MS007701 (gene) Bitter gourd (TR) v1

BLAST of MS007701 vs. NCBI nr
Match: XP_022138649.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like [Momordica charantia])

HSP 1 Score: 705.3 bits (1819), Expect = 2.7e-199
Identity = 346/358 (96.65%), Postives = 346/358 (96.65%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLHGGEEVVM 60
           RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLHGGEEVVM
Sbjct: 14  RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLHGGEEVVM 73

Query: 61  DQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYFSN 120
           DQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYFSN
Sbjct: 74  DQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYFSN 133

Query: 121 FDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKALR 180
           FDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKAL 
Sbjct: 134 FDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKALG 193

Query: 181 LEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLHHN 240
           LEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLHHN
Sbjct: 194 LEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLHHN 253

Query: 241 RWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVASQ 300
           RWLHVPPLHGALVINLGDFL           QIVSNDAMVSVSHRVAAKKIGPRVSVASQ
Sbjct: 254 RWLHVPPLHGALVINLGDFL-----------QIVSNDAMVSVSHRVAAKKIGPRVSVASQ 313

Query: 301 FRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           FRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL
Sbjct: 314 FRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 360

BLAST of MS007701 vs. NCBI nr
Match: XP_022930403.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 537.3 bits (1383), Expect = 9.8e-149
Identity = 263/360 (73.06%), Postives = 300/360 (83.33%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESELSIPIIDLHGGEEV 60
           RR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE SIPIIDLHG  E 
Sbjct: 12  RRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSIPIIDLHGSVEE 71

Query: 61  VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYF 120
           V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQFYSRDR KKV+YF
Sbjct: 72  VVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFYSRDRTKKVSYF 131

Query: 121 SNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKA 180
           SNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VKKLG  L ELISKA
Sbjct: 132 SNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKLGLTLFELISKA 191

Query: 181 LRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLH 240
           L L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITILLQDQIGGLQV H
Sbjct: 192 LELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILLQDQIGGLQVFH 251

Query: 241 HNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVA 300
            N+W  VPPLHGALVIN GDFLQ          QI+SND MVSVSHRV AK+IGPR S+A
Sbjct: 252 DNQWFDVPPLHGALVINAGDFLQ----------QIISNDKMVSVSHRVVAKEIGPRTSIA 311

Query: 301 SQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           SQFRPY+G+DV NSK+YEPIKELLSEDNPP Y+QV+PKE+I+LH S G D  SKL+LFKL
Sbjct: 312 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 361

BLAST of MS007701 vs. NCBI nr
Match: XP_022930400.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 537.3 bits (1383), Expect = 9.8e-149
Identity = 263/360 (73.06%), Postives = 300/360 (83.33%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESELSIPIIDLHGGEEV 60
           RR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE SIPIIDLHG  E 
Sbjct: 63  RRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSIPIIDLHGSVEE 122

Query: 61  VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYF 120
           V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQFYSRDR KKV+YF
Sbjct: 123 VVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFYSRDRTKKVSYF 182

Query: 121 SNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKA 180
           SNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VKKLG  L ELISKA
Sbjct: 183 SNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKLGLTLFELISKA 242

Query: 181 LRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLH 240
           L L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITILLQDQIGGLQV H
Sbjct: 243 LELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILLQDQIGGLQVFH 302

Query: 241 HNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVA 300
            N+W  VPPLHGALVIN GDFLQ          QI+SND MVSVSHRV AK+IGPR S+A
Sbjct: 303 DNQWFDVPPLHGALVINAGDFLQ----------QIISNDKMVSVSHRVVAKEIGPRTSIA 362

Query: 301 SQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           SQFRPY+G+DV NSK+YEPIKELLSEDNPP Y+QV+PKE+I+LH S G D  SKL+LFKL
Sbjct: 363 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 412

BLAST of MS007701 vs. NCBI nr
Match: XP_022930402.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 537.3 bits (1383), Expect = 9.8e-149
Identity = 263/360 (73.06%), Postives = 300/360 (83.33%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESELSIPIIDLHGGEEV 60
           RR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE SIPIIDLHG  E 
Sbjct: 32  RRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSIPIIDLHGSVEE 91

Query: 61  VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYF 120
           V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQFYSRDR KKV+YF
Sbjct: 92  VVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFYSRDRTKKVSYF 151

Query: 121 SNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKA 180
           SNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VKKLG  L ELISKA
Sbjct: 152 SNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKLGLTLFELISKA 211

Query: 181 LRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLH 240
           L L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITILLQDQIGGLQV H
Sbjct: 212 LELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILLQDQIGGLQVFH 271

Query: 241 HNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVA 300
            N+W  VPPLHGALVIN GDFLQ          QI+SND MVSVSHRV AK+IGPR S+A
Sbjct: 272 DNQWFDVPPLHGALVINAGDFLQ----------QIISNDKMVSVSHRVVAKEIGPRTSIA 331

Query: 301 SQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           SQFRPY+G+DV NSK+YEPIKELLSEDNPP Y+QV+PKE+I+LH S G D  SKL+LFKL
Sbjct: 332 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 381

BLAST of MS007701 vs. NCBI nr
Match: XP_022930401.1 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 535.0 bits (1377), Expect = 4.9e-148
Identity = 262/360 (72.78%), Postives = 299/360 (83.06%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESELSIPIIDLHGGEEV 60
           RR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE SIPIIDLHG  E 
Sbjct: 63  RRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSIPIIDLHGSVEE 122

Query: 61  VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYF 120
           V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQFYSRDR KKV+YF
Sbjct: 123 VVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFYSRDRTKKVSYF 182

Query: 121 SNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKA 180
           SNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VKKLG  L ELISKA
Sbjct: 183 SNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKLGLTLFELISKA 242

Query: 181 LRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLH 240
           L L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITILLQDQIGGLQV H
Sbjct: 243 LELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILLQDQIGGLQVFH 302

Query: 241 HNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVA 300
            N+W  VPPLHGALVIN GDFL           QI+SND MVSVSHRV AK+IGPR S+A
Sbjct: 303 DNQWFDVPPLHGALVINAGDFL-----------QIISNDKMVSVSHRVVAKEIGPRTSIA 362

Query: 301 SQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           SQFRPY+G+DV NSK+YEPIKELLSEDNPP Y+QV+PKE+I+LH S G D  SKL+LFKL
Sbjct: 363 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 411

BLAST of MS007701 vs. ExPASy Swiss-Prot
Match: Q84MB3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At1g06620 PE=2 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 4.8e-106
Identity = 193/367 (52.59%), Postives = 246/367 (67.03%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA---AAESELSIPIIDLHGG-- 60
           R   L++FD +K G KGL DAGIT+IP IF      L +    + S+ SIP IDL GG  
Sbjct: 13  RSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGT 72

Query: 61  ----EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDR 120
                  ++++IG A  KWGFFQV+NHGIP++VL+ MIDGIR FHEQD E KK FYSRD 
Sbjct: 73  DSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDP 132

Query: 121 GKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWIL 180
             K+ Y SNFDLF S AANW+DTL    AP  P P ++P  C E++IEY+KEV KLG +L
Sbjct: 133 ASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLL 192

Query: 181 LELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQI 240
            EL+S+AL L  +HLKD+ C + +L+L HYYPPCP+P LT+G   H+D +F+TILLQD I
Sbjct: 193 FELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHI 252

Query: 241 GGLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKI 300
           GGLQVLH   W+ VPP+ GALV+N+GD L           Q+++ND  +SV HRV A   
Sbjct: 253 GGLQVLHDQYWVDVPPVPGALVVNVGDLL-----------QLITNDKFISVEHRVLANVA 312

Query: 301 GPRVSVASQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSS 359
           GPR+SVA  F  Y+   + N +VY PIKE+LSE+NPP Y+     EY K + SKGFDG+S
Sbjct: 313 GPRISVACFFSSYL---MANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTS 365

BLAST of MS007701 vs. ExPASy Swiss-Prot
Match: O04847 (Deacetoxyvindoline 4-hydroxylase OS=Catharanthus roseus OX=4058 GN=D4H PE=1 SV=2)

HSP 1 Score: 354.4 bits (908), Expect = 1.5e-96
Identity = 177/367 (48.23%), Postives = 251/367 (68.39%), Query Frame = 0

Query: 4   ELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLN----AAAESELSIPIIDLHG---GE 63
           EL++FD +KAG KG+ D GITKIP IF  Q ++L+       +S++ IP+I+L+G     
Sbjct: 44  ELKAFDETKAGVKGIVDTGITKIPRIFIDQPKNLDRISVCRGKSDIKIPVINLNGLSSNS 103

Query: 64  EV---VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDR-G 123
           E+   ++++IG A  K+GFFQ+VNHGIP +V+D M+DG+R FHEQD + K+Q+YSRDR  
Sbjct: 104 EIRREIVEKIGEASEKYGFFQIVNHGIPQDVMDKMVDGVRKFHEQDDQIKRQYYSRDRFN 163

Query: 124 KKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILL 183
           K   Y SN+ L    A NW+DT+   M    PDP E P++CR+I+++Y+  V+ LG IL 
Sbjct: 164 KNFLYSSNYVLIPGIACNWRDTMECIMNSNQPDPQEFPDVCRDILMKYSNYVRNLGLILF 223

Query: 184 ELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIG 243
           EL+S+AL L+ +HL+++ CA+G+++L HYYP CP+P LT GT+ H+D  F+TIL+QDQIG
Sbjct: 224 ELLSEALGLKPNHLEEMDCAEGLILLGHYYPACPQPELTFGTSKHSDSGFLTILMQDQIG 283

Query: 244 GLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIG 303
           GLQ+L  N+W+ VP + GALVIN+ D L           Q+++ND   SV HRV A K+G
Sbjct: 284 GLQILLENQWIDVPFIPGALVINIADLL-----------QLITNDKFKSVEHRVLANKVG 343

Query: 304 PRVSVASQFRPYVGEDVGNS-KVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSS 359
           PR+SVA  F        G S ++Y PIKEL+SE+NPP YK+V  K++I +  +K FD SS
Sbjct: 344 PRISVAVAFGIKTQTQEGVSPRLYGPIKELISEENPPIYKEVTVKDFITIRFAKRFDDSS 399

BLAST of MS007701 vs. ExPASy Swiss-Prot
Match: Q9LTH7 (1-aminocyclopropane-1-carboxylate oxidase homolog 12 OS=Arabidopsis thaliana OX=3702 GN=At5g59540 PE=2 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 8.4e-95
Identity = 176/368 (47.83%), Postives = 242/368 (65.76%), Query Frame = 0

Query: 4   ELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDL-----NAAAESELSIPIIDL------ 63
           E ++FD +K G KGL DA IT++P IFH  +QD+      +A+ S+L IPIID       
Sbjct: 14  ERKAFDETKQGVKGLVDAKITEVPRIFH-HRQDILTNKKPSASVSDLEIPIIDFASVHAD 73

Query: 64  HGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRG 123
               E +++++  A   WGFFQV+NH IP+ VL+ + DG+R FHE+D E KK F+SRD G
Sbjct: 74  TASREAIVEKVKYAVENWGFFQVINHSIPLNVLEEIKDGVRRFHEEDPEVKKSFFSRDAG 133

Query: 124 -KKVAYFSNFDLFQSK-AANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWI 183
            KK  Y SNFDL+ S  + NW+D+ S  +AP  P P EIPE CR+ + EY+K V   G +
Sbjct: 134 NKKFVYNSNFDLYSSSPSVNWRDSFSCYIAPDPPAPEEIPETCRDAMFEYSKHVLSFGGL 193

Query: 184 LLELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQ 243
           L EL+S+AL L+   L+ + C   +LM+ HYYPPCP+P LT+G   H+D +F+T+LLQD 
Sbjct: 194 LFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITKHSDNSFLTLLLQDN 253

Query: 244 IGGLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKK 303
           IGGLQ+LH + W+ V P+HGALV+N+GDFL           Q+++ND  VSV HRV A +
Sbjct: 254 IGGLQILHQDSWVDVSPIHGALVVNIGDFL-----------QLITNDKFVSVEHRVLANR 313

Query: 304 IGPRVSVASQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGS 359
            GPR+SVAS F   +     NS+VY P+KEL+SE+NPPKY+ +  KEY K+   KG DG+
Sbjct: 314 QGPRISVASFFSSSMRP---NSRVYGPMKELVSEENPPKYRDITIKEYSKIFFEKGLDGT 366

BLAST of MS007701 vs. ExPASy Swiss-Prot
Match: Q8H1S4 (1-aminocyclopropane-1-carboxylate oxidase homolog 3 OS=Arabidopsis thaliana OX=3702 GN=At1g06650 PE=2 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 7.2e-94
Identity = 177/372 (47.58%), Postives = 244/372 (65.59%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA--AAESEL----SIPIIDLHG 60
           R +EL++FD +K G KGL D+G++++P IFH     L+      S+L    +IP IDL G
Sbjct: 12  RASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPTIDLGG 71

Query: 61  GE-------EVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY 120
            +          +++I  A  KWGFFQV+NHG+ +E+L+ M  G+R FHEQ  E +K+FY
Sbjct: 72  RDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQEVRKEFY 131

Query: 121 SRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKL 180
           SRD  ++  Y SNFDLF S AANW+DT S +MAP  P P ++PEICR+I++EY+K+V  L
Sbjct: 132 SRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEYSKQVMNL 191

Query: 181 GWILLELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILL 240
           G  L EL+S+AL LE +HL D+ C+ G+LMLSHYYPPCPEP LT+GT+ H+D +F+T+LL
Sbjct: 192 GKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDNSFLTVLL 251

Query: 241 QDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVA 300
            DQI GLQV     W  VP + GAL+IN+GD L           Q+++ND  +S+ HRV 
Sbjct: 252 PDQIEGLQVRREGHWFDVPHVSGALIINIGDLL-----------QLITNDKFISLEHRVL 311

Query: 301 AKK-IGPRVSVASQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKG 359
           A +    RVSVA  F   V     N ++Y PI+EL+SE+NPPKY++   K+Y     +KG
Sbjct: 312 ANRATRARVSVACFFTTGVRP---NPRMYGPIRELVSEENPPKYRETTIKDYATYFNAKG 369

BLAST of MS007701 vs. ExPASy Swiss-Prot
Match: P10967 (1-aminocyclopropane-1-carboxylate oxidase homolog OS=Solanum lycopersicum OX=4081 GN=ACO3 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.9e-93
Identity = 175/368 (47.55%), Postives = 237/368 (64.40%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLHGGEE--- 60
           + +EL++FD++KAG KGL D+GITK+P IF    +D     E+    P+IDL G +E   
Sbjct: 12  KMSELKAFDDTKAGVKGLVDSGITKVPQIFVLPPKDRAKKCETHFVFPVIDLQGIDEDPI 71

Query: 61  ---VVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKK 120
               ++D++  A  KWGFFQVVNHGIP  VLD  + G R F EQD E KKQ+Y+RD  KK
Sbjct: 72  KHKEIVDKVRDASEKWGFFQVVNHGIPTSVLDRTLQGTRQFFEQDNEVKKQYYTRDTAKK 131

Query: 121 VAYFSNFDLFQSK--AANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILL 180
           V Y SN DL++S   AA+W+DT+   MAP  P   E P  C E +I+++K+VKKLG+ LL
Sbjct: 132 VVYTSNLDLYKSSVPAASWRDTIFCYMAPNPPSLQEFPTPCGESLIDFSKDVKKLGFTLL 191

Query: 181 ELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIG 240
           EL+S+ L L+ S+LKD      +    +YYPPCP+P LT+GT  HTD+ F+TILLQD +G
Sbjct: 192 ELLSEGLGLDRSYLKDYMDCFHLFCSCNYYPPCPQPELTMGTIQHTDIGFVTILLQDDMG 251

Query: 241 GLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIG 300
           GLQVLH N W+ VPP  G+LV+N+GDFL           Q++SND  +SV HR  +  +G
Sbjct: 252 GLQVLHQNHWVDVPPTPGSLVVNIGDFL-----------QLLSNDKYLSVEHRAISNNVG 311

Query: 301 PRVSVASQF--RPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGS 359
            R+S+   F   PY      +SK+Y PI ELLSEDNPPKY+    K++     ++G DG+
Sbjct: 312 SRMSITCFFGESPY-----QSSKLYGPITELLSEDNPPKYRATTVKDHTSYLHNRGLDGT 363

BLAST of MS007701 vs. ExPASy TrEMBL
Match: A0A6J1CAP5 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like OS=Momordica charantia OX=3673 GN=LOC111009755 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 346/358 (96.65%), Postives = 346/358 (96.65%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLHGGEEVVM 60
           RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLHGGEEVVM
Sbjct: 14  RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAAAESELSIPIIDLHGGEEVVM 73

Query: 61  DQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYFSN 120
           DQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYFSN
Sbjct: 74  DQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYFSN 133

Query: 121 FDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKALR 180
           FDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKAL 
Sbjct: 134 FDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKALG 193

Query: 181 LEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLHHN 240
           LEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLHHN
Sbjct: 194 LEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLHHN 253

Query: 241 RWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVASQ 300
           RWLHVPPLHGALVINLGDFL           QIVSNDAMVSVSHRVAAKKIGPRVSVASQ
Sbjct: 254 RWLHVPPLHGALVINLGDFL-----------QIVSNDAMVSVSHRVAAKKIGPRVSVASQ 313

Query: 301 FRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           FRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL
Sbjct: 314 FRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 360

BLAST of MS007701 vs. ExPASy TrEMBL
Match: A0A6J1EQD8 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.7e-149
Identity = 263/360 (73.06%), Postives = 300/360 (83.33%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESELSIPIIDLHGGEEV 60
           RR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE SIPIIDLHG  E 
Sbjct: 32  RRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSIPIIDLHGSVEE 91

Query: 61  VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYF 120
           V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQFYSRDR KKV+YF
Sbjct: 92  VVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFYSRDRTKKVSYF 151

Query: 121 SNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKA 180
           SNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VKKLG  L ELISKA
Sbjct: 152 SNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKLGLTLFELISKA 211

Query: 181 LRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLH 240
           L L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITILLQDQIGGLQV H
Sbjct: 212 LELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILLQDQIGGLQVFH 271

Query: 241 HNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVA 300
            N+W  VPPLHGALVIN GDFLQ          QI+SND MVSVSHRV AK+IGPR S+A
Sbjct: 272 DNQWFDVPPLHGALVINAGDFLQ----------QIISNDKMVSVSHRVVAKEIGPRTSIA 331

Query: 301 SQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           SQFRPY+G+DV NSK+YEPIKELLSEDNPP Y+QV+PKE+I+LH S G D  SKL+LFKL
Sbjct: 332 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 381

BLAST of MS007701 vs. ExPASy TrEMBL
Match: A0A6J1EV24 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.7e-149
Identity = 263/360 (73.06%), Postives = 300/360 (83.33%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESELSIPIIDLHGGEEV 60
           RR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE SIPIIDLHG  E 
Sbjct: 12  RRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSIPIIDLHGSVEE 71

Query: 61  VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYF 120
           V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQFYSRDR KKV+YF
Sbjct: 72  VVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFYSRDRTKKVSYF 131

Query: 121 SNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKA 180
           SNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VKKLG  L ELISKA
Sbjct: 132 SNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKLGLTLFELISKA 191

Query: 181 LRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLH 240
           L L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITILLQDQIGGLQV H
Sbjct: 192 LELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILLQDQIGGLQVFH 251

Query: 241 HNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVA 300
            N+W  VPPLHGALVIN GDFLQ          QI+SND MVSVSHRV AK+IGPR S+A
Sbjct: 252 DNQWFDVPPLHGALVINAGDFLQ----------QIISNDKMVSVSHRVVAKEIGPRTSIA 311

Query: 301 SQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           SQFRPY+G+DV NSK+YEPIKELLSEDNPP Y+QV+PKE+I+LH S G D  SKL+LFKL
Sbjct: 312 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 361

BLAST of MS007701 vs. ExPASy TrEMBL
Match: A0A6J1EWT9 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.7e-149
Identity = 263/360 (73.06%), Postives = 300/360 (83.33%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESELSIPIIDLHGGEEV 60
           RR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE SIPIIDLHG  E 
Sbjct: 63  RRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSIPIIDLHGSVEE 122

Query: 61  VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYF 120
           V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQFYSRDR KKV+YF
Sbjct: 123 VVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFYSRDRTKKVSYF 182

Query: 121 SNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKA 180
           SNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VKKLG  L ELISKA
Sbjct: 183 SNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKLGLTLFELISKA 242

Query: 181 LRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLH 240
           L L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITILLQDQIGGLQV H
Sbjct: 243 LELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILLQDQIGGLQVFH 302

Query: 241 HNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVA 300
            N+W  VPPLHGALVIN GDFLQ          QI+SND MVSVSHRV AK+IGPR S+A
Sbjct: 303 DNQWFDVPPLHGALVINAGDFLQ----------QIISNDKMVSVSHRVVAKEIGPRTSIA 362

Query: 301 SQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           SQFRPY+G+DV NSK+YEPIKELLSEDNPP Y+QV+PKE+I+LH S G D  SKL+LFKL
Sbjct: 363 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 412

BLAST of MS007701 vs. ExPASy TrEMBL
Match: A0A6J1EQU3 (1-aminocyclopropane-1-carboxylate oxidase homolog 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436859 PE=3 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 2.3e-148
Identity = 262/360 (72.78%), Postives = 299/360 (83.06%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHC--QKQDLNAAAESELSIPIIDLHGGEEV 60
           RR++LE+F+N+K+G KGL DAGITKIP IFHC  +     AA ESE SIPIIDLHG  E 
Sbjct: 63  RRSQLEAFENTKSGVKGLLDAGITKIPSIFHCHVKPHTKTAATESESSIPIIDLHGSVEE 122

Query: 61  VMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRGKKVAYF 120
           V+D+I +ACRKWGFFQVVNHGIPVEVL+ +I+G+RGFHEQD E KKQFYSRDR KKV+YF
Sbjct: 123 VVDEIRNACRKWGFFQVVNHGIPVEVLEQVINGVRGFHEQDPELKKQFYSRDRTKKVSYF 182

Query: 121 SNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILLELISKA 180
           SNFDLFQSKAANW+DTLS SMAP  P P EIP+ICREIV+EYT++VKKLG  L ELISKA
Sbjct: 183 SNFDLFQSKAANWRDTLSFSMAPSLPLPEEIPQICREIVMEYTEQVKKLGLTLFELISKA 242

Query: 181 LRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIGGLQVLH 240
           L L  +HLKD+GCA+G+LML HYYP CPEP LT+GT NHTDVAFITILLQDQIGGLQV H
Sbjct: 243 LELGDTHLKDMGCAEGLLMLGHYYPACPEPDLTLGTTNHTDVAFITILLQDQIGGLQVFH 302

Query: 241 HNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKIGPRVSVA 300
            N+W  VPPLHGALVIN GDFL           QI+SND MVSVSHRV AK+IGPR S+A
Sbjct: 303 DNQWFDVPPLHGALVINAGDFL-----------QIISNDKMVSVSHRVVAKEIGPRTSIA 362

Query: 301 SQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSSKLELFKL 359
           SQFRPY+G+DV NSK+YEPIKELLSEDNPP Y+QV+PKE+I+LH S G D  SKL+LFKL
Sbjct: 363 SQFRPYMGKDVKNSKLYEPIKELLSEDNPPIYRQVNPKEFIELHFSNGLDECSKLDLFKL 411

BLAST of MS007701 vs. TAIR 10
Match: AT1G06620.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 386.0 bits (990), Expect = 3.4e-107
Identity = 193/367 (52.59%), Postives = 246/367 (67.03%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA---AAESELSIPIIDLHGG-- 60
           R   L++FD +K G KGL DAGIT+IP IF      L +    + S+ SIP IDL GG  
Sbjct: 13  RSTLLKAFDETKTGVKGLIDAGITEIPSIFRAPPATLTSPKPPSSSDFSIPTIDLKGGGT 72

Query: 61  ----EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDR 120
                  ++++IG A  KWGFFQV+NHGIP++VL+ MIDGIR FHEQD E KK FYSRD 
Sbjct: 73  DSITRRSLVEKIGDAAEKWGFFQVINHGIPMDVLEKMIDGIREFHEQDTEVKKGFYSRDP 132

Query: 121 GKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWIL 180
             K+ Y SNFDLF S AANW+DTL    AP  P P ++P  C E++IEY+KEV KLG +L
Sbjct: 133 ASKMVYSSNFDLFSSPAANWRDTLGCYTAPDPPRPEDLPATCGEMMIEYSKEVMKLGKLL 192

Query: 181 LELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQI 240
            EL+S+AL L  +HLKD+ C + +L+L HYYPPCP+P LT+G   H+D +F+TILLQD I
Sbjct: 193 FELLSEALGLNTNHLKDMDCTNSLLLLGHYYPPCPQPDLTLGLTKHSDNSFLTILLQDHI 252

Query: 241 GGLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKKI 300
           GGLQVLH   W+ VPP+ GALV+N+GD L           Q+++ND  +SV HRV A   
Sbjct: 253 GGLQVLHDQYWVDVPPVPGALVVNVGDLL-----------QLITNDKFISVEHRVLANVA 312

Query: 301 GPRVSVASQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSS 359
           GPR+SVA  F  Y+   + N +VY PIKE+LSE+NPP Y+     EY K + SKGFDG+S
Sbjct: 313 GPRISVACFFSSYL---MANPRVYGPIKEILSEENPPNYRDTTITEYAKFYRSKGFDGTS 365

BLAST of MS007701 vs. TAIR 10
Match: AT2G30830.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 362.8 bits (930), Expect = 3.1e-100
Identity = 186/367 (50.68%), Postives = 248/367 (67.57%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDL-NAAAESELSIPIIDLHGG---- 60
           R  E+++FD  K G KGL DAGITK+P IFH Q   + N    S L IP ID+ GG    
Sbjct: 7   RAGEVKAFDQMKIGVKGLVDAGITKVPRIFHHQDVAVTNPKPSSTLEIPTIDVGGGVFES 66

Query: 61  ---EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRG 120
               + V+ ++ +A  K+GFFQV+NHGIP+EV++ M DGIRGFHEQD E KK FYSRD  
Sbjct: 67  TVTRKSVIAKVRAAVEKFGFFQVINHGIPLEVMESMKDGIRGFHEQDSEVKKTFYSRDIT 126

Query: 121 KKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWILL 180
           KKV Y +NFDL+ S+AANW+DTL++ MAP  P   ++P ICREI++EY+K + KLG ++ 
Sbjct: 127 KKVKYNTNFDLYSSQAANWRDTLTMVMAPDVPQAGDLPVICREIMLEYSKRMMKLGELIF 186

Query: 181 ELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQIG 240
           EL+S+AL L+ +HLK++ CA  + +LSHYYPPCPEP  T G ++HTD++FITILLQD IG
Sbjct: 187 ELLSEALGLKPNHLKELNCAKSLSLLSHYYPPCPEPDRTFGISSHTDISFITILLQDHIG 246

Query: 241 GLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKK-I 300
           GLQVLH   W+ VPP   AL++NLGD L           Q+++ND  VSV HRV A +  
Sbjct: 247 GLQVLHDGYWIDVPPNPEALIVNLGDLL-----------QLITNDKFVSVEHRVLANRGE 306

Query: 301 GPRVSVASQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGSS 359
            PR+S AS F       + N +VY P+KELLS+ NPPKY+     E  + + ++G DG+S
Sbjct: 307 EPRISSASFFM----HTIPNEQVYGPMKELLSKQNPPKYRNTTTTEMARHYLARGLDGTS 358

BLAST of MS007701 vs. TAIR 10
Match: AT5G59540.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 348.6 bits (893), Expect = 6.0e-96
Identity = 176/368 (47.83%), Postives = 242/368 (65.76%), Query Frame = 0

Query: 4   ELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDL-----NAAAESELSIPIIDL------ 63
           E ++FD +K G KGL DA IT++P IFH  +QD+      +A+ S+L IPIID       
Sbjct: 14  ERKAFDETKQGVKGLVDAKITEVPRIFH-HRQDILTNKKPSASVSDLEIPIIDFASVHAD 73

Query: 64  HGGEEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYSRDRG 123
               E +++++  A   WGFFQV+NH IP+ VL+ + DG+R FHE+D E KK F+SRD G
Sbjct: 74  TASREAIVEKVKYAVENWGFFQVINHSIPLNVLEEIKDGVRRFHEEDPEVKKSFFSRDAG 133

Query: 124 -KKVAYFSNFDLFQSK-AANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLGWI 183
            KK  Y SNFDL+ S  + NW+D+ S  +AP  P P EIPE CR+ + EY+K V   G +
Sbjct: 134 NKKFVYNSNFDLYSSSPSVNWRDSFSCYIAPDPPAPEEIPETCRDAMFEYSKHVLSFGGL 193

Query: 184 LLELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQDQ 243
           L EL+S+AL L+   L+ + C   +LM+ HYYPPCP+P LT+G   H+D +F+T+LLQD 
Sbjct: 194 LFELLSEALGLKSQTLESMDCVKTLLMICHYYPPCPQPDLTLGITKHSDNSFLTLLLQDN 253

Query: 244 IGGLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAAKK 303
           IGGLQ+LH + W+ V P+HGALV+N+GDFL           Q+++ND  VSV HRV A +
Sbjct: 254 IGGLQILHQDSWVDVSPIHGALVVNIGDFL-----------QLITNDKFVSVEHRVLANR 313

Query: 304 IGPRVSVASQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGFDGS 359
            GPR+SVAS F   +     NS+VY P+KEL+SE+NPPKY+ +  KEY K+   KG DG+
Sbjct: 314 QGPRISVASFFSSSMRP---NSRVYGPMKELVSEENPPKYRDITIKEYSKIFFEKGLDGT 366

BLAST of MS007701 vs. TAIR 10
Match: AT1G06650.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 345.5 bits (885), Expect = 5.1e-95
Identity = 177/372 (47.58%), Postives = 244/372 (65.59%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNA--AAESEL----SIPIIDLHG 60
           R +EL++FD +K G KGL D+G++++P IFH     L+      S+L    +IP IDL G
Sbjct: 12  RASELKAFDETKTGVKGLVDSGVSQVPRIFHHPTVKLSTPKPLPSDLLHLKTIPTIDLGG 71

Query: 61  GE-------EVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFY 120
            +          +++I  A  KWGFFQV+NHG+ +E+L+ M  G+R FHEQ  E +K+FY
Sbjct: 72  RDFQDAIKRNNAIEEIKEAAAKWGFFQVINHGVSLELLEKMKKGVRDFHEQSQEVRKEFY 131

Query: 121 SRDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKL 180
           SRD  ++  Y SNFDLF S AANW+DT S +MAP  P P ++PEICR+I++EY+K+V  L
Sbjct: 132 SRDFSRRFLYLSNFDLFSSPAANWRDTFSCTMAPDTPKPQDLPEICRDIMMEYSKQVMNL 191

Query: 181 GWILLELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILL 240
           G  L EL+S+AL LE +HL D+ C+ G+LMLSHYYPPCPEP LT+GT+ H+D +F+T+LL
Sbjct: 192 GKFLFELLSEALGLEPNHLNDMDCSKGLLMLSHYYPPCPEPDLTLGTSQHSDNSFLTVLL 251

Query: 241 QDQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVA 300
            DQI GLQV     W  VP + GAL+IN+GD L           Q+++ND  +S+ HRV 
Sbjct: 252 PDQIEGLQVRREGHWFDVPHVSGALIINIGDLL-----------QLITNDKFISLEHRVL 311

Query: 301 AKK-IGPRVSVASQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKG 359
           A +    RVSVA  F   V     N ++Y PI+EL+SE+NPPKY++   K+Y     +KG
Sbjct: 312 ANRATRARVSVACFFTTGVRP---NPRMYGPIRELVSEENPPKYRETTIKDYATYFNAKG 369

BLAST of MS007701 vs. TAIR 10
Match: AT2G30840.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 1.9e-94
Identity = 183/371 (49.33%), Postives = 244/371 (65.77%), Query Frame = 0

Query: 1   RRAELESFDNSKAGAKGLSDAGITKIPPIFHCQKQDLNAA----AESELSIPIIDLHGG- 60
           R +E+++FD  K G KGL DAG+T+IP IFH    +L  +    + + + IP IDL GG 
Sbjct: 7   RASEVKAFDELKIGVKGLLDAGVTQIPRIFHHPHLNLTDSNLLLSSTTMVIPTIDLKGGV 66

Query: 61  -------EEVVMDQIGSACRKWGFFQVVNHGIPVEVLDGMIDGIRGFHEQDLEWKKQFYS 120
                   E V+  I  A  ++GFFQV+NHGI  +V++ M DGIRGFHEQD + +K+FY+
Sbjct: 67  FDEYTVTRESVIAMIRDAVERFGFFQVINHGISNDVMEKMKDGIRGFHEQDSDVRKKFYT 126

Query: 121 RDRGKKVAYFSNFDLFQSKAANWKDTLSLSMAPIHPDPHEIPEICREIVIEYTKEVKKLG 180
           RD  K V Y SNFDL+ S +ANW+DTLS  MAP  P+  ++P+IC EI++EY K V KLG
Sbjct: 127 RDVTKTVKYNSNFDLYSSPSANWRDTLSCFMAPDVPETEDLPDICGEIMLEYAKRVMKLG 186

Query: 181 WILLELISKALRLEGSHLKDIGCADGVLMLSHYYPPCPEPHLTIGTANHTDVAFITILLQ 240
            ++ EL+S+AL L  +HLK++ C  G+LMLSHYYPPCPEP LT GT+ H+D +F+TILLQ
Sbjct: 187 ELIFELLSEALGLNPNHLKEMDCTKGLLMLSHYYPPCPEPGLTFGTSPHSDRSFLTILLQ 246

Query: 241 DQIGGLQVLHHNRWLHVPPLHGALVINLGDFLQESAAQFIFKKQIVSNDAMVSVSHRVAA 300
           D IGGLQV  +  W+ VPP+ GAL++NLGD L           Q+++ND  VSV HRV A
Sbjct: 247 DHIGGLQVRQNGYWVDVPPVPGALLVNLGDLL-----------QLMTNDQFVSVEHRVLA 306

Query: 301 KK-IGPRVSVASQFRPYVGEDVGNSKVYEPIKELLSEDNPPKYKQVDPKEYIKLHASKGF 359
            K   PR+SVAS F       + + +VY PIKELLSE N PKY+     EY   + ++G 
Sbjct: 307 NKGEKPRISVASFF----VHPLPSLRVYGPIKELLSEQNLPKYRDTTVTEYTSHYMARGL 362