MS008439 (gene) Bitter gourd (TR) v1

Overview


Name	MS008439
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	rust resistance kinase Lr10-like isoform X1
Location	scaffold4: 1430766 .. 1431254 (-)
RNA-Seq Expression	MS008439
Synteny	MS008439

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGGTCAGATTTACCGCTCAGCAACTCCGCGCCTTCACAAGCAACTATTCTACGCGGCTCGGTTCCGGCGGCTTCGGCGATGTTTACAAAGGCCAGTTCCCGAACGGCGTAGAGATCGCAGTGAAGATTCTGAAGAGAAACTCCGAAAAAAAAGCCGAAGAGCAATTCATGGCGGAAATGAGAACAATCGGTAGAACTCGCCATAGAAATCTGGTCAGGCTTTACGGATTCTGTTACGACGAATTCGTGAGCGCGCTGGTTTTGGAGTACATGGAGAACGGATCGCTCGACAGATTCTTGTACGGCAAAAGAAATTACGAAATCGTGTGGGAGAAATTGCAGGAAATTGCGATTGGAACCGCTAGAGGAATCGCTTACCTGCACGAAGAATGCGAGAGAAGAATCATCCACTACGATATTAAACCGGCGAACATTCTATTGGACGCGAATTTCTCGCCAAGAGTCGGCGATTTCGGACTTGCGAATCTC

mRNA sequence

CCGGTCAGATTTACCGCTCAGCAACTCCGCGCCTTCACAAGCAACTATTCTACGCGGCTCGGTTCCGGCGGCTTCGGCGATGTTTACAAAGGCCAGTTCCCGAACGGCGTAGAGATCGCAGTGAAGATTCTGAAGAGAAACTCCGAAAAAAAAGCCGAAGAGCAATTCATGGCGGAAATGAGAACAATCGGTAGAACTCGCCATAGAAATCTGGTCAGGCTTTACGGATTCTGTTACGACGAATTCGTGAGCGCGCTGGTTTTGGAGTACATGGAGAACGGATCGCTCGACAGATTCTTGTACGGCAAAAGAAATTACGAAATCGTGTGGGAGAAATTGCAGGAAATTGCGATTGGAACCGCTAGAGGAATCGCTTACCTGCACGAAGAATGCGAGAGAAGAATCATCCACTACGATATTAAACCGGCGAACATTCTATTGGACGCGAATTTCTCGCCAAGAGTCGGCGATTTCGGACTTGCGAATCTC

Coding sequence (CDS)

CCGGTCAGATTTACCGCTCAGCAACTCCGCGCCTTCACAAGCAACTATTCTACGCGGCTCGGTTCCGGCGGCTTCGGCGATGTTTACAAAGGCCAGTTCCCGAACGGCGTAGAGATCGCAGTGAAGATTCTGAAGAGAAACTCCGAAAAAAAAGCCGAAGAGCAATTCATGGCGGAAATGAGAACAATCGGTAGAACTCGCCATAGAAATCTGGTCAGGCTTTACGGATTCTGTTACGACGAATTCGTGAGCGCGCTGGTTTTGGAGTACATGGAGAACGGATCGCTCGACAGATTCTTGTACGGCAAAAGAAATTACGAAATCGTGTGGGAGAAATTGCAGGAAATTGCGATTGGAACCGCTAGAGGAATCGCTTACCTGCACGAAGAATGCGAGAGAAGAATCATCCACTACGATATTAAACCGGCGAACATTCTATTGGACGCGAATTTCTCGCCAAGAGTCGGCGATTTCGGACTTGCGAATCTC

Protein sequence

PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEMRTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGTARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL

Homology

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BLAST of MS008439 vs. NCBI nr
Match: XP_022136495.1 (rust resistance kinase Lr10-like isoform X2 [Momordica charantia])

HSP 1 Score: 335.5 bits (859), Expect = 2.6e-88
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM
Sbjct: 198 PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 257

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
           RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT
Sbjct: 258 RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 317

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL
Sbjct: 318 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 360

[Close]

BLAST of MS008439 vs. NCBI nr
Match: XP_022136503.1 (rust resistance kinase Lr10-like isoform X3 [Momordica charantia])

HSP 1 Score: 335.5 bits (859), Expect = 2.6e-88
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM
Sbjct: 170 PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 229

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
           RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT
Sbjct: 230 RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 289

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL
Sbjct: 290 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 332

[Close]

BLAST of MS008439 vs. NCBI nr
Match: XP_022136485.1 (rust resistance kinase Lr10-like isoform X1 [Momordica charantia])

HSP 1 Score: 335.5 bits (859), Expect = 2.6e-88
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM
Sbjct: 205 PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 264

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
           RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT
Sbjct: 265 RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 324

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL
Sbjct: 325 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 367

[Close]

BLAST of MS008439 vs. NCBI nr
Match: KAG7024507.1 (Rust resistance kinase Lr10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.8 bits (774), Expect = 1.8e-78
Identity = 142/163 (87.12%), Postives = 155/163 (95.09%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           P+RFTAQQL  FT+NY+TRLGSGGFGDVYKGQFPNG++IAVKILKRNS+KKAE QFMAE+
Sbjct: 106 PIRFTAQQLNVFTNNYATRLGSGGFGDVYKGQFPNGLKIAVKILKRNSDKKAENQFMAEI 165

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
            TIGRTRHRNL+R+YGFCYDEF+SALVLEYMENGSLDRFLYGK   EIVWEKLQEIAIGT
Sbjct: 166 GTIGRTRHRNLLRMYGFCYDEFMSALVLEYMENGSLDRFLYGKTRNEIVWEKLQEIAIGT 225

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEEC RRIIHYDIKPANILLDA+FSP++GDFGLANL
Sbjct: 226 ARGIAYLHEECHRRIIHYDIKPANILLDADFSPKIGDFGLANL 268

[Close]

BLAST of MS008439 vs. NCBI nr
Match: XP_022936982.1 (rust resistance kinase Lr10-like [Cucurbita moschata])

HSP 1 Score: 302.8 bits (774), Expect = 1.8e-78
Identity = 142/163 (87.12%), Postives = 155/163 (95.09%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           P+RFTAQQL  FT+NY+TRLGSGGFGDVYKGQFPNG++IAVKILKRNS+KKAE QFMAE+
Sbjct: 105 PIRFTAQQLNVFTNNYATRLGSGGFGDVYKGQFPNGLKIAVKILKRNSDKKAENQFMAEI 164

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
            TIGRTRHRNL+R+YGFCYDEF+SALVLEYMENGSLDRFLYGK   EIVWEKLQEIAIGT
Sbjct: 165 GTIGRTRHRNLLRMYGFCYDEFMSALVLEYMENGSLDRFLYGKTRNEIVWEKLQEIAIGT 224

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEEC RRIIHYDIKPANILLDA+FSP++GDFGLANL
Sbjct: 225 ARGIAYLHEECHRRIIHYDIKPANILLDADFSPKIGDFGLANL 267

[Close]

BLAST of MS008439 vs. ExPASy Swiss-Prot
Match: C0LGH3 (Probable LRR receptor-like serine/threonine-protein kinase At1g56140 OS=Arabidopsis thaliana OX=3702 GN=At1g56140 PE=2 SV=2)

HSP 1 Score: 162.2 bits (409), Expect = 5.1e-39
Identity = 79/165 (47.88%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNY--STRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMA 60
           P  FT  +L++ T ++  S +LG GGFG VYKG+  +G E+AVK+L   S ++ + QF+A
Sbjct: 678 PYTFTYSELKSATQDFDPSNKLGEGGFGPVYKGKLNDGREVAVKLLSVGS-RQGKGQFVA 737

Query: 61  EMRTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAI 120
           E+  I   +HRNLV+LYG CY+     LV EY+ NGSLD+ L+G++   + W    EI +
Sbjct: 738 EIVAISAVQHRNLVKLYGCCYEGEHRLLVYEYLPNGSLDQALFGEKTLHLDWSTRYEICL 797

Query: 121 GTARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           G ARG+ YLHEE   RI+H D+K +NILLD+   P+V DFGLA L
Sbjct: 798 GVARGLVYLHEEARLRIVHRDVKASNILLDSKLVPKVSDFGLAKL 841

[Close]

BLAST of MS008439 vs. ExPASy Swiss-Prot
Match: Q8RWZ5 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidopsis thaliana OX=3702 GN=SD25 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 6.7e-39
Identity = 78/165 (47.27%), Postives = 113/165 (68.48%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           P+RF  + L++ T+N+S +LG GGFG VY+G  P+G  +AVK L+   + K E  F AE+
Sbjct: 480 PIRFAYKDLQSATNNFSVKLGQGGFGSVYEGTLPDGSRLAVKKLEGIGQGKKE--FRAEV 539

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIV--WEKLQEIAI 120
             IG   H +LVRL GFC +     L  E++  GSL+R+++ K++ +++  W+    IA+
Sbjct: 540 SIIGSIHHLHLVRLRGFCAEGAHRLLAYEFLSKGSLERWIFRKKDGDVLLDWDTRFNIAL 599

Query: 121 GTARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           GTA+G+AYLHE+C+ RI+H DIKP NILLD NF+ +V DFGLA L
Sbjct: 600 GTAKGLAYLHEDCDARIVHCDIKPENILLDDNFNAKVSDFGLAKL 642

[Close]

BLAST of MS008439 vs. ExPASy Swiss-Prot
Match: P93604 (Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 8.7e-39
Identity = 76/167 (45.51%), Postives = 113/167 (67.66%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           P R+T  +++     +  ++G GGFG VYKG+ PNGV +AVK+L+ ++ +   E F+ E+
Sbjct: 326 PTRYTFSEVKKMARRFKEKVGQGGFGSVYKGELPNGVPVAVKMLENSTGE--GESFINEV 385

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRN----YEIVWEKLQEI 120
            TIG   H N+VRL GFC +    AL+ E+M N SL+++++   +      +V EKL +I
Sbjct: 386 ATIGLIHHANIVRLLGFCSEGMRRALIYEFMPNESLEKYIFSDDSNIFQNLLVPEKLLDI 445

Query: 121 AIGTARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           A+G ARG+ YLH+ C +RI+H+DIKP NILLD NF+P++ DFGLA L
Sbjct: 446 ALGIARGMEYLHQGCNQRILHFDIKPHNILLDYNFNPKISDFGLAKL 490

[Close]

BLAST of MS008439 vs. ExPASy Swiss-Prot
Match: Q9FLV4 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabidopsis thaliana OX=3702 GN=At5g24080 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.1e-38
Identity = 83/165 (50.30%), Postives = 105/165 (63.64%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           PV FT + L+  T+N+S  LGSGGFG VYKG       +AVK L R +    E +F+ E+
Sbjct: 517 PVSFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVAVKRLDR-ALSHGEREFITEV 576

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYE--IVWEKLQEIAI 120
            TIG   H NLVRL G+C ++    LV EYM NGSLD++++        + W    EIA+
Sbjct: 577 NTIGSMHHMNLVRLCGYCSEDSHRLLVYEYMINGSLDKWIFSSEQTANLLDWRTRFEIAV 636

Query: 121 GTARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
            TA+GIAY HE+C  RIIH DIKP NILLD NF P+V DFGLA +
Sbjct: 637 ATAQGIAYFHEQCRNRIIHCDIKPENILLDDNFCPKVSDFGLAKM 680

[Close]

BLAST of MS008439 vs. ExPASy Swiss-Prot
Match: D7SFH9 (Protein SUPPRESSOR OF NPR1-1 CONSTITUTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.6 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 5.6e-38
Identity = 74/160 (46.25%), Postives = 105/160 (65.62%), Query Frame = 0


Query: 4   FTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEMRTI 63
           +T  Q++  T +++  +G GGFG VYKG   +G  +AVK+LK    K   E F+ E+ T+
Sbjct: 795 YTYAQVKRITKSFAEVVGRGGFGIVYKGTLSDGRVVAVKVLK--DTKGNGEDFINEVATM 854

Query: 64  GRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGTARG 123
            RT H N+V L GFC +    A++ E++ENGSLD+F+ GK +  + W  L  IA+G A G
Sbjct: 855 SRTSHLNIVSLLGFCSEGSKRAIIYEFLENGSLDKFILGKTSVNMDWTALYRIALGVAHG 914

Query: 124 IAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           + YLH  C+ RI+H+DIKP N+LLD +F P+V DFGLA L
Sbjct: 915 LEYLHHSCKTRIVHFDIKPQNVLLDDSFCPKVSDFGLAKL 952

[Close]

BLAST of MS008439 vs. ExPASy TrEMBL
Match: A0A6J1C423 (rust resistance kinase Lr10-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008185 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.2e-88
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM
Sbjct: 198 PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 257

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
           RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT
Sbjct: 258 RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 317

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL
Sbjct: 318 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 360

[Close]

BLAST of MS008439 vs. ExPASy TrEMBL
Match: A0A6J1C7Q7 (rust resistance kinase Lr10-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111008185 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.2e-88
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM
Sbjct: 170 PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 229

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
           RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT
Sbjct: 230 RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 289

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL
Sbjct: 290 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 332

[Close]

BLAST of MS008439 vs. ExPASy TrEMBL
Match: A0A6J1C413 (rust resistance kinase Lr10-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008185 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.2e-88
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM
Sbjct: 205 PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 264

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
           RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT
Sbjct: 265 RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 324

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL
Sbjct: 325 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 367

[Close]

BLAST of MS008439 vs. ExPASy TrEMBL
Match: A0A6J1F9V0 (rust resistance kinase Lr10-like OS=Cucurbita moschata OX=3662 GN=LOC111443411 PE=3 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 8.9e-79
Identity = 142/163 (87.12%), Postives = 155/163 (95.09%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           P+RFTAQQL  FT+NY+TRLGSGGFGDVYKGQFPNG++IAVKILKRNS+KKAE QFMAE+
Sbjct: 105 PIRFTAQQLNVFTNNYATRLGSGGFGDVYKGQFPNGLKIAVKILKRNSDKKAENQFMAEI 164

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
            TIGRTRHRNL+R+YGFCYDEF+SALVLEYMENGSLDRFLYGK   EIVWEKLQEIAIGT
Sbjct: 165 GTIGRTRHRNLLRMYGFCYDEFMSALVLEYMENGSLDRFLYGKTRNEIVWEKLQEIAIGT 224

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEEC RRIIHYDIKPANILLDA+FSP++GDFGLANL
Sbjct: 225 ARGIAYLHEECHRRIIHYDIKPANILLDADFSPKIGDFGLANL 267

[Close]

BLAST of MS008439 vs. ExPASy TrEMBL
Match: A0A6J1IF18 (rust resistance kinase Lr10-like OS=Cucurbita maxima OX=3661 GN=LOC111476631 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-76
Identity = 138/163 (84.66%), Postives = 152/163 (93.25%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           P+RFTAQQL  FT+NY+TRLGSGGFGDVYKGQFPNG++IAVKILKRNS+KKAE QFMAE+
Sbjct: 105 PIRFTAQQLNVFTNNYATRLGSGGFGDVYKGQFPNGLKIAVKILKRNSDKKAENQFMAEI 164

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGT 120
            TIGRTRHRNL+R+YGFCYDEF+SALVLEYMENGSLDRFLYGK   EI WEKLQEIA GT
Sbjct: 165 GTIGRTRHRNLLRMYGFCYDEFMSALVLEYMENGSLDRFLYGKTRNEIDWEKLQEIATGT 224

Query: 121 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARGIAYLHEEC RRIIHYDIKPANILLD +FSP++GDFGL+NL
Sbjct: 225 ARGIAYLHEECHRRIIHYDIKPANILLDVDFSPKIGDFGLSNL 267

[Close]

BLAST of MS008439 vs. TAIR 10
Match: AT1G56140.1 (Leucine-rich repeat transmembrane protein kinase )

HSP 1 Score: 162.2 bits (409), Expect = 3.6e-40
Identity = 79/165 (47.88%), Postives = 110/165 (66.67%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNY--STRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMA 60
           P  FT  +L++ T ++  S +LG GGFG VYKG+  +G E+AVK+L   S ++ + QF+A
Sbjct: 678 PYTFTYSELKSATQDFDPSNKLGEGGFGPVYKGKLNDGREVAVKLLSVGS-RQGKGQFVA 737

Query: 61  EMRTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAI 120
           E+  I   +HRNLV+LYG CY+     LV EY+ NGSLD+ L+G++   + W    EI +
Sbjct: 738 EIVAISAVQHRNLVKLYGCCYEGEHRLLVYEYLPNGSLDQALFGEKTLHLDWSTRYEICL 797

Query: 121 GTARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           G ARG+ YLHEE   RI+H D+K +NILLD+   P+V DFGLA L
Sbjct: 798 GVARGLVYLHEEARLRIVHRDVKASNILLDSKLVPKVSDFGLAKL 841

[Close]

BLAST of MS008439 vs. TAIR 10
Match: AT4G32300.1 (S-domain-2 5 )

HSP 1 Score: 161.8 bits (408), Expect = 4.7e-40
Identity = 78/165 (47.27%), Postives = 113/165 (68.48%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           P+RF  + L++ T+N+S +LG GGFG VY+G  P+G  +AVK L+   + K E  F AE+
Sbjct: 480 PIRFAYKDLQSATNNFSVKLGQGGFGSVYEGTLPDGSRLAVKKLEGIGQGKKE--FRAEV 539

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIV--WEKLQEIAI 120
             IG   H +LVRL GFC +     L  E++  GSL+R+++ K++ +++  W+    IA+
Sbjct: 540 SIIGSIHHLHLVRLRGFCAEGAHRLLAYEFLSKGSLERWIFRKKDGDVLLDWDTRFNIAL 599

Query: 121 GTARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           GTA+G+AYLHE+C+ RI+H DIKP NILLD NF+ +V DFGLA L
Sbjct: 600 GTAKGLAYLHEDCDARIVHCDIKPENILLDDNFNAKVSDFGLAKL 642

[Close]

BLAST of MS008439 vs. TAIR 10
Match: AT5G24080.1 (Protein kinase superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 8.1e-40
Identity = 83/165 (50.30%), Postives = 105/165 (63.64%), Query Frame = 0


Query: 1   PVRFTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEM 60
           PV FT + L+  T+N+S  LGSGGFG VYKG       +AVK L R +    E +F+ E+
Sbjct: 115 PVSFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVAVKRLDR-ALSHGEREFITEV 174

Query: 61  RTIGRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYE--IVWEKLQEIAI 120
            TIG   H NLVRL G+C ++    LV EYM NGSLD++++        + W    EIA+
Sbjct: 175 NTIGSMHHMNLVRLCGYCSEDSHRLLVYEYMINGSLDKWIFSSEQTANLLDWRTRFEIAV 234

Query: 121 GTARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
            TA+GIAY HE+C  RIIH DIKP NILLD NF P+V DFGLA +
Sbjct: 235 ATAQGIAYFHEQCRNRIIHCDIKPENILLDDNFCPKVSDFGLAKM 278

[Close]

BLAST of MS008439 vs. TAIR 10
Match: AT1G66980.1 (suppressor of npr1-1 constitutive 4 )

HSP 1 Score: 158.7 bits (400), Expect = 4.0e-39
Identity = 74/160 (46.25%), Postives = 105/160 (65.62%), Query Frame = 0


Query: 4   FTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEMRTI 63
           +T  Q++  T +++  +G GGFG VYKG   +G  +AVK+LK    K   E F+ E+ T+
Sbjct: 795 YTYAQVKRITKSFAEVVGRGGFGIVYKGTLSDGRVVAVKVLK--DTKGNGEDFINEVATM 854

Query: 64  GRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIVWEKLQEIAIGTARG 123
            RT H N+V L GFC +    A++ E++ENGSLD+F+ GK +  + W  L  IA+G A G
Sbjct: 855 SRTSHLNIVSLLGFCSEGSKRAIIYEFLENGSLDKFILGKTSVNMDWTALYRIALGVAHG 914

Query: 124 IAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           + YLH  C+ RI+H+DIKP N+LLD +F P+V DFGLA L
Sbjct: 915 LEYLHHSCKTRIVHFDIKPQNVLLDDSFCPKVSDFGLAKL 952

[Close]

BLAST of MS008439 vs. TAIR 10
Match: AT2G19130.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 157.9 bits (398), Expect = 6.8e-39
Identity = 83/163 (50.92%), Postives = 109/163 (66.87%), Query Frame = 0


Query: 4   FTAQQLRAFTSNYSTRLGSGGFGDVYKGQFPNGVEIAVKILKRNSEKKAEEQFMAEMRTI 63
           F+ ++L+  T N+S +LG GGFG V+KG  P+  +IAVK L+  S+   E+QF  E+ TI
Sbjct: 483 FSYRELQNATKNFSDKLGGGGFGSVFKGALPDSSDIAVKRLEGISQ--GEKQFRTEVVTI 542

Query: 64  GRTRHRNLVRLYGFCYDEFVSALVLEYMENGSLDRFLYGKRNYEIV---WEKLQEIAIGT 123
           G  +H NLVRL GFC +     LV +YM NGSLD  L+  +  E +   W+   +IA+GT
Sbjct: 543 GTIQHVNLVRLRGFCSEGSKKLLVYDYMPNGSLDSHLFLNQVEEKIVLGWKLRFQIALGT 602

Query: 124 ARGIAYLHEECERRIIHYDIKPANILLDANFSPRVGDFGLANL 164
           ARG+AYLH+EC   IIH DIKP NILLD+ F P+V DFGLA L
Sbjct: 603 ARGLAYLHDECRDCIIHCDIKPENILLDSQFCPKVADFGLAKL 643

The following BLAST results are available for this feature:

BLAST of MS008439 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136495.1	2.6e-88	100.00	rust resistance kinase Lr10-like isoform X2 [Momordica charantia]	[more]
XP_022136503.1	2.6e-88	100.00	rust resistance kinase Lr10-like isoform X3 [Momordica charantia]	[more]
XP_022136485.1	2.6e-88	100.00	rust resistance kinase Lr10-like isoform X1 [Momordica charantia]	[more]
KAG7024507.1	1.8e-78	87.12	Rust resistance kinase Lr10, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_022936982.1	1.8e-78	87.12	rust resistance kinase Lr10-like [Cucurbita moschata]	[more]

BLAST of MS008439 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0LGH3	5.1e-39	47.88	Probable LRR receptor-like serine/threonine-protein kinase At1g56140 OS=Arabidop...	[more]
Q8RWZ5	6.7e-39	47.27	G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidops...	[more]
P93604	8.7e-39	45.51	Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1	[more]
Q9FLV4	1.1e-38	50.30	G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabi...	[more]
D7SFH9	5.6e-38	46.25	Protein SUPPRESSOR OF NPR1-1 CONSTITUTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=L...	[more]

BLAST of MS008439 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C423	1.2e-88	100.00	rust resistance kinase Lr10-like isoform X2 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1C7Q7	1.2e-88	100.00	rust resistance kinase Lr10-like isoform X3 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1C413	1.2e-88	100.00	rust resistance kinase Lr10-like isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1F9V0	8.9e-79	87.12	rust resistance kinase Lr10-like OS=Cucurbita moschata OX=3662 GN=LOC111443411 P...	[more]
A0A6J1IF18	1.4e-76	84.66	rust resistance kinase Lr10-like OS=Cucurbita maxima OX=3661 GN=LOC111476631 PE=...	[more]

BLAST of MS008439 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56140.1	3.6e-40	47.88	Leucine-rich repeat transmembrane protein kinase	[more]
AT4G32300.1	4.7e-40	47.27	S-domain-2 5	[more]
AT5G24080.1	8.1e-40	50.30	Protein kinase superfamily protein	[more]
AT1G66980.1	4.0e-39	46.25	suppressor of npr1-1 constitutive 4	[more]
AT2G19130.1	6.8e-39	50.92	S-locus lectin protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 14..163 e-value: 5.2E-6 score: -50.1
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 17..162 e-value: 7.3E-34 score: 117.3
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 14..163 score: 29.559153
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 1..90 e-value: 8.0E-28 score: 98.6
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 91..163 e-value: 1.2E-22 score: 82.2
None	No IPR available	PIRSR	PIRSR037921-1	PIRSR037921-1	coord: 20..161 e-value: 2.2E-20 score: 71.1
None	No IPR available	PANTHER	PTHR27009	RUST RESISTANCE KINASE LR10-RELATED	coord: 1..163
None	No IPR available	PANTHER	PTHR27009:SF80	RECEPTOR SERINE/THREONINE KINASE	coord: 1..163
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 135..147
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 20..42
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 3..162

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008439.1	MS008439.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity