MS008407 (gene) Bitter gourd (TR) v1

Overview
NameMS008407
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionrhomboid-like protein 11, chloroplastic
Locationscaffold4: 1163721 .. 1166691 (+)
RNA-Seq ExpressionMS008407
SyntenyMS008407
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCAATTGCAGTCGCAGCTTCATGGATTCCGATTATCGAAACCTTGTGGATTAAGCAAGCGAATTCCCATGGCTTCAATTTCTCGTCTCTCCAGTTTCAGCTCAAGCTCGCTCAGATTCCCCTCTCTATTATTTCATCCGCTCTCATTGTCTCACTCCGTTGCTCGGCTTCCCTATGTTCTTCGCCTCCGCGCTCGTTCCGTAGCCGCGAAACCCGTCTCCATGTGCAAAATGAACGATTCAGGTTGTTTTTCTGCGGTATTTGCCGATTATTTGCACCTTGCAATGCAATATATTTGTTCAGTGATGATGATGTGTGCGATTGAATGAAAATAAAGAAGAATTTTGTTTTTAAATGTTTGTTAGATTTGATATCACAGTTGGAGCTGGGGAAACCAGTGCAGAGTCGGAAGCCGGAGAAGCGTGTGAACGGTATATTCTGGATTATTCTTCTCAACATTGGAATATATGTGGCTGATCACATTTTTCAGGTAGGGTTTTGTGTTCCCTTATACTGTTTAAGTCTCTCGTTGTTGTCTTCGGGTAGAAAGTTGGCTGGTTGCTGAGAAGTTTGATGATATTTTTTTAGGTCAAACATTTCTGTTCTATTCAGCATTCACCCGTGTGAGTGAATTAGCTATCTGCCAAAGCGGTTTGTTTATAGGGTTTTAAGGGGATAATCCACTGTTAAGCGGATTATGATCAGTGAAACTTTTCAGAATATGAAAAGATTTTATCCAAAAAGCATTAAACCAGAAACCAAAAGGAGGATTGCAAAAAGGAGGTTCAATTGTTATAAACAAAAAAAGGGTGTAATTACAAAGGGTTTTCTAGAGAGTATACCATCTTAAAGTAAAATATTGTAAGAAACTAAATCAAAGAAGTCTTCAAAATCTCAATGAGAGCCATTGAGAGTTCTCCAATTGCACACCGTCCACAGCTCCCAAAGGCCAATCCCGGCAGTTTTCCCACCATACCTTAGCTTTTCTCTGAAACGTCGCCACACAGAAAATCCTGAATAAATACTTAGATGGTCTATGGCTTACTCCAACTAAAACTAAACTTCTGAAAGAGTCTGCCATGATTTTGAAGCATAAGTACAGTGAACAAAAAAGATGGCTTTGGCTACCTCACTATTCTTGCACAAAATGCACCAGTTGGAAGAAGAAAAGAGTTCGAAATTTTTTTCTGCAGCACACCAAGGGAGTCGAGTTTCTCCACCAACGAAGTACATGAAAATACTCACCTTCCTTGGGATTTTTTCCTCCAAATACAGGTCGTTAACTGGTTGAGAAGAATTTTTTGAGCCCTCACAAGGTGAACAAAGTGGGATGTTCTAGAGATCTCTCTTGAAACTTCCGAAGACCAAATCCTCTTGTGGTCCCTAGAATCAAGATCTGTAGCAGTCGTCTTAGCCAACAAAATAGCCAAAGATTCAATTTTCCTGTAGATTCATTCTAAGAGTCAGATTCCAATCTCTTCACCTCATCCCACATGTCTTGCACACCAGAGCTTTGTTCCTAAATACCAAGTTGTCTATACCAAACGACCACTATCCGATGGAAGAGTAACCAGTGACCATCTAACTAAATGACAAAAAAAGGAACCAATTCACTTTAAAATTAAAAAGACCCCAAGAAACAATTTCCATCATTGATTGTGTTTCATCATCAATTTTAACTTGTAAGATTGAAAAGCATGAGGACAATGACTGAGTTATCTTTTCCATAGGTTCGTGGTATAGGAGCCCTGTACCTGTACCACAATCGTCCTGCTTGGTATCAGTTTTTTACAGCAACATTTTGTCATGCTAATTGGTGAGTTTTTTGTGCTCTTTCAAAGATCATGCAAACTTCAGATTTCTCGTATACTTAAAAGGATCACTGGAGTTTACATAAATATATATATAGAAGCTTGCTTATCTTCTCACGAGAATGCCGTTTGCTGGTTCTACGAGCATGGTTTGATGCAGTACTTGCTATTTCTATGAATGTTTGCAGGAACCATCTCTCTAGCAATCTTTTTTTCCTGTATATATTTGGTGAGAGCCGCTTTCTTCCCCCTGCCCAAAGAAAATAATATTATATTATTTTTCTCCCAAATATCTAAGGTTTCTACTTTTCTTTCTAGAAGGTGGAAACCATAAAGTAACCAGAATGTTTTGACATTTAATAGCTCCATTATTTGTGATGAACAGGAAAACTTGTTGAGGAAGAGGAAGGAAACTTTGCTTTGTGGCTCTCCTATATTCTTACCGGTGTAGGTGCAAACATTGTGTCATGGCTAATTTTACCAAGAAATGCTGTTTCGGTTGGAGCTTCTGGTGCTGTTTTTGGGTTGTTTACCATAAGTGTCCTCGTAAAGGTATCCATCTAAAACAATGATTCAACATGTCATCAGGATTTTAATGTATTTTTGCTAAGTAACGTCCTTCATGGCAGATGTCATGGGATTGGAGGAAGATCCTCGAAGTCCTTATACTTGGTCAATTCGTTATAGAGAAGGTAATAAGTTCTCTTTTTATCCCCATGTGCATGATTTTAAATGCTTCTAACCGATAAACATCCTTCCCGAATCGGGTCTTTGGCATGTTATATCATATTCAAGATTCTCTTTAGTCTTTTGCTCTATCAACCATAAGAATACCACAAGTAACTGAATTGATATTATCCTAGAAATTGAAAGAAACAATTTTTTCAATGTCCGAATTCGTTCGAGTGAAAGGAATGGTGAAACCGTGAAGGGCATGCACTTATCCATAAATCTTTTTTGGTCATAATAGGTCATGGAAGCTGCCCAAGCTTCGACTGCCCTATCAGGAAGCTTTCGTGGAGGATATTCCTTGCAGAATGTCAACCATGTTGCCCATCTCTCCGGGGCGCTTGTCGGGGTCGTTCTAGTATGGCTGCTGAGTAGAATTCCGTCTCCTCCTGACCAAGACGTATCGCCTTTGAATAGGAAAGGAAGA

mRNA sequence

ATGAAGCAATTGCAGTCGCAGCTTCATGGATTCCGATTATCGAAACCTTGTGGATTAAGCAAGCGAATTCCCATGGCTTCAATTTCTCGTCTCTCCAGTTTCAGCTCAAGCTCGCTCAGATTCCCCTCTCTATTATTTCATCCGCTCTCATTGTCTCACTCCGTTGCTCGGCTTCCCTATGTTCTTCGCCTCCGCGCTCGTTCCGTAGCCGCGAAACCCGTCTCCATGTGCAAAATGAACGATTCAGATTTGATATCACAGTTGGAGCTGGGGAAACCAGTGCAGAGTCGGAAGCCGGAGAAGCGTGTGAACGGTATATTCTGGATTATTCTTCTCAACATTGGAATATATGTGGCTGATCACATTTTTCAGGTTCGTGGTATAGGAGCCCTGTACCTGTACCACAATCGTCCTGCTTGGTATCAGTTTTTTACAGCAACATTTTGTCATGCTAATTGGAACCATCTCTCTAGCAATCTTTTTTTCCTGTATATATTTGGAAAACTTGTTGAGGAAGAGGAAGGAAACTTTGCTTTGTGGCTCTCCTATATTCTTACCGGTGTAGGTGCAAACATTGTGTCATGGCTAATTTTACCAAGAAATGCTGTTTCGGTTGGAGCTTCTGGTGCTGTTTTTGGGTTGTTTACCATAAGTGTCCTCGTAAAGATGTCATGGGATTGGAGGAAGATCCTCGAAGTCCTTATACTTGGTCAATTCGTTATAGAGAAGGTCATGGAAGCTGCCCAAGCTTCGACTGCCCTATCAGGAAGCTTTCGTGGAGGATATTCCTTGCAGAATGTCAACCATGTTGCCCATCTCTCCGGGGCGCTTGTCGGGGTCGTTCTAGTATGGCTGCTGAGTAGAATTCCGTCTCCTCCTGACCAAGACGTATCGCCTTTGAATAGGAAAGGAAGA

Coding sequence (CDS)

ATGAAGCAATTGCAGTCGCAGCTTCATGGATTCCGATTATCGAAACCTTGTGGATTAAGCAAGCGAATTCCCATGGCTTCAATTTCTCGTCTCTCCAGTTTCAGCTCAAGCTCGCTCAGATTCCCCTCTCTATTATTTCATCCGCTCTCATTGTCTCACTCCGTTGCTCGGCTTCCCTATGTTCTTCGCCTCCGCGCTCGTTCCGTAGCCGCGAAACCCGTCTCCATGTGCAAAATGAACGATTCAGATTTGATATCACAGTTGGAGCTGGGGAAACCAGTGCAGAGTCGGAAGCCGGAGAAGCGTGTGAACGGTATATTCTGGATTATTCTTCTCAACATTGGAATATATGTGGCTGATCACATTTTTCAGGTTCGTGGTATAGGAGCCCTGTACCTGTACCACAATCGTCCTGCTTGGTATCAGTTTTTTACAGCAACATTTTGTCATGCTAATTGGAACCATCTCTCTAGCAATCTTTTTTTCCTGTATATATTTGGAAAACTTGTTGAGGAAGAGGAAGGAAACTTTGCTTTGTGGCTCTCCTATATTCTTACCGGTGTAGGTGCAAACATTGTGTCATGGCTAATTTTACCAAGAAATGCTGTTTCGGTTGGAGCTTCTGGTGCTGTTTTTGGGTTGTTTACCATAAGTGTCCTCGTAAAGATGTCATGGGATTGGAGGAAGATCCTCGAAGTCCTTATACTTGGTCAATTCGTTATAGAGAAGGTCATGGAAGCTGCCCAAGCTTCGACTGCCCTATCAGGAAGCTTTCGTGGAGGATATTCCTTGCAGAATGTCAACCATGTTGCCCATCTCTCCGGGGCGCTTGTCGGGGTCGTTCTAGTATGGCTGCTGAGTAGAATTCCGTCTCCTCCTGACCAAGACGTATCGCCTTTGAATAGGAAAGGAAGA

Protein sequence

MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPYVLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVADHIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPPDQDVSPLNRKGR
Homology
BLAST of MS008407 vs. NCBI nr
Match: XP_022142618.1 (rhomboid-like protein 11, chloroplastic [Momordica charantia])

HSP 1 Score: 599.7 bits (1545), Expect = 1.4e-167
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60
           MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY
Sbjct: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60

Query: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120
           VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD
Sbjct: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120

Query: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180
           HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW
Sbjct: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180

Query: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240
           LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV
Sbjct: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240

Query: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPPDQDVSPL 300
           IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPPDQDVSPL
Sbjct: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPPDQDVSPL 300

Query: 301 NRKGR 306
           NRKGR
Sbjct: 301 NRKGR 305

BLAST of MS008407 vs. NCBI nr
Match: XP_023524317.1 (rhomboid-like protein 11, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 512.3 bits (1318), Expect = 2.9e-141
Identity = 265/306 (86.60%), Postives = 280/306 (91.50%), Query Frame = 0

Query: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60
           M+    QLHG R+ KP GL +++   SI+ LSSFSSSSLRF S LFHPLS S SVA+   
Sbjct: 1   MQSRLQQLHGSRILKPRGLREQMATTSIAHLSSFSSSSLRFSSPLFHPLSSSLSVAQFSN 60

Query: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120
           VLRLRARS AAKP++MCKMND+DLISQLELGKP+Q+RKPEKRVNGIFWIILLNIG+YVAD
Sbjct: 61  VLRLRARSTAAKPIAMCKMNDADLISQLELGKPLQNRKPEKRVNGIFWIILLNIGVYVAD 120

Query: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180
           HIFQVR IGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW
Sbjct: 121 HIFQVRSIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180

Query: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240
           LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV
Sbjct: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240

Query: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPS-PPDQDVSP 300
           IEKVMEAAQASTALSGSFRGG +LQNVNHVAHLSGALVGVVLVWLLSRIPS PPDQD S 
Sbjct: 241 IEKVMEAAQASTALSGSFRGGSALQNVNHVAHLSGALVGVVLVWLLSRIPSQPPDQDAST 300

Query: 301 LNRKGR 306
           L+RK R
Sbjct: 301 LHRKVR 306

BLAST of MS008407 vs. NCBI nr
Match: XP_022981184.1 (rhomboid-like protein 11, chloroplastic [Cucurbita maxima])

HSP 1 Score: 508.1 bits (1307), Expect = 5.4e-140
Identity = 263/306 (85.95%), Postives = 278/306 (90.85%), Query Frame = 0

Query: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60
           M+    QLHG R+ KP GL +++   SI+ LS FSSSSLRF S LFHPLS S SVA+   
Sbjct: 1   MQSRLQQLHGSRILKPRGLREQMATTSIAHLSRFSSSSLRFSSPLFHPLSSSISVAQFSN 60

Query: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120
           VLRLRARS AAKP++MCKMND+DLISQLELGKP+Q+RKPEKRVNGIFWIILLNIG+YVAD
Sbjct: 61  VLRLRARSTAAKPIAMCKMNDADLISQLELGKPLQNRKPEKRVNGIFWIILLNIGVYVAD 120

Query: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180
           HIFQVR IG LYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW
Sbjct: 121 HIFQVRSIGNLYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180

Query: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240
           LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV
Sbjct: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240

Query: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPS-PPDQDVSP 300
           IEKVMEAAQASTALSGSFRGG +LQNVNHVAHLSGALVGVVLVWLLSRIPS PPDQD S 
Sbjct: 241 IEKVMEAAQASTALSGSFRGGSALQNVNHVAHLSGALVGVVLVWLLSRIPSQPPDQDAST 300

Query: 301 LNRKGR 306
           L+RK R
Sbjct: 301 LHRKVR 306

BLAST of MS008407 vs. NCBI nr
Match: XP_038896989.1 (rhomboid-like protein 11, chloroplastic [Benincasa hispida])

HSP 1 Score: 505.4 bits (1300), Expect = 3.5e-139
Identity = 260/304 (85.53%), Postives = 276/304 (90.79%), Query Frame = 0

Query: 3   QLQ-SQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPYV 62
           QLQ  QLHG R+SKP  L K++PM SIS LSSF+ +S   PS LFHP S SHS+ R   V
Sbjct: 2   QLQLQQLHGCRISKPHSLCKQMPMTSISLLSSFTLTSFSLPSPLFHPFSSSHSLPRFSDV 61

Query: 63  LRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVADH 122
           LRLR RSV AKP++MCKMND+DLISQLELGKP+Q+RKPE+RVNGIFWIILLNIGIYVADH
Sbjct: 62  LRLRTRSVCAKPIAMCKMNDADLISQLELGKPLQNRKPERRVNGIFWIILLNIGIYVADH 121

Query: 123 IFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWL 182
           IFQVR IG LYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKL+EEEEGNFALWL
Sbjct: 122 IFQVRSIGVLYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLLEEEEGNFALWL 181

Query: 183 SYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVI 242
           SYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVI
Sbjct: 182 SYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVI 241

Query: 243 EKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPP-DQDVSPL 302
           EKVMEAAQASTALSGSFRGG+SLQNVNHVAHLSGALVGV+LVWLLSRIPSP  DQD S L
Sbjct: 242 EKVMEAAQASTALSGSFRGGHSLQNVNHVAHLSGALVGVLLVWLLSRIPSPSHDQDASTL 301

Query: 303 NRKG 305
           + KG
Sbjct: 302 HTKG 305

BLAST of MS008407 vs. NCBI nr
Match: XP_022940340.1 (rhomboid-like protein 11, chloroplastic [Cucurbita moschata])

HSP 1 Score: 503.1 bits (1294), Expect = 1.7e-138
Identity = 261/307 (85.02%), Postives = 276/307 (89.90%), Query Frame = 0

Query: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60
           M+    QLHG R+ KP GL +++   SI+ LS FSSSSLRF S LFHPLS S SVA+   
Sbjct: 1   MQSRLQQLHGSRILKPRGLREQMATTSIAHLSRFSSSSLRFSSPLFHPLSASLSVAQFSN 60

Query: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120
           VLRLRARS AAKP++MCKMND+DLISQLELGKP+Q+RKPEKRVNGIFWIILLNIG+YVAD
Sbjct: 61  VLRLRARSTAAKPIAMCKMNDADLISQLELGKPLQNRKPEKRVNGIFWIILLNIGVYVAD 120

Query: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180
           HIFQVR IG LYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW
Sbjct: 121 HIFQVRSIGNLYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180

Query: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240
           LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV
Sbjct: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240

Query: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPS--PPDQDVS 300
           IEKVMEAAQASTALSGSFRGG +LQNVNHVAHLSGALVGV LVWLLSRIPS  P DQD S
Sbjct: 241 IEKVMEAAQASTALSGSFRGGSALQNVNHVAHLSGALVGVFLVWLLSRIPSQPPDDQDAS 300

Query: 301 PLNRKGR 306
            L+RK R
Sbjct: 301 TLHRKVR 307

BLAST of MS008407 vs. ExPASy Swiss-Prot
Match: Q84MB5 (Rhomboid-like protein 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL11 PE=1 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 4.7e-102
Identity = 199/292 (68.15%), Postives = 221/292 (75.68%), Query Frame = 0

Query: 8   LHGFRLSKP-CGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPYVLRLRA 67
           LH  RLS P   L  R P     R +S  ++S + P L F PL++S S            
Sbjct: 5   LHLHRLSLPQSSLRFRFPPLHRRRAASSPTNSTQ-PPLQFRPLTVSRSQI---------- 64

Query: 68  RSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVADHIFQVR 127
                     C+ + SD+  Q EL K   +RKP+KR NGIFWIIL+N+GIY+ADH FQVR
Sbjct: 65  ---------TCRFSQSDITPQFELDKAKDNRKPQKRANGIFWIILINLGIYLADHFFQVR 124

Query: 128 GIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILT 187
           GI +LYLYHN PAWYQF TATFCHANWNHLSSNLFFLYIFGKLVEEEEGNF LWLSY+ T
Sbjct: 125 GIKSLYLYHNFPAWYQFVTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFGLWLSYLFT 184

Query: 188 GVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEKVME 247
           GVGAN+VSWL+LPRNAVSVGASGAVFGLF ISVLVKMSWDWRKILEVLILGQFVIE+VME
Sbjct: 185 GVGANLVSWLVLPRNAVSVGASGAVFGLFAISVLVKMSWDWRKILEVLILGQFVIERVME 244

Query: 248 AAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPP-DQDV 298
           AAQAS  LSG+  GGYSLQ VNH+AHLSGALVGVVLVWLLS+ PS   DQDV
Sbjct: 245 AAQASAGLSGTIYGGYSLQTVNHIAHLSGALVGVVLVWLLSKFPSASMDQDV 276

BLAST of MS008407 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.7e-06
Identity = 48/153 (31.37%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 141 YQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILTGVGANIVSWLILPR 200
           ++  TA + HA   HL  N+F + IFG  +E++ G   + L Y+++G G +I+S L L +
Sbjct: 98  WRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL-Q 157

Query: 201 NAVSVGASGAVFGLFTISVLVKMSWDW---RKILEVLILGQFVIEKVMEAAQASTALSGS 260
            ++SVGASGA+ GL   ++L ++  +W   +  L  L+   F+I     A   +  L   
Sbjct: 158 KSISVGASGALLGLMG-AMLSELLTNWTIYKSKLCALLSFLFII-----AINLAIGL--- 217

Query: 261 FRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIP 291
                 L  V++ AH+ G L G  L ++L   P
Sbjct: 218 ------LPWVDNFAHIGGLLTGFCLGFILLMQP 234

BLAST of MS008407 vs. ExPASy Swiss-Prot
Match: P96617 (Putative rhomboid protease YdcA OS=Bacillus subtilis (strain 168) OX=224308 GN=ydcA PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.8e-06
Identity = 40/146 (27.40%), Postives = 69/146 (47.26%), Query Frame = 0

Query: 140 WYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILTGVGANIVSWLILP 199
           W++  T    HA + HL  N   +++F   +E   G     L Y  +G+  NI +++  P
Sbjct: 58  WWRLITPILLHAGFTHLLFNSMSIFLFAPALERMLGKARFLLVYAGSGIIGNIGTYVTEP 117

Query: 200 RNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEKVMEAAQASTALSGSFR 259
            + V VGASGA+FGLF + + +           VL   + + ++   +    T L+ +  
Sbjct: 118 LDYVHVGASGAIFGLFGVYLFM-----------VLFRNELIGQE--HSKMIITLLAFAVL 177

Query: 260 GGYSLQNVNHVAHLSGALVGVVLVWL 286
             +   N+N +AHL G   G +L +L
Sbjct: 178 MSFINSNINMMAHLFGLCGGFLLSFL 190

BLAST of MS008407 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.4e-05
Identity = 32/93 (34.41%), Postives = 53/93 (56.99%), Query Frame = 0

Query: 135 HNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILTGVGANIVS 194
           H    W + FT  + HA   H+ +N+  L   G  +E+E G   + L Y+++G G +++S
Sbjct: 125 HKHEVW-RLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFVRIGLLYMISGFGGSLLS 184

Query: 195 WLILPRNAVSVGASGAVFGLFTISVLVKMSWDW 228
            L   R  +SVGASGA+FGL   ++L ++  +W
Sbjct: 185 SL-FNRAGISVGASGALFGLLG-AMLSELLTNW 214

BLAST of MS008407 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 4.1e-05
Identity = 46/155 (29.68%), Postives = 77/155 (49.68%), Query Frame = 0

Query: 135 HNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILTGVGANIVS 194
           H    W +  +  + HA   HL +N+  L   G  +E++ G   + L Y+++G+G +I+S
Sbjct: 106 HEHQGW-RLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGFIRVGLIYLISGLGGSILS 165

Query: 195 WLILPRNAVSVGASGAVFGLFTISVLVKMSWDW---RKILEVLILGQFVIEKVMEAAQAS 254
            L L + ++SVGASGA+FGL   ++L ++  +W         LI   F+I         +
Sbjct: 166 SLFL-QESISVGASGALFGLLG-AMLSELLTNWTIYANKAAALITLLFII-------AIN 225

Query: 255 TALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLL 287
            AL         L  V++ AH+ G L G  L ++L
Sbjct: 226 LALG-------MLPRVDNFAHIGGFLTGFCLGFVL 243

BLAST of MS008407 vs. ExPASy TrEMBL
Match: A0A6J1CNP9 (rhomboid-like protein 11, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111012689 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 6.6e-168
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60
           MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY
Sbjct: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60

Query: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120
           VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD
Sbjct: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120

Query: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180
           HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW
Sbjct: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180

Query: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240
           LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV
Sbjct: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240

Query: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPPDQDVSPL 300
           IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPPDQDVSPL
Sbjct: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPPDQDVSPL 300

Query: 301 NRKGR 306
           NRKGR
Sbjct: 301 NRKGR 305

BLAST of MS008407 vs. ExPASy TrEMBL
Match: A0A6J1J179 (rhomboid-like protein 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111480404 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.6e-140
Identity = 263/306 (85.95%), Postives = 278/306 (90.85%), Query Frame = 0

Query: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60
           M+    QLHG R+ KP GL +++   SI+ LS FSSSSLRF S LFHPLS S SVA+   
Sbjct: 1   MQSRLQQLHGSRILKPRGLREQMATTSIAHLSRFSSSSLRFSSPLFHPLSSSISVAQFSN 60

Query: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120
           VLRLRARS AAKP++MCKMND+DLISQLELGKP+Q+RKPEKRVNGIFWIILLNIG+YVAD
Sbjct: 61  VLRLRARSTAAKPIAMCKMNDADLISQLELGKPLQNRKPEKRVNGIFWIILLNIGVYVAD 120

Query: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180
           HIFQVR IG LYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW
Sbjct: 121 HIFQVRSIGNLYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180

Query: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240
           LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV
Sbjct: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240

Query: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPS-PPDQDVSP 300
           IEKVMEAAQASTALSGSFRGG +LQNVNHVAHLSGALVGVVLVWLLSRIPS PPDQD S 
Sbjct: 241 IEKVMEAAQASTALSGSFRGGSALQNVNHVAHLSGALVGVVLVWLLSRIPSQPPDQDAST 300

Query: 301 LNRKGR 306
           L+RK R
Sbjct: 301 LHRKVR 306

BLAST of MS008407 vs. ExPASy TrEMBL
Match: A0A6J1FQ99 (rhomboid-like protein 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445986 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.4e-139
Identity = 261/307 (85.02%), Postives = 276/307 (89.90%), Query Frame = 0

Query: 1   MKQLQSQLHGFRLSKPCGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPY 60
           M+    QLHG R+ KP GL +++   SI+ LS FSSSSLRF S LFHPLS S SVA+   
Sbjct: 1   MQSRLQQLHGSRILKPRGLREQMATTSIAHLSRFSSSSLRFSSPLFHPLSASLSVAQFSN 60

Query: 61  VLRLRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVAD 120
           VLRLRARS AAKP++MCKMND+DLISQLELGKP+Q+RKPEKRVNGIFWIILLNIG+YVAD
Sbjct: 61  VLRLRARSTAAKPIAMCKMNDADLISQLELGKPLQNRKPEKRVNGIFWIILLNIGVYVAD 120

Query: 121 HIFQVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180
           HIFQVR IG LYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW
Sbjct: 121 HIFQVRSIGNLYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALW 180

Query: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240
           LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV
Sbjct: 181 LSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFV 240

Query: 241 IEKVMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPS--PPDQDVS 300
           IEKVMEAAQASTALSGSFRGG +LQNVNHVAHLSGALVGV LVWLLSRIPS  P DQD S
Sbjct: 241 IEKVMEAAQASTALSGSFRGGSALQNVNHVAHLSGALVGVFLVWLLSRIPSQPPDDQDAS 300

Query: 301 PLNRKGR 306
            L+RK R
Sbjct: 301 TLHRKVR 307

BLAST of MS008407 vs. ExPASy TrEMBL
Match: A0A5D3E659 (Rhomboid-like protein 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006290 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.2e-130
Identity = 248/303 (81.85%), Postives = 267/303 (88.12%), Query Frame = 0

Query: 5   QSQLHGFRLSKPCGLS-KRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPYVLR 64
           Q QLHG R+SKP  LS  ++P+ SIS  SSF+ +SLRFPS  F      HS+ +  +VL 
Sbjct: 7   QLQLHGGRISKPPTLSNNQMPLTSISLQSSFTLTSLRFPSPFF------HSLPQFTHVLP 66

Query: 65  LRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVADHIF 124
           L +RS   KP++ CKMND+DLISQLELGKP+Q+RK EKRVNGIFWIILLNIGIYVADHIF
Sbjct: 67  LHSRSFCMKPIATCKMNDADLISQLELGKPLQNRKTEKRVNGIFWIILLNIGIYVADHIF 126

Query: 125 QVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSY 184
           QVR IGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSY
Sbjct: 127 QVRNIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSY 186

Query: 185 ILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEK 244
           ILTGVGAN+VSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEK
Sbjct: 187 ILTGVGANVVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEK 246

Query: 245 VMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPP-DQDVSPLNR 304
           VMEAAQASTALSGSFRGG+  QNVNHVAHLSGALVGV+LVWLLSRIPS   DQD S L+R
Sbjct: 247 VMEAAQASTALSGSFRGGHPSQNVNHVAHLSGALVGVLLVWLLSRIPSQSLDQDASALHR 303

Query: 305 KGR 306
           KGR
Sbjct: 307 KGR 303

BLAST of MS008407 vs. ExPASy TrEMBL
Match: A0A1S3CQS6 (rhomboid-like protein 11, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503716 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.2e-130
Identity = 248/303 (81.85%), Postives = 267/303 (88.12%), Query Frame = 0

Query: 5   QSQLHGFRLSKPCGLS-KRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPYVLR 64
           Q QLHG R+SKP  LS  ++P+ SIS  SSF+ +SLRFPS  F      HS+ +  +VL 
Sbjct: 7   QLQLHGGRISKPPTLSNNQMPLTSISLQSSFTLTSLRFPSPFF------HSLPQFTHVLP 66

Query: 65  LRARSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVADHIF 124
           L +RS   KP++ CKMND+DLISQLELGKP+Q+RK EKRVNGIFWIILLNIGIYVADHIF
Sbjct: 67  LHSRSFCMKPIATCKMNDADLISQLELGKPLQNRKTEKRVNGIFWIILLNIGIYVADHIF 126

Query: 125 QVRGIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSY 184
           QVR IGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSY
Sbjct: 127 QVRNIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSY 186

Query: 185 ILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEK 244
           ILTGVGAN+VSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEK
Sbjct: 187 ILTGVGANVVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEK 246

Query: 245 VMEAAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPP-DQDVSPLNR 304
           VMEAAQASTALSGSFRGG+  QNVNHVAHLSGALVGV+LVWLLSRIPS   DQD S L+R
Sbjct: 247 VMEAAQASTALSGSFRGGHPSQNVNHVAHLSGALVGVLLVWLLSRIPSQSLDQDASALHR 303

Query: 305 KGR 306
           KGR
Sbjct: 307 KGR 303

BLAST of MS008407 vs. TAIR 10
Match: AT5G25752.1 (rhomboid-like protein 11 )

HSP 1 Score: 372.5 bits (955), Expect = 3.3e-103
Identity = 199/292 (68.15%), Postives = 221/292 (75.68%), Query Frame = 0

Query: 8   LHGFRLSKP-CGLSKRIPMASISRLSSFSSSSLRFPSLLFHPLSLSHSVARLPYVLRLRA 67
           LH  RLS P   L  R P     R +S  ++S + P L F PL++S S            
Sbjct: 5   LHLHRLSLPQSSLRFRFPPLHRRRAASSPTNSTQ-PPLQFRPLTVSRSQI---------- 64

Query: 68  RSVAAKPVSMCKMNDSDLISQLELGKPVQSRKPEKRVNGIFWIILLNIGIYVADHIFQVR 127
                     C+ + SD+  Q EL K   +RKP+KR NGIFWIIL+N+GIY+ADH FQVR
Sbjct: 65  ---------TCRFSQSDITPQFELDKAKDNRKPQKRANGIFWIILINLGIYLADHFFQVR 124

Query: 128 GIGALYLYHNRPAWYQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILT 187
           GI +LYLYHN PAWYQF TATFCHANWNHLSSNLFFLYIFGKLVEEEEGNF LWLSY+ T
Sbjct: 125 GIKSLYLYHNFPAWYQFVTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFGLWLSYLFT 184

Query: 188 GVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVKMSWDWRKILEVLILGQFVIEKVME 247
           GVGAN+VSWL+LPRNAVSVGASGAVFGLF ISVLVKMSWDWRKILEVLILGQFVIE+VME
Sbjct: 185 GVGANLVSWLVLPRNAVSVGASGAVFGLFAISVLVKMSWDWRKILEVLILGQFVIERVME 244

Query: 248 AAQASTALSGSFRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIPSPP-DQDV 298
           AAQAS  LSG+  GGYSLQ VNH+AHLSGALVGVVLVWLLS+ PS   DQDV
Sbjct: 245 AAQASAGLSGTIYGGYSLQTVNHIAHLSGALVGVVLVWLLSKFPSASMDQDV 276

BLAST of MS008407 vs. TAIR 10
Match: AT5G25640.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 116.3 bits (290), Expect = 4.3e-26
Identity = 57/85 (67.06%), Postives = 70/85 (82.35%), Query Frame = 0

Query: 163 LYIFGKLVEEEEGNFALWLSYILTGVGANIVSWLILPRNAVSVGASGAVFGLFTISVLVK 222
           L++ GK+VEEE+GNF LWLSY+ TGVGAN+VSW  LP N+   GASGAVFGLF ISVLVK
Sbjct: 5   LWLTGKIVEEEKGNFGLWLSYLFTGVGANMVSWFTLPPNSFFSGASGAVFGLFVISVLVK 64

Query: 223 MSWDWRKILEVLILGQFVIEKVMEA 248
           MS  W K LEVLILGQFV+++ +++
Sbjct: 65  MSRGWSKFLEVLILGQFVMKRFIDS 89

BLAST of MS008407 vs. TAIR 10
Match: AT1G12750.1 (RHOMBOID-like protein 6 )

HSP 1 Score: 53.9 bits (128), Expect = 2.6e-07
Identity = 48/153 (31.37%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 141 YQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILTGVGANIVSWLILPR 200
           ++  TA + HA   HL  N+F + IFG  +E++ G   + L Y+++G G +I+S L L +
Sbjct: 98  WRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL-Q 157

Query: 201 NAVSVGASGAVFGLFTISVLVKMSWDW---RKILEVLILGQFVIEKVMEAAQASTALSGS 260
            ++SVGASGA+ GL   ++L ++  +W   +  L  L+   F+I     A   +  L   
Sbjct: 158 KSISVGASGALLGLMG-AMLSELLTNWTIYKSKLCALLSFLFII-----AINLAIGL--- 217

Query: 261 FRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIP 291
                 L  V++ AH+ G L G  L ++L   P
Sbjct: 218 ------LPWVDNFAHIGGLLTGFCLGFILLMQP 234

BLAST of MS008407 vs. TAIR 10
Match: AT1G12750.2 (RHOMBOID-like protein 6 )

HSP 1 Score: 53.9 bits (128), Expect = 2.6e-07
Identity = 48/153 (31.37%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 141 YQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILTGVGANIVSWLILPR 200
           ++  TA + HA   HL  N+F + IFG  +E++ G   + L Y+++G G +I+S L L +
Sbjct: 98  WRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL-Q 157

Query: 201 NAVSVGASGAVFGLFTISVLVKMSWDW---RKILEVLILGQFVIEKVMEAAQASTALSGS 260
            ++SVGASGA+ GL   ++L ++  +W   +  L  L+   F+I     A   +  L   
Sbjct: 158 KSISVGASGALLGLMG-AMLSELLTNWTIYKSKLCALLSFLFII-----AINLAIGL--- 217

Query: 261 FRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIP 291
                 L  V++ AH+ G L G  L ++L   P
Sbjct: 218 ------LPWVDNFAHIGGLLTGFCLGFILLMQP 234

BLAST of MS008407 vs. TAIR 10
Match: AT1G12750.3 (RHOMBOID-like protein 6 )

HSP 1 Score: 53.9 bits (128), Expect = 2.6e-07
Identity = 48/153 (31.37%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 141 YQFFTATFCHANWNHLSSNLFFLYIFGKLVEEEEGNFALWLSYILTGVGANIVSWLILPR 200
           ++  TA + HA   HL  N+F + IFG  +E++ G   + L Y+++G G +I+S L L +
Sbjct: 98  WRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRIGLIYLISGFGGSILSALFL-Q 157

Query: 201 NAVSVGASGAVFGLFTISVLVKMSWDW---RKILEVLILGQFVIEKVMEAAQASTALSGS 260
            ++SVGASGA+ GL   ++L ++  +W   +  L  L+   F+I     A   +  L   
Sbjct: 158 KSISVGASGALLGLMG-AMLSELLTNWTIYKSKLCALLSFLFII-----AINLAIGL--- 217

Query: 261 FRGGYSLQNVNHVAHLSGALVGVVLVWLLSRIP 291
                 L  V++ AH+ G L G  L ++L   P
Sbjct: 218 ------LPWVDNFAHIGGLLTGFCLGFILLMQP 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142618.11.4e-167100.00rhomboid-like protein 11, chloroplastic [Momordica charantia][more]
XP_023524317.12.9e-14186.60rhomboid-like protein 11, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022981184.15.4e-14085.95rhomboid-like protein 11, chloroplastic [Cucurbita maxima][more]
XP_038896989.13.5e-13985.53rhomboid-like protein 11, chloroplastic [Benincasa hispida][more]
XP_022940340.11.7e-13885.02rhomboid-like protein 11, chloroplastic [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q84MB54.7e-10268.15Rhomboid-like protein 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL11... [more]
Q8VZ483.7e-0631.37RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 P... [more]
P966174.8e-0627.40Putative rhomboid protease YdcA OS=Bacillus subtilis (strain 168) OX=224308 GN=y... [more]
Q0WQX72.4e-0534.41RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1[more]
Q9CAN14.1e-0529.68RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CNP96.6e-168100.00rhomboid-like protein 11, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1J1792.6e-14085.95rhomboid-like protein 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111480... [more]
A0A6J1FQ998.4e-13985.02rhomboid-like protein 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5D3E6593.2e-13081.85Rhomboid-like protein 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CQS63.2e-13081.85rhomboid-like protein 11, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G25752.13.3e-10368.15rhomboid-like protein 11 [more]
AT5G25640.14.3e-2667.06Rhomboid-related intramembrane serine protease family protein [more]
AT1G12750.12.6e-0731.37RHOMBOID-like protein 6 [more]
AT1G12750.22.6e-0731.37RHOMBOID-like protein 6 [more]
AT1G12750.32.6e-0731.37RHOMBOID-like protein 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 100..291
e-value: 1.5E-32
score: 114.7
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 104..290
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 137..289
e-value: 2.2E-29
score: 102.4
NoneNo IPR availablePANTHERPTHR43066RHOMBOID-RELATED PROTEINcoord: 43..296
NoneNo IPR availablePANTHERPTHR43066:SF5RHOMBOID-LIKE PROTEIN 11, CHLOROPLASTIC-RELATEDcoord: 43..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008407.1MS008407.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity