MS008621 (gene) Bitter gourd (TR) v1

Overview


Name	MS008621
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	17.1 kDa class II heat shock protein-like
Location	scaffold4: 2675220 .. 2675696 (+)
RNA-Seq Expression	MS008621
Synteny	MS008621

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGACAACCAAAGTGGGCGGTTTCGACCCCATATTCCTCAACGTCCTCCACGACCTGATCGACTTCACGGACGAGCCTGGGCAGAGCGGCCACCACGCGCCGTCACGCGCGTACGTGCGAGACGCGAAAGCCATGGCGGCGACTCCGGCCGACGTCGTCGAATACCCGAACTGCTACCAGTTCGCGATCGACATGCCGGGGCTGAAGCCGGACCTAATCAAGGTCCAGATCGAGGAGAACCAGCAGCTGGTGGTGAGCGGCGAGAGGAAGAGGGAAAGCGAGAAGAATAAGGAGGGCAAGTACGTCAAAATGGAGAGGAGGCTGGGGAAGTACTTGAAGAAATTTCCGCTGCCGGAGACCGCCGACGCCGAGAAGGTGTCGGCGGTGTACCAGGACGGGGTGCTGTCGGTGACGGTGGAGAAGAGGCCGCCGCCGGAGCCCAAGAAGGCCAAGACTATTGAGGTTCAAGTGGCC

mRNA sequence

ATGGAGACAACCAAAGTGGGCGGTTTCGACCCCATATTCCTCAACGTCCTCCACGACCTGATCGACTTCACGGACGAGCCTGGGCAGAGCGGCCACCACGCGCCGTCACGCGCGTACGTGCGAGACGCGAAAGCCATGGCGGCGACTCCGGCCGACGTCGTCGAATACCCGAACTGCTACCAGTTCGCGATCGACATGCCGGGGCTGAAGCCGGACCTAATCAAGGTCCAGATCGAGGAGAACCAGCAGCTGGTGGTGAGCGGCGAGAGGAAGAGGGAAAGCGAGAAGAATAAGGAGGGCAAGTACGTCAAAATGGAGAGGAGGCTGGGGAAGTACTTGAAGAAATTTCCGCTGCCGGAGACCGCCGACGCCGAGAAGGTGTCGGCGGTGTACCAGGACGGGGTGCTGTCGGTGACGGTGGAGAAGAGGCCGCCGCCGGAGCCCAAGAAGGCCAAGACTATTGAGGTTCAAGTGGCC

Coding sequence (CDS)

ATGGAGACAACCAAAGTGGGCGGTTTCGACCCCATATTCCTCAACGTCCTCCACGACCTGATCGACTTCACGGACGAGCCTGGGCAGAGCGGCCACCACGCGCCGTCACGCGCGTACGTGCGAGACGCGAAAGCCATGGCGGCGACTCCGGCCGACGTCGTCGAATACCCGAACTGCTACCAGTTCGCGATCGACATGCCGGGGCTGAAGCCGGACCTAATCAAGGTCCAGATCGAGGAGAACCAGCAGCTGGTGGTGAGCGGCGAGAGGAAGAGGGAAAGCGAGAAGAATAAGGAGGGCAAGTACGTCAAAATGGAGAGGAGGCTGGGGAAGTACTTGAAGAAATTTCCGCTGCCGGAGACCGCCGACGCCGAGAAGGTGTCGGCGGTGTACCAGGACGGGGTGCTGTCGGTGACGGTGGAGAAGAGGCCGCCGCCGGAGCCCAAGAAGGCCAAGACTATTGAGGTTCAAGTGGCC

Protein sequence

METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA

Homology

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BLAST of MS008621 vs. NCBI nr
Match: XP_022135195.1 (17.1 kDa class II heat shock protein-like [Momordica charantia])

HSP 1 Score: 316.6 bits (810), Expect = 1.2e-82
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60
           METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY
Sbjct: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60

Query: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120
           QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE
Sbjct: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120

Query: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA
Sbjct: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 159

[Close]

BLAST of MS008621 vs. NCBI nr
Match: XP_004141335.2 (17.3 kDa class II heat shock protein [Cucumis sativus])

HSP 1 Score: 256.5 bits (654), Expect = 1.5e-64
Identity = 128/159 (80.50%), Postives = 143/159 (89.94%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60
           ME  K+ GFDP+FLN LHDL+DF+DEPGQ  HHAPSRAY+RDAKAMAATPADVVEYPN Y
Sbjct: 51  MEVAKMPGFDPLFLNALHDLLDFSDEPGQGSHHAPSRAYLRDAKAMAATPADVVEYPNSY 110

Query: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120
           QF IDMPGL  D IKV++E+  QLVVSGERKRESEK KEGK+V+MERRLGKYLKKF LPE
Sbjct: 111 QFTIDMPGLTSDQIKVKVEDG-QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPE 170

Query: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           TADA+KVSA Y+DGVLSVTVEK+PPPEPKKAK+IEV+VA
Sbjct: 171 TADADKVSAAYRDGVLSVTVEKKPPPEPKKAKSIEVRVA 208

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BLAST of MS008621 vs. NCBI nr
Match: XP_008452768.1 (PREDICTED: 17.3 kDa class II heat shock protein-like [Cucumis melo] >KAA0064522.1 17.3 kDa class II heat shock protein-like [Cucumis melo var. makuwa] >TYK20068.1 17.3 kDa class II heat shock protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 255.4 bits (651), Expect = 3.3e-64
Identity = 128/159 (80.50%), Postives = 143/159 (89.94%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60
           M+  K+ GFDP+FLN LHDL+DF+DEPGQS HHAPSRAY+RDAKAMAATPADVVEYPN Y
Sbjct: 1   MDVAKM-GFDPLFLNALHDLLDFSDEPGQSSHHAPSRAYLRDAKAMAATPADVVEYPNSY 60

Query: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120
           QF IDMPGLK D IKV++E+  QLVVSGERKRESEK KEGK+V+MERRLGKYLKKF LPE
Sbjct: 61  QFTIDMPGLKSDQIKVKVEDG-QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFELPE 120

Query: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           TAD +K+SA YQDGVLSVTVEK+PPPEPKKAKTIE+ VA
Sbjct: 121 TADTDKISAAYQDGVLSVTVEKKPPPEPKKAKTIEIHVA 157

[Close]

BLAST of MS008621 vs. NCBI nr
Match: XP_022982419.1 (17.1 kDa class II heat shock protein-like [Cucurbita maxima])

HSP 1 Score: 254.6 bits (649), Expect = 5.6e-64
Identity = 130/151 (86.09%), Postives = 137/151 (90.73%), Query Frame = 0


Query: 8   GFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMP 67
           G DPIFLN LHDL+DFTDEPGQS HH PSRAYVRDAKAMAATPADVVEYPN YQF+IDMP
Sbjct: 7   GLDPIFLNALHDLLDFTDEPGQSTHHGPSRAYVRDAKAMAATPADVVEYPNAYQFSIDMP 66

Query: 68  GLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKV 127
           GLK D IKVQIE+N QLVV+GERKRESEK KEGKYV MERRLGKYLKKF LPE AD +KV
Sbjct: 67  GLKMDQIKVQIEDN-QLVVTGERKRESEKAKEGKYVTMERRLGKYLKKFVLPEAADVDKV 126

Query: 128 SAVYQDGVLSVTVEKRPPPEPKKAKTIEVQV 159
           SA YQDGVLSVTVEK+PPPEPKKAKTIEV+V
Sbjct: 127 SAAYQDGVLSVTVEKKPPPEPKKAKTIEVRV 156

[Close]

BLAST of MS008621 vs. NCBI nr
Match: KAE8649916.1 (hypothetical protein Csa_012089 [Cucumis sativus])

HSP 1 Score: 252.3 bits (643), Expect = 2.8e-63
Identity = 125/157 (79.62%), Postives = 141/157 (89.81%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60
           ME  K+ GFDP+FLN LHDL+DF+DEPGQ  HHAPSRAY+RDAKAMAATPADVVEYPN Y
Sbjct: 1   MEVAKMPGFDPLFLNALHDLLDFSDEPGQGSHHAPSRAYLRDAKAMAATPADVVEYPNSY 60

Query: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120
           QF IDMPGL  D IKV++E+  QLVVSGERKRESEK KEGK+V+MERRLGKYLKKF LPE
Sbjct: 61  QFTIDMPGLTSDQIKVKVEDG-QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPE 120

Query: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQ 158
           TADA+KVSA Y+DGVLSVTVEK+PPPEPKKAK+IE++
Sbjct: 121 TADADKVSAAYRDGVLSVTVEKKPPPEPKKAKSIELK 156

[Close]

BLAST of MS008621 vs. ExPASy Swiss-Prot
Match: P46516 (17.9 kDa class II heat shock protein OS=Helianthus annuus OX=4232 GN=HSP17.9 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 7.9e-45
Identity = 99/163 (60.74%), Postives = 124/163 (76.07%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDE---PGQSGHHAPSRAYVRDAKAMAATPADVVEYP 60
           M+   + GFDP+  N LH +++ TD+     +S +  PSRAYVRDA+AMAATPADV E P
Sbjct: 1   MDIDSLMGFDPLLRN-LHYILEATDDNTTGNKSNNSGPSRAYVRDARAMAATPADVKECP 60

Query: 61  NCYQFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEG-KYVKMERRLGKYLKKF 120
           N Y F +DMPGLK   IKVQ+E +  LV+SG+R RE E  KEG KYV+MERR+GK++KKF
Sbjct: 61  NSYVFIVDMPGLKSGDIKVQVERDNVLVISGKRNREEE--KEGVKYVRMERRMGKFMKKF 120

Query: 121 PLPETADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
            LPE A+ +K+SA+ QDGVL+VTVEK PPPEPKK KTI+VQVA
Sbjct: 121 ALPEDANTDKISAICQDGVLTVTVEKLPPPEPKKPKTIQVQVA 160

[Close]

BLAST of MS008621 vs. ExPASy Swiss-Prot
Match: P29830 (17.6 kDa class II heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.6 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 5.1e-44
Identity = 88/147 (59.86%), Postives = 117/147 (79.59%), Query Frame = 0


Query: 14  LNVLHDLIDF-TDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMPGLKPD 73
           +++L D+++   D   +   + PSR Y+RDAKAMAATPADV+E+PN Y F +DMPG+K D
Sbjct: 9   ISILEDMLEVPEDHNNEKTRNNPSRVYMRDAKAMAATPADVIEHPNAYAFVVDMPGIKGD 68

Query: 74  LIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKVSAVYQ 133
            IKVQ+E +  LVVSGER+RE+++N+  KYV+MERR+GK+++KF LPE AD +K+SAV  
Sbjct: 69  EIKVQVENDNVLVVSGERQRENKENEGVKYVRMERRMGKFMRKFQLPENADLDKISAVCH 128

Query: 134 DGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           DGVL VTV+K PPPEPKK KTI+VQVA
Sbjct: 129 DGVLKVTVQKLPPPEPKKPKTIQVQVA 155

[Close]

BLAST of MS008621 vs. ExPASy Swiss-Prot
Match: Q01545 (18.8 kDa class II heat shock protein OS=Ipomoea nil OX=35883 GN=SHSP-2 PE=2 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 5.1e-44
Identity = 92/156 (58.97%), Postives = 117/156 (75.00%), Query Frame = 0


Query: 8   GFDPIFLNVLHDLIDFTDEPGQSGH---HAPSRAYVRDAKAMAATPADVVEYPNCYQFAI 67
           G +P  L+ + D++DF D+  ++G      P RAYVRDAKAMAATPADV EYPN Y F  
Sbjct: 12  GLEPQLLSTIQDMLDFADDHDRAGRAPPEQPIRAYVRDAKAMAATPADVKEYPNSYVFIA 71

Query: 68  DMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEG-KYVKMERRLGKYLKKFPLPETAD 127
           DMPG+K   IKVQ+E++  LVVSGER    +  K+G KY++MERR+GK+++KF LPE A+
Sbjct: 72  DMPGVKAAEIKVQVEDDNVLVVSGERTEREKDEKDGVKYLRMERRVGKFMRKFVLPENAN 131

Query: 128 AEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
            E ++AVYQDGVL VTVEK PPPEPKK KT+EV+VA
Sbjct: 132 VEAINAVYQDGVLQVTVEKLPPPEPKKPKTVEVKVA 167

[Close]

BLAST of MS008621 vs. ExPASy Swiss-Prot
Match: P19242 (17.1 kDa class II heat shock protein OS=Pisum sativum OX=3888 GN=HSP17.7 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.0e-43
Identity = 93/150 (62.00%), Postives = 117/150 (78.00%), Query Frame = 0


Query: 11  PIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMPGLK 70
           P+F N LH ++D TD+  +   +AP+R YVRDAKAMAATPADV E+PN Y F +DMPG+K
Sbjct: 6   PLF-NTLHHIMDLTDDTTEKNLNAPTRTYVRDAKAMAATPADVKEHPNSYVFMVDMPGVK 65

Query: 71  PDLIKVQIEENQQLVVSGERKRESEKNKEG-KYVKMERRLGKYLKKFPLPETADAEKVSA 130
              IKVQ+E+   L++SGERKRE E  KEG KY+KMERR+GK ++KF LPE A+ E +SA
Sbjct: 66  SGDIKVQVEDENVLLISGERKREEE--KEGVKYLKMERRIGKLMRKFVLPENANIEAISA 125

Query: 131 VYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           + QDGVL+VTV K PPPEPKK KTI+V+VA
Sbjct: 126 ISQDGVLTVTVNKLPPPEPKKPKTIQVKVA 152

[Close]

BLAST of MS008621 vs. ExPASy Swiss-Prot
Match: O81822 (17.7 kDa class II heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.7 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.3e-43
Identity = 87/149 (58.39%), Postives = 119/149 (79.87%), Query Frame = 0


Query: 11  PIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMPGLK 70
           PIF ++L D+++  +E  +   + PSRAY+RDAKAMAATPADV+E+P+ Y FA+DMPG+K
Sbjct: 9   PIF-SILEDMLEAPEEQTEKTRNNPSRAYMRDAKAMAATPADVIEHPDAYVFAVDMPGIK 68

Query: 71  PDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKVSAV 130
            D I+VQIE    LVVSG+R+R++++N+  K+V+MERR+GK+++KF LP+ AD EK+SA 
Sbjct: 69  GDEIQVQIENENVLVVSGKRQRDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKISAA 128

Query: 131 YQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
             DGVL VT+ K PPPEPKK KTI+VQVA
Sbjct: 129 CNDGVLKVTIPKLPPPEPKKPKTIQVQVA 156

[Close]

BLAST of MS008621 vs. ExPASy TrEMBL
Match: A0A6J1C0H4 (17.1 kDa class II heat shock protein-like OS=Momordica charantia OX=3673 GN=LOC111007218 PE=3 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 5.8e-83
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60
           METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY
Sbjct: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60

Query: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120
           QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE
Sbjct: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120

Query: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA
Sbjct: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 159

[Close]

BLAST of MS008621 vs. ExPASy TrEMBL
Match: A0A0A0L5D4 (SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G646350 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 7.2e-65
Identity = 128/159 (80.50%), Postives = 143/159 (89.94%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60
           ME  K+ GFDP+FLN LHDL+DF+DEPGQ  HHAPSRAY+RDAKAMAATPADVVEYPN Y
Sbjct: 1   MEVAKMPGFDPLFLNALHDLLDFSDEPGQGSHHAPSRAYLRDAKAMAATPADVVEYPNSY 60

Query: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120
           QF IDMPGL  D IKV++E+  QLVVSGERKRESEK KEGK+V+MERRLGKYLKKF LPE
Sbjct: 61  QFTIDMPGLTSDQIKVKVEDG-QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFDLPE 120

Query: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           TADA+KVSA Y+DGVLSVTVEK+PPPEPKKAK+IEV+VA
Sbjct: 121 TADADKVSAAYRDGVLSVTVEKKPPPEPKKAKSIEVRVA 158

[Close]

BLAST of MS008621 vs. ExPASy TrEMBL
Match: A0A5A7V858 (17.3 kDa class II heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001910 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.6e-64
Identity = 128/159 (80.50%), Postives = 143/159 (89.94%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60
           M+  K+ GFDP+FLN LHDL+DF+DEPGQS HHAPSRAY+RDAKAMAATPADVVEYPN Y
Sbjct: 1   MDVAKM-GFDPLFLNALHDLLDFSDEPGQSSHHAPSRAYLRDAKAMAATPADVVEYPNSY 60

Query: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120
           QF IDMPGLK D IKV++E+  QLVVSGERKRESEK KEGK+V+MERRLGKYLKKF LPE
Sbjct: 61  QFTIDMPGLKSDQIKVKVEDG-QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFELPE 120

Query: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           TAD +K+SA YQDGVLSVTVEK+PPPEPKKAKTIE+ VA
Sbjct: 121 TADTDKISAAYQDGVLSVTVEKKPPPEPKKAKTIEIHVA 157

[Close]

BLAST of MS008621 vs. ExPASy TrEMBL
Match: A0A1S3BUN1 (17.3 kDa class II heat shock protein-like OS=Cucumis melo OX=3656 GN=LOC103493687 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.6e-64
Identity = 128/159 (80.50%), Postives = 143/159 (89.94%), Query Frame = 0


Query: 1   METTKVGGFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCY 60
           M+  K+ GFDP+FLN LHDL+DF+DEPGQS HHAPSRAY+RDAKAMAATPADVVEYPN Y
Sbjct: 1   MDVAKM-GFDPLFLNALHDLLDFSDEPGQSSHHAPSRAYLRDAKAMAATPADVVEYPNSY 60

Query: 61  QFAIDMPGLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPE 120
           QF IDMPGLK D IKV++E+  QLVVSGERKRESEK KEGK+V+MERRLGKYLKKF LPE
Sbjct: 61  QFTIDMPGLKSDQIKVKVEDG-QLVVSGERKRESEKVKEGKFVRMERRLGKYLKKFELPE 120

Query: 121 TADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           TAD +K+SA YQDGVLSVTVEK+PPPEPKKAKTIE+ VA
Sbjct: 121 TADTDKISAAYQDGVLSVTVEKKPPPEPKKAKTIEIHVA 157

[Close]

BLAST of MS008621 vs. ExPASy TrEMBL
Match: A0A6J1J4H9 (17.1 kDa class II heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC111481248 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.7e-64
Identity = 130/151 (86.09%), Postives = 137/151 (90.73%), Query Frame = 0


Query: 8   GFDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMP 67
           G DPIFLN LHDL+DFTDEPGQS HH PSRAYVRDAKAMAATPADVVEYPN YQF+IDMP
Sbjct: 7   GLDPIFLNALHDLLDFTDEPGQSTHHGPSRAYVRDAKAMAATPADVVEYPNAYQFSIDMP 66

Query: 68  GLKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKV 127
           GLK D IKVQIE+N QLVV+GERKRESEK KEGKYV MERRLGKYLKKF LPE AD +KV
Sbjct: 67  GLKMDQIKVQIEDN-QLVVTGERKRESEKAKEGKYVTMERRLGKYLKKFVLPEAADVDKV 126

Query: 128 SAVYQDGVLSVTVEKRPPPEPKKAKTIEVQV 159
           SA YQDGVLSVTVEK+PPPEPKKAKTIEV+V
Sbjct: 127 SAAYQDGVLSVTVEKKPPPEPKKAKTIEVRV 156

[Close]

BLAST of MS008621 vs. TAIR 10
Match: AT5G12020.1 (17.6 kDa class II heat shock protein )

HSP 1 Score: 178.7 bits (452), Expect = 3.7e-45
Identity = 88/147 (59.86%), Postives = 117/147 (79.59%), Query Frame = 0


Query: 14  LNVLHDLIDF-TDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMPGLKPD 73
           +++L D+++   D   +   + PSR Y+RDAKAMAATPADV+E+PN Y F +DMPG+K D
Sbjct: 9   ISILEDMLEVPEDHNNEKTRNNPSRVYMRDAKAMAATPADVIEHPNAYAFVVDMPGIKGD 68

Query: 74  LIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKVSAVYQ 133
            IKVQ+E +  LVVSGER+RE+++N+  KYV+MERR+GK+++KF LPE AD +K+SAV  
Sbjct: 69  EIKVQVENDNVLVVSGERQRENKENEGVKYVRMERRMGKFMRKFQLPENADLDKISAVCH 128

Query: 134 DGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           DGVL VTV+K PPPEPKK KTI+VQVA
Sbjct: 129 DGVLKVTVQKLPPPEPKKPKTIQVQVA 155

[Close]

BLAST of MS008621 vs. TAIR 10
Match: AT5G12030.1 (heat shock protein 17.6A )

HSP 1 Score: 176.0 bits (445), Expect = 2.4e-44
Identity = 87/149 (58.39%), Postives = 119/149 (79.87%), Query Frame = 0


Query: 11  PIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMPGLK 70
           PIF ++L D+++  +E  +   + PSRAY+RDAKAMAATPADV+E+P+ Y FA+DMPG+K
Sbjct: 9   PIF-SILEDMLEAPEEQTEKTRNNPSRAYMRDAKAMAATPADVIEHPDAYVFAVDMPGIK 68

Query: 71  PDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKVSAV 130
            D I+VQIE    LVVSG+R+R++++N+  K+V+MERR+GK+++KF LP+ AD EK+SA 
Sbjct: 69  GDEIQVQIENENVLVVSGKRQRDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKISAA 128

Query: 131 YQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
             DGVL VT+ K PPPEPKK KTI+VQVA
Sbjct: 129 CNDGVLKVTIPKLPPPEPKKPKTIQVQVA 156

[Close]

BLAST of MS008621 vs. TAIR 10
Match: AT1G54050.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 92.8 bits (229), Expect = 2.6e-19
Identity = 52/114 (45.61%), Postives = 82/114 (71.93%), Query Frame = 0


Query: 50  PADVVEYPNCYQFAIDMPGLKPDLIKVQIEENQQLVV--SGERKRESEKNKEG-KYVKME 109
           P D++E P  Y F +D+PG+    I+V +EE + LV+  +G+RKR+ ++++EG KY+++E
Sbjct: 43  PIDILESPKEYIFYLDIPGISKSDIQVTVEEERTLVIKSNGKRKRDDDESEEGSKYIRLE 102

Query: 110 RRLGKYL-KKFPLPETADAEKVSAVYQDGVLSVTVEKRPPPEPKKAKTIEVQVA 160
           RRL + L KKF LPE AD   V+A YQ+GVL+V + K+ PP+P K KT+++ V+
Sbjct: 103 RRLAQNLVKKFRLPEDADMASVTAKYQEGVLTVVI-KKLPPQPPKPKTVQIAVS 155

[Close]

BLAST of MS008621 vs. TAIR 10
Match: AT1G53540.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-16
Identity = 54/148 (36.49%), Postives = 84/148 (56.76%), Query Frame = 0


Query: 9   FDPIFLNVLHDLIDFTDEPGQSGHHAPSRAYVRDAKAMAATPADVVEYPNCYQFAIDMPG 68
           FDP  L+V      F    G +  +AP+     D  A      D  E P  + F  D+PG
Sbjct: 16  FDPFSLDVFDPFEGFLTPSGLA--NAPA----MDVAAFTNAKVDWRETPEAHVFKADLPG 75

Query: 69  LKPDLIKVQIEENQQLVVSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKVS 128
           L+ + +KV++E+   L +SGER  E+E+ K  K+ ++ER  GK+ ++F LPE A  E++ 
Sbjct: 76  LRKEEVKVEVEDGNILQISGERSNENEE-KNDKWHRVERSSGKFTRRFRLPENAKMEEIK 135

Query: 129 AVYQDGVLSVTVEKRPPPEPKKAKTIEV 157
           A  ++GVLSVTV K P  +P + K+I++
Sbjct: 136 ASMENGVLSVTVPKVPEKKP-EVKSIDI 155

[Close]

BLAST of MS008621 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 84.0 bits (206), Expect = 1.2e-16
Identity = 51/134 (38.06%), Postives = 78/134 (58.21%), Query Frame = 0


Query: 31  GHHAPSRAY-----VRDAKAMAATPADVVEYPNCYQFAIDMPGLKPDLIKVQIEENQQLV 90
           G   PS A       RD  A      D  E P  + F  D+PGLK + +KV++E+   L 
Sbjct: 29  GFFTPSSALANASTARDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQ 88

Query: 91  VSGERKRESEKNKEGKYVKMERRLGKYLKKFPLPETADAEKVSAVYQDGVLSVTVEKRPP 150
           +SGER +E+E+ K  K+ ++ER  GK++++F LPE A  E+V A  ++GVL+V V K P 
Sbjct: 89  ISGERSKENEE-KNDKWHRVERASGKFMRRFRLPENAKMEEVKATMENGVLTVVVPKAPE 148

Query: 151 PEPKKAKTIEVQVA 160
            +P + K+I++  A
Sbjct: 149 KKP-QVKSIDISGA 160

The following BLAST results are available for this feature:

BLAST of MS008621 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135195.1	1.2e-82	100.00	17.1 kDa class II heat shock protein-like [Momordica charantia]	[more]
XP_004141335.2	1.5e-64	80.50	17.3 kDa class II heat shock protein [Cucumis sativus]	[more]
XP_008452768.1	3.3e-64	80.50	PREDICTED: 17.3 kDa class II heat shock protein-like [Cucumis melo] >KAA0064522....	[more]
XP_022982419.1	5.6e-64	86.09	17.1 kDa class II heat shock protein-like [Cucurbita maxima]	[more]
KAE8649916.1	2.8e-63	79.62	hypothetical protein Csa_012089 [Cucumis sativus]	[more]

BLAST of MS008621 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P46516	7.9e-45	60.74	17.9 kDa class II heat shock protein OS=Helianthus annuus OX=4232 GN=HSP17.9 PE=...	[more]
P29830	5.1e-44	59.86	17.6 kDa class II heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.6 ...	[more]
Q01545	5.1e-44	58.97	18.8 kDa class II heat shock protein OS=Ipomoea nil OX=35883 GN=SHSP-2 PE=2 SV=1	[more]
P19242	2.0e-43	62.00	17.1 kDa class II heat shock protein OS=Pisum sativum OX=3888 GN=HSP17.7 PE=1 SV...	[more]
O81822	3.3e-43	58.39	17.7 kDa class II heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.7 ...	[more]

BLAST of MS008621 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C0H4	5.8e-83	100.00	17.1 kDa class II heat shock protein-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A0A0L5D4	7.2e-65	80.50	SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G646350 PE=3 S...	[more]
A0A5A7V858	1.6e-64	80.50	17.3 kDa class II heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BUN1	1.6e-64	80.50	17.3 kDa class II heat shock protein-like OS=Cucumis melo OX=3656 GN=LOC10349368...	[more]
A0A6J1J4H9	2.7e-64	86.09	17.1 kDa class II heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of MS008621 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G12020.1	3.7e-45	59.86	17.6 kDa class II heat shock protein	[more]
AT5G12030.1	2.4e-44	58.39	heat shock protein 17.6A	[more]
AT1G54050.1	2.6e-19	45.61	HSP20-like chaperones superfamily protein	[more]
AT1G53540.1	1.2e-16	36.49	HSP20-like chaperones superfamily protein	[more]
AT5G59720.1	1.2e-16	38.06	heat shock protein 18.2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 9..158 e-value: 3.8E-34 score: 119.5
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 34..152
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 52..157 e-value: 1.4E-26 score: 92.6
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 42..159 score: 27.614367
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 140..159
None	No IPR available	PANTHER	PTHR11527:SF273	18.8 KDA CLASS II HEAT SHOCK PROTEIN-LIKE	coord: 9..159
None	No IPR available	CDD	cd06464	ACD_sHsps-like	coord: 52..142 e-value: 3.4138E-28 score: 98.01
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 9..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008621.1	MS008621.1	mRNA