MS008352 (gene) Bitter gourd (TR) v1

Overview
NameMS008352
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionArgininosuccinate lyase
Locationscaffold4: 757082 .. 760733 (+)
RNA-Seq ExpressionMS008352
SyntenyMS008352
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCCTTGGGCAGCTACTCTTTACCCACCGCAACCGCATTTGCCTTCTCTAAACCCTCCGTTTCTGCTTCTAAGCCGCCATTTCTCGCCTTCTCTCGTCGAGCCATGCCGGTGTTTGGGGTTCTCAGTGCATCTCGCACCGCCGACGCGGCGCCGTCCGTCGCCGACTCGCCGGACAATCACGCCGACAAGGAGGTCAAGCTTTGGGGCGGCCGTTTCGAGGAGAGCGTCACCGACGCAGTTGAGCGATTTACCGAGTCGATCTCCTACGATAAGCAGCTTTATAAGCACGATATCAGGGGGAGTCGAGCTCATGCTTCTATGCTAGCTAAGCAGGTTTGTTTTGATTTCTCGCCTTCGTCAGAATGTGTTCGTTCTGTTCATTTCTCTCTTTTTGATTGTTGCATTTCATTTGATTCCACTACTATCTGGAAAAAGAATACAAATGCGATTGATTTCACCTGTTTTAGGTAATTTTGTTGCCTTGTCATTGCTGTTTCATATGTTGAAGTAAGTTGATTCAGGAATGCGAACACATTTGAACTCCTTAAGCTTATTGTAGTTTATCAGTTGGCTTGTTAATTGAATCTTTGAGTATTTTCCTTTTCGCTTCTTTAATGAATTGAAACTTATTACTTTAGGGACTGATGAGTGTTGATGAGAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAGGCGGATTGAAAGTGGTGAGTTTGTTTGGAGAACAGATAGGGAGGATGTTCACATGAACATTGAGGCTGCCCTGATTGATATGATTGGTGAACCTGCAAAGAAGCTGCATACTGCTCGGAGCCGCAATGACCAAGTTTTGACTGATTTTCGATTGTGGTGTCGGGACGCAATTGATAAGATATTAGATGCTATTAAGTACCTTCAGGTTTCCATGGTAACTCTGGCATTGTCGAATGAGGGATTAATCGTTCCGGGTTATACGCATTTACAAAGAGCACAACCCGTTCTTCTCCAGCATCTGCTCTTAGCATTTGTTGAGCAGGTAAGGTTCTTCTAATGAAATTACTCTGTTTTTATTTGCCTAAACCAAAGAGCTTATTGTTTACAACTATTAGGATAAGCAAAGAGTGATGTGGGGGTAAATAGACAACATCTACATGCATGAATATTTTTTATTCATTGCGATGGTGCTAGCAGTAGCACTATGCTACAACTTGATGGGGCTAACTCTAGCCTAAAAGAAAATAGAAAATTCTTAAGGCTTGAACTTGTTAGGATTATTACATAAAGCATAAGCTGCATAACAATCAACCTTACTCATGGATTCTTAAATAAAATGTCGTTCTAGGCAGTTTATTTATTCTCTCTAAAATTAAAATCTCTTGAAGACTTGATTGTTTTAAAATCATTGTTTTATAGCTTGAAAGAGATGCTGGTCGTTTATCGGATTGCAGAGTTAGGCTAAATTTTTGCCCACTAGGTGCATGTGCGCTGGCTGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGATGCTTTAGGGTTCACTGCCCCCTTGAGAAACAGGTACGAAACTTCATTTCTTTCTCTCTTATTATTATTATTATTATTTATTTATTTATTTTTGGTAAGAAATGGATATTTCATTGATGATATGAAATTTACAAAAAGGATTACAAAAAAGGCCTTCAAGCTTGTCAAGAGATCATTAAGACTATAATTACTAATTAGTAAAAGGGTAATAGAAACATCTACACCAACCAAGAGATACAACCATAACTCTGTCAAAAAAAATCTTATTTGAAAGCTATAGAAGTTACTAAAGATGGTGTTTTTATTTGATATATTATTGAAAGTTGTTTTATATATGTATGTGTCTATGGTAGTCTATGGTAGTTTATAATATATCAGTGTGCCAATTTCTTTTTGTCTTTGCTTTCTTGTTCCAAATTGTTCTCAGGTTGTTAAGAAGCTGACTAAAAAATTTTCATACACACACACATATTGCCGCATTTATATATATAGCTCTATTATCTCCTACACTTTGATAAATAATTTTGGCCATAAAACAGCTTTGGAATGAGACCAATTTCCATATTAGGAAGTAAAAAATCTTCAGCTGATCCTGCTTTCACTTTGCTTTAGGGAATTTTAATGACTTTCTTTCCCACATCTGGTTTTGTCAAATCAGTAGTAATTTATAATGTTTGATTATCTTTACAGCATTGATGCAGTTTCCGACCGAGATTTTGCCTTAGAGTTTCTTTCTGCCAATGCCATTACTGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAGTTTGGATTTATAATGCCAAATGATTCCGTTTCGACCGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTTGTTCGTGGGAAGTCAGCTAGAGTCATTGGAGACTTGGTCACCACTCTCACCTTATGCAAAGGTCTGCCTCTGGCCTACAATCGTGATTTGCAGGTAAAATCTTTTTACGTTTTGTTTTGACCTTGACCATTTGTTATATGTAGGAGAAGGGAACATTTGCAAAGCAAATTTTCTTGTGATTTTAATTCCTCGGTGAATGCTTCGTTTTCTTAATGATGTGGTTTTTAATACGAAAAACGTTCCTATATTGAATAGGAAGACAAGGAACCTGTATTTGACAGTGTAAAAACAATATTGGGAATGCTTGAAGTGTCTGCTGAGTTTGCTCAGAATATTTCTTACAATCGGGAGAGAATACGGAAATCTTTACCTGCCGGTTTTCTTGATGCCACAACTCTTGCTGATTACCTCGTGAAGAAGGTTGGTTTGACTTGCAAATATCTTTTTTTCCTCCTTCTAGGCTACTTGGTGAGAAGCATCGCTAGTGATCTTTCTCGCCATCTATATCCTTTAGAATCCTGAACACATTTCATTTATAAGAATACTTGAAAACGAATAAAAAGTTTTACTATTCTTTTTTTGAATAAGAAAAAGGTGATATATAGAAGGAAAAGAACAGCCTAAAGGCGAGGGGAAGAGAAGGCCCTGCCTCACAAAAACTAAAGAATGATAACTTTTCAATAGACTGTAGTTACAAAAGAAAACCTTGTGGTTTAAGCACCAAGTTAGAAGCAGTAATGTGTACACAATTACAATTCTCAACCACCATGGATTAGTGGATTGCCATAGTGACACCAGAGGTCGTGACGTTTGGGGAAATGGGTTCAAACGGTAGCCATCTATCTAGGATTTTAAAATCTTATGAGTTTCTTCACAACTTAACGTTGAAAGGTGAACGGCTGTCCCATGAGACTAGTTAAATGACCTTGTTTTTCATCTTCTAAGAAAAGAAACGAGAAACTAACATCAAATTTATGGCCTGTTTTACTGTATCAGGGAATACCTTTCAGAACAGGTCACGACATAGTTGGGAAGTGTGTGGCCTTGTGTGTCACAAAGAATTGCTGCCTCCAGGACTTGAGTCTCGACGAGCTAAGAAGCATAAGTCCAGTATTCGAAGCGGATGTTTACGAGTTTCTCGGCCCCGAGAATGCCATTAATAAATTTTGTTCATATGGTTCCACAGGAGCAGCCTGTGTTGCTACTCAACTCGAATACTGGGTTAATAAACTCCAAATCGATCAG

mRNA sequence

ATGGAGGCCTTGGGCAGCTACTCTTTACCCACCGCAACCGCATTTGCCTTCTCTAAACCCTCCGTTTCTGCTTCTAAGCCGCCATTTCTCGCCTTCTCTCGTCGAGCCATGCCGGTGTTTGGGGTTCTCAGTGCATCTCGCACCGCCGACGCGGCGCCGTCCGTCGCCGACTCGCCGGACAATCACGCCGACAAGGAGGTCAAGCTTTGGGGCGGCCGTTTCGAGGAGAGCGTCACCGACGCAGTTGAGCGATTTACCGAGTCGATCTCCTACGATAAGCAGCTTTATAAGCACGATATCAGGGGGAGTCGAGCTCATGCTTCTATGCTAGCTAAGCAGGGACTGATGAGTGTTGATGAGAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAGGCGGATTGAAAGTGGTGAGTTTGTTTGGAGAACAGATAGGGAGGATGTTCACATGAACATTGAGGCTGCCCTGATTGATATGATTGGTGAACCTGCAAAGAAGCTGCATACTGCTCGGAGCCGCAATGACCAAGTTTTGACTGATTTTCGATTGTGGTGTCGGGACGCAATTGATAAGATATTAGATGCTATTAAGTACCTTCAGGTTTCCATGGTAACTCTGGCATTGTCGAATGAGGGATTAATCGTTCCGGGTTATACGCATTTACAAAGAGCACAACCCGTTCTTCTCCAGCATCTGCTCTTAGCATTTGTTGAGCAGCTTGAAAGAGATGCTGGTCGTTTATCGGATTGCAGAGTTAGGCTAAATTTTTGCCCACTAGGTGCATGTGCGCTGGCTGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGATGCTTTAGGGTTCACTGCCCCCTTGAGAAACAGCATTGATGCAGTTTCCGACCGAGATTTTGCCTTAGAGTTTCTTTCTGCCAATGCCATTACTGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAGTTTGGATTTATAATGCCAAATGATTCCGTTTCGACCGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTTGTTCGTGGGAAGTCAGCTAGAGTCATTGGAGACTTGGTCACCACTCTCACCTTATGCAAAGGTCTGCCTCTGGCCTACAATCGTGATTTGCAGGAAGACAAGGAACCTGTATTTGACAGTGTAAAAACAATATTGGGAATGCTTGAAGTGTCTGCTGAGTTTGCTCAGAATATTTCTTACAATCGGGAGAGAATACGGAAATCTTTACCTGCCGGTTTTCTTGATGCCACAACTCTTGCTGATTACCTCGTGAAGAAGGGAATACCTTTCAGAACAGGTCACGACATAGTTGGGAAGTGTGTGGCCTTGTGTGTCACAAAGAATTGCTGCCTCCAGGACTTGAGTCTCGACGAGCTAAGAAGCATAAGTCCAGTATTCGAAGCGGATGTTTACGAGTTTCTCGGCCCCGAGAATGCCATTAATAAATTTTGTTCATATGGTTCCACAGGAGCAGCCTGTGTTGCTACTCAACTCGAATACTGGGTTAATAAACTCCAAATCGATCAG

Coding sequence (CDS)

ATGGAGGCCTTGGGCAGCTACTCTTTACCCACCGCAACCGCATTTGCCTTCTCTAAACCCTCCGTTTCTGCTTCTAAGCCGCCATTTCTCGCCTTCTCTCGTCGAGCCATGCCGGTGTTTGGGGTTCTCAGTGCATCTCGCACCGCCGACGCGGCGCCGTCCGTCGCCGACTCGCCGGACAATCACGCCGACAAGGAGGTCAAGCTTTGGGGCGGCCGTTTCGAGGAGAGCGTCACCGACGCAGTTGAGCGATTTACCGAGTCGATCTCCTACGATAAGCAGCTTTATAAGCACGATATCAGGGGGAGTCGAGCTCATGCTTCTATGCTAGCTAAGCAGGGACTGATGAGTGTTGATGAGAGGGACAGTATTCTTGAAGGTTTGGATGAAATTGAGAGGCGGATTGAAAGTGGTGAGTTTGTTTGGAGAACAGATAGGGAGGATGTTCACATGAACATTGAGGCTGCCCTGATTGATATGATTGGTGAACCTGCAAAGAAGCTGCATACTGCTCGGAGCCGCAATGACCAAGTTTTGACTGATTTTCGATTGTGGTGTCGGGACGCAATTGATAAGATATTAGATGCTATTAAGTACCTTCAGGTTTCCATGGTAACTCTGGCATTGTCGAATGAGGGATTAATCGTTCCGGGTTATACGCATTTACAAAGAGCACAACCCGTTCTTCTCCAGCATCTGCTCTTAGCATTTGTTGAGCAGCTTGAAAGAGATGCTGGTCGTTTATCGGATTGCAGAGTTAGGCTAAATTTTTGCCCACTAGGTGCATGTGCGCTGGCTGGCACAGGCCTTCCAATTGATCGTTTTATGACTGCTGATGCTTTAGGGTTCACTGCCCCCTTGAGAAACAGCATTGATGCAGTTTCCGACCGAGATTTTGCCTTAGAGTTTCTTTCTGCCAATGCCATTACTGCTATTCATCTTTCAAGGCTTGGAGAAGAATGGGTGTTGTGGGCTTCTGAAGAGTTTGGATTTATAATGCCAAATGATTCCGTTTCGACCGGAAGTAGCATAATGCCTCAGAAGAAAAATCCAGATCCAATGGAACTTGTTCGTGGGAAGTCAGCTAGAGTCATTGGAGACTTGGTCACCACTCTCACCTTATGCAAAGGTCTGCCTCTGGCCTACAATCGTGATTTGCAGGAAGACAAGGAACCTGTATTTGACAGTGTAAAAACAATATTGGGAATGCTTGAAGTGTCTGCTGAGTTTGCTCAGAATATTTCTTACAATCGGGAGAGAATACGGAAATCTTTACCTGCCGGTTTTCTTGATGCCACAACTCTTGCTGATTACCTCGTGAAGAAGGGAATACCTTTCAGAACAGGTCACGACATAGTTGGGAAGTGTGTGGCCTTGTGTGTCACAAAGAATTGCTGCCTCCAGGACTTGAGTCTCGACGAGCTAAGAAGCATAAGTCCAGTATTCGAAGCGGATGTTTACGAGTTTCTCGGCCCCGAGAATGCCATTAATAAATTTTGTTCATATGGTTCCACAGGAGCAGCCTGTGTTGCTACTCAACTCGAATACTGGGTTAATAAACTCCAAATCGATCAG

Protein sequence

MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPDNHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDERDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRERIRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISPVFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQIDQ
Homology
BLAST of MS008352 vs. NCBI nr
Match: XP_022142042.1 (argininosuccinate lyase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.1e-300
Identity = 521/525 (99.24%), Postives = 524/525 (99.81%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           MEALGSYSLPTATAFAFSKPSVS+SKPPFL FSRRAMPVFGVLSASRTADAAPSVADSPD
Sbjct: 1   MEALGSYSLPTATAFAFSKPSVSSSKPPFLTFSRRAMPVFGVLSASRTADAAPSVADSPD 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE
Sbjct: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLN+CPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNYCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           IRK+LPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP
Sbjct: 421 IRKALPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQIDQ 526
           VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQIDQ
Sbjct: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQIDQ 525

BLAST of MS008352 vs. NCBI nr
Match: XP_008466162.1 (PREDICTED: argininosuccinate lyase, chloroplastic [Cucumis melo] >KAA0038666.1 argininosuccinate lyase [Cucumis melo var. makuwa] >TYK31276.1 argininosuccinate lyase [Cucumis melo var. makuwa])

HSP 1 Score: 964.9 bits (2493), Expect = 2.8e-277
Identity = 485/524 (92.56%), Postives = 500/524 (95.42%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           M+ L SYSLP   AFAFSKPSVS SKPPFLA +R AMP+FGVL AS TA+A PSV DSP+
Sbjct: 1   MDCLTSYSLP--AAFAFSKPSVSLSKPPFLAVARGAMPMFGVLRASHTANAPPSVYDSPE 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           N  DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLAKQGLMSVDE
Sbjct: 61  NRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSILEGLD+IER+IESG+FVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAID ILDAIKYLQVSMVTLAL NEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLNF PLGACALAGTGLPIDRFMTA+ALGFT PLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFI PNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNIS+NRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           I K+LPAG+LDATTLADYLVKKGIPFRTGHDIVGK VALCVTK CCLQDLSLDELRSISP
Sbjct: 421 IEKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKKCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQID 525
           VFE DVYEFLG ENAINKFCSYGSTGAACVATQ+EYWVNKLQI+
Sbjct: 481 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIN 522

BLAST of MS008352 vs. NCBI nr
Match: XP_038900244.1 (argininosuccinate lyase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 961.8 bits (2485), Expect = 2.4e-276
Identity = 482/524 (91.98%), Postives = 499/524 (95.23%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           M+ L SYSLP   A AFSKPSVSASKPPFLA  R A+PVFGVL ASRTADA PSV DSP+
Sbjct: 1   MDCLTSYSLP--AALAFSKPSVSASKPPFLAVVRGAVPVFGVLRASRTADAPPSVEDSPE 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           N  DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLAKQGLMSV E
Sbjct: 61  NRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVHE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSILEGLD+IER+IESG+FVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSILEGLDKIERQIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAID ILDA+KYLQVSMVTLAL NEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDTILDAMKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLN CPLGACALAGTGLPIDRFMTA+ALGFTAPLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAI AIHLSRLGEEWVLWASEEFGFI PNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAIAAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNIS+NRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           I K+LPAG+LDATTLADYLVKKGIPFRTGHDIVGK VALCVTKNCCLQDLSLDELRSISP
Sbjct: 421 IEKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKNCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQID 525
           VF+ DVYEFLG ENAINKFCSYGSTG ACVATQ+EYW+NKL+I+
Sbjct: 481 VFDEDVYEFLGAENAINKFCSYGSTGKACVATQIEYWINKLEIN 522

BLAST of MS008352 vs. NCBI nr
Match: XP_023536382.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 951.4 bits (2458), Expect = 3.2e-273
Identity = 481/524 (91.79%), Postives = 495/524 (94.47%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           M+ L SY LP     AFSK SVSASK PF A +R A+PVFGVL ASRTADA  SV DSP+
Sbjct: 1   MDCLTSYFLP--ATLAFSKSSVSASKLPFFAAARGALPVFGVLRASRTADAPLSVDDSPE 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           N  DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLA QGLMSV E
Sbjct: 61  NRDDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLASQGLMSVAE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSI+EGLDEIERRIESG+FVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAID ILDAI+YLQVSMVTLAL NEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLN CPLGACALAGTGLPIDRFMTA+ALGFTAPLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFI PNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNIS+NRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           I K+LPAG+LDATTLADYLVKKGIPFRTGHDIVGK VALCVTK+CCLQDLSLDELRSISP
Sbjct: 421 IEKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKSCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQID 525
           VFE DVYEFLG ENAINKFCSYGSTGAACVATQ+EYWVNKLQI+
Sbjct: 481 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQMEYWVNKLQIN 522

BLAST of MS008352 vs. NCBI nr
Match: XP_022976892.1 (argininosuccinate lyase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 950.3 bits (2455), Expect = 7.1e-273
Identity = 480/524 (91.60%), Postives = 495/524 (94.47%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           M+ L SY LP     AFSK SVSASK PF A +R A+PVFGVL ASRTADA  SV D P+
Sbjct: 1   MDCLTSYFLP--ATLAFSKSSVSASKLPFFAVARGALPVFGVLRASRTADAPLSVDDWPE 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           N +DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLA QGLMSV E
Sbjct: 61  NRSDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLANQGLMSVAE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSI+EGLDEIERRIESG+FVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAID ILDAI+YLQVSMVTLAL NEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLN CPLGACALAGTGLPIDRFMTA+ALGFTAPLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFI PNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNIS+NRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           I K+LPAG+LDATTLADYLVKKGIPFRTGHDIVGK VALCVTK+CCLQDLSLDELRSISP
Sbjct: 421 IEKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKSCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQID 525
           VFE DVYEFLG ENAINKFCSYGSTGAACVATQ+EYWVNKLQI+
Sbjct: 481 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIN 522

BLAST of MS008352 vs. ExPASy Swiss-Prot
Match: Q9LEU8 (Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10920 PE=2 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 4.1e-215
Identity = 372/501 (74.25%), Postives = 425/501 (84.83%), Query Frame = 0

Query: 23  SASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPDNHADKEVKLWGGRFEESVTDAV 82
           S+S+    + + R++      S SR      S++   D+ A KEVKLWGGRFEESVT+ V
Sbjct: 15  SSSRSNLSSSTHRSVSFLPPGSKSRCLPPLRSMSHD-DDTASKEVKLWGGRFEESVTEKV 74

Query: 83  ERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDERDSILEGLDEIERRIESGEFVW 142
           E+FTESIS+DK LYK DI GS+AHASMLA QGL++  ++DSIL GLD+IER+IE+ +F W
Sbjct: 75  EKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSDKDSILRGLDDIERQIEANKFEW 134

Query: 143 RTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDKILDAIKYLQV 202
           RTDREDVHMNIEAAL D+IGEPAKKLHTARSRNDQV TDFRLWCRDAID I+  I+ LQ 
Sbjct: 135 RTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDTIIVKIRNLQR 194

Query: 203 SMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNFCPLGA 262
           ++V LAL NE LIVPGYTHLQRAQPVLL H+LL FVEQLERDAGR  DCR RLNF PLGA
Sbjct: 195 ALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQLERDAGRYVDCRARLNFSPLGA 254

Query: 263 CALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWV 322
           CALAGTGLPIDRFMTA+ALGFT P+RNSIDAVSDRDF LEFL  NA T IHLSRLGEEWV
Sbjct: 255 CALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFVLEFLYTNANTGIHLSRLGEEWV 314

Query: 323 LWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAY 382
           LWASEEFGF+ P+DSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVT LTLCKGLPLAY
Sbjct: 315 LWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTVLTLCKGLPLAY 374

Query: 383 NRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRERIRKSLPAGFLDATTLADYLVKK 442
           NRD QEDKEP+FDS KTI+GM++VSAEFAQN+++N +RI+KSLPAG LDATTLADYLVKK
Sbjct: 375 NRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDRIKKSLPAGHLDATTLADYLVKK 434

Query: 443 GIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISPVFEADVYEFLGPENAINKFCSY 502
           G+PFR+ HDIVGK V +CV+K C LQ+LSL+E++ +SPVFE DV+ FLG EN++NKF SY
Sbjct: 435 GMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSPVFEEDVFGFLGVENSVNKFSSY 494

Query: 503 GSTGAACVATQLEYWVNKLQI 524
           GSTG+ CVA QL YWVNKL I
Sbjct: 495 GSTGSNCVAEQLGYWVNKLNI 514

BLAST of MS008352 vs. ExPASy Swiss-Prot
Match: Q6AR60 (Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) OX=177439 GN=argH PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 8.2e-131
Identity = 228/450 (50.67%), Postives = 303/450 (67.33%), Query Frame = 0

Query: 65  KEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDERDSI 124
           K  KLWGGRF E++  +VE+F+ESISYD +LYK+DI GS+AHA+ML+ QG++S +E + I
Sbjct: 5   KSKKLWGGRFSEAMAASVEKFSESISYDVRLYKYDIAGSKAHATMLSSQGIISPEELEQI 64

Query: 125 LEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRL 184
           + GL  IE  IE+G F ++T+ EDVHMNIE AL+D IG    +LH ARSRNDQ+  DF++
Sbjct: 65  IAGLSSIEADIEAGVFEFKTEYEDVHMNIEQALVDRIGAAGSRLHAARSRNDQIALDFKM 124

Query: 185 WCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQLERD 244
           + RD  D +++ +     +   +     G I+PGYTH QRAQPVL+ H +LA+ E   RD
Sbjct: 125 YLRDQCDHLVELLDGACRAFTVVGRKYLGDIMPGYTHTQRAQPVLITHHMLAYYEMFRRD 184

Query: 245 AGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFALEFL 304
             R+ DCR RLN  PLG  A+AGTGLPI+R   A ALGF     NS+D  +DRD+A+E  
Sbjct: 185 RDRILDCRKRLNLSPLGCAAMAGTGLPINREQVAKALGFAGVTANSMDTSADRDYAIELT 244

Query: 305 SANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGKSARV 364
           S   +  +HLSRL EE V W++ E+ F+  +DS  TGSSIMPQKKNPD  EL+RGKS RV
Sbjct: 245 SCLTMIQLHLSRLAEELVTWSTSEYKFVDISDSFCTGSSIMPQKKNPDIAELIRGKSGRV 304

Query: 365 IGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRERIRKS 424
           +G L++ +T+ KGLPL YNRD QEDKEPVFD++ T+   L ++AE   ++ +N  R  ++
Sbjct: 305 VGSLISLITMMKGLPLTYNRDQQEDKEPVFDAIDTVSASLSITAEMMAHMKFNTARCAEA 364

Query: 425 LPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISPVFEA 484
              GF+ AT LADYLV K +PFR  H IVG  VA C+ K C L DL+L E++  SPV E+
Sbjct: 365 TETGFITATDLADYLVMKDVPFRQAHHIVGSAVAACIAKGCELPDLTLTEMQEFSPVIES 424

Query: 485 DVYEFLGPENAINKFCSYGSTGAACVATQL 515
           DV+  L  E ++N   S G TG   V   L
Sbjct: 425 DVFAVLTAEGSVNSRVSTGGTGLVRVTEAL 454

BLAST of MS008352 vs. ExPASy Swiss-Prot
Match: B8GSV2 (Argininosuccinate lyase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=396588 GN=argH PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.4e-130
Identity = 238/447 (53.24%), Postives = 303/447 (67.79%), Query Frame = 0

Query: 68  KLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDERDSILEG 127
           KLWGGRF E     VE FT S+S+D++LY+HDI+GS+AHA MLA+ G+++ +E D I +G
Sbjct: 9   KLWGGRFSEPTDAFVEAFTASVSFDQRLYRHDIQGSKAHARMLARVGVLTEEECDKIHDG 68

Query: 128 LDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCR 187
           LD I   IE G+F W  + EDVHMN+EA LI+ IG+  KKLHT RSRNDQV TD RL+ R
Sbjct: 69  LDAILADIERGDFAWSVELEDVHMNVEARLIERIGDVGKKLHTGRSRNDQVATDIRLYLR 128

Query: 188 DAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGR 247
           +AID ++  I+ LQ  +V LA      I+PG+THLQ AQPV   H ++A+ E LERD GR
Sbjct: 129 EAIDALMQEIRRLQGGLVDLAEREAQTIMPGFTHLQTAQPVTFGHHMMAWYEMLERDHGR 188

Query: 248 LSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFALEFLSAN 307
           L+DCR R+N  PLGA ALAGT  P+DR  TA+ LGF A   NS+DAVSDRDFA+EF +A 
Sbjct: 189 LADCRKRVNVLPLGAAALAGTPYPLDRHYTAELLGFDAVTDNSLDAVSDRDFAIEFCAAG 248

Query: 308 AITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGD 367
           A+   HLSR  EE +LWAS +FGF+   D   TGSSIMPQKKNPD  ELVRGK+ R  G+
Sbjct: 249 ALIMTHLSRFSEELILWASAQFGFVDLPDRFCTGSSIMPQKKNPDVPELVRGKTGRANGN 308

Query: 368 LVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRERIRKSLPA 427
           L+  LTL KG PLAYN+D QEDKEP+FD+V T+ G L   A+   +I    E +R +   
Sbjct: 309 LIALLTLMKGQPLAYNKDNQEDKEPLFDTVDTLAGSLRAFADMVPHIQVRAEVMRAAASR 368

Query: 428 GFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISPVFEADVY 487
           GF  AT LADYLV++G+PFR  H++VG+ V   V     L ++SLDELR  S   E DV+
Sbjct: 369 GFATATDLADYLVRRGMPFRDAHEVVGRAVRHGVETGKDLAEMSLDELRKFSKTIEQDVF 428

Query: 488 EFLGPENAINKFCSYGSTGAACVATQL 515
           E L  E ++     +G T  A V  ++
Sbjct: 429 EVLTLEGSVAARNVHGGTAPAQVRARI 455

BLAST of MS008352 vs. ExPASy Swiss-Prot
Match: Q67S72 (Argininosuccinate lyase OS=Symbiobacterium thermophilum (strain T / IAM 14863) OX=292459 GN=argH PE=3 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 7.7e-129
Identity = 231/447 (51.68%), Postives = 305/447 (68.23%), Query Frame = 0

Query: 68  KLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDERDSILEG 127
           KLWGGRF +      E FT S+++D++LYK DIRGS AH  ML +QG++   +   I +G
Sbjct: 3   KLWGGRFTKPADKTAEGFTSSLAFDRRLYKQDIRGSIAHVRMLGRQGIIPAADAARIEQG 62

Query: 128 LDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCR 187
           L EIE  IE+G+F +R + ED+H+NIE  LI+ IG    +LHTARSRNDQV+TD  LW +
Sbjct: 63  LREIEAEIEAGQFPFRQEYEDIHLNIEKRLIEKIGPAGGRLHTARSRNDQVVTDVHLWVK 122

Query: 188 DAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGR 247
           D I  +   +  LQ +++  A    G ++PGYTHLQRAQPVLL H L+A+   LERD GR
Sbjct: 123 DEIAAVQRLVSDLQGTLLDRAREQMGAVMPGYTHLQRAQPVLLSHHLMAYFWMLERDYGR 182

Query: 248 LSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFALEFLSAN 307
            +D   R +  PLGA ALAGT  PIDR  TA  LGF     NS+DAVSDRDF +EF++A 
Sbjct: 183 FADALRRADVSPLGAGALAGTTFPIDREFTAAELGFAGVYPNSMDAVSDRDFIVEFVAAA 242

Query: 308 AITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGD 367
           AI  +HLSRL EE V+W+S EFGF+  +D+ +TGSSIMPQKKNPD  ELVRGK+ R+ GD
Sbjct: 243 AICQMHLSRLAEELVMWSSTEFGFVEMDDAYATGSSIMPQKKNPDVAELVRGKTGRIYGD 302

Query: 368 LVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRERIRKSLPA 427
           L+  LT+ KGLPLAY+ DLQEDKE +FD+V T+   L V       + +NRER+ +++  
Sbjct: 303 LMALLTVLKGLPLAYHTDLQEDKERLFDAVDTLKACLTVMTGMLATLKFNRERMAQAVRR 362

Query: 428 GFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISPVFEADVY 487
            F +AT +ADYLVKKG+PFR  H++VGK V  CV +   L DL+L+E ++ S +FEAD+Y
Sbjct: 363 DFSNATDMADYLVKKGMPFREAHEVVGKAVLYCVERGKFLADLTLEEFKAFSALFEADIY 422

Query: 488 EFLGPENAINKFCSYGSTGAACVATQL 515
           + + PE  +++  S G T  A V  QL
Sbjct: 423 QAIAPETCVSQRTSLGGTAPAEVERQL 449

BLAST of MS008352 vs. ExPASy Swiss-Prot
Match: Q2SQ67 (Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=argH PE=3 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 6.5e-128
Identity = 236/442 (53.39%), Postives = 293/442 (66.29%), Query Frame = 0

Query: 64  DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDERDS 123
           D   KLWGGRF E+    VERFT S+ +D+++Y HDIRGS AHA+MLAK G+++ +ERD 
Sbjct: 5   DTTDKLWGGRFTEATDAFVERFTASVDFDRRMYHHDIRGSIAHATMLAKVGVLTEEERDQ 64

Query: 124 ILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFR 183
           I+ GL EIE  IE+G F W    EDVHMNIEA L   IG   KKLHT RSRNDQV TD R
Sbjct: 65  IINGLREIEAEIEAGAFEWSVKLEDVHMNIEARLTQKIGITGKKLHTGRSRNDQVATDIR 124

Query: 184 LWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQLER 243
           L+ RD +D I   +  L  ++  LA      I+PG+THLQ AQPV   H +LA+ E L+R
Sbjct: 125 LYLRDEVDVIAAELLRLIAALADLAEREADAIMPGFTHLQTAQPVTFGHHMLAWAEMLKR 184

Query: 244 DAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFALEF 303
           D  RL+DCR R N  PLGA ALAGT   IDR  T++ LGF  P  NS+DAVSDRDFA+EF
Sbjct: 185 DFSRLADCRERFNVSPLGAAALAGTTYSIDRQYTSELLGFNGPAENSLDAVSDRDFAIEF 244

Query: 304 LSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGKSAR 363
            S  ++  +HLSR  EE VLW S +F FI   D   TGSSIMPQKKNPD  EL+RGKS R
Sbjct: 245 CSFASLLMMHLSRFSEELVLWTSAQFNFINLPDRFCTGSSIMPQKKNPDVPELIRGKSGR 304

Query: 364 VIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRERIRK 423
           V G L++ L L K  PLAYN+D QEDKEP+FD++ T+ G L+   +    +S NR+ + +
Sbjct: 305 VSGHLISLLMLMKSQPLAYNKDNQEDKEPLFDAIDTVKGCLKAYGDMMPAVSVNRDAMAE 364

Query: 424 SLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISPVFE 483
           S   GF  AT LADYLV+KG+PFR  H++VGK VAL V+    L ++SL+ELR  S   E
Sbjct: 365 SARRGFSTATDLADYLVRKGLPFRDAHEVVGKAVALGVSSGRDLSEMSLEELREFSADIE 424

Query: 484 ADVYEFLGPENAINKFCSYGST 506
            DV+E L  E ++N     G T
Sbjct: 425 VDVFEVLTLEGSVNARNHIGGT 446

BLAST of MS008352 vs. ExPASy TrEMBL
Match: A0A6J1CLI1 (argininosuccinate lyase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111012263 PE=3 SV=1)

HSP 1 Score: 1041.2 bits (2691), Expect = 1.5e-300
Identity = 521/525 (99.24%), Postives = 524/525 (99.81%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           MEALGSYSLPTATAFAFSKPSVS+SKPPFL FSRRAMPVFGVLSASRTADAAPSVADSPD
Sbjct: 1   MEALGSYSLPTATAFAFSKPSVSSSKPPFLTFSRRAMPVFGVLSASRTADAAPSVADSPD 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE
Sbjct: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLN+CPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNYCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           IRK+LPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP
Sbjct: 421 IRKALPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQIDQ 526
           VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQIDQ
Sbjct: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQIDQ 525

BLAST of MS008352 vs. ExPASy TrEMBL
Match: A0A5A7T8Y2 (Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005690 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.3e-277
Identity = 485/524 (92.56%), Postives = 500/524 (95.42%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           M+ L SYSLP   AFAFSKPSVS SKPPFLA +R AMP+FGVL AS TA+A PSV DSP+
Sbjct: 1   MDCLTSYSLP--AAFAFSKPSVSLSKPPFLAVARGAMPMFGVLRASHTANAPPSVYDSPE 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           N  DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLAKQGLMSVDE
Sbjct: 61  NRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSILEGLD+IER+IESG+FVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAID ILDAIKYLQVSMVTLAL NEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLNF PLGACALAGTGLPIDRFMTA+ALGFT PLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFI PNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNIS+NRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           I K+LPAG+LDATTLADYLVKKGIPFRTGHDIVGK VALCVTK CCLQDLSLDELRSISP
Sbjct: 421 IEKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKKCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQID 525
           VFE DVYEFLG ENAINKFCSYGSTGAACVATQ+EYWVNKLQI+
Sbjct: 481 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIN 522

BLAST of MS008352 vs. ExPASy TrEMBL
Match: A0A1S3CQK7 (argininosuccinate lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503657 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.3e-277
Identity = 485/524 (92.56%), Postives = 500/524 (95.42%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           M+ L SYSLP   AFAFSKPSVS SKPPFLA +R AMP+FGVL AS TA+A PSV DSP+
Sbjct: 1   MDCLTSYSLP--AAFAFSKPSVSLSKPPFLAVARGAMPMFGVLRASHTANAPPSVYDSPE 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           N  DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLAKQGLMSVDE
Sbjct: 61  NRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSILEGLD+IER+IESG+FVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAID ILDAIKYLQVSMVTLAL NEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDAILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLNF PLGACALAGTGLPIDRFMTA+ALGFT PLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFTGPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFI PNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNIS+NRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           I K+LPAG+LDATTLADYLVKKGIPFRTGHDIVGK VALCVTK CCLQDLSLDELRSISP
Sbjct: 421 IEKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKKCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQID 525
           VFE DVYEFLG ENAINKFCSYGSTGAACVATQ+EYWVNKLQI+
Sbjct: 481 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIN 522

BLAST of MS008352 vs. ExPASy TrEMBL
Match: A0A6J1IGY4 (argininosuccinate lyase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477131 PE=3 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.4e-273
Identity = 480/524 (91.60%), Postives = 495/524 (94.47%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           M+ L SY LP     AFSK SVSASK PF A +R A+PVFGVL ASRTADA  SV D P+
Sbjct: 1   MDCLTSYFLP--ATLAFSKSSVSASKLPFFAVARGALPVFGVLRASRTADAPLSVDDWPE 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           N +DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLA QGLMSV E
Sbjct: 61  NRSDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLANQGLMSVAE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSI+EGLDEIERRIESG+FVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSIIEGLDEIERRIESGDFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAID ILDAI+YLQVSMVTLAL NEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDAILDAIRYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLN CPLGACALAGTGLPIDRFMTA+ALGFTAPLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNLCPLGACALAGTGLPIDRFMTAEALGFTAPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFI PNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNIS+NRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISFNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           I K+LPAG+LDATTLADYLVKKGIPFRTGHDIVGK VALCVTK+CCLQDLSLDELRSISP
Sbjct: 421 IEKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKSCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQID 525
           VFE DVYEFLG ENAINKFCSYGSTGAACVATQ+EYWVNKLQI+
Sbjct: 481 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQIEYWVNKLQIN 522

BLAST of MS008352 vs. ExPASy TrEMBL
Match: A0A0A0LE21 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G891650 PE=3 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.3e-272
Identity = 476/524 (90.84%), Postives = 494/524 (94.27%), Query Frame = 0

Query: 1   MEALGSYSLPTATAFAFSKPSVSASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPD 60
           M+ L SYSLP   AF FS PSVS SKPPF A +R AM + GVL AS TA+A PSV DSP+
Sbjct: 1   MDCLTSYSLP--AAFTFSNPSVSLSKPPFFAVARGAMSMVGVLRASHTANAPPSVDDSPE 60

Query: 61  NHADKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDE 120
           N  DKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHA+MLAKQGLMSVDE
Sbjct: 61  NRTDKEVKLWGGRFEESVTDAVERFTESISYDKQLYKHDIRGSRAHATMLAKQGLMSVDE 120

Query: 121 RDSILEGLDEIERRIESGEFVWRTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLT 180
           RDSILEGLD+IER+IESG+FVWRTDREDVHMNIEAALID+IGEPAKKLHTARSRNDQVLT
Sbjct: 121 RDSILEGLDQIERQIESGDFVWRTDREDVHMNIEAALIDIIGEPAKKLHTARSRNDQVLT 180

Query: 181 DFRLWCRDAIDKILDAIKYLQVSMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240
           DFRLWCRDAID ILDAIKYLQVSMVTLAL NEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ
Sbjct: 181 DFRLWCRDAIDSILDAIKYLQVSMVTLALKNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQ 240

Query: 241 LERDAGRLSDCRVRLNFCPLGACALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFA 300
           LERDAGRLSDCRVRLNF PLGACALAGTGLPIDRFMTA+ALGF  PLRNSIDAVSDRDFA
Sbjct: 241 LERDAGRLSDCRVRLNFSPLGACALAGTGLPIDRFMTAEALGFAGPLRNSIDAVSDRDFA 300

Query: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGK 360
           LEFLSANAITAIHLSRLGEEWVLWASEEFGFI PNDSVSTGSSIMPQKKNPDPMELVRGK
Sbjct: 301 LEFLSANAITAIHLSRLGEEWVLWASEEFGFITPNDSVSTGSSIMPQKKNPDPMELVRGK 360

Query: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRER 420
           SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTI+GMLEVSAEFAQNIS+NRER
Sbjct: 361 SARVIGDLVTTLTLCKGLPLAYNRDLQEDKEPVFDSVKTIVGMLEVSAEFAQNISFNRER 420

Query: 421 IRKSLPAGFLDATTLADYLVKKGIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISP 480
           I K+LPAG+LDATTLADYLVKKGIPFRTGHDIVGK VALCVTK CCLQDLSLDELRSISP
Sbjct: 421 ISKALPAGYLDATTLADYLVKKGIPFRTGHDIVGKSVALCVTKRCCLQDLSLDELRSISP 480

Query: 481 VFEADVYEFLGPENAINKFCSYGSTGAACVATQLEYWVNKLQID 525
           VFE DVYEFLG ENAINKFCSYGSTGAACVATQ++YWVNKLQI+
Sbjct: 481 VFEEDVYEFLGAENAINKFCSYGSTGAACVATQIDYWVNKLQIN 522

BLAST of MS008352 vs. TAIR 10
Match: AT5G10920.1 (L-Aspartase-like family protein )

HSP 1 Score: 748.8 bits (1932), Expect = 2.9e-216
Identity = 372/501 (74.25%), Postives = 425/501 (84.83%), Query Frame = 0

Query: 23  SASKPPFLAFSRRAMPVFGVLSASRTADAAPSVADSPDNHADKEVKLWGGRFEESVTDAV 82
           S+S+    + + R++      S SR      S++   D+ A KEVKLWGGRFEESVT+ V
Sbjct: 15  SSSRSNLSSSTHRSVSFLPPGSKSRCLPPLRSMSHD-DDTASKEVKLWGGRFEESVTEKV 74

Query: 83  ERFTESISYDKQLYKHDIRGSRAHASMLAKQGLMSVDERDSILEGLDEIERRIESGEFVW 142
           E+FTESIS+DK LYK DI GS+AHASMLA QGL++  ++DSIL GLD+IER+IE+ +F W
Sbjct: 75  EKFTESISFDKVLYKQDIMGSKAHASMLAHQGLITDSDKDSILRGLDDIERQIEANKFEW 134

Query: 143 RTDREDVHMNIEAALIDMIGEPAKKLHTARSRNDQVLTDFRLWCRDAIDKILDAIKYLQV 202
           RTDREDVHMNIEAAL D+IGEPAKKLHTARSRNDQV TDFRLWCRDAID I+  I+ LQ 
Sbjct: 135 RTDREDVHMNIEAALTDLIGEPAKKLHTARSRNDQVATDFRLWCRDAIDTIIVKIRNLQR 194

Query: 203 SMVTLALSNEGLIVPGYTHLQRAQPVLLQHLLLAFVEQLERDAGRLSDCRVRLNFCPLGA 262
           ++V LAL NE LIVPGYTHLQRAQPVLL H+LL FVEQLERDAGR  DCR RLNF PLGA
Sbjct: 195 ALVELALKNEALIVPGYTHLQRAQPVLLPHVLLTFVEQLERDAGRYVDCRARLNFSPLGA 254

Query: 263 CALAGTGLPIDRFMTADALGFTAPLRNSIDAVSDRDFALEFLSANAITAIHLSRLGEEWV 322
           CALAGTGLPIDRFMTA+ALGFT P+RNSIDAVSDRDF LEFL  NA T IHLSRLGEEWV
Sbjct: 255 CALAGTGLPIDRFMTANALGFTEPMRNSIDAVSDRDFVLEFLYTNANTGIHLSRLGEEWV 314

Query: 323 LWASEEFGFIMPNDSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTTLTLCKGLPLAY 382
           LWASEEFGF+ P+DSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVT LTLCKGLPLAY
Sbjct: 315 LWASEEFGFMTPSDSVSTGSSIMPQKKNPDPMELVRGKSARVIGDLVTVLTLCKGLPLAY 374

Query: 383 NRDLQEDKEPVFDSVKTILGMLEVSAEFAQNISYNRERIRKSLPAGFLDATTLADYLVKK 442
           NRD QEDKEP+FDS KTI+GM++VSAEFAQN+++N +RI+KSLPAG LDATTLADYLVKK
Sbjct: 375 NRDFQEDKEPMFDSTKTIMGMIDVSAEFAQNVTFNEDRIKKSLPAGHLDATTLADYLVKK 434

Query: 443 GIPFRTGHDIVGKCVALCVTKNCCLQDLSLDELRSISPVFEADVYEFLGPENAINKFCSY 502
           G+PFR+ HDIVGK V +CV+K C LQ+LSL+E++ +SPVFE DV+ FLG EN++NKF SY
Sbjct: 435 GMPFRSSHDIVGKLVGVCVSKGCELQNLSLEEMKKLSPVFEEDVFGFLGVENSVNKFSSY 494

Query: 503 GSTGAACVATQLEYWVNKLQI 524
           GSTG+ CVA QL YWVNKL I
Sbjct: 495 GSTGSNCVAEQLGYWVNKLNI 514

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142042.13.1e-30099.24argininosuccinate lyase, chloroplastic-like [Momordica charantia][more]
XP_008466162.12.8e-27792.56PREDICTED: argininosuccinate lyase, chloroplastic [Cucumis melo] >KAA0038666.1 a... [more]
XP_038900244.12.4e-27691.98argininosuccinate lyase, chloroplastic-like [Benincasa hispida][more]
XP_023536382.13.2e-27391.79argininosuccinate lyase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022976892.17.1e-27391.60argininosuccinate lyase, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LEU84.1e-21574.25Argininosuccinate lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10... [more]
Q6AR608.2e-13150.67Argininosuccinate lyase OS=Desulfotalea psychrophila (strain LSv54 / DSM 12343) ... [more]
B8GSV21.4e-13053.24Argininosuccinate lyase OS=Thioalkalivibrio sulfidiphilus (strain HL-EbGR7) OX=3... [more]
Q67S727.7e-12951.68Argininosuccinate lyase OS=Symbiobacterium thermophilum (strain T / IAM 14863) O... [more]
Q2SQ676.5e-12853.39Argininosuccinate lyase OS=Hahella chejuensis (strain KCTC 2396) OX=349521 GN=ar... [more]
Match NameE-valueIdentityDescription
A0A6J1CLI11.5e-30099.24argininosuccinate lyase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LO... [more]
A0A5A7T8Y21.3e-27792.56Argininosuccinate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CQK71.3e-27792.56argininosuccinate lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503657 P... [more]
A0A6J1IGY43.4e-27391.60argininosuccinate lyase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A0A0LE211.3e-27290.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G891650 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G10920.12.9e-21674.25L-Aspartase-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00145ARGSUCLYASEcoord: 167..189
score: 68.79
coord: 297..321
score: 52.07
coord: 259..275
score: 67.02
coord: 341..357
score: 81.13
coord: 376..395
score: 76.96
coord: 208..228
score: 57.99
NoneNo IPR availableGENE3D1.20.200.10Fumarase/aspartase (Central domain)coord: 185..514
e-value: 1.0E-181
score: 606.5
NoneNo IPR availableGENE3D1.10.40.30coord: 426..498
e-value: 1.0E-181
score: 606.5
NoneNo IPR availablePANTHERPTHR43814:SF4ARGININOSUCCINATE LYASEcoord: 79..524
IPR000362Fumarate lyase familyPRINTSPR00149FUMRATELYASEcoord: 341..357
score: 64.09
coord: 168..186
score: 36.91
coord: 297..324
score: 27.87
coord: 213..231
score: 41.79
IPR022761Fumarate lyase, N-terminalPFAMPF00206Lyase_1coord: 72..366
e-value: 3.6E-67
score: 227.0
IPR029419Argininosuccinate lyase, C-terminalPFAMPF14698ASL_C2coord: 429..496
e-value: 3.9E-24
score: 85.2
IPR024083Fumarase/histidase, N-terminalGENE3D1.10.275.10coord: 88..295
e-value: 1.0E-181
score: 606.5
IPR009049Argininosuccinate lyaseTIGRFAMTIGR00838TIGR00838coord: 69..516
e-value: 9.6E-171
score: 566.7
IPR009049Argininosuccinate lyasePANTHERPTHR43814ARGININOSUCCINATE LYASEcoord: 79..524
IPR009049Argininosuccinate lyaseHAMAPMF_00006Arg_succ_lyasecoord: 68..519
score: 40.079575
IPR009049Argininosuccinate lyaseCDDcd01359Argininosuccinate_lyasecoord: 88..521
e-value: 0.0
score: 649.223
IPR020557Fumarate lyase, conserved sitePROSITEPS00163FUMARATE_LYASEScoord: 341..350
IPR008948L-Aspartase-likeSUPERFAMILY48557L-aspartase-likecoord: 69..521

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008352.1MS008352.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042450 arginine biosynthetic process via ornithine
molecular_function GO:0004056 argininosuccinate lyase activity
molecular_function GO:0003824 catalytic activity