MS007334 (gene) Bitter gourd (TR) v1

Overview


Name	MS007334
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type domain-containing protein
Location	scaffold25: 2466101 .. 2469231 (-)
RNA-Seq Expression	MS007334
Synteny	MS007334

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTGCAGAGCACTGCGCAAATGGAGATGGAGACGGCTAGTTCTTCTTACGACGATACGAACCCCTGCCCGATCTGTCTCGGATCCATAATTCGACCTTCTTACCTCGACAAATGCTTCCGTACTTCTCTATCTCTCTCAATCTCATCATTCTCTTGATTTTCTTGAATTTTCCGGTAATCCAACAGATAACTATTTTGAAATATTGTTCATTTGTACGAATTAGGGTTTTTTAATCATGTAGTTACCTACTTGGAAATGACCCTTTATGTGTTACTGCCACTTTATTGTCCTTTTAGGGGATTTCAGCTCCTAGCTCTTTCTCATATTTGCTGGACGAAATTGTTCGATCACTATAGCATGTTGTAAGATAGAATTAAGGGGTTTCATTTGGGATAAATTAGCTAATTCTTTACTTTGAGTAGACATAAAATTCTTCTACAGATGCTCAATTTTATTCAGGCAAGCCGTTTCTTCTTCAAGAAAAATAGAAACAACAGTAAGTTTGCTATGGGTGGCTTTGGTACGTTGAGTGTACTCATTGATGGACTCTGTTACCTTCTTTGAGATGAATTTGATCATATATATATATATATATTTGTATCCATGAGTGTCTAGGCTAGCTTATGCGCATCTCAACTAAGCTTACGGGACAACCGCCTGACCCTAATATATTTGGTTGATTGTTGAGGAAATATGTAGGACATTAAATTCTAGGTTGGTGGCCACCATGGGGTTTGAGCCTTCCTTATAAGTGGACTTGAATATTGATTTTAGTTACGTTGAAAAATGCATGGAACAATGATATTGTGTTGATCACTAGTAATTCTAAAGTTTGAGCTTTAAAGTTTGAGTGAATTTGGTGATTGAATTTACTCTTAAAAAAGTTATCCATCTGTTCATTCATGTTAAGTACAATTTACTATTAGGGAATGTCAATATAGTTTTCCAGTTTGAATGCTTTTAATTCTATTTCTTGTCTTGCAGATAAGTTTTGTTACAATTGCATTGTGCAATGGACCAAAGTGGTTTCTGGCAAGCGCTCCTGCATTCTTTCGTCCATTAAGTGTCCTTTATGCAAGGTTATATCTTCCGTCCCCTGTTGTATAAGTATTGTTTTATATTAGCATTCCATACTTTTTGTATTGAATTTCTTATCATATTGAATCACCAGACAGAAAGTTCTTCTATAATACATGGATTAGATGGACATTGTTTCCAAAGGCATTACGTTAATCAGGATTTTCAGGATAGGTATCTCCCTGTTGACACTTTTCCTTAGATTTCCTATTCTTATTTTCTCTGCATTAATTGACTATGTTTTTATCACTGATGCTATTTGCAGCTTTATCTTATCAAAAGCCCACAAGTATAGATTACAGTGCTATTATACTGAACCAGGTTCATGTTCTGCTAGTCATTATACTCATTATGTGGATGTTTGATGCTCTGACATTTACTTCATGATTACTCAAAGTTATGTATTTCCCCTAATTGTCTACGTGTAGGTTTCTTGGAGGACATATTCAATGTACAAAGATACTGGAAATTACGAAAGTATCTCCAAGCAAACCAATGGCTTGAAGTTTGGTTGAAAAGGGAACTTCAAGCTTTGATTCAGGTTCCTGACTTAGTTTCCTACATTGCATTTATACTTGTATGGAATACTGCTTCCTTAATTCGGTCCTTTTTGGTTTTGGAGAATGTTTAATGCCCAACATCGAACATAATTCTCACGACAAAAGAGGCAATTGGAAAGGGGAGCAATTTCTGCACCCCATTGCCAATTCTCCTGTGGTTGGATTCTCTAGAACCACAAGAATCTTATCACTACACCTTAATTAATGAGTTTTTAAAATCCAAAATGCCATCGGTATAAAAGATGCAACATGGGGAGATAAAAGGAAAAAGGGGATGGGATTTCACTCGCTTTGGGCATAGTCTCCCTGGAGGGTTTATTTGTTGAATGAGAATTTGAGAACTCTGTTTTCGTTTAATGGGTTATAAGAACTATGTGTATTCTGGATATGTAACTGTTGTGGGAATTAAGTAGTCAAGATGAAGTAAAAATGTGCCTGTAGTCCTCCCTTTGCTTTAACTTTGTGGATAGTACATTGGTCAATCACACAATTTAGTAGGCAAAGCTTTCCTGTAGACATTTAAAATTTCCATTTCTGATACGATCTCTATAAGAACCAACAATGTTAGTACAAAATGACCCCGTTTCTGTGTCATAGTTTATTGATTTATCCATTCTATTTCTATGTACTGCAGTTCCTTGTGTGTGTGATGACTGATAGTATCTATTATCCTTTTACAGGAAGAAGATGTTGACATAATTATGCACCACCTACTTGGCTTAATCAATCCATTTTTTAGTAGGTAATAACCTTCAGTTGTTGCTTTCTAGATTTTGATCAAATAAAAATGTCACCACATACCTATCAGTTCTTTTATAACTATGGTGTCAAGACCCACGCTATGTATTGTGCTCCTTACACACCCCACTAATCTCTAGGAGGCTTCTCTCACCCTAAGGCTAAAGACTATAAAGGTGCTAAATTATTAATTTTTTAACCTAAATTTGAACCGAGGAACTTGGAGAATATCTTCCAAACACCCCAAGAGCTTATCACCACACACTTATCATTACATTCTCCTTTTTCTTTTAAGCCTTTTGTTGTGGAATAAAAATGTTTGAGCTTATCACCACAGTTGAGAATATTTACTTTCATTCCTCTCTAACTATGACTTATCCACATGAACGTAAATATGCACGACTTGATTACTGATTACACTCTGAAACCTGATTCTTCTTCTTTGATTTTTCTTGAAGAAATGAACAAAAGTATCAAACAGAATCTCCTGAAGTGAAGCAGGAAAAGTTCAGCAGCATGGTCCACGATGCAGCAAAGCCGTTTCTGTCAGCAAGAGCAGACCGATTCGTGCATGAGCTGGAACTGTTTCTTGCTTCAGGTTTGAACATCGAAGCCTACGATTCGGTTTACTTGCAACGGTTGGGCTGGAGTGAGCCAGTGGTGCCTAGTGTGGCTATCAAAGAAGATCTTGGACTCAAACCTGTAACTCCATACTTGTACATCTTTGACTATGACCCTGATGATGGTGAA

mRNA sequence

ATGGAATTGCAGAGCACTGCGCAAATGGAGATGGAGACGGCTAGTTCTTCTTACGACGATACGAACCCCTGCCCGATCTGTCTCGGATCCATAATTCGACCTTCTTACCTCGACAAATGCTTCCATAAGTTTTGTTACAATTGCATTGTGCAATGGACCAAAGTGGTTTCTGGCAAGCGCTCCTGCATTCTTTCGTCCATTAAGTGTCCTTTATGCAAGACAGAAAGTTCTTCTATAATACATGGATTAGATGGACATTGTTTCCAAAGGCATTACGTTAATCAGGATTTTCAGGATAGCTTTATCTTATCAAAAGCCCACAAGTATAGATTACAGTGCTATTATACTGAACCAGGTTTCTTGGAGGACATATTCAATGTACAAAGATACTGGAAATTACGAAAGTATCTCCAAGCAAACCAATGGCTTGAAGTTTGGTTGAAAAGGGAACTTCAAGCTTTGATTCAGGAAGAAGATGTTGACATAATTATGCACCACCTACTTGGCTTAATCAATCCATTTTTTAGTAGAAATGAACAAAAGTATCAAACAGAATCTCCTGAAGTGAAGCAGGAAAAGTTCAGCAGCATGGTCCACGATGCAGCAAAGCCGTTTCTGTCAGCAAGAGCAGACCGATTCGTGCATGAGCTGGAACTGTTTCTTGCTTCAGGTTTGAACATCGAAGCCTACGATTCGGTTTACTTGCAACGGTTGGGCTGGAGTGAGCCAGTGGTGCCTAGTGTGGCTATCAAAGAAGATCTTGGACTCAAACCTGTAACTCCATACTTGTACATCTTTGACTATGACCCTGATGATGGTGAA

Coding sequence (CDS)

ATGGAATTGCAGAGCACTGCGCAAATGGAGATGGAGACGGCTAGTTCTTCTTACGACGATACGAACCCCTGCCCGATCTGTCTCGGATCCATAATTCGACCTTCTTACCTCGACAAATGCTTCCATAAGTTTTGTTACAATTGCATTGTGCAATGGACCAAAGTGGTTTCTGGCAAGCGCTCCTGCATTCTTTCGTCCATTAAGTGTCCTTTATGCAAGACAGAAAGTTCTTCTATAATACATGGATTAGATGGACATTGTTTCCAAAGGCATTACGTTAATCAGGATTTTCAGGATAGCTTTATCTTATCAAAAGCCCACAAGTATAGATTACAGTGCTATTATACTGAACCAGGTTTCTTGGAGGACATATTCAATGTACAAAGATACTGGAAATTACGAAAGTATCTCCAAGCAAACCAATGGCTTGAAGTTTGGTTGAAAAGGGAACTTCAAGCTTTGATTCAGGAAGAAGATGTTGACATAATTATGCACCACCTACTTGGCTTAATCAATCCATTTTTTAGTAGAAATGAACAAAAGTATCAAACAGAATCTCCTGAAGTGAAGCAGGAAAAGTTCAGCAGCATGGTCCACGATGCAGCAAAGCCGTTTCTGTCAGCAAGAGCAGACCGATTCGTGCATGAGCTGGAACTGTTTCTTGCTTCAGGTTTGAACATCGAAGCCTACGATTCGGTTTACTTGCAACGGTTGGGCTGGAGTGAGCCAGTGGTGCCTAGTGTGGCTATCAAAGAAGATCTTGGACTCAAACCTGTAACTCCATACTTGTACATCTTTGACTATGACCCTGATGATGGTGAA

Protein sequence

MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKRSCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGFLEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQKYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGWSEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE

Homology

[Close]

BLAST of MS007334 vs. NCBI nr
Match: XP_022150768.1 (uncharacterized protein LOC111018833 [Momordica charantia])

HSP 1 Score: 565.8 bits (1457), Expect = 2.0e-157
Identity = 271/274 (98.91%), Postives = 272/274 (99.27%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQSTAQ EMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR
Sbjct: 6   MELQSTAQKEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 65

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SCILS IKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF
Sbjct: 66  SCILSPIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 125

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ
Sbjct: 126 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 185

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           KYQTESPEVKQEKFSS+VHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 186 KYQTESPEVKQEKFSSIVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 245

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 275
           SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE
Sbjct: 246 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 279

[Close]

BLAST of MS007334 vs. NCBI nr
Match: XP_038889020.1 (uncharacterized protein LOC120078783 isoform X1 [Benincasa hispida])

HSP 1 Score: 506.1 bits (1302), Expect = 1.8e-139
Identity = 240/272 (88.24%), Postives = 256/272 (94.12%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQSTA +E  T+SS  DDTNPCPICLG II+PSYLDKCFHKFCYNCI+QWTKVVSGK 
Sbjct: 6   MELQSTAPVETATSSSFSDDTNPCPICLGLIIQPSYLDKCFHKFCYNCILQWTKVVSGKH 65

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SC+LSSIKCPLCKTESSSIIHGLDGHC QRHYVN+DFQDSFILSKAHKYRLQCYYTEPGF
Sbjct: 66  SCMLSSIKCPLCKTESSSIIHGLDGHCLQRHYVNRDFQDSFILSKAHKYRLQCYYTEPGF 125

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           L+DIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEED+DIIMHHLLG IN FFSRNE 
Sbjct: 126 LDDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDIDIIMHHLLGFINSFFSRNEP 185

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           +YQTE+PE+K+E F+ MV DAAKPFLSARADRFV ELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 186 RYQTETPELKRETFNRMVLDAAKPFLSARADRFVLELELFLASGLNIEAYDSVYLQRLGW 245

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDD 273
           +EPVVP +AIKEDLGLKPVTPYLYIF+YDPDD
Sbjct: 246 NEPVVPIMAIKEDLGLKPVTPYLYIFEYDPDD 277

[Close]

BLAST of MS007334 vs. NCBI nr
Match: XP_022999126.1 (uncharacterized protein LOC111493604 [Cucurbita maxima])

HSP 1 Score: 498.0 bits (1281), Expect = 5.0e-137
Identity = 235/274 (85.77%), Postives = 257/274 (93.80%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQ TA +EMET+SSS DDTNPCPICLG II+PSYLDKCFHKFCYNCIVQWTKVVSGK 
Sbjct: 1   MELQGTAPLEMETSSSSSDDTNPCPICLGPIIQPSYLDKCFHKFCYNCIVQWTKVVSGKH 60

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SC LSSIKCPLCKTES SIIHGLDGHCFQRHYVNQDFQDSFILSKA KYRLQCYYTEPGF
Sbjct: 61  SCTLSSIKCPLCKTESPSIIHGLDGHCFQRHYVNQDFQDSFILSKAQKYRLQCYYTEPGF 120

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           L+DIFNVQ+YWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLG I+ FFSRNE 
Sbjct: 121 LDDIFNVQKYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGSIHSFFSRNEH 180

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           +YQTE+P++K+++F+SM+ DAAKPFLS RADRFV ELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 181 RYQTETPQLKRDRFNSMILDAAKPFLSERADRFVLELELFLASGLNIEAYDSVYLQRLGW 240

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 275
           ++P  PS+A+ EDLGL+PVTPYL+IFDYDPDDG+
Sbjct: 241 NKPFGPSMAV-EDLGLEPVTPYLFIFDYDPDDGD 273

[Close]

BLAST of MS007334 vs. NCBI nr
Match: XP_022946760.1 (uncharacterized protein LOC111450733 [Cucurbita moschata])

HSP 1 Score: 496.1 bits (1276), Expect = 1.9e-136
Identity = 235/274 (85.77%), Postives = 257/274 (93.80%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQ TA +EMET+SSS DDTNPCPICLG II+PSYLDKCFHKFCYNCIVQWTKVVSGK 
Sbjct: 1   MELQGTAPLEMETSSSSSDDTNPCPICLGPIIQPSYLDKCFHKFCYNCIVQWTKVVSGKH 60

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SC LSSIKCPLCKTES SIIHGLDGHCFQRHYVNQDFQDSFILSKA KYRLQCYYTEPGF
Sbjct: 61  SCTLSSIKCPLCKTESPSIIHGLDGHCFQRHYVNQDFQDSFILSKAQKYRLQCYYTEPGF 120

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           L+DIF+VQ+YWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLG I+ FFSRNE 
Sbjct: 121 LDDIFSVQKYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGSIHSFFSRNEP 180

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           +YQTE+P++K+E+F+SM+ DAAKPFLS RADRFV ELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 181 RYQTETPQLKRERFNSMILDAAKPFLSERADRFVLELELFLASGLNIEAYDSVYLQRLGW 240

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 275
           ++PV PS+A+ EDLG +PVTPYL+IFDYDPDDG+
Sbjct: 241 NKPVGPSMAV-EDLGHEPVTPYLFIFDYDPDDGD 273

[Close]

BLAST of MS007334 vs. NCBI nr
Match: XP_023545827.1 (uncharacterized protein LOC111805146 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 495.0 bits (1273), Expect = 4.3e-136
Identity = 234/274 (85.40%), Postives = 257/274 (93.80%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQ TA +EMET+SSS DDTNPCPICLG II+PSYLDKCFHKFCYNCIVQWTKVVSGK 
Sbjct: 1   MELQGTAPLEMETSSSSSDDTNPCPICLGPIIQPSYLDKCFHKFCYNCIVQWTKVVSGKH 60

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SC LSSIKCPLCKTES SIIHGLDGHCFQRHYVNQDFQDSFILSKA KYRLQCYYTEPGF
Sbjct: 61  SCTLSSIKCPLCKTESPSIIHGLDGHCFQRHYVNQDFQDSFILSKAQKYRLQCYYTEPGF 120

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           L+DIF+VQ+YWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLG I+ FFSRNE 
Sbjct: 121 LDDIFSVQKYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGSIHSFFSRNEP 180

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           +YQTE+P++K+++F+SM+ DAAKPFLS RADRFV ELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 181 RYQTETPQLKRDRFNSMILDAAKPFLSERADRFVLELELFLASGLNIEAYDSVYLQRLGW 240

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 275
           ++PV PS+A+ EDLG +PVTPYL+IFDYDPDDG+
Sbjct: 241 NKPVGPSMAV-EDLGHEPVTPYLFIFDYDPDDGD 273

[Close]

BLAST of MS007334 vs. ExPASy Swiss-Prot
Match: Q80Z37 (E3 ubiquitin-protein ligase Topors OS=Mus musculus OX=10090 GN=Topors PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 8.4e-10
Identity = 31/106 (29.25%), Postives = 50/106 (47.17%), Query Frame = 0


Query: 12  ETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKRSCILSSIKCPL 71
           +T  +     + CPICL      SYLD+C HKFC+ C+ +W+K          +  +CPL
Sbjct: 92  QTVPADASPDSKCPICLDRFDNVSYLDRCLHKFCFRCVQEWSK----------NKAECPL 151

Query: 72  CKTESSSIIHGLDGH-CFQRHYVNQDFQDSFILSKAHKYRLQCYYT 117
           CK    SI H +     F+ + +   +  SF   +  ++R +   T
Sbjct: 152 CKQPFDSIFHSVRAEDDFKEYVLRPSYNGSFTNPEVRRFRYRTTMT 187

[Close]

BLAST of MS007334 vs. ExPASy Swiss-Prot
Match: Q9NS56 (E3 ubiquitin-protein ligase Topors OS=Homo sapiens OX=9606 GN=TOPORS PE=1 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.9e-09
Identity = 31/101 (30.69%), Postives = 49/101 (48.51%), Query Frame = 0


Query: 12  ETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKRSCILSSIKCPL 71
           +T  +     + CPICL      SYLD+C HKFC+ C+ +W+K          +  +CPL
Sbjct: 91  QTVPADASPDSKCPICLDRFDNVSYLDRCLHKFCFRCVQEWSK----------NKAECPL 150

Query: 72  CKTESSSIIHGLDGH-CFQRHYVNQDFQDSFIL-SKAHKYR 111
           CK    SI H +     F+ + +   +  SF+   +  +YR
Sbjct: 151 CKQPFDSIFHSVRAEDDFKEYVLRPSYNGSFVTPDRRFRYR 181

[Close]

BLAST of MS007334 vs. ExPASy Swiss-Prot
Match: P29129 (E3 ubiquitin-protein ligase ICP0 OS=Suid herpesvirus 1 (strain Indiana-Funkhauser / Becker) OX=31523 GN=EP0 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 9.6e-06
Identity = 24/61 (39.34%), Postives = 31/61 (50.82%), Query Frame = 0


Query: 24 CPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKRSCILSSIKCPLCKTESSSIIHGL 83
          CPICL      +    C HKFC +CI +WT          L+S  CPLC    +SI+H +
Sbjct: 46 CPICLDVAATEAQTLPCMHKFCLDCIQRWT----------LTSTACPLCNARVTSILHHV 96

Query: 84 D 85
          D
Sbjct: 106 D 96

[Close]

BLAST of MS007334 vs. ExPASy TrEMBL
Match: A0A6J1DAB4 (uncharacterized protein LOC111018833 OS=Momordica charantia OX=3673 GN=LOC111018833 PE=4 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 9.5e-158
Identity = 271/274 (98.91%), Postives = 272/274 (99.27%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQSTAQ EMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR
Sbjct: 6   MELQSTAQKEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 65

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SCILS IKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF
Sbjct: 66  SCILSPIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 125

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ
Sbjct: 126 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 185

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           KYQTESPEVKQEKFSS+VHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 186 KYQTESPEVKQEKFSSIVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 245

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 275
           SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE
Sbjct: 246 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 279

[Close]

BLAST of MS007334 vs. ExPASy TrEMBL
Match: A0A6J1KC78 (uncharacterized protein LOC111493604 OS=Cucurbita maxima OX=3661 GN=LOC111493604 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.4e-137
Identity = 235/274 (85.77%), Postives = 257/274 (93.80%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQ TA +EMET+SSS DDTNPCPICLG II+PSYLDKCFHKFCYNCIVQWTKVVSGK 
Sbjct: 1   MELQGTAPLEMETSSSSSDDTNPCPICLGPIIQPSYLDKCFHKFCYNCIVQWTKVVSGKH 60

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SC LSSIKCPLCKTES SIIHGLDGHCFQRHYVNQDFQDSFILSKA KYRLQCYYTEPGF
Sbjct: 61  SCTLSSIKCPLCKTESPSIIHGLDGHCFQRHYVNQDFQDSFILSKAQKYRLQCYYTEPGF 120

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           L+DIFNVQ+YWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLG I+ FFSRNE 
Sbjct: 121 LDDIFNVQKYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGSIHSFFSRNEH 180

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           +YQTE+P++K+++F+SM+ DAAKPFLS RADRFV ELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 181 RYQTETPQLKRDRFNSMILDAAKPFLSERADRFVLELELFLASGLNIEAYDSVYLQRLGW 240

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 275
           ++P  PS+A+ EDLGL+PVTPYL+IFDYDPDDG+
Sbjct: 241 NKPFGPSMAV-EDLGLEPVTPYLFIFDYDPDDGD 273

[Close]

BLAST of MS007334 vs. ExPASy TrEMBL
Match: A0A6J1G4Z2 (uncharacterized protein LOC111450733 OS=Cucurbita moschata OX=3662 GN=LOC111450733 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 9.3e-137
Identity = 235/274 (85.77%), Postives = 257/274 (93.80%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQ TA +EMET+SSS DDTNPCPICLG II+PSYLDKCFHKFCYNCIVQWTKVVSGK 
Sbjct: 1   MELQGTAPLEMETSSSSSDDTNPCPICLGPIIQPSYLDKCFHKFCYNCIVQWTKVVSGKH 60

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SC LSSIKCPLCKTES SIIHGLDGHCFQRHYVNQDFQDSFILSKA KYRLQCYYTEPGF
Sbjct: 61  SCTLSSIKCPLCKTESPSIIHGLDGHCFQRHYVNQDFQDSFILSKAQKYRLQCYYTEPGF 120

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           L+DIF+VQ+YWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLG I+ FFSRNE 
Sbjct: 121 LDDIFSVQKYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGSIHSFFSRNEP 180

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           +YQTE+P++K+E+F+SM+ DAAKPFLS RADRFV ELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 181 RYQTETPQLKRERFNSMILDAAKPFLSERADRFVLELELFLASGLNIEAYDSVYLQRLGW 240

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 275
           ++PV PS+A+ EDLG +PVTPYL+IFDYDPDDG+
Sbjct: 241 NKPVGPSMAV-EDLGHEPVTPYLFIFDYDPDDGD 273

[Close]

BLAST of MS007334 vs. ExPASy TrEMBL
Match: A0A0A0LLN5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G021620 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.6e-134
Identity = 232/274 (84.67%), Postives = 251/274 (91.61%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQSTA MEMET +S+ D TNPCPICLG I + SYLDKCFH FCYNCIVQWTKVVSGKR
Sbjct: 6   MELQSTASMEMETRTSTSDYTNPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKR 65

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SC LSSIKCPLCKTESSSIIHGLDGH FQRHYVN DFQDSFILSKAH+YRLQCYYTEPGF
Sbjct: 66  SCTLSSIKCPLCKTESSSIIHGLDGHNFQRHYVNPDFQDSFILSKAHRYRLQCYYTEPGF 125

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           L DIF+VQRYWKL+KYLQANQWLEVWLKRELQALIQEEDVDIIMHH LGLIN FF RNE 
Sbjct: 126 LNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFLGLINSFFRRNEP 185

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           +YQTE+PE+K+++FS  + DAAKPFLSARADRF+ ELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 186 EYQTETPELKRKRFSQTILDAAKPFLSARADRFILELELFLASGLNIEAYDSVYLQRLGW 245

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDDGE 275
           ++P+VPSVA +EDLGLK VTPYLYIFD DPDDG+
Sbjct: 246 NKPIVPSVANEEDLGLKSVTPYLYIFDCDPDDGD 279

[Close]

BLAST of MS007334 vs. ExPASy TrEMBL
Match: A0A5D3D454 (RING zinc finger protein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00310 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.2e-130
Identity = 228/272 (83.82%), Postives = 248/272 (91.18%), Query Frame = 0


Query: 1   MELQSTAQMEMETASSSYDDTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKR 60
           MELQS A ME++T++S+ D T+PCPICLG I + SYLDKCFH FCYNCIVQWTKVVSGKR
Sbjct: 6   MELQSAASMEIKTSTSTSDYTDPCPICLGPINQSSYLDKCFHNFCYNCIVQWTKVVSGKR 65

Query: 61  SCILSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGF 120
           SC LSSIKCPLCKTESSSIIHGLDGH FQRHYVN DFQDSFILSKAHKYRLQCYYTEPGF
Sbjct: 66  SCRLSSIKCPLCKTESSSIIHGLDGHHFQRHYVNPDFQDSFILSKAHKYRLQCYYTEPGF 125

Query: 121 LEDIFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ 180
           L DIF+VQRYWKL+KYLQANQWLEVWLKRELQALIQEEDVDIIMHH +GLIN FF RNE 
Sbjct: 126 LNDIFDVQRYWKLQKYLQANQWLEVWLKRELQALIQEEDVDIIMHHFVGLINSFFRRNEP 185

Query: 181 KYQTESPEVKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 240
           KYQTE+PE+K+++FS  + DAAKPFLSARADRF+ ELELFLASGLNIEAYDSVYLQRLGW
Sbjct: 186 KYQTETPELKRKRFSQTILDAAKPFLSARADRFMLELELFLASGLNIEAYDSVYLQRLGW 245

Query: 241 SEPVVPSVAIKEDLGLKPVTPYLYIFDYDPDD 273
           +EP+V SVA +EDLGLK VTPYLYIFD DPDD
Sbjct: 246 NEPIVLSVANEEDLGLKSVTPYLYIFDNDPDD 277

[Close]

BLAST of MS007334 vs. TAIR 10
Match: AT3G05250.1 (RING/U-box superfamily protein )

HSP 1 Score: 298.9 bits (764), Expect = 4.2e-81
Identity = 151/277 (54.51%), Postives = 195/277 (70.40%), Query Frame = 0


Query: 7   AQMEMETASSSYD---DTNPCPICLGSIIRPSYLDKCFHKFCYNCIVQWTKVVSGKRSCI 66
           A  E+   S S D   D +PCPICLG  +R SYLD CFHKFC+NCI QW KVVS K S  
Sbjct: 2   AAKEIAGGSGSIDDGSDFDPCPICLGQFLRESYLDTCFHKFCFNCIKQWIKVVSSKASKQ 61

Query: 67  LSSIKCPLCKTESSSIIHGLDGHCFQRHYVNQDFQDSFILSKAHKYRLQCYYTEPGFLED 126
            SS+ CPLCKTE+ SIIH  DG  F+RHY++ +  D F+L+K  +YRLQCYYTE GFL D
Sbjct: 62  RSSVTCPLCKTENLSIIHNYDGCSFERHYIDPNIPDGFVLTKEQRYRLQCYYTESGFLAD 121

Query: 127 IFNVQRYWKLRKYLQANQWLEVWLKRELQALIQEEDVDIIMHHLLGLINPFFSRNEQ--K 186
           +F+V R+WKL+K+LQ N+ LE WL+RELQAL+QEEDVDI++HHL+G++  F  R +Q  K
Sbjct: 122 VFDVARFWKLQKFLQPNRCLEAWLRRELQALMQEEDVDIVLHHLVGVMESFCKRIKQRRK 181

Query: 187 YQTESPE-VKQEKFSSMVHDAAKPFLSARADRFVHELELFLASGLNIEAYDSVYLQRLGW 246
            +T S E   QE+F ++V +AA+PF+ AR DRFV ELELFLA+GLN+EAYD++Y Q L  
Sbjct: 182 QETRSAETTNQEQFKAVVSEAARPFVMARTDRFVDELELFLAAGLNLEAYDAIYKQGLEG 241

Query: 247 SEPVVPSVAIKEDLGLKP------VTPYLYIFDYDPD 272
           +       A +E   ++       VTPYL+IF+ D D
Sbjct: 242 NNRREIGAASEEREEVEEHNVRTRVTPYLFIFEEDSD 278

[Close]

BLAST of MS007334 vs. TAIR 10
Match: AT2G39100.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-08
Identity = 64/266 (24.06%), Postives = 102/266 (38.35%), Query Frame = 0


Query: 24  CPICLGSIIR---PSYLDKCFHKFCYNCIVQWTKVVSGKRSCILSSIKCPLCKTESSS-- 83
           CPICL ++      + +  C H +C  CI +W+   S KR+       CPLC T   S  
Sbjct: 39  CPICLENLTERRSAAVITVCKHGYCLACIRKWS---SFKRN-------CPLCNTRFDSWF 98

Query: 84  IIHGL--------------DGHCFQRHYVNQDFQDSFI----------LSKAHKYRLQCY 143
           I+                 D      H  N   +   I           S++     +  
Sbjct: 99  IVSDFASRKYHKEQLPILRDRETLTYHRNNPSDRRRIIQRSRDVLENSSSRSRPLPWRRS 158

Query: 144 YTEPGFLED--IFNVQRYWKLRKY-----------------LQANQW--------LEVWL 203
           +  PG + D  IF  +  W+   Y                 L  N +        +E W+
Sbjct: 159 FGRPGSVPDSVIFQRKLQWRASIYTKQLRAVRLHSRRLELSLAVNDYTKAKITERIEPWI 218

Query: 204 KRELQALIQEEDVDIIMHHLLGLINPFFSRNEQKYQTESPEVKQEKFSSMVHDAAKPFLS 234
           +RELQA++ + D  +I+H    L      R   +   ++  + +++ SS+     + FLS
Sbjct: 219 RRELQAVLGDPDPSVIVHFASALFIKRLERENNRQTGQTGMLVEDEVSSL-----RKFLS 278

[Close]

BLAST of MS007334 vs. TAIR 10
Match: AT3G05670.1 (RING/U-box protein )

HSP 1 Score: 42.7 bits (99), Expect = 5.4e-04
Identity = 23/61 (37.70%), Postives = 29/61 (47.54%), Query Frame = 0


Query: 22  NPCPICLGSIIR---PSYLDKCFHKFCYNCIVQWTKVVSGKRSCILSSIKCPLCKTESSS 80
           N C ICL           LD C H FC+ CI++W+KV S          +CPLCK    +
Sbjct: 411 NVCGICLSEEDMRRLKGTLDCCSHYFCFTCIMEWSKVES----------RCPLCKQRFRT 461

The following BLAST results are available for this feature:

BLAST of MS007334 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150768.1	2.0e-157	98.91	uncharacterized protein LOC111018833 [Momordica charantia]	[more]
XP_038889020.1	1.8e-139	88.24	uncharacterized protein LOC120078783 isoform X1 [Benincasa hispida]	[more]
XP_022999126.1	5.0e-137	85.77	uncharacterized protein LOC111493604 [Cucurbita maxima]	[more]
XP_022946760.1	1.9e-136	85.77	uncharacterized protein LOC111450733 [Cucurbita moschata]	[more]
XP_023545827.1	4.3e-136	85.40	uncharacterized protein LOC111805146 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS007334 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q80Z37	8.4e-10	29.25	E3 ubiquitin-protein ligase Topors OS=Mus musculus OX=10090 GN=Topors PE=1 SV=1	[more]
Q9NS56	1.9e-09	30.69	E3 ubiquitin-protein ligase Topors OS=Homo sapiens OX=9606 GN=TOPORS PE=1 SV=1	[more]
P29129	9.6e-06	39.34	E3 ubiquitin-protein ligase ICP0 OS=Suid herpesvirus 1 (strain Indiana-Funkhause...	[more]

BLAST of MS007334 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAB4	9.5e-158	98.91	uncharacterized protein LOC111018833 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]
A0A6J1KC78	2.4e-137	85.77	uncharacterized protein LOC111493604 OS=Cucurbita maxima OX=3661 GN=LOC111493604...	[more]
A0A6J1G4Z2	9.3e-137	85.77	uncharacterized protein LOC111450733 OS=Cucurbita moschata OX=3662 GN=LOC1114507...	[more]
A0A0A0LLN5	5.6e-134	84.67	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G021620 P...	[more]
A0A5D3D454	2.2e-130	83.82	RING zinc finger protein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of MS007334 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05250.1	4.2e-81	54.51	RING/U-box superfamily protein	[more]
AT2G39100.1	1.2e-08	24.06	RING/U-box superfamily protein	[more]
AT3G05670.1	5.4e-04	37.70	RING/U-box protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 24..72 e-value: 1.0E-4 score: 31.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 24..73 score: 11.781134
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 3..101 e-value: 1.0E-11 score: 46.1
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 24..72 e-value: 2.5E-8 score: 33.7
None	No IPR available	PANTHER	PTHR47692	RING/U-BOX SUPERFAMILY PROTEIN	coord: 11..272
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 20..80
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 40..49

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS007334.1	MS007334.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding