MS007342 (gene) Bitter gourd (TR) v1

Overview


Name	MS007342
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	alpha-amylase/subtilisin inhibitor-like
Location	scaffold25: 2528573 .. 2529125 (+)
RNA-Seq Expression	MS007342
Synteny	MS007342

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGATGGTGTTGCAAAAGTTGGTTGCCTTGTGCCTGTTCGCAGCCATATTTTCGGCCGCAGCCGACGTTCCTGTGCTCGACACCAACGGCGATCGCATTGTATCAGGCGCACAATACTACATAAAGCCTGCAATTGTTGGTAGAGGCGGAGCTCTCACTTTGGTCGCCAGAAAAAATGGCAAGCCATGTCCGAAATATGTCGGTCAGGTAGAGCATCCTGATCAGAAAGGAACCCCAGTTGTTGGCTCCGGTGACCAACGAAGGTTGGCTTGCACATATTACGTTTCAAACGTCTGATTCTTGCTCAAAACTGACCATGTGGGGGCTAGGCGATCAGGAGAATGTGTTGGGAAGGAGGCTGATCACGACGGGGTCGGAGCTTTTGGTTGAAGTCTTGTTCAAGATTGTGAAAAGCAATGGACTTTACCATATTCTTTGGTGTACTTCAGAAGATCACAATCCTCAATGTGGAAGTGCTGGTATCTTGAGTGAGGATGGGGTGAGTTTGTTCGCTTTGGATGGCCCTGAGCTTCCTTTTGTCTTTGAGAAAGTT

mRNA sequence

AAGATGGTGTTGCAAAAGTTGGTTGCCTTGTGCCTGTTCGCAGCCATATTTTCGGCCGCAGCCGACGTTCCTGTGCTCGACACCAACGGCGATCGCATTGTATCAGGCGCACAATACTACATAAAGCCTGCAATTGTTGGTAGAGGCGGAGCTCTCACTTTGGTCGCCAGAAAAAATGGCAAGCCATGTCCGAAATATGTCGGTCAGGTAGAGCATCCTGATCAGAAAGGAACCCCAGTTTTGGCTCCGGTGACCAACGAAGGTTGGCTTGCACATATTACGTTTCAAACGTCTGATTCTTGCTCAAAACTGACCATGTGGGGGCTAGGCGATCAGGAGAATGTGTTGGGAAGGAGGCTGATCACGACGGGGTCGGAGCTTTTGGTTGAAGTCTTGTTCAAGATTGTGAAAAGCAATGGACTTTACCATATTCTTTGGTGTACTTCAGAAGATCACAATCCTCAATGTGGAAGTGCTGGTATCTTGAGTGAGGATGGGGTGAGTTTGTTCGCTTTGGATGGCCCTGAGCTTCCTTTTGTCTTTGAGAAAGTT

Coding sequence (CDS)

AAGATGGTGTTGCAAAAGTTGGTTGCCTTGTGCCTGTTCGCAGCCATATTTTCGGCCGCAGCCGACGTTCCTGTGCTCGACACCAACGGCGATCGCATTGTATCAGGCGCACAATACTACATAAAGCCTGCAATTGTTGGTAGAGGCGGAGCTCTCACTTTGGTCGCCAGAAAAAATGGCAAGCCATGTCCGAAATATGTCGGTCAGGTAGAGCATCCTGATCAGAAAGGAACCCCAGTTTTGGCTCCGGTGACCAACGAAGGTTGGCTTGCACATATTACGTTTCAAACGTCTGATTCTTGCTCAAAACTGACCATGTGGGGGCTAGGCGATCAGGAGAATGTGTTGGGAAGGAGGCTGATCACGACGGGGTCGGAGCTTTTGGTTGAAGTCTTGTTCAAGATTGTGAAAAGCAATGGACTTTACCATATTCTTTGGTGTACTTCAGAAGATCACAATCCTCAATGTGGAAGTGCTGGTATCTTGAGTGAGGATGGGGTGAGTTTGTTCGCTTTGGATGGCCCTGAGCTTCCTTTTGTCTTTGAGAAAGTT

Protein sequence

KMVLQKLVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQVEHPDQKGTPVLAPVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVLGRRLITTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGPELPFVFEKV

Homology

[Close]

BLAST of MS007342 vs. NCBI nr
Match: XP_022150757.1 (alpha-amylase/subtilisin inhibitor-like [Momordica charantia])

HSP 1 Score: 363.2 bits (931), Expect = 1.3e-96
Identity = 179/186 (96.24%), Postives = 181/186 (97.31%), Query Frame = 0


Query: 2   MVLQKLVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGK 61
           MVLQKLVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGK
Sbjct: 1   MVLQKLVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGK 60

Query: 62  PCPKYVGQVEHPDQKGTPV---LAPVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVLGR 121
           PCPKYVGQVEHPDQKGTPV   LAPVTNEGWLAHITFQTSDSCSKLTMWGLGD+ENV GR
Sbjct: 61  PCPKYVGQVEHPDQKGTPVKFMLAPVTNEGWLAHITFQTSDSCSKLTMWGLGDRENVSGR 120

Query: 122 RLITTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGPELP 181
           RLIT+GSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSL ALDGPELP
Sbjct: 121 RLITSGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLLALDGPELP 180

Query: 182 FVFEKV 185
           FVFEKV
Sbjct: 181 FVFEKV 186

[Close]

BLAST of MS007342 vs. NCBI nr
Match: XP_023545477.1 (miraculin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 139.4 bits (350), Expect = 3.1e-29
Identity = 77/182 (42.31%), Postives = 102/182 (56.04%), Query Frame = 0


Query: 10  LCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQ 69
           L +F AI S A   PVLDT+G  +  G +YYIKPAI    G LTL +R N  PCP YVGQ
Sbjct: 12  LLVFMAITSTAQLPPVLDTDGQPLQRGVEYYIKPAITDVAGNLTLKSRSNA-PCPLYVGQ 71

Query: 70  VEHPDQK------GTPVLA--PVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVLGRRLI 129
                         TP++A   +  E    ++ FQ S +C   T W + + E+  GRR +
Sbjct: 72  EPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFV 131

Query: 130 TTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGPELPFVF 184
             G++     +F+I ++NG+Y+I+WC      P+CGSAGIL EDGV L ALDG   PF F
Sbjct: 132 GVGNDNSPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEF 191

[Close]

BLAST of MS007342 vs. NCBI nr
Match: XP_022946294.1 (miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita moschata])

HSP 1 Score: 138.3 bits (347), Expect = 6.8e-29
Identity = 77/182 (42.31%), Postives = 102/182 (56.04%), Query Frame = 0


Query: 10  LCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQ 69
           L +F AI S A   PVLDT+G  +  G +YYIKPAI    G LTL +R N  PCP YVGQ
Sbjct: 12  LLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNA-PCPLYVGQ 71

Query: 70  VEHPDQK------GTPVLA--PVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVLGRRLI 129
                         TP++A   +  E    ++ FQ S +C   T W + + E+  GRR +
Sbjct: 72  EPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFV 131

Query: 130 TTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGPELPFVF 184
             G++     +F+I ++NG+Y+I+WC      P+CGSAGIL EDGV L ALDG   PF F
Sbjct: 132 GVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEF 191

[Close]

BLAST of MS007342 vs. NCBI nr
Match: XP_022999136.1 (miraculin-like [Cucurbita maxima])

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-28
Identity = 77/182 (42.31%), Postives = 101/182 (55.49%), Query Frame = 0


Query: 10  LCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQ 69
           L +F AI S A   PVLDT+G  +  G +YYIKPAI    G LTL  R N  PCP YVGQ
Sbjct: 12  LLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSNA-PCPLYVGQ 71

Query: 70  VEHPDQK------GTPVLA--PVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVLGRRLI 129
                         TP++A   +  E    ++ FQ S +C   T W + + E+  GRR +
Sbjct: 72  EPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFV 131

Query: 130 TTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGPELPFVF 184
             G++     +F+I ++NG+Y+I+WC      P+CGSAGIL EDGV L ALDG   PF F
Sbjct: 132 GVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEF 191

[Close]

BLAST of MS007342 vs. NCBI nr
Match: XP_022150789.1 (miraculin-like [Momordica charantia])

HSP 1 Score: 136.7 bits (343), Expect = 2.0e-28
Identity = 85/188 (45.21%), Postives = 105/188 (55.85%), Query Frame = 0


Query: 7   LVALCL-FAAIFSAAAD-VPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCP 66
           L +LCL F AI S+ A   PVLDTNG  +  G +YYIKPAI    G LTL +R    PCP
Sbjct: 10  LSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRA-PCP 69

Query: 67  KYVGQVEHPDQK-GTPVL-------APVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVL 126
            YVGQ      + G PV+         +  EG    + FQ S +C  LT W + + E   
Sbjct: 70  LYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVEETEAAT 129

Query: 127 GRRLITTGSELLVEVLFKIVKSN-GLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGP 184
           GRRL+ TG +     +F+I +SN G+Y+I WC      P+CGSAGIL EDGV L ALDG 
Sbjct: 130 GRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLALDGS 189

[Close]

BLAST of MS007342 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.6e-23
Identity = 68/176 (38.64%), Postives = 96/176 (54.55%), Query Frame = 0


Query: 25  VLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQVEHPDQKGTPV---- 84
           V+DT+G+ +    +Y+I+PAI G GG  TLV   NG PCP +VG        G  V    
Sbjct: 27  VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT-GNG-PCPLHVGLDNTEGTLGVAVKFTP 86

Query: 85  LAPVTNEGWL-----AHITFQTSDSCSKLTMWGLGDQENVLGRRLITTGSELLVEV---L 144
            AP  ++  +       +TF TS SC + T W LG+++   GRRLI TG +         
Sbjct: 87  FAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDATSGRRLIVTGRDNGAGSQGNF 146

Query: 145 FKIV--KSNGLYHILWCTSE---DHNPQCGSAGILSEDGVSLFALDGPELPFVFEK 184
           F+IV  ++ G Y+I WC +E       QCG+ G++ E+G +L ALDG  LP VF+K
Sbjct: 147 FRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENGKNLLALDGDALPVVFQK 200

[Close]

BLAST of MS007342 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.4e-21
Identity = 65/176 (36.93%), Postives = 94/176 (53.41%), Query Frame = 0


Query: 25  VLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQVEHPDQKGTPVLAP- 84
           V+DT+G+ + +   Y+I+PAI G GG+LTLV R +   CP  VG      Q    +L+P 
Sbjct: 27  VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTRNS---CPFNVGLDPDAPQGFAVLLSPF 86

Query: 85  VTNE-------GWLAHITFQTSDSCSKLTMWGLGDQENVLGRRLITTGSELLV----EVL 144
           V+N        G    + FQ   SC + T W LG+++   GRR I TG +          
Sbjct: 87  VSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFIITGRDDSTVGSYGNF 146

Query: 145 FKIVK--SNGLYHILWCTSE---DHNPQCGSAGILSEDGVSLFALDGPELPFVFEK 184
           F+IV+  S G+++I WC +E       +CG+ GI+ E+G  L ALDG  LP  F+K
Sbjct: 147 FRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALDGSALPVAFQK 199

[Close]

BLAST of MS007342 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.0e-19
Identity = 70/198 (35.35%), Postives = 102/198 (51.52%), Query Frame = 0


Query: 7   LVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKY 66
           L  +CLF    + A    VLDT G+ +    +Y+I+P I  +GG  T+V+R   + CP +
Sbjct: 13  LAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR--NESCPLH 72

Query: 67  VGQVEHPDQKGTPV----LAP--------VTNEGWLAHITFQTSDSCSKLTMWGLGDQEN 126
           VG       +G  V     AP        V  +     ITFQ S SC + T W LG+++ 
Sbjct: 73  VGLELTGLGRGLVVKFTPFAPHHDFDDVRVNRD---LRITFQASSSCVQSTEWRLGEKDT 132

Query: 127 VLGRRLITTGSELLVE----VLFKIVKS--NGLYHILWCTSE---DHNPQCGSAGILSED 184
             GRRLI TG++          F+IV++   G+Y+I WC +E       +CG+  +L+E+
Sbjct: 133 KSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLNEN 192

[Close]

BLAST of MS007342 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.3e-11
Identity = 59/184 (32.07%), Postives = 82/184 (44.57%), Query Frame = 0


Query: 20  AADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQVEHPDQKGTP 79
           AA+ PVLDT+GD + +G QYY+  +I G GG    + R  G+ CP+ V Q       GTP
Sbjct: 26  AANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDLDNGTP 85

Query: 80  VL-------APVTNEGWLAHITFQ--TSDSCSKLTMWGLGDQENVLGRRLITT------- 139
           V+         V       +I F       CS  T+W L + +N  G+  +TT       
Sbjct: 86  VIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDGVKGEP 145

Query: 140 GSELLVEVLFKIVKSNGL-YHILWCTS--EDHNPQCGSAGILS-EDGVSLFALDGPELPF 184
           G   L    FKI K+  L Y   +C S  +     C   G  S +DG    AL   E  +
Sbjct: 146 GPNTLCS-WFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLALSDNEWAW 205

[Close]

BLAST of MS007342 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 1.9e-10
Identity = 59/194 (30.41%), Postives = 79/194 (40.72%), Query Frame = 0


Query: 7   LVALCLFAAIFS-AAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPK 66
           L+ L L A  FS +AA  PV DT G  + +   YY+ PA  G GG LT+  R    PCP 
Sbjct: 8   LILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV--LPCPL 67

Query: 67  YVGQVEHPDQKGTPV--------LAP---VTNEGWLAHITFQTSDSCSKLTMWGLGDQEN 126
            V Q     +KG PV         AP            I F  +  C + T W +GD E 
Sbjct: 68  LVAQETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGD-EP 127

Query: 127 VLGRRLITTG-----SELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSL 184
           + G R + TG     S    E  F++ K  G Y ++ C        C   G+  +   + 
Sbjct: 128 LTGARRVVTGPLIGPSPSGRENAFRVEKYGGGYKLVSC-----RDSCQDLGVSRDGARAW 187

[Close]

BLAST of MS007342 vs. ExPASy TrEMBL
Match: A0A6J1DBL5 (alpha-amylase/subtilisin inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111018817 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 6.3e-97
Identity = 179/186 (96.24%), Postives = 181/186 (97.31%), Query Frame = 0


Query: 2   MVLQKLVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGK 61
           MVLQKLVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGK
Sbjct: 1   MVLQKLVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGK 60

Query: 62  PCPKYVGQVEHPDQKGTPV---LAPVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVLGR 121
           PCPKYVGQVEHPDQKGTPV   LAPVTNEGWLAHITFQTSDSCSKLTMWGLGD+ENV GR
Sbjct: 61  PCPKYVGQVEHPDQKGTPVKFMLAPVTNEGWLAHITFQTSDSCSKLTMWGLGDRENVSGR 120

Query: 122 RLITTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGPELP 181
           RLIT+GSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSL ALDGPELP
Sbjct: 121 RLITSGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLLALDGPELP 180

Query: 182 FVFEKV 185
           FVFEKV
Sbjct: 181 FVFEKV 186

[Close]

BLAST of MS007342 vs. ExPASy TrEMBL
Match: A0A6J1G3G3 (miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 3.3e-29
Identity = 77/182 (42.31%), Postives = 102/182 (56.04%), Query Frame = 0


Query: 10  LCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQ 69
           L +F AI S A   PVLDT+G  +  G +YYIKPAI    G LTL +R N  PCP YVGQ
Sbjct: 12  LLVFMAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITDVAGNLTLKSRSNA-PCPLYVGQ 71

Query: 70  VEHPDQK------GTPVLA--PVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVLGRRLI 129
                         TP++A   +  E    ++ FQ S +C   T W + + E+  GRR +
Sbjct: 72  EPIASTNIGLPVTFTPIVAGEDIIEESMGFNVVFQASSTCITSTQWRVDEMESATGRRFV 131

Query: 130 TTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGPELPFVF 184
             G++     +F+I ++NG+Y+I+WC      P+CGSAGIL EDGV L ALDG   PF F
Sbjct: 132 GVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEF 191

[Close]

BLAST of MS007342 vs. ExPASy TrEMBL
Match: A0A6J1KC85 (miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 5.6e-29
Identity = 77/182 (42.31%), Postives = 101/182 (55.49%), Query Frame = 0


Query: 10  LCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCPKYVGQ 69
           L +F AI S A   PVLDT+G  +  G +YYIKPAI    G LTL  R N  PCP YVGQ
Sbjct: 12  LLVFLAITSTAQLPPVLDTDGQPLRRGVEYYIKPAITNVAGNLTLKNRSNA-PCPLYVGQ 71

Query: 70  VEHPDQK------GTPVLA--PVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVLGRRLI 129
                         TP++A   +  E    ++ FQ S +C   T W + + E+  GRR +
Sbjct: 72  EPVASTNIGLPVTFTPIVAGEDIIEESMGFNVAFQASSTCITSTQWRVDEMESATGRRFV 131

Query: 130 TTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGPELPFVF 184
             G++     +F+I ++NG+Y+I+WC      P+CGSAGIL EDGV L ALDG   PF F
Sbjct: 132 GVGNDNGPTGIFRIDRNNGVYNIVWCPEMVGRPRCGSAGILVEDGVRLLALDGNAFPFEF 191

[Close]

BLAST of MS007342 vs. ExPASy TrEMBL
Match: A0A6J1DB43 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 9.6e-29
Identity = 85/188 (45.21%), Postives = 105/188 (55.85%), Query Frame = 0


Query: 7   LVALCL-FAAIFSAAAD-VPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNGKPCP 66
           L +LCL F AI S+ A   PVLDTNG  +  G +YYIKPAI    G LTL +R    PCP
Sbjct: 10  LSSLCLIFMAISSSTAQPPPVLDTNGQALRRGIEYYIKPAITDVAGNLTLKSRSRA-PCP 69

Query: 67  KYVGQVEHPDQK-GTPVL-------APVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVL 126
            YVGQ      + G PV+         +  EG    + FQ S +C  LT W + + E   
Sbjct: 70  LYVGQEPVASMEIGLPVIFTPYAADEDIIREGMDFKVVFQASSTCIMLTQWRVEETEAAT 129

Query: 127 GRRLITTGSELLVEVLFKIVKSN-GLYHILWCTSEDHNPQCGSAGILSEDGVSLFALDGP 184
           GRRL+ TG +     +F+I +SN G+Y+I WC      P+CGSAGIL EDGV L ALDG 
Sbjct: 130 GRRLVGTGDDNGPTGMFRIDRSNLGVYNIGWCPEMFGRPRCGSAGILVEDGVRLLALDGS 189

[Close]

BLAST of MS007342 vs. ExPASy TrEMBL
Match: A0A5D3D468 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520 PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 2.1e-28
Identity = 81/194 (41.75%), Postives = 104/194 (53.61%), Query Frame = 0


Query: 2   MVLQKLVAL----CLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVAR 61
           M+L K +A+    CLF AI S A   PVLDTNG  +  G +YYI PAI   GG LTL +R
Sbjct: 2   MMLHKSLAIFGYACLFMAITSTAQSPPVLDTNGQPLRRGVEYYISPAITDVGGNLTLKSR 61

Query: 62  KNGKPCPKYVGQVEHPDQK-GTPVL-------APVTNEGWLAHITFQTSDSCSKLTMWGL 121
            N  PCP +VGQ        G PV          + +EG   +I F+   +C+  T W +
Sbjct: 62  SNA-PCPLFVGQEPVTSTNIGLPVTFRPTEAGKDIIDEGTSLNIVFEALSTCATSTQWRV 121

Query: 122 GDQENVLGRRLITTGSELLVEVLFKIVKSNGLYHILWCTSEDHNPQCGSAGILSEDGVSL 181
              E+  GRR +  G E     +F I + NG Y+I+WC +    P+CG AGIL E+GV L
Sbjct: 122 DATESDTGRRFVGIGDEDGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRL 181

Query: 182 FALDGPELPFVFEK 184
            ALDG   PF F K
Sbjct: 182 VALDGDAFPFEFIK 194

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BLAST of MS007342 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.0e-10
Identity = 52/195 (26.67%), Postives = 85/195 (43.59%), Query Frame = 0


Query: 4   LQKLVALCLFAAIF-------SAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVA 63
           +  L+ + L  A+F       + AA  PV D NG  +++G  YYI P I GRGG LT+  
Sbjct: 1   MSSLLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSN 60

Query: 64  RKNGKPCPKYVGQVEHPDQKGTPVLAPVTNEGWLAHITFQTSDSCSKLTMWGLGDQENVL 123
            K  + CP  V Q +    +G PV     ++     ++   +   S  ++W L + +   
Sbjct: 61  LKT-ETCPTSVIQDQFEVSQGLPVKFSPYDKSRTIPVSTDVNIKFSPTSIWELANFDETT 120

Query: 124 GRRLITT------GSELLVEVLFKIVKSNGLYHILWCTSEDH--NPQCGSAGILSEDGVS 183
            +  I+T        +  V+  FKI K    Y I +C +  +     C   G+  +DG  
Sbjct: 121 KQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQDGKR 180

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BLAST of MS007342 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 62.4 bits (150), Expect = 4.4e-10
Identity = 56/201 (27.86%), Postives = 88/201 (43.78%), Query Frame = 0


Query: 1   KMVLQKLVALCLFAAIFSAAADVPVLDTNGDRIVSGAQYYIKPAIVGRGGALTLVARKNG 60
           K +  K   +    A+ ++ A   V+D +G+ +     YY+ P I GRGG LTL  R  G
Sbjct: 6   KTMNPKFYLVLALTAVLASNAYGAVVDIDGNAMFH-ESYYVLPVIRGRGGGLTLAGR-GG 65

Query: 61  KPCPKYVGQVEHPDQKGTPV--------LAPVTNEGWLAHITFQTSDSCSKLTMWGLGDQ 120
           +PCP  + Q      +G PV        +A V     L   T   +  C + T W +G+ 
Sbjct: 66  QPCPYDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEF 125

Query: 121 ENVLGRRLITTG------SELLVEVLFKIVKS-NGLYHILWC--TSEDHNPQCGSAGI-L 180
           ++   +  +  G       +  ++  FKI KS    Y  ++C  T +  NP+C   GI +
Sbjct: 126 DHERKQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFI 185

Query: 181 SEDGVSLFALDGPELPFVFEK 184
            E GV   AL       +F+K
Sbjct: 186 DELGVRRLALSDKPFLVMFKK 204

The following BLAST results are available for this feature:

BLAST of MS007342 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150757.1	1.3e-96	96.24	alpha-amylase/subtilisin inhibitor-like [Momordica charantia]	[more]
XP_023545477.1	3.1e-29	42.31	miraculin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022946294.1	6.8e-29	42.31	miraculin-like [Cucurbita moschata] >XP_022946295.1 miraculin-like [Cucurbita mo...	[more]
XP_022999136.1	1.2e-28	42.31	miraculin-like [Cucurbita maxima]	[more]
XP_022150789.1	2.0e-28	45.21	miraculin-like [Momordica charantia]	[more]

BLAST of MS007342 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A072TH68	7.6e-23	38.64	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
G7LCV7	1.4e-21	36.93	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	1.0e-19	35.35	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
P32765	1.3e-11	32.07	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
P29421	1.9e-10	30.41	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of MS007342 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DBL5	6.3e-97	96.24	alpha-amylase/subtilisin inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1G3G3	3.3e-29	42.31	miraculin-like OS=Cucurbita moschata OX=3662 GN=LOC111450419 PE=3 SV=1	[more]
A0A6J1KC85	5.6e-29	42.31	miraculin-like OS=Cucurbita maxima OX=3661 GN=LOC111493610 PE=3 SV=1	[more]
A0A6J1DB43	9.6e-29	45.21	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018852 PE=3 SV=1	[more]
A0A5D3D468	2.1e-28	41.75	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00520...	[more]

BLAST of MS007342 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	2.0e-10	26.67	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	4.4e-10	27.86	kunitz trypsin inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 63..83 score: 22.04 coord: 24..53 score: 40.07
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 24..184 e-value: 6.8E-14 score: 62.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 25..183 e-value: 2.3E-25 score: 89.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 9..183
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 25..41
None	No IPR available	GENE3D	2.80.10.50		coord: 19..184 e-value: 1.0E-30 score: 108.6
None	No IPR available	PANTHER	PTHR33107:SF40	TRYPSIN INHIBITOR A	coord: 9..183
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 24..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS007342.1	MS007342.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004866	endopeptidase inhibitor activity