MS008405 (gene) Bitter gourd (TR) v1

Overview
NameMS008405
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionATP synthase subunit a, chloroplastic
Locationscaffold4: 1155064 .. 1155336 (+)
RNA-Seq ExpressionMS008405
SyntenyMS008405
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAGAATTAATGAAAACCGAACATAATCATTTAATCAACCCTATCAAGAATATTTATTCACCTCACGGAGAGTTAGCCGCACCCACAAATGATATAAATACTACCGTTGTTTTAGCTTTACTCACATCAATAGCATATTTCTATGCGGGTCTTAGCAAAAAAGGATTAGGTTATTTCGGTAAATACATTCAACCAACTCCAATCCTTTTACCCATTAACATCTTAGAAGATTTCACAAAACCCTTATCACTTAGTTTTCGACTTTTCGGAAAT

mRNA sequence

TTAGAATTAATGAAAACCGAACATAATCATTTAATCAACCCTATCAAGAATATTTATTCACCTCACGGAGAGTTAGCCGCACCCACAAATGATATAAATACTACCGTTGTTTTAGCTTTACTCACATCAATAGCATATTTCTATGCGGGTCTTAGCAAAAAAGGATTAGGTTATTTCGGTAAATACATTCAACCAACTCCAATCCTTTTACCCATTAACATCTTAGAAGATTTCACAAAACCCTTATCACTTAGTTTTCGACTTTTCGGAAAT

Coding sequence (CDS)

TTAGAATTAATGAAAACCGAACATAATCATTTAATCAACCCTATCAAGAATATTTATTCACCTCACGGAGAGTTAGCCGCACCCACAAATGATATAAATACTACCGTTGTTTTAGCTTTACTCACATCAATAGCATATTTCTATGCGGGTCTTAGCAAAAAAGGATTAGGTTATTTCGGTAAATACATTCAACCAACTCCAATCCTTTTACCCATTAACATCTTAGAAGATTTCACAAAACCCTTATCACTTAGTTTTCGACTTTTCGGAAAT

Protein sequence

LELMKTEHNHLINPIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPINILEDFTKPLSLSFRLFGN
Homology
BLAST of MS008405 vs. NCBI nr
Match: QHB74935.1 (ATP synthase CF0 subunit IV [Brunellia trianae] >QHB75022.1 ATP synthase CF0 subunit IV [Brunellia antioquensis])

HSP 1 Score: 148.7 bits (374), Expect = 2.5e-32
Identity = 73/78 (93.59%), Postives = 74/78 (94.87%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I+ PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIHLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. NCBI nr
Match: QXI85908.1 (ATP synthase CF0 subunit IV [Gentiana szechenyii])

HSP 1 Score: 147.9 bits (372), Expect = 4.2e-32
Identity = 71/78 (91.03%), Postives = 74/78 (94.87%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P+K I  PHGELAAPTNDINTTV LALLTS+AYFYAGL+KKGLGYFGKYIQPTPILLPIN
Sbjct: 115 PLKVIQLPHGELAAPTNDINTTVALALLTSVAYFYAGLTKKGLGYFGKYIQPTPILLPIN 174

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 175 ILEDFTKPLSLSFRLFGN 192

BLAST of MS008405 vs. NCBI nr
Match: AFU97004.1 (AtpI, partial [Averrhoa carambola])

HSP 1 Score: 147.5 bits (371), Expect = 5.5e-32
Identity = 73/78 (93.59%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 125 PWKIIQLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 184

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 185 ILEDFTKPLSLSFRLFGN 202

BLAST of MS008405 vs. NCBI nr
Match: QRN71791.1 (ATP synthase CF0 subunit IV [Phanera variegata var. variegata])

HSP 1 Score: 147.5 bits (371), Expect = 5.5e-32
Identity = 73/78 (93.59%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIQLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. NCBI nr
Match: YP_009338618.1 (ATPase subunit IV [Averrhoa carambola] >ANU80142.1 ATPase subunit IV [Averrhoa carambola] >ANY60289.1 ATP synthase CF0 subunit IV [Averrhoa carambola] >QWE50125.1 ATPase subunit IV [Averrhoa carambola])

HSP 1 Score: 147.5 bits (371), Expect = 5.5e-32
Identity = 73/78 (93.59%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIQLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy Swiss-Prot
Match: Q56P09 (ATP synthase subunit a, chloroplastic OS=Lactuca sativa OX=4236 GN=atpI PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 9.5e-35
Identity = 72/78 (92.31%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTS+AYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIQLPHGELAAPTNDINTTVALALLTSVAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy Swiss-Prot
Match: Q14FG9 (ATP synthase subunit a, chloroplastic OS=Populus alba OX=43335 GN=atpI PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 9.5e-35
Identity = 73/78 (93.59%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIELPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy Swiss-Prot
Match: A4GYP6 (ATP synthase subunit a, chloroplastic OS=Populus trichocarpa OX=3694 GN=atpI PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 9.5e-35
Identity = 73/78 (93.59%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIELPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy Swiss-Prot
Match: A7Y3B0 (ATP synthase subunit a, chloroplastic OS=Ipomoea purpurea OX=4121 GN=atpI PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.2e-34
Identity = 72/78 (92.31%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTS+AYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIQLPHGELAAPTNDINTTVALALLTSLAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy Swiss-Prot
Match: Q0G9X4 (ATP synthase subunit a, chloroplastic OS=Daucus carota OX=4039 GN=atpI PE=3 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.6e-34
Identity = 71/78 (91.03%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTS+AYFYAG+SKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIQLPHGELAAPTNDINTTVALALLTSVAYFYAGISKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy TrEMBL
Match: A0A6B9M860 (ATP synthase subunit a, chloroplastic OS=Brunellia trianae OX=2695831 GN=atpI PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.2e-32
Identity = 73/78 (93.59%), Postives = 74/78 (94.87%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I+ PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIHLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy TrEMBL
Match: A0A6B9LZ31 (ATP synthase subunit a, chloroplastic OS=Brunellia antioquensis OX=2695832 GN=atpI PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.2e-32
Identity = 73/78 (93.59%), Postives = 74/78 (94.87%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I+ PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIHLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy TrEMBL
Match: M1JE33 (ATP synthase CF0 subunit IV OS=Alpinia zerumbet OX=97723 GN=atpI PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.7e-32
Identity = 73/78 (93.59%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIQLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy TrEMBL
Match: A0A1B2CXU0 (ATP synthase subunit a, chloroplastic OS=Averrhoa carambola OX=28974 GN=atpI PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.7e-32
Identity = 73/78 (93.59%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIQLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. ExPASy TrEMBL
Match: A0A161CIL3 (ATP synthase subunit a, chloroplastic OS=Senna tora OX=362788 GN=atpI PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.7e-32
Identity = 73/78 (93.59%), Postives = 73/78 (93.59%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  PHGELAAPTNDINTTV LALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN
Sbjct: 116 PWKIIQLPHGELAAPTNDINTTVALALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 175

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 176 ILEDFTKPLSLSFRLFGN 193

BLAST of MS008405 vs. TAIR 10
Match: ATCG00150.1 (ATPase, F0 complex, subunit A protein )

HSP 1 Score: 141.7 bits (356), Expect = 2.8e-34
Identity = 70/78 (89.74%), Postives = 71/78 (91.03%), Query Frame = 0

Query: 14  PIKNIYSPHGELAAPTNDINTTVVLALLTSIAYFYAGLSKKGLGYFGKYIQPTPILLPIN 73
           P K I  P GELAAPTNDINTTV LALLTS+AYFYAGLSKKGLGYF KYIQPTPILLPIN
Sbjct: 118 PWKIIQLPQGELAAPTNDINTTVALALLTSVAYFYAGLSKKGLGYFSKYIQPTPILLPIN 177

Query: 74  ILEDFTKPLSLSFRLFGN 92
           ILEDFTKPLSLSFRLFGN
Sbjct: 178 ILEDFTKPLSLSFRLFGN 195

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
QHB74935.12.5e-3293.59ATP synthase CF0 subunit IV [Brunellia trianae] >QHB75022.1 ATP synthase CF0 sub... [more]
QXI85908.14.2e-3291.03ATP synthase CF0 subunit IV [Gentiana szechenyii][more]
AFU97004.15.5e-3293.59AtpI, partial [Averrhoa carambola][more]
QRN71791.15.5e-3293.59ATP synthase CF0 subunit IV [Phanera variegata var. variegata][more]
YP_009338618.15.5e-3293.59ATPase subunit IV [Averrhoa carambola] >ANU80142.1 ATPase subunit IV [Averrhoa c... [more]
Match NameE-valueIdentityDescription
Q56P099.5e-3592.31ATP synthase subunit a, chloroplastic OS=Lactuca sativa OX=4236 GN=atpI PE=3 SV=... [more]
Q14FG99.5e-3593.59ATP synthase subunit a, chloroplastic OS=Populus alba OX=43335 GN=atpI PE=3 SV=1[more]
A4GYP69.5e-3593.59ATP synthase subunit a, chloroplastic OS=Populus trichocarpa OX=3694 GN=atpI PE=... [more]
A7Y3B01.2e-3492.31ATP synthase subunit a, chloroplastic OS=Ipomoea purpurea OX=4121 GN=atpI PE=3 S... [more]
Q0G9X41.6e-3491.03ATP synthase subunit a, chloroplastic OS=Daucus carota OX=4039 GN=atpI PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6B9M8601.2e-3293.59ATP synthase subunit a, chloroplastic OS=Brunellia trianae OX=2695831 GN=atpI PE... [more]
A0A6B9LZ311.2e-3293.59ATP synthase subunit a, chloroplastic OS=Brunellia antioquensis OX=2695832 GN=at... [more]
M1JE332.7e-3293.59ATP synthase CF0 subunit IV OS=Alpinia zerumbet OX=97723 GN=atpI PE=3 SV=1[more]
A0A1B2CXU02.7e-3293.59ATP synthase subunit a, chloroplastic OS=Averrhoa carambola OX=28974 GN=atpI PE=... [more]
A0A161CIL32.7e-3293.59ATP synthase subunit a, chloroplastic OS=Senna tora OX=362788 GN=atpI PE=3 SV=1[more]
Match NameE-valueIdentityDescription
ATCG00150.12.8e-3489.74ATPase, F0 complex, subunit A protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 89..91
NoneNo IPR availableCDDcd00310ATP-synt_Fo_a_6coord: 25..91
e-value: 2.84106E-18
score: 72.0468
IPR035908ATP synthase, F0 complex, subunit A superfamilyGENE3D1.20.120.220ATP synthase, F0 complex, subunit Acoord: 2..91
e-value: 1.4E-16
score: 63.1
IPR035908ATP synthase, F0 complex, subunit A superfamilySUPERFAMILY81336F1F0 ATP synthase subunit Acoord: 19..91
IPR000568ATP synthase, F0 complex, subunit APFAMPF00119ATP-synt_Acoord: 25..91
e-value: 1.8E-18
score: 67.2
IPR045082ATP synthase, F0 complex, subunit A, bacterial/chloroplastPANTHERPTHR42823ATP SYNTHASE SUBUNIT A, CHLOROPLASTICcoord: 11..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008405.1MS008405.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015986 ATP synthesis coupled proton transport
cellular_component GO:0045263 proton-transporting ATP synthase complex, coupling factor F(o)
molecular_function GO:0015078 proton transmembrane transporter activity