MS007645 (gene) Bitter gourd (TR) v1

Overview
NameMS007645
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionhomocysteine S-methyltransferase 3-like
Locationscaffold13: 139096 .. 142556 (+)
RNA-Seq ExpressionMS007645
SyntenyMS007645
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAAAGAAACTGCTGAAATGGCTACCTTCATGACCGATTTTCTCCGGCAGTGCGGCGGCTGCGCCGTCGTAGACGGCGGCTTCGCCACTGAACTGGAGCGCCACGGCGCCGATCTCAACGATCCTCTCTGGAGCGCTAAATGCCTCATCAGTTCCCCTCACCTCGTCCGACGGGTACTATTCGAATCCATAATCTCATATAATTTTTCCGATTGGAATTTGATTCTTCGCTTCAGTAGCAGTACATTTCCTGCGGCGTTCCTCGTGAAACAAGAAATTTGCCTTGATTTTGGATTATCTAGGGCATAACTTCAAGAGATTGTTATTTGCTGTCTGAATCTTATTTTTAGAAATAAACTACTATGAAGGAAATTGTTATCTAATCTTTAAAAAGCTTTTTTATTTTTTCCCTAAAGTTTTGCTACTATTTAAAAAAAAAAATGGTAGTAAATTACTTTATCCCTAAAATTGCAGTATCTCCTTCCTCCGTTCATTTCTTAAAATATATATATTATATTAATGATTGACGTATTTTAATAAATTAGAATGGAATTTAGTAATAATTTTTATTAGGCAAGCAACTGTTGTTTCTATTTTTAGTAATGCATTATCCCTTGAAATATGTGCTTCGTTCTGGGCCGGATAACGGATCGGTTGCCGTAGGCCCAGTATGAGGATAGACGGCCCAACCCTTTTCAAATAACAAACAATTTTTTTTAATATTACAAATCAATAATAAGAAAGAAAATGTTATAGAATGAAGAATTTATTAACGTATGTTTGGTTTTGAAGCTAAAAGGATTTTTTTTTTTTTTTGGCAATTCCTTATTTGAAAATTATATTTAAGAAATATTTATTAGTCTATTTTTATTTTGTTTAAAGCTTATCTAGTACTATCGTGCATATGAATAGTTCACAAAACTATGACTCGGTTCATTTTTCTTATCAAAAAGTTTTAGCTTAAATTTTTTATTTGAACATTTTTTTAAATATTACGTGGGTGGAGAGGTACATATGCCTAACTTTTTTTTCTTCTTTTTTTTTTCTCTCTCTTTAGATTACATAGGCCTTAACTTTTGAACATTATTATAGTTGGTAATTGACTGTTACATGTTGATGTGAAACTTACCTCTGTAAAAGGGGAGAAAGAAAAAGCAAATAAAAGTTTTTGCTTTCTTTTGAGCAATCTTCTCAGATTTGCACTATGCCTATCTTTCAAAGCACCACTTTCAAAATATAACAAAGCTTTTAAAGAAAATGTATAGTTTTAGAAAAATTTACTTCAATTCATTTGTTTGATTTTCCGGGTATGCTTTAAAATTGTTTCTCCATCAGCTAATGATCTGCTTTAATATGACAGGTTCATTTAGACTACCTTGATGCTGGGGCCAATATCATTTTGACAGCATCTTATCAGGTTCCCTTAGCATTTTCAGTTTTTCTTTTTGAATCTTTTCTCCACCCTATTTCACTGTTATAATAAAATCATTTTACAGCTTGTAATTGTTAAATTGCCACTCACATGGACTTGCATATTAGTTTACCTCATAACTCTTAAGATTCAACCACTATCACTATTACTTGAGAATTGAGATCTAAAACCTTGAAATTATAAGTTTAGTCCTCGGATTTAAAAAAAATGTCTAATAGATTAGGGATCTATTAAATACTGTCAGGAATCTATGAGACGTCGTCCTAAAAATTTATAGTTTAACCTTGATTTAATCATTTAATATATTCTAACAGTTGTTAGGAATCCATGATTTCAATATGCATGCAGGCTACAATTCAGGGCTTTGAAGCTAAAGGGTTTTCTAGAGTTGAGGGTGAAGAATTGCTAAGAAAAAGTGTTGAAATTGCACTTGAAGCTCGTGAGATCTATCTTGAACAATATACGAAGGATTCATGGGATTTTACTGGAAGTGGAGGGGGTTCGAGGAGGCCAACTCTCGTTGCAGCCTCGGTTGGTAGCTATGGTGCTTATTTGGCTGATGGGTCTGAATACAGGTGAGCAAACTTTTAGCCTTATTTTAGTTTGAGTTTTACTATTTGCAATGTTGGACTTCAATGCTTGTAAGAATTATCCTATCAACTACTTCGCTAGGAAAAAATGATTTCAGCCTAAAGCCTTTGGTCCATTTTCCCTTTTTATATACATATATACCTAATTCTTTTTTTACGGTTGTACCTTGCCTTTTTACAGTGGGAAGTATGGTGATTCAGTTACTCTAGAAATGCTGAAAGATTTTCACAGGAGAAGAGTGCAAATTTTGGCCAACTCAGGTGCTGATTTGATTGCATTTGAGACGATTCCAAACAAGTTGGAAGCTCAGGTATGGAAAACGCTGTTATAAACTTGCAGCATTGGGCCACCATTTATCAACAACCCATTGGCAGGGTTCCAGTTTAGCTTCTTTATGCTGCTTATGTGGAATTTGCATTGCAGGCATATTCTGAGCTTCTTGATGAAGAGGGCATTGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGAATCAATGTGGTTAGTGGGGATTCTATATCTGACTGTGCCTCCATTGCAGATGCTTCCAACCAAGTAGTTGCTGTAGGAATCAACTGTACTCCTCCCAGATATATTCATGGGCTAATCTCGTCGATTCAGAAGGTAAATTCTGATAATACCATCCCACGACTTCTCTTATATAGGAGTTGTAAAATTGTCATAACACATCTTCATCATCTAACCTGTATCAATCAATATATCATGACAAAGAAAATATAGCCAACCTGAGCATGATTGTGATAATTCAATTGGTTAAGGCATACGTGCTCAATTAAAAAGTCAGAGGAGTCTCCATCCCCAAATATCTTTAAACTCAAAGAAAAAAAGATAAAGAGAATATAATGGAGTACAAAACATATCCAAAGAAGTTGAAATAAGATCTGCCCGCAAATGACATCTTTAATAAATTTGAGGGTTGGAACTAGTGTGTGCTGGATGAATTCAATTCAACCTCCCTTCATGGACAATACCAAGATTTGTCACTTAATGAAAATAATATAAGTTAATCTGGTTATCTGATTGGCAGGTCACTAACAAACCAATAGTGATTTATCCAAACAGTGGGGAGACTTATGATGGTGTGACCAAACAGTGGTTGGTGAGTTTTCTGCTCTTTTACGGTCTCATTCCTTCATATCAATGTATACCGAAGAGCAAACAGAGAAACCCGTGAGGGTCGGGTTCGCTCTTCCTCATCCCGCTGCCCTTGTCTTTGTTTTCTGTTTCTCCTCTGAACAATTGTGGTTGCTTCTTTTTTTTGCTGCAGAAATCTGACGGAACGATAGAGGAAGACTTTGTATCATATGTAAGAAAGTGGAAGGAAGCTGGAGCTTCTCTGTTTGGTGGGTGTTGCAGGACCACTCCAAAAACCATCAGAGGCATAGCAAAAGCGCTTTCTTATAAAACTTTT

mRNA sequence

ATGGGTAAAGAAACTGCTGAAATGGCTACCTTCATGACCGATTTTCTCCGGCAGTGCGGCGGCTGCGCCGTCGTAGACGGCGGCTTCGCCACTGAACTGGAGCGCCACGGCGCCGATCTCAACGATCCTCTCTGGAGCGCTAAATGCCTCATCAGTTCCCCTCACCTCGTCCGACGGGTTCATTTAGACTACCTTGATGCTGGGGCCAATATCATTTTGACAGCATCTTATCAGGCTACAATTCAGGGCTTTGAAGCTAAAGGGTTTTCTAGAGTTGAGGGTGAAGAATTGCTAAGAAAAAGTGTTGAAATTGCACTTGAAGCTCGTGAGATCTATCTTGAACAATATACGAAGGATTCATGGGATTTTACTGGAAGTGGAGGGGGTTCGAGGAGGCCAACTCTCGTTGCAGCCTCGGTTGGTAGCTATGGTGCTTATTTGGCTGATGGGTCTGAATACAGTGGGAAGTATGGTGATTCAGTTACTCTAGAAATGCTGAAAGATTTTCACAGGAGAAGAGTGCAAATTTTGGCCAACTCAGGTGCTGATTTGATTGCATTTGAGACGATTCCAAACAAGTTGGAAGCTCAGGCATATTCTGAGCTTCTTGATGAAGAGGGCATTGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGAATCAATGTGGTTAGTGGGGATTCTATATCTGACTGTGCCTCCATTGCAGATGCTTCCAACCAAGTAGTTGCTGTAGGAATCAACTGTACTCCTCCCAGATATATTCATGGGCTAATCTCGTCGATTCAGAAGGTCACTAACAAACCAATAGTGATTTATCCAAACAGTGGGGAGACTTATGATGGTGTGACCAAACAGTGGTTGAAATCTGACGGAACGATAGAGGAAGACTTTGTATCATATGTAAGAAAGTGGAAGGAAGCTGGAGCTTCTCTGTTTGGTGGGTGTTGCAGGACCACTCCAAAAACCATCAGAGGCATAGCAAAAGCGCTTTCTTATAAAACTTTT

Coding sequence (CDS)

ATGGGTAAAGAAACTGCTGAAATGGCTACCTTCATGACCGATTTTCTCCGGCAGTGCGGCGGCTGCGCCGTCGTAGACGGCGGCTTCGCCACTGAACTGGAGCGCCACGGCGCCGATCTCAACGATCCTCTCTGGAGCGCTAAATGCCTCATCAGTTCCCCTCACCTCGTCCGACGGGTTCATTTAGACTACCTTGATGCTGGGGCCAATATCATTTTGACAGCATCTTATCAGGCTACAATTCAGGGCTTTGAAGCTAAAGGGTTTTCTAGAGTTGAGGGTGAAGAATTGCTAAGAAAAAGTGTTGAAATTGCACTTGAAGCTCGTGAGATCTATCTTGAACAATATACGAAGGATTCATGGGATTTTACTGGAAGTGGAGGGGGTTCGAGGAGGCCAACTCTCGTTGCAGCCTCGGTTGGTAGCTATGGTGCTTATTTGGCTGATGGGTCTGAATACAGTGGGAAGTATGGTGATTCAGTTACTCTAGAAATGCTGAAAGATTTTCACAGGAGAAGAGTGCAAATTTTGGCCAACTCAGGTGCTGATTTGATTGCATTTGAGACGATTCCAAACAAGTTGGAAGCTCAGGCATATTCTGAGCTTCTTGATGAAGAGGGCATTGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGAATCAATGTGGTTAGTGGGGATTCTATATCTGACTGTGCCTCCATTGCAGATGCTTCCAACCAAGTAGTTGCTGTAGGAATCAACTGTACTCCTCCCAGATATATTCATGGGCTAATCTCGTCGATTCAGAAGGTCACTAACAAACCAATAGTGATTTATCCAAACAGTGGGGAGACTTATGATGGTGTGACCAAACAGTGGTTGAAATCTGACGGAACGATAGAGGAAGACTTTGTATCATATGTAAGAAAGTGGAAGGAAGCTGGAGCTTCTCTGTTTGGTGGGTGTTGCAGGACCACTCCAAAAACCATCAGAGGCATAGCAAAAGCGCTTTCTTATAAAACTTTT

Protein sequence

MGKETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKWKEAGASLFGGCCRTTPKTIRGIAKALSYKTF
Homology
BLAST of MS007645 vs. NCBI nr
Match: XP_022138564.1 (homocysteine S-methyltransferase 3-like [Momordica charantia])

HSP 1 Score: 674.5 bits (1739), Expect = 4.8e-190
Identity = 334/337 (99.11%), Postives = 334/337 (99.11%), Query Frame = 0

Query: 1   MGKETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRV 60
           MGKETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRV
Sbjct: 1   MGKETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRV 60

Query: 61  HLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDS 120
           HLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIAL AREIYLEQ TKDS
Sbjct: 61  HLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALGAREIYLEQCTKDS 120

Query: 121 WDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANS 180
           WDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANS
Sbjct: 121 WDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANS 180

Query: 181 GADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADAS 240
           GADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADAS
Sbjct: 181 GADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADAS 240

Query: 241 NQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFV 300
           NQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFV
Sbjct: 241 NQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFV 300

Query: 301 SYVRKWKEAGASLFGGCCRTTPKTIRGIAKALSYKTF 338
           SYVRKWKEAGASLFGGCCRTTPKTIRGIAKAL YKTF
Sbjct: 301 SYVRKWKEAGASLFGGCCRTTPKTIRGIAKALFYKTF 337

BLAST of MS007645 vs. NCBI nr
Match: XP_038906597.1 (homocysteine S-methyltransferase 3-like [Benincasa hispida])

HSP 1 Score: 608.6 bits (1568), Expect = 3.3e-170
Identity = 304/337 (90.21%), Postives = 316/337 (93.77%), Query Frame = 0

Query: 1   MGKETAEM-ATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRR 60
           MGKE AEM  TF+TDFLRQCGG  VVDGGFATELER GADLNDPLWSAKCLI+SPHLVRR
Sbjct: 1   MGKEIAEMTTTFLTDFLRQCGGYGVVDGGFATELERLGADLNDPLWSAKCLITSPHLVRR 60

Query: 61  VHLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKD 120
           VHLDYLDAGANII TASYQAT+QGFEAKGFSR E E+LLRKSVEIALEAR+I LE+ TKD
Sbjct: 61  VHLDYLDAGANIISTASYQATLQGFEAKGFSRDESEDLLRKSVEIALEARDICLERCTKD 120

Query: 121 SWDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILAN 180
           SWDFT SG GSRRP LVAASVGSYGAYLADGSEYSG+YGDSVTLE LKDFHRRRVQILAN
Sbjct: 121 SWDFTTSGEGSRRPILVAASVGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILAN 180

Query: 181 SGADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA 240
           +GADLIAFETIPNKLEAQAY+ELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA
Sbjct: 181 AGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA 240

Query: 241 SNQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDF 300
           S QVVAVGINCTPPRYIHGLISSI++VTNKPIV+YPNSGETYDG TKQWLKSDG I EDF
Sbjct: 241 SKQVVAVGINCTPPRYIHGLISSIREVTNKPIVVYPNSGETYDGATKQWLKSDGMIGEDF 300

Query: 301 VSYVRKWKEAGASLFGGCCRTTPKTIRGIAKALSYKT 337
           VSYVRKWKEAGASLFGGCCRTTP TIRGIAKALS KT
Sbjct: 301 VSYVRKWKEAGASLFGGCCRTTPNTIRGIAKALSSKT 337

BLAST of MS007645 vs. NCBI nr
Match: XP_023549248.1 (homocysteine S-methyltransferase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 596.3 bits (1536), Expect = 1.7e-166
Identity = 294/332 (88.55%), Postives = 308/332 (92.77%), Query Frame = 0

Query: 6   AEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYL 65
           AE  TFMTDFLRQCGG  V+DGG ATELERHGADLNDPLWSAKCL+SSPH+VRRVHLDYL
Sbjct: 8   AETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYL 67

Query: 66  DAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTG 125
           DAGANII TASYQATIQGFEAKGFSR E EELLRKSVEIALEAREIYLE+ TKDSWD+T 
Sbjct: 68  DAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTA 127

Query: 126 SGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLI 185
           +  GS RP LVAASVGSYGAYLADGSEYSGKYGDSV+LE LKDFHRRRVQILAN+GADLI
Sbjct: 128 TERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLI 187

Query: 186 AFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVA 245
           AFETIPNKLEAQAY+ELLDEEGIEIPAWFSFNSKDGINVVSGDSI+DCASIADAS +VVA
Sbjct: 188 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVA 247

Query: 246 VGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRK 305
           VGINCTPPRYIHGLISSI++VTNKPIVIYPNSGETYDG TKQWLKSDG   EDFVSYV K
Sbjct: 248 VGINCTPPRYIHGLISSIREVTNKPIVIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSK 307

Query: 306 WKEAGASLFGGCCRTTPKTIRGIAKALSYKTF 338
           WKEAGASLFGGCCRTTP TI+GIAKALS K F
Sbjct: 308 WKEAGASLFGGCCRTTPNTIQGIAKALSSKAF 339

BLAST of MS007645 vs. NCBI nr
Match: KAG7013538.1 (Homocysteine S-methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 595.1 bits (1533), Expect = 3.7e-166
Identity = 293/332 (88.25%), Postives = 308/332 (92.77%), Query Frame = 0

Query: 6   AEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYL 65
           AE  TFMTDFLRQCGG  V+DGG ATELERHGADLNDPLWSAKCL+SSPH+VRRVHLDYL
Sbjct: 21  AETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYL 80

Query: 66  DAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTG 125
           DAGANII TASYQATIQGFEAKGFSR E EELLRKSVEIALEAREIYLE+ TKDSWD+T 
Sbjct: 81  DAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTA 140

Query: 126 SGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLI 185
           S  GS RP LVAASVGSYGAYLADGSEYSGKYGDSV+LE LKDFHRRRVQILAN+GADLI
Sbjct: 141 SERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLI 200

Query: 186 AFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVA 245
           AFETIPNKLEAQAY+ELLDEEGIEIPAWFSFNSKDGINVVSGDSI+DCASIADAS +VVA
Sbjct: 201 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSITDCASIADASKRVVA 260

Query: 246 VGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRK 305
           VGINCTPPRYIHGLISSI++VTNKPI+IYPNSGETYDG TKQWLKSDG   EDFVSYV K
Sbjct: 261 VGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSK 320

Query: 306 WKEAGASLFGGCCRTTPKTIRGIAKALSYKTF 338
           WKEAGASLFGGCCRTTP TI+GIAK+LS K F
Sbjct: 321 WKEAGASLFGGCCRTTPNTIQGIAKSLSSKAF 352

BLAST of MS007645 vs. NCBI nr
Match: XP_008449335.1 (PREDICTED: homocysteine S-methyltransferase 3 [Cucumis melo])

HSP 1 Score: 594.3 bits (1531), Expect = 6.3e-166
Identity = 291/338 (86.09%), Postives = 316/338 (93.49%), Query Frame = 0

Query: 1   MGKETAEMA-TFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRR 60
           MGK+  E A T++T FL++CGG  V+DGGFATELER GADLNDPLWSAKCL++SPHLVRR
Sbjct: 1   MGKQMPETATTYLTHFLQKCGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRR 60

Query: 61  VHLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKD 120
           VHLDYLDAGANII TASYQAT+QGFEAKGFSR E E+LLRKSV+IA+EAR+IYLE+ TKD
Sbjct: 61  VHLDYLDAGANIISTASYQATLQGFEAKGFSRDESEDLLRKSVQIAIEARDIYLERCTKD 120

Query: 121 SWDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILAN 180
           SWDFT +G GSRRP L+AAS+GSYGAYLADGSEYSG+YG+SVTLE LK+FHRRRVQILAN
Sbjct: 121 SWDFTETGAGSRRPVLIAASIGSYGAYLADGSEYSGEYGESVTLETLKNFHRRRVQILAN 180

Query: 181 SGADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA 240
           +GADLIAFETIPNKLEAQAY+ELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA
Sbjct: 181 AGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA 240

Query: 241 SNQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDF 300
           S QVVAVGINCTPPRYIHGLI SIQ+VT+KPIV+YPNSGETYDGVTKQWLKSDG I EDF
Sbjct: 241 SKQVVAVGINCTPPRYIHGLILSIQEVTDKPIVVYPNSGETYDGVTKQWLKSDGMIGEDF 300

Query: 301 VSYVRKWKEAGASLFGGCCRTTPKTIRGIAKALSYKTF 338
           VSYVRKWKEAGASLFGGCCRTTP TIRGIAKALS KTF
Sbjct: 301 VSYVRKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

BLAST of MS007645 vs. ExPASy Swiss-Prot
Match: Q8LAX0 (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 SV=2)

HSP 1 Score: 507.7 bits (1306), Expect = 1.0e-142
Identity = 242/327 (74.01%), Postives = 280/327 (85.63%), Query Frame = 0

Query: 7   EMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLD 66
           E ++ MTDFL +CGG AVVDGGFATEL+RHGAD+NDPLWSAKCLI+SPHLV +VHLDYL+
Sbjct: 8   ETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYLE 67

Query: 67  AGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTGS 126
           +GANII+TASYQATIQGF AKG S  E E LLR+SVEI  EAREI+  + TK SWDF  +
Sbjct: 68  SGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAYA 127

Query: 127 GGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIA 186
           G  SRRP LVAASVGSYGAYLADGSEYSG YGDSV+ E LKDFHRRRVQILA SGADLIA
Sbjct: 128 GKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADLIA 187

Query: 187 FETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAV 246
           FETIPNKLEA+AY++LL+EE I+IPAWFSF SKDG++V  GDS+ +CA +AD+   VVA+
Sbjct: 188 FETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVVAI 247

Query: 247 GINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKW 306
           GINCT PRYIH LI S++++T KPIV+YPNSGE YDG+ K+W+KS+G  EEDFVSYV KW
Sbjct: 248 GINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVSKW 307

Query: 307 KEAGASLFGGCCRTTPKTIRGIAKALS 334
           ++AGASLFGGCCRTTP TIR IAK LS
Sbjct: 308 RDAGASLFGGCCRTTPNTIRAIAKVLS 334

BLAST of MS007645 vs. ExPASy Swiss-Prot
Match: Q9M1W4 (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.8e-129
Identity = 226/322 (70.19%), Postives = 265/322 (82.30%), Query Frame = 0

Query: 12  MTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLDAGANI 71
           M DFL+Q GG AV+DGG ATE ERHGADLNDPLWSAKCL++SPHL+  VHLDYL+AGA+I
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 72  ILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQY-TKDSWDFTGSGGGS 131
           I +ASYQATIQGFEAKGFSR E E LL+KSVEIA EAR  Y ++  T  S D        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMD---DKILK 128

Query: 132 RRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIAFETI 191
           +RP LVAASVGSYGAYLADGSEYSG YGDS+TLE LKDFHRRR+Q+LA SGADLIAFETI
Sbjct: 129 KRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETI 188

Query: 192 PNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAVGINC 251
           PNK+EAQA+++LL+E  ++IP WFSFNSKDG+NVVSGDSI +C SIA+   +VVAVGINC
Sbjct: 189 PNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINC 248

Query: 252 TPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKWKEAG 311
           TPPR+I GL+  I+KVT+KPI++YPNSGE+YD   K+W+++ G  +EDFVSYV KW +AG
Sbjct: 249 TPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAG 308

Query: 312 ASLFGGCCRTTPKTIRGIAKAL 333
            SL GGCCRTTP TIR I K L
Sbjct: 309 VSLLGGCCRTTPTTIRAIHKRL 327

BLAST of MS007645 vs. ExPASy Swiss-Prot
Match: P56707 (Selenocysteine methyltransferase OS=Astragalus bisulcatus OX=20406 GN=SMTA PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 9.3e-128
Identity = 224/326 (68.71%), Postives = 267/326 (81.90%), Query Frame = 0

Query: 12  MTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLDAGANI 71
           +TDFL Q G  AV+ GG  TEL+RHGADLNDPLWSAKCL+S PHL+R+VHLDYL+ GA+I
Sbjct: 6   ITDFLHQAGRAAVIAGGLGTELQRHGADLNDPLWSAKCLLSCPHLIRQVHLDYLENGADI 65

Query: 72  ILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTGSGGGSR 131
           I+TASYQATIQGF+AKGFS  EGE LLR+SVEIA EAR++Y ++  + S D   +G  SR
Sbjct: 66  IITASYQATIQGFKAKGFSDEEGEALLRRSVEIAREARDLYYQRCAESSSD---NGDDSR 125

Query: 132 ----RPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIAF 191
               RP L+A SVGSYGAYLADGSE+SG YGD++  E LKDFHRR+VQILA+SG DL+AF
Sbjct: 126 ILKQRPILIAGSVGSYGAYLADGSEFSGNYGDAIKSETLKDFHRRKVQILADSGVDLLAF 185

Query: 192 ETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAVG 251
           E +PNKLEAQAY++LL+EE I  PAWF+F SKDG NVVSGDSI +C SIA++ ++VVAVG
Sbjct: 186 EAVPNKLEAQAYADLLEEENIITPAWFAFTSKDGNNVVSGDSIEECGSIAESCDKVVAVG 245

Query: 252 INCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKWK 311
           INCTPPR+IH LI  ++KVT KPIVIYPNSGETYD + K+W ++ G  +EDFVSYV KW 
Sbjct: 246 INCTPPRFIHDLILLLKKVTAKPIVIYPNSGETYDAIRKEWGQNSGVTDEDFVSYVDKWC 305

Query: 312 EAGASLFGGCCRTTPKTIRGIAKALS 334
           E+GASL GGCCRTTP TIRGI K LS
Sbjct: 306 ESGASLVGGCCRTTPDTIRGIYKILS 328

BLAST of MS007645 vs. ExPASy Swiss-Prot
Match: Q4VNK0 (Selenocysteine Se-methyltransferase OS=Brassica oleracea var. italica OX=36774 GN=SMT PE=1 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 3.9e-126
Identity = 223/331 (67.37%), Postives = 261/331 (78.85%), Query Frame = 0

Query: 2   GKETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVH 61
           G   AE    M + L++ GG A++DGG ATELERHGADLNDPLWSAKCL++SPHL+  VH
Sbjct: 4   GNTKAETFYSMKELLKETGGYAIIDGGLATELERHGADLNDPLWSAKCLLTSPHLIHTVH 63

Query: 62  LDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSW 121
           LDYL+AGA+II +ASYQATIQGFEAKG+S  + E LLRKSVEIA EAR  Y ++   D  
Sbjct: 64  LDYLEAGADIISSASYQATIQGFEAKGYSIEKSESLLRKSVEIACEARSTYYDKCKDDD- 123

Query: 122 DFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSG 181
                    +RP LVAASVGSYGA+LADGSEYSG YGD +TLE LKDFHRRRVQ+LA SG
Sbjct: 124 ---DKKILKKRPILVAASVGSYGAFLADGSEYSGIYGDLITLETLKDFHRRRVQVLAESG 183

Query: 182 ADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASN 241
           AD+IAFETIPNKLEAQA++ELLDE   +IP WFSFNSKDG+NVVSGDSI +C +IA+A  
Sbjct: 184 ADIIAFETIPNKLEAQAFAELLDEGVAKIPGWFSFNSKDGVNVVSGDSIKECIAIAEACE 243

Query: 242 QVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVS 301
           +VVAVGINCTPPR+I GL+  I KVT+KPI++YPNSGE YD   K+W+++ G   EDFVS
Sbjct: 244 KVVAVGINCTPPRFIEGLVLEIAKVTSKPILVYPNSGERYDPERKEWVENTGVGNEDFVS 303

Query: 302 YVRKWKEAGASLFGGCCRTTPKTIRGIAKAL 333
           YV KW +AG SL GGCCRTTP TIR I K L
Sbjct: 304 YVEKWMDAGVSLLGGCCRTTPTTIRAIHKRL 330

BLAST of MS007645 vs. ExPASy Swiss-Prot
Match: Q9FUM9 (Homocysteine S-methyltransferase 2 OS=Zea mays OX=4577 GN=HMT-2 PE=2 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 3.1e-123
Identity = 214/314 (68.15%), Postives = 259/314 (82.48%), Query Frame = 0

Query: 20  GGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLDAGANIILTASYQA 79
           GG  V+DGG ATELE +GADLNDPLWSAKCL+SSPHL+R+VH+DYL+AGANII+TASYQA
Sbjct: 21  GGRLVLDGGLATELEANGADLNDPLWSAKCLLSSPHLIRKVHMDYLEAGANIIITASYQA 80

Query: 80  TIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTGSGGGSRRPTLVAAS 139
           TIQGFE+KGFS+ + E LL KSVEIALEARE++L+++ + S          + P LVAAS
Sbjct: 81  TIQGFESKGFSKEQSENLLTKSVEIALEAREMFLKEHLEKSTPI-------QHPVLVAAS 140

Query: 140 VGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIAFETIPNKLEAQAY 199
           +GSYGAYLADGSEYSG YG++ T E LKDFHRRR+Q+LA +G DLIAFETIPNKLEA+AY
Sbjct: 141 LGSYGAYLADGSEYSGDYGEAGTKEFLKDFHRRRLQVLAEAGPDLIAFETIPNKLEAEAY 200

Query: 200 SELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAVGINCTPPRYIHGL 259
            ELL+E  I IPAWFSFNSKDG+++VSGDS+ +C +IAD   +V AVGINCTPPR+IHGL
Sbjct: 201 VELLEECNINIPAWFSFNSKDGVHIVSGDSLIECTTIADKCAKVGAVGINCTPPRFIHGL 260

Query: 260 ISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKWKEAGASLFGGCCR 319
           I SI+KVT+KPI+IYPNSGE YDG  K+W++S G  + DFVSYV +W + GA L GGCCR
Sbjct: 261 ILSIRKVTDKPILIYPNSGERYDGEKKEWVESTGVSDGDFVSYVNEWCKDGAVLIGGCCR 320

Query: 320 TTPKTIRGIAKALS 334
           TTP TIR I + L+
Sbjct: 321 TTPNTIRAIHRTLN 327

BLAST of MS007645 vs. ExPASy TrEMBL
Match: A0A6J1CAF8 (homocysteine S-methyltransferase 3-like OS=Momordica charantia OX=3673 GN=LOC111009694 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.3e-190
Identity = 334/337 (99.11%), Postives = 334/337 (99.11%), Query Frame = 0

Query: 1   MGKETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRV 60
           MGKETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRV
Sbjct: 1   MGKETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRV 60

Query: 61  HLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDS 120
           HLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIAL AREIYLEQ TKDS
Sbjct: 61  HLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALGAREIYLEQCTKDS 120

Query: 121 WDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANS 180
           WDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANS
Sbjct: 121 WDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANS 180

Query: 181 GADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADAS 240
           GADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADAS
Sbjct: 181 GADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADAS 240

Query: 241 NQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFV 300
           NQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFV
Sbjct: 241 NQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFV 300

Query: 301 SYVRKWKEAGASLFGGCCRTTPKTIRGIAKALSYKTF 338
           SYVRKWKEAGASLFGGCCRTTPKTIRGIAKAL YKTF
Sbjct: 301 SYVRKWKEAGASLFGGCCRTTPKTIRGIAKALFYKTF 337

BLAST of MS007645 vs. ExPASy TrEMBL
Match: A0A1S3BMQ2 (homocysteine S-methyltransferase 3 OS=Cucumis melo OX=3656 GN=LOC103491241 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.1e-166
Identity = 291/338 (86.09%), Postives = 316/338 (93.49%), Query Frame = 0

Query: 1   MGKETAEMA-TFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRR 60
           MGK+  E A T++T FL++CGG  V+DGGFATELER GADLNDPLWSAKCL++SPHLVRR
Sbjct: 1   MGKQMPETATTYLTHFLQKCGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRR 60

Query: 61  VHLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKD 120
           VHLDYLDAGANII TASYQAT+QGFEAKGFSR E E+LLRKSV+IA+EAR+IYLE+ TKD
Sbjct: 61  VHLDYLDAGANIISTASYQATLQGFEAKGFSRDESEDLLRKSVQIAIEARDIYLERCTKD 120

Query: 121 SWDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILAN 180
           SWDFT +G GSRRP L+AAS+GSYGAYLADGSEYSG+YG+SVTLE LK+FHRRRVQILAN
Sbjct: 121 SWDFTETGAGSRRPVLIAASIGSYGAYLADGSEYSGEYGESVTLETLKNFHRRRVQILAN 180

Query: 181 SGADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA 240
           +GADLIAFETIPNKLEAQAY+ELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA
Sbjct: 181 AGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADA 240

Query: 241 SNQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDF 300
           S QVVAVGINCTPPRYIHGLI SIQ+VT+KPIV+YPNSGETYDGVTKQWLKSDG I EDF
Sbjct: 241 SKQVVAVGINCTPPRYIHGLILSIQEVTDKPIVVYPNSGETYDGVTKQWLKSDGMIGEDF 300

Query: 301 VSYVRKWKEAGASLFGGCCRTTPKTIRGIAKALSYKTF 338
           VSYVRKWKEAGASLFGGCCRTTP TIRGIAKALS KTF
Sbjct: 301 VSYVRKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

BLAST of MS007645 vs. ExPASy TrEMBL
Match: A0A6J1H601 (homocysteine S-methyltransferase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460426 PE=4 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.0e-166
Identity = 292/332 (87.95%), Postives = 308/332 (92.77%), Query Frame = 0

Query: 6   AEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYL 65
           AE  TFMTDFLRQCGG  V+DGG ATELERHGADLNDPLWSAKCL+SSPH+VRRVHLDYL
Sbjct: 21  AETTTFMTDFLRQCGGYGVIDGGLATELERHGADLNDPLWSAKCLVSSPHIVRRVHLDYL 80

Query: 66  DAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTG 125
           DAGANII TASYQATIQGFEAKGFSR E EELLRKSVEIALEAREIYLE+ TKDSWD+T 
Sbjct: 81  DAGANIITTASYQATIQGFEAKGFSRDESEELLRKSVEIALEAREIYLERCTKDSWDWTA 140

Query: 126 SGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLI 185
           +  GS RP LVAASVGSYGAYLADGSEYSGKYGDSV+LE LKDFHRRRVQILAN+GADLI
Sbjct: 141 TERGSTRPILVAASVGSYGAYLADGSEYSGKYGDSVSLETLKDFHRRRVQILANAGADLI 200

Query: 186 AFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVA 245
           AFETIPNKLEAQAY+ELLDEEGIEIPAWFSFNSKDGIN+VSGDSI+DCASIADAS +VVA
Sbjct: 201 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINMVSGDSITDCASIADASKRVVA 260

Query: 246 VGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRK 305
           VGINCTPPRYIHGLISSI++VTNKPI+IYPNSGETYDG TKQWLKSDG   EDFVSYV K
Sbjct: 261 VGINCTPPRYIHGLISSIREVTNKPILIYPNSGETYDGETKQWLKSDGMTGEDFVSYVSK 320

Query: 306 WKEAGASLFGGCCRTTPKTIRGIAKALSYKTF 338
           WKEAGASLFGGCCRTTP TI+GIAKALS K F
Sbjct: 321 WKEAGASLFGGCCRTTPNTIQGIAKALSSKAF 352

BLAST of MS007645 vs. ExPASy TrEMBL
Match: A0A6J1KIS7 (homocysteine S-methyltransferase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111494830 PE=4 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.4e-165
Identity = 283/329 (86.02%), Postives = 306/329 (93.01%), Query Frame = 0

Query: 8   MATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLDA 67
           M +FM DFL+QCGGC+V+DGGFATELERHGADLNDPLWSA CLISSPHL+RRVHLDYLDA
Sbjct: 1   MGSFMADFLQQCGGCSVIDGGFATELERHGADLNDPLWSATCLISSPHLIRRVHLDYLDA 60

Query: 68  GANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTGSG 127
           GANII TASYQATIQGFEAKGFS  E E+L+R+SVEIALEAR+IYL++ TKDSWDF GSG
Sbjct: 61  GANIICTASYQATIQGFEAKGFSTAESEDLVRRSVEIALEARDIYLQRCTKDSWDFLGSG 120

Query: 128 GGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIAF 187
           G   RP L+AAS+GSYGAYLADGSEY+G YGDSVTLE LKDFHRRRVQ+LAN+GADL+AF
Sbjct: 121 GSWGRPILIAASIGSYGAYLADGSEYNGNYGDSVTLETLKDFHRRRVQVLANAGADLLAF 180

Query: 188 ETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAVG 247
           ETIPNKLEAQAY+ELLDEEGI+IPAWF+FNSKDGINVVSGDS+SDCASIADAS QVVAVG
Sbjct: 181 ETIPNKLEAQAYTELLDEEGIQIPAWFAFNSKDGINVVSGDSLSDCASIADASKQVVAVG 240

Query: 248 INCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKWK 307
           INCTPPRYIHGLI SIQKVT KPI++YPNSGETYDG TKQWLKSDG IEEDFVSYVRKWK
Sbjct: 241 INCTPPRYIHGLILSIQKVTCKPILVYPNSGETYDGETKQWLKSDGMIEEDFVSYVRKWK 300

Query: 308 EAGASLFGGCCRTTPKTIRGIAKALSYKT 337
           EAGASLFGGCCRTTP TIRGIAKALS  T
Sbjct: 301 EAGASLFGGCCRTTPNTIRGIAKALSLNT 329

BLAST of MS007645 vs. ExPASy TrEMBL
Match: A0A5A7UQN6 (Homocysteine S-methyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold280G003160 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.6e-165
Identity = 289/334 (86.53%), Postives = 313/334 (93.71%), Query Frame = 0

Query: 4   ETAEMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLD 63
           ETA   T++T FL++CGG  V+DGGFATELER GADLNDPLWSAKCL++SPHLVRRVHLD
Sbjct: 3   ETA--TTYLTHFLQKCGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD 62

Query: 64  YLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDF 123
           YLDAGANII TASYQAT+QGFEAKGFSR E E+LLRKSV+IA+EAR+IYLE+ TKDSWDF
Sbjct: 63  YLDAGANIISTASYQATLQGFEAKGFSRDESEDLLRKSVQIAIEARDIYLERCTKDSWDF 122

Query: 124 TGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGAD 183
           T +G GSRRP L+AAS+GSYGAYLADGSEYSG+YG+SVTLE LK+FHRRRVQILAN+GAD
Sbjct: 123 TETGAGSRRPVLIAASIGSYGAYLADGSEYSGEYGESVTLETLKNFHRRRVQILANAGAD 182

Query: 184 LIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQV 243
           LIAFETIPNKLEAQAY+ELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADAS QV
Sbjct: 183 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV 242

Query: 244 VAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYV 303
           VAVGINCTPPRYIHGLI SIQ+VT+KPIV+YPNSGETYDGVTKQWLKSDG I EDFVSYV
Sbjct: 243 VAVGINCTPPRYIHGLILSIQEVTDKPIVVYPNSGETYDGVTKQWLKSDGMIGEDFVSYV 302

Query: 304 RKWKEAGASLFGGCCRTTPKTIRGIAKALSYKTF 338
           RKWKEAGASLFGGCCRTTP TIRGIAKALS KTF
Sbjct: 303 RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 334

BLAST of MS007645 vs. TAIR 10
Match: AT3G22740.1 (homocysteine S-methyltransferase 3 )

HSP 1 Score: 507.7 bits (1306), Expect = 7.3e-144
Identity = 242/327 (74.01%), Postives = 280/327 (85.63%), Query Frame = 0

Query: 7   EMATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLD 66
           E ++ MTDFL +CGG AVVDGGFATEL+RHGAD+NDPLWSAKCLI+SPHLV +VHLDYL+
Sbjct: 8   ETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYLE 67

Query: 67  AGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTGS 126
           +GANII+TASYQATIQGF AKG S  E E LLR+SVEI  EAREI+  + TK SWDF  +
Sbjct: 68  SGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAYA 127

Query: 127 GGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIA 186
           G  SRRP LVAASVGSYGAYLADGSEYSG YGDSV+ E LKDFHRRRVQILA SGADLIA
Sbjct: 128 GKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADLIA 187

Query: 187 FETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAV 246
           FETIPNKLEA+AY++LL+EE I+IPAWFSF SKDG++V  GDS+ +CA +AD+   VVA+
Sbjct: 188 FETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVVAI 247

Query: 247 GINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKW 306
           GINCT PRYIH LI S++++T KPIV+YPNSGE YDG+ K+W+KS+G  EEDFVSYV KW
Sbjct: 248 GINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVSKW 307

Query: 307 KEAGASLFGGCCRTTPKTIRGIAKALS 334
           ++AGASLFGGCCRTTP TIR IAK LS
Sbjct: 308 RDAGASLFGGCCRTTPNTIRAIAKVLS 334

BLAST of MS007645 vs. TAIR 10
Match: AT3G63250.1 (homocysteine methyltransferase 2 )

HSP 1 Score: 462.6 bits (1189), Expect = 2.7e-130
Identity = 226/322 (70.19%), Postives = 265/322 (82.30%), Query Frame = 0

Query: 12  MTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLDAGANI 71
           M DFL+Q GG AV+DGG ATE ERHGADLNDPLWSAKCL++SPHL+  VHLDYL+AGA+I
Sbjct: 9   MKDFLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADI 68

Query: 72  ILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQY-TKDSWDFTGSGGGS 131
           I +ASYQATIQGFEAKGFSR E E LL+KSVEIA EAR  Y ++  T  S D        
Sbjct: 69  ISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMD---DKILK 128

Query: 132 RRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIAFETI 191
           +RP LVAASVGSYGAYLADGSEYSG YGDS+TLE LKDFHRRR+Q+LA SGADLIAFETI
Sbjct: 129 KRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETI 188

Query: 192 PNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAVGINC 251
           PNK+EAQA+++LL+E  ++IP WFSFNSKDG+NVVSGDSI +C SIA+   +VVAVGINC
Sbjct: 189 PNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINC 248

Query: 252 TPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKWKEAG 311
           TPPR+I GL+  I+KVT+KPI++YPNSGE+YD   K+W+++ G  +EDFVSYV KW +AG
Sbjct: 249 TPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAG 308

Query: 312 ASLFGGCCRTTPKTIRGIAKAL 333
            SL GGCCRTTP TIR I K L
Sbjct: 309 VSLLGGCCRTTPTTIRAIHKRL 327

BLAST of MS007645 vs. TAIR 10
Match: AT3G63250.2 (homocysteine methyltransferase 2 )

HSP 1 Score: 383.3 bits (983), Expect = 2.1e-106
Identity = 191/278 (68.71%), Postives = 227/278 (81.65%), Query Frame = 0

Query: 56  LVRRVHLDYLDAGANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQ 115
           L+ +VHLDYL+AGA+II +ASYQATIQGFEAKGFSR E E LL+KSVEIA EAR  Y ++
Sbjct: 13  LMEQVHLDYLEAGADIISSASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDK 72

Query: 116 Y-TKDSWDFTGSGGGSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRV 175
             T  S D        +RP LVAASVGSYGAYLADGSEYSG YGDS+TLE LKDFHRRR+
Sbjct: 73  CGTSSSMD---DKILKKRPILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRL 132

Query: 176 QILANSGADLIAFETIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCA 235
           Q+LA SGADLIAFETIPNK+EAQA+++LL+E  ++IP WFSFNSKDG+NVVSGDSI +C 
Sbjct: 133 QVLAESGADLIAFETIPNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECI 192

Query: 236 SIADASNQVVAVGINCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGT 295
           SIA+   +VVAVGINCTPPR+I GL+  I+KVT+KPI++YPNSGE+YD   K+W+++ G 
Sbjct: 193 SIAENCEKVVAVGINCTPPRFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGV 252

Query: 296 IEEDFVSYVRKWKEAGASLFGGCCRTTPKTIRGIAKAL 333
            +EDFVSYV KW +AG SL GGCCRTTP TIR I K L
Sbjct: 253 GDEDFVSYVEKWMDAGVSLLGGCCRTTPTTIRAIHKRL 287

BLAST of MS007645 vs. TAIR 10
Match: AT3G25900.1 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 383.3 bits (983), Expect = 2.1e-106
Identity = 183/324 (56.48%), Postives = 238/324 (73.46%), Query Frame = 0

Query: 9   ATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLDAG 68
           +  + D +++CGGCAVVDGGFAT+LE HGA +NDPLWSA  LI +P L++RVH++YL+AG
Sbjct: 7   SALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHMEYLEAG 66

Query: 69  ANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTGSGG 128
           A+I++T+SYQATI GF ++G S  E E LL+KSVE+A+EAR+ + E+ +K S       G
Sbjct: 67  ADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVS-------G 126

Query: 129 GSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIAFE 188
            S    LVAAS+GSYGAYLADGSEYSG YG++V+L+ LKDFHRRR+Q+L  +G DL+AFE
Sbjct: 127 HSYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFE 186

Query: 189 TIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAVGI 248
           TIPNKLEAQA  ELL+EE ++IPAW  F S DG    SG+S  +C    + SN + AVGI
Sbjct: 187 TIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYAVGI 246

Query: 249 NCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKWKE 308
           NC PP++I  LI    K+T K IV+YPNSGE +DG  KQWL S    +++F  +  KW++
Sbjct: 247 NCAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRD 306

Query: 309 AGASLFGGCCRTTPKTIRGIAKAL 333
            GA L GGCCRTTP TI  I++ L
Sbjct: 307 LGAKLIGGCCRTTPSTINAISRDL 323

BLAST of MS007645 vs. TAIR 10
Match: AT3G25900.3 (Homocysteine S-methyltransferase family protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.6e-95
Identity = 172/324 (53.09%), Postives = 225/324 (69.44%), Query Frame = 0

Query: 9   ATFMTDFLRQCGGCAVVDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYLDAG 68
           +  + D +++CGGCAVVDGGFAT+LE HGA +NDPLWSA  LI +P L++RVH++YL+AG
Sbjct: 7   SALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHMEYLEAG 66

Query: 69  ANIILTASYQATIQGFEAKGFSRVEGEELLRKSVEIALEAREIYLEQYTKDSWDFTGSGG 128
           A+I++T+SYQATI GF ++G S  E E LL+KSVE+A+EAR+ + E+ +K S       G
Sbjct: 67  ADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVS-------G 126

Query: 129 GSRRPTLVAASVGSYGAYLADGSEYSGKYGDSVTLEMLKDFHRRRVQILANSGADLIAFE 188
            S    LVAAS+GSYGAYLADGSEYSG YG++V+L+ LKDFHRRR+Q+L  +G DL+AFE
Sbjct: 127 HSYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLLAFE 186

Query: 189 TIPNKLEAQAYSELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASNQVVAVGI 248
           TIPNKLEAQA  ELL+EE ++IPAW  F S DG    SG+S  +C    + SN + A   
Sbjct: 187 TIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA--- 246

Query: 249 NCTPPRYIHGLISSIQKVTNKPIVIYPNSGETYDGVTKQWLKSDGTIEEDFVSYVRKWKE 308
                            +T K IV+YPNSGE +DG  KQWL S    +++F  +  KW++
Sbjct: 247 -----------------LTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATKWRD 303

Query: 309 AGASLFGGCCRTTPKTIRGIAKAL 333
            GA L GGCCRTTP TI  I++ L
Sbjct: 307 LGAKLIGGCCRTTPSTINAISRDL 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138564.14.8e-19099.11homocysteine S-methyltransferase 3-like [Momordica charantia][more]
XP_038906597.13.3e-17090.21homocysteine S-methyltransferase 3-like [Benincasa hispida][more]
XP_023549248.11.7e-16688.55homocysteine S-methyltransferase 3-like [Cucurbita pepo subsp. pepo][more]
KAG7013538.13.7e-16688.25Homocysteine S-methyltransferase 3, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_008449335.16.3e-16686.09PREDICTED: homocysteine S-methyltransferase 3 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q8LAX01.0e-14274.01Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=HMT3 PE=1 ... [more]
Q9M1W43.8e-12970.19Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=HMT-2 PE=1... [more]
P567079.3e-12868.71Selenocysteine methyltransferase OS=Astragalus bisulcatus OX=20406 GN=SMTA PE=1 ... [more]
Q4VNK03.9e-12667.37Selenocysteine Se-methyltransferase OS=Brassica oleracea var. italica OX=36774 G... [more]
Q9FUM93.1e-12368.15Homocysteine S-methyltransferase 2 OS=Zea mays OX=4577 GN=HMT-2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CAF82.3e-19099.11homocysteine S-methyltransferase 3-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3BMQ23.1e-16686.09homocysteine S-methyltransferase 3 OS=Cucumis melo OX=3656 GN=LOC103491241 PE=4 ... [more]
A0A6J1H6014.0e-16687.95homocysteine S-methyltransferase 3-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KIS73.4e-16586.02homocysteine S-methyltransferase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111494... [more]
A0A5A7UQN67.6e-16586.53Homocysteine S-methyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT3G22740.17.3e-14474.01homocysteine S-methyltransferase 3 [more]
AT3G63250.12.7e-13070.19homocysteine methyltransferase 2 [more]
AT3G63250.22.1e-10668.71homocysteine methyltransferase 2 [more]
AT3G25900.12.1e-10656.48Homocysteine S-methyltransferase family protein [more]
AT3G25900.31.6e-9553.09Homocysteine S-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003726Homocysteine-binding domainPFAMPF02574S-methyl_transcoord: 24..332
e-value: 7.3E-78
score: 262.2
IPR003726Homocysteine-binding domainPROSITEPS50970HCYcoord: 11..332
score: 48.24057
IPR036589Homocysteine-binding domain superfamilyGENE3D3.20.20.330coord: 14..331
e-value: 3.7E-104
score: 350.4
IPR036589Homocysteine-binding domain superfamilySUPERFAMILY82282Homocysteine S-methyltransferasecoord: 20..333
IPR017226Betaine-homocysteine S-methyltransferase, BHMTPIRSFPIRSF037505BHMTcoord: 288..336
e-value: 0.31
score: 8.2
coord: 14..110
e-value: 2.4E-7
score: 28.2
coord: 186..269
e-value: 410.0
score: -2.1
NoneNo IPR availablePANTHERPTHR46015:SF8HOMOCYSTEINE S-METHYLTRANSFERASE 2-LIKE ISOFORM X1coord: 7..335
NoneNo IPR availablePANTHERPTHR46015ZGC:172121coord: 7..335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS007645.1MS007645.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
molecular_function GO:0047150 betaine-homocysteine S-methyltransferase activity
molecular_function GO:0008270 zinc ion binding