MS008536 (gene) Bitter gourd (TR) v1

Overview


Name	MS008536
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA ligase
Location	scaffold4: 2046224 .. 2046580 (+)
RNA-Seq Expression	MS008536
Synteny	MS008536

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGAACAACGAGTACAATTCATCTTCATTTTCCTTCACTCACAAGCTCAAATCCTCCATCCTCTGCTTCCGCGCCGACCATGGCGATTTGGGAGATCACGAAGACGATGCCTCTCTAAGGAGCCCTAGATCGCCGCTCAAATCCACGGCCAGCGAGCATACTGAGCTCCGGGGAATGTGCCGGAATCTGGTGGCGAGGATCGGCCGGAGCCGGCGGCGGTACCATCACTCCGCGGATTTCAGATACGATCCTTCTAGCTATGCTCTTAATTTCGAGGACGAACACGCTCGCTACGATGACGAATTTCCGATTAGAGATTTCGCTTCGAGATTGCCGGCGTCGCCGCATTCTTCC

mRNA sequence

ATGTTGAACAACGAGTACAATTCATCTTCATTTTCCTTCACTCACAAGCTCAAATCCTCCATCCTCTGCTTCCGCGCCGACCATGGCGATTTGGGAGATCACGAAGACGATGCCTCTCTAAGGAGCCCTAGATCGCCGCTCAAATCCACGGCCAGCGAGCATACTGAGCTCCGGGGAATGTGCCGGAATCTGGTGGCGAGGATCGGCCGGAGCCGGCGGCGGTACCATCACTCCGCGGATTTCAGATACGATCCTTCTAGCTATGCTCTTAATTTCGAGGACGAACACGCTCGCTACGATGACGAATTTCCGATTAGAGATTTCGCTTCGAGATTGCCGGCGTCGCCGCATTCTTCC

Coding sequence (CDS)

ATGTTGAACAACGAGTACAATTCATCTTCATTTTCCTTCACTCACAAGCTCAAATCCTCCATCCTCTGCTTCCGCGCCGACCATGGCGATTTGGGAGATCACGAAGACGATGCCTCTCTAAGGAGCCCTAGATCGCCGCTCAAATCCACGGCCAGCGAGCATACTGAGCTCCGGGGAATGTGCCGGAATCTGGTGGCGAGGATCGGCCGGAGCCGGCGGCGGTACCATCACTCCGCGGATTTCAGATACGATCCTTCTAGCTATGCTCTTAATTTCGAGGACGAACACGCTCGCTACGATGACGAATTTCCGATTAGAGATTTCGCTTCGAGATTGCCGGCGTCGCCGCATTCTTCC

Protein sequence

MLNNEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGMCRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS

Homology

[Close]

BLAST of MS008536 vs. NCBI nr
Match: XP_022138148.1 (uncharacterized protein LOC111009386 [Momordica charantia])

HSP 1 Score: 238.8 bits (608), Expect = 2.4e-59
Identity = 116/119 (97.48%), Postives = 118/119 (99.16%), Query Frame = 0


Query: 1   MLNNEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGM 60
           MLNNEYNSS FSFTHKLKSSILCFRADHGDLGDHED+ASLRSPRSPLKSTASEHTELRGM
Sbjct: 1   MLNNEYNSSPFSFTHKLKSSILCFRADHGDLGDHEDEASLRSPRSPLKSTASEHTELRGM 60

Query: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS 120
           CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDD+FPIRDFASRLPASPHSS
Sbjct: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDDFPIRDFASRLPASPHSS 119

[Close]

BLAST of MS008536 vs. NCBI nr
Match: XP_023523424.1 (uncharacterized protein LOC111787637 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 183.0 bits (463), Expect = 1.6e-42
Identity = 93/116 (80.17%), Postives = 101/116 (87.07%), Query Frame = 0


Query: 4   NEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGMCRN 63
           N+Y+   FSFT KLKSSILCFR DHGD  ++EDD+S R+PRSPLKS A+E  ELRGMCRN
Sbjct: 3   NDYH--PFSFTRKLKSSILCFRRDHGDFLENEDDSSPRTPRSPLKSMAAEFPELRGMCRN 62

Query: 64  LVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS 120
           LVARIGRSRRRYHHSADFRYDPSSYALNFEDE  R DDEFPIRDFASRLPASP +S
Sbjct: 63  LVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS 116

[Close]

BLAST of MS008536 vs. NCBI nr
Match: XP_022940408.1 (uncharacterized protein LOC111446022 [Cucurbita moschata] >KAG6608139.1 hypothetical protein SDJN03_01481, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 181.4 bits (459), Expect = 4.5e-42
Identity = 93/119 (78.15%), Postives = 102/119 (85.71%), Query Frame = 0


Query: 1   MLNNEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGM 60
           M+ N+Y+   FSFT KLKSSILCFR DHGD  ++E D+S R+PRSPLKS A+E  ELRGM
Sbjct: 1   MMLNDYH--PFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGM 60

Query: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS 120
           CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDE  R DDEFPIRDFASRLPASP +S
Sbjct: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS 117

[Close]

BLAST of MS008536 vs. NCBI nr
Match: KAG7031776.1 (hypothetical protein SDJN02_05817, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 180.3 bits (456), Expect = 1.0e-41
Identity = 92/116 (79.31%), Postives = 100/116 (86.21%), Query Frame = 0


Query: 4   NEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGMCRN 63
           N+Y+   FSFT KLKSSILCFR DHGD  ++E D+S R+PRSPLKS A+E  ELRGMCRN
Sbjct: 3   NDYH--PFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGMCRN 62

Query: 64  LVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS 120
           LVARIGRSRRRYHHSADFRYDPSSYALNFEDE  R DDEFPIRDFASRLPASP +S
Sbjct: 63  LVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS 116

[Close]

BLAST of MS008536 vs. NCBI nr
Match: XP_022981208.1 (uncharacterized protein LOC111480417 [Cucurbita maxima])

HSP 1 Score: 177.6 bits (449), Expect = 6.5e-41
Identity = 91/119 (76.47%), Postives = 99/119 (83.19%), Query Frame = 0


Query: 1   MLNNEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGM 60
           M+ NEY+   FSFT KLKSSI CFR DH D  + EDD+S R+PRSPLKS A+E  E+RGM
Sbjct: 1   MMLNEYH--PFSFTRKLKSSIRCFRRDHSDFFESEDDSSPRTPRSPLKSMAAEFPEIRGM 60

Query: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS 120
           CRNLVARIGR RRRYHHSADFRYDPSSYALNFEDE  R DDEFPIRDFASRLPASP +S
Sbjct: 61  CRNLVARIGRCRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS 117

[Close]

BLAST of MS008536 vs. ExPASy TrEMBL
Match: A0A6J1CC83 (uncharacterized protein LOC111009386 OS=Momordica charantia OX=3673 GN=LOC111009386 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.2e-59
Identity = 116/119 (97.48%), Postives = 118/119 (99.16%), Query Frame = 0


Query: 1   MLNNEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGM 60
           MLNNEYNSS FSFTHKLKSSILCFRADHGDLGDHED+ASLRSPRSPLKSTASEHTELRGM
Sbjct: 1   MLNNEYNSSPFSFTHKLKSSILCFRADHGDLGDHEDEASLRSPRSPLKSTASEHTELRGM 60

Query: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS 120
           CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDD+FPIRDFASRLPASPHSS
Sbjct: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDDFPIRDFASRLPASPHSS 119

[Close]

BLAST of MS008536 vs. ExPASy TrEMBL
Match: A0A6J1FP70 (uncharacterized protein LOC111446022 OS=Cucurbita moschata OX=3662 GN=LOC111446022 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.2e-42
Identity = 93/119 (78.15%), Postives = 102/119 (85.71%), Query Frame = 0


Query: 1   MLNNEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGM 60
           M+ N+Y+   FSFT KLKSSILCFR DHGD  ++E D+S R+PRSPLKS A+E  ELRGM
Sbjct: 1   MMLNDYH--PFSFTRKLKSSILCFRRDHGDFLENEGDSSPRTPRSPLKSMAAEFPELRGM 60

Query: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS 120
           CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDE  R DDEFPIRDFASRLPASP +S
Sbjct: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS 117

[Close]

BLAST of MS008536 vs. ExPASy TrEMBL
Match: A0A6J1J1H3 (uncharacterized protein LOC111480417 OS=Cucurbita maxima OX=3661 GN=LOC111480417 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 3.2e-41
Identity = 91/119 (76.47%), Postives = 99/119 (83.19%), Query Frame = 0


Query: 1   MLNNEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGM 60
           M+ NEY+   FSFT KLKSSI CFR DH D  + EDD+S R+PRSPLKS A+E  E+RGM
Sbjct: 1   MMLNEYH--PFSFTRKLKSSIRCFRRDHSDFFESEDDSSPRTPRSPLKSMAAEFPEIRGM 60

Query: 61  CRNLVARIGRSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASPHSS 120
           CRNLVARIGR RRRYHHSADFRYDPSSYALNFEDE  R DDEFPIRDFASRLPASP +S
Sbjct: 61  CRNLVARIGRCRRRYHHSADFRYDPSSYALNFEDEQVRDDDEFPIRDFASRLPASPPTS 117

[Close]

BLAST of MS008536 vs. ExPASy TrEMBL
Match: A0A0A0L4E2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652740 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 6.6e-31
Identity = 81/114 (71.05%), Postives = 86/114 (75.44%), Query Frame = 0


Query: 4   NEYNSSSFSFTHKLKSSILCFRADHGDLGDHEDDASLRSPRSPLKSTASEHTELRGMCRN 63
           N YN   FSFTHKLKS I CFR DH D   H++ +S  SP   LKS  S+  ELRGMCR+
Sbjct: 3   NHYN--PFSFTHKLKSPISCFRPDH-DPELHDNPSSPTSP--ALKSMTSDLPELRGMCRS 62

Query: 64  LVARIG-RSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASP 117
           LVARI   SRRRYHHS DFRYDPSSYALNFEDE  RYDDEFPIRDF SRLPASP
Sbjct: 63  LVARISWPSRRRYHHSTDFRYDPSSYALNFEDEQLRYDDEFPIRDFTSRLPASP 111

[Close]

BLAST of MS008536 vs. ExPASy TrEMBL
Match: A0A5A7VBS3 (DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00970 PE=4 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 8.1e-29
Identity = 80/116 (68.97%), Postives = 86/116 (74.14%), Query Frame = 0


Query: 4   NEYNSSSFSFTHKLKSSI-LCFRADH-GDLGDHEDDASLRSPRSPLKSTASEHTELRGMC 63
           N YN   FSFTHKLKSSI  CFR DH  +L ++EDD         LKS  S+  ELRGMC
Sbjct: 3   NHYN--PFSFTHKLKSSIPSCFRPDHDAELHENEDD------NPALKSMTSDLPELRGMC 62

Query: 64  RNLVARIG-RSRRRYHHSADFRYDPSSYALNFEDEHARYDDEFPIRDFASRLPASP 117
           R+LVARI   SRRRYHHSADFRYDPSSYALNFEDE  R D+EFPIRDF SRLPASP
Sbjct: 63  RSLVARISWPSRRRYHHSADFRYDPSSYALNFEDEQVRDDNEFPIRDFTSRLPASP 110

[Close]

BLAST of MS008536 vs. TAIR 10
Match: AT5G11070.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 61.6 bits (148), Expect = 4.9e-10
Identity = 51/125 (40.80%), Postives = 66/125 (52.80%), Query Frame = 0


Query: 15  HKLKSSILCFRADHGDLGDHEDDASL-----RSPRSP---LKSTASEHTELRGMCRNLVA 74
           H+++S I C  A+       E +A +     R+PRSP   LKSTA E  ELR  CR + +
Sbjct: 20  HRMRSPICCIGAN----AVVEPEAMMIGGEQRTPRSPYEWLKSTAQE-LELRDRCRRVKS 79

Query: 75  RIGRSRR-----------RYHHS----ADFRYDPSSYALNFEDEHARYDDEFPIRDFASR 117
           RI  + R            +HHS     DF YDP SYALNFED + R DD+    +F +R
Sbjct: 80  RIKVTCRNNNCAYNNCVHHHHHSQSYPGDFSYDPLSYALNFED-NVRADDDGSFPNFTAR 138

[Close]

BLAST of MS008536 vs. TAIR 10
Match: AT5G35090.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-09
Identity = 49/139 (35.25%), Postives = 67/139 (48.20%), Query Frame = 0


Query: 9   SSFSFTHKLKSSIL---CFRADHGDLGDHEDDASLRSPRSPLKSTASEH----------- 68
           ++ S   KLKS      CFR  +    D+  D    SP +  KST S H           
Sbjct: 7   TNLSLQKKLKSRFCIAGCFRTTNHHHHDNIPDDLPSSPVTTEKSTQSPHGGGMKTKSPRL 66

Query: 69  ----TELRGMCRNLVARI--------GRSRRRYHHSADFRYDPSSYALNFE--DEHARYD 120
               ++    C++L+ R+        G  +    H+ADF YDPSSYALNF+  DE     
Sbjct: 67  TRTLSKSHEKCKSLIHRMGGGVGGVGGHGKHIRRHTADFHYDPSSYALNFDKGDEDDNI- 126

The following BLAST results are available for this feature:

BLAST of MS008536 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138148.1	2.4e-59	97.48	uncharacterized protein LOC111009386 [Momordica charantia]	[more]
XP_023523424.1	1.6e-42	80.17	uncharacterized protein LOC111787637 [Cucurbita pepo subsp. pepo]	[more]
XP_022940408.1	4.5e-42	78.15	uncharacterized protein LOC111446022 [Cucurbita moschata] >KAG6608139.1 hypothet...	[more]
KAG7031776.1	1.0e-41	79.31	hypothetical protein SDJN02_05817, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022981208.1	6.5e-41	76.47	uncharacterized protein LOC111480417 [Cucurbita maxima]	[more]

BLAST of MS008536 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS008536 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CC83	1.2e-59	97.48	uncharacterized protein LOC111009386 OS=Momordica charantia OX=3673 GN=LOC111009...	[more]
A0A6J1FP70	2.2e-42	78.15	uncharacterized protein LOC111446022 OS=Cucurbita moschata OX=3662 GN=LOC1114460...	[more]
A0A6J1J1H3	3.2e-41	76.47	uncharacterized protein LOC111480417 OS=Cucurbita maxima OX=3661 GN=LOC111480417...	[more]
A0A0A0L4E2	6.6e-31	71.05	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G652740 PE=4 SV=1	[more]
A0A5A7VBS3	8.1e-29	68.97	DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00970 PE=...	[more]

BLAST of MS008536 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G11070.1	4.9e-10	40.80	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]
AT5G35090.1	1.1e-09	35.25	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..54
None	No IPR available	PANTHER	PTHR33168:SF61	SUBFAMILY NOT NAMED	coord: 4..118
None	No IPR available	PANTHER	PTHR33168	STRESS INDUCED PROTEIN-RELATED	coord: 4..118

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008536.1	MS008536.1	mRNA