Homology
BLAST of MS008715 vs. NCBI nr
Match:
XP_022159803.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Momordica charantia])
HSP 1 Score: 819.3 bits (2115), Expect = 1.4e-233
Identity = 390/392 (99.49%), Postives = 390/392 (99.49%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG
Sbjct: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF
Sbjct: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP
Sbjct: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
G RADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE
Sbjct: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
DYGFEEE ERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM
Sbjct: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP
Sbjct: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
BLAST of MS008715 vs. NCBI nr
Match:
XP_004152001.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothetical protein Csa_017526 [Cucumis sativus])
HSP 1 Score: 723.8 bits (1867), Expect = 8.1e-205
Identity = 338/394 (85.79%), Postives = 363/394 (92.13%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+TEIENCEAN
Sbjct: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KMLRNVLRSVIPNA NLRHI LQTG KHY+GSFESI KS+Q+HDPPFTEDLPRLE PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
YY ED+L+EE+E ++K+LTWAVIRPN IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG + WE F+VASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEDYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
IE+YGF+EEGE L+L MK+K VW+EIVKENQLQQTK+EE+GTWWF D S SG ID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
BLAST of MS008715 vs. NCBI nr
Match:
XP_022940360.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita moschata] >KAG6608438.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037773.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 713.8 bits (1841), Expect = 8.3e-202
Identity = 336/394 (85.28%), Postives = 358/394 (90.86%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRPNWNADHPIEYIQCD+SDP+DAEAKLS+L DVTHVFYVTWTN TEIENCE NG
Sbjct: 61 VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KMLRNVLRSVIPNA NLRHI LQTG KHYIGSFE+I TQ+HDPPFTED+PRL+SPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
YY ED+LFEE+E ++K+LTWAVIRPNTIFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
Query: 241 FPGTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPGT++ WEG+ V SDADLIAEQHIWTAVDPYAKNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEDYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
IEDYGF EEGERL L + MK K +VWEEIVKENQLQ TK+E+V WW D LS SG ID
Sbjct: 301 IEDYGF-EEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
BLAST of MS008715 vs. NCBI nr
Match:
XP_008447374.1 (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo] >KAA0037954.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo var. makuwa])
HSP 1 Score: 711.4 bits (1835), Expect = 4.1e-201
Identity = 332/394 (84.26%), Postives = 360/394 (91.37%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+ EI+NCEAN
Sbjct: 61 VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
+MLRNVLRSVIPNA NLRHI LQTG KHY+GSFES+ KS+Q+H+PPFTEDLPRLE PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180
Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
YY ED+LFEE+E ++K+LTWAVIRPN IFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPGT+ WE F+ ASDADL+AEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEDYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
IE+YGF EEGERL+L MK+K VW+EIVKENQLQ TK+EE+G WW+ D S SG ID
Sbjct: 301 IEEYGF-EEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of MS008715 vs. NCBI nr
Match:
XP_023523285.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 709.5 bits (1830), Expect = 1.6e-200
Identity = 334/394 (84.77%), Postives = 356/394 (90.36%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRPNWNADHPIEYIQCD+SD +DAEAKLS+L DVTHVFYVTWTN TEIENCE NG
Sbjct: 61 VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KMLRNVLRSVIPNA NLRHI LQTG KHYIGSFE+I TQ+HDPPFTED+PRL++PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
YY ED+LFEE+E ++K+LTWAVIRPNTIFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
Query: 241 FPGTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPGT++ WEG+ V SDADLIAEQHIWTAVDPYAKNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEDYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
IEDYGF EEGERL L + MK K VWEEIVKENQLQ TK+E+V WW D LS SG ID
Sbjct: 301 IEDYGF-EEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
BLAST of MS008715 vs. ExPASy Swiss-Prot
Match:
Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)
HSP 1 Score: 642.5 bits (1656), Expect = 3.1e-183
Identity = 295/392 (75.26%), Postives = 335/392 (85.46%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHPI+YIQCD+SD +D +KLS L DVTHVFYVTWTNR +E ENCEANG
Sbjct: 61 VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
MLRNVL+++IP A NLRH+ LQTG KHY+G F ++ G +HDPPFTED+PRL+ NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDG---PRHDPPFTEDMPRLQIQNF 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YY EDILFEE+++ + +TW++ RPN IFGFSP+SLMNIVGTLCVYAAICKHEG PL FP
Sbjct: 181 YYTQEDILFEEIKKIETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFP 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
G++ WEGF ASDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF IE
Sbjct: 241 GSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIE 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
+YGF EEG+ L L + MK K VWEE+VKENQLQ+ K+EEVG WWFAD L GMIDSM
Sbjct: 301 EYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDSM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKE+GFLGFRNS NSFISWIDK+KA+KIVP
Sbjct: 361 NKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388
BLAST of MS008715 vs. ExPASy Swiss-Prot
Match:
A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)
HSP 1 Score: 613.2 bits (1580), Expect = 2.0e-174
Identity = 280/392 (71.43%), Postives = 326/392 (83.16%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKK +EDE PR++ESVAL++GVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP+WN DHPI YI CD+ D D EAKLS L DVTH+FY TWT RSTE ENCEANG
Sbjct: 61 VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KML+NVL ++IPN NL+HI LQTG KHY+G+FE + K HDPP TEDLPRL+S N+
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFEDLKSKC---HDPPLTEDLPRLDSQNY 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YY EDILFEEV++++ LTW+V RP TIFGFSP+S+MN+VGTLCVYAAICKHEG L+FP
Sbjct: 181 YYTQEDILFEEVQKKEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFP 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
G + W+G+ SDADLIAE IW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF +E
Sbjct: 241 GCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVE 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
G EEG+++ L+ MK+KG VW++IV+EN L TK+E+VG WWF+D L +D+M
Sbjct: 301 -CGEYEEGQQVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDTM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 NKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388
BLAST of MS008715 vs. ExPASy Swiss-Prot
Match:
A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)
HSP 1 Score: 606.7 bits (1563), Expect = 1.9e-172
Identity = 279/392 (71.17%), Postives = 325/392 (82.91%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKK+ +EDE PR++ESVAL++GVTGIVGNSLAEILPL+DTP GPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKRIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP+WN DHPI YI CD+ D D EAKLS L DVTH+FY TWT RSTE ENCEANG
Sbjct: 61 VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KML+NVL ++IPN NL+HI LQTG KHY+G+FE K +HDPP TEDLPRL+S NF
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFEDWKIK---RHDPPLTEDLPRLDSQNF 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YY EDILFEEV+++++LTW+V RP TIFGFSP+S+MN+VGTLCVYAAICKHEG L+FP
Sbjct: 181 YYTQEDILFEEVQKKESLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFP 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
G + W+G+ SDADLIAE IW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF +E
Sbjct: 241 GCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVE 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
G EEG+ + L+ MK+KG VW++IV+EN L TK+E+VG WWF+D L +D+M
Sbjct: 301 -CGEYEEGKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDTM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 NKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388
BLAST of MS008715 vs. ExPASy Swiss-Prot
Match:
A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)
HSP 1 Score: 597.8 bits (1540), Expect = 8.8e-170
Identity = 278/392 (70.92%), Postives = 316/392 (80.61%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAA+KKFEED+ PR +SVAL+IGVTGIVGNSLAEILP++DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAARKKFEEDDAPRDCQSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHP+EYIQCDISD D KLS L DVTH+F+VTW NR TE E CE NG
Sbjct: 61 VARRPRPAWNADHPVEYIQCDISDASDTHTKLSPLTDVTHIFWVTWANRPTESECCELNG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
MLRNVL ++IP A NL HI LQTG KHYIG FE+ + H+PPFTED+PRL +PNF
Sbjct: 121 TMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAFG--KIKPHEPPFTEDMPRLNAPNF 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YY LED+L E E++ L W+V RP IFGFSPFS+MNI+GTLCVYAAICKHE PLKFP
Sbjct: 181 YYTLEDMLVEASEKKAGLNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFP 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
GT+A W + VASDADLIAE IW AVDPYAKNEAFNC+NGD+FKWKH WKVLAEQF +E
Sbjct: 241 GTKAAWNCYSVASDADLIAEHQIWAAVDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVE 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
F+E + SL + MK+KG VWEEIV+EN L TK+E V TWWFAD L G ++DSM
Sbjct: 301 YAEFDESEKPTSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILGGECLLDSM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKEHG+LGFRN+KNS IS IDK KA+KIVP
Sbjct: 361 NKSKEHGYLGFRNTKNSLISVIDKMKAHKIVP 390
BLAST of MS008715 vs. ExPASy Swiss-Prot
Match:
Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)
HSP 1 Score: 594.3 bits (1531), Expect = 9.7e-169
Identity = 279/392 (71.17%), Postives = 317/392 (80.87%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKK+ EED+ SVAL++GVTGI+GNSLAEILPLADTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARR RP W+ D+PI Y+QCDISDP D++AKLS L DVTHVFYVTW NRSTE ENCEAN
Sbjct: 61 VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KM RNVL +VIPN NL+HISLQTG KHY+G FES + HDPP+TEDLPRL+ NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESYG--KIESHDPPYTEDLPRLKYMNF 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YY LEDI+ EEVE+++ LTW+V RP IFGFSP+S+MN+VGTLCVYAAICKHEG+ L+F
Sbjct: 181 YYDLEDIMLEEVEKKEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLRFT 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
G +A W+G+ SDADLIAE HIW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF +
Sbjct: 241 GCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGV- 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
G EEG L L+ MK K VWEEIV+EN L TK+++VG WWF D L +DSM
Sbjct: 301 GCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLDSM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKEHGFLGFRNSKN+FISWIDK KAYKIVP
Sbjct: 361 NKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389
BLAST of MS008715 vs. ExPASy TrEMBL
Match:
A0A6J1DZW3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Momordica charantia OX=3673 GN=LOC111026111 PE=3 SV=1)
HSP 1 Score: 819.3 bits (2115), Expect = 6.8e-234
Identity = 390/392 (99.49%), Postives = 390/392 (99.49%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG
Sbjct: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF
Sbjct: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP
Sbjct: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
G RADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE
Sbjct: 241 GNRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
DYGFEEE ERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM
Sbjct: 301 DYGFEEEDERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP
Sbjct: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 392
BLAST of MS008715 vs. ExPASy TrEMBL
Match:
A0A0A0L8Y0 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 PE=3 SV=1)
HSP 1 Score: 723.8 bits (1867), Expect = 3.9e-205
Identity = 338/394 (85.79%), Postives = 363/394 (92.13%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+TEIENCEAN
Sbjct: 61 VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KMLRNVLRSVIPNA NLRHI LQTG KHY+GSFESI KS+Q+HDPPFTEDLPRLE PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
YY ED+L+EE+E ++K+LTWAVIRPN IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPG + WE F+VASDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEDYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
IE+YGF+EEGE L+L MK+K VW+EIVKENQLQQTK+EE+GTWWF D S SG ID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
BLAST of MS008715 vs. ExPASy TrEMBL
Match:
A0A6J1FQC1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucurbita moschata OX=3662 GN=LOC111445997 PE=3 SV=1)
HSP 1 Score: 713.8 bits (1841), Expect = 4.0e-202
Identity = 336/394 (85.28%), Postives = 358/394 (90.86%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRPNWNADHPIEYIQCD+SDP+DAEAKLS+L DVTHVFYVTWTN TEIENCE NG
Sbjct: 61 VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
KMLRNVLRSVIPNA NLRHI LQTG KHYIGSFE+I TQ+HDPPFTED+PRL+SPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180
Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
YY ED+LFEE+E ++K+LTWAVIRPNTIFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
Query: 241 FPGTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPGT++ WEG+ V SDADLIAEQHIWTAVDPYAKNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEDYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
IEDYGF EEGERL L + MK K +VWEEIVKENQLQ TK+E+V WW D LS SG ID
Sbjct: 301 IEDYGF-EEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
BLAST of MS008715 vs. ExPASy TrEMBL
Match:
A0A5A7T3B3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001350 PE=3 SV=1)
HSP 1 Score: 711.4 bits (1835), Expect = 2.0e-201
Identity = 332/394 (84.26%), Postives = 360/394 (91.37%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+ EI+NCEAN
Sbjct: 61 VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
+MLRNVLRSVIPNA NLRHI LQTG KHY+GSFES+ KS+Q+H+PPFTEDLPRLE PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180
Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
YY ED+LFEE+E ++K+LTWAVIRPN IFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPGT+ WE F+ ASDADL+AEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEDYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
IE+YGF EEGERL+L MK+K VW+EIVKENQLQ TK+EE+G WW+ D S SG ID
Sbjct: 301 IEEYGF-EEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of MS008715 vs. ExPASy TrEMBL
Match:
A0A1S3BHA7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=3 SV=1)
HSP 1 Score: 711.4 bits (1835), Expect = 2.0e-201
Identity = 332/394 (84.26%), Postives = 360/394 (91.37%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHPIEYIQCD+SDPQDAE KLSQLADVTH+FYVTWTNR+ EI+NCEAN
Sbjct: 61 VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
+MLRNVLRSVIPNA NLRHI LQTG KHY+GSFES+ KS+Q+H+PPFTEDLPRLE PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180
Query: 181 YYALEDILFEEVE--ERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
YY ED+LFEE+E ++K+LTWAVIRPN IFGFSPFSLMNIVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240
Query: 241 FPGTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
FPGT+ WE F+ ASDADL+AEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
Query: 301 IEDYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMID 360
IE+YGF EEGERL+L MK+K VW+EIVKENQLQ TK+EE+G WW+ D S SG ID
Sbjct: 301 IEEYGF-EEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360
Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
SMNKSKEHGFLGFRNSKNSFISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393
BLAST of MS008715 vs. TAIR 10
Match:
AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 642.5 bits (1656), Expect = 2.2e-184
Identity = 295/392 (75.26%), Postives = 335/392 (85.46%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHPI+YIQCD+SD +D +KLS L DVTHVFYVTWTNR +E ENCEANG
Sbjct: 61 VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
MLRNVL+++IP A NLRH+ LQTG KHY+G F ++ G +HDPPFTED+PRL+ NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDG---PRHDPPFTEDMPRLQIQNF 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YY EDILFEE+++ + +TW++ RPN IFGFSP+SLMNIVGTLCVYAAICKHEG PL FP
Sbjct: 181 YYTQEDILFEEIKKIETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFP 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
G++ WEGF ASDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF IE
Sbjct: 241 GSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIE 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
+YGF EEG+ L L + MK K VWEE+VKENQLQ+ K+EEVG WWFAD L GMIDSM
Sbjct: 301 EYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDSM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKE+GFLGFRNS NSFISWIDK+KA+KIVP
Sbjct: 361 NKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388
BLAST of MS008715 vs. TAIR 10
Match:
AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 636.0 bits (1639), Expect = 2.1e-182
Identity = 294/392 (75.00%), Postives = 334/392 (85.20%), Query Frame = 0
Query: 1 MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYG 60
MSWWWAGAIGAA KK +EDEP +SFESVAL+IGVTGIVGNSLAEILPL+DTPGGPWKVYG
Sbjct: 1 MSWWWAGAIGAA-KKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
Query: 61 VARRPRPNWNADHPIEYIQCDISDPQDAEAKLSQLADVTHVFYVTWTNRSTEIENCEANG 120
VARRPRP WNADHPI+YIQCD+SD +D +KLS L DVTHVFYVTWTNR +E ENCEANG
Sbjct: 61 VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120
Query: 121 KMLRNVLRSVIPNATNLRHISLQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLESPNF 180
MLRNVL+++IP A NLRH+ LQTG KHY+G F ++ G +HDPPFTED+PRL+ NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDG---PRHDPPFTEDMPRLQIQNF 180
Query: 181 YYALEDILFEEVEERKNLTWAVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFP 240
YY EDILFEE+++ + +TW++ RPN IFGFSP+SLMNIVGTLCVYAAICKHEG PL FP
Sbjct: 181 YYTQEDILFEEIKKIETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFP 240
Query: 241 GTRADWEGFRVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIE 300
G++ WEGF ASDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF IE
Sbjct: 241 GSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIE 300
Query: 301 DYGFEEEGERLSLEKTMKEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTLSGSGMIDSM 360
+YGF EEG+ L L + MK K VWEE+VKENQLQ+ K+EEVG WWFAD L GMIDSM
Sbjct: 301 EYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDSM 360
Query: 361 NKSKEHGFLGFRNSKNSFISWIDKFKAYKIVP 393
NKSKE+GFLGFRNS NSFISWIDK+KA+KIVP
Sbjct: 361 NKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387
BLAST of MS008715 vs. TAIR 10
Match:
AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )
HSP 1 Score: 235.7 bits (600), Expect = 6.2e-62
Identity = 138/376 (36.70%), Postives = 206/376 (54.79%), Query Frame = 0
Query: 26 ESVALVIGVTGIVGNSLAEILPLADTPGGPWKVYGVARRPRPNWNADHPIEYIQCDISDP 85
E+VAL+ GVTG+VG + + L L PG W++YGVAR P N + +I CD+ +
Sbjct: 16 ENVALIFGVTGLVGREIVKTL-LMSKPG--WRIYGVARNPEIN-SMTKMYNFISCDLLNA 75
Query: 86 QDAEAKLSQLAD-VTHVFYVTWTNR---STEIENCEANGKMLRNVLRSVIPNATNLRHIS 145
+ + +LS L D V+HVF+VTW+ T+ E C N ML N L +++PNA L+H S
Sbjct: 76 SETKQRLSPLQDIVSHVFWVTWSGEFPLDTD-ECCVQNKTMLMNALDAILPNAKRLKHFS 135
Query: 146 LQTGAKHYIGSFESISGKSTQQHDPPFTEDLPRLES-PNFYYALEDILFEEVEERKNLTW 205
LQTG KHY+ E + ++E+ PR S NFYY LED+L E++ R ++ W
Sbjct: 136 LQTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKI-TRSSVVW 195
Query: 206 AVIRPNTIFGFSPFSLMNIVGTLCVYAAICKHEGRPLKFPGTRADW-EGFRVASDADLIA 265
+V RP + G S +L N +G+LCVY A+CK+ P F GTR W E + SD++L+A
Sbjct: 196 SVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVA 255
Query: 266 EQHIWTAVDPYA--KNEAFNCNNGDVFKWKHFWKVLAEQFNIEDYGFEEEGERLSLEKTM 325
EQHI+ A K EAFN NG F WK W + ++ ++ E + M
Sbjct: 256 EQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGREM 315
Query: 326 KEKGSVWEEIVKENQLQQTKVEEVGTWWFADFTL-SGSGMIDSMNKSKEHGFLGFRNSKN 385
E+ VW+EIV + +L +T++E++ W+F D ++ K GF + +
Sbjct: 316 VERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLD 375
Query: 386 SFISWIDKFKAYKIVP 393
S + WID + K++P
Sbjct: 376 SVLYWIDVMRDEKLIP 385
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022159803.1 | 1.4e-233 | 99.49 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Momordica charantia] | [more] |
XP_004152001.1 | 8.1e-205 | 85.79 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus] >KGN58303.1 hypothet... | [more] |
XP_022940360.1 | 8.3e-202 | 85.28 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita moschata] >KAG6608438.1 3-o... | [more] |
XP_008447374.1 | 4.1e-201 | 84.26 | PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo] >KAA0037954.... | [more] |
XP_023523285.1 | 1.6e-200 | 84.77 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
Q9STX2 | 3.1e-183 | 75.26 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... | [more] |
A0A221J5W8 | 2.0e-174 | 71.43 | (S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ... | [more] |
A0A221J5X3 | 1.9e-172 | 71.17 | (S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1... | [more] |
A0A1C9CX66 | 8.8e-170 | 70.92 | (S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... | [more] |
Q6PQJ9 | 9.7e-169 | 71.17 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DZW3 | 6.8e-234 | 99.49 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Momordica charantia OX=3673 GN=LOC1... | [more] |
A0A0A0L8Y0 | 3.9e-205 | 85.79 | Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 P... | [more] |
A0A6J1FQC1 | 4.0e-202 | 85.28 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucurbita moschata OX=3662 GN=LOC11... | [more] |
A0A5A7T3B3 | 2.0e-201 | 84.26 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695... | [more] |
A0A1S3BHA7 | 2.0e-201 | 84.26 | 3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983... | [more] |
Match Name | E-value | Identity | Description | |
AT4G24220.1 | 2.2e-184 | 75.26 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT4G24220.2 | 2.1e-182 | 75.00 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |
AT5G58750.1 | 6.2e-62 | 36.70 | NAD(P)-binding Rossmann-fold superfamily protein | [more] |