MS008337 (gene) Bitter gourd (TR) v1

Overview
NameMS008337
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRhamnogalacturonan endolyase
Locationscaffold4: 634993 .. 639875 (+)
RNA-Seq ExpressionMS008337
SyntenyMS008337
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AGACAAGACGATGTTCTTTGCATGTGTGGGGAGCTGCAGGTAGTTATGGACAATGGTATATTACAAGTGACACTGTCCAAGCCAGATGGAAATGTTACTGGGATCAAATACAACAGCATTGATAATGTTTTAGAAGTTGATGATAAGGAAAATCATAGAGGGTATATGATTTCAATTATTAATATCAAGCCTCTCTGTCTTTCTCCAATTATGATCATCTAGTTAATTCCAAAATTTAAGTTTACTCAGATTGATTCAGTATATGATGCAGGTACTGGGACCTTGTGTGGAACTCGGGGACTGGAGGAACAACGGGAACGTTCGATGAGTATGCAAGAAAAAAATAGTTATTAAGGCCTTGTTTGATAACCATTTCGTTTTTCATTTTCTGTTTTTGAAAATTATGTTTGTTCACTCACAATTTTCTAAGCATATTTTCACCTTTCTTGAAAATATATTTGCATTCTTAGCCAAATTTAAAAAACAAGAACAAGTTTTTGAAAATTACTTTTTTTAGTTTTCAAAATTTGACTTAGATTTTGAAAACGTTTTAAAAAAATAGGTAGCAAAACAAAGAAATACATGGGTAAAAAGTAGTGTTTACCACCTTAATTTTCAAAATCAGAAAACCAAAAACAAAATTATTATCAAACGGAGCATTCAATTTATTCATTTTTGTAGTATTACAGTTGGATGGGGTCTTTCTGATTCTACCTAAAGAAACTTTTCTGTTGAAATGGCATCAAGATATTTATAATGCTTTCAGGATGAAAGGAAAAGAATTTGAAGTGATAGTGCAAAATGAGGAACAGATAGAGCTCTCATTTAAAAGGAATTATTCATTCAAGGACAAGGCCGTTCCCTTAAACATTGATAAAAGGTGCCAGCAATCTGCAGCTGCATCATGAATCTAATAAGCTTTTGTTCTTATGTCAAGTGTATGATCAATATGAACTTGAAAAAGAAAAATGAAACAAACAAAAACTGATAGAAGTTCCTCTTTCAGGTTCATAATGCTTCGTAATTCCTCTGGATTTTATTCCTATGCCATCTATGAGCACTTGAAGGAATGGCCTCCATTCAACCTTCCCCAAACTAGGATTGTGTTCAAGCTAAGAAAAGACAAGTAAAAAGACCTATATTTCTACTTCCTTTTTAATATATGTCTGTTGCTGAGAATCATTAATTAATGGTGAGTTTTTTCTTTAATTGTAATTTAGGTTTCATTATATGGCAATAGCAGATAACAGACAGAGATTCATGCCACTTCCTGAGGATAGACTGCGTCACAGAGGCAAAACCTTGGACTTTCCAGAGGCTGTAAAGCTAGTCAATCCTAAAGAACCTCAGTTCAAGGGAGAGGTTCTTATACTTCTTCATGATTTTCCATTGTCTGGACTCGCATTTTTCTGTTCTTTATTGTGAAATCAGTTACTTTAAGGTCTCGTTTAATAACTATTTTGTTTTTAATTTTCGGTTTTTAAAAATTAAGCTGGTAAACTCTACTTTAATCCATGTATTTTTTTATTTTGCTACCTACTTTGTAAAAATAATTTCGAAATCTAGAAAACCAAATACAATAGGTTGAGTTCAAAGGTATGCATATTTGGGAGTTTTCATCTTCCTTATATATGTGAAAATAAAACGCTATCGGATTAGGATAACACTAAATCGACGAAAAATGTCCATACTGAAACAAACAGCTTTGTAGGCCAAGCTGAAACCTTTTGCTGCTAAATTTGTAAGAATCTGATATCAGTGGAACATGTGGATGTGAGTAGGTGGATGATAAGTACCAATATTCTTGTGAAAATAAAGATCTTAAAGTGCATGGATGGATATCCATGGATCCTGCAGTGGGTTTCTGGCAAATCACTCCCAGCAGTGAGTTCAGATCAGGTGGACCTCTCAAACAGAACCTTACTTCTCATGTGGGACCATATGCTCTTGCTGTAAGTATATATAATTTCTGGTATATGAGCAAGTTGAAGTTAATTGAAGATATAAGTTGAAGTTAATTGAAGATATTAGTGCATATTATTATTAATTGAACCAAAATGCCACAATTTCAGATGTTCCTCAGCGCTCACTATTCAGGAGAGGACTTGGTGCTGAAACTCAATCCTGATGAGCCGTGGAAGAAAGTTTTTGGCCCTGTATTTCTATATCTAAATTCTGTTACTGATGGAGGTGACCCACTTTCTTTGTGGAAAGATGCTAAAACTCAGGTTTGTTTATCATAACTCTTAGTGCCTGTCATTCGTGTTAAATCACTCATCAATCAAAAAACTTAAGTTGATGAATTATGGCAAATTCAATATTTTATAGATACTTTAATACTCCTTACTTGTGGGCTTGAAAATTTGCACAAGACCCAACAAGTGACAATCGCTATTAAATGGGGAGGAAATGACATTACAAGGTTCAAACACAAGATATTCTGCTTTGATACCATGTTAAATCACCAATCAACCAAAGAAGTTTAAGTTGATGAGTTACGATAAATTTAATCTTTTATAAATACCTTAACAATTTCTCGTAATGGTAGAAGTTGGATCTTGTATTTTAATTCAGATCCTGAAAATGGTCGTTTCTTTCACAACTATGCCTATGAATTTATCTTGTGCATTACTATTTCCCAGATGACGAAGGAAGTTCAAAGCTGGCCATATTCTTTTCCAGCTTCTGAGGATTTTCCCCCACCAAACCAACGTGGTAGATTGACTGGAAAATTGTTTGTCCTTGACAGGTATCATTCATAAAAACACCCACACAAAAGTTGACCCACCATCAAAAGTATTCATAGCCAGCCTTTTCTGTTCATTAGTAAAAATGCCAAACTCTAACCAAACTATTATTTGATCCGAATGGAAAGTAGTTAATTGTTACGGCTCTTAAAACATGTTTTAGATTGGTGAAGTTTTGAACTGAACAATTTTCAATTCATTGCACTTGTATTTAGATACGTTAGCCAAGATCAAACGCCAGCAACCGACGCTTATGTGGGACTGGCAGCACCAGGAGAAGCTGGATCATGGCAGACAGAAAACAAGGTACTGATCATTAATTTTCAACAACATACTAAATAATGAATGCTGAATGCAAAGAAAATGAGATGTTATTTATTTAAACCATGATCAGGGTTACCAGTTTTGGACCAAAACAGACGCAGATGGCTCGTTTAGCATCGAAAATGTACGCATCGGCGACTACAACCTTTATGCTTGGGTTCCCGGTTTCATTGGCGATTATCGTTACGACGTCATCGTTAGCATCTCCGCAGGTCTTGGTTCACTTTCTCCAAATGAAGCTCATAATTCAAGAACTGAATTGATTTTCAGCAAGCATTTGTGACTCTATTCATGTTCGATCTTTCAGGTAGTGATATGGACGTGGGTGATCTTGTGTATGAGCCGCCCAGGAACGGGCCTACATTGTGGGAAATAGGCATTCCTGATCGATCTGCTGCTGAATTCTATGTTCCTGATCCTGATCCAAATTATATCAACAAATTGTACGTCGATCATCCTGATAGGTAAGAAAAATGTTCACAATTAATAATCATATGACATCCAATATGTTAATATATATAACTGTGATGGTAGAAGTATAATCCTCAGAATAATATGTTCCTTTTTTTTTCCCTTTACATCTTCCCAAAGGTTGTGATCGAAGTAAAATATTTTAAAAGAATACGATGGATAAATAATAACGTGACCTTCATAACTTTGTATTTATGCTGAGCAGATTTAGGCAATATGGATTGTGGGAAAGATATGCAGAATTGTATCCTGATGCAGACTTGGTATATACGGTTGGTGTTAGTGACTACACAAAAGATTGGTTTTTTGCTCATGTTAATAGGTAAGTATGTGTAGCCTATTATTTCTGTAATGAATTTAAATGTATCATGCTTATAATTCTTCTGTTATTTCTTTCTTCTTTTACCTTTTTAAATGACAGAACGAGAAATGATGGTTCCCATCTTAGCACCACGTGGCAAATTAAGTTTACAGTTGATATCGTAGATAAGAATGGCGCCTATACGTTGCGAGTGGCTCTCGCTACTGCCAATGTTGCTGAATTACAGGTACCCATCCTATTTATGAGACATTTGCATATATAATACATTTAAGAATTAAAATTGGAAAAGTAGCCTAAAAAAATTCAACAATAAATTTTTTATAACATATAACTATTAGCAGTAGTTAAAACATAGCTTAGCTAATTAAAATATTATTATCATTGGAGGTTTGAATCCTCGTCCTATGGATTCTTCGTGAACTCAATAATAAAATAAATAAAATTAAAAATACATTTCATCTATTATCAATAATTTCTTTTAGTACAACAAGGGGGTGATGACTCGAACTCATGACTTATTTGTCATAGACATTGTCAATTATCGTTGAACCATTTAAAATCATGGATTCATTTGATTTATCAAATTGGTCACTTTCCGTCCACCTGGGGTGTTTTTCTTGTCTACAAAACTGAACATTTCAAAATAAGGTGTAATTAGAAAATAATAATAATATTATATCACCACGGGTGAAAAGTAAAATCTCAAACATGTTTTGACTATACATTATTTATATATATTAAAAAAACGTGATTTCTTTTCTTTTATTTTTTAAAAGATTCAGGTAAACGATCCAGAAGCAAAGCCAGCTGTATTCACGACAGGACAGATTGGGCACGACAATACAATAGCTAGGCATGGAATTCATGGACTTTATCGACTGTACAGTGTCGACATGTCGGGATCTCTGCTTGTAGAAGGAGAAAACACCATCTTCCTCTCGCAAATTATAAGCAGTTCTTCTTTCAATGGTGTTATGTATGATTATATCCGCCTCGAAGCCCCCCCATCTGATAACCCC

mRNA sequence

AGACAAGACGATGTTCTTTGCATGTGTGGGGAGCTGCAGGTAGTTATGGACAATGGTATATTACAAGTGACACTGTCCAAGCCAGATGGAAATGTTACTGGGATCAAATACAACAGCATTGATAATGTTTTAGAAGTTGATGATAAGGAAAATCATAGAGGGTACTGGGACCTTGTGTGGAACTCGGGGACTGGAGGAACAACGGGAACGTTCGATGAGATGAAAGGAAAAGAATTTGAAGTGATAGTGCAAAATGAGGAACAGATAGAGCTCTCATTTAAAAGGAATTATTCATTCAAGGACAAGGCCGTTCCCTTAAACATTGATAAAAGGTTCATAATGCTTCGTAATTCCTCTGGATTTTATTCCTATGCCATCTATGAGCACTTGAAGGAATGGCCTCCATTCAACCTTCCCCAAACTAGGATTGTGTTCAAGCTAAGAAAAGACAAGTTTCATTATATGGCAATAGCAGATAACAGACAGAGATTCATGCCACTTCCTGAGGATAGACTGCGTCACAGAGGCAAAACCTTGGACTTTCCAGAGGCTGTAAAGCTAGTCAATCCTAAAGAACCTCAGTTCAAGGGAGAGGTGGATGATAAGTACCAATATTCTTGTGAAAATAAAGATCTTAAAGTGCATGGATGGATATCCATGGATCCTGCAGTGGGTTTCTGGCAAATCACTCCCAGCAGTGAGTTCAGATCAGGTGGACCTCTCAAACAGAACCTTACTTCTCATGTGGGACCATATGCTCTTGCTATGTTCCTCAGCGCTCACTATTCAGGAGAGGACTTGGTGCTGAAACTCAATCCTGATGAGCCGTGGAAGAAAGTTTTTGGCCCTGTATTTCTATATCTAAATTCTGTTACTGATGGAGGTGACCCACTTTCTTTGTGGAAAGATGCTAAAACTCAGATGACGAAGGAAGTTCAAAGCTGGCCATATTCTTTTCCAGCTTCTGAGGATTTTCCCCCACCAAACCAACGTGGTAGATTGACTGGAAAATTGTTTGTCCTTGACAGATACGTTAGCCAAGATCAAACGCCAGCAACCGACGCTTATGTGGGACTGGCAGCACCAGGAGAAGCTGGATCATGGCAGACAGAAAACAAGGGTTACCAGTTTTGGACCAAAACAGACGCAGATGGCTCGTTTAGCATCGAAAATGTACGCATCGGCGACTACAACCTTTATGCTTGGGTTCCCGGTTTCATTGGCGATTATCGTTACGACGTCATCGTTAGCATCTCCGCAGGTAGTGATATGGACGTGGGTGATCTTGTGTATGAGCCGCCCAGGAACGGGCCTACATTGTGGGAAATAGGCATTCCTGATCGATCTGCTGCTGAATTCTATGTTCCTGATCCTGATCCAAATTATATCAACAAATTGTACGTCGATCATCCTGATAGCAGATTTAGGCAATATGGATTGTGGGAAAGATATGCAGAATTGTATCCTGATGCAGACTTGGTATATACGGTTGGTGTTAGTGACTACACAAAAGATTGGTTTTTTGCTCATGTTAATAGAACGAGAAATGATGGTTCCCATCTTAGCACCACGTGGCAAATTAAGTTTACAGTTGATATCGTAGATAAGAATGGCGCCTATACGTTGCGAGTGGCTCTCGCTACTGCCAATGTTGCTGAATTACAGGTAAACGATCCAGAAGCAAAGCCAGCTGTATTCACGACAGGACAGATTGGGCACGACAATACAATAGCTAGGCATGGAATTCATGGACTTTATCGACTGTACAGTGTCGACATGTCGGGATCTCTGCTTGTAGAAGGAGAAAACACCATCTTCCTCTCGCAAATTATAAGCAGTTCTTCTTTCAATGGTGTTATGTATGATTATATCCGCCTCGAAGCCCCCCCATCTGATAACCCC

Coding sequence (CDS)

AGACAAGACGATGTTCTTTGCATGTGTGGGGAGCTGCAGGTAGTTATGGACAATGGTATATTACAAGTGACACTGTCCAAGCCAGATGGAAATGTTACTGGGATCAAATACAACAGCATTGATAATGTTTTAGAAGTTGATGATAAGGAAAATCATAGAGGGTACTGGGACCTTGTGTGGAACTCGGGGACTGGAGGAACAACGGGAACGTTCGATGAGATGAAAGGAAAAGAATTTGAAGTGATAGTGCAAAATGAGGAACAGATAGAGCTCTCATTTAAAAGGAATTATTCATTCAAGGACAAGGCCGTTCCCTTAAACATTGATAAAAGGTTCATAATGCTTCGTAATTCCTCTGGATTTTATTCCTATGCCATCTATGAGCACTTGAAGGAATGGCCTCCATTCAACCTTCCCCAAACTAGGATTGTGTTCAAGCTAAGAAAAGACAAGTTTCATTATATGGCAATAGCAGATAACAGACAGAGATTCATGCCACTTCCTGAGGATAGACTGCGTCACAGAGGCAAAACCTTGGACTTTCCAGAGGCTGTAAAGCTAGTCAATCCTAAAGAACCTCAGTTCAAGGGAGAGGTGGATGATAAGTACCAATATTCTTGTGAAAATAAAGATCTTAAAGTGCATGGATGGATATCCATGGATCCTGCAGTGGGTTTCTGGCAAATCACTCCCAGCAGTGAGTTCAGATCAGGTGGACCTCTCAAACAGAACCTTACTTCTCATGTGGGACCATATGCTCTTGCTATGTTCCTCAGCGCTCACTATTCAGGAGAGGACTTGGTGCTGAAACTCAATCCTGATGAGCCGTGGAAGAAAGTTTTTGGCCCTGTATTTCTATATCTAAATTCTGTTACTGATGGAGGTGACCCACTTTCTTTGTGGAAAGATGCTAAAACTCAGATGACGAAGGAAGTTCAAAGCTGGCCATATTCTTTTCCAGCTTCTGAGGATTTTCCCCCACCAAACCAACGTGGTAGATTGACTGGAAAATTGTTTGTCCTTGACAGATACGTTAGCCAAGATCAAACGCCAGCAACCGACGCTTATGTGGGACTGGCAGCACCAGGAGAAGCTGGATCATGGCAGACAGAAAACAAGGGTTACCAGTTTTGGACCAAAACAGACGCAGATGGCTCGTTTAGCATCGAAAATGTACGCATCGGCGACTACAACCTTTATGCTTGGGTTCCCGGTTTCATTGGCGATTATCGTTACGACGTCATCGTTAGCATCTCCGCAGGTAGTGATATGGACGTGGGTGATCTTGTGTATGAGCCGCCCAGGAACGGGCCTACATTGTGGGAAATAGGCATTCCTGATCGATCTGCTGCTGAATTCTATGTTCCTGATCCTGATCCAAATTATATCAACAAATTGTACGTCGATCATCCTGATAGCAGATTTAGGCAATATGGATTGTGGGAAAGATATGCAGAATTGTATCCTGATGCAGACTTGGTATATACGGTTGGTGTTAGTGACTACACAAAAGATTGGTTTTTTGCTCATGTTAATAGAACGAGAAATGATGGTTCCCATCTTAGCACCACGTGGCAAATTAAGTTTACAGTTGATATCGTAGATAAGAATGGCGCCTATACGTTGCGAGTGGCTCTCGCTACTGCCAATGTTGCTGAATTACAGGTAAACGATCCAGAAGCAAAGCCAGCTGTATTCACGACAGGACAGATTGGGCACGACAATACAATAGCTAGGCATGGAATTCATGGACTTTATCGACTGTACAGTGTCGACATGTCGGGATCTCTGCTTGTAGAAGGAGAAAACACCATCTTCCTCTCGCAAATTATAAGCAGTTCTTCTTTCAATGGTGTTATGTATGATTATATCCGCCTCGAAGCCCCCCCATCTGATAACCCC

Protein sequence

RQDDVLCMCGELQVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALATANVAELQVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQIISSSSFNGVMYDYIRLEAPPSDNP
Homology
BLAST of MS008337 vs. NCBI nr
Match: XP_022136955.1 (probable rhamnogalacturonate lyase B [Momordica charantia])

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 617/623 (99.04%), Postives = 617/623 (99.04%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE
Sbjct: 15  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 74

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW
Sbjct: 75  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 134

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP
Sbjct: 135 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 194

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 253
           QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA
Sbjct: 195 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 254

Query: 254 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 313
           LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ
Sbjct: 255 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 314

Query: 314 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 373
           SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK
Sbjct: 315 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 374

Query: 374 GYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 433
           GYQFWTKTDADGSFSIEN RIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP
Sbjct: 375 GYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 434

Query: 434 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADL 493
           PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPD RFRQYGLWERYAELYPDADL
Sbjct: 435 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPD-RFRQYGLWERYAELYPDADL 494

Query: 494 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALATANVAE 553
           VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD VDKN AYTLRVALATANVAE
Sbjct: 495 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAYTLRVALATANVAE 554

Query: 554 L--QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQII 613
           L  QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQII
Sbjct: 555 LQIQVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQII 614

Query: 614 SSSSFNGVMYDYIRLEAPPSDNP 635
           SSSSFNGVMYDYIRLEAPPSDNP
Sbjct: 615 SSSSFNGVMYDYIRLEAPPSDNP 636

BLAST of MS008337 vs. NCBI nr
Match: XP_008466141.1 (PREDICTED: probable rhamnogalacturonate lyase B [Cucumis melo])

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 569/623 (91.33%), Postives = 602/623 (96.63%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLVWNSGT GTTGTFDE
Sbjct: 15  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKETHRGYWDLVWNSGTSGTTGTFDE 74

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           M+GKEFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEH++EW
Sbjct: 75  MRGKEFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHMREW 134

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK LDFPEAV LVNPKEP
Sbjct: 135 PPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEP 194

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 253
           +FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGGPLKQNLTSHVGPYA
Sbjct: 195 EFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 254

Query: 254 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 313
           LAMFLSAHYSGEDLVLKL+PDEPWKKVFGPVF YLNSV+DGGDPLSLW+DAKTQM+ EVQ
Sbjct: 255 LAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQMSIEVQ 314

Query: 314 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 373
           SWPYSFPASEDFPPPNQRGRLTGKLFVLDRY+++++ PATDAYVGLAAPGEAGSWQTENK
Sbjct: 315 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYINEEKMPATDAYVGLAAPGEAGSWQTENK 374

Query: 374 GYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 433
           GYQFWTKTDADGSFSIE+VRIGDYNLYAWVPGFIGDYRYDV++++S GSDMDVGDLVYEP
Sbjct: 375 GYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVSPGSDMDVGDLVYEP 434

Query: 434 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADL 493
           PRNGPTLWEIGIPDRSAAEFY+PDPDP YINKLYV+HPD RFRQYGLWERYAELYPDADL
Sbjct: 435 PRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYVNHPD-RFRQYGLWERYAELYPDADL 494

Query: 494 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD-IVDKNGAYTLRVALATANVA 553
           VY+VGVSDYTKDWFFAHVNR RNDGSHL+TTW+IKF VD I+DK+G YTLRVALATANVA
Sbjct: 495 VYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRVDNIIDKSGTYTLRVALATANVA 554

Query: 554 EL--QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQI 613
           EL  QVN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSVD+ GSLLVEGENTIFLSQI
Sbjct: 555 ELQIQVNNPEAKPSLFTTGMIGHDNAIARHGIHGLYRLYSVDILGSLLVEGENTIFLSQI 614

Query: 614 ISSSSFNGVMYDYIRLEAPPSDN 634
           I+SSSFNGVMYDYIRLE PPSD+
Sbjct: 615 INSSSFNGVMYDYIRLEGPPSDS 636

BLAST of MS008337 vs. NCBI nr
Match: XP_038899392.1 (probable rhamnogalacturonate lyase B [Benincasa hispida])

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 569/622 (91.48%), Postives = 602/622 (96.78%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLVWNSGT GTTGTFDE
Sbjct: 15  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLVWNSGTPGTTGTFDE 74

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           M+GKEFEVIVQNEEQIELSFKRNY FKD+AVPLNIDKRFIMLRNSSGFYSYAIYEH+KEW
Sbjct: 75  MRGKEFEVIVQNEEQIELSFKRNYPFKDEAVPLNIDKRFIMLRNSSGFYSYAIYEHMKEW 134

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIAD+R+RFMPLPEDRLRHRGK LDFPEAV LVNPKEP
Sbjct: 135 PPFNLPQTRIVFKLRKDKFHYMAIADDRRRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEP 194

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 253
           +FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGGPLKQNLTSHVGPYA
Sbjct: 195 KFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 254

Query: 254 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 313
           LAMFLSAHYSGEDLVLKL+PDEPWKKVFGPVF YLNSV+DG DPLSLW+DAKTQMTKEVQ
Sbjct: 255 LAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGVDPLSLWEDAKTQMTKEVQ 314

Query: 314 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 373
           SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYV+QD+  ATDAYVGLAAPG+AGSWQTENK
Sbjct: 315 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNQDKMLATDAYVGLAAPGDAGSWQTENK 374

Query: 374 GYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 433
           GYQFWTKTDADGSFSIE++RIGDYNLYAWVPGFIGDYRYD ++S+SAGSDMDVGDLVYEP
Sbjct: 375 GYQFWTKTDADGSFSIEHIRIGDYNLYAWVPGFIGDYRYDAVISVSAGSDMDVGDLVYEP 434

Query: 434 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADL 493
           PRNGPTLWEIGIPDRSAAEFYVPDPDP YINKLYV+HPD RFRQYGLWE+YAELYPDADL
Sbjct: 435 PRNGPTLWEIGIPDRSAAEFYVPDPDPKYINKLYVNHPD-RFRQYGLWEKYAELYPDADL 494

Query: 494 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD-IVDKNGAYTLRVALATANVA 553
           VYTVGVSDYTKDWFFAHVNRTRNDGSHL+TTW+IKFT+D +++K+G YTLRVALATANVA
Sbjct: 495 VYTVGVSDYTKDWFFAHVNRTRNDGSHLATTWEIKFTLDNVIEKSGTYTLRVALATANVA 554

Query: 554 EL--QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQI 613
           EL  QVN+PEAKP++FTTG IGHDNTIARHGIHGLYRLYSVD+ GSLLVEG+NTIFLSQI
Sbjct: 555 ELQIQVNNPEAKPSLFTTGVIGHDNTIARHGIHGLYRLYSVDILGSLLVEGDNTIFLSQI 614

Query: 614 ISSSSFNGVMYDYIRLEAPPSD 633
           I++SSFNGVMYDYIRLE PPSD
Sbjct: 615 INNSSFNGVMYDYIRLEGPPSD 635

BLAST of MS008337 vs. NCBI nr
Match: XP_004136251.1 (probable rhamnogalacturonate lyase B [Cucumis sativus] >KGN60274.1 hypothetical protein Csa_002470 [Cucumis sativus])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 559/622 (89.87%), Postives = 600/622 (96.46%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLVWNSGT GTTGTFDE
Sbjct: 15  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLVWNSGTPGTTGTFDE 74

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           M+G+EFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEH++EW
Sbjct: 75  MRGREFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHMREW 134

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK LDFPEAV LVNPKEP
Sbjct: 135 PPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEP 194

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 253
           +FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGGPLKQNLTSHVGPYA
Sbjct: 195 EFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 254

Query: 254 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 313
           LAMFLSAHYSGEDLVLK++PDEPWKKVFGPVF YLNSV+DGGDPLSLW+DAKTQM+KEVQ
Sbjct: 255 LAMFLSAHYSGEDLVLKVSPDEPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQMSKEVQ 314

Query: 314 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 373
           SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYV++++ PATDAYVGLAAPG+ GSWQTE K
Sbjct: 315 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNEEKMPATDAYVGLAAPGDVGSWQTEKK 374

Query: 374 GYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 433
           GYQFWTKTDADGSFSIE+VRIGDYNLYAWVPGFIGDYRYDV++++S+GSDMDVGDLVYEP
Sbjct: 375 GYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVSSGSDMDVGDLVYEP 434

Query: 434 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADL 493
           PRNGPTLWEIGIPDRSAAEFY+PDPDP YINKLY++HPD RFRQYGLWE+YAEL+PDADL
Sbjct: 435 PRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYINHPD-RFRQYGLWEKYAELHPDADL 494

Query: 494 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD-IVDKNGAYTLRVALATANVA 553
           VY+VGVSDYTKDWFFAHVNR RNDGSHL+TTW+IKF +D I+DK  +YTLRVALATANVA
Sbjct: 495 VYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRLDNIIDKGVSYTLRVALATANVA 554

Query: 554 EL--QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQI 613
           EL  QVN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSVD+ GSLLVEGENTIFLSQI
Sbjct: 555 ELQIQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVDILGSLLVEGENTIFLSQI 614

Query: 614 ISSSSFNGVMYDYIRLEAPPSD 633
           I++SSFNG+MYDYIRLE PPSD
Sbjct: 615 INNSSFNGIMYDYIRLEGPPSD 635

BLAST of MS008337 vs. NCBI nr
Match: XP_023535230.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1161.0 bits (3002), Expect = 0.0e+00
Identity = 552/626 (88.18%), Postives = 585/626 (93.45%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGIL VTLSKPDGNVTGIKY+SIDNVLEV DKE HRGYWDLVWNSGT GTTGTFDE
Sbjct: 16  VVMDNGILAVTLSKPDGNVTGIKYSSIDNVLEVRDKEAHRGYWDLVWNSGTPGTTGTFDE 75

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           M+GKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNSSGFYSYAIYEHL+EW
Sbjct: 76  MRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNSSGFYSYAIYEHLREW 135

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIADNR+R+MPLPEDR+  RGKTLDFPEAV LV+P+E 
Sbjct: 136 PPFNLPQTRIVFKLRKDKFHYMAIADNRKRWMPLPEDRVGRRGKTLDFPEAVLLVHPREA 195

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPA----VGFWQITPSSEFRSGGPLKQNLTSHV 253
            FKGEVDDKYQYSCENKDLKVHGWISMDP+    VGFWQITPSSEFRSGGPLKQNLTSHV
Sbjct: 196 AFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHV 255

Query: 254 GPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMT 313
           GPYALAMFLSAHY GE+LVLKLNPDEPWKKVFGPVF YLNSV +G DPLSLW+DAK QMT
Sbjct: 256 GPYALAMFLSAHYGGEELVLKLNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKIQMT 315

Query: 314 KEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQ 373
           KEVQSWPYSFPASE+FPPPNQRGRLTG LFVLDRY  Q+Q PA +AYVGLAAPG+AGSWQ
Sbjct: 316 KEVQSWPYSFPASENFPPPNQRGRLTGNLFVLDRYAKQEQMPAAEAYVGLAAPGDAGSWQ 375

Query: 374 TENKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDL 433
           TE KGYQFWTKTDA GSFSIENVRIGDYNLYAWVPGFIGDYRYDV++S+SAGSDMDVGD 
Sbjct: 376 TETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDF 435

Query: 434 VYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYP 493
           V+EPPRNGPTLWEIGIPDRSAAEFY+PDP+PNYINKLYVDHPD RFRQYGLWERYAELYP
Sbjct: 436 VFEPPRNGPTLWEIGIPDRSAAEFYIPDPNPNYINKLYVDHPD-RFRQYGLWERYAELYP 495

Query: 494 DADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD-IVDKNGAYTLRVALAT 553
           D DLVYTVGVSDYTKDWFFAHVNRTRNDGSHL TTWQIKFT+D  +DK+G Y LRVALAT
Sbjct: 496 DTDLVYTVGVSDYTKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALAT 555

Query: 554 ANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIF 613
           ANVAELQ  VN+PE + A+FTTGQIGHDN IARHGIHGLYRLYSVD+SGS+LVEGENTI 
Sbjct: 556 ANVAELQIRVNNPEVESALFTTGQIGHDNAIARHGIHGLYRLYSVDISGSVLVEGENTIL 615

Query: 614 LSQIISSSSFNGVMYDYIRLEAPPSD 633
           LSQII++SSFNGVMYDYIRLE PPSD
Sbjct: 616 LSQIINNSSFNGVMYDYIRLEGPPSD 640

BLAST of MS008337 vs. ExPASy Swiss-Prot
Match: Q8RJP2 (Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 3.0e-34
Identity = 164/629 (26.07%), Postives = 253/629 (40.22%), Query Frame = 0

Query: 16  MDNGILQVTLSKPDGNVTGIKYNSIDNVLEV-----DDKENHRGYWDLVWNSGTGGTTGT 75
           +DNG+L+V     DG+   +     + +  +     D  +N   Y D          +G 
Sbjct: 40  LDNGLLKVRFG-ADGSAKEVWKGGTNLISRLSGAARDPDKNRSFYLDYY--------SGG 99

Query: 76  FDEMKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHL 135
            +E   +  EVI Q  +Q+ L++       D+   L ++   IM R+ SG YSY +  + 
Sbjct: 100 VNEFVPERLEVIKQTPDQVHLAY-----IDDQNGKLRLEYHLIMTRDVSGLYSYVVAANT 159

Query: 136 KEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNP 195
               P  + + R V+  R D      + ++ +R  PL  D L    K  D  E  +L + 
Sbjct: 160 GS-APVTVSELRNVY--RFDATRLDTLFNSIRRGTPLLYDELEQLPKVQD--ETWRLPD- 219

Query: 196 KEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVG 255
                 G V  KY ++   ++ +   W  M    G W +  S E+ SG  LKQ L  H  
Sbjct: 220 ------GSVYSKYDFAGYQRESRY--WGVMGNGYGAWMVPASGEYYSGDALKQELLVHQD 279

Query: 256 PYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTK 315
              L     +H+   D+V +      ++K++GP  LY+N     G+   L  D   +   
Sbjct: 280 AIILNYLTGSHFGTPDMVAQ----PGFEKLYGPWLLYINQ----GNDRELVADVSRRAEH 339

Query: 316 EVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQT 375
           E  SWPY +   +D   P QR  ++G+L          +T A  A V L +  E  ++  
Sbjct: 340 ERASWPYRW--LDDARYPRQRATVSGRL----------RTEAPHATVVLNSSAE--NFDI 399

Query: 376 ENKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPG--FIGDYRYDVIVSISAGSDMDVGD 435
           +  GY F  +T+ DG FS+ NV  G+Y L A+  G   IG      +     G    +G 
Sbjct: 400 QTTGYLFSARTNRDGRFSLSNVPPGEYRLSAYADGGTQIGLLAQQTV--RVEGKKTRLGQ 459

Query: 436 LVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELY 495
           +    P   P  W IG  DR A EF   D                + RQY    R+    
Sbjct: 460 IDARQP--APLAWAIGQADRRADEFRFGD----------------KPRQY----RWQTEV 519

Query: 496 PDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALAT 555
           P ADL + +G S   KDW++A              +W I F     ++   YTL +A+A 
Sbjct: 520 P-ADLTFEIGKSRERKDWYYAQTQ---------PGSWHILFNTRTPEQ--PYTLNIAIAA 577

Query: 556 ANVAELQVNDPEAKPAVFTTGQI------GHDNTIARHGIH-GLYRLYSVDMSGSLLVEG 615
           A+   +       + AV   GQ+       +D +I R  +  G Y    + +    L +G
Sbjct: 580 ASNNGMTTPASSPQLAVKLNGQLLTTLKYDNDKSIYRGAMQSGRYHEAHIPLPAGALQQG 577

Query: 616 ENTIFLSQIISSSSFNGVMYDYIRLEAPP 631
            N I L  +        VMYD I L   P
Sbjct: 640 GNRITLELLGGM-----VMYDAITLTETP 577

BLAST of MS008337 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 104.4 bits (259), Expect = 4.9e-21
Identity = 152/681 (22.32%), Postives = 257/681 (37.74%), Query Frame = 0

Query: 11  ELQVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWN-SG--TGGT 70
           +  +V++N  L+ T  K  G++  +  +  D +           Y D     SG  T G+
Sbjct: 26  DTSIVLENDRLRATFDKGRGSIIDLYLDGQDFLGPQSGSTGIGPYLDCYCTPSGFYTAGS 85

Query: 71  TGTFDEM------KGKEFEVIVQNEEQIEL--SFKRNYSFKDKAVPLNIDKRFIMLRNSS 130
           T    E+       G ++  I+ N+        F++ +  +D                 +
Sbjct: 86  TNPVTELVQGTDSTGTKYAGIILNDTYTPTGQEFQQYWFLRD---------------GET 145

Query: 131 GFYSYAIYEHLKEWPPF--NLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGK 190
           GF+ ++   +  E  PF  NL + R +F+   D + ++  +D   +  PLP D      +
Sbjct: 146 GFHMFSRLAYYNETTPFLRNLQELRTLFRPNTDLWTHLTSSD--LQTAPLPSDEA--IAE 205

Query: 191 TLDFPEAVKLVN--PKEPQFK--GEVDDKYQYSCENKDLKVHGW-----ISMDPAVGFWQ 250
            +   +A   +N  P +  ++   E   KY +S   +D  VHG       S     G W 
Sbjct: 206 QIVVQDATWRLNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSDGTTYGAWL 265

Query: 251 ITPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYL 310
           +  + +   GGPL  +LT     Y    ++ +++ GE      N    + + FGP F   
Sbjct: 266 VMNTKDTYYGGPLHSDLTVDGIIY---NYIVSNHHGEGTP---NITNGFDRTFGPQFYLF 325

Query: 311 NSVTDGGDPLSLWKDAKTQMTKEVQSWPYSFPAS-----EDFPPPNQRGRLTGKLFVLDR 370
           N    GG   SL +      +    SW   F  S       + P +QRG + G + +   
Sbjct: 326 N----GGGSSSLEELRDEARSLASPSWNADFYDSIAKHVIGYVPSSQRGSVKGTIKLPKN 385

Query: 371 YVSQDQTPATDAYV---GLAAPGEAGSWQTENKGYQFWTKTDADGSFSIENVRIGDYNLY 430
             S       D +      A P            +Q+W   D +G FSI+ V  G Y L 
Sbjct: 386 AKSPIAVLTVDGHYFQDNSAVP----------SSHQYWADIDKNGRFSIDRVVAGKYRLT 445

Query: 431 AWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEF-YVPDPD 490
            +  G  GD+  D IV + A     + +  ++P   G  +W +G PD+S+ EF +    D
Sbjct: 446 VYADGIFGDFTRDGIV-VKARKSTSIKE-TWKPESAGTEIWRLGTPDKSSGEFRHGAARD 505

Query: 491 PNYINKLYVDHPDSRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAH--VNRTRND 550
           P +       HP      +G ++  ++     D  Y +G SD   D+   H  V     D
Sbjct: 506 PTHPR-----HPPEYLIYWGAYDWQSDFPGGID--YMIGESDPATDFNTVHWAVFGPTPD 565

Query: 551 G----SHLSTTWQIKFTVDI----VDKNGAYTLRVALATA--------NVAELQVNDP-- 610
                S+ +  W+I+F +        K    T+++A A A        N +E   N P  
Sbjct: 566 NPVAESNTTHDWRIRFDLSAKQLHARKTATLTIQLAGAKAASGNTDVYNASEPYANLPLR 625

Query: 611 -----EAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQIISSS 628
                + +P     G     + I R  +         +   S L EG N + LS   + +
Sbjct: 626 SYINEQEEPLTMVIGYDQSSSCIVRSAVSCYQVREKWEFPASWLKEGSNLLRLSLPTNGT 658

BLAST of MS008337 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 9.3e-20
Identity = 111/462 (24.03%), Postives = 188/462 (40.69%), Query Frame = 0

Query: 119 SGFYSYAIYEHLKEWPPF--NLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRG 178
           +G ++++   +  E  P+  NL + R +F+   + + +  ++ +  +  PLP  +     
Sbjct: 131 TGLHTFSRLAYYNETTPYLRNLQEFRTLFRPNTELWTH--LSSSEVQTAPLPSKKAVEEE 190

Query: 179 KTLDFPEAVKLVNPKEPQFKGEVD--DKYQYSCENKDLKVHGW-----ISMDPAVGFWQI 238
             +          P +  +    D   KY +S   +D  VHG       S     G W +
Sbjct: 191 VVVQDATWTFNNTPTDEYYVQFADYFTKYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLV 250

Query: 239 TPSSEFRSGGPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLN 298
             + +   GGPL  +LT     Y    +L +++ GE      N    + + FGP + + N
Sbjct: 251 MNTKDTYYGGPLHSDLTVDGIVY---NYLVSNHHGEGTP---NITYGFDRTFGPQYYHFN 310

Query: 299 SVTDGGDPLSLWKDAKTQMTKEVQSWPYSFPAS-----EDFPPPNQRGRLTGKLFVLDRY 358
                G   SL +      T    SW   F  S       + P +QRG + GK+ +    
Sbjct: 311 G--GKGSTASLQELKSDAETLADPSWNVDFYDSIAKHVVGYTPSSQRGSVQGKIKL---- 370

Query: 359 VSQDQTPATDAYVGLAAPGEAGSWQTEN-KGYQFWTKTDADGSFSIENVRIGDYNLYAWV 418
                  AT     L   G+     + N   YQ+W + D  G FS+++V+ G Y L  + 
Sbjct: 371 ----PKGATRPIAVLTVDGQYFQDNSVNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYA 430

Query: 419 PGFIGDYRYDVIVSISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEF-YVPDPDPNY 478
            G  GD+  D  V + AG    + +  +E    G  +W +G PD+S+ EF +    DP  
Sbjct: 431 DGIFGDFVRDG-VQVKAGKKTTIQE-TWEAESAGTEIWRLGTPDKSSGEFRHGVARDPT- 490

Query: 479 INKLYVDHPDSRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAHVN---------R 538
               +  HP      +G ++  ++ +PD  + YT+G SD   D    H +         R
Sbjct: 491 ----HPLHPPEYLIYWGAYDWQSD-FPDG-INYTIGTSDPATDLNTVHWSVFGPTPNDPR 550

Query: 539 TRNDGSHLSTTWQIKFTV---DIVDKNGAYTLRVALATANVA 553
              D +H    W I F +   D+ +++ A TL + LA A  A
Sbjct: 551 VEYDTTH---DWTINFPLSEDDLAERSKA-TLTIQLAGAKAA 561

BLAST of MS008337 vs. ExPASy Swiss-Prot
Match: B8NCU7 (Probable rhamnogalacturonate lyase A OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglA PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 9.3e-12
Identity = 93/347 (26.80%), Postives = 141/347 (40.63%), Query Frame = 0

Query: 282 GPVFLYLNSVTDGGDPLSLW-----KDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTG 341
           GP F  +NS  +GG   SL+        +T+  ++    PYS   S    P         
Sbjct: 205 GPFFRDINS-NNGGSYNSLYWYMNSGHVQTEDRRQGLHGPYSMYFSRSGTPSTDIDTSFF 264

Query: 342 KLFVLDRYVSQDQTPATDAYVGLAAPGEAG-SWQTE--NKGYQFWTKTDADGSFSIENVR 401
               +  YV+ D         G A+  ++   W     N   Q+WT T +DGSF+   ++
Sbjct: 265 ANLDIKGYVAAD---GRGTVSGTASGADSSFKWVVHWYNADAQYWTYTSSDGSFTSPAMK 324

Query: 402 IGDYNLYAWVPGFIGDYRY-DVIVSISAGS--DMDVGDLVYEPPRNGPTLWEIGIPDRSA 461
            GDY +      + G+Y+  +  VS++AGS    D+   V      G T+++IG  D + 
Sbjct: 325 PGDYTMVY----YQGEYKVAETSVSVTAGSSTSKDISGFV----ETGDTIFKIGDWDGTP 384

Query: 462 AEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAH 521
             F       N  N+L +   DSR   +G             L YTVG S+ T D+  A 
Sbjct: 385 TGF------RNAENQLRMHPSDSRMSSWG------------PLTYTVGSSELT-DFPMAA 444

Query: 522 VNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALATANVA---ELQVNDPEAKPAVFT 581
                +  +       IKFT     + GA TLR+    +      +  +ND E       
Sbjct: 445 FKGVNDPVT-------IKFTATSA-QTGAATLRIGTTLSFAGGRPQATINDYEGSAPSAP 504

Query: 582 TGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQIISSS 615
           T    +   + R    GL  +Y V++    +VEGENTI +S I  SS
Sbjct: 505 TNL--NSRGVTRGAYRGLGEVYDVNIPSGTIVEGENTITISVISGSS 510

BLAST of MS008337 vs. ExPASy Swiss-Prot
Match: Q2U0Q1 (Probable rhamnogalacturonate lyase A OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=rglA PE=3 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-11
Identity = 90/347 (25.94%), Postives = 139/347 (40.06%), Query Frame = 0

Query: 282 GPVFLYLNSVTDGGDPLSLW-----KDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTG 341
           GP F  +NS  +GG   SL+        +T+  ++    PYS   S    P         
Sbjct: 205 GPFFRDINS-NNGGSYNSLYWYMNSGHVQTEDRRQGLHGPYSMYFSRSGTPSTDIDTSFF 264

Query: 342 KLFVLDRYVSQDQTPATDAYVGLAAPGEAG-SWQTE--NKGYQFWTKTDADGSFSIENVR 401
               +  YV+ D         G A+  ++   W     N   Q+WT T +DGSF+   ++
Sbjct: 265 ANLDIKGYVATD---GRGTVSGTASGADSSFKWVVHWYNADAQYWTYTSSDGSFTSPAMK 324

Query: 402 IGDYNLYAWVPGFIGDYRY---DVIVSISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSA 461
            GDY +      + G+Y+     V V++ + +  D+   V      G T+++IG  D + 
Sbjct: 325 PGDYTMVY----YQGEYKVAETSVSVTVGSSTSKDISGSV----ETGDTIFKIGDWDGTP 384

Query: 462 AEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADLVYTVGVSDYTKDWFFAH 521
             F       N  N+L +   DSR   +G             L YTVG S+ T D+  A 
Sbjct: 385 TGF------RNAENQLRMHPSDSRMSSWG------------PLTYTVGSSELT-DFPMAA 444

Query: 522 VNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALATANVA---ELQVNDPEAKPAVFT 581
                +  +       IKFT     + GA TLR+    +      +  +ND E       
Sbjct: 445 FKGVNDPVT-------IKFTATSA-QTGAATLRIGTTLSFAGGRPQATINDYEGSAPSAP 504

Query: 582 TGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQIISSS 615
           T    +   + R    GL  +Y V++    +VEGENTI +S I  SS
Sbjct: 505 TNL--NSRGVTRGAYRGLGEVYDVNIPSGTIVEGENTITISVISGSS 510

BLAST of MS008337 vs. ExPASy TrEMBL
Match: A0A6J1C5C7 (Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111008520 PE=3 SV=1)

HSP 1 Score: 1287.3 bits (3330), Expect = 0.0e+00
Identity = 617/623 (99.04%), Postives = 617/623 (99.04%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE
Sbjct: 15  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 74

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW
Sbjct: 75  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 134

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP
Sbjct: 135 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 194

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 253
           QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA
Sbjct: 195 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 254

Query: 254 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 313
           LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ
Sbjct: 255 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 314

Query: 314 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 373
           SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK
Sbjct: 315 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 374

Query: 374 GYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 433
           GYQFWTKTDADGSFSIEN RIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP
Sbjct: 375 GYQFWTKTDADGSFSIENARIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 434

Query: 434 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADL 493
           PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPD RFRQYGLWERYAELYPDADL
Sbjct: 435 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPD-RFRQYGLWERYAELYPDADL 494

Query: 494 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALATANVAE 553
           VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD VDKN AYTLRVALATANVAE
Sbjct: 495 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDNVDKNDAYTLRVALATANVAE 554

Query: 554 L--QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQII 613
           L  QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQII
Sbjct: 555 LQIQVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQII 614

Query: 614 SSSSFNGVMYDYIRLEAPPSDNP 635
           SSSSFNGVMYDYIRLEAPPSDNP
Sbjct: 615 SSSSFNGVMYDYIRLEAPPSDNP 636

BLAST of MS008337 vs. ExPASy TrEMBL
Match: A0A1S3CQT8 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503646 PE=3 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 569/623 (91.33%), Postives = 602/623 (96.63%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLVWNSGT GTTGTFDE
Sbjct: 15  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKETHRGYWDLVWNSGTSGTTGTFDE 74

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           M+GKEFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEH++EW
Sbjct: 75  MRGKEFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHMREW 134

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK LDFPEAV LVNPKEP
Sbjct: 135 PPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEP 194

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 253
           +FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGGPLKQNLTSHVGPYA
Sbjct: 195 EFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 254

Query: 254 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 313
           LAMFLSAHYSGEDLVLKL+PDEPWKKVFGPVF YLNSV+DGGDPLSLW+DAKTQM+ EVQ
Sbjct: 255 LAMFLSAHYSGEDLVLKLSPDEPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQMSIEVQ 314

Query: 314 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 373
           SWPYSFPASEDFPPPNQRGRLTGKLFVLDRY+++++ PATDAYVGLAAPGEAGSWQTENK
Sbjct: 315 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYINEEKMPATDAYVGLAAPGEAGSWQTENK 374

Query: 374 GYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 433
           GYQFWTKTDADGSFSIE+VRIGDYNLYAWVPGFIGDYRYDV++++S GSDMDVGDLVYEP
Sbjct: 375 GYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVSPGSDMDVGDLVYEP 434

Query: 434 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADL 493
           PRNGPTLWEIGIPDRSAAEFY+PDPDP YINKLYV+HPD RFRQYGLWERYAELYPDADL
Sbjct: 435 PRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYVNHPD-RFRQYGLWERYAELYPDADL 494

Query: 494 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD-IVDKNGAYTLRVALATANVA 553
           VY+VGVSDYTKDWFFAHVNR RNDGSHL+TTW+IKF VD I+DK+G YTLRVALATANVA
Sbjct: 495 VYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRVDNIIDKSGTYTLRVALATANVA 554

Query: 554 EL--QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQI 613
           EL  QVN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSVD+ GSLLVEGENTIFLSQI
Sbjct: 555 ELQIQVNNPEAKPSLFTTGMIGHDNAIARHGIHGLYRLYSVDILGSLLVEGENTIFLSQI 614

Query: 614 ISSSSFNGVMYDYIRLEAPPSDN 634
           I+SSSFNGVMYDYIRLE PPSD+
Sbjct: 615 INSSSFNGVMYDYIRLEGPPSDS 636

BLAST of MS008337 vs. ExPASy TrEMBL
Match: A0A0A0LGY0 (Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892750 PE=3 SV=1)

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 559/622 (89.87%), Postives = 600/622 (96.46%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKE HRGYWDLVWNSGT GTTGTFDE
Sbjct: 15  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKEAHRGYWDLVWNSGTPGTTGTFDE 74

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           M+G+EFEV+VQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEH++EW
Sbjct: 75  MRGREFEVMVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHMREW 134

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIAD+RQRFMPLPEDRLRHRGK LDFPEAV LVNPKEP
Sbjct: 135 PPFNLPQTRIVFKLRKDKFHYMAIADDRQRFMPLPEDRLRHRGKPLDFPEAVLLVNPKEP 194

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 253
           +FKGEVDDKYQYSCENKDLKVHGWISMDP VGFWQITPSSEFRSGGPLKQNLTSHVGPYA
Sbjct: 195 EFKGEVDDKYQYSCENKDLKVHGWISMDPPVGFWQITPSSEFRSGGPLKQNLTSHVGPYA 254

Query: 254 LAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKEVQ 313
           LAMFLSAHYSGEDLVLK++PDEPWKKVFGPVF YLNSV+DGGDPLSLW+DAKTQM+KEVQ
Sbjct: 255 LAMFLSAHYSGEDLVLKVSPDEPWKKVFGPVFFYLNSVSDGGDPLSLWEDAKTQMSKEVQ 314

Query: 314 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTENK 373
           SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYV++++ PATDAYVGLAAPG+ GSWQTE K
Sbjct: 315 SWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVNEEKMPATDAYVGLAAPGDVGSWQTEKK 374

Query: 374 GYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVYEP 433
           GYQFWTKTDADGSFSIE+VRIGDYNLYAWVPGFIGDYRYDV++++S+GSDMDVGDLVYEP
Sbjct: 375 GYQFWTKTDADGSFSIEHVRIGDYNLYAWVPGFIGDYRYDVLINVSSGSDMDVGDLVYEP 434

Query: 434 PRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDADL 493
           PRNGPTLWEIGIPDRSAAEFY+PDPDP YINKLY++HPD RFRQYGLWE+YAEL+PDADL
Sbjct: 435 PRNGPTLWEIGIPDRSAAEFYIPDPDPKYINKLYINHPD-RFRQYGLWEKYAELHPDADL 494

Query: 494 VYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD-IVDKNGAYTLRVALATANVA 553
           VY+VGVSDYTKDWFFAHVNR RNDGSHL+TTW+IKF +D I+DK  +YTLRVALATANVA
Sbjct: 495 VYSVGVSDYTKDWFFAHVNRKRNDGSHLATTWEIKFRLDNIIDKGVSYTLRVALATANVA 554

Query: 554 EL--QVNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQI 613
           EL  QVN+PEAKP++FTTG IGHDN IARHGIHGLYRLYSVD+ GSLLVEGENTIFLSQI
Sbjct: 555 ELQIQVNNPEAKPSLFTTGVIGHDNAIARHGIHGLYRLYSVDILGSLLVEGENTIFLSQI 614

Query: 614 ISSSSFNGVMYDYIRLEAPPSD 633
           I++SSFNG+MYDYIRLE PPSD
Sbjct: 615 INNSSFNGIMYDYIRLEGPPSD 635

BLAST of MS008337 vs. ExPASy TrEMBL
Match: A0A6J1IFC5 (Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111476434 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 546/626 (87.22%), Postives = 583/626 (93.13%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGIL VTLSKPDGNVTGIKY++IDN+LEV DKE HRGYWDLVWNSGT GTTGTFDE
Sbjct: 16  VVMDNGILAVTLSKPDGNVTGIKYSNIDNILEVRDKEAHRGYWDLVWNSGTPGTTGTFDE 75

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           M+GKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNSSGFYSYAIYEHL+EW
Sbjct: 76  MRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNSSGFYSYAIYEHLREW 135

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKL KDKFHYMAIADNR+R MPLP+DR+  RGKTLDFPEAV LV+P+E 
Sbjct: 136 PPFNLPQTRIVFKLSKDKFHYMAIADNRKRSMPLPDDRVGRRGKTLDFPEAVLLVHPREA 195

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPA----VGFWQITPSSEFRSGGPLKQNLTSHV 253
            FKGEVDDKYQYSCENKDLKVHGWISMDP+    VGFWQITPSSEFRSGGPLKQNLTSHV
Sbjct: 196 AFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHV 255

Query: 254 GPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMT 313
           GPYALAMFLSAHY GE+LVLK+NPDEPWKKVFGPVF YLNSV +G DPLSLW+DAKTQMT
Sbjct: 256 GPYALAMFLSAHYGGEELVLKMNPDEPWKKVFGPVFFYLNSVPNGTDPLSLWEDAKTQMT 315

Query: 314 KEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQ 373
           KEVQSWPYSFPASE+FPPPNQRGRLTG LFVLDRYV+Q+Q PA +AYVGLAAPG+AGSWQ
Sbjct: 316 KEVQSWPYSFPASENFPPPNQRGRLTGNLFVLDRYVNQEQMPAAEAYVGLAAPGDAGSWQ 375

Query: 374 TENKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDL 433
           TE KGYQFWTKTDA GSFSIENVRIGDYNLYAWVPGFIGDY YDV++S+SAGSD+D+GDL
Sbjct: 376 TETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYHYDVVISVSAGSDIDIGDL 435

Query: 434 VYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYP 493
           V+EPPRNGPT+WEIGIPDRSA EFY+PDP+PNYINKLYVDHPD RFRQYGLWERYAELYP
Sbjct: 436 VFEPPRNGPTVWEIGIPDRSANEFYIPDPNPNYINKLYVDHPD-RFRQYGLWERYAELYP 495

Query: 494 DADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD-IVDKNGAYTLRVALAT 553
           D DLVYTVGVSDY KDWFFAHVNRTRNDGSHL TTWQIKFT+D  VDK+G Y LRVALAT
Sbjct: 496 DTDLVYTVGVSDYPKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNVDKSGIYKLRVALAT 555

Query: 554 ANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIF 613
           ANVAELQ  VN+ E + A+FTTGQIGHDN IARHGIHGLYRLYSVDMSGS+LVEGENTIF
Sbjct: 556 ANVAELQIRVNNLEVEHALFTTGQIGHDNAIARHGIHGLYRLYSVDMSGSVLVEGENTIF 615

Query: 614 LSQIISSSSFNGVMYDYIRLEAPPSD 633
           LSQII+ SSFNGVMYDYIRLE PPSD
Sbjct: 616 LSQIINDSSFNGVMYDYIRLEGPPSD 640

BLAST of MS008337 vs. ExPASy TrEMBL
Match: A0A6J1FDW4 (Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111443196 PE=3 SV=1)

HSP 1 Score: 1149.4 bits (2972), Expect = 0.0e+00
Identity = 548/626 (87.54%), Postives = 583/626 (93.13%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGIL VTLSKP GNVTGIKY+SIDNVLEV DKE HRGYWDLVWNSGT GTTGTFDE
Sbjct: 16  VVMDNGILAVTLSKPYGNVTGIKYSSIDNVLEVRDKEAHRGYWDLVWNSGTPGTTGTFDE 75

Query: 74  MKGKEFEVIVQNEEQIELSFKRNYSFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 133
           M+GKEFEV+VQNEEQIELSFKRNYSFKDK VPLNIDKRFIMLRNSSGFYSYAIYEHL+EW
Sbjct: 76  MRGKEFEVVVQNEEQIELSFKRNYSFKDKEVPLNIDKRFIMLRNSSGFYSYAIYEHLREW 135

Query: 134 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 193
           PPFNLPQTRIVFKLRKDKFHYMAIADNR+R MPLP+DR+  RGKTLDFPEAV LV+P+E 
Sbjct: 136 PPFNLPQTRIVFKLRKDKFHYMAIADNRKRSMPLPDDRVGGRGKTLDFPEAVLLVHPREA 195

Query: 194 QFKGEVDDKYQYSCENKDLKVHGWISMDPA----VGFWQITPSSEFRSGGPLKQNLTSHV 253
            FKGEVDDKYQYSCENKDLKVHGWISMDP+    VGFWQITPSSEFRSGGPLKQNLTSHV
Sbjct: 196 AFKGEVDDKYQYSCENKDLKVHGWISMDPSDDPPVGFWQITPSSEFRSGGPLKQNLTSHV 255

Query: 254 GPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMT 313
           GPYALAMFLSAHY GE+LVLKLN DEPWKKVFGPVF YLNSV +G DPL LW+DAK QMT
Sbjct: 256 GPYALAMFLSAHYGGEELVLKLNADEPWKKVFGPVFFYLNSVPNGRDPLCLWEDAKIQMT 315

Query: 314 KEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQ 373
           KEVQSWPYSFPAS++FPPPNQRGRLTG L+VLD YV+Q+Q PA +AYVGLAAPG+AGSWQ
Sbjct: 316 KEVQSWPYSFPASQNFPPPNQRGRLTGNLYVLDTYVNQEQMPAAEAYVGLAAPGDAGSWQ 375

Query: 374 TENKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDL 433
           TE KGYQFWTKTDA GSFSIENVRIGDYNLYAWVPGFIGDYRYDV++S+SAGSDMDVGDL
Sbjct: 376 TETKGYQFWTKTDAYGSFSIENVRIGDYNLYAWVPGFIGDYRYDVVISVSAGSDMDVGDL 435

Query: 434 VYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYP 493
           V+EPPRNGPTLWEIGIPDRSAAEFY+PDP+PNYINKLYV HPD RFRQYGLWERYAELYP
Sbjct: 436 VFEPPRNGPTLWEIGIPDRSAAEFYIPDPNPNYINKLYVHHPD-RFRQYGLWERYAELYP 495

Query: 494 DADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVD-IVDKNGAYTLRVALAT 553
           D DLVYTVGVSDYTKDWFFAHVNRTRNDGSHL TTWQIKFT+D  +DK+G Y LRVALAT
Sbjct: 496 DTDLVYTVGVSDYTKDWFFAHVNRTRNDGSHLPTTWQIKFTLDNNIDKSGIYKLRVALAT 555

Query: 554 ANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIF 613
           ANVAELQ  VN+PE +PA+FTTGQIGHDN IARHGIHGLYRLYSVDMS S+LVEGENTIF
Sbjct: 556 ANVAELQIRVNNPEVEPALFTTGQIGHDNAIARHGIHGLYRLYSVDMSRSVLVEGENTIF 615

Query: 614 LSQIISSSSFNGVMYDYIRLEAPPSD 633
           LSQII++SSFNGVMYDYIRLE PPSD
Sbjct: 616 LSQIINTSSFNGVMYDYIRLEGPPSD 640

BLAST of MS008337 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 874.4 bits (2258), Expect = 5.6e-254
Identity = 413/641 (64.43%), Postives = 510/641 (79.56%), Query Frame = 0

Query: 2   QDDVLCMCGELQVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVW- 61
           QD V     E  VVM NG ++VT+SKPDG VTGI Y  +DN+LE  +++ +RGYWDLVW 
Sbjct: 4   QDSVQLDVQESHVVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDLVWS 63

Query: 62  NSGTGGTTGTFDEMKGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNS 121
           + GT GTTG  + +KG  FEV+V+NEE +E+SF R +  S +D   P+N+DKRFIM ++ 
Sbjct: 64  DEGTPGTTGKSERIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMRKDV 123

Query: 122 SGFYSYAIYEHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKT 181
           +GFYSYAI+EHL EWP FNLPQTRIV+KLRKDKF YMAIADNRQR MPLPEDRL  RG+ 
Sbjct: 124 TGFYSYAIFEHLAEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKRGRP 183

Query: 182 LDFPEAVKLVNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSG 241
           L +PEAV LV+P E +FKGEVDDKY+YS ENKDLKVHGWIS +  +G WQI PS+EFRSG
Sbjct: 184 LAYPEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKVHGWISHNLDLGCWQIIPSNEFRSG 243

Query: 242 GPLKQNLTSHVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTD-GGDP 301
           G  KQNLTSHVGP +LAMFLSAHY+GED+V+K+   + WKKVFGPVF YLN + D   DP
Sbjct: 244 GLSKQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDKTSDP 303

Query: 302 LSLWKDAKTQMTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYV 361
           LSLW+DAK QM  EVQSWPY FPASEDFP  ++RG ++G+L V D+++S D  PA  A+V
Sbjct: 304 LSLWQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPANGAFV 363

Query: 362 GLAAPGEAGSWQTENKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVS 421
           GLA PGE GSWQ E+KGYQFWT+ D+DG F+I ++R G+YNL  +V G+IGDY+Y+ +++
Sbjct: 364 GLAPPGEVGSWQLESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYEQLIN 423

Query: 422 ISAGSDMDVGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQ 481
           I+AG D+DVG++VYEPPR+GPT+WEIGIPDRSAAEF+VPDP+P YINKLY+ HPD RFRQ
Sbjct: 424 ITAGCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPD-RFRQ 483

Query: 482 YGLWERYAELYPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKN 541
           YGLWERY ELYP  DLV+T+GVSDY KDWFFAHV R   D ++  TTWQIKF ++ V K+
Sbjct: 484 YGLWERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENVQKS 543

Query: 542 GAYTLRVALATANVAELQV----NDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDM 601
             Y +R+ALATANVAELQV    +D E    +FTTG IGHDN IARHGIHG+YRLY+VD+
Sbjct: 544 CTYKIRIALATANVAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYNVDV 603

Query: 602 SGSLLVEGENTIFLSQIISSS-SFNGVMYDYIRLEAPPSDN 634
               LVEG+NT+FL+Q ++++ +FNG+MYDYIRLE PP D+
Sbjct: 604 PSEKLVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGPPLDS 643

BLAST of MS008337 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 870.5 bits (2248), Expect = 8.0e-253
Identity = 419/620 (67.58%), Postives = 497/620 (80.16%), Query Frame = 0

Query: 16  MDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDEMK 75
           MDNGI +VTLSKPDG VTGI+YN IDN+LEV ++E +RGYWDLVW  G  GT G FD +K
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVW--GGSGTAGGFDVIK 60

Query: 76  GKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLKEW 135
           G  FEVIV+NEEQIELSF R +  S + KAVPLNIDKRF+ML  SSGFY+YAIYEHLKEW
Sbjct: 61  GSNFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEW 120

Query: 136 PPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPKEP 195
           P F+L +TRI FKLRK+KFHYMA+ D+RQRFMPLP+DRL  RG+ L +PEAV LVNP E 
Sbjct: 121 PAFSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLES 180

Query: 196 QFKGEVDDKYQYSCENKDLKVHGWISMD-PAVGFWQITPSSEFRSGGPLKQNLTSHVGPY 255
           QFKGEVDDKYQYSCENKD+ VHGWI  + P+VGFW ITPS E+R+GGP KQNLTSHVGP 
Sbjct: 181 QFKGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPT 240

Query: 256 ALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVT-DGGDPLSLWKDAKTQMTKE 315
           ALA+F+SAHY+GEDLV K +  E WKKVFGPVF+YLNS T D  DPL LW+DAK+QM  E
Sbjct: 241 ALAVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVE 300

Query: 316 VQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTE 375
            +SWPYSFPAS+D+    QRG + G+L V DRYV +D   A   YVGLA PG AGSWQ E
Sbjct: 301 AESWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRE 360

Query: 376 NKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVY 435
            K YQFWT+TD +G F I  +R G YNLYAW+PGFIGDY+YD +++I++G  + V DLVY
Sbjct: 361 CKEYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVY 420

Query: 436 EPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDA 495
           +PPRNG TLWEIG PDRSAAEFYVPDP+P YIN LY +HPD RFRQYGLWERYAELYPD 
Sbjct: 421 QPPRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPD-RFRQYGLWERYAELYPDK 480

Query: 496 DLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALATANV 555
           DLVY VG SDY KDWF+A V R +++ ++  TTWQIKF +  +DKN +YTLRVA+A+A  
Sbjct: 481 DLVYVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATF 540

Query: 556 AELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQ 615
           +ELQ  VN+  A P +FT+G IG DN+IARHGIHGLY L++V+++GS L+EGENT+FL+Q
Sbjct: 541 SELQIRVNNANASP-MFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQ 600

Query: 616 IISSSSFNGVMYDYIRLEAP 630
             S+S F G+MYDYIR EAP
Sbjct: 601 PRSTSPFQGIMYDYIRFEAP 616

BLAST of MS008337 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 828.9 bits (2140), Expect = 2.7e-240
Identity = 390/622 (62.70%), Postives = 477/622 (76.69%), Query Frame = 0

Query: 14  VVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTGTFDE 73
           VVMDNGILQVTLSKP G +TGI+YN IDNVLEV +KE +RGYWDL WN    G  G FD 
Sbjct: 58  VVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKETNRGYWDLHWNE--PGGKGIFDV 117

Query: 74  MKGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGFYSYAIYEHLK 133
           + G  F VIV+ EEQ+E+SF R +  S + K +PLNIDKRFIMLR SSG YSY IYEHLK
Sbjct: 118 ISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKRFIMLRGSSGVYSYGIYEHLK 177

Query: 134 EWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNPK 193
           +WP F L +TRI FKLRKDKFHYMA+AD+R+R MP P+D  + R +TLD+ EA  L  P 
Sbjct: 178 DWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDLCKGRCQTLDYQEASLLTAPC 237

Query: 194 EPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVGP 253
           +P+ +GEVDDKYQYSCENKDL+VHGWIS DP VGFWQITPS+EFRSGGPLKQNLTSHVGP
Sbjct: 238 DPRLQGEVDDKYQYSCENKDLRVHGWISFDPPVGFWQITPSNEFRSGGPLKQNLTSHVGP 297

Query: 254 YALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTKE 313
             LA+F S HY+G+ ++ +    EPWKKV+GPVF+YLNS  +G DPL LW DAK +M  E
Sbjct: 298 TTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNSTANGDDPLCLWDDAKIKMMAE 357

Query: 314 VQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQTE 373
           V+ WPYSF AS+D+P   +RG   G+L + DR+++ D   A  AYVGLA PG++GSWQ E
Sbjct: 358 VERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLISARGAYVGLAPPGDSGSWQIE 417

Query: 374 NKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLVY 433
            KGYQFW   D  G FSI NVR G+YNLYAWVP FIGDY    IV +++G  +++GD+VY
Sbjct: 418 CKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDYHNGTIVRVTSGCMIEMGDIVY 477

Query: 434 EPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPDA 493
           EPPR+GPTLWEIGIPDR A+EF++PDPDP  +N++ V H D RFRQYGLW++Y ++YP+ 
Sbjct: 478 EPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHHQD-RFRQYGLWKKYTDMYPND 537

Query: 494 DLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALATANV 553
           DLVYTVGVSDY +DWFFAHV R + D  H  TTWQI F ++ +D+   Y LRVA+A+A +
Sbjct: 538 DLVYTVGVSDYRRDWFFAHVPRKKGD-VHEGTTWQIIFNLENIDQKANYKLRVAIASATL 597

Query: 554 AELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIFLSQ 613
           AELQ  +ND EA   +FTTG IG DN+IARHGIHG+Y LY+V++ G+ LV+G+NTIFL Q
Sbjct: 598 AELQIRINDAEAIRPLFTTGLIGRDNSIARHGIHGVYMLYAVNIPGNRLVQGDNTIFLKQ 657

Query: 614 IISSSSFNGVMYDYIRLEAPPS 632
              +  F G+MYDYIRLE PPS
Sbjct: 658 PRCNGPFQGIMYDYIRLEGPPS 675

BLAST of MS008337 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 798.1 bits (2060), Expect = 5.1e-231
Identity = 382/627 (60.93%), Postives = 475/627 (75.76%), Query Frame = 0

Query: 16  MDNGILQVTLSKPDGNVTGIKYNSIDNVLE-VDDKENHRGYWDLVWN---SGTGGTTGTF 75
           M+N  LQ+TLS P+G VTGI+YN IDNVL    +KE  RGYWD+VWN        T GT 
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 76  DEMKGKEFEVIVQNEEQIELSFKRNYSFKD-KAVPLNIDKRFIMLRNSSGFYSYAIYEHL 135
           D ++  + EVI QN+E+IELSF R ++     AVP+NIDKRF+ML+NSSGFYSYAI+E L
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTSSTTAVPVNIDKRFVMLQNSSGFYSYAIFERL 120

Query: 136 KEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKLVNP 195
           + WP   L   R+VFKL K KFHYMAI+D+RQR+MP+P+DR+  RG+ L +PEAV+L++P
Sbjct: 121 QGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLDP 180

Query: 196 KEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTSHVG 255
            EP+FKGEVDDKY+YS E+KD+KVHGWIS + +VGFWQITPS+EFRS GPLKQ L SHVG
Sbjct: 181 IEPEFKGEVDDKYEYSMESKDIKVHGWISTNDSVGFWQITPSNEFRSAGPLKQFLGSHVG 240

Query: 256 PYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQMTK 315
           P  LA+F S HY G DL++     E WKKVFGPVF+YLNS   G DPL LW +AK Q   
Sbjct: 241 PTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQTKI 300

Query: 316 EVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGSWQT 375
           E + WPY+F AS+DFP  +QRG ++G+L V DR++S +  PA  +YVGLAAPG+ GSWQ 
Sbjct: 301 EEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSWQR 360

Query: 376 ENKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVGDLV 435
           E KGYQFW+K D +GSFSI NVR G YNLYA+ PGFIGDY  D +  IS GS + +GDLV
Sbjct: 361 ECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGDLV 420

Query: 436 YEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAELYPD 495
           YEPPR+G TLWEIG+PDRSAAEFY+PDP+P+++NKLY++H D ++RQYGLWERY+ELYPD
Sbjct: 421 YEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSD-KYRQYGLWERYSELYPD 480

Query: 496 ADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKN--GAYTLRVALAT 555
            D+VY V + DY+K+WFF  V R + +G +  TTWQI+F  D   KN  G + LR+ALAT
Sbjct: 481 EDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVTGNFKLRIALAT 540

Query: 556 ANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTIF 615
           +NVAELQ  VND  A P +F T QIG DNTIARHGIHGLY LYSV++  + L  G NTI+
Sbjct: 541 SNVAELQVRVNDLSADPPLFRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNNTIY 600

Query: 616 LSQIISSSSFNGVMYDYIRLEAPPSDN 634
           L+Q +++S F G+MYDYIRLE P S N
Sbjct: 601 LTQALATSPFQGLMYDYIRLEYPDSIN 626

BLAST of MS008337 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 760.0 bits (1961), Expect = 1.5e-219
Identity = 370/624 (59.29%), Postives = 455/624 (72.92%), Query Frame = 0

Query: 10  GELQVVMDNGILQVTLSKPDGNVTGIKYNSIDNVLEVDDKENHRGYWDLVWNSGTGGTTG 69
           G   VV+DNGI+QVT S P+G +TGIKY+ IDNVL  DDK + RGYWD+VW         
Sbjct: 58  GHDTVVVDNGIVQVTFSNPEGLITGIKYHGIDNVL--DDKIDDRGYWDVVWYEPE--KKQ 117

Query: 70  TFDEMKGKEFEVIVQNEEQIELSFKRNY--SFKDKAVPLNIDKRFIMLRNSSGFYSYAIY 129
             D+++G +FE+I QNEEQIE+SF R +  S +   VPLN+DKR+I+    SG Y Y I 
Sbjct: 118 KTDKLEGTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSGVSGLYMYGIL 177

Query: 130 EHLKEWPPFNLPQTRIVFKLRKDKFHYMAIADNRQRFMPLPEDRLRHRGKTLDFPEAVKL 189
           E L+ WP  ++ Q RIVFKL   KF +MAI+D+RQR MP   D  R   K+L + EAV L
Sbjct: 178 ERLEGWPDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMAD--RENSKSLAYKEAVLL 237

Query: 190 VNPKEPQFKGEVDDKYQYSCENKDLKVHGWISMDPAVGFWQITPSSEFRSGGPLKQNLTS 249
            NP  P FKGEVDDKY YS E+KD  VHGWIS DP VGFW ITPS EFR GGP+KQ+LTS
Sbjct: 238 TNPSNPMFKGEVDDKYMYSMEDKDNNVHGWISSDPPVGFWMITPSDEFRLGGPIKQDLTS 297

Query: 250 HVGPYALAMFLSAHYSGEDLVLKLNPDEPWKKVFGPVFLYLNSVTDGGDPLSLWKDAKTQ 309
           H GP  L+MF S HY+G+++ +     EPWKKVFGPV  YLNSV+     L LW+DAK Q
Sbjct: 298 HAGPITLSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQ 357

Query: 310 MTKEVQSWPYSFPASEDFPPPNQRGRLTGKLFVLDRYVSQDQTPATDAYVGLAAPGEAGS 369
           M  EV+SWPY F  SED+P  +QRG L G+  + D YVS+ +     A+VGLA  GEAGS
Sbjct: 358 MAAEVKSWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGS 417

Query: 370 WQTENKGYQFWTKTDADGSFSIENVRIGDYNLYAWVPGFIGDYRYDVIVSISAGSDMDVG 429
           WQTE+KGYQFWTK D  G F IENVR G+Y+LYAW  GFIGDY+Y+  ++I+ GS+M+VG
Sbjct: 418 WQTESKGYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVG 477

Query: 430 DLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPDPNYINKLYVDHPDSRFRQYGLWERYAEL 489
            LVYEPPRNGPTLWEIG+PDR+A EFY+PDP P  +NKLYV+    RFRQYGLW+RYA+L
Sbjct: 478 PLVYEPPRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADL 537

Query: 490 YPDADLVYTVGVSDYTKDWFFAHVNRTRNDGSHLSTTWQIKFTVDIVDKNGAYTLRVALA 549
           YP  DLVYT+GVSDY  DWFFAHV R   + ++  TTWQI F +  V++ G YTLR+ALA
Sbjct: 538 YPQNDLVYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALA 597

Query: 550 TANVAELQ--VNDPEAKPAVFTTGQIGHDNTIARHGIHGLYRLYSVDMSGSLLVEGENTI 609
           +A  +ELQ  +NDP++  A+FTTG IG DN IARHGIHGLYRLYS+D++G+LL  G+NTI
Sbjct: 598 SAADSELQIRINDPKS-DAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTI 657

Query: 610 FLSQIISSSSFNGVMYDYIRLEAP 630
           FL+Q  S + F G+MYDYIRLE+P
Sbjct: 658 FLTQTRSRTPFQGIMYDYIRLESP 674

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136955.10.0e+0099.04probable rhamnogalacturonate lyase B [Momordica charantia][more]
XP_008466141.10.0e+0091.33PREDICTED: probable rhamnogalacturonate lyase B [Cucumis melo][more]
XP_038899392.10.0e+0091.48probable rhamnogalacturonate lyase B [Benincasa hispida][more]
XP_004136251.10.0e+0089.87probable rhamnogalacturonate lyase B [Cucumis sativus] >KGN60274.1 hypothetical ... [more]
XP_023535230.10.0e+0088.18probable rhamnogalacturonate lyase B isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8RJP23.0e-3426.07Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE... [more]
Q5AZ854.9e-2122.32Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 ... [more]
A5ABH49.3e-2024.03Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / F... [more]
B8NCU79.3e-1226.80Probable rhamnogalacturonate lyase A OS=Aspergillus flavus (strain ATCC 200026 /... [more]
Q2U0Q13.5e-1125.94Probable rhamnogalacturonate lyase A OS=Aspergillus oryzae (strain ATCC 42149 / ... [more]
Match NameE-valueIdentityDescription
A0A6J1C5C70.0e+0099.04Rhamnogalacturonan endolyase OS=Momordica charantia OX=3673 GN=LOC111008520 PE=3... [more]
A0A1S3CQT80.0e+0091.33Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103503646 PE=3 SV=1[more]
A0A0A0LGY00.0e+0089.87Rhamnogalacturonan endolyase OS=Cucumis sativus OX=3659 GN=Csa_3G892750 PE=3 SV=... [more]
A0A6J1IFC50.0e+0087.22Rhamnogalacturonan endolyase OS=Cucurbita maxima OX=3661 GN=LOC111476434 PE=3 SV... [more]
A0A6J1FDW40.0e+0087.54Rhamnogalacturonan endolyase OS=Cucurbita moschata OX=3662 GN=LOC111443196 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT4G24430.15.6e-25464.43Rhamnogalacturonate lyase family protein [more]
AT1G09890.18.0e-25367.58Rhamnogalacturonate lyase family protein [more]
AT1G09910.12.7e-24062.70Rhamnogalacturonate lyase family protein [more]
AT1G09880.15.1e-23160.93Rhamnogalacturonate lyase family protein [more]
AT2G22620.11.5e-21959.29Rhamnogalacturonate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029411Rhamnogalacturonan lyase, domain IIIPFAMPF14683CBM-likecoord: 439..626
e-value: 1.1E-51
score: 175.1
NoneNo IPR availableGENE3D2.60.120.260coord: 437..627
e-value: 1.1E-17
score: 66.1
NoneNo IPR availableGENE3D2.60.40.1120coord: 327..426
e-value: 1.4E-10
score: 43.3
NoneNo IPR availablePANTHERPTHR32018RHAMNOGALACTURONATE LYASE FAMILY PROTEINcoord: 12..633
NoneNo IPR availablePANTHERPTHR32018:SF16RHAMNOGALACTURONATE LYASE B-RELATEDcoord: 12..633
NoneNo IPR availableCDDcd10320RGL4_Ncoord: 12..300
e-value: 9.27669E-73
score: 233.435
NoneNo IPR availableCDDcd10317RGL4_Ccoord: 441..627
e-value: 1.04043E-55
score: 184.401
IPR014718Glycoside hydrolase-type carbohydrate-bindingGENE3D2.70.98.10coord: 10..291
e-value: 3.7E-10
score: 41.7
IPR010325Rhamnogalacturonate lyasePFAMPF06045Rhamnogal_lyasecoord: 9..199
e-value: 3.4E-86
score: 288.1
IPR029413Rhamnogalacturonan lyase, domain IIPFAMPF14686fn3_3coord: 354..424
e-value: 1.8E-23
score: 82.1
IPR029413Rhamnogalacturonan lyase, domain IICDDcd10316RGL4_Mcoord: 330..429
e-value: 2.56307E-33
score: 120.817
IPR013784Carbohydrate-binding-like foldSUPERFAMILY49452Starch-binding domain-likecoord: 327..424
IPR011013Galactose mutarotase-like domain superfamilySUPERFAMILY74650Galactose mutarotase-likecoord: 17..290
IPR008979Galactose-binding-like domain superfamilySUPERFAMILY49785Galactose-binding domain-likecoord: 436..627

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008337.1MS008337.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0003824 catalytic activity