MS008547 (gene) Bitter gourd (TR) v1

BLAST of MS008547 vs. NCBI nr
Match: XP_022135512.1 (brassinosteroid-related acyltransferase 1 [Momordica charantia])

HSP 1 Score: 900.2 bits (2325), Expect = 7.3e-258
Identity = 447/454 (98.46%), Postives = 451/454 (99.34%), Query Frame = 0

Query: 1   ETHSQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFT 60
           ETHSQVSVTKI+SVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFT
Sbjct: 6   ETHSQVSVTKIISVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFT 65

Query: 61  KLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNE 120
           KLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRG ILVQAMTNVEISGLGDLSKYNE
Sbjct: 66  KLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGVILVQAMTNVEISGLGDLSKYNE 125

Query: 121 FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS 180
           FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS
Sbjct: 126 FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS 185

Query: 181 AIFKQRMEHQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEM 240
           AIFKQRMEHQVELQRPVHQRGSLLDMAKF NPRSSASAAVAIEHLYGLIMQAGRENNGEM
Sbjct: 186 AIFKQRMEHQVELQRPVHQRGSLLDMAKFGNPRSSASAAVAIEHLYGLIMQAGRENNGEM 245

Query: 241 GKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKE 300
           GKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKE
Sbjct: 246 GKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKE 305

Query: 301 KIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSRVN 360
           K+VCLQFAVDARNRLLPPLPKGFSGNAFVLASV LTAKQLEE+SHRTIVEMIQNAKSRVN
Sbjct: 306 KMVCLQFAVDARNRLLPPLPKGFSGNAFVLASVTLTAKQLEEKSHRTIVEMIQNAKSRVN 365

Query: 361 NDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAY 420
           NDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAY
Sbjct: 366 NDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAY 425

Query: 421 FLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           FLQNPADSQ+IDVRVTLLSHTLDTFSSYFMTSMQ
Sbjct: 426 FLQNPADSQSIDVRVTLLSHTLDTFSSYFMTSMQ 459

BLAST of MS008547 vs. NCBI nr
Match: XP_038898484.1 (brassinosteroid-related acyltransferase 1 [Benincasa hispida])

HSP 1 Score: 687.2 bits (1772), Expect = 9.7e-194
Identity = 350/462 (75.76%), Postives = 396/462 (85.71%), Query Frame = 0

Query: 1   ETHSQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFT 60
           E+HSQVS+TK ++VFPKTLNPPKLLELSNLDRQCPLHMYL+FFYNPS  YS+SSLDLVFT
Sbjct: 6   ESHSQVSLTKFLTVFPKTLNPPKLLELSNLDRQCPLHMYLIFFYNPSSVYSNSSLDLVFT 65

Query: 61  KLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNE 120
           KLK GLE+TL++W  AAGRL+ + TDGKLNLRC+NRGAILVQA+TNV++S LGDLS YNE
Sbjct: 66  KLKIGLEQTLSIWFPAAGRLDRNSTDGKLNLRCDNRGAILVQAITNVKLSELGDLSNYNE 125

Query: 121 FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS 180
           F E LV+KPVLNGNFSEFPLVVAQ+TRFGCGGY +GTGISHSLFDGPAA+DFL+AWAA S
Sbjct: 126 FFENLVHKPVLNGNFSEFPLVVAQVTRFGCGGYAIGTGISHSLFDGPAAYDFLSAWAANS 185

Query: 181 AIFKQRMEHQVELQR---PVHQRGSLL--DMAKFRNPRSSASAAVAIEHLYGLIMQAGRE 240
           AIFK        L R   P+H+RG LL   +A   +   + + AVAI+HLY LIMQ+   
Sbjct: 186 AIFKDHQYGGELLHRIISPMHERGRLLTGSVANRCSRSGTGTVAVAIDHLYKLIMQSAMG 245

Query: 241 NNGEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGAN---AAFHCSSFDVIAAHLWKART 300
           N  E+GK    EY C TFRV+R MI+RLK NA LG N   AAF CSSFDVIAAHLWKART
Sbjct: 246 NEEEVGKK---EYVCKTFRVNRGMIERLKKNA-LGENGVAAAFACSSFDVIAAHLWKART 305

Query: 301 KALSVNKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMI 360
           KALSV+KEK+VCLQFAVDARN+L+PPLPKGFSGNAFVLASVALTAKQLEEES++TI+EMI
Sbjct: 306 KALSVSKEKMVCLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLEEESYKTIIEMI 365

Query: 361 QNAKSRVNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLK 420
           +NAKS+V+NDY++AY+ GLEGPQASLPPLKELTVVSDWTRMPFHKI+FLHGQA YASPLK
Sbjct: 366 RNAKSQVDNDYINAYQHGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQATYASPLK 425

Query: 421 PPILEVAYFLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           PPILEVAYFLQNPADSQAIDV V LL HT D FSSYF+T MQ
Sbjct: 426 PPILEVAYFLQNPADSQAIDVHVPLLEHTFDAFSSYFLTRMQ 463

BLAST of MS008547 vs. NCBI nr
Match: XP_022941116.1 (brassinosteroid-related acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 669.8 bits (1727), Expect = 1.6e-188
Identity = 348/458 (75.98%), Postives = 393/458 (85.81%), Query Frame = 0

Query: 4   SQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLK 63
           S VSVTK ++VFPKTLN PKLLELSNLDRQCPLH+YL+FFY PS   SSSS D VFTKLK
Sbjct: 4   SHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSFDSVFTKLK 63

Query: 64  AGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNEFSE 123
            GLEETL+VW  AAGRLEP+P +GKLNLRCNNRGAILVQ+ TNVEIS LGDLS YNEF E
Sbjct: 64  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNVEISKLGDLSNYNEFFE 123

Query: 124 KLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIF 183
           KLVYKPVLNGNFSEFPLVVAQ+TRFGCGGY++GTGISHSLFDGPAA++FL+AWAAKSAIF
Sbjct: 124 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 183

Query: 184 KQRME-HQVELQRPVHQRGSLL---DMAKFRNPRS-SASAAVAIEHLYGLIMQA--GREN 243
           K + E + VEL  PVH+RG LL   D  +    R+   S AVAI+HLY LIMQ+  G+E 
Sbjct: 184 KDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMPVSGAVAIDHLYRLIMQSMTGKE- 243

Query: 244 NGEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALS 303
             EMGK    EY   TFR+SR MI+RLK NA +G N  F CSSFDVIAAHLWKARTKA+S
Sbjct: 244 --EMGKK---EYVYRTFRLSRGMIERLKKNA-MGENGGFACSSFDVIAAHLWKARTKAMS 303

Query: 304 VNKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAK 363
           V+KEK+VCLQFAV+ARN+L+PPLPKGFSGNAFVLASVALTA+QLE+ES++TIV+MI+NAK
Sbjct: 304 VSKEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIRNAK 363

Query: 364 SRVNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPIL 423
           SRV+NDY++AYK GLEGPQASLPPLKELTVVSDWTRMPFHKI+FLHGQA YASPLKPPIL
Sbjct: 364 SRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPIL 423

Query: 424 EVAYFLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           +VAYFLQ+P DSQAIDVRV LL  TL+ FSSYF+T MQ
Sbjct: 424 DVAYFLQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of MS008547 vs. NCBI nr
Match: XP_022981142.1 (brassinosteroid-related acyltransferase 1 [Cucurbita maxima])

HSP 1 Score: 669.5 bits (1726), Expect = 2.1e-188
Identity = 347/460 (75.43%), Postives = 393/460 (85.43%), Query Frame = 0

Query: 4   SQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLK 63
           S VSVTK ++VFPKTLN PKLLELSNLDRQCPLH+YL+FFY PS   SSSSLD +FT LK
Sbjct: 4   SHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSLDSIFTNLK 63

Query: 64  AGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNEFSE 123
            GLEETL+VW  AAGRLEP+P +GKLNLRCNNRGAILVQ+ TN+EIS LGDLS YNEF E
Sbjct: 64  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 123

Query: 124 KLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIF 183
           KLVYKPVLNGNFSEFPLVVAQ+TRFGCGGY++GTGISHSLFDGPAA++FL+AWAAKSAIF
Sbjct: 124 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 183

Query: 184 KQRME-HQVELQRPVHQRGSLL---DMAKFRNPRS---SASAAVAIEHLYGLIMQA--GR 243
           K + E + VEL  PVH+RG LL   D  +    R+   S S AVAI+HLY LIMQ+  G+
Sbjct: 184 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 243

Query: 244 ENNGEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKA 303
           E   EMGK    EY   TFR+SR MI+RLK NA +G N  F CSSFDVIAAHLWKARTKA
Sbjct: 244 E---EMGKK---EYVYRTFRLSRGMIERLKKNA-MGENGGFACSSFDVIAAHLWKARTKA 303

Query: 304 LSVNKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQN 363
           +SV+ EK+VCLQFAV+ARN+L+PPLPKGFSGNAFVLASVALTA+QLE+ES++TIVEMI+N
Sbjct: 304 MSVSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRN 363

Query: 364 AKSRVNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPP 423
           AKSRV+NDY++AYK GLEGPQASLPPLKELTVVSDWTRMPFHKI+FLHGQA YASPLKPP
Sbjct: 364 AKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPP 423

Query: 424 ILEVAYFLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           IL+VAYFLQ+P DSQAIDVRV LL  TL+ FSSYF+T MQ
Sbjct: 424 ILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 453

BLAST of MS008547 vs. NCBI nr
Match: KAG6608286.1 (Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037638.1 Brassinosteroid-related acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 668.3 bits (1723), Expect = 4.7e-188
Identity = 347/458 (75.76%), Postives = 393/458 (85.81%), Query Frame = 0

Query: 4   SQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLK 63
           S VSVTK ++VFPKTLN PKLLELSNLDRQCPLH+YL+FFY PS   SSSS D VFTKLK
Sbjct: 4   SHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSFDSVFTKLK 63

Query: 64  AGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNEFSE 123
            GLEETL+VW  AAGRLEP+P +GKLNLRCNNRGAILV++ TNVEIS LGDLS YNEF E
Sbjct: 64  MGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVRSFTNVEISELGDLSNYNEFFE 123

Query: 124 KLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIF 183
           KLVYKPVLNGNFSEFPLVVAQ+TRFGCGGY++GTGISHSLFDGPAA++FL+AWAAKSAIF
Sbjct: 124 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 183

Query: 184 KQRME-HQVELQRPVHQRGSLL---DMAKFRNPRS-SASAAVAIEHLYGLIMQA--GREN 243
           K + E + VEL  PVH+RG LL   D  +    R+   S AVAI+HLY LIMQ+  G+E 
Sbjct: 184 KDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMPVSGAVAIDHLYRLIMQSMTGKE- 243

Query: 244 NGEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALS 303
             EMGK    EY   TFR+SR MI+RLK NA +G N  F CSSFDVIAAHLWKARTKA+S
Sbjct: 244 --EMGKK---EYVYRTFRLSRGMIERLKKNA-MGENGGFACSSFDVIAAHLWKARTKAMS 303

Query: 304 VNKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAK 363
           V+KEK+VCLQFAV+ARN+L+PPLPKGFSGNAFVLASVALTA+QLE+ES++TIV+MI+NAK
Sbjct: 304 VSKEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIRNAK 363

Query: 364 SRVNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPIL 423
           SRV+NDY++AYK GLEGPQASLPPLKELTVVSDWTRMPFHKI+FLHGQA YASPLKPPIL
Sbjct: 364 SRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPIL 423

Query: 424 EVAYFLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           +VAYFLQ+P DSQAIDVRV LL  TL+ FSSYF+T MQ
Sbjct: 424 DVAYFLQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of MS008547 vs. ExPASy Swiss-Prot
Match: Q9SZ58 (Brassinosteroid-related acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=BAT1 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.2e-103
Identity = 214/462 (46.32%), Postives = 308/462 (66.67%), Query Frame = 0

Query: 4   SQVSVTKIVSVFPKTLN--PPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTK 63
           +++ + + ++V+P+  N    KL+ LSNLDRQCPL MY VFFY  +   ++   D VF+ 
Sbjct: 7   TRIDIIQKLNVYPRFQNHDKKKLITLSNLDRQCPLLMYSVFFYKNT---TTRDFDSVFSN 66

Query: 64  LKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAM-TNVEISGLGDLSKYNE 123
           LK GLEET++VW  AAGRL       KLN+RCN+ GA++V+A+ T V++S LGDL++YNE
Sbjct: 67  LKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLTQYNE 126

Query: 124 FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS 183
           F E LVYKP L+G+FS  PLVVAQ+TRF CGGY++G G SHSLFDG +A++F+ AWA+ S
Sbjct: 127 FYENLVYKPSLDGDFSVMPLVVAQVTRFACGGYSIGIGTSHSLFDGISAYEFIHAWASNS 186

Query: 184 AIFKQ---RMEHQVE--LQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQA--- 243
            I  +   ++ ++ E  + +PVH R +LL            + A AI HLY LI QA   
Sbjct: 187 HIHNKSNSKITNKKEDVVIKPVHDRRNLL----VNRDAVRETNAAAICHLYQLIKQAMMT 246

Query: 244 GRENNGEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKART 303
            +E N  + +     +   TF ++ + I+ +K  +  G    F CSSF+ +AAHLWKART
Sbjct: 247 YQEQNRNL-ELPDSGFVIKTFELNGDAIESMKKKSLEG----FMCSSFEFLAAHLWKART 306

Query: 304 KALSVNKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQ-LEEESHRTIVEM 363
           +AL + ++ +VCLQFAVD R R   PLP+GFSGNA+VLASVA TA++ LEE +  +IV  
Sbjct: 307 RALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEELTLESIVNK 366

Query: 364 IQNAKSRVNNDYVHAYKLGLEGP-QASLPPLKELTVVSDWTRMPFHKIDFLHG--QAAYA 423
           I+ AK  ++  Y+++Y   L G    +LPPLKELT++SDWT+MPFH + F +G   A Y 
Sbjct: 367 IREAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGNGGEPADYM 426

Query: 424 SPLKPPILEVAYFLQNPADSQAIDVRVTLLSHTLDTFSSYFM 451
           +PL PP+ +VAYF++NP D++ + VR+ L    ++ FS++F+
Sbjct: 427 APLCPPVPQVAYFMKNPKDAKGVLVRIGLDPRDVNGFSNHFL 456

BLAST of MS008547 vs. ExPASy Swiss-Prot
Match: Q94CD1 (Omega-hydroxypalmitate O-feruloyl transferase OS=Arabidopsis thaliana OX=3702 GN=HHT1 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.6e-42
Identity = 127/451 (28.16%), Postives = 220/451 (48.78%), Query Frame = 0

Query: 3   HSQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKL 62
           H +V   +   V P++     L  LSNLD+   + +  ++ +      +  ++ ++    
Sbjct: 30  HLEVHQKEPALVKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQVI---- 89

Query: 63  KAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYN-EF 122
           K  L + L  +   AGRL  SP +GKL + C   G + V+A  N ++  +GD++K + E 
Sbjct: 90  KKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPET 149

Query: 123 SEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSA 182
             KLVY  V   N  E P V AQ+T+F CGG+ +G  ++H +FDG  A +F+ +W     
Sbjct: 150 LGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWG---- 209

Query: 183 IFKQRMEHQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEMG 242
               ++   + L  P     ++L+    RNP         IE+L+    +   ++N    
Sbjct: 210 ----QVARGLPLTTPPFSDRTILNA---RNPPK-------IENLHQEFEEIEDKSNINSL 269

Query: 243 KNKKDEYFCTTFRVSREMIQRLKNNANLGANAAF--HCSSFDVIAAHLWKARTKALSVNK 302
             K+   +  +F    E I++LK  A   + +     C+SF+ ++A +W+ARTK+L +  
Sbjct: 270 YTKEPTLY-RSFCFDPEKIKKLKLQATENSESLLGNSCTSFEALSAFVWRARTKSLKMLS 329

Query: 303 EKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSRV 362
           ++   L FAVD R +  P LPKG+ GN  VL +    A +L E+     V +++ A   V
Sbjct: 330 DQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAIKMV 389

Query: 363 NNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVA 422
            + Y+ +     E  +A  P L    +++ W+R+ FH  DF  G+   + P+  P  EV 
Sbjct: 390 TDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEKEVT 449

Query: 423 YFLQNPADSQAIDVRVTLLSHTLDTFSSYFM 451
            FL +    ++I+V + L +  +D F   F+
Sbjct: 450 LFLSHGEQRRSINVLLGLPATAMDVFQEQFL 455

BLAST of MS008547 vs. ExPASy Swiss-Prot
Match: Q9FFQ7 (Fatty alcohol:caffeoyl-CoA acyltransferase OS=Arabidopsis thaliana OX=3702 GN=FACT PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.4e-38
Identity = 127/438 (29.00%), Postives = 204/438 (46.58%), Query Frame = 0

Query: 12  VSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLKAGLEETLA 71
           V V P +  P  L  LSNLD+   + +   +++  +   +  S +++    K  L E L 
Sbjct: 15  VLVSPASETPKGLHYLSNLDQNIAIIVKTFYYFKSNSRSNEESYEVI----KKSLSEVLV 74

Query: 72  VWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGD-LSKYN--EFSEKLVYK 131
            +  AAGRL  SP +GK+ + C   G ++V+A  N  I  +   +S+ +  E  EKLVY 
Sbjct: 75  HYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQPETLEKLVYD 134

Query: 132 PVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIFKQRME 191
                N  E P VV Q+T F CGG+ +G G++H++FDG AA +FL +WA  +        
Sbjct: 135 VPGARNILEIPPVVVQVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAETA-------- 194

Query: 192 HQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEMGKNKKDEY 251
             + L  P     +LL       PR+             L      E+    GK   DE 
Sbjct: 195 RGLPLSVPPFLDRTLL------RPRTPPKIEFPHNEFEDL------EDISGTGKLYSDEK 254

Query: 252 FC-TTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKEKIVCLQF 311
               +F    E ++RLK        A    ++F  +   LW+AR +AL +  ++ + L F
Sbjct: 255 LVYKSFLFGPEKLERLK------IMAETRSTTFQTLTGFLWRARCQALGLKPDQRIKLLF 314

Query: 312 AVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSRVNNDYVHAY 371
           A D R+R +P LPKG+SGN  V      TA ++        V +++ A   VN+ ++ + 
Sbjct: 315 AADGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEMVNDGFMRSA 374

Query: 372 KLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAYFLQNPAD 431
               E  +A  P L    +++ W ++ FH  DF  G+   + P+  P  EV  FL   +D
Sbjct: 375 IDYFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEVILFLPCGSD 420

Query: 432 SQAIDVRVTLLSHTLDTF 446
           +++I+V + L    +  F
Sbjct: 435 TKSINVLLGLPGSAMKVF 420

BLAST of MS008547 vs. ExPASy Swiss-Prot
Match: Q8S9G6 (Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus wallichiana var. chinensis OX=29808 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.2e-34
Identity = 111/415 (26.75%), Postives = 196/415 (47.23%), Query Frame = 0

Query: 1   ETHSQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLH---MYLVFFYNPSPAYSSSSLDL 60
           +T   V++ + V V P    P   L+LS++D    +       +  YN SP+ +  S D 
Sbjct: 3   KTDLHVNLNEKVMVGPSLPLPKTTLQLSSIDNLPGVRGSIFNALLIYNASPSPTMVSADP 62

Query: 61  VFTKLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSK 120
               ++  L + L  +P  AGRL  +  +G L + C   GA+ ++AM + E+S LGD   
Sbjct: 63  A-KLIREALAKILVYYPPFAGRLRET-ENGDLEVECTGEGAMFLEAMADNELSVLGDFDD 122

Query: 121 YNEFSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWA 180
            N   ++L++   L+ NF + PL+V Q+TRF CGG+ VG    H + DG  A  FL   A
Sbjct: 123 SNPSFQQLLFSLPLDTNFKDLPLLVVQVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLA 182

Query: 181 AKSAIFKQRMEHQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENN 240
             +     R E ++ L+ P+  R    ++ K  +P+        ++  +   ++A     
Sbjct: 183 EMA-----RGEVKLSLE-PIWNR----ELVKLDDPK-------YLQFFHFEFLRA----- 242

Query: 241 GEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSV 300
                +  ++   T F +  E I  +K   ++       CSSF+V +A  W ART+A  +
Sbjct: 243 ----PSIVEKIVQTYFIIDFETINYIKQ--SVMEECKEFCSSFEVASAMTWIARTRAFQI 302

Query: 301 NKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKS 360
            + + V + F +D RN   PPLP G+ GN+   A      + L   S    + +I+ +K 
Sbjct: 303 PESEYVKILFGMDMRNSFNPPLPSGYYGNSIGTACAVDNVQDLLSGSLLRAIMIIKKSKV 362

Query: 361 RVNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLK 413
            +N+++     +       ++   + +   +DW+R+ F ++DF  G A   SP++
Sbjct: 363 SLNDNFKSRAVVKPSELDVNMNH-ENVVAFADWSRLGFDEVDFGWGNAVSVSPVQ 386

BLAST of MS008547 vs. ExPASy Swiss-Prot
Match: Q9M6F0 (Taxadien-5-alpha-ol O-acetyltransferase OS=Taxus cuspidata OX=99806 GN=TAT PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 5.2e-33
Identity = 110/415 (26.51%), Postives = 195/415 (46.99%), Query Frame = 0

Query: 1   ETHSQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLH---MYLVFFYNPSPAYSSSSLDL 60
           +T   V++ + V V P    P   L+LS++D    +       +  YN SP+ +  S D 
Sbjct: 3   KTDLHVNLIEKVMVGPSPPLPKTTLQLSSIDNLPGVRGSIFNALLIYNASPSPTMISADP 62

Query: 61  VFTKLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSK 120
               ++  L + L  +P  AGRL  +  +G L + C   GA+ ++AM + E+S LGD   
Sbjct: 63  A-KPIREALAKILVYYPPFAGRLRET-ENGDLEVECTGEGAMFLEAMADNELSVLGDFDD 122

Query: 121 YNEFSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWA 180
            N   ++L++   L+ NF +  L+V Q+TRF CGG+ VG    H + DG  A  FL   A
Sbjct: 123 SNPSFQQLLFSLPLDTNFKDLSLLVVQVTRFTCGGFVVGVSFHHGVCDGRGAAQFLKGLA 182

Query: 181 AKSAIFKQRMEHQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENN 240
             +     R E ++ L+ P+  R    ++ K  +P+        ++  +   ++A     
Sbjct: 183 EMA-----RGEVKLSLE-PIWNR----ELVKLDDPK-------YLQFFHFEFLRA----- 242

Query: 241 GEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSV 300
                +  ++   T F +  E I  +K   ++       CSSF+V +A  W ART+A  +
Sbjct: 243 ----PSIVEKIVQTYFIIDFETINYIKQ--SVMEECKEFCSSFEVASAMTWIARTRAFQI 302

Query: 301 NKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKS 360
            + + V + F +D RN   PPLP G+ GN+   A      + L   S    + +I+ +K 
Sbjct: 303 PESEYVKILFGMDMRNSFNPPLPSGYYGNSIGTACAVDNVQDLLSGSLLRAIMIIKKSKV 362

Query: 361 RVNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLK 413
            +N+++     +       ++   + +   +DW+R+ F ++DF  G A   SP++
Sbjct: 363 SLNDNFKSRAVVKPSELDVNMNH-ENVVAFADWSRLGFDEVDFGWGNAVSVSPVQ 386

BLAST of MS008547 vs. ExPASy TrEMBL
Match: A0A6J1C2X0 (brassinosteroid-related acyltransferase 1 OS=Momordica charantia OX=3673 GN=LOC111007449 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 3.5e-258
Identity = 447/454 (98.46%), Postives = 451/454 (99.34%), Query Frame = 0

Query: 1   ETHSQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFT 60
           ETHSQVSVTKI+SVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFT
Sbjct: 6   ETHSQVSVTKIISVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFT 65

Query: 61  KLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNE 120
           KLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRG ILVQAMTNVEISGLGDLSKYNE
Sbjct: 66  KLKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGVILVQAMTNVEISGLGDLSKYNE 125

Query: 121 FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS 180
           FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS
Sbjct: 126 FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS 185

Query: 181 AIFKQRMEHQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEM 240
           AIFKQRMEHQVELQRPVHQRGSLLDMAKF NPRSSASAAVAIEHLYGLIMQAGRENNGEM
Sbjct: 186 AIFKQRMEHQVELQRPVHQRGSLLDMAKFGNPRSSASAAVAIEHLYGLIMQAGRENNGEM 245

Query: 241 GKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKE 300
           GKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKE
Sbjct: 246 GKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKE 305

Query: 301 KIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSRVN 360
           K+VCLQFAVDARNRLLPPLPKGFSGNAFVLASV LTAKQLEE+SHRTIVEMIQNAKSRVN
Sbjct: 306 KMVCLQFAVDARNRLLPPLPKGFSGNAFVLASVTLTAKQLEEKSHRTIVEMIQNAKSRVN 365

Query: 361 NDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAY 420
           NDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAY
Sbjct: 366 NDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAY 425

Query: 421 FLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           FLQNPADSQ+IDVRVTLLSHTLDTFSSYFMTSMQ
Sbjct: 426 FLQNPADSQSIDVRVTLLSHTLDTFSSYFMTSMQ 459

BLAST of MS008547 vs. ExPASy TrEMBL
Match: A0A6J1FK90 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111446510 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 7.8e-189
Identity = 348/458 (75.98%), Postives = 393/458 (85.81%), Query Frame = 0

Query: 4   SQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLK 63
           S VSVTK ++VFPKTLN PKLLELSNLDRQCPLH+YL+FFY PS   SSSS D VFTKLK
Sbjct: 4   SHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSFDSVFTKLK 63

Query: 64  AGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNEFSE 123
            GLEETL+VW  AAGRLEP+P +GKLNLRCNNRGAILVQ+ TNVEIS LGDLS YNEF E
Sbjct: 64  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNVEISKLGDLSNYNEFFE 123

Query: 124 KLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIF 183
           KLVYKPVLNGNFSEFPLVVAQ+TRFGCGGY++GTGISHSLFDGPAA++FL+AWAAKSAIF
Sbjct: 124 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 183

Query: 184 KQRME-HQVELQRPVHQRGSLL---DMAKFRNPRS-SASAAVAIEHLYGLIMQA--GREN 243
           K + E + VEL  PVH+RG LL   D  +    R+   S AVAI+HLY LIMQ+  G+E 
Sbjct: 184 KDKKEPYGVELHTPVHERGRLLMGYDAKRSLEKRAMPVSGAVAIDHLYRLIMQSMTGKE- 243

Query: 244 NGEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALS 303
             EMGK    EY   TFR+SR MI+RLK NA +G N  F CSSFDVIAAHLWKARTKA+S
Sbjct: 244 --EMGKK---EYVYRTFRLSRGMIERLKKNA-MGENGGFACSSFDVIAAHLWKARTKAMS 303

Query: 304 VNKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAK 363
           V+KEK+VCLQFAV+ARN+L+PPLPKGFSGNAFVLASVALTA+QLE+ES++TIV+MI+NAK
Sbjct: 304 VSKEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVDMIRNAK 363

Query: 364 SRVNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPIL 423
           SRV+NDY++AYK GLEGPQASLPPLKELTVVSDWTRMPFHKI+FLHGQA YASPLKPPIL
Sbjct: 364 SRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPPIL 423

Query: 424 EVAYFLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           +VAYFLQ+P DSQAIDVRV LL  TL+ FSSYF+T MQ
Sbjct: 424 DVAYFLQSPIDSQAIDVRVPLLGGTLEAFSSYFLTGMQ 451

BLAST of MS008547 vs. ExPASy TrEMBL
Match: A0A6J1IT61 (brassinosteroid-related acyltransferase 1 OS=Cucurbita maxima OX=3661 GN=LOC111480377 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 1.0e-188
Identity = 347/460 (75.43%), Postives = 393/460 (85.43%), Query Frame = 0

Query: 4   SQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLK 63
           S VSVTK ++VFPKTLN PKLLELSNLDRQCPLH+YL+FFY PS   SSSSLD +FT LK
Sbjct: 4   SHVSVTKTLTVFPKTLNSPKLLELSNLDRQCPLHVYLIFFYKPS---SSSSLDSIFTNLK 63

Query: 64  AGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNEFSE 123
            GLEETL+VW  AAGRLEP+P +GKLNLRCNNRGAILVQ+ TN+EIS LGDLS YNEF E
Sbjct: 64  IGLEETLSVWSPAAGRLEPNPANGKLNLRCNNRGAILVQSFTNIEISELGDLSNYNEFFE 123

Query: 124 KLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIF 183
           KLVYKPVLNGNFSEFPLVVAQ+TRFGCGGY++GTGISHSLFDGPAA++FL+AWAAKSAIF
Sbjct: 124 KLVYKPVLNGNFSEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWAAKSAIF 183

Query: 184 KQRME-HQVELQRPVHQRGSLL---DMAKFRNPRS---SASAAVAIEHLYGLIMQA--GR 243
           K + E + VEL  PVH+RG LL   D  +    R+   S S AVAI+HLY LIMQ+  G+
Sbjct: 184 KDKKEPYGVELHTPVHERGRLLMDYDAKRSSEKRAMPVSVSGAVAIDHLYRLIMQSVTGK 243

Query: 244 ENNGEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKA 303
           E   EMGK    EY   TFR+SR MI+RLK NA +G N  F CSSFDVIAAHLWKARTKA
Sbjct: 244 E---EMGKK---EYVYRTFRLSRGMIERLKKNA-MGENGGFACSSFDVIAAHLWKARTKA 303

Query: 304 LSVNKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQN 363
           +SV+ EK+VCLQFAV+ARN+L+PPLPKGFSGNAFVLASVALTA+QLE+ES++TIVEMI+N
Sbjct: 304 MSVSNEKMVCLQFAVNARNKLMPPLPKGFSGNAFVLASVALTARQLEDESYKTIVEMIRN 363

Query: 364 AKSRVNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPP 423
           AKSRV+NDY++AYK GLEGPQASLPPLKELTVVSDWTRMPFHKI+FLHGQA YASPLKPP
Sbjct: 364 AKSRVDNDYINAYKQGLEGPQASLPPLKELTVVSDWTRMPFHKIEFLHGQALYASPLKPP 423

Query: 424 ILEVAYFLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           IL+VAYFLQ+P DSQAIDVRV LL  TL+ FSSYF+T MQ
Sbjct: 424 ILDVAYFLQSPLDSQAIDVRVPLLEGTLEAFSSYFLTGMQ 453

BLAST of MS008547 vs. ExPASy TrEMBL
Match: A0A0A0L0F4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652090 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 1.1e-187
Identity = 333/451 (73.84%), Postives = 384/451 (85.14%), Query Frame = 0

Query: 5   QVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLKA 64
           QVS+TK ++VFPKT NP K +ELSNLDRQCPLHMYL+FFYNPS  Y++SS DLVF KLK 
Sbjct: 5   QVSLTKSLTVFPKTPNPTKFVELSNLDRQCPLHMYLIFFYNPSSIYTNSSPDLVFNKLKI 64

Query: 65  GLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNEFSEK 124
           GLEETL++W  AAGRL+ +PTDGKLNLRC+NRGAILVQA+TNV++S LGDLS YNEF EK
Sbjct: 65  GLEETLSIWSPAAGRLDRNPTDGKLNLRCDNRGAILVQAITNVKMSELGDLSNYNEFFEK 124

Query: 125 LVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIFK 184
           LV+KP+LNGNFSEFPLVVAQ+TRFGCGGY +GTGISHSLFDGPAA+DFL+AWAA SAIFK
Sbjct: 125 LVHKPILNGNFSEFPLVVAQVTRFGCGGYAIGTGISHSLFDGPAAYDFLSAWAANSAIFK 184

Query: 185 QRMEHQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEMGKNK 244
               ++++   P+H+RG LL          + + A AI+HLY LIMQ+    N E+G+  
Sbjct: 185 D--NNKMQTITPIHERGRLLKGTVANRCSRTVAVAAAIDHLYKLIMQSAM-GNEEVGRK- 244

Query: 245 KDEYFCTTFRVSREMIQRLKNNANLGAN-AAFHCSSFDVIAAHLWKARTKALSVNKEKIV 304
             EY C TFRVS+EMI+RLK NA    N AAF CSSFD+IAAHLWKARTKALSV+KEK+V
Sbjct: 245 --EYVCRTFRVSKEMIERLKMNALGETNGAAFSCSSFDLIAAHLWKARTKALSVSKEKMV 304

Query: 305 CLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSRVNNDY 364
           CLQFAVDARN+L+PPLPKGFSGNAFVLASVALTAKQL+EES++TI+EMIQNAKSRV+NDY
Sbjct: 305 CLQFAVDARNKLMPPLPKGFSGNAFVLASVALTAKQLDEESYKTIIEMIQNAKSRVDNDY 364

Query: 365 VHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAYFLQ 424
           ++AY+ GLEGPQASLPPLKELTVVSDWTRMPFHKI FLHG+AAYASPLKPP+LEVAYFLQ
Sbjct: 365 INAYQQGLEGPQASLPPLKELTVVSDWTRMPFHKIQFLHGEAAYASPLKPPVLEVAYFLQ 424

Query: 425 NPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           NP D QAIDV V LL HT D FSSYF+T MQ
Sbjct: 425 NPTDGQAIDVHVPLLEHTFDAFSSYFLTRMQ 449

BLAST of MS008547 vs. ExPASy TrEMBL
Match: A0A6J1IG59 (brassinosteroid-related acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111476630 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 2.1e-186
Identity = 335/456 (73.46%), Postives = 390/456 (85.53%), Query Frame = 0

Query: 6   VSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLKAG 65
           VS T  ++VFPKTLNP KLLELSNLDRQCPLHMYL+FFYNPS AY++SSLD VFTKLK G
Sbjct: 6   VSRTTSLTVFPKTLNPSKLLELSNLDRQCPLHMYLIFFYNPSSAYTNSSLDSVFTKLKIG 65

Query: 66  LEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYNEFSEKL 125
           LEETL+VW  AAGRLEP+P DGKLNLRC+NRGAIL +A+T+V+IS LGDLS YNEF E+L
Sbjct: 66  LEETLSVWSPAAGRLEPNPADGKLNLRCDNRGAILARAVTDVKISELGDLSNYNEFFEEL 125

Query: 126 VYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIFKQ 185
           V+KPVL+G+F EFPLVVAQ+TRFGCGGY++GTGISHSLFDGPAA++FL+AWA KSAIFK 
Sbjct: 126 VHKPVLDGDFCEFPLVVAQVTRFGCGGYSIGTGISHSLFDGPAAYEFLSAWATKSAIFKD 185

Query: 186 RME-HQVELQRPVHQRGSLL------DMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNG 245
             E ++VELQ+PVH+RG LL        +  R+   S + AVAI+HLY LIMQ+   N  
Sbjct: 186 NKEQNEVELQKPVHERGRLLMGSVPNRCSGKRSAAGSGAEAVAIDHLYKLIMQSAMGNE- 245

Query: 246 EMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVN 305
              + +K EY C T RVSR MI+RLK  A  G N  F CSSFDVIAAHLWKARTKALSV+
Sbjct: 246 ---EMEKKEYGCRTLRVSRGMIERLKKKA-FGENGGFACSSFDVIAAHLWKARTKALSVS 305

Query: 306 KEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSR 365
           KEK VCLQFAVDARN+L PPLPKGFSGN+FVLASVAL AKQLEEES++TI+EMI++AKS+
Sbjct: 306 KEKTVCLQFAVDARNKLKPPLPKGFSGNSFVLASVALPAKQLEEESYKTIIEMIRDAKSQ 365

Query: 366 VNNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEV 425
           V++DYV+AY+LGL+GPQASLPPL ELTV+SDWTRMPFHKI+FLHG+AAYASPLKPPIL+V
Sbjct: 366 VDDDYVNAYQLGLDGPQASLPPLNELTVISDWTRMPFHKIEFLHGEAAYASPLKPPILDV 425

Query: 426 AYFLQNPADSQAIDVRVTLLSHTLDTFSSYFMTSMQ 455
           AYFLQNP DSQAIDVRVTLLS TLD FS+YF+TS+Q
Sbjct: 426 AYFLQNPTDSQAIDVRVTLLSQTLDAFSTYFLTSIQ 456

BLAST of MS008547 vs. TAIR 10
Match: AT4G31910.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.5e-104
Identity = 214/462 (46.32%), Postives = 308/462 (66.67%), Query Frame = 0

Query: 4   SQVSVTKIVSVFPKTLN--PPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTK 63
           +++ + + ++V+P+  N    KL+ LSNLDRQCPL MY VFFY  +   ++   D VF+ 
Sbjct: 7   TRIDIIQKLNVYPRFQNHDKKKLITLSNLDRQCPLLMYSVFFYKNT---TTRDFDSVFSN 66

Query: 64  LKAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAM-TNVEISGLGDLSKYNE 123
           LK GLEET++VW  AAGRL       KLN+RCN+ GA++V+A+ T V++S LGDL++YNE
Sbjct: 67  LKLGLEETMSVWYPAAGRLGLDGGGCKLNIRCNDGGAVMVEAVATGVKLSELGDLTQYNE 126

Query: 124 FSEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKS 183
           F E LVYKP L+G+FS  PLVVAQ+TRF CGGY++G G SHSLFDG +A++F+ AWA+ S
Sbjct: 127 FYENLVYKPSLDGDFSVMPLVVAQVTRFACGGYSIGIGTSHSLFDGISAYEFIHAWASNS 186

Query: 184 AIFKQ---RMEHQVE--LQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQA--- 243
            I  +   ++ ++ E  + +PVH R +LL            + A AI HLY LI QA   
Sbjct: 187 HIHNKSNSKITNKKEDVVIKPVHDRRNLL----VNRDAVRETNAAAICHLYQLIKQAMMT 246

Query: 244 GRENNGEMGKNKKDEYFCTTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKART 303
            +E N  + +     +   TF ++ + I+ +K  +  G    F CSSF+ +AAHLWKART
Sbjct: 247 YQEQNRNL-ELPDSGFVIKTFELNGDAIESMKKKSLEG----FMCSSFEFLAAHLWKART 306

Query: 304 KALSVNKEKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQ-LEEESHRTIVEM 363
           +AL + ++ +VCLQFAVD R R   PLP+GFSGNA+VLASVA TA++ LEE +  +IV  
Sbjct: 307 RALGLRRDAMVCLQFAVDIRKRTETPLPEGFSGNAYVLASVASTARELLEELTLESIVNK 366

Query: 364 IQNAKSRVNNDYVHAYKLGLEGP-QASLPPLKELTVVSDWTRMPFHKIDFLHG--QAAYA 423
           I+ AK  ++  Y+++Y   L G    +LPPLKELT++SDWT+MPFH + F +G   A Y 
Sbjct: 367 IREAKKSIDQGYINSYMEALGGSNDGNLPPLKELTLISDWTKMPFHNVGFGNGGEPADYM 426

Query: 424 SPLKPPILEVAYFLQNPADSQAIDVRVTLLSHTLDTFSSYFM 451
           +PL PP+ +VAYF++NP D++ + VR+ L    ++ FS++F+
Sbjct: 427 APLCPPVPQVAYFMKNPKDAKGVLVRIGLDPRDVNGFSNHFL 456

BLAST of MS008547 vs. TAIR 10
Match: AT5G41040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.6e-43
Identity = 127/451 (28.16%), Postives = 220/451 (48.78%), Query Frame = 0

Query: 3   HSQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKL 62
           H +V   +   V P++     L  LSNLD+   + +  ++ +      +  ++ ++    
Sbjct: 30  HLEVHQKEPALVKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQVI---- 89

Query: 63  KAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYN-EF 122
           K  L + L  +   AGRL  SP +GKL + C   G + V+A  N ++  +GD++K + E 
Sbjct: 90  KKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPET 149

Query: 123 SEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSA 182
             KLVY  V   N  E P V AQ+T+F CGG+ +G  ++H +FDG  A +F+ +W     
Sbjct: 150 LGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWG---- 209

Query: 183 IFKQRMEHQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEMG 242
               ++   + L  P     ++L+    RNP         IE+L+    +   ++N    
Sbjct: 210 ----QVARGLPLTTPPFSDRTILNA---RNPPK-------IENLHQEFEEIEDKSNINSL 269

Query: 243 KNKKDEYFCTTFRVSREMIQRLKNNANLGANAAF--HCSSFDVIAAHLWKARTKALSVNK 302
             K+   +  +F    E I++LK  A   + +     C+SF+ ++A +W+ARTK+L +  
Sbjct: 270 YTKEPTLY-RSFCFDPEKIKKLKLQATENSESLLGNSCTSFEALSAFVWRARTKSLKMLS 329

Query: 303 EKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSRV 362
           ++   L FAVD R +  P LPKG+ GN  VL +    A +L E+     V +++ A   V
Sbjct: 330 DQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAIKMV 389

Query: 363 NNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVA 422
            + Y+ +     E  +A  P L    +++ W+R+ FH  DF  G+   + P+  P  EV 
Sbjct: 390 TDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEKEVT 449

Query: 423 YFLQNPADSQAIDVRVTLLSHTLDTFSSYFM 451
            FL +    ++I+V + L +  +D F   F+
Sbjct: 450 LFLSHGEQRRSINVLLGLPATAMDVFQEQFL 455

BLAST of MS008547 vs. TAIR 10
Match: AT5G41040.2 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 174.1 bits (440), Expect = 2.6e-43
Identity = 127/451 (28.16%), Postives = 220/451 (48.78%), Query Frame = 0

Query: 3   HSQVSVTKIVSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKL 62
           H +V   +   V P++     L  LSNLD+   + +  ++ +      +  ++ ++    
Sbjct: 14  HLEVHQKEPALVKPESETRKGLYFLSNLDQNIAVIVRTIYCFKSEERGNEEAVQVI---- 73

Query: 63  KAGLEETLAVWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGDLSKYN-EF 122
           K  L + L  +   AGRL  SP +GKL + C   G + V+A  N ++  +GD++K + E 
Sbjct: 74  KKALSQVLVHYYPLAGRLTISP-EGKLTVDCTEEGVVFVEAEANCKMDEIGDITKPDPET 133

Query: 123 SEKLVYKPVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSA 182
             KLVY  V   N  E P V AQ+T+F CGG+ +G  ++H +FDG  A +F+ +W     
Sbjct: 134 LGKLVYDVVDAKNILEIPPVTAQVTKFKCGGFVLGLCMNHCMFDGIGAMEFVNSWG---- 193

Query: 183 IFKQRMEHQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEMG 242
               ++   + L  P     ++L+    RNP         IE+L+    +   ++N    
Sbjct: 194 ----QVARGLPLTTPPFSDRTILNA---RNPPK-------IENLHQEFEEIEDKSNINSL 253

Query: 243 KNKKDEYFCTTFRVSREMIQRLKNNANLGANAAF--HCSSFDVIAAHLWKARTKALSVNK 302
             K+   +  +F    E I++LK  A   + +     C+SF+ ++A +W+ARTK+L +  
Sbjct: 254 YTKEPTLY-RSFCFDPEKIKKLKLQATENSESLLGNSCTSFEALSAFVWRARTKSLKMLS 313

Query: 303 EKIVCLQFAVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSRV 362
           ++   L FAVD R +  P LPKG+ GN  VL +    A +L E+     V +++ A   V
Sbjct: 314 DQKTKLLFAVDGRAKFEPQLPKGYFGNGIVLTNSICEAGELIEKPLSFAVGLVREAIKMV 373

Query: 363 NNDYVHAYKLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVA 422
            + Y+ +     E  +A  P L    +++ W+R+ FH  DF  G+   + P+  P  EV 
Sbjct: 374 TDGYMRSAIDYFEVTRAR-PSLSSTLLITTWSRLGFHTTDFGWGEPILSGPVALPEKEVT 433

Query: 423 YFLQNPADSQAIDVRVTLLSHTLDTFSSYFM 451
            FL +    ++I+V + L +  +D F   F+
Sbjct: 434 LFLSHGEQRRSINVLLGLPATAMDVFQEQFL 439

BLAST of MS008547 vs. TAIR 10
Match: AT3G48720.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 162.9 bits (411), Expect = 5.9e-40
Identity = 120/429 (27.97%), Postives = 204/429 (47.55%), Query Frame = 0

Query: 27  LSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLKAGLEETLAVWPQAAGRLEPSPTD 86
           LSNLD+   + +  +++Y      +  S +++    K  L E L  +   AGRL  SP +
Sbjct: 30  LSNLDQNIAIIVKTLYYYKSESRTNQESYNVI----KKSLSEVLVHYYPVAGRLTISP-E 89

Query: 87  GKLNLRCNNRGAILVQAMTNVEISGLGDLSKYN--EFSEKLVYKPVLNGNFSEFPLVVAQ 146
           GK+ + C   G ++V+A  N  I  + +    N  E  EKLVY      N  E P VV Q
Sbjct: 90  GKIAVNCTGEGVVVVEAEANCGIDTIKEAISENRMETLEKLVYDVPGARNILEIPPVVVQ 149

Query: 147 LTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIFKQRMEHQVELQRPVHQRGSLL 206
           +T F CGG+ +G G+SH++FDG AA +FL +W          M   + L  P     ++L
Sbjct: 150 VTNFKCGGFVLGLGMSHNMFDGVAAAEFLNSWC--------EMAKGLPLSVPPFLDRTIL 209

Query: 207 DMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEMGK-NKKDEYFCTTFRVSREMIQRL 266
              + RNP          + +         E+  + GK   +++    +F    E +++L
Sbjct: 210 ---RSRNPPKIEFPHNEFDEI---------EDISDTGKIYDEEKLIYKSFLFEPEKLEKL 269

Query: 267 KNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKEKIVCLQFAVDARNRLLPPLPKGF 326
           K  A +  N     S+F  +   LWK+R +AL    ++ V L FA D R+R +P LP+G+
Sbjct: 270 KIMA-IEENNNNKVSTFQALTGFLWKSRCEALRFKPDQRVKLLFAADGRSRFIPRLPQGY 329

Query: 327 SGNAFVLASVALTAKQL--EEESH-----RTIVEMIQNAKSRVNNDYVHAYKLGLEGPQA 386
            GN  VL  +  ++ +L     SH     + +VE++ +   R   DY    +        
Sbjct: 330 CGNGIVLTGLVTSSGELVGNPLSHSVGLVKRLVELVTDGFMRSAMDYFEVNR-------- 389

Query: 387 SLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAYFLQNPADSQAIDVRVT 446
           + P +    +++ W+++  HK+DF  G+  ++ P+  P  EV  FL +  D ++I+V + 
Sbjct: 390 TRPSMNATLLITSWSKLTLHKLDFGWGEPVFSGPVGLPGREVILFLPSGDDMKSINVFLG 424

BLAST of MS008547 vs. TAIR 10
Match: AT5G63560.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 162.2 bits (409), Expect = 1.0e-39
Identity = 127/438 (29.00%), Postives = 204/438 (46.58%), Query Frame = 0

Query: 12  VSVFPKTLNPPKLLELSNLDRQCPLHMYLVFFYNPSPAYSSSSLDLVFTKLKAGLEETLA 71
           V V P +  P  L  LSNLD+   + +   +++  +   +  S +++    K  L E L 
Sbjct: 15  VLVSPASETPKGLHYLSNLDQNIAIIVKTFYYFKSNSRSNEESYEVI----KKSLSEVLV 74

Query: 72  VWPQAAGRLEPSPTDGKLNLRCNNRGAILVQAMTNVEISGLGD-LSKYN--EFSEKLVYK 131
            +  AAGRL  SP +GK+ + C   G ++V+A  N  I  +   +S+ +  E  EKLVY 
Sbjct: 75  HYYPAAGRLTISP-EGKIAVDCTGEGVVVVEAEANCGIEKIKKAISEIDQPETLEKLVYD 134

Query: 132 PVLNGNFSEFPLVVAQLTRFGCGGYTVGTGISHSLFDGPAAHDFLTAWAAKSAIFKQRME 191
                N  E P VV Q+T F CGG+ +G G++H++FDG AA +FL +WA  +        
Sbjct: 135 VPGARNILEIPPVVVQVTNFKCGGFVLGLGMNHNMFDGIAAMEFLNSWAETA-------- 194

Query: 192 HQVELQRPVHQRGSLLDMAKFRNPRSSASAAVAIEHLYGLIMQAGRENNGEMGKNKKDEY 251
             + L  P     +LL       PR+             L      E+    GK   DE 
Sbjct: 195 RGLPLSVPPFLDRTLL------RPRTPPKIEFPHNEFEDL------EDISGTGKLYSDEK 254

Query: 252 FC-TTFRVSREMIQRLKNNANLGANAAFHCSSFDVIAAHLWKARTKALSVNKEKIVCLQF 311
               +F    E ++RLK        A    ++F  +   LW+AR +AL +  ++ + L F
Sbjct: 255 LVYKSFLFGPEKLERLK------IMAETRSTTFQTLTGFLWRARCQALGLKPDQRIKLLF 314

Query: 312 AVDARNRLLPPLPKGFSGNAFVLASVALTAKQLEEESHRTIVEMIQNAKSRVNNDYVHAY 371
           A D R+R +P LPKG+SGN  V      TA ++        V +++ A   VN+ ++ + 
Sbjct: 315 AADGRSRFVPELPKGYSGNGIVFTYCVTTAGEVTLNPLSHSVCLVKRAVEMVNDGFMRSA 374

Query: 372 KLGLEGPQASLPPLKELTVVSDWTRMPFHKIDFLHGQAAYASPLKPPILEVAYFLQNPAD 431
               E  +A  P L    +++ W ++ FH  DF  G+   + P+  P  EV  FL   +D
Sbjct: 375 IDYFEVTRAR-PSLTATLLITSWAKLSFHTKDFGWGEPVVSGPVGLPEKEVILFLPCGSD 420

Query: 432 SQAIDVRVTLLSHTLDTF 446
           +++I+V + L    +  F
Sbjct: 435 TKSINVLLGLPGSAMKVF 420