MS008656 (gene) Bitter gourd (TR) v1

Overview


Name	MS008656
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DUF1764 domain-containing protein
Location	scaffold4: 2883444 .. 2883833 (-)
RNA-Seq Expression	MS008656
Synteny	MS008656

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAAAAAAGAAATCTTCCAAAAAGCCTAAGGAGGTGCAAGAGAATGCTGCAACAAAAGAGGAAAAGCCCGATGCTGTGGTAAAAGAAGAAAAGCCCCCTTCAGCAAAGAAAAGTAGCGAGATTGATGAAATTTTTGCTGGGAAGAAGAGAAAGAAACCAGAAGTTGAAAAGGCCGAGAAGCCAAAACTAGATACATCTGATAGACCGAAAATGAAGAACAAGAAGAAGCGTGAGACTGTGTCTAAAGATGGCTTTTCAGATCCACCATCGCGGTCTAGAAAGAGGACAGGGGATGGACTTGCTATTTACACCGAAGAAGAATTGGGTTTTGGCAATGCAGATGCTGGAGGCACCCCACTTTGCCCATTCGACTGCTCTTGTTGCTTC

mRNA sequence

ATGCCAAAAAAGAAATCTTCCAAAAAGCCTAAGGAGGTGCAAGAGAATGCTGCAACAAAAGAGGAAAAGCCCGATGCTGTGGTAAAAGAAGAAAAGCCCCCTTCAGCAAAGAAAAGTAGCGAGATTGATGAAATTTTTGCTGGGAAGAAGAGAAAGAAACCAGAAGTTGAAAAGGCCGAGAAGCCAAAACTAGATACATCTGATAGACCGAAAATGAAGAACAAGAAGAAGCGTGAGACTGTGTCTAAAGATGGCTTTTCAGATCCACCATCGCGGTCTAGAAAGAGGACAGGGGATGGACTTGCTATTTACACCGAAGAAGAATTGGGTTTTGGCAATGCAGATGCTGGAGGCACCCCACTTTGCCCATTCGACTGCTCTTGTTGCTTC

Coding sequence (CDS)

ATGCCAAAAAAGAAATCTTCCAAAAAGCCTAAGGAGGTGCAAGAGAATGCTGCAACAAAAGAGGAAAAGCCCGATGCTGTGGTAAAAGAAGAAAAGCCCCCTTCAGCAAAGAAAAGTAGCGAGATTGATGAAATTTTTGCTGGGAAGAAGAGAAAGAAACCAGAAGTTGAAAAGGCCGAGAAGCCAAAACTAGATACATCTGATAGACCGAAAATGAAGAACAAGAAGAAGCGTGAGACTGTGTCTAAAGATGGCTTTTCAGATCCACCATCGCGGTCTAGAAAGAGGACAGGGGATGGACTTGCTATTTACACCGAAGAAGAATTGGGTTTTGGCAATGCAGATGCTGGAGGCACCCCACTTTGCCCATTCGACTGCTCTTGTTGCTTC

Protein sequence

MPKKKSSKKPKEVQENAATKEEKPDAVVKEEKPPSAKKSSEIDEIFAGKKRKKPEVEKAEKPKLDTSDRPKMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELGFGNADAGGTPLCPFDCSCCF

Homology

[Close]

BLAST of MS008656 vs. NCBI nr
Match: XP_022142164.1 (uncharacterized protein C6G9.01c [Momordica charantia])

HSP 1 Score: 243.4 bits (620), Expect = 1.1e-60
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKEEKPDAVVKEEKPPSAKKSSEIDEIFAGKKRKKPEVEKAE 60
           MPKKKSSKKPKEVQENAATKEEKPDAVVKEEKPPSAKKSSEIDEIFAGKKRKKPEVEKAE
Sbjct: 1   MPKKKSSKKPKEVQENAATKEEKPDAVVKEEKPPSAKKSSEIDEIFAGKKRKKPEVEKAE 60

Query: 61  KPKLDTSDRPKMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELGFGNADAGGTP 120
           KPKLDTSDRPKMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELGFGNADAGGTP
Sbjct: 61  KPKLDTSDRPKMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELGFGNADAGGTP 120

Query: 121 LCPFDCSCCF 131
           LCPFDCSCCF
Sbjct: 121 LCPFDCSCCF 130

[Close]

BLAST of MS008656 vs. NCBI nr
Match: KAA0064488.1 (DUF1764 domain-containing protein [Cucumis melo var. makuwa] >TYK20102.1 DUF1764 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 191.8 bits (486), Expect = 3.7e-45
Identity = 108/140 (77.14%), Postives = 117/140 (83.57%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKEEK---------PDAVVKEEKPPSAKKSSEIDEIFAGKKR 60
           MPKKKSSK PK+V ENA+TK+EK         P++VVKEEKPPS KK SEIDEIFAGKKR
Sbjct: 1   MPKKKSSKTPKKVPENASTKQEKSDAVVKGENPNSVVKEEKPPSVKKPSEIDEIFAGKKR 60

Query: 61  KKPEVEKAEKPKLDTSDRP-KMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELG 120
           KKPEV KAE+PKLD  DRP K KNKKK E  SK+GF DPPSR RK+TGDGLAIYTEEELG
Sbjct: 61  KKPEVAKAEEPKLDVQDRPKKTKNKKKDEAQSKNGFGDPPSRRRKKTGDGLAIYTEEELG 120

Query: 121 FGNADAGGTPLCPFDCSCCF 131
           FGNADAGGTPLCPFDC+CCF
Sbjct: 121 FGNADAGGTPLCPFDCNCCF 140

[Close]

BLAST of MS008656 vs. NCBI nr
Match: KAE8649903.1 (hypothetical protein Csa_012244 [Cucumis sativus])

HSP 1 Score: 184.1 bits (466), Expect = 7.6e-43
Identity = 103/140 (73.57%), Postives = 115/140 (82.14%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKEEK---------PDAVVKEEKPPSAKKSSEIDEIFAGKKR 60
           MPKKKSSK PK++ ENA+TK+EK         P++VVKEEKPPS KK SEIDEIFAGKKR
Sbjct: 1   MPKKKSSKTPKKIPENASTKQEKSNAVVKGENPNSVVKEEKPPSVKKHSEIDEIFAGKKR 60

Query: 61  KKPEVEKAEKPKLDTSDRP-KMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELG 120
           KKPEV K E+PKLD  DRP K KNKKK E  S++GF+DP SR RK+TGDGL IYTEEELG
Sbjct: 61  KKPEVGKTEEPKLDAQDRPKKTKNKKKDEAQSRNGFADPQSRRRKKTGDGLTIYTEEELG 120

Query: 121 FGNADAGGTPLCPFDCSCCF 131
           FGNADAGGTPLCPFDC+CCF
Sbjct: 121 FGNADAGGTPLCPFDCNCCF 140

[Close]

BLAST of MS008656 vs. NCBI nr
Match: XP_038897937.1 (uncharacterized protein C6G9.01c [Benincasa hispida] >XP_038897938.1 uncharacterized protein C6G9.01c [Benincasa hispida])

HSP 1 Score: 179.9 bits (455), Expect = 1.4e-41
Identity = 102/134 (76.12%), Postives = 113/134 (84.33%), Query Frame = 0


Query: 4   KKSSKKPKEVQENAATKE------EKPDAVVKEEKPPSAKKSSEIDEIFAGKKRKKPEVE 63
           KKSSK PK+VQENA TKE      EKP+  +KEEKPPS KK +EIDEIFAGKKRKKPEVE
Sbjct: 3   KKSSKTPKKVQENAFTKEENSNAAEKPNTAMKEEKPPSIKKHNEIDEIFAGKKRKKPEVE 62

Query: 64  KAEKPKLDTSDRP-KMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELGFGNADA 123
           KA++PKLDT  RP K+KNKK+  T SK+GF+DPPSR RK+TGDGL IYTEEELGFGN DA
Sbjct: 63  KAKEPKLDTPVRPKKIKNKKEDGTQSKNGFADPPSRRRKKTGDGLIIYTEEELGFGNPDA 122

Query: 124 GGTPLCPFDCSCCF 131
           GGTPLCPFDCSCCF
Sbjct: 123 GGTPLCPFDCSCCF 136

[Close]

BLAST of MS008656 vs. NCBI nr
Match: XP_022940022.1 (uncharacterized protein C6G9.01c [Cucurbita moschata])

HSP 1 Score: 176.8 bits (447), Expect = 1.2e-40
Identity = 101/140 (72.14%), Postives = 113/140 (80.71%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKEEKPDAVV---------KEEKPPSAKKSSEIDEIFAGKKR 60
           MPKKKSSK PKEV+E   TKEEK +AVV         KEEKPPS KK SEIDEIFAGKKR
Sbjct: 1   MPKKKSSKAPKEVKEKVITKEEKSNAVVKNEKPNSSIKEEKPPSVKKRSEIDEIFAGKKR 60

Query: 61  KKPEVEKAEKPKLDTSDRP-KMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELG 120
           KKPEV++A+KPKLD  DRP K+KNKK+ ET  ++ F DPPS+ RK+TGDGL IYTEEELG
Sbjct: 61  KKPEVQEAKKPKLDALDRPMKLKNKKRDETQPRNDFVDPPSQGRKKTGDGLKIYTEEELG 120

Query: 121 FGNADAGGTPLCPFDCSCCF 131
            G+ADAGGTPLCPFDCSCCF
Sbjct: 121 IGSADAGGTPLCPFDCSCCF 140

[Close]

BLAST of MS008656 vs. ExPASy Swiss-Prot
Match: Q92346 (Uncharacterized protein C6G9.01c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC6G9.01c PE=4 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 1.8e-07
Identity = 46/99 (46.46%), Postives = 54/99 (54.55%), Query Frame = 0


Query: 41  EIDEIFAGKKRKKPEVEKAEKPKLDTSDRPKMKNKKKRETVSK--------DGFSDPPSR 100
           EID+IFA KK   P  EK+   K + +  PK K  K + T S+        D F DP   
Sbjct: 2   EIDDIFASKK-ANPANEKSNDSKSE-AKAPK-KGAKTKSTPSRPKPTNNQDDLFLDPKGA 61

Query: 101 S-RKRTGDGLAIYTEEELGFGNADAGGTPLCPFDCSCCF 131
           S RKRT +G  +Y EEEL  G    GGTP CPFDC CCF
Sbjct: 62  SGRKRTEEGFLVYDEEELNIG--QGGGTPDCPFDCQCCF 95

[Close]

BLAST of MS008656 vs. ExPASy TrEMBL
Match: A0A6J1CKT3 (uncharacterized protein C6G9.01c OS=Momordica charantia OX=3673 GN=LOC111012350 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 5.1e-61
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKEEKPDAVVKEEKPPSAKKSSEIDEIFAGKKRKKPEVEKAE 60
           MPKKKSSKKPKEVQENAATKEEKPDAVVKEEKPPSAKKSSEIDEIFAGKKRKKPEVEKAE
Sbjct: 1   MPKKKSSKKPKEVQENAATKEEKPDAVVKEEKPPSAKKSSEIDEIFAGKKRKKPEVEKAE 60

Query: 61  KPKLDTSDRPKMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELGFGNADAGGTP 120
           KPKLDTSDRPKMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELGFGNADAGGTP
Sbjct: 61  KPKLDTSDRPKMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELGFGNADAGGTP 120

Query: 121 LCPFDCSCCF 131
           LCPFDCSCCF
Sbjct: 121 LCPFDCSCCF 130

[Close]

BLAST of MS008656 vs. ExPASy TrEMBL
Match: A0A5A7VA76 (DUF1764 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002260 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.8e-45
Identity = 108/140 (77.14%), Postives = 117/140 (83.57%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKEEK---------PDAVVKEEKPPSAKKSSEIDEIFAGKKR 60
           MPKKKSSK PK+V ENA+TK+EK         P++VVKEEKPPS KK SEIDEIFAGKKR
Sbjct: 1   MPKKKSSKTPKKVPENASTKQEKSDAVVKGENPNSVVKEEKPPSVKKPSEIDEIFAGKKR 60

Query: 61  KKPEVEKAEKPKLDTSDRP-KMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELG 120
           KKPEV KAE+PKLD  DRP K KNKKK E  SK+GF DPPSR RK+TGDGLAIYTEEELG
Sbjct: 61  KKPEVAKAEEPKLDVQDRPKKTKNKKKDEAQSKNGFGDPPSRRRKKTGDGLAIYTEEELG 120

Query: 121 FGNADAGGTPLCPFDCSCCF 131
           FGNADAGGTPLCPFDC+CCF
Sbjct: 121 FGNADAGGTPLCPFDCNCCF 140

[Close]

BLAST of MS008656 vs. ExPASy TrEMBL
Match: A0A6J1FIH3 (uncharacterized protein C6G9.01c OS=Cucurbita moschata OX=3662 GN=LOC111445781 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.9e-41
Identity = 101/140 (72.14%), Postives = 113/140 (80.71%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKEEKPDAVV---------KEEKPPSAKKSSEIDEIFAGKKR 60
           MPKKKSSK PKEV+E   TKEEK +AVV         KEEKPPS KK SEIDEIFAGKKR
Sbjct: 1   MPKKKSSKAPKEVKEKVITKEEKSNAVVKNEKPNSSIKEEKPPSVKKRSEIDEIFAGKKR 60

Query: 61  KKPEVEKAEKPKLDTSDRP-KMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELG 120
           KKPEV++A+KPKLD  DRP K+KNKK+ ET  ++ F DPPS+ RK+TGDGL IYTEEELG
Sbjct: 61  KKPEVQEAKKPKLDALDRPMKLKNKKRDETQPRNDFVDPPSQGRKKTGDGLKIYTEEELG 120

Query: 121 FGNADAGGTPLCPFDCSCCF 131
            G+ADAGGTPLCPFDCSCCF
Sbjct: 121 IGSADAGGTPLCPFDCSCCF 140

[Close]

BLAST of MS008656 vs. ExPASy TrEMBL
Match: A0A6J1IZA5 (uncharacterized protein C6G9.01c OS=Cucurbita maxima OX=3661 GN=LOC111481250 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.8e-40
Identity = 98/140 (70.00%), Postives = 113/140 (80.71%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKE---------EKPDAVVKEEKPPSAKKSSEIDEIFAGKKR 60
           MPKKKSSK  KEV+E   TKE         EKP++ +KEEKPPS KK SEIDEIFAGKKR
Sbjct: 1   MPKKKSSKAHKEVKEKVITKEEKSNSVVKNEKPNSSIKEEKPPSVKKRSEIDEIFAGKKR 60

Query: 61  KKPEVEKAEKPKLDTSDRP-KMKNKKKRETVSKDGFSDPPSRSRKRTGDGLAIYTEEELG 120
           KKPE+++A+KPK D  DRP K+KNKK+ ET  ++GF DPPS+SRK+TGDGL IYTEEELG
Sbjct: 61  KKPEIQEAKKPKQDALDRPMKLKNKKRDETQPRNGFVDPPSQSRKKTGDGLKIYTEEELG 120

Query: 121 FGNADAGGTPLCPFDCSCCF 131
            G+ADAGGTPLCPFDCSCCF
Sbjct: 121 IGSADAGGTPLCPFDCSCCF 140

[Close]

BLAST of MS008656 vs. ExPASy TrEMBL
Match: A0A2N9IL95 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS52633 PE=4 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.4e-29
Identity = 86/145 (59.31%), Postives = 100/145 (68.97%), Query Frame = 0


Query: 1   MPKKKSSKKPKEVQENAATKEEKPDA------------VVKEEKPPSAKK--SSEIDEIF 60
           MPKK SSKK K+++EN   +EEKP +            VV+EEKP SA+K  SSEIDEIF
Sbjct: 1   MPKKSSSKKAKQLKENNVVEEEKPSSAQKKAKQLKENNVVEEEKPSSAQKKASSEIDEIF 60

Query: 61  AGKKRKKPEVEKAEKPKLDTSDRPKMKNKKKRETVSKD-GFSDPPSRSRKRTGDGLAIYT 120
           AGKKRKK E EK EKP  D +  PK   KKK++  S D GF++PPSR RKRT DG  +YT
Sbjct: 61  AGKKRKKSEPEKTEKPNEDVAGNPKKMKKKKKDKESNDNGFAEPPSRPRKRTNDGFTVYT 120

Query: 121 EEELGFGNADAGGTPLCPFDCSCCF 131
           E+ELG   A AGGT LCPFDCSCCF
Sbjct: 121 EDELGIDKAAAGGTALCPFDCSCCF 145

[Close]

BLAST of MS008656 vs. TAIR 10
Match: AT5G11760.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1764, eukaryotic (InterPro:IPR013885); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 57.0 bits (136), Expect = 1.3e-08
Identity = 51/161 (31.68%), Postives = 69/161 (42.86%), Query Frame = 0


Query: 25  DAVVKEEK--PPSAKKSSEIDEIFAGKKRKKPEVE------------------KAEKPKL 84
           +AVV+  K  P   K   EID+IFAG+ ++ PEV+                  +AE  +L
Sbjct: 21  EAVVQRRKLVPKPGKIGKEIDDIFAGRSQRTPEVQNPESRDTPEAQVSESRDTRAEMNEL 80

Query: 85  ------DTSDRPKMK-----------------------------NKKKRETVSKDGFSDP 131
                 +T  RP+ +                             N++ R + S       
Sbjct: 81  NVGVNNNTESRPRTRILSNDTGPRSRTRRLLRTRRPLRTRTLRNNRESRPSSSIFNNDTE 140

The following BLAST results are available for this feature:

BLAST of MS008656 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142164.1	1.1e-60	100.00	uncharacterized protein C6G9.01c [Momordica charantia]	[more]
KAA0064488.1	3.7e-45	77.14	DUF1764 domain-containing protein [Cucumis melo var. makuwa] >TYK20102.1 DUF1764...	[more]
KAE8649903.1	7.6e-43	73.57	hypothetical protein Csa_012244 [Cucumis sativus]	[more]
XP_038897937.1	1.4e-41	76.12	uncharacterized protein C6G9.01c [Benincasa hispida] >XP_038897938.1 uncharacter...	[more]
XP_022940022.1	1.2e-40	72.14	uncharacterized protein C6G9.01c [Cucurbita moschata]	[more]

BLAST of MS008656 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q92346	1.8e-07	46.46	Uncharacterized protein C6G9.01c OS=Schizosaccharomyces pombe (strain 972 / ATCC...	[more]

BLAST of MS008656 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKT3	5.1e-61	100.00	uncharacterized protein C6G9.01c OS=Momordica charantia OX=3673 GN=LOC111012350 ...	[more]
A0A5A7VA76	1.8e-45	77.14	DUF1764 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1FIH3	5.9e-41	72.14	uncharacterized protein C6G9.01c OS=Cucurbita moschata OX=3662 GN=LOC111445781 P...	[more]
A0A6J1IZA5	3.8e-40	70.00	uncharacterized protein C6G9.01c OS=Cucurbita maxima OX=3661 GN=LOC111481250 PE=...	[more]
A0A2N9IL95	1.4e-29	59.31	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS52633 PE=4 SV=1	[more]

BLAST of MS008656 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G11760.1	1.3e-08	31.68	unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1764...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013885	Protein of unknown function DUF1764, eukaryotic	PFAM	PF08576	DUF1764	coord: 35..130 e-value: 1.2E-29 score: 103.1
IPR013885	Protein of unknown function DUF1764, eukaryotic	PANTHER	PTHR34066	GROWTH FACTOR 2	coord: 1..130
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 7..100
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..100

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008656.1	MS008656.1	mRNA