MS008434 (gene) Bitter gourd (TR) v1

BLAST of MS008434 vs. NCBI nr
Match: XP_022136854.1 (probable E3 ubiquitin-protein ligase RHG1A [Momordica charantia])

HSP 1 Score: 944.1 bits (2439), Expect = 4.9e-271
Identity = 498/538 (92.57%), Postives = 499/538 (92.75%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS 62
           MDEYSSRRVHVGIVSRKG GVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS
Sbjct: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS 60

Query: 63  SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF 122
           SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF
Sbjct: 61  SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF 120

Query: 123 ISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS 182
           ISPPGMFHT LHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS
Sbjct: 121 ISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS 180

Query: 183 RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSSSR 242
           RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYD ENSSSSR
Sbjct: 181 RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSSR 240

Query: 243 GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQGIS 302
           GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASH YQGIS
Sbjct: 241 GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGIS 300

Query: 303 GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIGQTT 362
           GDRSLQDSLMTSWMPQSNISGSSHTSSSLQS SESHSRHHMTYPEHEDYSQSLLDIGQTT
Sbjct: 301 GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQTT 360

Query: 363 AAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEEL-------------------- 422
           AAEGGISSTSTNLNSFRCRN+DGIAEVLLALERIEQEEEL                    
Sbjct: 361 AAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIH 420

Query: 423 -------------TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTSSVE 482
                        TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGG LTSSVE
Sbjct: 421 DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVE 480

Query: 483 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 508
           DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK
Sbjct: 481 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 538

BLAST of MS008434 vs. NCBI nr
Match: XP_023524149.1 (uncharacterized protein LOC111788150 [Cucurbita pepo subsp. pepo] >XP_023524150.1 uncharacterized protein LOC111788150 [Cucurbita pepo subsp. pepo] >XP_023524151.1 uncharacterized protein LOC111788150 [Cucurbita pepo subsp. pepo] >XP_023524152.1 uncharacterized protein LOC111788150 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 718.4 bits (1853), Expect = 4.4e-203
Identity = 394/543 (72.56%), Postives = 434/543 (79.93%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 62
           MDEYSS+RVH+GIVSRKG GVVLRDN+KNRD SN +G+RLGCTGR+C++SG +VGCS+K 
Sbjct: 1   MDEYSSKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRVCTTSGVQVGCSNKT 60

Query: 63  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 122
           SSKRPFRSSSGKE++GSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVLGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 123 EFISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 182
           EFISPP +FHTGL VKSESS+PAD +LMERGS STD+NT+S+RNS+QR+ VDNQD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPADTLLMERGSCSTDANTESKRNSVQRFEVDNQDSHASM 180

Query: 183 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSS 242
           + RSLCQ  NGG SRY SR+L C+SVSEIIP D S  E+NLVRRKNVTKKMIYD ENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRSLPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 243 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQG 302
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA H YQ 
Sbjct: 241 SRGKKVGGTSRGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 303 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIG 362
            S + S QDSL M SWMPQSNIS SS+  SSLQS SESHSRH MTYPE EDYSQS+ DIG
Sbjct: 301 NSDNLSSQDSLAMNSWMPQSNISSSSNAFSSLQSSSESHSRHRMTYPEPEDYSQSIHDIG 360

Query: 363 QTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEEL----------------- 422
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEEL                 
Sbjct: 361 RATPTEADISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 423 ----------------TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTS 482
                           TYEELLDLEERMGTVSTALSEEALTECL+ SI+QSKP G A T 
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLDTSIYQSKPGGRAATG 480

Query: 483 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 508
           S+E+LSD+KCCICQEEY+ GDEVGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDEVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MS008434 vs. NCBI nr
Match: XP_022940547.1 (uncharacterized protein LOC111446115 [Cucurbita moschata] >XP_022940548.1 uncharacterized protein LOC111446115 [Cucurbita moschata] >XP_022940549.1 uncharacterized protein LOC111446115 [Cucurbita moschata])

HSP 1 Score: 716.5 bits (1848), Expect = 1.7e-202
Identity = 395/543 (72.74%), Postives = 431/543 (79.37%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 62
           MDEYSS+RVH+GIVSRKG GVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYSSKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 63  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 122
           SSKRPFRSSSGKE+VGSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVVGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 123 EFISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 182
           EFISPP +FHTGL VKSESS+PA+  LMERGS STD+NT+S+RNS+QR+ VDNQD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPANTSLMERGSCSTDANTESKRNSVQRFEVDNQDSHASM 180

Query: 183 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSS 242
           + RSLCQ  NGG SRY SRN  C+SVSEIIP D S  E+NLVRRKNVTKKMIYD ENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRNFPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 243 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQG 302
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA H YQ 
Sbjct: 241 SRGKKVGGTSRGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 303 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIG 362
            S + S QDSL M SWMPQSNIS SS+  SSLQS SESHSRH M YPE EDYSQS+ DIG
Sbjct: 301 NSDNLSSQDSLAMNSWMPQSNISSSSNAFSSLQSSSESHSRHRMIYPEPEDYSQSIHDIG 360

Query: 363 QTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEEL----------------- 422
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEEL                 
Sbjct: 361 RATPTEADISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 423 ----------------TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTS 482
                           TYEELLDLEERMGTVSTALSEEALTECLN SI+QSKP G A T 
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNTSIYQSKPGGRAATG 480

Query: 483 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 508
           S+E+LSD+KCCICQEEY+ GDEVGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDEVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MS008434 vs. NCBI nr
Match: KAG7037563.1 (putative E3 ubiquitin-protein ligase HIP1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 707.6 bits (1825), Expect = 7.7e-200
Identity = 391/542 (72.14%), Postives = 427/542 (78.78%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 62
           MDEYSS+RVH+GIVSRKG GVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYSSKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 63  SSKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELE 122
           SSKRPFRSSSGKE+VGS   SS+I N RKSFP+PF KLSSKLETDSSE+ SIQD+LEELE
Sbjct: 61  SSKRPFRSSSGKEVVGS---SSAIINLRKSFPEPFAKLSSKLETDSSENDSIQDDLEELE 120

Query: 123 FISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMR 182
           FISPP +FHTGL VKSESS+PA+  LMERGS STD+NT+S+RNS+QR+ VDNQD+  SM+
Sbjct: 121 FISPPVLFHTGLQVKSESSLPANTSLMERGSCSTDANTESKRNSVQRFEVDNQDSHASMQ 180

Query: 183 SRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSSS 242
            RSLCQ  NGG SRY SRN  C+SVSEIIP   S  E+NLVRRKNVTKKMIYD ENSS S
Sbjct: 181 PRSLCQTSNGGNSRYSSRNFPCDSVSEIIPSYGSLLERNLVRRKNVTKKMIYDGENSSHS 240

Query: 243 RGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQGI 302
           RGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA H YQ  
Sbjct: 241 RGKKVGGTSRGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQSN 300

Query: 303 SGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIGQ 362
           S + S QDSL M SWMPQSNIS SS+  SSLQS SESHSRH MTYPE EDYSQS+ DIG+
Sbjct: 301 SDNLSSQDSLAMNSWMPQSNISSSSNAFSSLQSSSESHSRHRMTYPEPEDYSQSIHDIGR 360

Query: 363 TTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEEL------------------ 422
            T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEEL                  
Sbjct: 361 ATPTEADISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSLN 420

Query: 423 ---------------TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTSS 482
                          TYEELLDLEERMGTVSTALSEEALTECLN SI+QSKP G A T S
Sbjct: 421 IYDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNTSIYQSKPGGRAATGS 480

Query: 483 VEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLHP 508
           +E+LSD+KCCICQEEY+ GDEVGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLHP
Sbjct: 481 IEELSDIKCCICQEEYLIGDEVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLHP 537

BLAST of MS008434 vs. NCBI nr
Match: XP_022981972.1 (uncharacterized protein LOC111480963 [Cucurbita maxima] >XP_022981973.1 uncharacterized protein LOC111480963 [Cucurbita maxima] >XP_022981974.1 uncharacterized protein LOC111480963 [Cucurbita maxima] >XP_022981975.1 uncharacterized protein LOC111480963 [Cucurbita maxima] >XP_022981976.1 uncharacterized protein LOC111480963 [Cucurbita maxima])

HSP 1 Score: 706.4 bits (1822), Expect = 1.7e-199
Identity = 389/543 (71.64%), Postives = 428/543 (78.82%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 62
           MDEY+S+RVH+GIVSRKG GVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYASKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 63  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 122
           SSKRPFRSSSGKE+VGSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVVGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 123 EFISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 182
           EFISPP +FHTGL VKSESS+PAD +LMERG  STD+NT+S+RNS+QR+ VD QD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPADTLLMERGGCSTDANTESKRNSVQRFEVDCQDSHASM 180

Query: 183 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSS 242
           + RSLCQ  NGG SRY SRNL C+SVSEIIP D S  E+NLVRRKNVTKKMIYD ENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRNLPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 243 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQG 302
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA H YQ 
Sbjct: 241 SRGKKVGGTSQGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 303 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIG 362
            S + SL+DSL + SWMPQSNIS SS+  SSLQS SESHSRH MTYPE EDYSQS+ DIG
Sbjct: 301 NSDNLSLEDSLALNSWMPQSNISSSSNAFSSLQSSSESHSRHRMTYPEPEDYSQSIHDIG 360

Query: 363 QTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEEL----------------- 422
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEEL                 
Sbjct: 361 RATPTEVDISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 423 ----------------TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTS 482
                           TYEELLDLEERMGTVSTALSEE LTECLN SI+Q KP G A   
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEVLTECLNTSIYQPKPGGRAAMG 480

Query: 483 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 508
           S+E+LSD+KCCICQEEY+ GD VGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDNVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MS008434 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 6.1e-22
Identity = 54/101 (53.47%), Postives = 70/101 (69.31%), Query Frame = 0

Query: 400 EELTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTSSVEDLSDVKCCIC 459
           + ++YEELL LEER+G VST LSEE +T+ L +  F S      L +SVE   +  CCIC
Sbjct: 571 DNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSS----WRLEASVE---EEPCCIC 630

Query: 460 QEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA 501
           QEEYV GD++G L C H +HV C++QWL +KN CPICK +A
Sbjct: 631 QEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNTA 664

BLAST of MS008434 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.1e-18
Identity = 44/100 (44.00%), Postives = 67/100 (67.00%), Query Frame = 0

Query: 400 EELTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTSSVEDLSDVKCCIC 459
           + ++YEELL LEER+G V T ++EE ++  L +  ++S       T S +D     CC+C
Sbjct: 589 DNMSYEELLALEERIGDVCTGVNEETISNRLKQRKYKSN------TKSPQDAE--PCCVC 648

Query: 460 QEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTS 500
           QEEY  G+++G L C H++H +CI++WL+ KN CPICKT+
Sbjct: 649 QEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNLCPICKTT 680

BLAST of MS008434 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.4e-18
Identity = 71/207 (34.30%), Postives = 104/207 (50.24%), Query Frame = 0

Query: 322 SGSSHTSSSLQSPSESHSRHHMT-------YPEHEDYSQSLLDIGQTTAAEGG-----IS 381
           +G S +S+ +  PS S+SR H +         E ++    L  IG++ AA+G      IS
Sbjct: 499 AGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNELLHLRHIGRSLAADGNGRNQIIS 558

Query: 382 STSTNLNSFRCRNVDGIAEVLLALERIEQ---------------EEELTYEELLDLEERM 441
                L++ R      + + ++    I Q                + ++YEELL L ER+
Sbjct: 559 EIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELLALGERI 618

Query: 442 GTVSTALSEEALTECLNR-SIFQSKPQGGALTSSVEDLSDVKCCICQEEYVSGDEVGRLH 501
           G VST LSEE + + + +     S P    L  ++E      CCICQEEYV GD +G L 
Sbjct: 619 GDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIE-----PCCICQEEYVEGDNLGTLK 678

BLAST of MS008434 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 3.1e-18
Identity = 46/101 (45.54%), Postives = 64/101 (63.37%), Query Frame = 0

Query: 400 EELTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTSSVEDLSDVKCCIC 459
           + +TYEELL LEE++G V+T L++  + E L  S+F       +  SS   + +  C IC
Sbjct: 415 DSMTYEELLALEEQIGDVNTGLAKSYIVEKLKTSLFVPGSSCMSNKSSESSMENDACIIC 474

Query: 460 QEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA 501
           QEEY   + +G L C H+YH  CI+QWL +KN CPICKT+A
Sbjct: 475 QEEYQVKECIGTLDCGHRYHEDCIKQWLMVKNLCPICKTTA 515

BLAST of MS008434 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 4.1e-18
Identity = 71/216 (32.87%), Postives = 102/216 (47.22%), Query Frame = 0

Query: 322 SGSSHTSSSLQSPSESHSRHHMTYP-------EHEDYSQSLLDIGQTTAAEGGISSTSTN 381
           +G S +S+   +PS S SR H +         E ++    L  +G++ AA+         
Sbjct: 465 TGPSVSSNEAAAPSGSSSRSHRSRQRRSGLLLERQNDHLHLRHLGRSLAAD--------- 524

Query: 382 LNSFRCRNVDGIAEVLLALERIE------------------------------QEEELTY 441
            N  R R +  I +VL A+ R E                                + ++Y
Sbjct: 525 -NDGRNRLISEIRQVLSAMRRGENLRFEDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSY 584

Query: 442 EELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTSSVEDLSDVKCCICQEEYV 501
           EELL L ER+G VST LSEE + + + +    S   G     S +D+    CC+CQEEY 
Sbjct: 585 EELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAG-----SHQDME--PCCVCQEEYA 644

BLAST of MS008434 vs. ExPASy TrEMBL
Match: A0A6J1C5H9 (probable E3 ubiquitin-protein ligase RHG1A OS=Momordica charantia OX=3673 GN=LOC111008445 PE=4 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 2.4e-271
Identity = 498/538 (92.57%), Postives = 499/538 (92.75%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS 62
           MDEYSSRRVHVGIVSRKG GVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS
Sbjct: 1   MDEYSSRRVHVGIVSRKGPGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAEVGCSSKAS 60

Query: 63  SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF 122
           SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF
Sbjct: 61  SKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELEF 120

Query: 123 ISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS 182
           ISPPGMFHT LHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS
Sbjct: 121 ISPPGMFHTVLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMRS 180

Query: 183 RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSSSR 242
           RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYD ENSSSSR
Sbjct: 181 RSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDGENSSSSR 240

Query: 243 GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQGIS 302
           GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASH YQGIS
Sbjct: 241 GKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHSYQGIS 300

Query: 303 GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIGQTT 362
           GDRSLQDSLMTSWMPQSNISGSSHTSSSLQS SESHSRHHMTYPEHEDYSQSLLDIGQTT
Sbjct: 301 GDRSLQDSLMTSWMPQSNISGSSHTSSSLQSRSESHSRHHMTYPEHEDYSQSLLDIGQTT 360

Query: 363 AAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEEL-------------------- 422
           AAEGGISSTSTNLNSFRCRN+DGIAEVLLALERIEQEEEL                    
Sbjct: 361 AAEGGISSTSTNLNSFRCRNLDGIAEVLLALERIEQEEELTYEQAILLETSLFLSSLNIH 420

Query: 423 -------------TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTSSVE 482
                        TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGG LTSSVE
Sbjct: 421 DQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGELTSSVE 480

Query: 483 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 508
           DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK
Sbjct: 481 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 538

BLAST of MS008434 vs. ExPASy TrEMBL
Match: A0A6J1FKJ9 (uncharacterized protein LOC111446115 OS=Cucurbita moschata OX=3662 GN=LOC111446115 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 8.1e-203
Identity = 395/543 (72.74%), Postives = 431/543 (79.37%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 62
           MDEYSS+RVH+GIVSRKG GVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYSSKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 63  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 122
           SSKRPFRSSSGKE+VGSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVVGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 123 EFISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 182
           EFISPP +FHTGL VKSESS+PA+  LMERGS STD+NT+S+RNS+QR+ VDNQD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPANTSLMERGSCSTDANTESKRNSVQRFEVDNQDSHASM 180

Query: 183 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSS 242
           + RSLCQ  NGG SRY SRN  C+SVSEIIP D S  E+NLVRRKNVTKKMIYD ENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRNFPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 243 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQG 302
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA H YQ 
Sbjct: 241 SRGKKVGGTSRGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 303 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIG 362
            S + S QDSL M SWMPQSNIS SS+  SSLQS SESHSRH M YPE EDYSQS+ DIG
Sbjct: 301 NSDNLSSQDSLAMNSWMPQSNISSSSNAFSSLQSSSESHSRHRMIYPEPEDYSQSIHDIG 360

Query: 363 QTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEEL----------------- 422
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEEL                 
Sbjct: 361 RATPTEADISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 423 ----------------TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTS 482
                           TYEELLDLEERMGTVSTALSEEALTECLN SI+QSKP G A T 
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEALTECLNTSIYQSKPGGRAATG 480

Query: 483 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 508
           S+E+LSD+KCCICQEEY+ GDEVGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDEVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MS008434 vs. ExPASy TrEMBL
Match: A0A6J1J3B4 (uncharacterized protein LOC111480963 OS=Cucurbita maxima OX=3661 GN=LOC111480963 PE=4 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 8.3e-200
Identity = 389/543 (71.64%), Postives = 428/543 (78.82%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 62
           MDEY+S+RVH+GIVSRKG GVVLRDN+KNRD SN +G+RLGCTGRIC++SG +VGCS+K 
Sbjct: 1   MDEYASKRVHMGIVSRKGPGVVLRDNVKNRDQSNRYGNRLGCTGRICTTSGVQVGCSNKT 60

Query: 63  SSKRPFRSSSGKEIVGSSSRSSS-IRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEEL 122
           SSKRPFRSSSGKE+VGSSS+SSS IRN RKSFP+PF KLSSKLETDSSE+ SIQD+LEEL
Sbjct: 61  SSKRPFRSSSGKEVVGSSSKSSSAIRNLRKSFPEPFAKLSSKLETDSSENDSIQDDLEEL 120

Query: 123 EFISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSM 182
           EFISPP +FHTGL VKSESS+PAD +LMERG  STD+NT+S+RNS+QR+ VD QD+  SM
Sbjct: 121 EFISPPVLFHTGLQVKSESSLPADTLLMERGGCSTDANTESKRNSVQRFEVDCQDSHASM 180

Query: 183 RSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSS 242
           + RSLCQ  NGG SRY SRNL C+SVSEIIP D S  E+NLVRRKNVTKKMIYD ENSS 
Sbjct: 181 QPRSLCQTSNGGNSRYSSRNLPCDSVSEIIPSDGSLLERNLVRRKNVTKKMIYDGENSSH 240

Query: 243 SRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQG 302
           SRGKKV GTS GQNC  R GI ISDQR GRN+FHRQNVLSS GTRSL S C GA H YQ 
Sbjct: 241 SRGKKVGGTSQGQNCVSRHGIFISDQRPGRNVFHRQNVLSSYGTRSLTSVCSGARHSYQS 300

Query: 303 ISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIG 362
            S + SL+DSL + SWMPQSNIS SS+  SSLQS SESHSRH MTYPE EDYSQS+ DIG
Sbjct: 301 NSDNLSLEDSLALNSWMPQSNISSSSNAFSSLQSSSESHSRHRMTYPEPEDYSQSIHDIG 360

Query: 363 QTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEEL----------------- 422
           + T  E  ISST TNLNSFRC N  GIAEVLLALERIEQEEEL                 
Sbjct: 361 RATPTEVDISSTLTNLNSFRCHN--GIAEVLLALERIEQEEELTYEQAILLETSMFLNSL 420

Query: 423 ----------------TYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTS 482
                           TYEELLDLEERMGTVSTALSEE LTECLN SI+Q KP G A   
Sbjct: 421 NINDQHRDMRLDIDNMTYEELLDLEERMGTVSTALSEEVLTECLNTSIYQPKPGGRAAMG 480

Query: 483 SVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSA--HQLH 508
           S+E+LSD+KCCICQEEY+ GD VGRL CEHKYHV CIQQWLRLKNWCPICK SA   QLH
Sbjct: 481 SIEELSDIKCCICQEEYLIGDNVGRLQCEHKYHVACIQQWLRLKNWCPICKASAASQQLH 540

BLAST of MS008434 vs. ExPASy TrEMBL
Match: A0A0A0LJG2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G881840 PE=4 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.5e-196
Identity = 377/509 (74.07%), Postives = 418/509 (82.12%), Query Frame = 0

Query: 1   FLMDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSNH-GSRLGCTGRICSSSGAEVGCSS 60
           FLMD YSS+RVHVGIVSRKG GVVLRDNMK+RD SN   +R GC GRICSSSGA+VGCSS
Sbjct: 58  FLMDGYSSKRVHVGIVSRKGPGVVLRDNMKHRDQSNKCSNRPGCNGRICSSSGAQVGCSS 117

Query: 61  KASSKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEE 120
           K SSKRPF SSSGKE  GS   SS I+N RKSFPDPFGKLSSKLETDSSE+ SIQD+LEE
Sbjct: 118 KFSSKRPFHSSSGKEGFGS---SSGIQNLRKSFPDPFGKLSSKLETDSSENDSIQDDLEE 177

Query: 121 LEFISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTS 180
           LEFISP G+FHTGLH KSES V A+A+LMERGS STDS+  S+R SIQ Y VDN+D   S
Sbjct: 178 LEFISPHGLFHTGLHAKSESPVVANAMLMERGSCSTDSSNISKRVSIQSYEVDNEDPHAS 237

Query: 181 MRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSS 240
           MR RSLCQ  NG +SRY SRNL C+SVSE+IP ++S  E NLVRRKNV KKM +D ENSS
Sbjct: 238 MRPRSLCQKSNGSSSRYNSRNLTCDSVSEVIPSNNSLLESNLVRRKNVMKKMTFDGENSS 297

Query: 241 SSRGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQ 300
           SSRGKKVSGTS GQ C YR GISISDQ  GRN+FHR+N+LSS GTRSL S C G  H YQ
Sbjct: 298 SSRGKKVSGTSRGQKCIYRHGISISDQIPGRNVFHRKNILSSHGTRSLTSVCSGGRHSYQ 357

Query: 301 GISGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDI 360
           GIS + SLQDSL  TSW PQ+N+S SS+ S+SLQ  SESHSR H T P+ E YSQ L DI
Sbjct: 358 GISDNLSLQDSLATTSWRPQANVSSSSNASTSLQLSSESHSRQHTTSPKPEGYSQRLHDI 417

Query: 361 GQTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEELTYEELLDLEERMGTVS 420
            Q T+AE G+SSTSTN + FRC NVDGIAEVLLALERIEQEEELTYE+LLDLEE+MGTVS
Sbjct: 418 RQATSAEVGLSSTSTNASRFRCHNVDGIAEVLLALERIEQEEELTYEQLLDLEEKMGTVS 477

Query: 421 TALSEEALTECLNRSIFQSKPQGGALTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKY 480
           TALSEEALT+CLNRSI+QSKP+G      + +LSDVKCCICQEEYVSGDEVGRL CEH+Y
Sbjct: 478 TALSEEALTKCLNRSIYQSKPKGVTAMDPIGELSDVKCCICQEEYVSGDEVGRLQCEHQY 537

Query: 481 HVKCIQQWLRLKNWCPICKTSAHQLHPFK 508
           HV CIQQWLRLKNWCPICK+SA   HPF+
Sbjct: 538 HVACIQQWLRLKNWCPICKSSAATSHPFR 563

BLAST of MS008434 vs. ExPASy TrEMBL
Match: A0A6J1F8U9 (uncharacterized RING finger protein P4H10.07-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443372 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.8e-192
Identity = 380/538 (70.63%), Postives = 425/538 (79.00%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN-HGSRLGCTGRICSSSGAEVGCSSKA 62
           MDEY S+RVHVGIVSRKG GVVLRDNMK RD SN H  RLGCTGRICSSS A+VG S+KA
Sbjct: 1   MDEYCSKRVHVGIVSRKGPGVVLRDNMKQRDQSNRHSDRLGCTGRICSSSDAQVGRSNKA 60

Query: 63  SSKRPFRSSSGKEIVGSSSRSSSIRNFRKSFPDPFGKLSSKLETDSSEDGSIQDELEELE 122
           SSKR FRSSSGKE+VGS   SS+IRNFR SFP+PFGKLSSKLETDSS++ SIQDELEE+E
Sbjct: 61  SSKRLFRSSSGKEVVGS---SSAIRNFRNSFPEPFGKLSSKLETDSSDNDSIQDELEEME 120

Query: 123 FISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDTSTSMR 182
           F SPPG+ HTGLHVKSE SVPADA+LMERGS S +SN KS+RN+IQRY VDNQ+T  SM 
Sbjct: 121 FTSPPGLLHTGLHVKSERSVPADAMLMERGSCSMNSNNKSKRNTIQRYKVDNQETHASMW 180

Query: 183 SRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSENSSSS 242
            RSLCQ  NGG+SR+ SRNL C+SVSEI+  DSS  +QNLVRRKNVTKKMIYD ENSS+S
Sbjct: 181 PRSLCQISNGGSSRFDSRNLACDSVSEIVSSDSSLLDQNLVRRKNVTKKMIYDGENSSNS 240

Query: 243 RGKKVSGTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACDGASHYYQGI 302
           R K VSGTS GQ C YR GISISDQR GRN+FH+QNVLSS GTRS  S C GA H  QGI
Sbjct: 241 REKIVSGTSRGQKCSYRHGISISDQRPGRNVFHKQNVLSSHGTRSWTSGCGGARH-SQGI 300

Query: 303 SGDRSLQDSL-MTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYSQSLLDIGQ 362
           S + S+ DSL MTSW P++++S SS+ SSSLQ  SESHSRH MTYPE E YS+SL     
Sbjct: 301 SDNLSVHDSLTMTSWTPRTSVSSSSNASSSLQLSSESHSRHRMTYPEPEGYSRSL----- 360

Query: 363 TTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEE-------------------- 422
             AAE GIS+TSTN+NSFRCRNV+GIAEVLLALERIE+EE                    
Sbjct: 361 -HAAEAGISNTSTNVNSFRCRNVNGIAEVLLALERIEREERTYEAILLETSTFLNRLNIY 420

Query: 423 -----------ELTYEELLDLEERMGTVSTALSEEALTECLNRSIFQSKPQGGALTSSVE 482
                       +TYE+LLDLEE MGTVSTALSE+ LTECL+RSI+QSKP+GG  T SVE
Sbjct: 421 DQHRDMRLDIDNMTYEQLLDLEEGMGTVSTALSEKELTECLHRSIYQSKPEGGTATGSVE 480

Query: 483 DLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKTSAHQLHPFK 508
           DLSDVKCCICQEEY SGDEVGRL CEHKYHV CIQQWLR+KNWCPICK++A  L PF+
Sbjct: 481 DLSDVKCCICQEEYASGDEVGRLQCEHKYHVACIQQWLRMKNWCPICKSTA--LSPFQ 526

BLAST of MS008434 vs. TAIR 10
Match: AT5G24870.1 (RING/U-box superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 8.6e-64
Identity = 206/549 (37.52%), Postives = 290/549 (52.82%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN---HGSRLGCTGRICSSSGAEVGCSS 62
           MD +  +R    I+ RK +G+VL +NMK +D  +     SR+GC+ R+ S+ G  +   +
Sbjct: 1   MDGFKGKRTSRPIMPRKASGLVLHENMKKKDDKSVVPICSRIGCSSRVSSTKGDLIDHKA 60

Query: 63  KASSKRPFRSS-SGKEIVGSSSRSSSIRNFRKSFPDPFGK--LSSKLETDSSEDGSIQDE 122
           KA+    FRS  SGKE VGSSSRS S     K      G+  LSS L+ DSSE  S+ ++
Sbjct: 61  KATVS-SFRSPLSGKETVGSSSRSMSGFGGTKKASKVIGRRQLSSLLDMDSSESSSVNED 120

Query: 123 LEELEFISPPGMF-HTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQD 182
               E   P G    + + V SESSV  + ++ E GSSS  +     R+  QR  + ++D
Sbjct: 121 SPTSERSLPRGKTKESTISVHSESSVSGE-VVTEAGSSSRGTG----RSIHQRPDLVSRD 180

Query: 183 TSTSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDS 242
              S   ++   ++N    +   R+L   S S+++P +S+       R+ N+ +K   D 
Sbjct: 181 ARVSNSEQNARASVN----KNGLRDLRNKSGSDVLPSNSTP-----TRKSNIFRKKTSDG 240

Query: 243 ENSSSSRGKKVSGTSCGQ---NCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACD 302
           E+SSSSRG K  G+  G    +     GI++S+ R+ RN       L S+   S+ S+  
Sbjct: 241 ESSSSSRGNKTEGSVVGGKNISSPQGNGITMSEPRRNRN-------LPSVRDNSVVSSST 300

Query: 303 GASHYYQGISGDRSLQDSLMTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYS 362
             S  Y G +G      +L     P       S ++ + +SP  S+SR   +        
Sbjct: 301 RRSTGYYGRTGRAGAVATLQAPRPPTRADLNPSRSAEASRSPLNSYSRPISS----NGRL 360

Query: 363 QSLLDIGQTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEELT--------- 422
           +SL+  G  + +E G+S +  N ++FR  N++G+AEVLLALERIEQ+EELT         
Sbjct: 361 RSLMMPG--SPSEAGLSRSLMNRDTFRRYNMNGVAEVLLALERIEQDEELTYEQLAVLET 420

Query: 423 -------------------------YEELLDLEERMGTVSTALSEEALTECLNRSIFQSK 482
                                    YEELL LEE+MGTVSTALSEEAL + L  SI++  
Sbjct: 421 NLFLNGMSSFHDQHRDMRLDIDNMSYEELLALEEKMGTVSTALSEEALLKSLKSSIYRPN 480

Query: 483 PQGGALTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKT 507
            +   +  + +D  DVKC ICQEEYV GDEVG L C+HKYHV C QQWLR+KNWCPICKT
Sbjct: 481 DESDDICLNKDD--DVKCSICQEEYVDGDEVGTLPCQHKYHVSCAQQWLRMKNWCPICKT 519

BLAST of MS008434 vs. TAIR 10
Match: AT5G24870.2 (RING/U-box superfamily protein )

HSP 1 Score: 242.3 bits (617), Expect = 8.6e-64
Identity = 206/549 (37.52%), Postives = 290/549 (52.82%), Query Frame = 0

Query: 3   MDEYSSRRVHVGIVSRKGTGVVLRDNMKNRDHSN---HGSRLGCTGRICSSSGAEVGCSS 62
           MD +  +R    I+ RK +G+VL +NMK +D  +     SR+GC+ R+ S+ G  +   +
Sbjct: 1   MDGFKGKRTSRPIMPRKASGLVLHENMKKKDDKSVVPICSRIGCSSRVSSTKGDLIDHKA 60

Query: 63  KASSKRPFRSS-SGKEIVGSSSRSSSIRNFRKSFPDPFGK--LSSKLETDSSEDGSIQDE 122
           KA+    FRS  SGKE VGSSSRS S     K      G+  LSS L+ DSSE  S+ ++
Sbjct: 61  KATVS-SFRSPLSGKETVGSSSRSMSGFGGTKKASKVIGRRQLSSLLDMDSSESSSVNED 120

Query: 123 LEELEFISPPGMF-HTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQD 182
               E   P G    + + V SESSV  + ++ E GSSS  +     R+  QR  + ++D
Sbjct: 121 SPTSERSLPRGKTKESTISVHSESSVSGE-VVTEAGSSSRGTG----RSIHQRPDLVSRD 180

Query: 183 TSTSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDS 242
              S   ++   ++N    +   R+L   S S+++P +S+       R+ N+ +K   D 
Sbjct: 181 ARVSNSEQNARASVN----KNGLRDLRNKSGSDVLPSNSTP-----TRKSNIFRKKTSDG 240

Query: 243 ENSSSSRGKKVSGTSCGQ---NCGYRRGISISDQRQGRNMFHRQNVLSSLGTRSLASACD 302
           E+SSSSRG K  G+  G    +     GI++S+ R+ RN       L S+   S+ S+  
Sbjct: 241 ESSSSSRGNKTEGSVVGGKNISSPQGNGITMSEPRRNRN-------LPSVRDNSVVSSST 300

Query: 303 GASHYYQGISGDRSLQDSLMTSWMPQSNISGSSHTSSSLQSPSESHSRHHMTYPEHEDYS 362
             S  Y G +G      +L     P       S ++ + +SP  S+SR   +        
Sbjct: 301 RRSTGYYGRTGRAGAVATLQAPRPPTRADLNPSRSAEASRSPLNSYSRPISS----NGRL 360

Query: 363 QSLLDIGQTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEELT--------- 422
           +SL+  G  + +E G+S +  N ++FR  N++G+AEVLLALERIEQ+EELT         
Sbjct: 361 RSLMMPG--SPSEAGLSRSLMNRDTFRRYNMNGVAEVLLALERIEQDEELTYEQLAVLET 420

Query: 423 -------------------------YEELLDLEERMGTVSTALSEEALTECLNRSIFQSK 482
                                    YEELL LEE+MGTVSTALSEEAL + L  SI++  
Sbjct: 421 NLFLNGMSSFHDQHRDMRLDIDNMSYEELLALEEKMGTVSTALSEEALLKSLKSSIYRPN 480

Query: 483 PQGGALTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWCPICKT 507
            +   +  + +D  DVKC ICQEEYV GDEVG L C+HKYHV C QQWLR+KNWCPICKT
Sbjct: 481 DESDDICLNKDD--DVKCSICQEEYVDGDEVGTLPCQHKYHVSCAQQWLRMKNWCPICKT 519

BLAST of MS008434 vs. TAIR 10
Match: AT5G10650.1 (RING/U-box superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 2.3e-53
Identity = 192/549 (34.97%), Postives = 269/549 (49.00%), Query Frame = 0

Query: 15  IVSRKGTGVVLRDNMKNRDHSN--HGSRLGCTGRICSSSGAEVGC---SSKASSKRPFRS 74
           +V RK +G+ LR+NM   D  N    SR+GCT ++ S+  + +G    ++K        +
Sbjct: 14  VVPRKASGLTLRENMNKTDGKNVPFCSRVGCTAKVTSTKRSRIGSTDNNTKVGLPPVPST 73

Query: 75  SSGKEIVGSSSRSSSIRNFRKSFPDPFGKL------------SSKLETDSSEDGSIQDEL 134
            + KEIVGSSSR+          P  FG L            SS L+T+SSE   I D+ 
Sbjct: 74  LNRKEIVGSSSRT----------PGGFGYLRKPAKVTARRQPSSSLDTESSETSCIHDDP 133

Query: 135 EELEFISP-PGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDT 194
              E   P        ++V  +S+V  + ++ + GSSS  ++  S   S           
Sbjct: 134 AATEPTLPRQKTKRVTINVHPQSAVSREVVITKAGSSSRGTSRISHPKSELGTRDALTGP 193

Query: 195 STSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSE 254
           S S  S +    + GG SR+  RNL CNSVS+++P +S+S       + +VTKK   D E
Sbjct: 194 SVSTSSGNSEHTVRGGLSRHRLRNLSCNSVSDVLPTNSNS-----ATKISVTKKKNADGE 253

Query: 255 NSSSSRGKKVS---GTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRS--LASAC 314
           +S SS+G K S        Q   +  G+++SD R+ R       V+ S+   S  +++ C
Sbjct: 254 SSLSSKGSKTSVLVPKVRNQISSHGNGVTVSDNRRNR-------VVPSIRDSSTVVSNGC 313

Query: 315 DGASHYYQGISGDRSLQDSLMTS-WMPQSNISGSSHTSSSLQSPSESHS---RHHMTYPE 374
             A ++  G S       S  TS  MP        + S S   PS  +S   R H   P 
Sbjct: 314 RRAGYF--GRSERLGATASSATSRQMPHPTTPTDPNPSLSF-CPSNIYSSTGRVHSNMP- 373

Query: 375 HEDYSQSLLDIGQTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEELT---- 434
                      G  T A+   SS+  N +     N++GIAEVLLALERIE +EELT    
Sbjct: 374 -----------GSPTEADP--SSSLVNRDGLSHYNMNGIAEVLLALERIEHDEELTYEQL 433

Query: 435 ------------------------------YEELLDLEERMGTVSTALSEEALTECLNRS 494
                                         YEELL L ++MGTVSTALSEEAL+  L +S
Sbjct: 434 ASIETNLFSSGMFRFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTALSEEALSRSLKQS 493

Query: 495 IFQSKPQGGALTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWC 503
           I+Q   + G+++   +D  D+KC ICQEEYV GDE+G + C+H YHV C+QQWLR+KNWC
Sbjct: 494 IYQETDETGSISLYKDD--DIKCSICQEEYVDGDELGTIPCQHMYHVSCVQQWLRMKNWC 521

BLAST of MS008434 vs. TAIR 10
Match: AT5G10650.2 (RING/U-box superfamily protein )

HSP 1 Score: 207.6 bits (527), Expect = 2.3e-53
Identity = 192/549 (34.97%), Postives = 269/549 (49.00%), Query Frame = 0

Query: 15  IVSRKGTGVVLRDNMKNRDHSN--HGSRLGCTGRICSSSGAEVGC---SSKASSKRPFRS 74
           +V RK +G+ LR+NM   D  N    SR+GCT ++ S+  + +G    ++K        +
Sbjct: 14  VVPRKASGLTLRENMNKTDGKNVPFCSRVGCTAKVTSTKRSRIGSTDNNTKVGLPPVPST 73

Query: 75  SSGKEIVGSSSRSSSIRNFRKSFPDPFGKL------------SSKLETDSSEDGSIQDEL 134
            + KEIVGSSSR+          P  FG L            SS L+T+SSE   I D+ 
Sbjct: 74  LNRKEIVGSSSRT----------PGGFGYLRKPAKVTARRQPSSSLDTESSETSCIHDDP 133

Query: 135 EELEFISP-PGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQRYGVDNQDT 194
              E   P        ++V  +S+V  + ++ + GSSS  ++  S   S           
Sbjct: 134 AATEPTLPRQKTKRVTINVHPQSAVSREVVITKAGSSSRGTSRISHPKSELGTRDALTGP 193

Query: 195 STSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKNVTKKMIYDSE 254
           S S  S +    + GG SR+  RNL CNSVS+++P +S+S       + +VTKK   D E
Sbjct: 194 SVSTSSGNSEHTVRGGLSRHRLRNLSCNSVSDVLPTNSNS-----ATKISVTKKKNADGE 253

Query: 255 NSSSSRGKKVS---GTSCGQNCGYRRGISISDQRQGRNMFHRQNVLSSLGTRS--LASAC 314
           +S SS+G K S        Q   +  G+++SD R+ R       V+ S+   S  +++ C
Sbjct: 254 SSLSSKGSKTSVLVPKVRNQISSHGNGVTVSDNRRNR-------VVPSIRDSSTVVSNGC 313

Query: 315 DGASHYYQGISGDRSLQDSLMTS-WMPQSNISGSSHTSSSLQSPSESHS---RHHMTYPE 374
             A ++  G S       S  TS  MP        + S S   PS  +S   R H   P 
Sbjct: 314 RRAGYF--GRSERLGATASSATSRQMPHPTTPTDPNPSLSF-CPSNIYSSTGRVHSNMP- 373

Query: 375 HEDYSQSLLDIGQTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEELT---- 434
                      G  T A+   SS+  N +     N++GIAEVLLALERIE +EELT    
Sbjct: 374 -----------GSPTEADP--SSSLVNRDGLSHYNMNGIAEVLLALERIEHDEELTYEQL 433

Query: 435 ------------------------------YEELLDLEERMGTVSTALSEEALTECLNRS 494
                                         YEELL L ++MGTVSTALSEEAL+  L +S
Sbjct: 434 ASIETNLFSSGMFRFYDQHRDMRLDIDNMSYEELLALGDKMGTVSTALSEEALSRSLKQS 493

Query: 495 IFQSKPQGGALTSSVEDLSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQWLRLKNWC 503
           I+Q   + G+++   +D  D+KC ICQEEYV GDE+G + C+H YHV C+QQWLR+KNWC
Sbjct: 494 IYQETDETGSISLYKDD--DIKCSICQEEYVDGDELGTIPCQHMYHVSCVQQWLRMKNWC 521

BLAST of MS008434 vs. TAIR 10
Match: AT4G31450.1 (RING/U-box superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 5.8e-44
Identity = 181/554 (32.67%), Postives = 272/554 (49.10%), Query Frame = 0

Query: 3   MDEYSSRR-VHVGIVSRKGTGVVLRDNMKNRDHSNHGSRLGCTGRICSSSGAE------V 62
           MD +SS+R +     S+K  GVV RD    R +SN      C   ICS           V
Sbjct: 1   MDRWSSKRAMEARPDSKKKGGVVFRD----RFNSN-----SCKVPICSDEKKSMNFTRFV 60

Query: 63  GCSSK--ASSKRPFRSS-SGKEIVGSSSR-----SSSIRNFRKSFPDPFGKLSSKLETDS 122
           G S K   S    +RSS +GKE++G+SS+     SSS+++  K    PF +++  +++  
Sbjct: 61  GSSDKKEKSVLSTYRSSPNGKEVIGTSSKICISSSSSVKSGEK---QPFSQIA--IDSSE 120

Query: 123 SEDGSIQDELEELEFISPPGMFHTGLHVKSESSVPADAILMERGSSSTDSNTKSRRNSIQ 182
           S  GS +DE+E      P G     ++ K    +    I  E   S   S+++ +R   Q
Sbjct: 121 SSRGS-EDEVESEILQVPLGRDKRRMNNK---LIYGKVITPEAECSKLPSSSRIKRGFRQ 180

Query: 183 RYGVDNQDTSTSMRSRSLCQALNGGTSRYVSRNLECNSVSEIIPPDSSSKEQNLVRRKN- 242
           R+G+  Q+               G + +  S N  C+ +   + P     ++ L R+ + 
Sbjct: 181 RFGLSKQEFHP------------GPSGQSTSANRGCSPLLSGVIPSGFGLDKRLSRKADT 240

Query: 243 VTKKMIY-DSENSSSSRGKKVS---GTSCGQNCGYRRGISISDQRQGRN-MFHRQNVLSS 302
           ++K  +Y +S +SSS+RGK V+        +     RG S+SD R+ R+ +    N ++S
Sbjct: 241 ISKTKVYGESSSSSSARGKNVTEPPPVEVRRRSFNPRG-SVSDSRRARHCILDDDNDVAS 300

Query: 303 LGTRSLASACDGASHYYQGISGDRSLQDSLMTSWMPQSNISGSSHTSSSLQSPSESHSRH 362
           +G++ LA+  +            R    S+  + M Q+  S + ++  SL+  S      
Sbjct: 301 VGSQRLANRNNSRIR-----GSGRDGLSSVTAAEMSQTETSNNLNSPVSLELFS------ 360

Query: 363 HMTYPEHEDYSQSLLDIGQTTAAEGGISSTSTNLNSFRCRNVDGIAEVLLALERIEQEEE 422
              +PE                   G+S +  + +SFR  N+DGI+E+L  L+RIEQ+ E
Sbjct: 361 --GFPEF------------------GLSGSLLSHDSFRSYNLDGISEILPELDRIEQDIE 420

Query: 423 L---------------------------------TYEELLDLEERMGTVSTALSEEALTE 482
           L                                 +YEELL LEER+GTVSTAL+EEA+++
Sbjct: 421 LNYEDLLIMETGLLLGGLSFHDQHRDMRLDIDNMSYEELLALEERIGTVSTALTEEAISK 480

Query: 483 CLNRSIFQSKPQG-GALTSSVED-LSDVKCCICQEEYVSGDEVGRLHCEHKYHVKCIQQW 501
           CL  SI+Q KP   G++T S  D   D KC ICQEEY  GDEVGRLHCEH YHVKC+Q+W
Sbjct: 481 CLKTSIYQMKPLSYGSITKSPSDNKEDAKCSICQEEYTIGDEVGRLHCEHTYHVKCVQEW 492