MS008624 (gene) Bitter gourd (TR) v1

BLAST of MS008624 vs. NCBI nr
Match: XP_022139449.1 ((S)-coclaurine N-methyltransferase-like isoform X1 [Momordica charantia])

HSP 1 Score: 721.1 bits (1860), Expect = 4.6e-204
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF
Sbjct: 3   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 62

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM
Sbjct: 63  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 122

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN
Sbjct: 123 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 182

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA
Sbjct: 183 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 242

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD
Sbjct: 243 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 302

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 348
           KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA
Sbjct: 303 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 349

BLAST of MS008624 vs. NCBI nr
Match: XP_022139456.1 ((S)-coclaurine N-methyltransferase-like isoform X2 [Momordica charantia])

HSP 1 Score: 645.6 bits (1664), Expect = 2.5e-181
Identity = 319/347 (91.93%), Postives = 319/347 (91.93%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF
Sbjct: 3   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 62

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM
Sbjct: 63  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 122

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN
Sbjct: 123 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 182

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA
Sbjct: 183 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 242

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE                            DDVSIINHWLLNGKHYAKTSEEWLKRMD
Sbjct: 243 YHFE----------------------------DDVSIINHWLLNGKHYAKTSEEWLKRMD 302

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 348
           KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA
Sbjct: 303 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 321

BLAST of MS008624 vs. NCBI nr
Match: XP_022139467.1 ((S)-coclaurine N-methyltransferase isoform X1 [Momordica charantia])

HSP 1 Score: 644.4 bits (1661), Expect = 5.5e-181
Identity = 306/346 (88.44%), Postives = 329/346 (95.09%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MAE+ Q+ Y+  VRLALASL+RNLLPDAV+R FTRLLLAARLRSGYKPSSQLQLSDLL F
Sbjct: 1   MAELMQLPYDVTVRLALASLERNLLPDAVVRRFTRLLLAARLRSGYKPSSQLQLSDLLHF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           +HSLREMPIAI+TDQPKAQHYELPTSFF LVLGK+LKYSCCYFNDKSS+LEDAEEAMLQM
Sbjct: 61  VHSLREMPIAIKTDQPKAQHYELPTSFFNLVLGKHLKYSCCYFNDKSSTLEDAEEAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNS TQKDYIEEQ  DL+LHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSATQKDYIEEQSRDLQLHN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNI+VADI+TFEMEAEYDRIFSI MFEHMKNYK+LL KISGWMKQDSLLFVH+FCHKVFA
Sbjct: 181 VNIIVADISTFEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHHFCHKVFA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE INED+WITRYFF  GTMPSANLLLYFQDDVSI++HWL+NGKHY++TSEEWLKRMD
Sbjct: 241 YHFENINEDDWITRYFFEGGTMPSANLLLYFQDDVSIVDHWLMNGKHYSQTSEEWLKRMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           +NI  IKPIMETTYGKDSA+KWTVYWRTFFIAVAELFGYN+GEEWM
Sbjct: 301 ENIALIKPIMETTYGKDSAVKWTVYWRTFFIAVAELFGYNNGEEWM 346

BLAST of MS008624 vs. NCBI nr
Match: XP_038898150.1 ((S)-coclaurine N-methyltransferase-like isoform X1 [Benincasa hispida] >XP_038898151.1 (S)-coclaurine N-methyltransferase-like isoform X1 [Benincasa hispida] >XP_038898152.1 (S)-coclaurine N-methyltransferase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 630.6 bits (1625), Expect = 8.2e-177
Identity = 297/346 (85.84%), Postives = 327/346 (94.51%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MAE+ QM Y+  VRLALASL+RNLLPDAV+R FTRLLLA+RLRSGYKPSSQLQLS+LL F
Sbjct: 1   MAELIQMPYDVTVRLALASLERNLLPDAVVRRFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           +HSL+EMPIAI+TD+PKAQHYE+PTSFF+LVLGKNLKYSCCYFNDKSS+LEDAE+AMLQM
Sbjct: 61  VHSLKEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQ YKNCRVTGICNSTTQK YIE+QC DL+LHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCRVTGICNSTTQKAYIEDQCQDLQLHN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNI+VADI+TFEMEAEYDRIFSI MFEHMKNYK+LL KIS WMKQDSLLFVH+FCHKVFA
Sbjct: 181 VNIIVADISTFEMEAEYDRIFSIEMFEHMKNYKDLLKKISVWMKQDSLLFVHHFCHKVFA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE +NED+WITRYFF  GTMPSAN+LLYFQDDVSI++HWL+NGKHY++TSEEWLKRMD
Sbjct: 241 YHFEDVNEDDWITRYFFEGGTMPSANVLLYFQDDVSIVDHWLVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           KN  SIKPIM  TYGKDSA+KWTVYWRTFFIAVAELFGYN+GEEWM
Sbjct: 301 KNADSIKPIMAATYGKDSAVKWTVYWRTFFIAVAELFGYNNGEEWM 346

BLAST of MS008624 vs. NCBI nr
Match: KAA0064517.1 ((S)-coclaurine N-methyltransferase-like [Cucumis melo var. makuwa] >TYK20073.1 (S)-coclaurine N-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 625.2 bits (1611), Expect = 3.5e-175
Identity = 293/346 (84.68%), Postives = 327/346 (94.51%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MA++ QM Y+ +VRLALASL+RNLLPDAV+R FTRLLLA+RLRSGYKPSSQLQLS+LL F
Sbjct: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           +HSL EMPIAI+TD+PKAQHYE+PTSFF+LVLGKNLKYSCCYFNDKSS+LEDAE+AMLQM
Sbjct: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQ YKNC VTGICNS TQK YIE++C DL+LHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNI+VADI+T+EMEAEYDRIFSI MFEHMKNYK+LL KISGWMKQDSLLFVHYFCHKVFA
Sbjct: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE +NED+WITRYFF  GTMPS+NLLLYFQ++VSI++HW +NGKHY++TSEEWLKRMD
Sbjct: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           KNI SIKPIM TTYGKDSA+KWTVYWRTFFIAVAELFGYN+GEEWM
Sbjct: 301 KNIASIKPIMATTYGKDSAVKWTVYWRTFFIAVAELFGYNNGEEWM 346

BLAST of MS008624 vs. ExPASy Swiss-Prot
Match: Q5C9L6 ((S)-coclaurine N-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.6e-101
Identity = 174/335 (51.94%), Postives = 238/335 (71.04%), Query Frame = 0

Query: 17  LASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRFLHSLREMPIAIETDQP 76
           L  L+  L+PD  I+   R+ L  RL+ G K + + Q++ L+   HSLR+M IA E +  
Sbjct: 19  LKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLTHSLRQMKIATEVETL 78

Query: 77  KAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQMYCERSQLKDGHTVLDV 136
             Q YE+P  F +++ G NLK SCCYF + S++L++AE AML++YCER+Q+KDGH+VLD+
Sbjct: 79  DDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYCERAQIKDGHSVLDL 138

Query: 137 GCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHNVNIVVADINTFEMEAE 196
           GCG G+L+LY+AQ YKN RVT + NS +QK++IEE+     L NV +++ADI T +M   
Sbjct: 139 GCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNVEVLLADITTHKMPDT 198

Query: 197 YDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFAYHFEGINEDNWITRYF 256
           YDRI  + +FEHMKNY+ LL KI  WM +D LLFV + CHK FAYH+E I+ED+W T Y 
Sbjct: 199 YDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDEDDWFTEYV 258

Query: 257 FAYGTM--PSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMDKNIGSIKPIMETTY 316
           F  GTM  PSA+  LYFQDDVS++NHW L+GKH+++T+EEWLKR+D N+  IKP+  T  
Sbjct: 259 FPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELIKPMFVTIT 318

Query: 317 G--KDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 348
           G  +  A+K   YWR F ++  E+FGYN+GEEWMA
Sbjct: 319 GQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMA 353

BLAST of MS008624 vs. ExPASy Swiss-Prot
Match: C3SBW0 (Pavine N-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 6.9e-94
Identity = 162/347 (46.69%), Postives = 239/347 (68.88%), Query Frame = 0

Query: 3   EIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRFLH 62
           E KQ    A   L +  ++   + D  IR   ++ +  RL+ GYKP+ + QL+ L+ F  
Sbjct: 2   ETKQTKKEAVANL-IKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 61

Query: 63  SLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQMYC 122
           SL+ M +A E D   A+ YE+P  F  ++ GK LK+S  YF D+S++L+++E  M+ +YC
Sbjct: 62  SLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYC 121

Query: 123 ERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHNVN 182
           ER+Q+KDG ++LD+GCG GSL+L++AQ Y+ C+VTGI NS +QK++I +QC  L+L NV 
Sbjct: 122 ERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVE 181

Query: 183 IVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFAYH 242
           I++ D+  FE E  YDRIF++ + EHMKNY+  L K+S W+ Q  LLFV + CHKVFAY 
Sbjct: 182 IILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQ 241

Query: 243 FEGINEDNWITRYFFAYGT--MPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 302
           +E ++ED+W T Y F  GT  M S+++LLYFQ+DVS++NHW L+GKH +   ++WLKR+D
Sbjct: 242 YEPLDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLD 301

Query: 303 KNIGSIKPIMETTYG-KDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
            NI  +K I E+ YG K+ A+K+  YWR F IA ++++  N+GEEWM
Sbjct: 302 DNIDEVKEIFESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWM 347

BLAST of MS008624 vs. ExPASy Swiss-Prot
Match: Q7XB08 ((S)-coclaurine N-methyltransferase OS=Papaver somniferum OX=3469 GN=CNMT PE=1 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.0e-92
Identity = 163/333 (48.95%), Postives = 231/333 (69.37%), Query Frame = 0

Query: 17  LASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRFLHSLREMPIAIETDQP 76
           L +++  L+PD  IR   R+ L  RL+ GYK + + QLS LL  +HSL+ M +A E +  
Sbjct: 10  LRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENL 69

Query: 77  KAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQMYCERSQLKDGHTVLDV 136
             + YE P  F ++  G N+K S  Y+ D+S++L++AE AML +Y ER+Q+KDG +VLD+
Sbjct: 70  DLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDL 129

Query: 137 GCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHNVNIVVADINTFEMEAE 196
           GCG G+++L+ A  +K C+ TG+ +S  QKDYIE +C +L+L NV +++ADI T+E E  
Sbjct: 130 GCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETEER 189

Query: 197 YDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFAYHFEGINEDNWITRYF 256
           +DRIF++ + EHMKNY+ LL KIS WMK D LLFV + CHK  AYH+E ++ ++W T Y 
Sbjct: 190 FDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYI 249

Query: 257 FAYG--TMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMDKNIGSIKPIMET-T 316
           F  G  T+ SA++LLYFQDDVS++N W L+GKHY+++ EEWLK MDKNI   K IM + T
Sbjct: 250 FPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIMRSIT 309

Query: 317 YGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
             +  A+K   +WR F +  AELFGY +GEEWM
Sbjct: 310 KTEKEAIKLLNFWRIFCMCGAELFGYKNGEEWM 342

BLAST of MS008624 vs. ExPASy Swiss-Prot
Match: C3SBS8 ((S)-tetrahydroprotoberberine N-methyltransferase OS=Eschscholzia californica OX=3467 PE=1 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.7e-84
Identity = 147/309 (47.57%), Postives = 211/309 (68.28%), Query Frame = 0

Query: 41  RLRSGYKPSSQLQLSDLLRFLHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSC 100
           R+  GYKP+ + QL+  L F+  L+EM ++ E D    + YELPT+F E V GK +K SC
Sbjct: 33  RIEWGYKPTHEKQLAFNLDFIKGLKEMVMSGEIDTMNKETYELPTAFLEAVFGKTVKQSC 92

Query: 101 CYFNDKSSSLEDAEEAMLQMYCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGIC 160
           CYF D++S++++AEEA  ++YCER+Q+KDG TVLD+GCG G L LYIA+ YKNC VTG+ 
Sbjct: 93  CYFKDENSTIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIAEKYKNCHVTGLT 152

Query: 161 NSTTQKDYIEEQCWDLELHNVNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKIS 220
           NS  Q +YIE+Q   LEL NV+++ AD+  F+ +  YDRI  +   EHMKN +  + K+S
Sbjct: 153 NSKAQANYIEQQAEKLELTNVDVIFADVTKFDTDKTYDRILVVETIEHMKNIQLFMKKLS 212

Query: 221 GWMKQDSLLFVHYFCHKVFAYHFEGINEDNWITRYFFAYG--TMPSANLLLYFQDDVSII 280
            WM +DSLLFV +  HK F ++FE ++ED+W + + F  G  T+ S++ LLYFQDDVS +
Sbjct: 213 TWMTEDSLLFVDHISHKTFNHNFEALDEDDWYSGFIFPKGCVTILSSSTLLYFQDDVSAL 272

Query: 281 NHWLLNGKHYAKTSEEWLKRMDKNIGSIKPIMETTYGKDSALKWTV-YWRTFFIAVAELF 340
           +HW++NG H A++ E W K++D+ I + + I+E   G   A+   + + RTF I   E F
Sbjct: 273 DHWVVNGMHMARSVEAWRKKLDETIEAAREILEPGLGSKEAVNQVITHIRTFCIGGYEQF 332

Query: 341 GYNDGEEWM 347
            YN+GEEWM
Sbjct: 333 SYNNGEEWM 341

BLAST of MS008624 vs. ExPASy Swiss-Prot
Match: C3SBU4 (Probable (S)-tetrahydroprotoberberine N-methyltransferase 2 OS=Papaver bracteatum OX=215227 PE=2 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.1e-80
Identity = 151/334 (45.21%), Postives = 219/334 (65.57%), Query Frame = 0

Query: 17  LASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRFLHSLREMPIAIETDQP 76
           L  L R  + D  ++   +  L  RL+ GYK S Q QLS  L F++SL++M ++ + +  
Sbjct: 16  LGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFINSLKKMGMSGQVEAF 75

Query: 77  KAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQMYCERSQLKDGHTVLDV 136
             + YELPT  FE   GK++K S CYF  +SS++++AEEA  ++YCER+Q+KDG TVLD+
Sbjct: 76  TNEVYELPTECFEAAYGKSMKLSGCYFKHESSTIDEAEEASHELYCERAQIKDGQTVLDI 135

Query: 137 GCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHNVNIVVADINTFEMEAE 196
           GCG G L LY+AQ YKNC VTG+ NS  Q +YI +Q   L L NV++++AD+  +E +  
Sbjct: 136 GCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVTQYESDKT 195

Query: 197 YDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFAYHFEGINEDNWITRYF 256
           YDRI  IG+ EHMKN +  + K+S WM +DSLLFV + CHK F + FE ++ED+W + Y 
Sbjct: 196 YDRILVIGVVEHMKNMQLFIKKLSTWMAEDSLLFVDHSCHKTFNHFFEALDEDDWYSGYI 255

Query: 257 FAYG--TMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMDKNIGSIKPI-METT 316
           F  G  T  SA+ LLYFQDDVS+++HW++NG H+A+T + W K++DKN+ ++K I +   
Sbjct: 256 FPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEAVKEILLPGL 315

Query: 317 YGKDSALKWTV-YWRTFFIAVAELFGYNDGEEWM 347
            G   A+   + + RT  +     F  NDG+EWM
Sbjct: 316 GGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWM 349

BLAST of MS008624 vs. ExPASy TrEMBL
Match: A0A6J1CD23 ((S)-coclaurine N-methyltransferase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010373 PE=4 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.2e-204
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF
Sbjct: 3   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 62

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM
Sbjct: 63  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 122

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN
Sbjct: 123 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 182

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA
Sbjct: 183 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 242

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD
Sbjct: 243 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 302

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 348
           KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA
Sbjct: 303 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 349

BLAST of MS008624 vs. ExPASy TrEMBL
Match: A0A6J1CCQ7 ((S)-coclaurine N-methyltransferase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010373 PE=4 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.2e-181
Identity = 319/347 (91.93%), Postives = 319/347 (91.93%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF
Sbjct: 3   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 62

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM
Sbjct: 63  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 122

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN
Sbjct: 123 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 182

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA
Sbjct: 183 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 242

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE                            DDVSIINHWLLNGKHYAKTSEEWLKRMD
Sbjct: 243 YHFE----------------------------DDVSIINHWLLNGKHYAKTSEEWLKRMD 302

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 348
           KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA
Sbjct: 303 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWMA 321

BLAST of MS008624 vs. ExPASy TrEMBL
Match: A0A6J1CE09 ((S)-coclaurine N-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010387 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.7e-181
Identity = 306/346 (88.44%), Postives = 329/346 (95.09%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MAE+ Q+ Y+  VRLALASL+RNLLPDAV+R FTRLLLAARLRSGYKPSSQLQLSDLL F
Sbjct: 1   MAELMQLPYDVTVRLALASLERNLLPDAVVRRFTRLLLAARLRSGYKPSSQLQLSDLLHF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           +HSLREMPIAI+TDQPKAQHYELPTSFF LVLGK+LKYSCCYFNDKSS+LEDAEEAMLQM
Sbjct: 61  VHSLREMPIAIKTDQPKAQHYELPTSFFNLVLGKHLKYSCCYFNDKSSTLEDAEEAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNS TQKDYIEEQ  DL+LHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSATQKDYIEEQSRDLQLHN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNI+VADI+TFEMEAEYDRIFSI MFEHMKNYK+LL KISGWMKQDSLLFVH+FCHKVFA
Sbjct: 181 VNIIVADISTFEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHHFCHKVFA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE INED+WITRYFF  GTMPSANLLLYFQDDVSI++HWL+NGKHY++TSEEWLKRMD
Sbjct: 241 YHFENINEDDWITRYFFEGGTMPSANLLLYFQDDVSIVDHWLMNGKHYSQTSEEWLKRMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           +NI  IKPIMETTYGKDSA+KWTVYWRTFFIAVAELFGYN+GEEWM
Sbjct: 301 ENIALIKPIMETTYGKDSAVKWTVYWRTFFIAVAELFGYNNGEEWM 346

BLAST of MS008624 vs. ExPASy TrEMBL
Match: A0A5A7VC26 ((S)-coclaurine N-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001960 PE=4 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 1.7e-175
Identity = 293/346 (84.68%), Postives = 327/346 (94.51%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MA++ QM Y+ +VRLALASL+RNLLPDAV+R FTRLLLA+RLRSGYKPSSQLQLS+LL F
Sbjct: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           +HSL EMPIAI+TD+PKAQHYE+PTSFF+LVLGKNLKYSCCYFNDKSS+LEDAE+AMLQM
Sbjct: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQ YKNC VTGICNS TQK YIE++C DL+LHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNI+VADI+T+EMEAEYDRIFSI MFEHMKNYK+LL KISGWMKQDSLLFVHYFCHKVFA
Sbjct: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE +NED+WITRYFF  GTMPS+NLLLYFQ++VSI++HW +NGKHY++TSEEWLKRMD
Sbjct: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           KNI SIKPIM TTYGKDSA+KWTVYWRTFFIAVAELFGYN+GEEWM
Sbjct: 301 KNIASIKPIMATTYGKDSAVKWTVYWRTFFIAVAELFGYNNGEEWM 346

BLAST of MS008624 vs. ExPASy TrEMBL
Match: A0A0A0L426 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G646310 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.2e-175
Identity = 293/346 (84.68%), Postives = 327/346 (94.51%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           MA++ Q+ Y+A V LALASL+RNLLPDA+IR FTRLLLA+RLRSGYKPSSQLQLS+LL F
Sbjct: 1   MAKLIQIPYDATVHLALASLERNLLPDAIIRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           +HSLREMPIAI+TD+PKAQHYE+PTSFF+LVLGKNLKYSCCYFNDKSS+LEDAE+AMLQM
Sbjct: 61  VHSLREMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQ YKNC VTGICNS TQK YIE++C DL+LHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           VNI+VADI+T+EMEAEYDRIFSI MFEHMKNYK+LL KISGWMKQDSLLFVHYFCHKVFA
Sbjct: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE +N+D+WITRYFF  GTM S+NLLLYFQDDVSI++HWL+NGKHY++TSEEWLKRMD
Sbjct: 241 YHFEDVNDDDWITRYFFEGGTMLSSNLLLYFQDDVSIVDHWLVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           +NI SIKPIM TTYGKDSA+KWTVYWRTFFIAVAELFGYN+GEEWM
Sbjct: 301 ENIASIKPIMATTYGKDSAVKWTVYWRTFFIAVAELFGYNNGEEWM 346

BLAST of MS008624 vs. TAIR 10
Match: AT4G33110.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-149
Identity = 241/346 (69.65%), Postives = 297/346 (85.84%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           M +I  + Y A+V++ L  L+ NLLPD VIR  TRLLLA RLRSGYKP++++QLSDLLRF
Sbjct: 1   MEKIIDVAYGASVKVGLTLLEMNLLPDIVIRRLTRLLLAGRLRSGYKPTAEMQLSDLLRF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           + S+++MPIAI T++PK QHYELPT+FFELVLG+N+KYS CYF++ SSSLEDAEEA+L +
Sbjct: 61  VDSIKKMPIAIHTEKPKTQHYELPTAFFELVLGRNMKYSSCYFSNDSSSLEDAEEAILAL 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCER++++DG +VLD+GCGWGSLSLYIA+ Y  C++TGICNS TQK +I+E+C  L L N
Sbjct: 121 YCERAKVEDGQSVLDIGCGWGSLSLYIARKYSKCKLTGICNSKTQKAFIDEKCRKLGLQN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           + I+VADI+TFE E  YDRIFSI MFEHMKNY  LL KI  WMK+DSLLFVHYFCHK FA
Sbjct: 181 IEIIVADISTFEHEGTYDRIFSIEMFEHMKNYGELLKKIGKWMKEDSLLFVHYFCHKTFA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE +N+D+WITR+FF+ GTMPSA+LLLYFQ++VSI++HWL++G HYAKTSEEWLKRMD
Sbjct: 241 YHFEDVNDDDWITRHFFSGGTMPSADLLLYFQENVSIVDHWLVSGTHYAKTSEEWLKRMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           K I ++K IME TYGK+ A+KW VYWRTFFIAVAELFGYN+GEEWM
Sbjct: 301 KEIVAVKKIMEVTYGKEEAVKWMVYWRTFFIAVAELFGYNNGEEWM 346

BLAST of MS008624 vs. TAIR 10
Match: AT4G33110.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 526.6 bits (1355), Expect = 1.6e-149
Identity = 241/346 (69.65%), Postives = 297/346 (85.84%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           M +I  + Y A+V++ L  L+ NLLPD VIR  TRLLLA RLRSGYKP++++QLSDLLRF
Sbjct: 1   MEKIIDVAYGASVKVGLTLLEMNLLPDIVIRRLTRLLLAGRLRSGYKPTAEMQLSDLLRF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           + S+++MPIAI T++PK QHYELPT+FFELVLG+N+KYS CYF++ SSSLEDAEEA+L +
Sbjct: 61  VDSIKKMPIAIHTEKPKTQHYELPTAFFELVLGRNMKYSSCYFSNDSSSLEDAEEAILAL 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           YCER++++DG +VLD+GCGWGSLSLYIA+ Y  C++TGICNS TQK +I+E+C  L L N
Sbjct: 121 YCERAKVEDGQSVLDIGCGWGSLSLYIARKYSKCKLTGICNSKTQKAFIDEKCRKLGLQN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           + I+VADI+TFE E  YDRIFSI MFEHMKNY  LL KI  WMK+DSLLFVHYFCHK FA
Sbjct: 181 IEIIVADISTFEHEGTYDRIFSIEMFEHMKNYGELLKKIGKWMKEDSLLFVHYFCHKTFA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE +N+D+WITR+FF+ GTMPSA+LLLYFQ++VSI++HWL++G HYAKTSEEWLKRMD
Sbjct: 241 YHFEDVNDDDWITRHFFSGGTMPSADLLLYFQENVSIVDHWLVSGTHYAKTSEEWLKRMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           K I ++K IME TYGK+ A+KW VYWRTFFIAVAELFGYN+GEEWM
Sbjct: 301 KEIVAVKKIMEVTYGKEEAVKWMVYWRTFFIAVAELFGYNNGEEWM 346

BLAST of MS008624 vs. TAIR 10
Match: AT4G33120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 501.9 bits (1291), Expect = 4.1e-142
Identity = 230/346 (66.47%), Postives = 288/346 (83.24%), Query Frame = 0

Query: 1   MAEIKQMLYNAAVRLALASLDRNLLPDAVIRIFTRLLLAARLRSGYKPSSQLQLSDLLRF 60
           M +I  + Y A+V+  L  L++NLLPD VIR   RLLLA+RLRS YKP++++QLSDLLRF
Sbjct: 1   MEKIIDVAYGASVKAGLTLLEKNLLPDLVIRRLIRLLLASRLRSVYKPTAEMQLSDLLRF 60

Query: 61  LHSLREMPIAIETDQPKAQHYELPTSFFELVLGKNLKYSCCYFNDKSSSLEDAEEAMLQM 120
           + SL++MPIAI T+  K QHYELPT+FFE  LG+N+KYSCCY+++ SSSLE+A EAML +
Sbjct: 61  VDSLKKMPIAINTETAKTQHYELPTAFFEHALGRNIKYSCCYYSNDSSSLEEAGEAMLAL 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
            CER++++DG +VLD+GCGWGSL+LYIA+ Y  C++TGICNS TQK +I+E+C  L L N
Sbjct: 121 SCERAKVEDGQSVLDIGCGWGSLTLYIARKYSKCKLTGICNSKTQKAFIDEKCRKLGLQN 180

Query: 181 VNIVVADINTFEMEAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFCHKVFA 240
           V I+ ADI+TFE E  YDRIFSI MFEHMKNY  LL KI  WMK+DSLLFVHY CHK +A
Sbjct: 181 VEIIAADISTFEHEGTYDRIFSIEMFEHMKNYGELLKKIGNWMKEDSLLFVHYLCHKTYA 240

Query: 241 YHFEGINEDNWITRYFFAYGTMPSANLLLYFQDDVSIINHWLLNGKHYAKTSEEWLKRMD 300
           YHFE +N+D+WITR+FF+ GTMPSA+LLLYFQ++VSI++HWL+NG HYAKTSEEWLK MD
Sbjct: 241 YHFEDVNDDDWITRHFFSGGTMPSADLLLYFQENVSIMDHWLVNGTHYAKTSEEWLKGMD 300

Query: 301 KNIGSIKPIMETTYGKDSALKWTVYWRTFFIAVAELFGYNDGEEWM 347
           K I ++K IME TYGK+ A+KWTVYWRTFFIA+AELF YN+G+EWM
Sbjct: 301 KEIVAVKEIMEVTYGKEEAVKWTVYWRTFFIALAELFAYNNGDEWM 346

BLAST of MS008624 vs. TAIR 10
Match: AT3G18000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 2.1e-05
Identity = 33/116 (28.45%), Postives = 52/116 (44.83%), Query Frame = 0

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           + E+  LK G  VLDVGCG G    Y+A+ + +  V GI  S     +  E+   L   +
Sbjct: 274 FVEKMNLKPGQKVLDVGCGIGGGDFYMAEKF-DVHVVGIDLSVNMISFALERAIGLSC-S 333

Query: 181 VNIVVADINTFEM-EAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFC 236
           V   VAD  T    +  +D I+S     H+++   L      W+K    + +  +C
Sbjct: 334 VEFEVADCTTKHYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGKVLISDYC 387

BLAST of MS008624 vs. TAIR 10
Match: AT1G48600.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 2.8e-05
Identity = 33/116 (28.45%), Postives = 53/116 (45.69%), Query Frame = 0

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSTTQKDYIEEQCWDLELHN 180
           +  +  LK G  VLDVGCG G    Y+A+N+ +  V GI  S     +  E+   L+  +
Sbjct: 274 FVAKMDLKPGQKVLDVGCGIGGGDFYMAENF-DVHVVGIDLSVNMISFALERAIGLKC-S 333

Query: 181 VNIVVADINTFEM-EAEYDRIFSIGMFEHMKNYKNLLNKISGWMKQDSLLFVHYFC 236
           V   VAD  T    +  +D I+S     H+++   L      W+K    + +  +C
Sbjct: 334 VEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGKVLITDYC 387