MS008323 (gene) Bitter gourd (TR) v1

Overview


Name	MS008323
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ubiquitin-fold modifier-conjugating enzyme 1
Location	scaffold4: 470866 .. 472193 (+)
RNA-Seq Expression	MS008323
Synteny	MS008323

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGTTGGGATCCGAATACCAAGTCGACGCTGACCCAAATCCCTCTGCTCACGACCAAAGCTGGCCCCAGAGATGGCGCGGCGTGGACGCAGCGGCTGAAGGAGGAGTACAAGGCACTAATCGCCTACACCCAGATGAACAAGTCCAATGACAACGACTGGTTCCGGATCTCAGCTGCTAATCCAGAAGGAACACGATGGACCGGCAAGTGCTGGTATATCCACAATCTTCTCAAGTACGAATTCGATCTTCAATTCGATATTCCCGTCACGTATCCCTCTACAGCGCCAGAGCTCGAGCTGCCGGAGCTCGATGGGAAAACCCAGAAGGTTTTAATTTTTTTTTATTTGCTTATATTGAAGGTTTTGCGTTTCTCATGGTTGAATTTGAAAACATTGTTTAATTGGATTGTTTCAGATGTACAGAGGTGGAAAGATCTGCTTAACCATTCATTTCAAGCCTCTATGGGCTAAAAACTGGTAATTTCATCTCTATTCATTTAAATGCCGGTAGAAATAAGAAGATTAGAGTTCACGTTTTTGCACAACTGGCTAGTTTTACTCTGCATTGGTTTGTAAGTGGAAATTAAATCAGAGTCGGCTTTAGGGTTTAGGGTTTTTAATTCATATTCAGAATGCATGCTTGCTTTGATCTGCGTCTCAACAGGCTCGGCTTTTCCAAGTTTTTGACCGTGAATGCCTTATTTCCAAGTTTTCCCTAGGTTATAATCCTCAGTTATTATACACAGAAATCAGATTTGTTCATCATGATTTATGTGAGAGGAACTTTATTTTACATGAATCATGTGATTGCCTTTAGTCAAATCAGTATGACGAGGTTAATTAAGACAACTTCATATTAAGTTTTCTTATCCAGGCCATGGTCAACCACCGAATTCTCAGTTTCGTTCTTATCTATATGATAAGAGTAAGAAGTGAGACATCACATCTAAAAGTTTAGACCATGCAAATTTCTTGCACATCCTAGGATGAGACCTGCACCGAGAAGTGCATTTCGTTTGTGTCATCTACTGTTTTAAATCCATTTGTTTTTTTTAACGACGAAAAATCATCAAATTATGACTGTTTTGGAGCTGTTGTAGGGTATGACACTTTGCGTTTCAGCTTTCCATTGGCATGATTTAGAGCCTACTAAGTACTGTGTAAATAGCCACCTGATAGAGTAGTTGTTTTGCAGTCCTAGGTTTGGAATTGCTCACGCTCTTTGTTTGGGACTTGCACCATGGCTTGCTGCCGAGGTTCCAATTCTCGTCGATTCTGGTATGATCAAGCACAAAGATGATTCAACATCGACCAGTGAATCT

mRNA sequence

ATGGAGGGTTGGGATCCGAATACCAAGTCGACGCTGACCCAAATCCCTCTGCTCACGACCAAAGCTGGCCCCAGAGATGGCGCGGCGTGGACGCAGCGGCTGAAGGAGGAGTACAAGGCACTAATCGCCTACACCCAGATGAACAAGTCCAATGACAACGACTGGTTCCGGATCTCAGCTGCTAATCCAGAAGGAACACGATGGACCGGCAAGTGCTGGTATATCCACAATCTTCTCAAGTACGAATTCGATCTTCAATTCGATATTCCCGTCACGTATCCCTCTACAGCGCCAGAGCTCGAGCTGCCGGAGCTCGATGGGAAAACCCAGAAGATGTACAGAGGTGGAAAGATCTGCTTAACCATTCATTTCAAGCCTCTATGGGCTAAAAACTGTCCTAGGTTTGGAATTGCTCACGCTCTTTGTTTGGGACTTGCACCATGGCTTGCTGCCGAGGTTCCAATTCTCGTCGATTCTGGTATGATCAAGCACAAAGATGATTCAACATCGACCAGTGAATCT

Coding sequence (CDS)

ATGGAGGGTTGGGATCCGAATACCAAGTCGACGCTGACCCAAATCCCTCTGCTCACGACCAAAGCTGGCCCCAGAGATGGCGCGGCGTGGACGCAGCGGCTGAAGGAGGAGTACAAGGCACTAATCGCCTACACCCAGATGAACAAGTCCAATGACAACGACTGGTTCCGGATCTCAGCTGCTAATCCAGAAGGAACACGATGGACCGGCAAGTGCTGGTATATCCACAATCTTCTCAAGTACGAATTCGATCTTCAATTCGATATTCCCGTCACGTATCCCTCTACAGCGCCAGAGCTCGAGCTGCCGGAGCTCGATGGGAAAACCCAGAAGATGTACAGAGGTGGAAAGATCTGCTTAACCATTCATTTCAAGCCTCTATGGGCTAAAAACTGTCCTAGGTTTGGAATTGCTCACGCTCTTTGTTTGGGACTTGCACCATGGCTTGCTGCCGAGGTTCCAATTCTCGTCGATTCTGGTATGATCAAGCACAAAGATGATTCAACATCGACCAGTGAATCT

Protein sequence

MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISAANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICLTIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES

Homology

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BLAST of MS008323 vs. NCBI nr
Match: XP_022142980.1 (ubiquitin-fold modifier-conjugating enzyme 1 [Momordica charantia])

HSP 1 Score: 376.7 bits (966), Expect = 1.1e-100
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES
Sbjct: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 174

[Close]

BLAST of MS008323 vs. NCBI nr
Match: XP_038896748.1 (ubiquitin-fold modifier-conjugating enzyme 1 [Benincasa hispida])

HSP 1 Score: 373.6 bits (958), Expect = 9.1e-100
Identity = 172/174 (98.85%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKD++TSTSES
Sbjct: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDNTTSTSES 174

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BLAST of MS008323 vs. NCBI nr
Match: XP_004136240.1 (ubiquitin-fold modifier-conjugating enzyme 1 [Cucumis sativus] >XP_008466109.1 PREDICTED: ubiquitin-fold modifier-conjugating enzyme 1 isoform X1 [Cucumis melo] >KAA0038640.1 ubiquitin-fold modifier-conjugating enzyme 1 [Cucumis melo var. makuwa] >KGN60292.1 hypothetical protein Csa_002028 [Cucumis sativus] >TYK31241.1 ubiquitin-fold modifier-conjugating enzyme 1 [Cucumis melo var. makuwa])

HSP 1 Score: 373.2 bits (957), Expect = 1.2e-99
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRW GKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWIGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDD+TSTSES
Sbjct: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDTTSTSES 174

[Close]

BLAST of MS008323 vs. NCBI nr
Match: XP_011016957.1 (PREDICTED: ubiquitin-fold modifier-conjugating enzyme 1 [Populus euphratica] >XP_034891859.1 ubiquitin-fold modifier-conjugating enzyme 1 [Populus alba] >KAB5568780.1 hypothetical protein DKX38_002573 [Salix brachista] >KAF9687404.1 hypothetical protein SADUNF_Sadunf02G0090000 [Salix dunnii] >KAF9863727.1 hypothetical protein H0E87_014181 [Populus deltoides] >KAG5251073.1 ubiquitin-fold modifier-conjugating enzyme [Salix suchowensis] >KAG6784362.1 hypothetical protein POTOM_010053 [Populus tomentosa])

HSP 1 Score: 370.9 bits (951), Expect = 5.9e-99
Identity = 168/174 (96.55%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWT RLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTTRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRWTGKCWY+HNLLKYEFDLQFDIPVTYPSTAPELELP+LDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWTGKCWYVHNLLKYEFDLQFDIPVTYPSTAPELELPQLDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           T+HFKPLWAKNCPRFGIAHALCLGLAPWLAAEVP+LVDSGMIKHKDD+TSTSES
Sbjct: 121 TVHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPVLVDSGMIKHKDDATSTSES 174

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BLAST of MS008323 vs. NCBI nr
Match: XP_038718073.1 (ubiquitin-fold modifier-conjugating enzyme 1 [Tripterygium wilfordii] >KAF5739813.1 ubiquitin-fold modifier-conjugating enzyme 1 [Tripterygium wilfordii])

HSP 1 Score: 370.9 bits (951), Expect = 5.9e-99
Identity = 167/174 (95.98%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRWTGKCWY+HNLLKYEFDLQFDIPVTYPSTAPELELP+LDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWTGKCWYVHNLLKYEFDLQFDIPVTYPSTAPELELPQLDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           T+HFKPLWAKNCPRFG+AHALCLGLAPWLAAEVP+LVDSGMIKHKDD+T+TSES
Sbjct: 121 TVHFKPLWAKNCPRFGMAHALCLGLAPWLAAEVPVLVDSGMIKHKDDATTTSES 174

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BLAST of MS008323 vs. ExPASy Swiss-Prot
Match: Q9SXC8 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=At1g27530 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 1.4e-98
Identity = 159/174 (91.38%), Postives = 172/174 (98.85%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLT+IPLLTTKAGPRDGAAWTQRLKEEYK+LIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTRIPLLTTKAGPRDGAAWTQRLKEEYKSLIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           +NPEGTRWTGKCWY+HNLLKYEFDLQFDIP+TYP+TAPELELPE+DGKTQKMYRGGKICL
Sbjct: 61  SNPEGTRWTGKCWYVHNLLKYEFDLQFDIPITYPATAPELELPEIDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           T+HFKPLWAKNCPRFGIAHALCLGLAPWLAAE+PILVDSG IKHKDD+ +++ES
Sbjct: 121 TVHFKPLWAKNCPRFGIAHALCLGLAPWLAAEIPILVDSGAIKHKDDAATSAES 174

[Close]

BLAST of MS008323 vs. ExPASy Swiss-Prot
Match: A2Z5S8 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_33008 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 7.8e-86
Identity = 139/174 (79.89%), Postives = 154/174 (88.51%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWD  TKS + +IPLL+T+AGPRDG AW QRLKEEY+ALIAYT +NKS DNDWFRISA
Sbjct: 1   MEGWDKGTKSVVGEIPLLSTRAGPRDGEAWRQRLKEEYRALIAYTSVNKSKDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRW G CWY+HNL +YEF LQFDIPV YP  APE+ELP LDGKT KMYRGGKICL
Sbjct: 61  ANPEGTRWEGTCWYVHNLRRYEFPLQFDIPVAYPQVAPEIELPTLDGKTHKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           T+HFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGM+KHKDD  + +++
Sbjct: 121 TVHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMVKHKDDEAAPADA 174

[Close]

BLAST of MS008323 vs. ExPASy Swiss-Prot
Match: Q8S625 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0205200 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 7.8e-86
Identity = 139/174 (79.89%), Postives = 154/174 (88.51%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWD  TKS + +IPLL+T+AGPRDG AW QRLKEEY+ALIAYT +NKS DNDWFRISA
Sbjct: 1   MEGWDKGTKSVVGEIPLLSTRAGPRDGEAWRQRLKEEYRALIAYTSVNKSKDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRW G CWY+HNL +YEF LQFDIPV YP  APE+ELP LDGKT KMYRGGKICL
Sbjct: 61  ANPEGTRWEGTCWYVHNLRRYEFPLQFDIPVAYPQVAPEIELPTLDGKTHKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           T+HFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGM+KHKDD  + +++
Sbjct: 121 TVHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMVKHKDDEAAPADA 174

[Close]

BLAST of MS008323 vs. ExPASy Swiss-Prot
Match: Q1ZXC9 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Dictyostelium discoideum OX=44689 GN=ufc1 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 6.4e-72
Identity = 121/163 (74.23%), Postives = 138/163 (84.66%), Query Frame = 0


Query: 5   DPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISAANPE 64
           D +TK T+ QIPLLT KAGPRDG  W  RLKEEY+ALI Y ++NK  DNDWF I  +NP 
Sbjct: 2   DNHTKQTVQQIPLLTVKAGPRDGDKWIDRLKEEYQALIKYVEINKKADNDWFNIE-SNPL 61

Query: 65  GTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICLTIHF 124
           GTRW GKCWYI+N  KYEFDL+FD+PVTYP TAPE+ +PELDGKT+KMYRGGKICLTIHF
Sbjct: 62  GTRWQGKCWYIYNFKKYEFDLEFDMPVTYPETAPEIAIPELDGKTEKMYRGGKICLTIHF 121

Query: 125 KPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDD 168
           KPLW++N P FGIAHAL LGLAPWLAAEVP LVD+G+IKHK+D
Sbjct: 122 KPLWSRNVPHFGIAHALALGLAPWLAAEVPHLVDNGIIKHKED 163

[Close]

BLAST of MS008323 vs. ExPASy Swiss-Prot
Match: A7SM54 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Nematostella vectensis OX=45351 GN=v1g214407 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 2.7e-70
Identity = 118/167 (70.66%), Postives = 134/167 (80.24%), Query Frame = 0


Query: 5   DPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISAANPE 64
           D  TK TL  IPLL TKAGPRDG  W  RLKEEY +LI Y   NK  DNDWFR+  +N E
Sbjct: 3   DEATKKTLAAIPLLKTKAGPRDGKDWVDRLKEEYTSLIKYVSNNKEADNDWFRLE-SNKE 62

Query: 65  GTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICLTIHF 124
           GTRW GKCWYIHNLLKYEFD++FDIP+TYP+TAPE+ LPELDGKT KMYRGGKIC+T HF
Sbjct: 63  GTRWFGKCWYIHNLLKYEFDVEFDIPITYPTTAPEIALPELDGKTAKMYRGGKICMTDHF 122

Query: 125 KPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTST 172
           KPLW +N PRFGIAHA+ LGL PWLA E+P L++ G+IKHKD S S+
Sbjct: 123 KPLWGRNVPRFGIAHAMALGLGPWLAVEIPDLIEKGLIKHKDKSESS 168

[Close]

BLAST of MS008323 vs. ExPASy TrEMBL
Match: A0A6J1CMG1 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Momordica charantia OX=3673 GN=LOC111012975 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 5.2e-101
Identity = 174/174 (100.00%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES
Sbjct: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 174

[Close]

BLAST of MS008323 vs. ExPASy TrEMBL
Match: A0A5D3E6S1 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005300 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.7e-100
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRW GKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWIGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDD+TSTSES
Sbjct: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDTTSTSES 174

[Close]

BLAST of MS008323 vs. ExPASy TrEMBL
Match: A0A0A0LHR4 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Cucumis sativus OX=3659 GN=Csa_3G893420 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.7e-100
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRW GKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWIGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDD+TSTSES
Sbjct: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDTTSTSES 174

[Close]

BLAST of MS008323 vs. ExPASy TrEMBL
Match: A0A1S3CRV9 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Cucumis melo OX=3656 GN=LOC103503631 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 5.7e-100
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRW GKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWIGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDD+TSTSES
Sbjct: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDTTSTSES 174

[Close]

BLAST of MS008323 vs. ExPASy TrEMBL
Match: A0A7J7D0F8 (Ubiquitin-fold modifier-conjugating enzyme 1 OS=Tripterygium wilfordii OX=458696 GN=HS088_TW12G01024 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.9e-99
Identity = 167/174 (95.98%), Postives = 174/174 (100.00%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           ANPEGTRWTGKCWY+HNLLKYEFDLQFDIPVTYPSTAPELELP+LDGKTQKMYRGGKICL
Sbjct: 61  ANPEGTRWTGKCWYVHNLLKYEFDLQFDIPVTYPSTAPELELPQLDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           T+HFKPLWAKNCPRFG+AHALCLGLAPWLAAEVP+LVDSGMIKHKDD+T+TSES
Sbjct: 121 TVHFKPLWAKNCPRFGMAHALCLGLAPWLAAEVPVLVDSGMIKHKDDATTTSES 174

[Close]

BLAST of MS008323 vs. TAIR 10
Match: AT1G27530.1 (CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-fold modifier-conjugating enzyme 1 (InterPro:IPR014806); Has 269 Blast hits to 269 proteins in 110 species: Archae - 0; Bacteria - 0; Metazoa - 175; Fungi - 0; Plants - 42; Viruses - 0; Other Eukaryotes - 52 (source: NCBI BLink). )

HSP 1 Score: 360.1 bits (923), Expect = 9.7e-100
Identity = 159/174 (91.38%), Postives = 172/174 (98.85%), Query Frame = 0


Query: 1   MEGWDPNTKSTLTQIPLLTTKAGPRDGAAWTQRLKEEYKALIAYTQMNKSNDNDWFRISA 60
           MEGWDPNTKSTLT+IPLLTTKAGPRDGAAWTQRLKEEYK+LIAYTQMNKSNDNDWFRISA
Sbjct: 1   MEGWDPNTKSTLTRIPLLTTKAGPRDGAAWTQRLKEEYKSLIAYTQMNKSNDNDWFRISA 60

Query: 61  ANPEGTRWTGKCWYIHNLLKYEFDLQFDIPVTYPSTAPELELPELDGKTQKMYRGGKICL 120
           +NPEGTRWTGKCWY+HNLLKYEFDLQFDIP+TYP+TAPELELPE+DGKTQKMYRGGKICL
Sbjct: 61  SNPEGTRWTGKCWYVHNLLKYEFDLQFDIPITYPATAPELELPEIDGKTQKMYRGGKICL 120

Query: 121 TIHFKPLWAKNCPRFGIAHALCLGLAPWLAAEVPILVDSGMIKHKDDSTSTSES 175
           T+HFKPLWAKNCPRFGIAHALCLGLAPWLAAE+PILVDSG IKHKDD+ +++ES
Sbjct: 121 TVHFKPLWAKNCPRFGIAHALCLGLAPWLAAEIPILVDSGAIKHKDDAATSAES 174

The following BLAST results are available for this feature:

BLAST of MS008323 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142980.1	1.1e-100	100.00	ubiquitin-fold modifier-conjugating enzyme 1 [Momordica charantia]	[more]
XP_038896748.1	9.1e-100	98.85	ubiquitin-fold modifier-conjugating enzyme 1 [Benincasa hispida]	[more]
XP_004136240.1	1.2e-99	98.85	ubiquitin-fold modifier-conjugating enzyme 1 [Cucumis sativus] >XP_008466109.1 P...	[more]
XP_011016957.1	5.9e-99	96.55	PREDICTED: ubiquitin-fold modifier-conjugating enzyme 1 [Populus euphratica] >XP...	[more]
XP_038718073.1	5.9e-99	95.98	ubiquitin-fold modifier-conjugating enzyme 1 [Tripterygium wilfordii] >KAF573981...	[more]

BLAST of MS008323 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXC8	1.4e-98	91.38	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
A2Z5S8	7.8e-86	79.89	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Oryza sativa subsp. indica OX=39...	[more]
Q8S625	7.8e-86	79.89	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Oryza sativa subsp. japonica OX=...	[more]
Q1ZXC9	6.4e-72	74.23	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Dictyostelium discoideum OX=4468...	[more]
A7SM54	2.7e-70	70.66	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Nematostella vectensis OX=45351 ...	[more]

BLAST of MS008323 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMG1	5.2e-101	100.00	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A5D3E6S1	5.7e-100	98.85	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A0A0LHR4	5.7e-100	98.85	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Cucumis sativus OX=3659 GN=Csa_3...	[more]
A0A1S3CRV9	5.7e-100	98.85	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A7J7D0F8	2.9e-99	95.98	Ubiquitin-fold modifier-conjugating enzyme 1 OS=Tripterygium wilfordii OX=458696...	[more]

BLAST of MS008323 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G27530.1	9.7e-100	91.38	CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR0...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 1..170 e-value: 3.5E-93 score: 311.6
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 5..164
IPR014806	Ubiquitin-fold modifier-conjugating enzyme 1	PFAM	PF08694	UFC1	coord: 8..163 e-value: 1.4E-84 score: 281.3
IPR014806	Ubiquitin-fold modifier-conjugating enzyme 1	PIRSF	PIRSF008716	Ufc1	coord: 3..168 e-value: 3.2E-93 score: 307.7
IPR014806	Ubiquitin-fold modifier-conjugating enzyme 1	PANTHER	PTHR12921	UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1	coord: 5..172
None	No IPR available	PIRSR	PIRSR008716-1	PIRSR008716-1	coord: 4..167 e-value: 3.7E-90 score: 297.7
None	No IPR available	PANTHER	PTHR12921:SF0	UBIQUITIN-FOLD MODIFIER-CONJUGATING ENZYME 1	coord: 5..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS008323.1	MS008323.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071569	protein ufmylation
molecular_function	GO:0061657	UFM1 conjugating enzyme activity