MS008287 (gene) Bitter gourd (TR) v1

Overview
NameMS008287
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPeptidase_S9 domain-containing protein
Locationscaffold4: 181048 .. 185251 (-)
RNA-Seq ExpressionMS008287
SyntenyMS008287
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACCTCTCATATCTCACGTGCGACTTCGCCCTTCCCTCACGTGTCTTTGCCTTTGCCGCGCACCAACTTTGCCATGGAACCCGCAAAAATCCGATCCCAATAAACTCTCGTTCCAGGTTGCAGCCAGGGGAAGTTGTCAACGCGAAGCCAGTCAAATGGAAGAAGCTCTTGTTGACGCCGACAAGTTCCGGAGCGAATTCCTCCGGGTTCTGCGTAGTCGACGATCTGGAGAAGGTGCGAAGTTCAATTCGAATTTTATTTTTCTGTTCGCTGTTTTAGTTACCTTTAATGTTTTGATGTCTGGTCTGGGTCAATGAGAAGTTCGAGCATTTTGTTGCAGAAATTCTAGCTTGATTTGAAGGGTCGTCGTCATTTTGCTTAGGCGATGCCAGTTTCTTCCGTTTTACCTCTTTTGAACATCGTTTTGCCTTTTCGTGAGAAATAGGAAGTTTTCATTCTAAGATCTCCCCTTTTTTTTTTCTTTTTTTTTTTTTTTGTCTTTTTTAGGTAGCGGGCATATTGTCTTTTCTTTTTGTGATACTTGGTAGCCAAGACTAACACTGAAGAAAATTCATGTAGTAACAATAAATTACTGTATTTAATTGGCCTACCTACAGAAATTATGAGTTTATGATGTCTATTAATTTGGCGATATTGAATGTGTGATTGAATTTCTCAGTTCCGCTAAGTGTGAAGCCCGCAATGCCTGTCCACCACCCTCTGATTCAGGAGGCCAACCCGCCAACCTTCAGTAAGGTTGAAGTATTACTGGTTCTTTGTTATTTTTTCATCTTTGTTTAAGTAGTAGCCTAAACTATGTTGATTTATTTTACCTGTTTGAACTTTGAAGGCGATGGCATCTTGTCCAAAGGAAAATTTTAGCAATTTGAAGGACTTGCTTCACGAGGAAAATCTTTATCTGACTACGGAGGCAAGCTATGCTCTTATACTTTTCACACTAACTTGTAATTGGGGCATGATTTAACGGTATATTAGGAGTTCAACTAGACCAAAAGTTCTCATCCATGCAAAATATTCAGTAAAATATCTGCTGCCATATTTTAGATAAGAACAATAATTTCTTGGTTGTTAAGCCCGTATGCTAATTGTAAGGAACAAATAATATAGTCTTTTTTTGGATAAAACTAATAACGTAGTTATTGAATAATCAATGTAGGAAGGAGAGCAAGGCCAATTGCCCATATTGATTATTAGTATGAAGGATAGCAGACAGCAAAGAAGACCTGCAATTGTTTTTCTGCACAGTACAAACAAGTGCAAAGAGTGGTTGAGACCGTTGCTTGAGGTAGCAGCTCCTTTTGCTCTTACATTTTGATATTATCACCAAGTTGTTATAACCAGATTATTACAATATACTATTGCCTTTTTCAGGCTTATGCATCGAGGGGATATGTAGCCATTGCCATTGATTCTCGTTACCATGGTGAAAGGGCCAAGGACAAAACCACTTACCGTGATGTATGCCACTTGTAGCTTTAGTTTCTTCTAGCTAGCCAATTGAGTTGTCTTTTATCTGTGAGTTTTTCAGCAATAAAAAGTATGCACTCTTTAACTGGCTTGTCACCCACTGGTGAAACTAGCAAGGTCTTGAGATTTCCCAGAGTCTCAGGCACTGAATTGGTCTATAAGTTATAACTTTGTTGAATCTGGCTGCCACGTAAGTGATAGACCAGTAATTTAGTTTCTTTGGAGGTGTTTAATCTTCCTGAATCGGGAAACTAAACCTGCTATTATGGCATTTATTCCTTTTTTAGATTTGAGATAAACCAGTAATTTAGTAAGTGATAGACCAGTAATATAGTTTCTTTTGAGTAATTTATGCTACTATGGCATTTGGATCCTCTCTAGTCTTTAAACAAAGTGCTCTTTTCTTCTACAAGGTTAAATCCTAACTTAATGTCATAATCTAGTGCAGGCTCTTATATCTTCATGGAAAAGAGGTGACACCATGCCGTTCATATTTGACACGGTAGATCCTCATTCAATTTGGTAACTGAATTAATGCGATGACAAATGAACATTTAGCTGCAAATGTTACTGACAGATTAAGATGGAAAATGGAGAAACTTCAGTTTAATTAATTGGGTGACTTTTGTTAACAAAAAGCTAATAGACTATATTACTTGCAGGCATGGGACTTGATAAAGTTGGCGGATTATCTGACGGAAAGGGAGGATATTGACCCATCTAGAATAGGGATTACTGGTGAATCACTTGGAGGTCAGTCAAAGTAAAACAATATGGACCAAATTAGGACTACCTTTCCTTTGTTATACAAATCTTACCTCTTCAGATTTTCGCTGCAGGAATGCATGCATGGTTTGCTGCTGCTGCTGACACCCGCTATGCTGTGGTTGTCCCCATAATTGGTGTACAGGTAATTAACCCAACTGTTAACACTCCGGATCTTGTGTTAGTAACATTTCTTTCTGGAAAGGAAAAAGGGAGGTCAACTGATACTCGTGCGATATGATTGCAGTGTTTTCGATGGGCCATTGATCATGATAAGTGGCAGGCCCGAGTTGAGAGTATAAAACCCGTTTTTGAAGGTAAGGTGCATGGTAATTGGTAAAGAATTGAACAAGGTGTATGGTTACACACACAAAACAGAAGCTAGGAAGAAGAAAACGTTAAATAATTAAAGACAAAATGAAATAATTAATTATAGTTGATTGATAAGCAAGATCAGATTTAACTGTAACCTCTTGTGTGAGCCTTTTAAAGGTATGATAATTTGAAAATATGAATAACTTTATCAATACACACATGACCTGTCTTTGTATCTTGTCTGCAGAAGCACGAATTGAATTAGGCCTGAGTGAGATCAACAAAGAATTGGTGGAGAAGGTCTGTTCTTTCAAGTTCCTGGTTTAAGGATTGTTGATCTAGACCTAGATAGTGTGCTACTTTTTTATTGCCATTTATCCCAACCTATTTCTATATTGTTCTTATCCCCAAATAGTTGTTCTATATTGTTGGGGGTGTTTATTTGATCCCCCATTTAGTCAAGGGGATTCTGTATCTCCTTGGCCTTTAGGATGTTCTTAATGAAAAGTTAACGTTGGGTGGTGTGTTACAATTTGAAGTTGAATTACCATCATTTATTCTTATATTTTGTTCAGGTCTGGAATAGGATTGCTCCTGGTTTGAGTTCCCAATTTCACTCGATTTATTCAGTTCCAGCTATTGCACCACGCCCTTTGTTGCTATTAAATGGTAATGTTCGTATCCTGCTTATATTATATCATTATAATCACAAACCAACATCAAATCTAGAGAAATTGGAAGATTAAAATATGCAGTTCGAACAGAAAAACTGGTCCTTTATTTATAAGTATCCAGTAGTCCTGACATCATGTCAGTAAACACAAGGTGGTTGTCTCCTTAAAGATAAACTCGCCTGAAAAATAGGTGGATCTCTTAATTTTATAGACACAAGTACGCCATAACCTGCAATTATAACTGCATATGTTTTTCCACTCCTGGTATTTTTGAATTTAAAATCGAATGGTATCTCTGTTCAAATGTATTTAATATGCCATTTGAATTTGGAAACAATAGGTGCAGATGACCCTCGTTGTCCAATTGGAGGTTTGGATGCTCCCGTATCAAGAACACAGACAGCTTATCGGAAGCTTGGTTGCCCAGACAATTTTAAGGTATGTAATATGTATACATGGGTTATCTGGTTTGAATTTAGAATTTCAATACCTAAACATATTATTACGTTTCAGGCTGCAATTTTGCATTTTTATTTTTTAAATTAGAACCTAACTTTTCCACTAGTAAATTTAGCACTTCTAGTTCATATCGGGATGTCTTCTCTCTAATAACAATATTTGCTCTTTCTGTTCACTTGGAGAATCGTGCAAAGCAAAATAAATACAGAGCTTGCTTTACAATTCATCATCATCACTCATTTTGTTACGAAATTATATACATAAATTATCGGACAATATCTCTCCCACTCTTCACTTTGATAGTTGTAGTCTACAATAAGTTTTAAGGGCTTGAAAAAATATTTTTTTTCTTTTATTTTAAAATTAATTTAGAGATTTTTTAAAAGTGAATGCTCATGTTTTACAGTTCATAGCACAACCGGGGATAGGCCACGAAATGACACCAGAGATGGTAAAAGAAGCTAGCGATTGGTTTGACAGGTTTTTAATCCAGAGTAGT

mRNA sequence

ATGGCACCTCTCATATCTCACGTGCGACTTCGCCCTTCCCTCACGTGTCTTTGCCTTTGCCGCGCACCAACTTTGCCATGGAACCCGCAAAAATCCGATCCCAATAAACTCTCGTTCCAGGTTGCAGCCAGGGGAAGTTGTCAACGCGAAGCCAGTCAAATGGAAGAAGCTCTTGTTGACGCCGACAAGTTCCGGAGCGAATTCCTCCGGGTTCTGCGTAGTCGACGATCTGGAGAAGTTCCGCTAAGTGTGAAGCCCGCAATGCCTGTCCACCACCCTCTGATTCAGGAGGCCAACCCGCCAACCTTCAGTAAGGAAGGAGAGCAAGGCCAATTGCCCATATTGATTATTAGTATGAAGGATAGCAGACAGCAAAGAAGACCTGCAATTGTTTTTCTGCACAGTACAAACAAGTGCAAAGAGTGGTTGAGACCGTTGCTTGAGGCTTATGCATCGAGGGGATATGTAGCCATTGCCATTGATTCTCGTTACCATGGTGAAAGGGCCAAGGACAAAACCACTTACCGTGATGCTCTTATATCTTCATGGAAAAGAGGTGACACCATGCCGTTCATATTTGACACGGCATGGGACTTGATAAAGTTGGCGGATTATCTGACGGAAAGGGAGGATATTGACCCATCTAGAATAGGGATTACTGGTGAATCACTTGGAGGAATGCATGCATGGTTTGCTGCTGCTGCTGACACCCGCTATGCTGTGGTTGTCCCCATAATTGGTGTACAGTGTTTTCGATGGGCCATTGATCATGATAAGTGGCAGGCCCGAGTTGAGAGTATAAAACCCGTTTTTGAAGAAGCACGAATTGAATTAGGCCTGAGTGAGATCAACAAAGAATTGGTGGAGAAGGTCTGGAATAGGATTGCTCCTGGTTTGAGTTCCCAATTTCACTCGATTTATTCAGTTCCAGCTATTGCACCACGCCCTTTGTTGCTATTAAATGGTGCAGATGACCCTCGTTGTCCAATTGGAGGTTTGGATGCTCCCGTATCAAGAACACAGACAGCTTATCGGAAGCTTGGTTGCCCAGACAATTTTAAGTTCATAGCACAACCGGGGATAGGCCACGAAATGACACCAGAGATGGTAAAAGAAGCTAGCGATTGGTTTGACAGGTTTTTAATCCAGAGTAGT

Coding sequence (CDS)

ATGGCACCTCTCATATCTCACGTGCGACTTCGCCCTTCCCTCACGTGTCTTTGCCTTTGCCGCGCACCAACTTTGCCATGGAACCCGCAAAAATCCGATCCCAATAAACTCTCGTTCCAGGTTGCAGCCAGGGGAAGTTGTCAACGCGAAGCCAGTCAAATGGAAGAAGCTCTTGTTGACGCCGACAAGTTCCGGAGCGAATTCCTCCGGGTTCTGCGTAGTCGACGATCTGGAGAAGTTCCGCTAAGTGTGAAGCCCGCAATGCCTGTCCACCACCCTCTGATTCAGGAGGCCAACCCGCCAACCTTCAGTAAGGAAGGAGAGCAAGGCCAATTGCCCATATTGATTATTAGTATGAAGGATAGCAGACAGCAAAGAAGACCTGCAATTGTTTTTCTGCACAGTACAAACAAGTGCAAAGAGTGGTTGAGACCGTTGCTTGAGGCTTATGCATCGAGGGGATATGTAGCCATTGCCATTGATTCTCGTTACCATGGTGAAAGGGCCAAGGACAAAACCACTTACCGTGATGCTCTTATATCTTCATGGAAAAGAGGTGACACCATGCCGTTCATATTTGACACGGCATGGGACTTGATAAAGTTGGCGGATTATCTGACGGAAAGGGAGGATATTGACCCATCTAGAATAGGGATTACTGGTGAATCACTTGGAGGAATGCATGCATGGTTTGCTGCTGCTGCTGACACCCGCTATGCTGTGGTTGTCCCCATAATTGGTGTACAGTGTTTTCGATGGGCCATTGATCATGATAAGTGGCAGGCCCGAGTTGAGAGTATAAAACCCGTTTTTGAAGAAGCACGAATTGAATTAGGCCTGAGTGAGATCAACAAAGAATTGGTGGAGAAGGTCTGGAATAGGATTGCTCCTGGTTTGAGTTCCCAATTTCACTCGATTTATTCAGTTCCAGCTATTGCACCACGCCCTTTGTTGCTATTAAATGGTGCAGATGACCCTCGTTGTCCAATTGGAGGTTTGGATGCTCCCGTATCAAGAACACAGACAGCTTATCGGAAGCTTGGTTGCCCAGACAATTTTAAGTTCATAGCACAACCGGGGATAGGCCACGAAATGACACCAGAGATGGTAAAAGAAGCTAGCGATTGGTTTGACAGGTTTTTAATCCAGAGTAGT

Protein sequence

MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVDADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSKEGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASRGYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDIDPSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEEARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGLDAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQSS
Homology
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BLAST of MS008287 vs. NCBI nr
Match: XP_022153900.1 (uncharacterized protein LOC111021276 [Momordica charantia])

HSP 1 Score: 778.1 bits (2008), Expect = 3.5e-221
Identity = 383/412 (92.96%), Postives = 384/412 (93.20%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60
           MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD
Sbjct: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60

Query: 61  ADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK--------------- 120
           ADKFRSEFLRVLRSRRSGE PLSVKPAMPVHHPLIQEANPPTFSK               
Sbjct: 61  ADKFRSEFLRVLRSRRSGEAPLSVKPAMPVHHPLIQEANPPTFSKAMASCPKENFSNLKD 120

Query: 121 ------------EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR 180
                       EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR
Sbjct: 121 LLHEENLYLTTEEGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR 180

Query: 181 GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDID 240
           GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTERED+D
Sbjct: 181 GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDVD 240

Query: 241 PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE 300
           PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE
Sbjct: 241 PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE 300

Query: 301 ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL 360
           ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL
Sbjct: 301 ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL 360

Query: 361 DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQSS 386
           DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQSS
Sbjct: 361 DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQSS 412

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BLAST of MS008287 vs. NCBI nr
Match: XP_038898085.1 (putative esterase YitV [Benincasa hispida])

HSP 1 Score: 660.2 bits (1702), Expect = 1.1e-185
Identity = 328/411 (79.81%), Postives = 355/411 (86.37%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60
           MA L SHV LRPSLT  CLC   T+PWN + S   K S++VAARGS Q+EA+QMEEA+VD
Sbjct: 1   MAILRSHVLLRPSLT--CLCHTATVPWNRRNSYAFKSSYRVAARGSYQKEANQMEEAIVD 60

Query: 61  ADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFS---------------- 120
           ADKFR+EFL VLRSRRSGEVPL+VK A PV +PLIQEANPPTFS                
Sbjct: 61  ADKFRAEFLSVLRSRRSGEVPLNVKHAKPVLNPLIQEANPPTFSEAMASCPKATFSNLKD 120

Query: 121 -----------KEGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR 180
                      +EGEQGQLP+LIISMKDSRQQ+RPAIVFLHSTNKCKEWLRPLLEAYASR
Sbjct: 121 LLHEENLHLTTEEGEQGQLPVLIISMKDSRQQKRPAIVFLHSTNKCKEWLRPLLEAYASR 180

Query: 181 GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDID 240
           GYVAIAIDSRYHGERAK+KTTYRDALISSWKRGDTMPFIFDT WDLIKLADYLT+REDID
Sbjct: 181 GYVAIAIDSRYHGERAKNKTTYRDALISSWKRGDTMPFIFDTVWDLIKLADYLTKREDID 240

Query: 241 PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE 300
           P RIGITGESLGGMHAWFAAAADTRY+VVVPIIGVQCFRWA+D+DKWQARVESIKPVFEE
Sbjct: 241 PCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQCFRWAVDNDKWQARVESIKPVFEE 300

Query: 301 ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL 360
           ARIELG++EINKE+V+KVWNRIAPGL SQF SIYSVPAIAPRPLLLLNGA+DPRCPI GL
Sbjct: 301 ARIELGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNGAEDPRCPIAGL 360

Query: 361 DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQS 385
           DAPVSRTQTAY+K GCP+NFKFI QP IGH+MT EMVKEASDWFDRFL Q+
Sbjct: 361 DAPVSRTQTAYQKFGCPENFKFIPQPEIGHQMTSEMVKEASDWFDRFLCQN 409

[Close]
BLAST of MS008287 vs. NCBI nr
Match: XP_008466042.1 (PREDICTED: putative esterase YitV [Cucumis melo] >KAA0038611.1 putative esterase YitV [Cucumis melo var. makuwa] >TYK31210.1 putative esterase YitV [Cucumis melo var. makuwa])

HSP 1 Score: 642.1 bits (1655), Expect = 3.0e-180
Identity = 318/411 (77.37%), Postives = 347/411 (84.43%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60
           MA LISHVRL PSL   CLC APT PWN Q     K S++VAARG   +E +QMEEA+VD
Sbjct: 1   MAILISHVRLLPSLA--CLCHAPTFPWNRQSLYAFKSSYRVAARGRYPKEVNQMEEAIVD 60

Query: 61  ADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK--------------- 120
           ADKFR+EFLRVLR+RRSGEVPL+VK   PV +PLIQEA+PPTFSK               
Sbjct: 61  ADKFRAEFLRVLRNRRSGEVPLNVKHTEPVLNPLIQEASPPTFSKAMASCPKETSCNLKD 120

Query: 121 ------------EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR 180
                       EGEQGQLPILI+SMK+SRQQ+RP IVFLHSTNKCKEWLRPLLEAYASR
Sbjct: 121 LLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPLLEAYASR 180

Query: 181 GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDID 240
           GYVAIAIDSRYHGERAK KTTYRDALIS+WK+GDTMPFIFDT WDLIKLADYLT REDID
Sbjct: 181 GYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYLTRREDID 240

Query: 241 PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE 300
           P RIGITGESLGGMHAWFAAAADTRY+VVVPIIGVQ F WA+D+DKWQARV+SIKPVFEE
Sbjct: 241 PCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQSFGWAVDNDKWQARVDSIKPVFEE 300

Query: 301 ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL 360
           ARI+LG++EINKE+V+KVWNRIAPGL SQF SIYSVPAIAPRPLLLLNGADDPRCP+ GL
Sbjct: 301 ARIDLGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNGADDPRCPVAGL 360

Query: 361 DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQS 385
           DAPVSR QTAY+K GCP+NFKFIAQ GIGHEMT EMVKEASDWFD+FL +S
Sbjct: 361 DAPVSRIQTAYQKFGCPENFKFIAQTGIGHEMTTEMVKEASDWFDKFLCES 409

[Close]
BLAST of MS008287 vs. NCBI nr
Match: XP_011652622.1 (uncharacterized protein LOC101220970 isoform X1 [Cucumis sativus])

HSP 1 Score: 628.6 bits (1620), Expect = 3.5e-176
Identity = 316/412 (76.70%), Postives = 342/412 (83.01%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPT-LPWNPQKSDPNKLSFQVAARGSCQREASQMEEALV 60
           MA LISHV L P+ +  CL  APT  PWN Q     K  ++VAARG   +E +QMEEA+V
Sbjct: 1   MAILISHVLLLPN-SLACLSHAPTSFPWNRQSFCAFKSLYRVAARGRYPKEVNQMEEAIV 60

Query: 61  DADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK-------------- 120
           DADKFR EFLRVLRSRRSGEVPL+VK   PV +PLIQEANPPTFSK              
Sbjct: 61  DADKFRVEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSKVMASCPKETSCNLK 120

Query: 121 -------------EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYAS 180
                        EGEQGQLPILI+SMK+SRQQ+RP IVFLHSTNKCKEWLRPLLEAYAS
Sbjct: 121 DLLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPLLEAYAS 180

Query: 181 RGYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDI 240
           RGYVAIAIDSRYHGERAK KTTYRDALIS+WK+GDTMPFIFDT WDLIKLADYLT REDI
Sbjct: 181 RGYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYLTRREDI 240

Query: 241 DPSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFE 300
           DP RIGITGESLGGMHAWFAAAADTRY+VVVPIIGVQ F WA+D+DKWQARVESIKPVFE
Sbjct: 241 DPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQSFGWAVDNDKWQARVESIKPVFE 300

Query: 301 EARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGG 360
           EARIELG++EINKE+V+KVWNRIAPGL SQF SIYSVPAIAPRPLLLLNGADDPRCP+ G
Sbjct: 301 EARIELGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNGADDPRCPVAG 360

Query: 361 LDAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQS 385
           LDAPVSR QTAY+K GCP+NFKFI Q GIGHEMT EMVKEASDWFD+FL +S
Sbjct: 361 LDAPVSRIQTAYQKFGCPENFKFITQTGIGHEMTTEMVKEASDWFDKFLCES 411

[Close]
BLAST of MS008287 vs. NCBI nr
Match: XP_022936568.1 (uncharacterized protein LOC111443135 isoform X2 [Cucurbita moschata])

HSP 1 Score: 626.3 bits (1614), Expect = 1.7e-175
Identity = 319/401 (79.55%), Postives = 339/401 (84.54%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60
           MA LI    LRPSLT LCL  A T PWN Q S+  K  ++VAA G+C     QM EA+VD
Sbjct: 1   MAILIPDALLRPSLTRLCL--AATSPWNRQSSNEIKSLYRVAALGNC-----QMGEAVVD 60

Query: 61  ADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK--------------- 120
           ADKFR+EFLRVLRSRRS EVPL+VK  MP     IQE NPP FSK               
Sbjct: 61  ADKFRAEFLRVLRSRRSPEVPLNVKRTMP-----IQETNPPAFSKATFSNLKDLLHEENL 120

Query: 121 -----EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASRGYVAIAI 180
                EGEQG+LPILIISMKDSRQQ+RPAIVFLHSTNKCKEWLRPLLEAYASRGYVAIAI
Sbjct: 121 HLTTEEGEQGKLPILIISMKDSRQQKRPAIVFLHSTNKCKEWLRPLLEAYASRGYVAIAI 180

Query: 181 DSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDIDPSRIGIT 240
           DSRYHGERAK+KTTY DALISSWKRGDTMPFIFDT WDLIKLADYLT+REDIDP RIGIT
Sbjct: 181 DSRYHGERAKNKTTYYDALISSWKRGDTMPFIFDTVWDLIKLADYLTKREDIDPCRIGIT 240

Query: 241 GESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEEARIELGL 300
           GESLGGMHAWFAAAADTRY+VVVPIIGVQCFRWAID+DKWQARVESIKPVFEEARIELG+
Sbjct: 241 GESLGGMHAWFAAAADTRYSVVVPIIGVQCFRWAIDNDKWQARVESIKPVFEEARIELGM 300

Query: 301 SEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGLDAPVSRT 360
           +EI+KE+V+KVWNRIAPGL SQF SIYSVPAIAPRPLLLLNGADDPRCPI GLDAPVSRT
Sbjct: 301 NEIDKEVVKKVWNRIAPGLVSQFGSIYSVPAIAPRPLLLLNGADDPRCPIAGLDAPVSRT 360

Query: 361 QTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFL 382
           Q AY+K GCP+NFKFIAQPGIGHEMTPEMVKEAS WFDRFL
Sbjct: 361 QIAYQKFGCPENFKFIAQPGIGHEMTPEMVKEASQWFDRFL 389

[Close]
BLAST of MS008287 vs. ExPASy Swiss-Prot
Match: O34973 (Putative hydrolase YtaP OS=Bacillus subtilis (strain 168) OX=224308 GN=ytaP PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.8e-11
Identity = 60/232 (25.86%), Postives = 90/232 (38.79%), Query Frame = 0 

Query: 152 SRGYVAIAIDSRYHGER--AKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTER 211
           S GY  +AID    G+R    +   +++ L++       M       +D +   DY+  R
Sbjct: 102 SLGYGVLAIDHWGFGDRRGKAESEIFKEMLLTGKVMWGMM------IYDSLSALDYMQSR 161

Query: 212 EDIDPSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKP 271
            D+ P RIG  G S+GG+ AW+ AA D R  V V +         +DH         IK 
Sbjct: 162 SDVQPDRIGTIGMSMGGLMAWWTAALDDRIKVCVDLCS------QVDHH------VLIK- 221

Query: 272 VFEEARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCP 331
                          + L    +    P L+  F +      IAPRP L L G  D   P
Sbjct: 222 --------------TQNLDRHGFYYYVPSLAKHFSASEIQSLIAPRPHLSLVGVHDRLTP 281

Query: 332 IGGLDAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFL 382
             G+D         Y   G  D ++ + +   GH  T  +  EA  +  ++L
Sbjct: 282 AEGVDKIEKELTAVYAGQGAADCYR-VVRSASGHFETAVIRHEAVRFLQKWL 299

[Close]
BLAST of MS008287 vs. ExPASy Swiss-Prot
Match: A0A1D8EJG8 (4-O-methyl-glucuronoyl methylesterase 1 OS=Sodiomyces alcalophilus OX=398408 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.5e-07
Identity = 53/205 (25.85%), Postives = 90/205 (43.90%), Query Frame = 0 

Query: 196 AWDLIKLADYL--TEREDIDPSRIGITGESLGGMHAWFAAAADTRYAVVVP----IIGVQ 255
           AW + ++ D L  T    IDP+R+G+TG S  G  A  A A + R A+ +P      G  
Sbjct: 279 AWGVARIIDALEKTPAAGIDPTRVGVTGCSRNGKGAMVAGALEPRIALTIPQESGSGGSA 338

Query: 256 CFRWAIDHDKWQARVESIKPVFEEARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSV 315
           C+R +     WQ   +  + V   A+I          + E VW  + P  ++  +++ ++
Sbjct: 339 CWRIS----NWQG--QQGQNVQTPAQI----------ITENVW--LGPVFNNHANNVNAL 398

Query: 316 P--------AIAPRPLLLLNGADDPRCPIGGLDAPVSRTQTAYRKLGCPDNFKFIAQPGI 375
           P         IAPR L ++  +D            ++  +T +  LG  DNF F    G 
Sbjct: 399 PFDHHQLAGLIAPRALYVIENSDMEWLGWTATYGCMAAARTQWEALGALDNFGFSQVGGN 458

Query: 376 GH--EMTPEMVKEASDWFDRFLIQS 385
            H    + +   E + + ++FL+QS
Sbjct: 459 QHCSFNSGKQSAELNAFINKFLLQS 465

[Close]
BLAST of MS008287 vs. ExPASy Swiss-Prot
Match: Q99390 (Uncharacterized 31.7 kDa protein in traX-finO intergenic region OS=Escherichia coli OX=562 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.8e-05
Identity = 36/119 (30.25%), Postives = 53/119 (44.54%), Query Frame = 0 

Query: 126 RRPAIVFLHSTNKCKEWLRP-LLEAYASRGYVAIAIDSRYHGERAKDKTTYRDALISSWK 185
           + P I+  H     +  L P    A+   G+  I  D R  GE             S  +
Sbjct: 24  KHPLIILCHGFCGIRNVLLPCFANAFTEAGFATITFDYRGFGE-------------SDGE 83

Query: 186 RGDTMPFIFDTAWDLIKLADYLTEREDIDPSRIGITGESLGGMHAWFAAAADTRYAVVV 244
           RG  +P +     D+I + ++  ++E ID  RIG+ G SLGG H + AAA D R   +V
Sbjct: 84  RGRLVPAM--QTEDIISVINWAEKQECIDNQRIGLWGTSLGGGHVFSAAAQDQRVKCIV 127

[Close]
BLAST of MS008287 vs. ExPASy Swiss-Prot
Match: B2ABS0 (4-O-methyl-glucuronoyl methylesterase OS=Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383) OX=515849 GN=ge1 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 3.0e-05
Identity = 49/207 (23.67%), Postives = 83/207 (40.10%), Query Frame = 0 

Query: 196 AWDLIKLADYL---TEREDIDPSRIGITGESLGGMHAWFAAAADTRYAVVVP----IIGV 255
           AW + ++ D L     +  IDP+R+G+TG S  G  A  A A + R A+ +P      G 
Sbjct: 263 AWGVSRIVDALELTQAQTGIDPTRLGVTGCSRNGKGAIVAGALEPRIALTLPQESGAGGS 322

Query: 256 QCFRWAIDHDKWQARVESIKPVFEEARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYS 315
            C+R A     WQ                  + +  + + E VW   +P  +S  +++  
Sbjct: 323 GCWRIA----TWQKNNGQ------------NVQDSTQIVQENVW--FSPNFNSYVNNVNQ 382

Query: 316 VP--------AIAPRPLLLLNGADDPRCPIGGLDAPVSRTQTAYRKLGCPDNFKFIAQPG 375
           +P         IAPR L ++   D            +   +  +  LG  DNF +    G
Sbjct: 383 LPFDHHLLAGLIAPRALYVMENVDMEWLGKISTYGCMGIARKQWEALGALDNFGYSQVGG 442

Query: 376 IGHEMTP--EMVKEASDWFDRFLIQSS 386
             H   P  +   E + + ++FL++ S
Sbjct: 443 NSHCSFPSSQQGSELNAFIEKFLLKRS 451

[Close]
BLAST of MS008287 vs. ExPASy Swiss-Prot
Match: P29368 (Uncharacterized 31.7 kDa protein in traX-finO intergenic region OS=Escherichia coli OX=562 PE=4 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 6.7e-05
Identity = 35/119 (29.41%), Postives = 52/119 (43.70%), Query Frame = 0 

Query: 126 RRPAIVFLHSTNKCKEWLRP-LLEAYASRGYVAIAIDSRYHGERAKDKTTYRDALISSWK 185
           + P I+  H     +  L P    A+   G+  I  D R  GE             S  +
Sbjct: 24  KHPLIILCHGFCGIRNVLLPCFANAFTEAGFATITFDYRGFGE-------------SDGE 83

Query: 186 RGDTMPFIFDTAWDLIKLADYLTEREDIDPSRIGITGESLGGMHAWFAAAADTRYAVVV 244
           RG  +P +     D+I + ++  ++E ID  RIG+ G SLGG H + A A D R   +V
Sbjct: 84  RGRLVPAM--QTEDIISVINWAEKQECIDNQRIGLWGTSLGGGHVFSARAQDQRVKCIV 127

[Close]
BLAST of MS008287 vs. ExPASy TrEMBL
Match: A0A6J1DI56 (uncharacterized protein LOC111021276 OS=Momordica charantia OX=3673 GN=LOC111021276 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 1.7e-221
Identity = 383/412 (92.96%), Postives = 384/412 (93.20%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60
           MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD
Sbjct: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60

Query: 61  ADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK--------------- 120
           ADKFRSEFLRVLRSRRSGE PLSVKPAMPVHHPLIQEANPPTFSK               
Sbjct: 61  ADKFRSEFLRVLRSRRSGEAPLSVKPAMPVHHPLIQEANPPTFSKAMASCPKENFSNLKD 120

Query: 121 ------------EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR 180
                       EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR
Sbjct: 121 LLHEENLYLTTEEGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR 180

Query: 181 GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDID 240
           GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTERED+D
Sbjct: 181 GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDVD 240

Query: 241 PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE 300
           PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE
Sbjct: 241 PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE 300

Query: 301 ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL 360
           ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL
Sbjct: 301 ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL 360

Query: 361 DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQSS 386
           DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQSS
Sbjct: 361 DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQSS 412

[Close]
BLAST of MS008287 vs. ExPASy TrEMBL
Match: A0A5A7TAI4 (Putative esterase YitV OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004950 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.5e-180
Identity = 318/411 (77.37%), Postives = 347/411 (84.43%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60
           MA LISHVRL PSL   CLC APT PWN Q     K S++VAARG   +E +QMEEA+VD
Sbjct: 1   MAILISHVRLLPSLA--CLCHAPTFPWNRQSLYAFKSSYRVAARGRYPKEVNQMEEAIVD 60

Query: 61  ADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK--------------- 120
           ADKFR+EFLRVLR+RRSGEVPL+VK   PV +PLIQEA+PPTFSK               
Sbjct: 61  ADKFRAEFLRVLRNRRSGEVPLNVKHTEPVLNPLIQEASPPTFSKAMASCPKETSCNLKD 120

Query: 121 ------------EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR 180
                       EGEQGQLPILI+SMK+SRQQ+RP IVFLHSTNKCKEWLRPLLEAYASR
Sbjct: 121 LLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPLLEAYASR 180

Query: 181 GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDID 240
           GYVAIAIDSRYHGERAK KTTYRDALIS+WK+GDTMPFIFDT WDLIKLADYLT REDID
Sbjct: 181 GYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYLTRREDID 240

Query: 241 PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE 300
           P RIGITGESLGGMHAWFAAAADTRY+VVVPIIGVQ F WA+D+DKWQARV+SIKPVFEE
Sbjct: 241 PCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQSFGWAVDNDKWQARVDSIKPVFEE 300

Query: 301 ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL 360
           ARI+LG++EINKE+V+KVWNRIAPGL SQF SIYSVPAIAPRPLLLLNGADDPRCP+ GL
Sbjct: 301 ARIDLGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNGADDPRCPVAGL 360

Query: 361 DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQS 385
           DAPVSR QTAY+K GCP+NFKFIAQ GIGHEMT EMVKEASDWFD+FL +S
Sbjct: 361 DAPVSRIQTAYQKFGCPENFKFIAQTGIGHEMTTEMVKEASDWFDKFLCES 409

[Close]
BLAST of MS008287 vs. ExPASy TrEMBL
Match: A0A1S3CRR2 (putative esterase YitV OS=Cucumis melo OX=3656 GN=LOC103503593 PE=4 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.5e-180
Identity = 318/411 (77.37%), Postives = 347/411 (84.43%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60
           MA LISHVRL PSL   CLC APT PWN Q     K S++VAARG   +E +QMEEA+VD
Sbjct: 1   MAILISHVRLLPSLA--CLCHAPTFPWNRQSLYAFKSSYRVAARGRYPKEVNQMEEAIVD 60

Query: 61  ADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK--------------- 120
           ADKFR+EFLRVLR+RRSGEVPL+VK   PV +PLIQEA+PPTFSK               
Sbjct: 61  ADKFRAEFLRVLRNRRSGEVPLNVKHTEPVLNPLIQEASPPTFSKAMASCPKETSCNLKD 120

Query: 121 ------------EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASR 180
                       EGEQGQLPILI+SMK+SRQQ+RP IVFLHSTNKCKEWLRPLLEAYASR
Sbjct: 121 LLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPLLEAYASR 180

Query: 181 GYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDID 240
           GYVAIAIDSRYHGERAK KTTYRDALIS+WK+GDTMPFIFDT WDLIKLADYLT REDID
Sbjct: 181 GYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYLTRREDID 240

Query: 241 PSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEE 300
           P RIGITGESLGGMHAWFAAAADTRY+VVVPIIGVQ F WA+D+DKWQARV+SIKPVFEE
Sbjct: 241 PCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQSFGWAVDNDKWQARVDSIKPVFEE 300

Query: 301 ARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGL 360
           ARI+LG++EINKE+V+KVWNRIAPGL SQF SIYSVPAIAPRPLLLLNGADDPRCP+ GL
Sbjct: 301 ARIDLGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNGADDPRCPVAGL 360

Query: 361 DAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQS 385
           DAPVSR QTAY+K GCP+NFKFIAQ GIGHEMT EMVKEASDWFD+FL +S
Sbjct: 361 DAPVSRIQTAYQKFGCPENFKFIAQTGIGHEMTTEMVKEASDWFDKFLCES 409

[Close]
BLAST of MS008287 vs. ExPASy TrEMBL
Match: A0A0A0LJX7 (Peptidase_S9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G895770 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.7e-176
Identity = 316/412 (76.70%), Postives = 342/412 (83.01%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPT-LPWNPQKSDPNKLSFQVAARGSCQREASQMEEALV 60
           MA LISHV L P+ +  CL  APT  PWN Q     K  ++VAARG   +E +QMEEA+V
Sbjct: 1   MAILISHVLLLPN-SLACLSHAPTSFPWNRQSFCAFKSLYRVAARGRYPKEVNQMEEAIV 60

Query: 61  DADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK-------------- 120
           DADKFR EFLRVLRSRRSGEVPL+VK   PV +PLIQEANPPTFSK              
Sbjct: 61  DADKFRVEFLRVLRSRRSGEVPLNVKHTEPVLNPLIQEANPPTFSKVMASCPKETSCNLK 120

Query: 121 -------------EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYAS 180
                        EGEQGQLPILI+SMK+SRQQ+RP IVFLHSTNKCKEWLRPLLEAYAS
Sbjct: 121 DLLHEENLHLTTEEGEQGQLPILIMSMKESRQQKRPGIVFLHSTNKCKEWLRPLLEAYAS 180

Query: 181 RGYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDI 240
           RGYVAIAIDSRYHGERAK KTTYRDALIS+WK+GDTMPFIFDT WDLIKLADYLT REDI
Sbjct: 181 RGYVAIAIDSRYHGERAKTKTTYRDALISAWKKGDTMPFIFDTVWDLIKLADYLTRREDI 240

Query: 241 DPSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFE 300
           DP RIGITGESLGGMHAWFAAAADTRY+VVVPIIGVQ F WA+D+DKWQARVESIKPVFE
Sbjct: 241 DPCRIGITGESLGGMHAWFAAAADTRYSVVVPIIGVQSFGWAVDNDKWQARVESIKPVFE 300

Query: 301 EARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGG 360
           EARIELG++EINKE+V+KVWNRIAPGL SQF SIYSVPAIAPRPLLLLNGADDPRCP+ G
Sbjct: 301 EARIELGMNEINKEVVKKVWNRIAPGLDSQFDSIYSVPAIAPRPLLLLNGADDPRCPVAG 360

Query: 361 LDAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQS 385
           LDAPVSR QTAY+K GCP+NFKFI Q GIGHEMT EMVKEASDWFD+FL +S
Sbjct: 361 LDAPVSRIQTAYQKFGCPENFKFITQTGIGHEMTTEMVKEASDWFDKFLCES 411

[Close]
BLAST of MS008287 vs. ExPASy TrEMBL
Match: A0A6J1FDL4 (uncharacterized protein LOC111443135 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443135 PE=4 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 8.3e-176
Identity = 319/401 (79.55%), Postives = 339/401 (84.54%), Query Frame = 0 

Query: 1   MAPLISHVRLRPSLTCLCLCRAPTLPWNPQKSDPNKLSFQVAARGSCQREASQMEEALVD 60
           MA LI    LRPSLT LCL  A T PWN Q S+  K  ++VAA G+C     QM EA+VD
Sbjct: 1   MAILIPDALLRPSLTRLCL--AATSPWNRQSSNEIKSLYRVAALGNC-----QMGEAVVD 60

Query: 61  ADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPTFSK--------------- 120
           ADKFR+EFLRVLRSRRS EVPL+VK  MP     IQE NPP FSK               
Sbjct: 61  ADKFRAEFLRVLRSRRSPEVPLNVKRTMP-----IQETNPPAFSKATFSNLKDLLHEENL 120

Query: 121 -----EGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLEAYASRGYVAIAI 180
                EGEQG+LPILIISMKDSRQQ+RPAIVFLHSTNKCKEWLRPLLEAYASRGYVAIAI
Sbjct: 121 HLTTEEGEQGKLPILIISMKDSRQQKRPAIVFLHSTNKCKEWLRPLLEAYASRGYVAIAI 180

Query: 181 DSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTEREDIDPSRIGIT 240
           DSRYHGERAK+KTTY DALISSWKRGDTMPFIFDT WDLIKLADYLT+REDIDP RIGIT
Sbjct: 181 DSRYHGERAKNKTTYYDALISSWKRGDTMPFIFDTVWDLIKLADYLTKREDIDPCRIGIT 240

Query: 241 GESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIKPVFEEARIELGL 300
           GESLGGMHAWFAAAADTRY+VVVPIIGVQCFRWAID+DKWQARVESIKPVFEEARIELG+
Sbjct: 241 GESLGGMHAWFAAAADTRYSVVVPIIGVQCFRWAIDNDKWQARVESIKPVFEEARIELGM 300

Query: 301 SEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRCPIGGLDAPVSRT 360
           +EI+KE+V+KVWNRIAPGL SQF SIYSVPAIAPRPLLLLNGADDPRCPI GLDAPVSRT
Sbjct: 301 NEIDKEVVKKVWNRIAPGLVSQFGSIYSVPAIAPRPLLLLNGADDPRCPIAGLDAPVSRT 360

Query: 361 QTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFL 382
           Q AY+K GCP+NFKFIAQPGIGHEMTPEMVKEAS WFDRFL
Sbjct: 361 QIAYQKFGCPENFKFIAQPGIGHEMTPEMVKEASQWFDRFL 389

[Close]
BLAST of MS008287 vs. TAIR 10
Match: AT5G25770.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 458.4 bits (1178), Expect = 5.8e-129
Identity = 218/355 (61.41%), Postives = 270/355 (76.06%), Query Frame = 0 

Query: 54  MEEALVDADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPT----------- 113
           ME  +     FR +FLR+L SRRS +VPL    + P+ +PL Q   P T           
Sbjct: 1   MESPITQPQMFRLQFLRLLHSRRSPQVPLIADSSNPIENPLFQADVPSTTAIESCPKENI 60

Query: 114 --------------FSKEGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLE 173
                          +++ EQG+LP+LI+S+K+  +++RPAIVF+H TN  KEWLRP LE
Sbjct: 61  GKLKDMLKEENIHLHTEDSEQGKLPLLILSLKERSEEKRPAIVFMHGTNTNKEWLRPWLE 120

Query: 174 AYASRGYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTE 233
           AYASRGYVAI +DSRYHGERA  KT YRDALISSW+ G+TMPFIFDT WDLIKLA+YLT+
Sbjct: 121 AYASRGYVAIGLDSRYHGERADCKTAYRDALISSWRNGNTMPFIFDTVWDLIKLAEYLTQ 180

Query: 234 REDIDPSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIK 293
           R+DIDP +IGITG SLGGMHAWFAAAADTRY+VVVP+IGVQ FRWAI++D+W+ARV SIK
Sbjct: 181 RDDIDPKKIGITGISLGGMHAWFAAAADTRYSVVVPLIGVQGFRWAIENDEWEARVNSIK 240

Query: 294 PVFEEARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRC 353
           P+FEEARI+LG + I+KELVEKVWNRIAPGL+S+F S YS+P IAPRPL +LNGA+DPRC
Sbjct: 241 PLFEEARIDLGKNIIDKELVEKVWNRIAPGLASKFDSPYSLPVIAPRPLYILNGANDPRC 300

Query: 354 PIGGLDAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQ 384
           P+GGL+  + R + AY++   P NFKF A+ G+GHE T  M+KE+SDWFD+FL Q
Sbjct: 301 PLGGLELALKRAEKAYKETASPGNFKFKAEDGVGHEATSFMIKESSDWFDKFLKQ 355

[Close]
BLAST of MS008287 vs. TAIR 10
Match: AT5G25770.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 458.4 bits (1178), Expect = 5.8e-129
Identity = 218/355 (61.41%), Postives = 270/355 (76.06%), Query Frame = 0 

Query: 54  MEEALVDADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPT----------- 113
           ME  +     FR +FLR+L SRRS +VPL    + P+ +PL Q   P T           
Sbjct: 59  MESPITQPQMFRLQFLRLLHSRRSPQVPLIADSSNPIENPLFQADVPSTTAIESCPKENI 118

Query: 114 --------------FSKEGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLE 173
                          +++ EQG+LP+LI+S+K+  +++RPAIVF+H TN  KEWLRP LE
Sbjct: 119 GKLKDMLKEENIHLHTEDSEQGKLPLLILSLKERSEEKRPAIVFMHGTNTNKEWLRPWLE 178

Query: 174 AYASRGYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTE 233
           AYASRGYVAI +DSRYHGERA  KT YRDALISSW+ G+TMPFIFDT WDLIKLA+YLT+
Sbjct: 179 AYASRGYVAIGLDSRYHGERADCKTAYRDALISSWRNGNTMPFIFDTVWDLIKLAEYLTQ 238

Query: 234 REDIDPSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIK 293
           R+DIDP +IGITG SLGGMHAWFAAAADTRY+VVVP+IGVQ FRWAI++D+W+ARV SIK
Sbjct: 239 RDDIDPKKIGITGISLGGMHAWFAAAADTRYSVVVPLIGVQGFRWAIENDEWEARVNSIK 298

Query: 294 PVFEEARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRC 353
           P+FEEARI+LG + I+KELVEKVWNRIAPGL+S+F S YS+P IAPRPL +LNGA+DPRC
Sbjct: 299 PLFEEARIDLGKNIIDKELVEKVWNRIAPGLASKFDSPYSLPVIAPRPLYILNGANDPRC 358

Query: 354 PIGGLDAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQ 384
           P+GGL+  + R + AY++   P NFKF A+ G+GHE T  M+KE+SDWFD+FL Q
Sbjct: 359 PLGGLELALKRAEKAYKETASPGNFKFKAEDGVGHEATSFMIKESSDWFDKFLKQ 413

[Close]
BLAST of MS008287 vs. TAIR 10
Match: AT5G25770.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 458.4 bits (1178), Expect = 5.8e-129
Identity = 218/355 (61.41%), Postives = 270/355 (76.06%), Query Frame = 0 

Query: 54  MEEALVDADKFRSEFLRVLRSRRSGEVPLSVKPAMPVHHPLIQEANPPT----------- 113
           ME  +     FR +FLR+L SRRS +VPL    + P+ +PL Q   P T           
Sbjct: 62  MESPITQPQMFRLQFLRLLHSRRSPQVPLIADSSNPIENPLFQADVPSTTAIESCPKENI 121

Query: 114 --------------FSKEGEQGQLPILIISMKDSRQQRRPAIVFLHSTNKCKEWLRPLLE 173
                          +++ EQG+LP+LI+S+K+  +++RPAIVF+H TN  KEWLRP LE
Sbjct: 122 GKLKDMLKEENIHLHTEDSEQGKLPLLILSLKERSEEKRPAIVFMHGTNTNKEWLRPWLE 181

Query: 174 AYASRGYVAIAIDSRYHGERAKDKTTYRDALISSWKRGDTMPFIFDTAWDLIKLADYLTE 233
           AYASRGYVAI +DSRYHGERA  KT YRDALISSW+ G+TMPFIFDT WDLIKLA+YLT+
Sbjct: 182 AYASRGYVAIGLDSRYHGERADCKTAYRDALISSWRNGNTMPFIFDTVWDLIKLAEYLTQ 241

Query: 234 REDIDPSRIGITGESLGGMHAWFAAAADTRYAVVVPIIGVQCFRWAIDHDKWQARVESIK 293
           R+DIDP +IGITG SLGGMHAWFAAAADTRY+VVVP+IGVQ FRWAI++D+W+ARV SIK
Sbjct: 242 RDDIDPKKIGITGISLGGMHAWFAAAADTRYSVVVPLIGVQGFRWAIENDEWEARVNSIK 301

Query: 294 PVFEEARIELGLSEINKELVEKVWNRIAPGLSSQFHSIYSVPAIAPRPLLLLNGADDPRC 353
           P+FEEARI+LG + I+KELVEKVWNRIAPGL+S+F S YS+P IAPRPL +LNGA+DPRC
Sbjct: 302 PLFEEARIDLGKNIIDKELVEKVWNRIAPGLASKFDSPYSLPVIAPRPLYILNGANDPRC 361

Query: 354 PIGGLDAPVSRTQTAYRKLGCPDNFKFIAQPGIGHEMTPEMVKEASDWFDRFLIQ 384
           P+GGL+  + R + AY++   P NFKF A+ G+GHE T  M+KE+SDWFD+FL Q
Sbjct: 362 PLGGLELALKRAEKAYKETASPGNFKFKAEDGVGHEATSFMIKESSDWFDKFLKQ 416

The following BLAST results are available for this feature:
BLAST of MS008287 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022153900.13.5e-22192.96uncharacterized protein LOC111021276 [Momordica charantia][more]
XP_038898085.11.1e-18579.81putative esterase YitV [Benincasa hispida][more]
XP_008466042.13.0e-18077.37PREDICTED: putative esterase YitV [Cucumis melo] >KAA0038611.1 putative esterase... [more]
XP_011652622.13.5e-17676.70uncharacterized protein LOC101220970 isoform X1 [Cucumis sativus][more]
XP_022936568.11.7e-17579.55uncharacterized protein LOC111443135 isoform X2 [Cucurbita moschata][more]
BLAST of MS008287 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O349732.8e-1125.86Putative hydrolase YtaP OS=Bacillus subtilis (strain 168) OX=224308 GN=ytaP PE=3... [more]
A0A1D8EJG82.5e-0725.854-O-methyl-glucuronoyl methylesterase 1 OS=Sodiomyces alcalophilus OX=398408 PE=... [more]
Q993901.8e-0530.25Uncharacterized 31.7 kDa protein in traX-finO intergenic region OS=Escherichia c... [more]
B2ABS03.0e-0523.674-O-methyl-glucuronoyl methylesterase OS=Podospora anserina (strain S / ATCC MYA... [more]
P293686.7e-0529.41Uncharacterized 31.7 kDa protein in traX-finO intergenic region OS=Escherichia c... [more]
BLAST of MS008287 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1DI561.7e-22192.96uncharacterized protein LOC111021276 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A5A7TAI41.5e-18077.37Putative esterase YitV OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4... [more]
A0A1S3CRR21.5e-18077.37putative esterase YitV OS=Cucumis melo OX=3656 GN=LOC103503593 PE=4 SV=1[more]
A0A0A0LJX71.7e-17676.70Peptidase_S9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G89577... [more]
A0A6J1FDL48.3e-17679.55uncharacterized protein LOC111443135 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
BLAST of MS008287 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G25770.15.8e-12961.41alpha/beta-Hydrolases superfamily protein [more]
AT5G25770.25.8e-12961.41alpha/beta-Hydrolases superfamily protein [more]
AT5G25770.35.8e-12961.41alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001375Peptidase S9, prolyl oligopeptidase, catalytic domainPFAMPF00326Peptidase_S9coord: 150..363
e-value: 6.3E-9
score: 35.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 62..385
e-value: 4.6E-50
score: 173.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 68..381
NoneNo IPR availablePANTHERPTHR47381ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 106..381
NoneNo IPR availablePANTHERPTHR47381ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 56..106
NoneNo IPR availablePANTHERPTHR47381:SF2ESTERASE YITV-RELATEDcoord: 56..106
coord: 106..381

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS008287.1MS008287.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008236 serine-type peptidase activity