGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDL 256
           C+  +    +  +C Y+  Y + S + G LA D ++FG   ++       GC     G L
Sbjct: 222 CSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKI--NNVALGCGHDNEG-L 281

Query: 257 YTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMV------LGGISSP 316
           +T  A G++GLG G LS+ +Q+      + SFS C    D G  + +      LGG  + 
Sbjct: 282 FTGAA-GLLGLGGGVLSITNQMK-----ATSFSYCLVDRDSGKSSSLDFNSVQLGGGDAT 341

Query: 317 PGMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFD----GKYGTVLDSGTTYAYFPE 376
             ++    +     +Y + L    V G+ + L    FD    G  G +LD GT       
Sbjct: 342 APLL---RNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVTRLQT 401

Query: 377 KAYYAFKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKV-FPEVDFVFANGQKI 436
           +AY + +DA +K    LK+ S     F D C+     D + L  V  P V F F  G+ +
Sbjct: 402 QAYNSLRDAFLKLTVNLKKGSSSISLF-DTCY-----DFSSLSTVKVPTVAFHFTGGKSL 461

Query: 437 SLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNC 480
            L  +NYL      SG +C   F   +   +++G +  + T +TYD     IG     C
Sbjct: 462 DLPAKNYLI-PVDDSGTFCFA-FAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of MS008717 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.5e-28
Identity = 105/351 (29.91%), Postives = 153/351 (43.59%), Query Frame = 0

Query: 137 YTTRLWIGTPPQEFALIVDTGSTVTYVPCSNC-EECGNHQDPKFQPELSSTYQPVKCNIN 196
           Y   + IGTP  + +L+ DTGS +T+  C  C   C + ++PKF P  SSTYQ V C+ +
Sbjct: 132 YIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCS-S 191

Query: 197 CNCDD----KGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDL 256
             C+D        C Y   Y + S + G LA++   F   +  V +   FGC     G L
Sbjct: 192 PMCEDAESCSASNCVYSIVYGDKSFTQGFLAKE--KFTLTNSDVLEDVYFGCGENNQG-L 251

Query: 257 YTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGG-GAMVLGGISSPPGMVF 316
           +   A G++GLG G LS+  Q       ++ FS C         G +  G       + F
Sbjct: 252 FDGVA-GLLGLGPGKLSLPAQTTT--TYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKF 311

Query: 317 THSDPSRSPY-YNIELKEIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKD 376
           T      S + Y I++  I V  K L + PN+F  + G ++DSGT +   P K Y   + 
Sbjct: 312 TPISSFPSAFNYGIDIIGISVGDKELAITPNSFSTE-GAIIDSGTVFTRLPTKVYAELRS 371

Query: 377 AIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLF 436
              +K++  K  SG      D C+   G D       +P + F FA    + L     + 
Sbjct: 372 VFKEKMSSYKSTSG--YGLFDTCYDFTGLDT----VTYPTIAFSFAGSTVVELDGSG-IS 431

Query: 437 RHTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTL-VTYDRKNTSIGFWKTNC 480
              K+S   CL     GND    + G V + TL V YD     +GF    C
Sbjct: 432 LPIKIS-QVCLAF--AGNDDLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of MS008717 vs. ExPASy TrEMBL
Match: A0A6J1CDI9 (aspartic proteinase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111010681 PE=3 SV=1)

HSP 1 Score: 1313.1 bits (3397), Expect = 0.0e+00
Identity = 641/655 (97.86%), Postives = 641/655 (97.86%), Query Frame = 0

Query: 31  MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL 90
           MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL
Sbjct: 1   MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL 60

Query: 91  NRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTPPQEF 150
           NRDHRFRHLQ LDQPHSSTARMRLHDDLLTNG             YYTTRLWIGTPPQEF
Sbjct: 61  NRDHRFRHLQXLDQPHSSTARMRLHDDLLTNG-------------YYTTRLWIGTPPQEF 120

Query: 151 ALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERR 210
           ALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERR
Sbjct: 121 ALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERR 180

Query: 211 YAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVM 270
           YAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVM
Sbjct: 181 YAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVM 240

Query: 271 DQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHV 330
           DQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHV
Sbjct: 241 DQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHV 300

Query: 331 AGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKD 390
           AGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKD
Sbjct: 301 AGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKD 360

Query: 391 ICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT 450
           ICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT
Sbjct: 361 ICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT 420

Query: 451 TLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYLAPPPAPLPSYGQNTSKETSPL 510
           TLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYLAPPPAPLPSYGQNTSKETSPL
Sbjct: 421 TLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYLAPPPAPLPSYGQNTSKETSPL 480

Query: 511 GSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFTSE 570
           GSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFTSE
Sbjct: 481 GSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFTSE 540

Query: 571 GTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQTW 630
           GTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQTW
Sbjct: 541 GTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQTW 600

Query: 631 MKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL 686
           MKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL
Sbjct: 601 MKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL 642

BLAST of MS008717 vs. ExPASy TrEMBL
Match: A0A1S3BTN6 (aspartic proteinase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103493586 PE=3 SV=1)

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 562/657 (85.54%), Postives = 599/657 (91.17%), Query Frame = 0

Query: 31  MSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTHHRVL 90
           MSP+  NS SAIV  CLVGFNLL  + SSSVDSRD DYQQRPV+LPLYIS TNSTH RV 
Sbjct: 1   MSPTCDNSYSAIVFCCLVGFNLLGMILSSSVDSRDLDYQQRPVLLPLYISPTNSTHRRVF 60

Query: 91  NRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTPPQEF 150
           +RDHR RHLQ L +PHSS ARMRLHDDLLTNG             YYTTRLWIGTPPQEF
Sbjct: 61  DRDHRLRHLQNLVKPHSSNARMRLHDDLLTNG-------------YYTTRLWIGTPPQEF 120

Query: 151 ALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCTYERR 210
           ALIVDTGSTVTYVPCSNC ECGNHQDP+FQPELSSTYQPVKCN++CNCD+ GVQCTYERR
Sbjct: 121 ALIVDTGSTVTYVPCSNCVECGNHQDPRFQPELSSTYQPVKCNVDCNCDENGVQCTYERR 180

Query: 211 YAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGSLSVM 270
           YAEMSTSSG+LAED+MSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRG+LSVM
Sbjct: 181 YAEMSTSSGVLAEDVMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGTLSVM 240

Query: 271 DQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHV 330
           DQL  KGVVS+SFSLCYGGMDVGGGAMVLGGISSPPGMVF+HSDPSRSPYYNIELKEIHV
Sbjct: 241 DQLVGKGVVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFSHSDPSRSPYYNIELKEIHV 300

Query: 331 AGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKD 390
           AGKPLKLNP TFDGKYG +LDSGTTYAYFPEKAYYAFKDAIMKKI+FLKQI+GPDPNFKD
Sbjct: 301 AGKPLKLNPRTFDGKYGAILDSGTTYAYFPEKAYYAFKDAIMKKISFLKQINGPDPNFKD 360

Query: 391 ICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT 450
           ICFSGAGRDV ELPKVFPEVD VFA+GQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT
Sbjct: 361 ICFSGAGRDVTELPKVFPEVDMVFADGQKISLSPENYLFRHTKVSGAYCLGIFKNGNDQT 420

Query: 451 TLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYL--APPPAPLPSYGQNTSKETS 510
           TLLGGI+VRNTLVTY+R+N++IGFWKTNCSELWKNLHYL  APPPAPLPS+  NTSKE  
Sbjct: 421 TLLGGIIVRNTLVTYNRENSTIGFWKTNCSELWKNLHYLSPAPPPAPLPSHVLNTSKEVP 480

Query: 511 PLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHVLNFT 570
           P GSP+  FLSGEFQVG+ITFNMMLHVN+SSVKLNITELAEFIANELEV++SQVHVLNFT
Sbjct: 481 PPGSPSVPFLSGEFQVGVITFNMMLHVNKSSVKLNITELAEFIANELEVSVSQVHVLNFT 540

Query: 571 SEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEPPLKQ 630
           S  TDFFIRWAIFPADSA +ISNSTAM IISRLKEH +QLPEKFGSYQL ELNVEPPLK+
Sbjct: 541 SGETDFFIRWAIFPADSAGYISNSTAMDIISRLKEHDLQLPEKFGSYQLVELNVEPPLKK 600

Query: 631 TWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQPL 686
           TWM+QH WSIMTIGLAVTLVVGLA  STWLIWRYRRR++ SYEPVG + PEQELQP+
Sbjct: 601 TWMEQHFWSIMTIGLAVTLVVGLAAGSTWLIWRYRRRDMSSYEPVGVVGPEQELQPI 644

BLAST of MS008717 vs. ExPASy TrEMBL
Match: A0A6J1IH74 (aspartic proteinase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111477188 PE=3 SV=1)

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 559/661 (84.57%), Postives = 597/661 (90.32%), Query Frame = 0

Query: 27  HRRTMSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTH 86
           H+ TMSP   +  SAI +YCLVGFN L  +FSSSVDSRDFDYQQRPVILPLYIS TNSTH
Sbjct: 59  HQHTMSPPSTSLHSAIFVYCLVGFNFLGAIFSSSVDSRDFDYQQRPVILPLYISPTNSTH 118

Query: 87  HRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTP 146
            RVL+RDHR RHL  L++PHSS ARMRL+DDLLTNG             YYTTRLWIGTP
Sbjct: 119 QRVLDRDHRLRHLLNLEKPHSSNARMRLYDDLLTNG-------------YYTTRLWIGTP 178

Query: 147 PQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCT 206
           PQEFALIVDTGSTVTYVPCSNC +CGNHQDP+FQP+LSS+YQPVKCNI+C+CDD GVQCT
Sbjct: 179 PQEFALIVDTGSTVTYVPCSNCVDCGNHQDPRFQPKLSSSYQPVKCNIDCSCDDNGVQCT 238

Query: 207 YERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGS 266
           YERRYAEMSTSSG+LAEDIMSFGKESEL+PQRAVFGCETMETGDLYTQRADGIMGLGRG+
Sbjct: 239 YERRYAEMSTSSGVLAEDIMSFGKESELLPQRAVFGCETMETGDLYTQRADGIMGLGRGT 298

Query: 267 LSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELK 326
           LSVMDQL  KG VS+SFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSD SRSPYYNIELK
Sbjct: 299 LSVMDQLVGKGAVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDTSRSPYYNIELK 358

Query: 327 EIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDP 386
           EIHVAGKPLKLNP TFDGKYG+VLDSGTTYAYFPEKAYYAFKDA+MKKI+FLKQI+GPDP
Sbjct: 359 EIHVAGKPLKLNPKTFDGKYGSVLDSGTTYAYFPEKAYYAFKDALMKKISFLKQINGPDP 418

Query: 387 NFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG 446
           NFKDICFSGAGRDV+EL KVFPEV  VFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG
Sbjct: 419 NFKDICFSGAGRDVSELSKVFPEVAMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG 478

Query: 447 NDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYL--APPPAPLPSYGQNTS 506
           NDQTTLLGGI+VRNTLVTYDR+NT IGFWKTNCSELWKNLHYL  APPPAPLPSYGQNTS
Sbjct: 479 NDQTTLLGGIIVRNTLVTYDRENTKIGFWKTNCSELWKNLHYLSPAPPPAPLPSYGQNTS 538

Query: 507 KETSPLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNISQVHV 566
            E  P  SPT  FLSGEFQVG+ITFNM+LH N+SSVKLNITELAEFIANELEVN+SQVH+
Sbjct: 539 NEIPPPVSPTVPFLSGEFQVGVITFNMVLHANKSSVKLNITELAEFIANELEVNVSQVHM 598

Query: 567 LNFTSEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAELNVEP 626
           LNFTS GTDFFIRWAIFPADSA +ISNSTAM IISRLK+H +QLPEKFGSYQL ELNVEP
Sbjct: 599 LNFTSGGTDFFIRWAIFPADSAGYISNSTAMDIISRLKKHDLQLPEKFGSYQLVELNVEP 658

Query: 627 PLKQTWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQELQP 686
           PLK+TWM+ H WS+M+I  AVTLVVGLA  STWLIWRYRRREL SYEPVG + PEQELQP
Sbjct: 659 PLKKTWMELHFWSMMSIVAAVTLVVGLAAGSTWLIWRYRRRELSSYEPVGAVGPEQELQP 706

BLAST of MS008717 vs. ExPASy TrEMBL
Match: A0A6J1F7I1 (aspartic proteinase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111442809 PE=3 SV=1)

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 561/665 (84.36%), Postives = 597/665 (89.77%), Query Frame = 0

Query: 27  HRRTMSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTH 86
           H+ TMSP   +  SA+ +YCLVGFNLL  +FSSSVDSRDF YQQRPVILPLYIS TNSTH
Sbjct: 59  HQHTMSPPSTSLHSAVFVYCLVGFNLLGAIFSSSVDSRDFYYQQRPVILPLYISPTNSTH 118

Query: 87  HRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTP 146
            RVL+RDHR RHL  L++PHSS ARMRL+DDLLTNG             YYTTRLWIGTP
Sbjct: 119 QRVLDRDHRLRHLLNLEKPHSSNARMRLYDDLLTNG-------------YYTTRLWIGTP 178

Query: 147 PQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCT 206
           PQEFALIVDTGSTVTYVPCSNC +CGNHQDP+FQP+LSS+YQPVKCNI+C+CDD GVQCT
Sbjct: 179 PQEFALIVDTGSTVTYVPCSNCVDCGNHQDPRFQPKLSSSYQPVKCNIDCSCDDNGVQCT 238

Query: 207 YERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGS 266
           YERRYAEMSTSSG+LAEDIMSFGKESEL+PQRAVFGCETMETGDLYTQRADGIMGLGRG+
Sbjct: 239 YERRYAEMSTSSGVLAEDIMSFGKESELLPQRAVFGCETMETGDLYTQRADGIMGLGRGT 298

Query: 267 LSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELK 326
           LSVMDQL  KG VS+SFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSD SRSPYYNIELK
Sbjct: 299 LSVMDQLVGKGAVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDTSRSPYYNIELK 358

Query: 327 EIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDP 386
           EIHVAGKPLKLNP TFDGKYG+VLDSGTTYAYFPEKAYYAFKDA+MKKI+FLKQISGPDP
Sbjct: 359 EIHVAGKPLKLNPRTFDGKYGSVLDSGTTYAYFPEKAYYAFKDALMKKISFLKQISGPDP 418

Query: 387 NFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG 446
           NFKDICFSGAGRDV+EL KVFPEV  VFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG
Sbjct: 419 NFKDICFSGAGRDVSELSKVFPEVAMVFANGQKISLSPENYLFRHTKVSGAYCLGIFKNG 478

Query: 447 NDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYL------APPPAPLPSYG 506
           NDQTTLLGGI+VRNTLVTYDR+NT IGFWKTNCSELWKNLHYL      APPPAPLPSYG
Sbjct: 479 NDQTTLLGGIIVRNTLVTYDRENTKIGFWKTNCSELWKNLHYLSPAPPPAPPPAPLPSYG 538

Query: 507 QNTSKETSPLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITELAEFIANELEVNIS 566
           QNTS E  P  SPT  FLSGEFQVG+ITFNM+LH N+SSVKLNITELAEFIANELEVN+S
Sbjct: 539 QNTSNEIPPPVSPTVPFLSGEFQVGVITFNMVLHANKSSVKLNITELAEFIANELEVNVS 598

Query: 567 QVHVLNFTSEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQLPEKFGSYQLAEL 626
           QVHVLNFTS  TDFFIRWAIFPADSA +ISNSTAM IISRLKEH +QLPEKFGSYQL EL
Sbjct: 599 QVHVLNFTSGETDFFIRWAIFPADSAGYISNSTAMDIISRLKEHDLQLPEKFGSYQLVEL 658

Query: 627 NVEPPLKQTWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRRELGSYEPVGTISPEQ 686
           NVEPPLK+TWM+QH WS+M+I  AVTLVVGLA  STWLIWRYRRREL SYEPVG + PEQ
Sbjct: 659 NVEPPLKKTWMEQHFWSMMSIVAAVTLVVGLAAGSTWLIWRYRRRELSSYEPVGAVGPEQ 710

BLAST of MS008717 vs. ExPASy TrEMBL
Match: A0A5A7VA17 (Aspartic proteinase-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G001390 PE=3 SV=1)

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 565/689 (82.00%), Postives = 602/689 (87.37%), Query Frame = 0

Query: 27   HRRTMSPSGANSPSAIVIYCLVGFNLLRTVFSSSVDSRDFDYQQRPVILPLYISTTNSTH 86
            HR TMSP+  NS SAIV  CLVGFNLL  + SSSVDSRD DYQQRPV+LPLYIS TNSTH
Sbjct: 503  HRHTMSPTCDNSYSAIVFCCLVGFNLLGMILSSSVDSRDLDYQQRPVLLPLYISPTNSTH 562

Query: 87   HRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFWDVDIYRYYTTRLWIGTP 146
             RV +RDHR RHLQ L +PHSS ARMRLHDDLLTNG             YYTTRLWIGTP
Sbjct: 563  RRVFDRDHRLRHLQNLVKPHSSNARMRLHDDLLTNG-------------YYTTRLWIGTP 622

Query: 147  PQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQPVKCNINCNCDDKGVQCT 206
            PQEFALIVDTGSTVTYVPCSNC ECGNHQDP+FQPELSSTYQPVKCN++CNCD+ GVQCT
Sbjct: 623  PQEFALIVDTGSTVTYVPCSNCVECGNHQDPRFQPELSSTYQPVKCNVDCNCDENGVQCT 682

Query: 207  YERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGS 266
            YERRYAEMSTSSG+LAED+MSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRG+
Sbjct: 683  YERRYAEMSTSSGVLAEDVMSFGKESELVPQRAVFGCETMETGDLYTQRADGIMGLGRGT 742

Query: 267  LSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELK 326
            LSVMDQL  KGVVS+SFSLCYGGMDVGGGAMVLGGISSPPGMVF+HSDPSRSPYYNIELK
Sbjct: 743  LSVMDQLVGKGVVSNSFSLCYGGMDVGGGAMVLGGISSPPGMVFSHSDPSRSPYYNIELK 802

Query: 327  EIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDP 386
            EIHVAGKPLKLNP TFDGKYG +LDSGTTYAYFPEKAYYAFKDAIMKKI+FLKQI+GPDP
Sbjct: 803  EIHVAGKPLKLNPRTFDGKYGAILDSGTTYAYFPEKAYYAFKDAIMKKISFLKQINGPDP 862

Query: 387  NFKDICFSGAG----------------------------RDVNELPKVFPEVDFVFANGQ 446
            NFKDICFSGAG                            RDV ELPKVFPEVD VFA+GQ
Sbjct: 863  NFKDICFSGAGRYFYFLLGASYFRFILSGLIVEHLFFCARDVTELPKVFPEVDMVFADGQ 922

Query: 447  KISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTN 506
            KISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGI+VRNTLVTY+R+N++IGFWKTN
Sbjct: 923  KISLSPENYLFRHTKVSGAYCLGIFKNGNDQTTLLGGIIVRNTLVTYNRENSTIGFWKTN 982

Query: 507  CSELWKNLHYL--APPPAPLPSYGQNTSKETSPLGSPTGLFLSGEFQVGLITFNMMLHVN 566
            CSELWKNLHYL  APPPAPLPS+  NTSKE  P GSP+  FLSGEFQVG+ITFNMMLHVN
Sbjct: 983  CSELWKNLHYLSPAPPPAPLPSHVLNTSKEVPPPGSPSVPFLSGEFQVGVITFNMMLHVN 1042

Query: 567  ESSVKLNITELAEFIANELEVNISQVHVLNFTSEGTDFFIRWAIFPADSADFISNSTAMG 626
            +SSVKLNITELAEFIANELEV++SQVHVLNFTS  TDFFIRWAIFPADSA +ISNSTAM 
Sbjct: 1043 KSSVKLNITELAEFIANELEVSVSQVHVLNFTSGETDFFIRWAIFPADSAGYISNSTAMD 1102

Query: 627  IISRLKEHHMQLPEKFGSYQLAELNVEPPLKQTWMKQHIWSIMTIGLAVTLVVGLAGAST 686
            IISRLKEH +QLPEKFGSYQL ELNVEPPLK+TWM+QH WSIMTIGLAVTLVVGLA  ST
Sbjct: 1103 IISRLKEHDLQLPEKFGSYQLVELNVEPPLKKTWMEQHFWSIMTIGLAVTLVVGLAAGST 1162

BLAST of MS008717 vs. TAIR 10
Match: AT5G43100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 735.7 bits (1898), Expect = 3.3e-212
Identity = 369/621 (59.42%), Postives = 462/621 (74.40%), Query Frame = 0

Query: 70  QRPVILPLYISTTNSTHHRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFW 129
           + P+I PL  S   S   R    D R R L +   P+   A M+L+DDLL+NG       
Sbjct: 28  ESPMIFPLSYS---SLPPRPRVEDFRRRRLHQSQLPN---AHMKLYDDLLSNG------- 87

Query: 130 DVDIYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQP 189
                 YYTTRLWIGTPPQEFALIVDTGSTVTYVPCS C++CG HQDPKFQPELS++YQ 
Sbjct: 88  ------YYTTRLWIGTPPQEFALIVDTGSTVTYVPCSTCKQCGKHQDPKFQPELSTSYQA 147

Query: 190 VKCNINCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETG 249
           +KCN +CNCDD+G  C YERRYAEMS+SSG+L+ED++SFG ES+L PQRAVFGCE  ETG
Sbjct: 148 LKCNPDCNCDDEGKLCVYERRYAEMSSSSGVLSEDLISFGNESQLSPQRAVFGCENEETG 207

Query: 250 DLYTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMV 309
           DL++QRADGIMGLGRG LSV+DQL +KGV+ D FSLCYGGM+VGGGAMVLG IS PPGMV
Sbjct: 208 DLFSQRADGIMGLGRGKLSVVDQLVDKGVIEDVFSLCYGGMEVGGGAMVLGKISPPPGMV 267

Query: 310 FTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKD 369
           F+HSDP RSPYYNI+LK++HVAGK LKLNP  F+GK+GTVLDSGTTYAYFP++A+ A KD
Sbjct: 268 FSHSDPFRSPYYNIDLKQMHVAGKSLKLNPKVFNGKHGTVLDSGTTYAYFPKEAFIAIKD 327

Query: 370 AIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFVFANGQKISLSPENYLF 429
           A++K+I  LK+I GPDPN+ D+CFSGAGRDV E+   FPE+   F NGQK+ LSPENYLF
Sbjct: 328 AVIKEIPSLKRIHGPDPNYDDVCFSGAGRDVAEIHNFFPEIAMEFGNGQKLILSPENYLF 387

Query: 430 RHTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHYL 489
           RHTKV GAYCLGIF +  D TTLLGGIVVRNTLVTYDR+N  +GF KTNCS++W+ L   
Sbjct: 388 RHTKVRGAYCLGIFPD-RDSTTLLGGIVVRNTLVTYDRENDKLGFLKTNCSDIWRRL--A 447

Query: 490 AP-PPAPLPSYGQNTSKETSP----LGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNI 549
           AP  PAP     QN S   SP      SPT   L G F+VG+ITF + + VN SS+K   
Sbjct: 448 APESPAPTSPISQNKSSNISPSPATSESPTS-HLPGVFRVGVITFEVSISVNNSSLKPKF 507

Query: 550 TELAEFIANELEVNISQVHVLNFTSEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEH 609
           +E+A+FIA+EL++  +QV +LNF+S G ++ ++W +FP  S+++ISN+TA+ I+  LKE+
Sbjct: 508 SEIADFIAHELDIQSAQVRLLNFSSSGNEYRLKWGVFPPQSSEYISNTTALNIMLLLKEN 567

Query: 610 HMQLPEKFGSYQLAELNVEPPLKQTWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRR 669
            ++LP +FGSY+L E   E   KQ+W ++H+  ++  G  ++L+V        L+WR R+
Sbjct: 568 RLRLPGQFGSYKLLEWKAEQKKKQSWWEKHLLGVVG-GAMISLLVTSVMIKLALVWRRRK 624

Query: 670 RELGSYEPVGTISPEQELQPL 686
           +E  +YEPV     EQELQPL
Sbjct: 628 QEEATYEPVNAAIKEQELQPL 624

BLAST of MS008717 vs. TAIR 10
Match: AT3G50050.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 689.1 bits (1777), Expect = 3.6e-198
Identity = 347/619 (56.06%), Postives = 441/619 (71.24%), Query Frame = 0

Query: 70  QRPVILPLYISTTNSTHHRVLNRDHRFRHLQKLDQPHSSTARMRLHDDLLTNGFVFQRFW 129
           +RP++ PL++S  NS+  R ++  H  R L K D      +RMRL+DDLL NG       
Sbjct: 42  RRPMVFPLFLSQPNSS-SRSISIPH--RKLHKSDSKSLPHSRMRLYDDLLING------- 101

Query: 130 DVDIYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPKFQPELSSTYQP 189
                 YYTTRLWIGTPPQ FALIVD+GSTVTYVPCS+CE+CG HQDPKFQPE+SSTYQP
Sbjct: 102 ------YYTTRLWIGTPPQMFALIVDSGSTVTYVPCSDCEQCGKHQDPKFQPEMSSTYQP 161

Query: 190 VKCNINCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGKESELVPQRAVFGCETMETG 249
           VKCN++CNCDD   QC YER YAE S+S G+L ED++SFG ES+L PQRAVFGCET+ETG
Sbjct: 162 VKCNMDCNCDDDREQCVYEREYAEHSSSKGVLGEDLISFGNESQLTPQRAVFGCETVETG 221

Query: 250 DLYTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGGAMVLGGISSPPGMV 309
           DLY+QRADGI+GLG+G LS++DQL +KG++S+SF LCYGGMDVGGG+M+LGG   P  MV
Sbjct: 222 DLYSQRADGIIGLGQGDLSLVDQLVDKGLISNSFGLCYGGMDVGGGSMILGGFDYPSDMV 281

Query: 310 FTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFDGKYGTVLDSGTTYAYFPEKAYYAFKD 369
           FT SDP RSPYYNI+L  I VAGK L L+   FDG++G VLDSGTTYAY P+ A+ AF++
Sbjct: 282 FTDSDPDRSPYYNIDLTGIRVAGKQLSLHSRVFDGEHGAVLDSGTTYAYLPDAAFAAFEE 341

Query: 370 AIMKKINFLKQISGPDPNFKDICFS-GAGRDVNELPKVFPEVDFVFANGQKISLSPENYL 429
           A+M++++ LKQI GPDPNFKD CF   A   V+EL K+FP V+ VF +GQ   LSPENY+
Sbjct: 342 AVMREVSTLKQIDGPDPNFKDTCFQVAASNYVSELSKIFPSVEMVFKSGQSWLLSPENYM 401

Query: 430 FRHTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNTSIGFWKTNCSELWKNLHY 489
           FRH+KV GAYCLG+F NG D TTLLGGIVVRNTLV YDR+N+ +GFW+TNCSEL   LH 
Sbjct: 402 FRHSKVHGAYCLGVFPNGKDHTTLLGGIVVRNTLVVYDRENSKVGFWRTNCSELSDRLHI 461

Query: 490 -LAPPPAPLPSYGQNTSKETSPLGSPTGLFLSGEFQVGLITFNMMLHVNESSVKLNITEL 549
             APPPA LPS   N S  +S         LSG  QVG I  ++ L VN S +K  I +L
Sbjct: 462 DGAPPPATLPSNDSNPSHNSSS-------NLSGVTQVGQINLDIQLTVNSSYLKPRIEDL 521

Query: 550 AEFIANELEVNISQVHVLNFTSEGTDFFIRWAIFPADSADFISNSTAMGIISRLKEHHMQ 609
           ++  + EL+V  SQV + N TS+G +  +R  + P + + + SN TA  I+SR   H ++
Sbjct: 522 SKIFSKELDVKSSQVSLSNLTSKGNESLVRMVVLPPEPSTWFSNVTATNIVSRFTNHQIK 581

Query: 610 LPEKFGSYQLAELNVEPPLKQTWMKQHIWSIMTIGLAVTLVVGLAGASTWLIWRYRRREL 669
           LPE FG+YQL    +EPP K+T    +   ++ IG+ + ++VGL+    WLIW+ ++  +
Sbjct: 582 LPEIFGNYQLVNYKLEPPRKRT---NNNIVVIAIGI-IAVIVGLSAYGAWLIWKRKQTSI 632

Query: 670 GSYEPVG-TISPEQELQPL 686
             Y+PV   I  EQELQP+
Sbjct: 642 -PYKPVDEAIVAEQELQPI 632

BLAST of MS008717 vs. TAIR 10
Match: AT5G22850.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-51
Identity = 130/378 (34.39%), Postives = 192/378 (50.79%), Query Frame = 0

Query: 129 WDVDIYRYYTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPK-----FQPEL 188
           +D  +   Y T+L +GTPP++F + VDTGS V +V C++C  C      +     F P  
Sbjct: 73  FDPFVVGLYYTKLRLGTPPRDFYVQVDTGSDVLWVSCASCNGCPQTSGLQIQLNFFDPGS 132

Query: 189 SSTYQPVKC----------NINCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGK--E 248
           S T  P+ C          + +  C  +   C Y  +Y + S +SG    D++ F     
Sbjct: 133 SVTASPISCSDQRCSWGIQSSDSGCSVQNNLCAYTFQYGDGSGTSGFYVSDVLQFDMIVG 192

Query: 249 SELVPQR---AVFGCETMETGDLY--TQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLC 308
           S LVP      VFGC T +TGDL    +  DGI G G+  +SV+ QLA++G+    FS C
Sbjct: 193 SSLVPNSTAPVVFGCSTSQTGDLVKSDRAVDGIFGFGQQGMSVISQLASQGIAPRVFSHC 252

Query: 309 YGGMDVGGGAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTF--DG 368
             G + GGG +VLG I   P MVFT   PS+ P+YN+ L  I V G+ L +NP+ F    
Sbjct: 253 LKGENGGGGILVLGEIVE-PNMVFTPLVPSQ-PHYNVNLLSISVNGQALPINPSVFSTSN 312

Query: 369 KYGTVLDSGTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELP 428
             GT++D+GTT AY  E AY  F +AI    N + Q   P  +  + C+         + 
Sbjct: 313 GQGTIIDTGTTLAYLSEAAYVPFVEAI---TNAVSQSVRPVVSKGNQCYV----ITTSVG 372

Query: 429 KVFPEVDFVFANGQKISLSPENYLFRHTKVSG--AYCLGIFKNGNDQTTLLGGIVVRNTL 481
            +FP V   FA G  + L+P++YL +   V G   +C+G  +  N   T+LG +V+++ +
Sbjct: 373 DIFPPVSLNFAGGASMFLNPQDYLIQQNNVGGTAVWCIGFQRIQNQGITILGDLVLKDKI 432

BLAST of MS008717 vs. TAIR 10
Match: AT1G08210.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 196.1 bits (497), Expect = 9.5e-50
Identity = 131/368 (35.60%), Postives = 192/368 (52.17%), Query Frame = 0

Query: 137 YTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQDPK-----FQPELSSTYQPVK 196
           Y T++ +GTPP+EF + +DTGS V +V C++C  C    + +     F P +SS+   V 
Sbjct: 84  YYTKVKLGTPPREFNVQIDTGSDVLWVSCTSCNGCPKTSELQIQLSFFDPGVSSSASLVS 143

Query: 197 CN---------INCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGK--ESELVPQRA- 256
           C+             C    + C+Y  +Y + S +SG    D MSF     S L    + 
Sbjct: 144 CSDRRCYSNFQTESGCSPNNL-CSYSFKYGDGSGTSGYYISDFMSFDTVITSTLAINSSA 203

Query: 257 --VFGCETMETGDLYTQR--ADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGGG 316
             VFGC  +++GDL   R   DGI GLG+GSLSV+ QLA +G+    FS C  G   GGG
Sbjct: 204 PFVFGCSNLQSGDLQRPRRAVDGIFGLGQGSLSVISQLAVQGLAPRVFSHCLKGDKSGGG 263

Query: 317 AMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFD--GKYGTVLDSG 376
            MVLG I   P  V+T   PS+ P+YN+ L+ I V G+ L ++P+ F      GT++D+G
Sbjct: 264 IMVLGQIKR-PDTVYTPLVPSQ-PHYNVNLQSIAVNGQILPIDPSVFTIATGDGTIIDTG 323

Query: 377 TTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDFV 436
           TT AY P++AY  F  A+    N + Q   P       CF     DV+    VFP+V   
Sbjct: 324 TTLAYLPDEAYSPFIQAV---ANAVSQYGRPITYESYQCFEITAGDVD----VFPQVSLS 383

Query: 437 FANGQKISLSPENYL-FRHTKVSGAYCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNTSI 481
           FA G  + L P  YL    +  S  +C+G  +  + + T+LG +V+++ +V YD     I
Sbjct: 384 FAGGASMVLGPRAYLQIFSSSGSSIWCIGFQRMSHRRITILGDLVLKDKVVVYDLVRQRI 441

BLAST of MS008717 vs. TAIR 10
Match: AT2G36670.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 179.5 bits (454), Expect = 9.2e-45
Identity = 124/370 (33.51%), Postives = 187/370 (50.54%), Query Frame = 0

Query: 137 YTTRLWIGTPPQEFALIVDTGSTVTYVPCSNCEECGNHQD-----PKFQPELSSTYQPVK 196
           Y T++ +G+PP EF + +DTGS + +V CS+C  C +          F    S T   V 
Sbjct: 105 YFTKVKLGSPPTEFNVQIDTGSDILWVTCSSCSNCPHSSGLGIDLHFFDAPGSLTAGSVT 164

Query: 197 CN----------INCNCDDKGVQCTYERRYAEMSTSSGMLAEDIMSFGK---ESELVPQR 256
           C+              C +   QC Y  RY + S +SG    D   F     ES +    
Sbjct: 165 CSDPICSSVFQTTAAQCSENN-QCGYSFRYGDGSGTSGYYMTDTFYFDAILGESLVANSS 224

Query: 257 A--VFGCETMETGDL--YTQRADGIMGLGRGSLSVMDQLANKGVVSDSFSLCYGGMDVGG 316
           A  VFGC T ++GDL    +  DGI G G+G LSV+ QL+++G+    FS C  G   GG
Sbjct: 225 APIVFGCSTYQSGDLTKSDKAVDGIFGFGKGKLSVVSQLSSRGITPPVFSHCLKGDGSGG 284

Query: 317 GAMVLGGISSPPGMVFTHSDPSRSPYYNIELKEIHVAGKPLKLNPNTFDGK--YGTVLDS 376
           G  VLG I   PGMV++   PS+ P+YN+ L  I V G+ L L+   F+     GT++D+
Sbjct: 285 GVFVLGEI-LVPGMVYSPLVPSQ-PHYNLNLLSIGVNGQMLPLDAAVFEASNTRGTIVDT 344

Query: 377 GTTYAYFPEKAYYAFKDAIMKKINFLKQISGPDPNFKDICFSGAGRDVNELPKVFPEVDF 436
           GTT  Y  ++AY  F +AI    N + Q+  P  +  + C+  +      +  +FP V  
Sbjct: 345 GTTLTYLVKEAYDLFLNAIS---NSVSQLVTPIISNGEQCYLVS----TSISDMFPSVSL 404

Query: 437 VFANGQKISLSPENYLFRHTKVSGA--YCLGIFKNGNDQTTLLGGIVVRNTLVTYDRKNT 481
            FA G  + L P++YLF +    GA  +C+G F+   ++ T+LG +V+++ +  YD    
Sbjct: 405 NFAGGASMMLRPQDYLFHYGIYDGASMWCIG-FQKAPEEQTILGDLVLKDKVFVYDLARQ 463

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139880.10.0e+0097.86aspartic proteinase-like protein 2 [Momordica charantia][more]
XP_038898702.10.0e+0084.31aspartic proteinase 39-like [Benincasa hispida][more]
XP_008452619.10.0e+0085.54PREDICTED: aspartic proteinase-like protein 2 [Cucumis melo][more]
KAG6591408.10.0e+0084.87Aspartic proteinase-like protein 2, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022976987.10.0e+0084.57aspartic proteinase-like protein 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q4V3D28.2e-3831.37Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Q9S9K43.1e-3731.28Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q9LHE33.0e-3227.29Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LS401.5e-3129.53Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LEW31.5e-2829.91Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CDI90.0e+0097.86aspartic proteinase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC11101068... [more]
A0A1S3BTN60.0e+0085.54aspartic proteinase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103493586 PE=3 ... [more]
A0A6J1IH740.0e+0084.57aspartic proteinase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111477188 P... [more]
A0A6J1F7I10.0e+0084.36aspartic proteinase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111442809... [more]
A0A5A7VA170.0e+0082.00Aspartic proteinase-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT5G43100.13.3e-21259.42Eukaryotic aspartyl protease family protein [more]
AT3G50050.13.6e-19856.06Eukaryotic aspartyl protease family protein [more]
AT5G22850.11.3e-5134.39Eukaryotic aspartyl protease family protein [more]
AT1G08210.19.5e-5035.60Eukaryotic aspartyl protease family protein [more]
AT2G36670.19.2e-4533.51Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 143..163
score: 50.56
coord: 348..359
score: 32.64
coord: 451..466
score: 25.12
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 57..516
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 321..474
e-value: 2.7E-27
score: 95.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 130..300
e-value: 2.7E-48
score: 166.5
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 308..484
e-value: 3.9E-45
score: 155.8
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 134..484
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 137..301
e-value: 4.1E-38
score: 131.4
NoneNo IPR availablePANTHERPTHR13683:SF847EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 57..516
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 152..163
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 137..475
score: 46.60437
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 136..479
e-value: 1.08423E-77
score: 247.561

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008717.1MS008717.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity