MS007305 (gene) Bitter gourd (TR) v1

Overview
NameMS007305
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPDZ domain-containing protein
Locationscaffold25: 2139097 .. 2142326 (+)
RNA-Seq ExpressionMS007305
SyntenyMS007305
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGGTTTCGAAATCGCATAACACACTCATACGGATCGCGGCTTTTGCTGCTGCTGGTTCTTGTTTCTTGTACGGCAGAAGCGAATTAGATTCGAGTGAGTTCTTGAGCTCTTTGATTGTTTGTCGAGAAATGGATCAACATAGCTGAATAGTTTGGGTGTTATTTTTCCGTTTTTCTGCCGAGTTCACACTGTAGTTGAACGTAAATGTTCAATTAGTTAACTTCTTGAAAATGTCTACTCATTTGTCTGCACACTGTCATCCATTCGCTTAGTTTTTTGGGTGTTTTCCACGTTTCTTCATTGAAGGACCCACGGTAGTATTGTCGATTCCTGCTTCTTGGAGCGAACCACTGTTTCTTCCATGCCAGACCACGCAGGGCTTAAAGGTTTCGTCGATTCCGGCGTTTGATCACCGGGGATTGGGTATCTCTCTTGATTGCAAGTTAGTTTTCTATTTCATTTCTGTTTTTACTGGAAATTTGCTTGATGTGTGAAATTGTAGGCCTTCCGTTGTTCGCTTCAAGAGTCGGTCCTGCTCCACCATCTGATGCGGAAAAGGAAACGCCTGGAGTTGCTGGAGATGGCCAGAAGCCTTGTCCACAATGTTTGGGAAGAGATACAATTGCTAATGCAGCAGCAAATGTCGGCCCTGCTGTTGTAAATATATCTGTTCCTCATGGTATTTGAGTTTGCAATTTTCATGTTATTTTTCCCATGGTTTTCCTGAAGAAAAAAAGAAGTCGTAAGAGTTTGTTTCCTTAGGTATTTATGGAATTGCTGCTGCCAAAAGCATGGGCTCCGGAACTATTATTGACAAGGATGGTACAATTTTAACATGTGCCCATGTTGTGACTGGTTTTTACAGTTCACGAGCTGTATCGAAAGGAAAGGTAAGCAGTTCTATAGCTTGTTAGTTAGCTTTCTGAATTTAATAAGAATATGAATAGCGTTGTTTTGTTGTTTTGTAGAAATATTGATATGTTTATATTGCTTGGATTTAGTAAATCTTTTTTTACTTTATGCCAATTTTTGAAAATTAGATTAATTTTCTTCCATGGTCAATACTATGTTCTGTTTGTTACGATAATTTCATGTTAGTTTATGTTTGTACATAGTTTGCAATTATGTTTTTGACAGAACTTTTCCAATGGCGAATGTCTTTTTAGCTCTTCAGACCATTGATAAGCCACAAATGTCTTCATAATTCTCTGTTGAACCTTCATATTTCGTTCAATTAAGTTTCAATCTTAGAACTGCTGAATGCTTGCGCGTTATTTTTTTAAAAATTATTTTATATTTGAACTGACATAGGTAGAGGTTACTTTACAAGATGGTCGGACGTTTGAAGGTACAGTAATGAATGCTGATTTTCACTCTGATATTGCCATCGTAAAAATAAATTCTAAAAGCCCTCTTCCCATGGCAAAACTTGGTTCTTCAAGCAAGCTTCGACCTGGGGATTGGGTTGTAGCAATTGGGTGTCCACTTTCACTTCAGAATACTGTCACAGCTGGTATAGTAAGGTAGCCAAATGGTGAAAGTTTCTCTGTCCTTGATATTTTATTTCGATGGAAGAGAGAAAAACATAAACTCTGTTTATAAACTTTTTTCACATCGTTGTTAGTGAACATATGAATGAAACATCCTCCTTTTCAGGATGGCGTGCTTAAGATTAACTGTTATCTATACCTACTGAACAATATAGGTCTACATCTACTCGCCGAAATTAGAAGGCGAAGAATCAAAATTAACAGGGTAGCTGATTAGAGTTTATGCAGGTTTTTAGTAATATTTGGTTGTCTACTTTTCTTCTTTGATCCAGTTGTGTTGACCGTAAGAGCAGTGATTTGGGTCTTGGTGGAATACGAAGGGAATATCTGCAAACAGATTGTGCAATTAATGTGGTATGTATCACTTTCTTTTTCTACCGCATCTCACCTTGCAATAACCTATATTCCTTATTGTGGTAGAAGTTTGTGAACTTATTATTCTAGCCTTATTGTACACAAAATGTTGGCATTCTGTAATCTATATTTGTCGTGTAGGGAAATTCTGGGGGCCCTCTTGTTAATATCGATGGTGAAGTTGTTGGTGTGAACATTATGAAAGTGGATGATGCTGTTGGATTAAGTTTTGCTGTACCAATTGATTCAGTCTCCAAAATTACAGAGCAATTCAAGAAAAGAGGGTAAACTGAATTTAGAGACTATAGTATGGTTGCTAGTGAATTGGATTGAATTGAAGACTTCATTTGTATGTAGAAGAGATCTAGTTCACTCTCATTTTTCCTCTTGCATTCAGTTTAAGGTTTGAGTGAAATGATATCAATGACATCTAGTAAAATCCTCCTAATTTTGAAGTATCCTCAATGTTTCTGTATCATTGCTTTAATTCTTTTTGCCCTTTATTTTGTCTTAGTTGTTTGCTTGAATCTTCTAATAGAGTGGTGGAGATTCATTTGTCTGAATTGAATTCGTTGAGATGTTTTCTGTGATCTAGAATGTAATATGATTTAGTTGTGAATTAGTTGAGAAATTGCATTAAACCTGGTGATTTCATCTACAAAATGTGGGCGGCTCTCTCTCTCTCTCTCTTTCTATCTCCATCTATAAAATTGATTCTCAATTTTGTTTGCCAATAGGAACTAGAATATGCTTTACTTCCTGATCTATTTTTTGACAGATGATGATAATGTAGTGTAATGACGCAGGAGAGTTATTCGGCCTTGGCTCGGATTGAAAATGATTGATCTCAATGAAATGATCATCGAACAACTTAAAGAAAGAGATACCACTTTTCCAGCTGTCACTGAAGGGGTTCTTATAGCTATGGTAATATTACAATCTGATCTAGGAGTACCGACGTGTCTTTGCGTTTAATAAATTTGGGACGAGTGAATAGATTCTTCAAATGCTTCAATAGGTAACTCCTGGGTCCCCTGCTAGTCGTGCTGGATTTCGTTCGGGTGATGTTGTCATTGAGCTCGATAGCCATCCTGTTGCAAGTATCAAAGAGGTATTTGAACCTTCTTAGTTCCCATTAGTAGTATTGTGTTTTAGATCATTTTCTACTTATACATATCAGTGAAGCTCAGTGTTTAATTTTGTTGCAGATCATCGAAATTATGGGAGATAGAGTTGGGGTTCCATTAAAGGCAGTTGTGAAGAGATCAGTTAATAGCACCATCACTTTGACTGTTCTTCCGGAGGAGTCCAATCCAGATATG

mRNA sequence

AAAAAGGTTTCGAAATCGCATAACACACTCATACGGATCGCGGCTTTTGCTGCTGCTGGTTCTTGTTTCTTGTACGGCAGAAGCGAATTAGATTCGAGACCCACGGTAGTATTGTCGATTCCTGCTTCTTGGAGCGAACCACTGTTTCTTCCATGCCAGACCACGCAGGGCTTAAAGGTTTCGTCGATTCCGGCGTTTGATCACCGGGGATTGGGTATCTCTCTTGATTGCAAGTTAGAAACGCCTGGAGTTGCTGGAGATGGCCAGAAGCCTTGTCCACAATGTTTGGGAAGAGATACAATTGCTAATGCAGCAGCAAATGTCGGCCCTGCTGTTGTAAATATATCTTTTGTTTCCTTAGGTATTTATGGAATTGCTGCTGCCAAAAGCATGGGCTCCGGAACTATTATTGACAAGGATGGTACAATTTTAACATGTGCCCATGTTGTGACTGGTTTTTACAGTTCACGAGCTGTATCGAAAGGAAAGGTAGAGGTTACTTTACAAGATGGTCGGACGTTTGAAGGTACAGTAATGAATGCTGATTTTCACTCTGATATTGCCATCGTAAAAATAAATTCTAAAAGCCCTCTTCCCATGGCAAAACTTGGTTCTTCAAGCAAGCTTCGACCTGGGGATTGGGTTGTAGCAATTGGGTGTCCACTTTCACTTCAGAATACTGTCACAGCTGGTATAGTAAGTTGTGTTGACCGTAAGAGCAGTGATTTGGGTCTTGGTGGAATACGAAGGGAATATCTGCAAACAGATTGTGCAATTAATGTGGGAAATTCTGGGGGCCCTCTTGTTAATATCGATGGTGAAGTTGTTGGTGTGAACATTATGAAAGTGGATGATGCTGTTGGATTAAGTTTTGCTGTACCAATTGATTCAGTCTCCAAAATTACAGAGCAATTCAAGAAAAGAGGGAGAGTTATTCGGCCTTGGCTCGGATTGAAAATGATTGATCTCAATGAAATGATCATCGAACAACTTAAAGAAAGAGATACCACTTTTCCAGCTGTCACTGAAGGGGTTCTTATAGCTATGGTAACTCCTGGGTCCCCTGCTAGTCGTGCTGGATTTCGTTCGGGTGATGTTGTCATTGAGCTCGATAGCCATCCTGTTGCAAGTATCAAAGAGATCATCGAAATTATGGGAGATAGAGTTGGGGTTCCATTAAAGGCAGTTGTGAAGAGATCAGTTAATAGCACCATCACTTTGACTGTTCTTCCGGAGGAGTCCAATCCAGATATG

Coding sequence (CDS)

AAAAAGGTTTCGAAATCGCATAACACACTCATACGGATCGCGGCTTTTGCTGCTGCTGGTTCTTGTTTCTTGTACGGCAGAAGCGAATTAGATTCGAGACCCACGGTAGTATTGTCGATTCCTGCTTCTTGGAGCGAACCACTGTTTCTTCCATGCCAGACCACGCAGGGCTTAAAGGTTTCGTCGATTCCGGCGTTTGATCACCGGGGATTGGGTATCTCTCTTGATTGCAAGTTAGAAACGCCTGGAGTTGCTGGAGATGGCCAGAAGCCTTGTCCACAATGTTTGGGAAGAGATACAATTGCTAATGCAGCAGCAAATGTCGGCCCTGCTGTTGTAAATATATCTTTTGTTTCCTTAGGTATTTATGGAATTGCTGCTGCCAAAAGCATGGGCTCCGGAACTATTATTGACAAGGATGGTACAATTTTAACATGTGCCCATGTTGTGACTGGTTTTTACAGTTCACGAGCTGTATCGAAAGGAAAGGTAGAGGTTACTTTACAAGATGGTCGGACGTTTGAAGGTACAGTAATGAATGCTGATTTTCACTCTGATATTGCCATCGTAAAAATAAATTCTAAAAGCCCTCTTCCCATGGCAAAACTTGGTTCTTCAAGCAAGCTTCGACCTGGGGATTGGGTTGTAGCAATTGGGTGTCCACTTTCACTTCAGAATACTGTCACAGCTGGTATAGTAAGTTGTGTTGACCGTAAGAGCAGTGATTTGGGTCTTGGTGGAATACGAAGGGAATATCTGCAAACAGATTGTGCAATTAATGTGGGAAATTCTGGGGGCCCTCTTGTTAATATCGATGGTGAAGTTGTTGGTGTGAACATTATGAAAGTGGATGATGCTGTTGGATTAAGTTTTGCTGTACCAATTGATTCAGTCTCCAAAATTACAGAGCAATTCAAGAAAAGAGGGAGAGTTATTCGGCCTTGGCTCGGATTGAAAATGATTGATCTCAATGAAATGATCATCGAACAACTTAAAGAAAGAGATACCACTTTTCCAGCTGTCACTGAAGGGGTTCTTATAGCTATGGTAACTCCTGGGTCCCCTGCTAGTCGTGCTGGATTTCGTTCGGGTGATGTTGTCATTGAGCTCGATAGCCATCCTGTTGCAAGTATCAAAGAGATCATCGAAATTATGGGAGATAGAGTTGGGGTTCCATTAAAGGCAGTTGTGAAGAGATCAGTTAATAGCACCATCACTTTGACTGTTCTTCCGGAGGAGTCCAATCCAGATATG

Protein sequence

KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKVSSIPAFDHRGLGISLDCKLETPGVAGDGQKPCPQCLGRDTIANAAANVGPAVVNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPGSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPEESNPDM
Homology
BLAST of MS007305 vs. NCBI nr
Match: XP_022150785.1 (putative protease Do-like 14 [Momordica charantia])

HSP 1 Score: 786.6 bits (2030), Expect = 1.1e-223
Identity = 408/429 (95.10%), Postives = 410/429 (95.57%), Query Frame = 0

Query: 1   KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKV 60
           KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTV LSIPASWSEPLFLPCQTTQGLKV
Sbjct: 6   KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVALSIPASWSEPLFLPCQTTQGLKV 65

Query: 61  SSIPAFDHRGLGISL-----------DCKLETPGVAGDGQKPCPQCLGRDTIANAAANVG 120
           SSIPAFDHRGLG+ L           D + ETPGVAGDGQKPCPQCLGRDTIANAAANVG
Sbjct: 66  SSIPAFDHRGLGLPLFASRVGPAPPSDAEKETPGVAGDGQKPCPQCLGRDTIANAAANVG 125

Query: 121 PAVVNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQ 180
           PAVVNIS V  GIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQ
Sbjct: 126 PAVVNIS-VPHGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQ 185

Query: 181 DGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVT 240
           DGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVT
Sbjct: 186 DGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVT 245

Query: 241 AGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGL 300
           AGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGL
Sbjct: 246 AGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGL 305

Query: 301 SFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAM 360
           SFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAM
Sbjct: 306 SFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAM 365

Query: 361 VTPGSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTV 419
           VTPGSPASRAGFRSGDVVIELDSH VASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTV
Sbjct: 366 VTPGSPASRAGFRSGDVVIELDSHRVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTV 425

BLAST of MS007305 vs. NCBI nr
Match: XP_022965762.1 (putative protease Do-like 14 isoform X1 [Cucurbita maxima])

HSP 1 Score: 679.1 bits (1751), Expect = 2.4e-191
Identity = 357/425 (84.00%), Postives = 375/425 (88.24%), Query Frame = 0

Query: 2   KVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKVS 61
           KVS S NTL RIAA AAAGSCF Y  S+LDS  +VVLSIPA+ SEPLFLP QT  G  + 
Sbjct: 8   KVSSSRNTLGRIAAIAAAGSCFWYAGSKLDSGSSVVLSIPAALSEPLFLPWQTAHGFTIH 67

Query: 62  SIPAFDHRGLGISLDCKLETP----GV----AGDGQKPCPQCLGRDTIANAAANVGPAVV 121
              AFDH+ LG+S      +P    GV     GD QKPCP+CL RDTIANAAA+VGPAVV
Sbjct: 68  PSGAFDHQKLGLSFCSSRVSPAPPSGVEKEKPGDTQKPCPRCLDRDTIANAAADVGPAVV 127

Query: 122 NISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRT 181
           NIS VS GIYGIA AKSMGSGTIIDKDGTILTCAHVVT F+  RA SKGKVEVTLQDGRT
Sbjct: 128 NIS-VSHGIYGIATAKSMGSGTIIDKDGTILTCAHVVTDFHGPRAASKGKVEVTLQDGRT 187

Query: 182 FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV 241
           FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV
Sbjct: 188 FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV 247

Query: 242 SCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAV 301
           SCVDRKSSDLGLGG+RREYLQTDCAINVGNSGGPLVN+DGEV+GVNIMKVDDAVGLSFAV
Sbjct: 248 SCVDRKSSDLGLGGMRREYLQTDCAINVGNSGGPLVNVDGEVIGVNIMKVDDAVGLSFAV 307

Query: 302 PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPG 361
           PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDT+FP VT+GVL+AMVTPG
Sbjct: 308 PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTSFPDVTKGVLVAMVTPG 367

Query: 362 SPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPEE 419
           SPASRAGFR GDVVIE D  PV SI+EIIEIMGDRVGVPLKAVVKRS+NSTITLTVLPEE
Sbjct: 368 SPASRAGFRPGDVVIEFDKQPVGSIQEIIEIMGDRVGVPLKAVVKRSLNSTITLTVLPEE 427

BLAST of MS007305 vs. NCBI nr
Match: XP_022965763.1 (putative protease Do-like 14 isoform X2 [Cucurbita maxima])

HSP 1 Score: 679.1 bits (1751), Expect = 2.4e-191
Identity = 357/425 (84.00%), Postives = 375/425 (88.24%), Query Frame = 0

Query: 2   KVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKVS 61
           KVS S NTL RIAA AAAGSCF Y  S+LDS  +VVLSIPA+ SEPLFLP QT  G  + 
Sbjct: 7   KVSSSRNTLGRIAAIAAAGSCFWYAGSKLDSGSSVVLSIPAALSEPLFLPWQTAHGFTIH 66

Query: 62  SIPAFDHRGLGISLDCKLETP----GV----AGDGQKPCPQCLGRDTIANAAANVGPAVV 121
              AFDH+ LG+S      +P    GV     GD QKPCP+CL RDTIANAAA+VGPAVV
Sbjct: 67  PSGAFDHQKLGLSFCSSRVSPAPPSGVEKEKPGDTQKPCPRCLDRDTIANAAADVGPAVV 126

Query: 122 NISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRT 181
           NIS VS GIYGIA AKSMGSGTIIDKDGTILTCAHVVT F+  RA SKGKVEVTLQDGRT
Sbjct: 127 NIS-VSHGIYGIATAKSMGSGTIIDKDGTILTCAHVVTDFHGPRAASKGKVEVTLQDGRT 186

Query: 182 FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV 241
           FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV
Sbjct: 187 FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV 246

Query: 242 SCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAV 301
           SCVDRKSSDLGLGG+RREYLQTDCAINVGNSGGPLVN+DGEV+GVNIMKVDDAVGLSFAV
Sbjct: 247 SCVDRKSSDLGLGGMRREYLQTDCAINVGNSGGPLVNVDGEVIGVNIMKVDDAVGLSFAV 306

Query: 302 PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPG 361
           PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDT+FP VT+GVL+AMVTPG
Sbjct: 307 PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTSFPDVTKGVLVAMVTPG 366

Query: 362 SPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPEE 419
           SPASRAGFR GDVVIE D  PV SI+EIIEIMGDRVGVPLKAVVKRS+NSTITLTVLPEE
Sbjct: 367 SPASRAGFRPGDVVIEFDKQPVGSIQEIIEIMGDRVGVPLKAVVKRSLNSTITLTVLPEE 426

BLAST of MS007305 vs. NCBI nr
Match: KAG6586251.1 (putative protease Do-like 14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 675.2 bits (1741), Expect = 3.5e-190
Identity = 353/426 (82.86%), Postives = 375/426 (88.03%), Query Frame = 0

Query: 1   KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKV 60
           +KVS S NTL RIAA AAAGSCF Y  S+LD+  +VVLSIPA+ SEPLFLP QTT G  +
Sbjct: 7   RKVSSSRNTLGRIAAIAAAGSCFWYAGSKLDNGSSVVLSIPAALSEPLFLPWQTTHGFTI 66

Query: 61  SSIPAFDHRGLGISLDCKLETP----GV----AGDGQKPCPQCLGRDTIANAAANVGPAV 120
               AFDH+ LG+S      +P    GV     GD QKPCP+CL RDTIANAAA+VGPAV
Sbjct: 67  HPSGAFDHQKLGLSFCSSRVSPAPPSGVEKEKPGDTQKPCPRCLDRDTIANAAADVGPAV 126

Query: 121 VNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGR 180
           VNIS VS GIYGIA AKSMGSGTIIDKDGTILTCAHVVT F+  RA SKGKVEVTLQDGR
Sbjct: 127 VNIS-VSHGIYGIATAKSMGSGTIIDKDGTILTCAHVVTDFHGPRAASKGKVEVTLQDGR 186

Query: 181 TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI 240
           TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI
Sbjct: 187 TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI 246

Query: 241 VSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFA 300
           VSCVDRKSSDLGLGG+RREYLQTDCAINVGNSGGPLVN+DGEV+GVNIMKVDDAVGLSFA
Sbjct: 247 VSCVDRKSSDLGLGGMRREYLQTDCAINVGNSGGPLVNVDGEVIGVNIMKVDDAVGLSFA 306

Query: 301 VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTP 360
           VPIDS+SKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERD +FP VT+GVL+AMVTP
Sbjct: 307 VPIDSISKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDASFPDVTKGVLVAMVTP 366

Query: 361 GSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPE 419
           GSPASRAGFR GDVVIE D  PV SI+EIIEIMGDRVG+PLKAVVKRS+NS ITLTVLPE
Sbjct: 367 GSPASRAGFRPGDVVIEFDKQPVGSIQEIIEIMGDRVGIPLKAVVKRSLNSIITLTVLPE 426

BLAST of MS007305 vs. NCBI nr
Match: XP_022938300.1 (putative protease Do-like 14 isoform X1 [Cucurbita moschata])

HSP 1 Score: 672.2 bits (1733), Expect = 3.0e-189
Identity = 352/426 (82.63%), Postives = 373/426 (87.56%), Query Frame = 0

Query: 1   KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKV 60
           +KVS S NTL RIAA AAAGSCF Y  S+LD+  +VVLSIPA+ SEPLFLP QT  G  +
Sbjct: 7   RKVSSSRNTLGRIAAIAAAGSCFWYAGSKLDNGSSVVLSIPAALSEPLFLPWQTAHGFTI 66

Query: 61  SSIPAFDHRGLGISLDCKLETP----GV----AGDGQKPCPQCLGRDTIANAAANVGPAV 120
               AFDH+ LG+S      +P    GV     GD QKPCP+CL RDTIANAAA+VGPAV
Sbjct: 67  HPSGAFDHQKLGLSFCSSRVSPAPPSGVEKEKPGDTQKPCPRCLDRDTIANAAADVGPAV 126

Query: 121 VNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGR 180
           VNIS VS GIYGIA AKSMGSGTIIDKDGTILTCAHVVT F+  RA SKGKVEVTLQDGR
Sbjct: 127 VNIS-VSHGIYGIATAKSMGSGTIIDKDGTILTCAHVVTDFHGPRAASKGKVEVTLQDGR 186

Query: 181 TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI 240
           TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI
Sbjct: 187 TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI 246

Query: 241 VSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFA 300
           VSCVDRKSSDLGLGG+RREYLQTDCAINVGNSGGPLVN+DGEV+GVNIMKVDDAVGLSFA
Sbjct: 247 VSCVDRKSSDLGLGGMRREYLQTDCAINVGNSGGPLVNVDGEVIGVNIMKVDDAVGLSFA 306

Query: 301 VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTP 360
           VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERD +FP VT+GVL+AMVTP
Sbjct: 307 VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDASFPDVTKGVLVAMVTP 366

Query: 361 GSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPE 419
           GSPASRAGFR GDVVIE D  PV SI+EIIEIMGDRVG+PLKAVVKRS+N  ITLTVLPE
Sbjct: 367 GSPASRAGFRPGDVVIEFDKQPVGSIQEIIEIMGDRVGIPLKAVVKRSLNGIITLTVLPE 426

BLAST of MS007305 vs. ExPASy Swiss-Prot
Match: Q3E6S8 (Putative protease Do-like 14 OS=Arabidopsis thaliana OX=3702 GN=DEGP14 PE=3 SV=2)

HSP 1 Score: 475.3 bits (1222), Expect = 7.0e-133
Identity = 261/435 (60.00%), Postives = 302/435 (69.43%), Query Frame = 0

Query: 4   SKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPL-FLPCQTTQGL---- 63
           S   + LIRI + A A S  LY  +  D+R  V L+IP S  E L  LP Q + GL    
Sbjct: 11  SSKRSELIRIISVATATSGILYASTNPDARTRVSLAIPESVRESLSLLPWQISPGLIHRP 70

Query: 64  ---------------KVSSIPAFDHRGLGISLDCKLETPGVAGDGQKPCPQCLGRDTIAN 123
                            S  P  D +G+ +           +    KP    LGRDTIAN
Sbjct: 71  EQSLFGNFVFSSRVSPKSEAPINDEKGVSVE---------ASDSSSKPSNGYLGRDTIAN 130

Query: 124 AAANVGPAVVNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGK 183
           AAA +GPAVVN+S V  G +GI+  KS+GSGTIID DGTILTCAHVV  F + R  SKG+
Sbjct: 131 AAARIGPAVVNLS-VPQGFHGISMGKSIGSGTIIDADGTILTCAHVVVDFQNIRHSSKGR 190

Query: 184 VEVTLQDGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLS 243
           V+VTLQDGRTFEG V+NAD  SDIA+VKI SK+PLP AKLG SSKLRPGDWV+A+GCPLS
Sbjct: 191 VDVTLQDGRTFEGVVVNADLQSDIALVKIKSKTPLPTAKLGFSSKLRPGDWVIAVGCPLS 250

Query: 244 LQNTVTAGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKV 303
           LQNTVTAGIVSCVDRKSSDLGLGG  REYLQTDC+IN GNSGGPLVN+DGEV+GVNIMKV
Sbjct: 251 LQNTVTAGIVSCVDRKSSDLGLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVNIMKV 310

Query: 304 DDAVGLSFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTE 363
             A GL F+VPIDSVSKI E FKK GRVIRPW+GLKM++LN +I+ QLKERD  FP V  
Sbjct: 311 LAADGLGFSVPIDSVSKIIEHFKKSGRVIRPWIGLKMVELNNLIVAQLKERDPMFPDVER 370

Query: 364 GVLIAMVTPGSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNS 419
           GVL+  V PGSPA RAGF+ GDVV+  D  PV      IEIM DRVG  ++ VV+RS   
Sbjct: 371 GVLVPTVIPGSPADRAGFKPGDVVVRFDGKPV------IEIMDDRVGKRMQVVVERSNKE 429

BLAST of MS007305 vs. ExPASy Swiss-Prot
Match: A9JRB3 (Serine protease HTRA1B OS=Danio rerio OX=7955 GN=htra1b PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 8.7e-59
Identity = 130/341 (38.12%), Postives = 197/341 (57.77%), Query Frame = 0

Query: 83  GVAGDGQKPCPQCLGR--DTIANAAANVGPAVVNISFVSLGIYGIAAAKSMGSGTIIDKD 142
           G  G G +  P  L    + IA+    + PAVV+I      ++    A + GSG ++ +D
Sbjct: 149 GACGKGHEENPDSLRHRYNFIADVVEKIAPAVVHIELFRKNVFNREVAVASGSGFVVSED 208

Query: 143 GTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRTFEGTVMNADFHSDIAIVKINSKSPLPM 202
           G I+T AHVV         +K +V+V L+ G T++  + + D  +DIA++KI++   LP+
Sbjct: 209 GLIVTNAHVV--------ANKHRVKVELKTGTTYDAKIKDVDEKADIALIKIDAPMKLPV 268

Query: 203 AKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIVSCVDRKSSDLGLGGIRREYLQTDCAIN 262
             LG S+ LRPG++VVAIG P SLQNTVT GIVS   R   +LGL     +Y+QTD  IN
Sbjct: 269 LLLGRSADLRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAIIN 328

Query: 263 VGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAVPIDSVSKI-----TEQFKKRGRVIRPW 322
            GNSGGPLVN+DGEV+G+N +KV    G+SFA+P D + +        Q K +    + +
Sbjct: 329 YGNSGGPLVNLDGEVIGINTLKV--TAGISFAIPSDKIRQFLAESHDRQAKGKTATKKKY 388

Query: 323 LGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPGSPASRAGFRSGDVVIELDSHPV 382
           +G++M+ L   + ++LK+R   FP VT G  +  V P +PA   G +  DV+I ++   +
Sbjct: 389 IGVRMMTLTPTLAKELKQRKNDFPDVTSGAYVIEVIPKTPAEVGGLKESDVIISINGQRI 448

Query: 383 ASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPEESNP 417
            S  ++   +  +    L+AVV+R  N  I LT++PEE +P
Sbjct: 449 TSASDVSTAI--KTDESLRAVVRRG-NEDIILTIIPEEIDP 476

BLAST of MS007305 vs. ExPASy Swiss-Prot
Match: Q9D236 (Serine protease HTRA3 OS=Mus musculus OX=10090 GN=Htra3 PE=1 SV=3)

HSP 1 Score: 228.4 bits (581), Expect = 1.5e-58
Identity = 125/317 (39.43%), Postives = 196/317 (61.83%), Query Frame = 0

Query: 101 IANAAANVGPAVVNIS-FVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAV 160
           IA+    + PAVV+I  F+   ++G     S GSG I+ + G I+T AHVV+   SS A 
Sbjct: 149 IADVVEKIAPAVVHIELFLRHPLFGRNVPLSSGSGFIMSEAGLIVTNAHVVSS--SSTAS 208

Query: 161 SKGKVEVTLQDGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIG 220
            + +++V LQ+G  +E T+ + D  SDIA + I+ K  LP+  LG S+ LRPG++VVAIG
Sbjct: 209 GRQQLKVQLQNGDAYEATIQDIDKKSDIATIVIHPKKKLPVLLLGHSADLRPGEFVVAIG 268

Query: 221 CPLSLQNTVTAGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVN 280
            P +LQNTVT GIVS   R   +LGL     +Y+QTD  IN GNSGGPLVN+DGEV+G+N
Sbjct: 269 SPFALQNTVTTGIVSTAQRDGKELGLRDSDMDYIQTDAIINYGNSGGPLVNLDGEVIGIN 328

Query: 281 IMKVDDAVGLSFAVPIDSVSKITEQFKKRGRVIRPW----LGLKMIDLNEMIIEQLKERD 340
            +KV  A G+SFA+P D +++   +F+ +   ++ W    +G++M  +   ++E+LK  +
Sbjct: 329 TLKV--AAGISFAIPSDRITRFLSEFQNKH--VKDWKKRFIGIRMRTITPSLVEELKAAN 388

Query: 341 TTFPAVTEGVLIAMVTPGSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKA 400
             FPAV+ G+ +  V P SP+ R G + GD++++++  P+A   E+ E + +   + L+ 
Sbjct: 389 PDFPAVSSGIYVQEVVPNSPSQRGGIQDGDIIVKVNGRPLADSSELQEAVLNESSLLLEV 448

Query: 401 VVKRSVNSTITLTVLPE 413
              R  N  +  +++PE
Sbjct: 449 ---RRGNDDLLFSIIPE 456

BLAST of MS007305 vs. ExPASy Swiss-Prot
Match: F1N152 (Serine protease HTRA1 OS=Bos taurus OX=9913 GN=HTRA1 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.5e-58
Identity = 139/342 (40.64%), Postives = 203/342 (59.36%), Query Frame = 0

Query: 83  GVAGDGQKPCPQCLGR--DTIANAAANVGPAVVNIS-FVSLGIYGIAAAKSMGSGTIIDK 142
           G  G GQ+  P  L    + IA+    + PAVV+I  F  L         + GSG I+ +
Sbjct: 160 GACGQGQED-PNSLRHKYNFIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSE 219

Query: 143 DGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRTFEGTVMNADFHSDIAIVKINSKSPLP 202
           DG I+T AHVVT        +K +V+V L++G T+E  + + D  +DIA++KI+ +  LP
Sbjct: 220 DGLIVTNAHVVT--------NKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLP 279

Query: 203 MAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIVSCVDRKSSDLGLGGIRREYLQTDCAI 262
           +  LG SS+LRPG++VVAIG P SLQNTVT GIVS   R   +LGL     +Y+QTD  I
Sbjct: 280 VLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAII 339

Query: 263 NVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAVPIDSVSK-ITEQFKK--RGRVI--RP 322
           N GNSGGPLVN+DGEV+G+N +KV    G+SFA+P D + K +TE   +  +G+ I  + 
Sbjct: 340 NYGNSGGPLVNLDGEVIGINTLKV--TAGISFAIPSDKIKKFLTESHDRQAKGKAITKKK 399

Query: 323 WLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPGSPASRAGFRSGDVVIELDSHP 382
           ++G++M+ L     ++LK+R   FP V  G  I  V P +PA   G +  DV+I ++   
Sbjct: 400 YIGIRMMSLTPSKAKELKDRHRDFPDVLSGAYIIEVIPDTPAEAGGLKENDVIISINGQS 459

Query: 383 VASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPEESNP 417
           V S  ++ +++  +    L  VV+R  N  I +TV+PEE +P
Sbjct: 460 VVSANDVSDVI--KKESTLNMVVRRG-NEDIMITVIPEEIDP 487

BLAST of MS007305 vs. ExPASy Swiss-Prot
Match: Q92743 (Serine protease HTRA1 OS=Homo sapiens OX=9606 GN=HTRA1 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.5e-58
Identity = 139/342 (40.64%), Postives = 203/342 (59.36%), Query Frame = 0

Query: 83  GVAGDGQKPCPQCLGR--DTIANAAANVGPAVVNIS-FVSLGIYGIAAAKSMGSGTIIDK 142
           G  G GQ+  P  L    + IA+    + PAVV+I  F  L         + GSG I+ +
Sbjct: 153 GACGQGQED-PNSLRHKYNFIADVVEKIAPAVVHIELFRKLPFSKREVPVASGSGFIVSE 212

Query: 143 DGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRTFEGTVMNADFHSDIAIVKINSKSPLP 202
           DG I+T AHVVT        +K +V+V L++G T+E  + + D  +DIA++KI+ +  LP
Sbjct: 213 DGLIVTNAHVVT--------NKHRVKVELKNGATYEAKIKDVDEKADIALIKIDHQGKLP 272

Query: 203 MAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIVSCVDRKSSDLGLGGIRREYLQTDCAI 262
           +  LG SS+LRPG++VVAIG P SLQNTVT GIVS   R   +LGL     +Y+QTD  I
Sbjct: 273 VLLLGRSSELRPGEFVVAIGSPFSLQNTVTTGIVSTTQRGGKELGLRNSDMDYIQTDAII 332

Query: 263 NVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAVPIDSVSK-ITEQFKK--RGRVI--RP 322
           N GNSGGPLVN+DGEV+G+N +KV    G+SFA+P D + K +TE   +  +G+ I  + 
Sbjct: 333 NYGNSGGPLVNLDGEVIGINTLKV--TAGISFAIPSDKIKKFLTESHDRQAKGKAITKKK 392

Query: 323 WLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPGSPASRAGFRSGDVVIELDSHP 382
           ++G++M+ L     ++LK+R   FP V  G  I  V P +PA   G +  DV+I ++   
Sbjct: 393 YIGIRMMSLTSSKAKELKDRHRDFPDVISGAYIIEVIPDTPAEAGGLKENDVIISINGQS 452

Query: 383 VASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPEESNP 417
           V S  ++ +++  +    L  VV+R  N  I +TV+PEE +P
Sbjct: 453 VVSANDVSDVI--KRESTLNMVVRRG-NEDIMITVIPEEIDP 480

BLAST of MS007305 vs. ExPASy TrEMBL
Match: A0A6J1D9G4 (putative protease Do-like 14 OS=Momordica charantia OX=3673 GN=LOC111018849 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.2e-224
Identity = 408/429 (95.10%), Postives = 410/429 (95.57%), Query Frame = 0

Query: 1   KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKV 60
           KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTV LSIPASWSEPLFLPCQTTQGLKV
Sbjct: 6   KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVALSIPASWSEPLFLPCQTTQGLKV 65

Query: 61  SSIPAFDHRGLGISL-----------DCKLETPGVAGDGQKPCPQCLGRDTIANAAANVG 120
           SSIPAFDHRGLG+ L           D + ETPGVAGDGQKPCPQCLGRDTIANAAANVG
Sbjct: 66  SSIPAFDHRGLGLPLFASRVGPAPPSDAEKETPGVAGDGQKPCPQCLGRDTIANAAANVG 125

Query: 121 PAVVNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQ 180
           PAVVNIS V  GIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQ
Sbjct: 126 PAVVNIS-VPHGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQ 185

Query: 181 DGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVT 240
           DGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVT
Sbjct: 186 DGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVT 245

Query: 241 AGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGL 300
           AGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGL
Sbjct: 246 AGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGL 305

Query: 301 SFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAM 360
           SFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAM
Sbjct: 306 SFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAM 365

Query: 361 VTPGSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTV 419
           VTPGSPASRAGFRSGDVVIELDSH VASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTV
Sbjct: 366 VTPGSPASRAGFRSGDVVIELDSHRVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTV 425

BLAST of MS007305 vs. ExPASy TrEMBL
Match: A0A6J1HPX7 (putative protease Do-like 14 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465556 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.2e-191
Identity = 357/425 (84.00%), Postives = 375/425 (88.24%), Query Frame = 0

Query: 2   KVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKVS 61
           KVS S NTL RIAA AAAGSCF Y  S+LDS  +VVLSIPA+ SEPLFLP QT  G  + 
Sbjct: 7   KVSSSRNTLGRIAAIAAAGSCFWYAGSKLDSGSSVVLSIPAALSEPLFLPWQTAHGFTIH 66

Query: 62  SIPAFDHRGLGISLDCKLETP----GV----AGDGQKPCPQCLGRDTIANAAANVGPAVV 121
              AFDH+ LG+S      +P    GV     GD QKPCP+CL RDTIANAAA+VGPAVV
Sbjct: 67  PSGAFDHQKLGLSFCSSRVSPAPPSGVEKEKPGDTQKPCPRCLDRDTIANAAADVGPAVV 126

Query: 122 NISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRT 181
           NIS VS GIYGIA AKSMGSGTIIDKDGTILTCAHVVT F+  RA SKGKVEVTLQDGRT
Sbjct: 127 NIS-VSHGIYGIATAKSMGSGTIIDKDGTILTCAHVVTDFHGPRAASKGKVEVTLQDGRT 186

Query: 182 FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV 241
           FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV
Sbjct: 187 FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV 246

Query: 242 SCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAV 301
           SCVDRKSSDLGLGG+RREYLQTDCAINVGNSGGPLVN+DGEV+GVNIMKVDDAVGLSFAV
Sbjct: 247 SCVDRKSSDLGLGGMRREYLQTDCAINVGNSGGPLVNVDGEVIGVNIMKVDDAVGLSFAV 306

Query: 302 PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPG 361
           PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDT+FP VT+GVL+AMVTPG
Sbjct: 307 PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTSFPDVTKGVLVAMVTPG 366

Query: 362 SPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPEE 419
           SPASRAGFR GDVVIE D  PV SI+EIIEIMGDRVGVPLKAVVKRS+NSTITLTVLPEE
Sbjct: 367 SPASRAGFRPGDVVIEFDKQPVGSIQEIIEIMGDRVGVPLKAVVKRSLNSTITLTVLPEE 426

BLAST of MS007305 vs. ExPASy TrEMBL
Match: A0A6J1HL69 (putative protease Do-like 14 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465556 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.2e-191
Identity = 357/425 (84.00%), Postives = 375/425 (88.24%), Query Frame = 0

Query: 2   KVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKVS 61
           KVS S NTL RIAA AAAGSCF Y  S+LDS  +VVLSIPA+ SEPLFLP QT  G  + 
Sbjct: 8   KVSSSRNTLGRIAAIAAAGSCFWYAGSKLDSGSSVVLSIPAALSEPLFLPWQTAHGFTIH 67

Query: 62  SIPAFDHRGLGISLDCKLETP----GV----AGDGQKPCPQCLGRDTIANAAANVGPAVV 121
              AFDH+ LG+S      +P    GV     GD QKPCP+CL RDTIANAAA+VGPAVV
Sbjct: 68  PSGAFDHQKLGLSFCSSRVSPAPPSGVEKEKPGDTQKPCPRCLDRDTIANAAADVGPAVV 127

Query: 122 NISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRT 181
           NIS VS GIYGIA AKSMGSGTIIDKDGTILTCAHVVT F+  RA SKGKVEVTLQDGRT
Sbjct: 128 NIS-VSHGIYGIATAKSMGSGTIIDKDGTILTCAHVVTDFHGPRAASKGKVEVTLQDGRT 187

Query: 182 FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV 241
           FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV
Sbjct: 188 FEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIV 247

Query: 242 SCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAV 301
           SCVDRKSSDLGLGG+RREYLQTDCAINVGNSGGPLVN+DGEV+GVNIMKVDDAVGLSFAV
Sbjct: 248 SCVDRKSSDLGLGGMRREYLQTDCAINVGNSGGPLVNVDGEVIGVNIMKVDDAVGLSFAV 307

Query: 302 PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPG 361
           PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDT+FP VT+GVL+AMVTPG
Sbjct: 308 PIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTSFPDVTKGVLVAMVTPG 367

Query: 362 SPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPEE 419
           SPASRAGFR GDVVIE D  PV SI+EIIEIMGDRVGVPLKAVVKRS+NSTITLTVLPEE
Sbjct: 368 SPASRAGFRPGDVVIEFDKQPVGSIQEIIEIMGDRVGVPLKAVVKRSLNSTITLTVLPEE 427

BLAST of MS007305 vs. ExPASy TrEMBL
Match: A0A6J1FCS0 (putative protease Do-like 14 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444435 PE=3 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.4e-189
Identity = 352/426 (82.63%), Postives = 373/426 (87.56%), Query Frame = 0

Query: 1   KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKV 60
           +KVS S NTL RIAA AAAGSCF Y  S+LD+  +VVLSIPA+ SEPLFLP QT  G  +
Sbjct: 6   RKVSSSRNTLGRIAAIAAAGSCFWYAGSKLDNGSSVVLSIPAALSEPLFLPWQTAHGFTI 65

Query: 61  SSIPAFDHRGLGISLDCKLETP----GV----AGDGQKPCPQCLGRDTIANAAANVGPAV 120
               AFDH+ LG+S      +P    GV     GD QKPCP+CL RDTIANAAA+VGPAV
Sbjct: 66  HPSGAFDHQKLGLSFCSSRVSPAPPSGVEKEKPGDTQKPCPRCLDRDTIANAAADVGPAV 125

Query: 121 VNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGR 180
           VNIS VS GIYGIA AKSMGSGTIIDKDGTILTCAHVVT F+  RA SKGKVEVTLQDGR
Sbjct: 126 VNIS-VSHGIYGIATAKSMGSGTIIDKDGTILTCAHVVTDFHGPRAASKGKVEVTLQDGR 185

Query: 181 TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI 240
           TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI
Sbjct: 186 TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI 245

Query: 241 VSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFA 300
           VSCVDRKSSDLGLGG+RREYLQTDCAINVGNSGGPLVN+DGEV+GVNIMKVDDAVGLSFA
Sbjct: 246 VSCVDRKSSDLGLGGMRREYLQTDCAINVGNSGGPLVNVDGEVIGVNIMKVDDAVGLSFA 305

Query: 301 VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTP 360
           VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERD +FP VT+GVL+AMVTP
Sbjct: 306 VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDASFPDVTKGVLVAMVTP 365

Query: 361 GSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPE 419
           GSPASRAGFR GDVVIE D  PV SI+EIIEIMGDRVG+PLKAVVKRS+N  ITLTVLPE
Sbjct: 366 GSPASRAGFRPGDVVIEFDKQPVGSIQEIIEIMGDRVGIPLKAVVKRSLNGIITLTVLPE 425

BLAST of MS007305 vs. ExPASy TrEMBL
Match: A0A6J1FJD3 (putative protease Do-like 14 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444435 PE=3 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.4e-189
Identity = 352/426 (82.63%), Postives = 373/426 (87.56%), Query Frame = 0

Query: 1   KKVSKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPLFLPCQTTQGLKV 60
           +KVS S NTL RIAA AAAGSCF Y  S+LD+  +VVLSIPA+ SEPLFLP QT  G  +
Sbjct: 7   RKVSSSRNTLGRIAAIAAAGSCFWYAGSKLDNGSSVVLSIPAALSEPLFLPWQTAHGFTI 66

Query: 61  SSIPAFDHRGLGISLDCKLETP----GV----AGDGQKPCPQCLGRDTIANAAANVGPAV 120
               AFDH+ LG+S      +P    GV     GD QKPCP+CL RDTIANAAA+VGPAV
Sbjct: 67  HPSGAFDHQKLGLSFCSSRVSPAPPSGVEKEKPGDTQKPCPRCLDRDTIANAAADVGPAV 126

Query: 121 VNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGR 180
           VNIS VS GIYGIA AKSMGSGTIIDKDGTILTCAHVVT F+  RA SKGKVEVTLQDGR
Sbjct: 127 VNIS-VSHGIYGIATAKSMGSGTIIDKDGTILTCAHVVTDFHGPRAASKGKVEVTLQDGR 186

Query: 181 TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI 240
           TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI
Sbjct: 187 TFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGI 246

Query: 241 VSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFA 300
           VSCVDRKSSDLGLGG+RREYLQTDCAINVGNSGGPLVN+DGEV+GVNIMKVDDAVGLSFA
Sbjct: 247 VSCVDRKSSDLGLGGMRREYLQTDCAINVGNSGGPLVNVDGEVIGVNIMKVDDAVGLSFA 306

Query: 301 VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTP 360
           VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERD +FP VT+GVL+AMVTP
Sbjct: 307 VPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDASFPDVTKGVLVAMVTP 366

Query: 361 GSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVGVPLKAVVKRSVNSTITLTVLPE 419
           GSPASRAGFR GDVVIE D  PV SI+EIIEIMGDRVG+PLKAVVKRS+N  ITLTVLPE
Sbjct: 367 GSPASRAGFRPGDVVIEFDKQPVGSIQEIIEIMGDRVGIPLKAVVKRSLNGIITLTVLPE 426

BLAST of MS007305 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 449.1 bits (1154), Expect = 3.8e-126
Identity = 247/407 (60.69%), Postives = 282/407 (69.29%), Query Frame = 0

Query: 4   SKSHNTLIRIAAFAAAGSCFLYGRSELDSRPTVVLSIPASWSEPL-FLPCQTTQGL---- 63
           S   + LIRI + A A S  LY  +  D+R  V L+IP S  E L  LP Q + GL    
Sbjct: 11  SSKRSELIRIISVATATSGILYASTNPDARTRVSLAIPESVRESLSLLPWQISPGLIHRP 70

Query: 64  ---------------KVSSIPAFDHRGLGISLDCKLETPGVAGDGQKPCPQCLGRDTIAN 123
                            S  P  D +G+ +           +    KP    LGRDTIAN
Sbjct: 71  EQSLFGNFVFSSRVSPKSEAPINDEKGVSVE---------ASDSSSKPSNGYLGRDTIAN 130

Query: 124 AAANVGPAVVNISFVSLGIYGIAAAKSMGSGTIIDKDGTILTCAHVVTGFYSSRAVSKGK 183
           AAA +GPAVVN+S V  G +GI+  KS+GSGTIID DGTILTCAHVV  F + R  SKG+
Sbjct: 131 AAARIGPAVVNLS-VPQGFHGISMGKSIGSGTIIDADGTILTCAHVVVDFQNIRHSSKGR 190

Query: 184 VEVTLQDGRTFEGTVMNADFHSDIAIVKINSKSPLPMAKLGSSSKLRPGDWVVAIGCPLS 243
           V+VTLQDGRTFEG V+NAD  SDIA+VKI SK+PLP AKLG SSKLRPGDWV+A+GCPLS
Sbjct: 191 VDVTLQDGRTFEGVVVNADLQSDIALVKIKSKTPLPTAKLGFSSKLRPGDWVIAVGCPLS 250

Query: 244 LQNTVTAGIVSCVDRKSSDLGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKV 303
           LQNTVTAGIVSCVDRKSSDLGLGG  REYLQTDC+IN GNSGGPLVN+DGEV+GVNIMKV
Sbjct: 251 LQNTVTAGIVSCVDRKSSDLGLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVNIMKV 310

Query: 304 DDAVGLSFAVPIDSVSKITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTE 363
             A GL F+VPIDSVSKI E FKK GRVIRPW+GLKM++LN +I+ QLKERD  FP V  
Sbjct: 311 LAADGLGFSVPIDSVSKIIEHFKKSGRVIRPWIGLKMVELNNLIVAQLKERDPMFPDVER 370

Query: 364 GVLIAMVTPGSPASRAGFRSGDVVIELDSHPVASIKEIIEIMGDRVG 391
           GVL+  V PGSPA RAGF+ GDVV+  D  PV      IEIM DRVG
Sbjct: 371 GVLVPTVIPGSPADRAGFKPGDVVVRFDGKPV------IEIMDDRVG 401

BLAST of MS007305 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 136.0 bits (341), Expect = 7.1e-32
Identity = 98/304 (32.24%), Postives = 156/304 (51.32%), Query Frame = 0

Query: 132 GSGTIIDKDGTILTCAHVVTGFYSSRAVSKGKVEVTLQDGRTFEGTVMNADFHSDIAIVK 191
           GSG + DK G I+T  HV+ G           + VTL D  TF+  V+  D   D+A+++
Sbjct: 157 GSGFVWDKQGHIVTNYHVIRG--------ASDLRVTLADQTTFDAKVVGFDQDKDVAVLR 216

Query: 192 INS-KSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIVSCVDRKSSDLGLGGIRR 251
           I++ K+ L    +G S+ L  G  V AIG P  L +T+T G++S + R+ S    G   +
Sbjct: 217 IDAPKNKLRPIPVGVSADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQ 276

Query: 252 EYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDD---AVGLSFAVPIDSVSKITEQFKK 311
           + +QTD AIN GNSGGPL++  G ++G+N         + G+ F++P+D+V  I +Q  +
Sbjct: 277 DVIQTDAAINPGNSGGPLLDSSGTLIGINTAIYSPSGASSGVGFSIPVDTVGGIVDQLVR 336

Query: 312 RGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPGSPASRAGFRS---- 371
            G+V RP LG+K     +  +EQL            GVL+    P  PA +AG +S    
Sbjct: 337 FGKVTRPILGIKFAP--DQSVEQLG---------VSGVLVLDAPPSGPAGKAGLQSTKRD 396

Query: 372 -------GDVVIELDSHPVASIKEIIEIM-----GDRVGVP-LKAVVKRSVNSTITLTVL 415
                  GD++  ++   V++  ++  I+     GD V V  L+   K  +  ++TL   
Sbjct: 397 GYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEVTVEVLRGDHKEKI--SVTLEPK 439

BLAST of MS007305 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 122.9 bits (307), Expect = 6.3e-28
Identity = 91/301 (30.23%), Postives = 157/301 (52.16%), Query Frame = 0

Query: 132 GSGTIIDKDGTILTCAHVVTGFYS---SRAVSKGKVEVTLQDG--RTFEGTVMNADFHSD 191
           GSG + D  G I+T  HV+    S   S     G+V +   DG  + FEG ++ AD   D
Sbjct: 155 GSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKD 214

Query: 192 IAIVKINS-KSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIVSCVDR---KSSD 251
           +A++K+++ ++ L   K+G S+ L+ G   +AIG P    +T+T G++S ++R     + 
Sbjct: 215 LAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG 274

Query: 252 LGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVN---IMKVDDAVGLSFAVPIDSVS 311
           + +GG     +QTD AIN GNSGGPL++  G ++G+N     +   + G+ FA+P  +V 
Sbjct: 275 VTIGG----GIQTDAAINPGNSGGPLLDSKGNLIGINTAIFTQTGTSAGVGFAIPSSTVL 334

Query: 312 KITEQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPGSPASRA 371
           KI  Q  +  +V+R  + +++    + +  QL  R+        G  +A      P SR 
Sbjct: 335 KIVPQLIQFSKVLRAGINIELAP--DPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSR- 394

Query: 372 GFRS----GDVVIELDSHPVASIKEIIEIMGD-RVGVPLKAVVKRSVNSTITLTVLPEES 416
           GF      GD+++ +D  PV +  E+++I+ +  VG  +   +KR  N  + L +  EE 
Sbjct: 395 GFAGNIVLGDIIVAVDDKPVKNKAELMKILDEYSVGDKVTLKIKRG-NEDLELKISLEEK 447

BLAST of MS007305 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 110.9 bits (276), Expect = 2.5e-24
Identity = 88/298 (29.53%), Postives = 151/298 (50.67%), Query Frame = 0

Query: 132 GSGTIIDKDGTILTCAHVVTGFYS---SRAVSKGKVEVTLQDG--RTFEGTVMNADFHSD 191
           GSG + D  G I+T  HV+    S   S     G+V +   DG  + FEG ++ AD   D
Sbjct: 155 GSGVVWDGQGYIVTNYHVIGNALSRNPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKD 214

Query: 192 IAIVKINS-KSPLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIVSCVDR---KSSD 251
           +A++K+++ ++ L   K+G S+ L+ G   +AIG P    +T+T G++S ++R     + 
Sbjct: 215 LAVLKVDAPETLLKPIKVGQSNSLKVGQQCLAIGNPFGFDHTLTVGVISGLNRDIFSQTG 274

Query: 252 LGLGGIRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDDAVGLSFAVPIDSVSKIT 311
           + +GG     +QTD AIN GNSGGPL++  G ++G+N            A+   +V KI 
Sbjct: 275 VTIGG----GIQTDAAINPGNSGGPLLDSKGNLIGINT-----------AIFTQTVLKIV 334

Query: 312 EQFKKRGRVIRPWLGLKMIDLNEMIIEQLKERDTTFPAVTEGVLIAMVTPGSPASRAGFR 371
            Q  +  +V+R  + +++    + +  QL  R+        G  +A      P SR GF 
Sbjct: 335 PQLIQFSKVLRAGINIELAP--DPVANQLNVRNGALVLQVPGKSLAEKAGLHPTSR-GFA 394

Query: 372 S----GDVVIELDSHPVASIKEIIEIMGD-RVGVPLKAVVKRSVNSTITLTVLPEESN 416
                GD+++ +D  PV +  E+++I+ +  VG  +   +KR  N  + L +  EE +
Sbjct: 395 GNIVLGDIIVAVDDKPVKNKAELMKILDEYSVGDKVTLKIKRG-NEDLELKISLEEKS 433

BLAST of MS007305 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 90.9 bits (224), Expect = 2.6e-18
Identity = 64/176 (36.36%), Postives = 96/176 (54.55%), Query Frame = 0

Query: 132 GSGTIIDKDGTILTCAHVVTGFYSSR-AVSKGKVEVTLQDGRTF--EGTVMNADFHSDIA 191
           GSG + DK G I+T  HV+    + +  + + KV +    G  F  EG ++  D  +D+A
Sbjct: 131 GSGFVWDKLGHIVTNYHVIAKLATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLA 190

Query: 192 IVKINSKS-PLPMAKLGSSSKLRPGDWVVAIGCPLSLQNTVTAGIVSCVDRKSSDLGLGG 251
           ++KI ++   L    LG+S+ LR G    AIG P   +NT+T G+VS + R+      G 
Sbjct: 191 VLKIETEGRELNPVVLGTSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPSPN-GK 250

Query: 252 IRREYLQTDCAINVGNSGGPLVNIDGEVVGVNIMKVDD-----AVGLSFAVPIDSV 299
              E +QTD  IN GNSGGPL++  G  +GVN           + G++FA+PID+V
Sbjct: 251 SISEAIQTDADINSGNSGGPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTV 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150785.11.1e-22395.10putative protease Do-like 14 [Momordica charantia][more]
XP_022965762.12.4e-19184.00putative protease Do-like 14 isoform X1 [Cucurbita maxima][more]
XP_022965763.12.4e-19184.00putative protease Do-like 14 isoform X2 [Cucurbita maxima][more]
KAG6586251.13.5e-19082.86putative protease Do-like 14, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022938300.13.0e-18982.63putative protease Do-like 14 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q3E6S87.0e-13360.00Putative protease Do-like 14 OS=Arabidopsis thaliana OX=3702 GN=DEGP14 PE=3 SV=2[more]
A9JRB38.7e-5938.12Serine protease HTRA1B OS=Danio rerio OX=7955 GN=htra1b PE=2 SV=1[more]
Q9D2361.5e-5839.43Serine protease HTRA3 OS=Mus musculus OX=10090 GN=Htra3 PE=1 SV=3[more]
F1N1522.5e-5840.64Serine protease HTRA1 OS=Bos taurus OX=9913 GN=HTRA1 PE=2 SV=1[more]
Q927432.5e-5840.64Serine protease HTRA1 OS=Homo sapiens OX=9606 GN=HTRA1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D9G45.2e-22495.10putative protease Do-like 14 OS=Momordica charantia OX=3673 GN=LOC111018849 PE=3... [more]
A0A6J1HPX71.2e-19184.00putative protease Do-like 14 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465... [more]
A0A6J1HL691.2e-19184.00putative protease Do-like 14 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465... [more]
A0A6J1FCS01.4e-18982.63putative protease Do-like 14 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1FJD31.4e-18982.63putative protease Do-like 14 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G27660.13.8e-12660.69Trypsin family protein with PDZ domain [more]
AT3G27925.17.1e-3232.24DegP protease 1 [more]
AT5G39830.16.3e-2830.23Trypsin family protein with PDZ domain [more]
AT5G39830.22.5e-2429.53Trypsin family protein with PDZ domain [more]
AT4G18370.12.6e-1836.36DEGP protease 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001940Peptidase S1CPRINTSPR00834PROTEASES2Ccoord: 141..153
score: 42.96
coord: 249..266
score: 57.59
coord: 271..288
score: 30.89
coord: 211..235
score: 53.95
coord: 360..372
score: 32.08
IPR001478PDZ domainSMARTSM00228pdz_newcoord: 314..401
e-value: 3.9E-5
score: 33.1
IPR001478PDZ domainPFAMPF13180PDZ_2coord: 314..383
e-value: 1.6E-9
score: 38.1
IPR001478PDZ domainPROSITEPS50106PDZcoord: 300..378
score: 9.9333
IPR036034PDZ superfamilyGENE3D2.30.42.10coord: 311..415
e-value: 1.4E-20
score: 75.0
IPR036034PDZ superfamilySUPERFAMILY50156PDZ domain-likecoord: 313..411
NoneNo IPR availableGENE3D2.40.10.120coord: 94..310
e-value: 5.6E-69
score: 233.8
NoneNo IPR availablePFAMPF13365Trypsin_2coord: 132..278
e-value: 6.3E-33
score: 114.8
NoneNo IPR availablePANTHERPTHR22939:SF125SERINE PROTEASE HTRA2, MITOCHONDRIALcoord: 77..418
NoneNo IPR availablePANTHERPTHR22939SERINE PROTEASE FAMILY S1C HTRA-RELATEDcoord: 77..418
NoneNo IPR availableCDDcd00987PDZ_serine_proteasecoord: 314..409
e-value: 6.81343E-15
score: 67.6648
IPR009003Peptidase S1, PA clanSUPERFAMILY50494Trypsin-like serine proteasescoord: 98..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS007305.1MS007305.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0005515 protein binding
molecular_function GO:0004252 serine-type endopeptidase activity