MS007834 (gene) Bitter gourd (TR) v1

Overview


Name	MS007834
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Chaperone protein dnaJ 20
Location	scaffold13: 1646247 .. 1647626 (+)
RNA-Seq Expression	MS007834
Synteny	MS007834

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTTGCTACGGATTGGCTTTATCCGGCACCGATTCACGTTGCTATCTGCCGGCGATGCCTCCGATCTTCTCTCCGAGAAGAGCCTCTCCGCCGGAGGGAAATTCGCAGGTTTCGTTTCCCAAATCGGTCCGGCATCCCTCGGCGTCTGTCAGAGCTAAGGCCGTCGTCAACGAGGTGCTGGTGTCGGAGGCCGTCGAGCTGAGCTTCTACGAGCTCTTGGGAGTGCCGGAGTCGGGGTCGCTGGTGGAGATTAAGCAGGCGTATAAGCAGATGGCTCGGAAGTATCATCCGGACGTCTCGCCGCCGGACCGGATCGAGGAATATACCAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTGTCCGATCCTGGAACGAGAGCGCTGTATGATAGAGACATGGCCAGAGGTCTTCACCTCGCTTTCTCTGCTCGAAGACGCCACGACAACTGTGAGGTATGTTTTTTAGGCGTTCTGTTTCGGTCGAAAATTGGGGCTGAATTAATTAGGATCTCTTCTCGCCCTTATTTTATTGCAATTTTTTTCGTTTTATTGAATTGATTATTGACCTGTGGGCTTCTGATTCTGTGTACTTGCTAGTAATTATGGATTTTATGTCTAATTTGTTTGACCAATTCGTTCATCCAATTTGGACTACTCCTAGCACTCGATCGCGATTTTCACTTTTTGAACTTTAGCTTTATTTTCATGCTTCTACATGAATTGGCGTTGGGATTGAATTGTAAAAATCCTTTGATTTGATTTTTTCATCCATTTATAGAAACCCAATCTACTTTTCTTTGATTCCGCACAATTTTATGTACGGCAATTAGATGAAGAACATATTCTTAGAACACAATGATGAGTGTTTTTTTTTTAACTCTTTGTTGAACCAAAATTTACAAATTACAAAGGTATACTGTCCATGTACAGATACCAATTTCTGATCCTTTCTTCTATCTCCCTTGTTAAAAATTGTAGCCTTCTTCTACAATAATTTGAAAAATGACAAAATCATACAAGAAAATCATATTTTCAATGACATTTCATGGTTTTCACCTTGTTTATTTCACACATTCCTTACTAAAAATGAAACCCAATCCGGGTTCAAATGACTCAAGTGCACGAAAACAGATAGTACCAAATCACGACTGCTGCACACTGACAGATATTAATCCTTCTAGTTTCTGCATCACAATTTCCTATTTGTAATCCTGTACTTGTTTCTTTCCTCGAACTTCTCAGGACATGGAAGAGAAAAGTGGGTGGAAGAACCGTTGGGAGGATCAGCTGTCAGAATTGAAGAGAAGAAGCATGTACAAGGATTCAAGAGAAAACATGTCATGGGGAGCTCGAATGCGCAGGCAAAAGAAC

mRNA sequence

ATGCGTTGCTACGGATTGGCTTTATCCGGCACCGATTCACGTTGCTATCTGCCGGCGATGCCTCCGATCTTCTCTCCGAGAAGAGCCTCTCCGCCGGAGGGAAATTCGCAGGTTTCGTTTCCCAAATCGGTCCGGCATCCCTCGGCGTCTGTCAGAGCTAAGGCCGTCGTCAACGAGGTGCTGGTGTCGGAGGCCGTCGAGCTGAGCTTCTACGAGCTCTTGGGAGTGCCGGAGTCGGGGTCGCTGGTGGAGATTAAGCAGGCGTATAAGCAGATGGCTCGGAAGTATCATCCGGACGTCTCGCCGCCGGACCGGATCGAGGAATATACCAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTGTCCGATCCTGGAACGAGAGCGCTGTATGATAGAGACATGGCCAGAGGTCTTCACCTCGCTTTCTCTGCTCGAAGACGCCACGACAACTGTGAGGACATGGAAGAGAAAAGTGGGTGGAAGAACCGTTGGGAGGATCAGCTGTCAGAATTGAAGAGAAGAAGCATGTACAAGGATTCAAGAGAAAACATGTCATGGGGAGCTCGAATGCGCAGGCAAAAGAAC

Coding sequence (CDS)

ATGCGTTGCTACGGATTGGCTTTATCCGGCACCGATTCACGTTGCTATCTGCCGGCGATGCCTCCGATCTTCTCTCCGAGAAGAGCCTCTCCGCCGGAGGGAAATTCGCAGGTTTCGTTTCCCAAATCGGTCCGGCATCCCTCGGCGTCTGTCAGAGCTAAGGCCGTCGTCAACGAGGTGCTGGTGTCGGAGGCCGTCGAGCTGAGCTTCTACGAGCTCTTGGGAGTGCCGGAGTCGGGGTCGCTGGTGGAGATTAAGCAGGCGTATAAGCAGATGGCTCGGAAGTATCATCCGGACGTCTCGCCGCCGGACCGGATCGAGGAATATACCAGGAAGTTCATCAAGGTTCAGGAGGCGTACGAGACGTTGTCCGATCCTGGAACGAGAGCGCTGTATGATAGAGACATGGCCAGAGGTCTTCACCTCGCTTTCTCTGCTCGAAGACGCCACGACAACTGTGAGGACATGGAAGAGAAAAGTGGGTGGAAGAACCGTTGGGAGGATCAGCTGTCAGAATTGAAGAGAAGAAGCATGTACAAGGATTCAAGAGAAAACATGTCATGGGGAGCTCGAATGCGCAGGCAAAAGAAC

Protein sequence

MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKAVVNEVLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMYKDSRENMSWGARMRRQKN

Homology

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BLAST of MS007834 vs. NCBI nr
Match: XP_022138808.1 (chaperone protein dnaJ 20, chloroplastic-like [Momordica charantia])

HSP 1 Score: 390.2 bits (1001), Expect = 1.1e-104
Identity = 196/197 (99.49%), Postives = 196/197 (99.49%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKAVVNEV 60
           MRCYGLALSGTDSRCYLPAMPPIFSPRRASP EGNSQVSFPKSVRHPSASVRAKAVVNEV
Sbjct: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPXEGNSQVSFPKSVRHPSASVRAKAVVNEV 60

Query: 61  LVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAY 120
           LVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAY
Sbjct: 61  LVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAY 120

Query: 121 ETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMYK 180
           ETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMYK
Sbjct: 121 ETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMYK 180

Query: 181 DSRENMSWGARMRRQKN 198
           DSRENMSWGARMRRQKN
Sbjct: 181 DSRENMSWGARMRRQKN 197

[Close]

BLAST of MS007834 vs. NCBI nr
Match: XP_023549024.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 325.9 bits (834), Expect = 2.5e-85
Identity = 170/198 (85.86%), Postives = 178/198 (89.90%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKA-VVNE 60
           MR  GLALSGTDSRCYLPAMPPIFSP RA PPEGNSQV FPK  R PSA VRAKA VVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVS+AVE+SFYELLGVPESGS+VEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180
           YETLSDP  R LYDRDMARGLHLAFSARRR  N E+MEEK GWKNRW+DQLSELKRRSM 
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKNRWQDQLSELKRRSMN 180

Query: 181 KDSRENMSWGARMRRQKN 198
           K+SR NMSWGARMRRQ++
Sbjct: 181 KNSRANMSWGARMRRQRD 198

[Close]

BLAST of MS007834 vs. NCBI nr
Match: XP_022959157.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 322.8 bits (826), Expect = 2.1e-84
Identity = 168/198 (84.85%), Postives = 178/198 (89.90%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKA-VVNE 60
           MR  GLALSGTDSRCYLPAMPPIFSP RA PPEGNSQV FPK  R PSA VRAKA VVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVS+AVE+SFYELLGVPESGS+VEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180
           YETLSDP  R LYDRDMARGLHLAFSARRR  N E+M+EK GWK+RW+DQLSELKRRSM 
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSRENMSWGARMRRQKN 198
           K+SR NMSWGARMRRQ++
Sbjct: 181 KNSRANMSWGARMRRQRD 198

[Close]

BLAST of MS007834 vs. NCBI nr
Match: KAG6574846.1 (Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013422.1 Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 320.5 bits (820), Expect = 1.0e-83
Identity = 167/198 (84.34%), Postives = 177/198 (89.39%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKA-VVNE 60
           MR  GLALSGTDSRCYLPAMPPIFSP RA PPEGNSQV FPK  R PSA VRAKA VVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVS+AVE+SFYELLGVPESGS+VEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180
           YETLSDP  R LYDRDMARGLHLAFSARRR  N E+M+EK GWK+RW+DQLSELKRRSM 
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSRENMSWGARMRRQKN 198
           K+SR NMSWGARMR Q++
Sbjct: 181 KNSRANMSWGARMRSQRD 198

[Close]

BLAST of MS007834 vs. NCBI nr
Match: XP_023006292.1 (chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 315.5 bits (807), Expect = 3.3e-82
Identity = 165/196 (84.18%), Postives = 174/196 (88.78%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKA-VVNE 60
           MR  GLALSGTDSRCYLPAMPPIFSP RA P +GNSQV FPK  R PSA VRAKA VV E
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPLDGNSQVLFPKLTRPPSACVRAKATVVKE 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVS+A+E+SFYELLGVPESGS+VEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  TLVSDAIEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180
           YETLSDP  R LYDRDMARGLHLAFSARRR  N E+MEEK GWK+RW+DQLSELKRRSM 
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSRENMSWGARMRRQ 196
           K+SR NMSWGARMRRQ
Sbjct: 181 KNSRANMSWGARMRRQ 196

[Close]

BLAST of MS007834 vs. ExPASy Swiss-Prot
Match: Q9SDN0 (Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ20 PE=1 SV=2)

HSP 1 Score: 174.1 bits (440), Expect = 1.6e-42
Identity = 102/199 (51.26%), Postives = 137/199 (68.84%), Query Frame = 0


Query: 1   MRCY-GLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKAVVNE 60
           M+CY   ++  T+   +     PI S +  S P   + +S+P   R  S  ++++ + ++
Sbjct: 1   MKCYKSSSILSTNHHPFFYKQQPISSLQPTSIP---TTISYPTRTRFSSTRIQSR-LTHD 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
             V ++ +LSFY+LLGV ES +L EIKQAYKQ+ARKYHPDVSPPDR+EEYT +FI+VQEA
Sbjct: 61  DPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARR--RHDNCEDMEEKSGWKNRWEDQLSELKRRS 180
           YETLSDP  R LYDRD++ G   +FS RR  R+D  E +EEKS WK +W+ QLS L+RRS
Sbjct: 121 YETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQ-EVVEEKSEWKAKWQTQLSGLRRRS 180

Query: 181 MYKDSRENMSWGARMRRQK 197
             KD+   MSW ARMRRQ+
Sbjct: 181 NQKDN-NTMSWAARMRRQQ 193

[Close]

BLAST of MS007834 vs. ExPASy Swiss-Prot
Match: Q9WZV3 (Chaperone protein DnaJ OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.9e-12
Identity = 33/65 (50.77%), Postives = 49/65 (75.38%), Query Frame = 0


Query: 70  FYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPGTR 129
           +YE+LGVP   +  EIK+AYK++ +++HPD  P +R +E  ++F ++QEAYE LSDP  R
Sbjct: 8   YYEILGVPRDATQEEIKRAYKRLVKEWHPDRHPENR-KEAEQRFKEIQEAYEVLSDPQKR 67

Query: 130 ALYDR 135
           A+YDR
Sbjct: 68  AMYDR 71

[Close]

BLAST of MS007834 vs. ExPASy Swiss-Prot
Match: A5IIT4 (Chaperone protein DnaJ OS=Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995 / JCM 10881 / RKU-1) OX=390874 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.9e-12
Identity = 33/65 (50.77%), Postives = 49/65 (75.38%), Query Frame = 0


Query: 70  FYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPGTR 129
           +YE+LGVP   +  EIK+AYK++ +++HPD  P +R +E  ++F ++QEAYE LSDP  R
Sbjct: 8   YYEILGVPRDATQEEIKRAYKRLVKEWHPDRHPENR-KEAEQRFKEIQEAYEVLSDPQKR 67

Query: 130 ALYDR 135
           A+YDR
Sbjct: 68  AMYDR 71

[Close]

BLAST of MS007834 vs. ExPASy Swiss-Prot
Match: B1LCI2 (Chaperone protein DnaJ OS=Thermotoga sp. (strain RQ2) OX=126740 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 4.9e-12
Identity = 33/65 (50.77%), Postives = 49/65 (75.38%), Query Frame = 0


Query: 70  FYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPGTR 129
           +YE+LGVP   +  EIK+AYK++ +++HPD  P +R +E  ++F ++QEAYE LSDP  R
Sbjct: 8   YYEILGVPRDATQEEIKRAYKRLVKEWHPDRHPENR-KEAEQRFKEIQEAYEVLSDPQKR 67

Query: 130 ALYDR 135
           A+YDR
Sbjct: 68  AMYDR 71

[Close]

BLAST of MS007834 vs. ExPASy Swiss-Prot
Match: B7IFE0 (Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 6.4e-12
Identity = 35/65 (53.85%), Postives = 48/65 (73.85%), Query Frame = 0


Query: 70  FYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPGTR 129
           +YE+LGV  + S  EI+QAYKQ+ +K+HPD +  +R +E   KF ++QEAYE LSDP  R
Sbjct: 6   YYEILGVSRNASQEEIRQAYKQLIKKWHPDRNHQNR-KEAEEKFKEIQEAYEVLSDPEKR 65

Query: 130 ALYDR 135
           A+YDR
Sbjct: 66  AMYDR 69

[Close]

BLAST of MS007834 vs. ExPASy TrEMBL
Match: A0A6J1CE65 (chaperone protein dnaJ 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111009883 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.1e-105
Identity = 196/197 (99.49%), Postives = 196/197 (99.49%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKAVVNEV 60
           MRCYGLALSGTDSRCYLPAMPPIFSPRRASP EGNSQVSFPKSVRHPSASVRAKAVVNEV
Sbjct: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPXEGNSQVSFPKSVRHPSASVRAKAVVNEV 60

Query: 61  LVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAY 120
           LVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAY
Sbjct: 61  LVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAY 120

Query: 121 ETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMYK 180
           ETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMYK
Sbjct: 121 ETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMYK 180

Query: 181 DSRENMSWGARMRRQKN 198
           DSRENMSWGARMRRQKN
Sbjct: 181 DSRENMSWGARMRRQKN 197

[Close]

BLAST of MS007834 vs. ExPASy TrEMBL
Match: A0A6J1H3S2 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460232 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.0e-84
Identity = 168/198 (84.85%), Postives = 178/198 (89.90%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKA-VVNE 60
           MR  GLALSGTDSRCYLPAMPPIFSP RA PPEGNSQV FPK  R PSA VRAKA VVNE
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPPEGNSQVLFPKLTRPPSACVRAKATVVNE 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVS+AVE+SFYELLGVPESGS+VEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180
           YETLSDP  R LYDRDMARGLHLAFSARRR  N E+M+EK GWK+RW+DQLSELKRRSM 
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMDEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSRENMSWGARMRRQKN 198
           K+SR NMSWGARMRRQ++
Sbjct: 181 KNSRANMSWGARMRRQRD 198

[Close]

BLAST of MS007834 vs. ExPASy TrEMBL
Match: A0A6J1KVG4 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499073 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.6e-82
Identity = 165/196 (84.18%), Postives = 174/196 (88.78%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKA-VVNE 60
           MR  GLALSGTDSRCYLPAMPPIFSP RA P +GNSQV FPK  R PSA VRAKA VV E
Sbjct: 1   MRSCGLALSGTDSRCYLPAMPPIFSPMRACPLDGNSQVLFPKLTRPPSACVRAKATVVKE 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVS+A+E+SFYELLGVPESGS+VEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  TLVSDAIEISFYELLGVPESGSMVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180
           YETLSDP  R LYDRDMARGLHLAFSARRR  N E+MEEK GWK+RW+DQLSELKRRSM 
Sbjct: 121 YETLSDPVMRDLYDRDMARGLHLAFSARRRGYNHEEMEEKGGWKSRWQDQLSELKRRSMN 180

Query: 181 KDSRENMSWGARMRRQ 196
           K+SR NMSWGARMRRQ
Sbjct: 181 KNSRANMSWGARMRRQ 196

[Close]

BLAST of MS007834 vs. ExPASy TrEMBL
Match: A0A6J1KFW2 (chaperone protein dnaJ 20, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111494708 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 6.3e-79
Identity = 159/198 (80.30%), Postives = 175/198 (88.38%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAK-AVVNE 60
           MR YGLALSGTDSRC++PAMPPIFSPRRASP  GNSQV FPKS R+PS  VRAK AVVNE
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPPIFSPRRASPSVGNSQVLFPKSTRNPSLCVRAKAAVVNE 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            LVS+AVE+SFY+LLG+PESGS+ EIKQAYKQMA KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  ALVSDAVEISFYDLLGIPESGSMAEIKQAYKQMALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180
           YETLSDP  RALYDRDM  GLHLAFSARRR +N E+ME KSGWK+ W+DQLS L+RRSM 
Sbjct: 121 YETLSDPRMRALYDRDMLGGLHLAFSARRRGNN-EEMEVKSGWKSLWQDQLSGLRRRSMN 180

Query: 181 KDSRENMSWGARMRRQKN 198
           KDS  +MSWGARMRRQ++
Sbjct: 181 KDSGASMSWGARMRRQRD 197

[Close]

BLAST of MS007834 vs. ExPASy TrEMBL
Match: A0A6J1HHM6 (chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111464407 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 4.5e-77
Identity = 155/198 (78.28%), Postives = 174/198 (87.88%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAK-AVVNE 60
           MR YGLALSGTDSRC++PAMP IFSPRRASP  GNSQV FPKS R PS  VRAK AVV+E
Sbjct: 1   MRGYGLALSGTDSRCFVPAMPSIFSPRRASPSVGNSQVLFPKSTRTPSLCVRAKAAVVDE 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
            +VS+AVE+SFY+LLG+PESGS+ EIKQAYKQ+A KYHPDVSPPDRIEEYTRKFIKVQEA
Sbjct: 61  AVVSDAVEISFYDLLGIPESGSMAEIKQAYKQLALKYHPDVSPPDRIEEYTRKFIKVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRHDNCEDMEEKSGWKNRWEDQLSELKRRSMY 180
           YETLSDP  RALYDRDM  GLHLAFS+RRR +N E+ME KSGWK+RW+DQLS L+RRSM 
Sbjct: 121 YETLSDPSMRALYDRDMLGGLHLAFSSRRRGNN-EEMEVKSGWKSRWQDQLSGLRRRSMN 180

Query: 181 KDSRENMSWGARMRRQKN 198
           KDS  +MSWGARMRRQ++
Sbjct: 181 KDSGASMSWGARMRRQRD 197

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BLAST of MS007834 vs. TAIR 10
Match: AT4G13830.2 (DNAJ-like 20 )

HSP 1 Score: 174.1 bits (440), Expect = 1.1e-43
Identity = 102/199 (51.26%), Postives = 137/199 (68.84%), Query Frame = 0


Query: 1   MRCY-GLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKAVVNE 60
           M+CY   ++  T+   +     PI S +  S P   + +S+P   R  S  ++++ + ++
Sbjct: 1   MKCYKSSSILSTNHHPFFYKQQPISSLQPTSIP---TTISYPTRTRFSSTRIQSR-LTHD 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
             V ++ +LSFY+LLGV ES +L EIKQAYKQ+ARKYHPDVSPPDR+EEYT +FI+VQEA
Sbjct: 61  DPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARR--RHDNCEDMEEKSGWKNRWEDQLSELKRRS 180
           YETLSDP  R LYDRD++ G   +FS RR  R+D  E +EEKS WK +W+ QLS L+RRS
Sbjct: 121 YETLSDPRRRVLYDRDLSMGFSFSFSGRRQNRYDQ-EVVEEKSEWKAKWQTQLSGLRRRS 180

Query: 181 MYKDSRENMSWGARMRRQK 197
             KD+   MSW ARMRRQ+
Sbjct: 181 NQKDN-NTMSWAARMRRQQ 193

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BLAST of MS007834 vs. TAIR 10
Match: AT4G13830.1 (DNAJ-like 20 )

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-32
Identity = 74/151 (49.01%), Postives = 104/151 (68.87%), Query Frame = 0


Query: 1   MRCY-GLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKAVVNE 60
           M+CY   ++  T+   +     PI S +  S P   + +S+P   R  S  ++++ + ++
Sbjct: 1   MKCYKSSSILSTNHHPFFYKQQPISSLQPTSIP---TTISYPTRTRFSSTRIQSR-LTHD 60

Query: 61  VLVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEA 120
             V ++ +LSFY+LLGV ES +L EIKQAYKQ+ARKYHPDVSPPDR+EEYT +FI+VQEA
Sbjct: 61  DPVKQSEDLSFYDLLGVTESVTLPEIKQAYKQLARKYHPDVSPPDRVEEYTDRFIRVQEA 120

Query: 121 YETLSDPGTRALYDRDMARGLHLAFSARRRH 151
           YETLSDP  R LYDRD++ G   +FS RR++
Sbjct: 121 YETLSDPRRRVLYDRDLSMGFSFSFSGRRQN 147

[Close]

BLAST of MS007834 vs. TAIR 10
Match: AT3G13310.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-12
Identity = 46/117 (39.32%), Postives = 64/117 (54.70%), Query Frame = 0


Query: 24  FSPRRASPPEGNSQVSFPKSVRHPSASVRAKAVVN----EVLVSEAVE---LSFYELLGV 83
           FSP     P+  +   +  + R P+ +   KA       E  V+E+V     S YELL V
Sbjct: 12  FSPGNCILPQQRTARFYSGTARFPTGAPSFKASAQTLNAEPAVTESVRRRVSSLYELLKV 71

Query: 84  PESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPGTRALYD 134
            E+ SL EIK AY+ +A+ YHPD S  D      R F+++ +AY TL+DP TRA+YD
Sbjct: 72  NETASLTEIKTAYRSLAKVYHPDASESD-----GRDFMEIHKAYATLADPTTRAIYD 123

[Close]

BLAST of MS007834 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.2e-10
Identity = 46/140 (32.86%), Postives = 64/140 (45.71%), Query Frame = 0


Query: 1   MRCYGLALSGTDSRCYLPAMPPIFSPRRASPPEGNSQVSFPKSVRHPSASVRAKAVVNEV 60
           + C G + S   S   LP +      R   PP   S+            +VRA A     
Sbjct: 42  VNCLGASKSSMFSHGSLPFLSMTGMSRNMHPPRRGSRF-----------TVRADA----- 101

Query: 61  LVSEAVELSFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAY 120
                    +Y +LGV ++ +  EIK AY+++AR YHPDV+     EE   KF ++  AY
Sbjct: 102 --------DYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEE---KFKEISNAY 154

Query: 121 ETLSDPGTRALYDRDMARGL 141
           E LSD   ++LYDR    GL
Sbjct: 162 EVLSDDEKKSLYDRYGEAGL 154

[Close]

BLAST of MS007834 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.1e-10
Identity = 32/72 (44.44%), Postives = 48/72 (66.67%), Query Frame = 0


Query: 69  SFYELLGVPESGSLVEIKQAYKQMARKYHPDVSPPDRIEEYTRKFIKVQEAYETLSDPGT 128
           S+Y++L VP+  S  +IK+AY+++A KYHPD +  +  EE TRKF ++  AYE LSD   
Sbjct: 26  SYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQGN--EEATRKFAEINNAYEVLSDEEK 85

Query: 129 RALYDRDMARGL 141
           R +Y++    GL
Sbjct: 86  REIYNKYGEEGL 95

The following BLAST results are available for this feature:

BLAST of MS007834 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138808.1	1.1e-104	99.49	chaperone protein dnaJ 20, chloroplastic-like [Momordica charantia]	[more]
XP_023549024.1	2.5e-85	85.86	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022959157.1	2.1e-84	84.85	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita moschata]	[more]
KAG6574846.1	1.0e-83	84.34	Chaperone protein dnaJ 20, chloroplastic, partial [Cucurbita argyrosperma subsp....	[more]
XP_023006292.1	3.3e-82	84.18	chaperone protein dnaJ 20, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of MS007834 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SDN0	1.6e-42	51.26	Chaperone protein dnaJ 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ2...	[more]
Q9WZV3	4.9e-12	50.77	Chaperone protein DnaJ OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JC...	[more]
A5IIT4	4.9e-12	50.77	Chaperone protein DnaJ OS=Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995...	[more]
B1LCI2	4.9e-12	50.77	Chaperone protein DnaJ OS=Thermotoga sp. (strain RQ2) OX=126740 GN=dnaJ PE=3 SV=...	[more]
B7IFE0	6.4e-12	53.85	Chaperone protein DnaJ OS=Thermosipho africanus (strain TCF52B) OX=484019 GN=dna...	[more]

BLAST of MS007834 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CE65	5.1e-105	99.49	chaperone protein dnaJ 20, chloroplastic-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1H3S2	1.0e-84	84.85	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1KVG4	1.6e-82	84.18	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1KFW2	6.3e-79	80.30	chaperone protein dnaJ 20, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1HHM6	4.5e-77	78.28	chaperone protein dnaJ 20, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of MS007834 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13830.2	1.1e-43	51.26	DNAJ-like 20	[more]
AT4G13830.1	1.2e-32	49.01	DNAJ-like 20	[more]
AT3G13310.1	1.7e-12	39.32	Chaperone DnaJ-domain superfamily protein	[more]
AT2G22360.1	1.2e-10	32.86	DNAJ heat shock family protein	[more]
AT3G62600.1	2.1e-10	44.44	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 89..104 score: 46.22 coord: 108..128 score: 41.44 coord: 71..89 score: 47.57
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 68..128 e-value: 3.0E-21 score: 86.6
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 70..133 e-value: 3.7E-20 score: 71.8
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 69..136 score: 19.185822
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 70..125 e-value: 1.54225E-19 score: 76.0449
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 59..161 e-value: 2.8E-22 score: 80.7
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 69..139
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 178..197
None	No IPR available	PANTHER	PTHR45090:SF4	CHAPERONE PROTEIN DNAJ 20, CHLOROPLASTIC	coord: 1..197
None	No IPR available	PANTHER	PTHR45090	CHAPERONE PROTEIN DNAJ 20 CHLOROPLASTIC	coord: 1..197
IPR018253	DnaJ domain, conserved site	PROSITE	PS00636	DNAJ_1	coord: 113..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS007834.1	MS007834.1	mRNA