MS008543 (gene) Bitter gourd (TR) v1

Overview


Name	MS008543
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	PPPDE domain-containing protein
Location	scaffold4: 2104897 .. 2105891 (-)
RNA-Seq Expression	MS008543
Synteny	MS008543

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCATGGAGTTGAATATGCGTTTGGAGCACATGATCATGCAACTTCAGGGATATTTGAGGTAGAACCAAGACAATGTCCTGGTTTTACATTTAGGAAATCAATTTGCATAGGACGAACGAATCTCGGTCCAAAAGAGGTCCGATCGTTTATGGAGAAACTAGCCGAAGAGTACTCTGGCAACACTTATCATTTGATAACAAAAAATTGCAACCACTTTTGCAACGATGTGTGCGTAAGGTTGGCAGGAAAACCAATCCCTAGCTGGGTCAACCGTCTTGCCCGACTTGGTAAGATTGATGATTTTATTCACTGCATATGATATAACCGTTTTCTTTCCATAATTGGGTTCTGTTCTATGCTTAAATTTTGATTACCCAGCATGCTGCTACTTGTGATTGTTGTGTCATTCATACAAACTTTTCCATAAGTTTAAAAACAAACTGCCCATCGTAACCACTGAGTTTCTATGTCTTGATCTTATGAAAAATTTGGCTTGGCTTCCCCTGGTATCAAATTTTTGAAAGACAAACATGGCGAACGGGGAACGCTTCGTTTGCTCCCTTTACTACCTCGTGCACATCACTGAGATTGAAACTTACAACAATTCGTGACGTTGGTTTGATATGTTTTGTGTTTTCAGGTCTTTTCTGCAACTGTGTTCTTCCAGCGAGTTTGAACGAAGCGAAGGTTCGGCAAGTAAGACCAGGAGGTAGGATTAGCCAAGGTGTAAAGAAGCAATTGAGAAGCAACCCGCATAGCGAAGGTGTAAAGAAGCAATTGAGAAGCAACCCAGCTAGCGAAGGTGTAAAGAAGCAATTGAGAAGCAACTCAAGTAGGCATAATAACGAATCTCCTTCCAACTCTTCACCACCAACATTGTCATCCAATCCTCCAAGTTCATCAACCATCAAAGTAAGTAGACAAAAACGATGCCTCCCGCCATCTTCATCGCTGATTCATTCTTCATCAAATGCAGCCCTGACTATAAAGCTC

mRNA sequence

GTTCATGGAGTTGAATATGCGTTTGGAGCACATGATCATGCAACTTCAGGGATATTTGAGGTAGAACCAAGACAATGTCCTGGTTTTACATTTAGGAAATCAATTTGCATAGGACGAACGAATCTCGGTCCAAAAGAGGTCCGATCGTTTATGGAGAAACTAGCCGAAGAGTACTCTGGCAACACTTATCATTTGATAACAAAAAATTGCAACCACTTTTGCAACGATGTGTGCGTAAGGTTGGCAGGAAAACCAATCCCTAGCTGGGTCAACCGTCTTGCCCGACTTGGTCTTTTCTGCAACTGTGTTCTTCCAGCGAGTTTGAACGAAGCGAAGGTTCGGCAAGTAAGACCAGGAGGTAGGATTAGCCAAGGTGTAAAGAAGCAATTGAGAAGCAACCCGCATAGCGAAGGTGTAAAGAAGCAATTGAGAAGCAACCCAGCTAGCGAAGGTGTAAAGAAGCAATTGAGAAGCAACTCAAGTAGGCATAATAACGAATCTCCTTCCAACTCTTCACCACCAACATTGTCATCCAATCCTCCAAGTTCATCAACCATCAAAGTAAGTAGACAAAAACGATGCCTCCCGCCATCTTCATCGCTGATTCATTCTTCATCAAATGCAGCCCTGACTATAAAGCTC

Coding sequence (CDS)

GTTCATGGAGTTGAATATGCGTTTGGAGCACATGATCATGCAACTTCAGGGATATTTGAGGTAGAACCAAGACAATGTCCTGGTTTTACATTTAGGAAATCAATTTGCATAGGACGAACGAATCTCGGTCCAAAAGAGGTCCGATCGTTTATGGAGAAACTAGCCGAAGAGTACTCTGGCAACACTTATCATTTGATAACAAAAAATTGCAACCACTTTTGCAACGATGTGTGCGTAAGGTTGGCAGGAAAACCAATCCCTAGCTGGGTCAACCGTCTTGCCCGACTTGGTCTTTTCTGCAACTGTGTTCTTCCAGCGAGTTTGAACGAAGCGAAGGTTCGGCAAGTAAGACCAGGAGGTAGGATTAGCCAAGGTGTAAAGAAGCAATTGAGAAGCAACCCGCATAGCGAAGGTGTAAAGAAGCAATTGAGAAGCAACCCAGCTAGCGAAGGTGTAAAGAAGCAATTGAGAAGCAACTCAAGTAGGCATAATAACGAATCTCCTTCCAACTCTTCACCACCAACATTGTCATCCAATCCTCCAAGTTCATCAACCATCAAAGTAAGTAGACAAAAACGATGCCTCCCGCCATCTTCATCGCTGATTCATTCTTCATCAAATGCAGCCCTGACTATAAAGCTC

Protein sequence

VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSGNTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGGRISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNPPSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL

Homology

[Close]

BLAST of MS008543 vs. NCBI nr
Match: XP_022141433.1 (deSI-like protein At4g17486 isoform X2 [Momordica charantia])

HSP 1 Score: 418.7 bits (1075), Expect = 3.0e-113
Identity = 214/227 (94.27%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG
Sbjct: 50  VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 109

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG
Sbjct: 110 NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 169

Query: 121 RISQ-------------GVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES 180
           RISQ             GVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES
Sbjct: 170 RISQGVKKQLRSNPHSEGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES 229

Query: 181 PSNSSPPTLSSNPPSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL 215
           PSNSSPPTLSSNPPSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL
Sbjct: 230 PSNSSPPTLSSNPPSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL 276

[Close]

BLAST of MS008543 vs. NCBI nr
Match: XP_022141442.1 (deSI-like protein At4g17486 isoform X3 [Momordica charantia])

HSP 1 Score: 381.7 bits (979), Expect = 4.1e-102
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG
Sbjct: 50  VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 109

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG
Sbjct: 110 NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 169

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 180
           RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP
Sbjct: 170 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 229

Query: 181 PSSSTIKV 189
           PSSSTIKV
Sbjct: 230 PSSSTIKV 237

[Close]

BLAST of MS008543 vs. NCBI nr
Match: XP_022141415.1 (deSI-like protein At4g17486 isoform X1 [Momordica charantia] >XP_022141423.1 deSI-like protein At4g17486 isoform X1 [Momordica charantia])

HSP 1 Score: 372.5 bits (955), Expect = 2.5e-99
Identity = 188/201 (93.53%), Postives = 188/201 (93.53%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG
Sbjct: 50  VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 109

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG
Sbjct: 110 NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 169

Query: 121 RISQ-------------GVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES 180
           RISQ             GVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES
Sbjct: 170 RISQGVKKQLRSNPHSEGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES 229

Query: 181 PSNSSPPTLSSNPPSSSTIKV 189
           PSNSSPPTLSSNPPSSSTIKV
Sbjct: 230 PSNSSPPTLSSNPPSSSTIKV 250

[Close]

BLAST of MS008543 vs. NCBI nr
Match: XP_004145512.1 (deSI-like protein At4g17486 [Cucumis sativus] >KGN55449.1 hypothetical protein Csa_012914 [Cucumis sativus])

HSP 1 Score: 314.3 bits (804), Expect = 8.0e-82
Identity = 157/214 (73.36%), Postives = 174/214 (81.31%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAH+HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPK+VRSFMEKLAEEYSG
Sbjct: 47  VHGVEYAFGAHEHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSG 106

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLIT+NCNHFCNDVC+RLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVR G 
Sbjct: 107 NTYHLITRNCNHFCNDVCIRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGD 166

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 180
           ++++GVKK+L+S P                           R+NN S SN   P LSSNP
Sbjct: 167 KLNEGVKKKLKSQP--------------------------IRYNNTSSSNPPRPPLSSNP 226

Query: 181 PSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL 215
           P+SSTIKVSRQKRC+P +SS IHSS++  LT+KL
Sbjct: 227 PTSSTIKVSRQKRCIPSASSAIHSSASTTLTVKL 234

[Close]

BLAST of MS008543 vs. NCBI nr
Match: KAA0064599.1 (deSI-like protein [Cucumis melo var. makuwa] >TYK19991.1 deSI-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 309.3 bits (791), Expect = 2.6e-80
Identity = 157/214 (73.36%), Postives = 173/214 (80.84%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAH+HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG
Sbjct: 66  VHGVEYAFGAHEHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 125

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLIT+NCNHFCNDVC+RLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVR G 
Sbjct: 126 NTYHLITRNCNHFCNDVCIRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGD 185

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 180
           ++++GVKK+LRS P                          +R NN+S SN SPP L SNP
Sbjct: 186 KLNEGVKKKLRSQP--------------------------NRCNNKSSSNPSPPPLPSNP 245

Query: 181 PSSSTIKV---SRQKRCLPPSSSLIHSSSNAALT 212
           P+SSTIKV   +RQKRC+P +SS IHSS++  LT
Sbjct: 246 PTSSTIKVTRPTRQKRCIPSASSAIHSSASTTLT 253

[Close]

BLAST of MS008543 vs. ExPASy Swiss-Prot
Match: Q93VG8 (DeSI-like protein At4g17486 OS=Arabidopsis thaliana OX=3702 GN=At4g17486 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 7.9e-40
Identity = 64/107 (59.81%), Postives = 87/107 (81.31%), Query Frame = 0


Query: 2   HGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSGN 61
           H +EY +GAH++ TSG++EVEPR CPGF FR+S+ +G T++   + RS+MEKL+ +Y G+
Sbjct: 57  HNLEYCYGAHEYPTSGVYEVEPRNCPGFIFRRSVLLGTTSMSRSDFRSYMEKLSRKYHGD 116

Query: 62  TYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASL 109
           TYHLI KNCNHF  +VC++L GKPIP W+NRLAR+G FCNC+LP S+
Sbjct: 117 TYHLIAKNCNHFTEEVCLQLTGKPIPGWINRLARVGSFCNCLLPESI 163

[Close]

BLAST of MS008543 vs. ExPASy Swiss-Prot
Match: H2KZK4 (Desumoylating isopeptidase 1 homolog OS=Caenorhabditis elegans OX=6239 GN=F36D4.5 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-19
Identity = 43/100 (43.00%), Postives = 61/100 (61.00%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQC----PGFTFRKSICIGRTNLGPKEVRSFMEKLAE 60
           V GVEYA+G H +  SG+FE  P+        F F++SI +G T     ++R  ++ L E
Sbjct: 60  VFGVEYAYGGHPYQFSGVFENSPQDAEELGETFKFKESIVVGETERSTSDIRKLIKSLGE 119

Query: 61  EYSGNTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARL 97
           ++ G+ YHLI++NCNHF   +   L GK IP W+NRLA L
Sbjct: 120 DFRGDRYHLISRNCNHFSAVLARELTGKDIPGWINRLANL 159

[Close]

BLAST of MS008543 vs. ExPASy Swiss-Prot
Match: Q5PQ09 (Deubiquitinase DESI2 OS=Xenopus laevis OX=8355 GN=desi2 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.6e-19
Identity = 45/107 (42.06%), Postives = 64/107 (59.81%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQC----PGFTFRKSICIGRTNLGPKEVRSFMEKLAE 60
           V+G E+A+G H +  SG+FE+ P         F F+++I +G T+    ++   +E+L +
Sbjct: 34  VYGREFAYGGHPYPFSGVFEISPGDSTELGDTFKFKEAIALGSTDFTENDIEKIIEELGK 93

Query: 61  EYSGNTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCV 104
           EY GN YHL+ KNCNHF + +   L GK IP WVNRLA    F  CV
Sbjct: 94  EYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWVNRLA---YFSTCV 137

[Close]

BLAST of MS008543 vs. ExPASy Swiss-Prot
Match: Q6DC39 (Deubiquitinase DESI2 OS=Danio rerio OX=7955 GN=desi2 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.5e-19
Identity = 44/107 (41.12%), Postives = 66/107 (61.68%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQC----PGFTFRKSICIGRTNLGPKEVRSFMEKLAE 60
           ++G E+A+G H +  SGIFE+ P         F F+++I +G T+   ++V   +E++ +
Sbjct: 34  IYGREFAYGGHPYPFSGIFEITPGDATELGETFKFKEAIVLGSTDFTEEDVERIVEEMGK 93

Query: 61  EYSGNTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCV 104
           EY GN YHL+ KNCNHF + +   L G+ IP WVNRLA    F +CV
Sbjct: 94  EYKGNAYHLMHKNCNHFSSALSEILCGREIPRWVNRLA---YFSSCV 137

[Close]

BLAST of MS008543 vs. ExPASy Swiss-Prot
Match: Q9BSY9 (Deubiquitinase DESI2 OS=Homo sapiens OX=9606 GN=DESI2 PE=1 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 4.5e-19
Identity = 43/107 (40.19%), Postives = 65/107 (60.75%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQC----PGFTFRKSICIGRTNLGPKEVRSFMEKLAE 60
           V+G E+A+G H +  SGIFE+ P         F F++++ +G T+    ++   +E+L +
Sbjct: 35  VYGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGK 94

Query: 61  EYSGNTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCV 104
           EY GN YHL+ KNCNHF + +   L GK IP W+NRLA    F +C+
Sbjct: 95  EYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLA---YFSSCI 138

[Close]

BLAST of MS008543 vs. ExPASy TrEMBL
Match: A0A6J1CKH1 (deSI-like protein At4g17486 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011821 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.5e-113
Identity = 214/227 (94.27%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG
Sbjct: 50  VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 109

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG
Sbjct: 110 NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 169

Query: 121 RISQ-------------GVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES 180
           RISQ             GVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES
Sbjct: 170 RISQGVKKQLRSNPHSEGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES 229

Query: 181 PSNSSPPTLSSNPPSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL 215
           PSNSSPPTLSSNPPSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL
Sbjct: 230 PSNSSPPTLSSNPPSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL 276

[Close]

BLAST of MS008543 vs. ExPASy TrEMBL
Match: A0A6J1CJV3 (deSI-like protein At4g17486 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011821 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.0e-102
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG
Sbjct: 50  VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 109

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG
Sbjct: 110 NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 169

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 180
           RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP
Sbjct: 170 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 229

Query: 181 PSSSTIKV 189
           PSSSTIKV
Sbjct: 230 PSSSTIKV 237

[Close]

BLAST of MS008543 vs. ExPASy TrEMBL
Match: A0A6J1CKG3 (deSI-like protein At4g17486 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011821 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.2e-99
Identity = 188/201 (93.53%), Postives = 188/201 (93.53%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG
Sbjct: 50  VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 109

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG
Sbjct: 110 NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 169

Query: 121 RISQ-------------GVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES 180
           RISQ             GVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES
Sbjct: 170 RISQGVKKQLRSNPHSEGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNES 229

Query: 181 PSNSSPPTLSSNPPSSSTIKV 189
           PSNSSPPTLSSNPPSSSTIKV
Sbjct: 230 PSNSSPPTLSSNPPSSSTIKV 250

[Close]

BLAST of MS008543 vs. ExPASy TrEMBL
Match: A0A0A0L339 (PPPDE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652130 PE=3 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 3.9e-82
Identity = 157/214 (73.36%), Postives = 174/214 (81.31%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAH+HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPK+VRSFMEKLAEEYSG
Sbjct: 47  VHGVEYAFGAHEHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKDVRSFMEKLAEEYSG 106

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLIT+NCNHFCNDVC+RLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVR G 
Sbjct: 107 NTYHLITRNCNHFCNDVCIRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGD 166

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 180
           ++++GVKK+L+S P                           R+NN S SN   P LSSNP
Sbjct: 167 KLNEGVKKKLKSQP--------------------------IRYNNTSSSNPPRPPLSSNP 226

Query: 181 PSSSTIKVSRQKRCLPPSSSLIHSSSNAALTIKL 215
           P+SSTIKVSRQKRC+P +SS IHSS++  LT+KL
Sbjct: 227 PTSSTIKVSRQKRCIPSASSAIHSSASTTLTVKL 234

[Close]

BLAST of MS008543 vs. ExPASy TrEMBL
Match: A0A5A7VGG1 (DeSI-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001070 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.3e-80
Identity = 157/214 (73.36%), Postives = 173/214 (80.84%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAH+HATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG
Sbjct: 66  VHGVEYAFGAHEHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 125

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           NTYHLIT+NCNHFCNDVC+RLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVR G 
Sbjct: 126 NTYHLITRNCNHFCNDVCIRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRSGD 185

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 180
           ++++GVKK+LRS P                          +R NN+S SN SPP L SNP
Sbjct: 186 KLNEGVKKKLRSQP--------------------------NRCNNKSSSNPSPPPLPSNP 245

Query: 181 PSSSTIKV---SRQKRCLPPSSSLIHSSSNAALT 212
           P+SSTIKV   +RQKRC+P +SS IHSS++  LT
Sbjct: 246 PTSSTIKVTRPTRQKRCIPSASSAIHSSASTTLT 253

[Close]

BLAST of MS008543 vs. TAIR 10
Match: AT5G25170.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 221.5 bits (563), Expect = 6.6e-58
Identity = 117/201 (58.21%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEY FGAHDH+T+GIFEVEP+QCPGFTFRKSI IGRT+L P+ VR FMEKLAEEYSG
Sbjct: 48  VHGVEYGFGAHDHSTTGIFEVEPKQCPGFTFRKSILIGRTDLDPENVRVFMEKLAEEYSG 107

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           N+YHLITKNCNHFCNDVCV+L  + IPSWVNRLAR GLFCNCVLPA LNE KVRQVR   
Sbjct: 108 NSYHLITKNCNHFCNDVCVQLTRRSIPSWVNRLARFGLFCNCVLPAELNETKVRQVRS-- 167

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 180
                                  +     E  KK+LRS SSR           P+LSS+ 
Sbjct: 168 -----------------------KEEKIPEVEKKKLRSRSSRF-------PPGPSLSSSG 216

Query: 181 PSSSTIKVSRQKRCLPPSSSL 202
             + + +  R+++CLPPS ++
Sbjct: 228 SLNRSRRGERRRQCLPPSPTV 216

[Close]

BLAST of MS008543 vs. TAIR 10
Match: AT2G25190.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 207.6 bits (527), Expect = 9.9e-54
Identity = 105/187 (56.15%), Postives = 137/187 (73.26%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAH+ +++GIFEVEP++CPGFTFRKSI +G+T+L  KEVR FMEKLAEEY G
Sbjct: 47  VHGVEYAFGAHESSSTGIFEVEPKKCPGFTFRKSILVGKTDLVAKEVRVFMEKLAEEYQG 106

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           N YHLIT+NCNHFCN+VC++LA K IP WVNRLARLG+ CNCVLP  LNEAKVR+V   G
Sbjct: 107 NKYHLITRNCNHFCNEVCLKLAQKSIPRWVNRLARLGVLCNCVLPPRLNEAKVRRVGK-G 166

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLSSNP 180
            +S+  KK+LR+   S+ +     S+   +  +  +R+ SS ++  S S+SS  +  +  
Sbjct: 167 ELSESEKKKLRNRSRSDPLLSSSPSSSTPDNHRSHIRAKSSGNHPSSSSSSSSGSKKNRR 226

Query: 181 PSSSTIK 188
           P +   K
Sbjct: 227 PKAQDQK 232

[Close]

BLAST of MS008543 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 194.5 bits (493), Expect = 8.7e-50
Identity = 102/183 (55.74%), Postives = 126/183 (68.85%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEY +GAH+ ++SGIFEVEP++CPGFTFRKSI +G T +  KEVRSFMEKL+EEY G
Sbjct: 47  VHGVEYGYGAHEKSSSGIFEVEPKKCPGFTFRKSILVGETEMKAKEVRSFMEKLSEEYQG 106

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           N YHLIT+NCNHFCN V ++L  K IPSWVNRLARLG  CNCVLPA LNE KV++V   G
Sbjct: 107 NKYHLITRNCNHFCNHVSLKLTHKSIPSWVNRLARLGFLCNCVLPACLNETKVKRVGKDG 166

Query: 121 RI---SQGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLS 180
           ++    +  KK+ R         ++ RS P S         N+  H N S S  +PP LS
Sbjct: 167 KLLLEGENTKKKKRKKK-----IRRSRSGPLSSSSSNARLDNTPTH-NRSISTGNPP-LS 222

[Close]

BLAST of MS008543 vs. TAIR 10
Match: AT1G80690.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 176.8 bits (447), Expect = 1.9e-44
Identity = 92/182 (50.55%), Postives = 123/182 (67.58%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHG+EYA+GAH++ ++GIFE EP+QC GFTFRKSI IG+T+LGP EVR+ ME+LA+ Y G
Sbjct: 45  VHGIEYAYGAHEYPSTGIFEGEPKQCEGFTFRKSILIGKTDLGPLEVRATMEQLADNYKG 104

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           ++Y+LITKNCNHFC++ C++L G PIPSWVNRLAR+G  CNCVLPA++N  +    R   
Sbjct: 105 SSYNLITKNCNHFCDETCIKLTGNPIPSWVNRLARIGFMCNCVLPATINATRFGNNRVNQ 164

Query: 121 RIS---QGVKKQLRSNPHSEGVKKQLRSNPASEGVKKQLRSNSSRHNNESPSNSSPPTLS 180
             S   +  KK+L S    E       S+ +S     Q+R  S +    +   SSP TL 
Sbjct: 165 DKSCEAENEKKKLTSVSSRERSTIATPSS-SSSSPSVQVRGRSRKRRPRALQPSSPLTLG 224

[Close]

BLAST of MS008543 vs. TAIR 10
Match: AT1G47740.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 173.7 bits (439), Expect = 1.6e-43
Identity = 98/191 (51.31%), Postives = 120/191 (62.83%), Query Frame = 0


Query: 1   VHGVEYAFGAHDHATSGIFEVEPRQCPGFTFRKSICIGRTNLGPKEVRSFMEKLAEEYSG 60
           VHGVEYAFGAHD+ATSG+FEVEPRQCPGF F+KSI IG TNL P +VR FME +A  Y G
Sbjct: 99  VHGVEYAFGAHDYATSGVFEVEPRQCPGFKFKKSIFIGTTNLNPTQVREFMEDMACSYYG 158

Query: 61  NTYHLITKNCNHFCNDVCVRLAGKPIPSWVNRLARLGLFCNCVLPASLNEAKVRQVRPGG 120
           N YHLI KNCNHFC DVC +L GK IP WVNRLA++G  C+C+LP SL    V    P G
Sbjct: 159 NMYHLIVKNCNHFCQDVCYKLTGKKIPKWVNRLAQIGSVCSCILPESLKITAVCH-DPDG 218

Query: 121 RISQGVKKQLRSNPHSEGVKKQLRSN---PASEGVKKQLRSNSSRHNNESPSNSSPP-TL 180
           +I           P  E  K+ LRS+    +S  ++++  S SS           PP  L
Sbjct: 219 QI-----------PEEENEKRSLRSSFSCLSSISMRQKQLSTSSLFLQSPLRGCLPPWQL 277

Query: 181 SSNPPSSSTIK 188
             +  +SS++K
Sbjct: 279 KRSKSNSSSLK 277

The following BLAST results are available for this feature:

BLAST of MS008543 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141433.1	3.0e-113	94.27	deSI-like protein At4g17486 isoform X2 [Momordica charantia]	[more]
XP_022141442.1	4.1e-102	100.00	deSI-like protein At4g17486 isoform X3 [Momordica charantia]	[more]
XP_022141415.1	2.5e-99	93.53	deSI-like protein At4g17486 isoform X1 [Momordica charantia] >XP_022141423.1 deS...	[more]
XP_004145512.1	8.0e-82	73.36	deSI-like protein At4g17486 [Cucumis sativus] >KGN55449.1 hypothetical protein C...	[more]
KAA0064599.1	2.6e-80	73.36	deSI-like protein [Cucumis melo var. makuwa] >TYK19991.1 deSI-like protein [Cucu...	[more]

BLAST of MS008543 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VG8	7.9e-40	59.81	DeSI-like protein At4g17486 OS=Arabidopsis thaliana OX=3702 GN=At4g17486 PE=2 SV...	[more]
H2KZK4	1.2e-19	43.00	Desumoylating isopeptidase 1 homolog OS=Caenorhabditis elegans OX=6239 GN=F36D4....	[more]
Q5PQ09	1.6e-19	42.06	Deubiquitinase DESI2 OS=Xenopus laevis OX=8355 GN=desi2 PE=2 SV=1	[more]
Q6DC39	4.5e-19	41.12	Deubiquitinase DESI2 OS=Danio rerio OX=7955 GN=desi2 PE=2 SV=1	[more]
Q9BSY9	4.5e-19	40.19	Deubiquitinase DESI2 OS=Homo sapiens OX=9606 GN=DESI2 PE=1 SV=1	[more]

BLAST of MS008543 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKH1	1.5e-113	94.27	deSI-like protein At4g17486 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CJV3	2.0e-102	100.00	deSI-like protein At4g17486 isoform X3 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CKG3	1.2e-99	93.53	deSI-like protein At4g17486 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0L339	3.9e-82	73.36	PPPDE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G652130 PE=3 ...	[more]
A0A5A7VGG1	1.3e-80	73.36	DeSI-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00...	[more]

BLAST of MS008543 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25170.1	6.6e-58	58.21	PPPDE putative thiol peptidase family protein	[more]
AT2G25190.1	9.9e-54	56.15	PPPDE putative thiol peptidase family protein	[more]
AT4G31980.1	8.7e-50	55.74	unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,...	[more]
AT1G80690.1	1.9e-44	50.55	PPPDE putative thiol peptidase family protein	[more]
AT1G47740.1	1.6e-43	51.31	PPPDE putative thiol peptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008580	PPPDE peptidase domain	SMART	SM01179	DUF862_2a	coord: 1..109 e-value: 2.8E-23 score: 93.3
IPR008580	PPPDE peptidase domain	PFAM	PF05903	Peptidase_C97	coord: 1..106 e-value: 2.0E-37 score: 128.4
IPR008580	PPPDE peptidase domain	PANTHER	PTHR12378	DESUMOYLATING ISOPEPTIDASE	coord: 1..190
IPR008580	PPPDE peptidase domain	PROSITE	PS51858	PPPDE	coord: 1..108 score: 48.297939
IPR042266	PPPDE peptidase domain superfamily	GENE3D	3.90.1720.30	PPPDE domains	coord: 1..118 e-value: 1.7E-35 score: 124.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 119..214
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 161..214
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 139..155
None	No IPR available	PANTHER	PTHR12378:SF17	OS02G0788133 PROTEIN	coord: 1..190
IPR004166	MHCK/EF2 kinase	PROSITE	PS51158	ALPHA_KINASE	coord: 1..86 score: 8.721237

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008543.1	MS008543.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity